269 results about "Berberine" patented technology

The invention discloses a reagent and a method for detecting berberine-resistant viruses. The reagent provided by the invention comprises an antibody or an antigen binding fragment thereof, the antibody or the antigen binding fragment thereof comprises a heavy chain complementarity determining region HCDR1-3 and / or a light chain complementarity determining region LCDR1-3, the heavy chain complementarity determining region HCDR1-3 has a sequence as shown in SEQ ID NO.5-7, and the light chain complementarity determining region LCDR1-3 has a sequence as shown in SEQ ID NO.8-10. The reagent can be used for detecting the berberine-resistant herpes simplex virus type 1, so that the treatment effect can be evaluated in advance before berberine is applied for treatment.

The invention discloses a preparation method and application of a double-layer sound transmission type berberine-loaded gelatin microsphere slow-release antibacterial gel dressing, and belongs to the technical field of gel dressings. The preparation method comprises the following steps: taking vinyl silicone oil and silicon dioxide as raw materials, preparing upper-layer silica gel by adopting a double-component uniform mixing method, and then laminating the upper-layer silica gel and lower-layer berberine-loaded gelatin microsphere hydrogel by adopting a self-laminating method, thereby obtaining the double-layer sound-transmission type berberine-loaded gelatin microsphere slow-release antibacterial gel dressing. By means of the broad-spectrum antibacterial function of berberine, the efficient drug loading function and slow drug release function of gelatin microspheres, the sound transmission and swelling functions of lower hydrogel and the supporting function of upper hydrogel, ultrasonic inspection is conducted through a dressing covering layer, an ultrasonic scanning supporting plane is provided, ultrasonic waves penetrate through the dressing, good imaging is formed, and the application prospect is wide. And antibacterial and repairing effects on local wounds are realized at the same time.

The present application relates to the technical field of small molecule drugs, in particular to a isoquinoline drug and its application in improving or treating digestive tract tumors, and aims to provide a compound, a pharmaceutical composition containing the compound and its application in treating colorectal cancer and gastric cancer, and provides a 3,13-position substituted berberine derivative, the drug itself has less toxicity and does not affect the growth of normal human cells, and has a good application prospect in the development of novel antitumor drugs, especially in the development of anti-digestive tract tumor drugs.

A nutraceutical composition, consisting of Nicotinamide Mononucleotide in a range 250-1000 mg, Spermidine in a range 0.5-10 mg, Rhodiola Rosea in a range 200-600 mg, Cacao in a range 1000-10,000 mg, L-Theanine in a range 50-200 mg, Glucosamine in a range 500-3000 mg, and Hyaluronic acid in a range 50-240 mg, or consisting of Epigallocatechin-3-gallate in a range 100-500 mg, Quercetin in a range 250-1000 mg, Resveratrol in a range 150-1000 mg, Sulforaphane in a range 1-50 mg, and Calcium Alphaketoglutarate in a range 300-2000 mg, or consisting of Microdose Lithium in a range 1-5 mg, Trimethylglycine in a range 500-2000 mg, Glutathione in a range 250-1000 mg, Fisetin in a range 100-1000 mg, Urolithin A in a range 250-2000 mg, Sodium Butyrate in a range 100-3000 mg, Berberine in a range 500-1500 mg, Apigenin in a range 50-500 mg, Cinnamon in a range 1000-6000 mg, and Malate in a range 1000-6000 mg.

The invention belongs to the field of nano-medicine and traditional Chinese medicine, and particularly discloses an antioxidant nano-material based on self-assembly of traditional Chinese medicine and copper ions and a preparation method thereof.The nano-material is prepared from BPCu nano-particles formed by self-assembly of berberine and procyanidine B2 under induction of the copper ions, the BPCu nano-particles are further subjected to surface modification through polyvinylpyrrolidone, and the antioxidant nano-material is prepared. And the stably dispersed PVP-coated BPCu nano structure is obtained. The material is small in particle size, uniform in distribution, good in water solubility and storage stability, excellent in free radical scavenging capacity and catalase-like activity and suitable for adjuvant therapy of oxidative stress related diseases, and the preparation method is easy and convenient to operate, does not need an organic solvent and has good industrialization and disease treatment potential.

The invention discloses a nano-drug material based on self-assembly of berberine and caffeic acid and a preparation method of the nano-drug material. The preparation method comprises the following steps: respectively dissolving berberine and caffeic acid in an organic solvent to prepare a berberine-organic solvent solution and a caffeic acid-organic solvent solution; adjusting the pH value of the caffeic acid-organic solvent solution to 7.0-7.5; adding the caffeic acid-organic solvent solution while stirring the berberine-organic solvent solution, uniformly mixing, adding the mixed solution into deionized water with the preheating temperature of 30-70 DEG C, and stirring at a constant temperature, so that the berberine and the caffeic acid are self-assembled to obtain a mixed solution; dialyzing the mixed solution to obtain a berberine and caffeic acid self-assembled solution; and performing vacuum freeze drying to obtain a target product. The nano-drug material disclosed by the invention is small in particle size, better in solubility and dissolution rate in different pH environments than berberine hydrochloride and caffeic acid eutectic, good in stability in an aqueous solution and environment-friendly in method.

The invention discloses a photocuring microneedle with ginseng exosome loaded berberine, a preparation method of the photocuring microneedle and application of the photocuring microneedle to tissue repair. A ginseng exosome loaded berberine compound is prepared through a microfluidic technology; filling a needle cavity of a microneedle mold with a needle tip layer preparation solution containing the compound, methylacryloylated hyaluronic acid and methylacryloylated gelatin through a centrifugal method; covering a backing layer preparation solution composed of hyaluronic acid and povidone K90, and centrifuging again; and finally, carrying out light curing, drying and demolding. The prepared composite microneedle is complete and sharp in needle shape and high in forming rate; the efficient entrapment of the active medicine is realized; the biocompatibility is good, and the irritation is low; a cross-linked network structure formed by photocuring is beneficial to realizing controlled release of drugs, the targeted repair function of the ginseng exosome is combined with the hypoglycemic effect of berberine, and a new strategy and technical approach with a prospect are provided for painless, efficient and long-acting treatment of diabetes mellitus.

The invention relates to the field of organic chemistry, and particularly discloses a synthetic method of berberine hydrochloride. The synthesis method of berberine hydrochloride comprises the following steps: S1, carrying out reductive amination reaction on bromohomopiperony lamine and o-veratraldehyde to synthesize an intermediate II; s2, carrying out Suzuki coupling reaction on the intermediate II and an organic boron compound under the catalysis of a palladium metal organic matter to synthesize an intermediate III; s3, the intermediate III and organic chloride are subjected to an amino chlorination reaction, and an intermediate IV is synthesized; s4, carrying out intramolecular atom transfer radical addition and intramolecular Friedel-Crafts alkylation cascade reaction on the intermediate IV in a mixed solvent under the catalysis of a copper catalyst to synthesize an intermediate V; and S5, carrying out oxidative aromatization reaction on the intermediate V and iodine under the catalysis of alkali to synthesize berberine hydrochloride. The synthesis process is simpler, reaction conditions are easier to control, the yield is high, and the method is suitable for industrial production.

The invention discloses a preparation process of berberine hydrochloride, and belongs to the technical field of berberine production. The invention relates to a berberine hydrochloride preparation process, which comprises: S1, mixing a ferric sulfate-aluminum sulfate composite salt, sodium chloride, zinc chloride and anhydrous formic acid, pre-activating, adding N-2, 3-dimethoxybenzyl homopiperony lamine hydrochloride, dropwise adding a glyoxal mixed solution, and carrying out a reflux reaction. And S2, cooling the reaction liquid until a solid is separated out, filtering, adding a filter cake and diatomite into purified water, stirring, adjusting alkali, heating, and continuously stirring for 0.5-1 hour. Cooling and filtering, and reserving filtrate; s3, adjusting the acidity of the filtrate, cooling and growing crystals; and filtering, rinsing a filter cake, and carrying out vacuum drying on the filter cake to obtain berberine hydrochloride. The berberine hydrochloride prepared by the method is high in yield and purity, and the preparation process is safe, short in flow and friendly to human bodies and environment.

The invention discloses a self-assembled PROTAC nano material based on natural product mitochondria targeting, which is a nano particle prepared from mitochondria targeting molecules, a photosensitizer and PROTAC as raw materials through a nano precipitation method. The mitochondrial targeting molecule is berberine, the photosensitizer is hypericin, and the PROTAC is dBET57; the ratio of the amount of substance of the dBET57 to the amount of substance of the berberine is (1-10): (1-10), and the ratio of the amount of substance of the dBET57 to the amount of substance of the hypericin is (1-10): (1-10). The nano material can be used for blocking multiple energy metabolism pathways of tumor cells, including glycolysis and oxidative phosphorylation, meanwhile, mitochondria is damaged, ferroptosis is induced, and the anti-tumor capacity of pharmacodynamic molecules is improved; bRD4 in tumor cells and downstream carcinogenic protein c-Myc of the BRD4 can be efficiently degraded. The invention also discloses an application of the nano material in preparation of antitumor drugs.

The invention discloses a composite plant polypeptide composition with the effects of repairing pancreas and reducing blood sugar. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 10 to 50 parts of ginsenoside, 5 to 30 parts of radix astragali seu hedysari extract, 3 to 20 parts of radix rehmanniae polysaccharide, 2 to 15 parts of radix trichosanthis alkaloid, 5 to 25 parts of rhizoma dioscoreae polysaccharide, 5 to 30 parts of bitter gourd polypeptide, 3 to 20 parts of folium mori alkaloid, 8 to 40 parts of rhizoma polygonati polysaccharide, 5 to 25 parts of fructus hippophae flavone, 10 to 35 parts of wheat germ peptide, 2 to 15 parts of coptis berberine, 10 to 45 parts of corn stigma polysaccharide and 3 to 20 parts of saussurea involucrata polyphenol. 1-10 parts of sophora flavescens total alkaloids, 5-25 parts of a dendrobium extract, 5-30 parts of a radix puerariae extract, 8-35 parts of L-arabinose and 10-40 parts of small molecule peptides; 20 to 100 parts of collagen peptide and 15 to 80 parts of albumin peptide; 5-50 parts of microcrystalline cellulose, 3-30 parts of fructo-oligosaccharide and 0.5-5 parts of magnesium stearate; the limitation of single sugar control or simple component mixing in the prior art is broken through, a synergistic system of plant active components and functional polypeptides is constructed, and the purity and retention rate of the plant active components are improved by optimizing the extraction process.

The invention discloses a synthetic method of photosensitive composite hydrogel and application of the photosensitive composite hydrogel in treatment of periodontitis. The composition expression of the photosensitive composite hydrogel is MHA-B-coated Lipo, and the photosensitive composite hydrogel is prepared by the following steps: entrapping berberine hydrochloride by lipidosome with proton gradient, and then mixing with methacrylated hyaluronic acid, a photoinitiator and a cross-linking agent; according to the liposome with the proton gradient, a citric acid buffer solution is adopted for preparing blank liposome, and then alkali liquor is used for adjusting the pH value of an external phase to be alkalescent, so that the transmembrane gradient is formed. The liposome is used as a drug delivery carrier, berberine is entrapped in the liposome and is combined with the MHA hydrogel to form a composite sustained-release drug, and the composite sustained-release drug has the advantages of strong targeting property, good responsiveness, good biocompatibility and high biological safety; and the hydrogel has injectable and photosensitive cross-linking characteristics, is suitable for clinical minimally invasive operation, has the advantages of accurate immune regulation and control, and has a wide application prospect.

The invention relates to the technical field of food packaging, in particular to a dual-antioxidant packaging film as well as a preparation method and application thereof. The dual antioxidant packaging film disclosed by the invention is prepared from the following components in parts by mass: 3.2 to 4 parts of starch, 0.8 to 1 part of glycerol, 0.8 to 1 part of polysaccharide, 0.02 to 0.16 part of berberine / anthocyanin self-assembled nanoparticles and 93.98 to 95.2 parts of water. The packaging film prepared by the invention is uniform in structure, has good mechanical properties and moisture and oxygen barrier properties, shows an intelligent slow release characteristic in a pH value change or oxidation environment, can realize controllable release of the tea tree essential oil, and significantly improves the antioxidant and antibacterial activity of the film. The material source is natural, renewable and biodegradable, and the environment-friendly fresh-keeping packaging material is suitable for fresh-keeping packaging of foods such as fruits, vegetables and meat products, and has good environment friendliness and industrial application prospects.

The invention discloses a composition for treating intractable skin infection, sores and ulcers and inflammation as well as a preparation method and application of the composition, and belongs to the technical field of functional compositions. The composition is prepared from the following components in parts by mass: 1 to 5 parts of baicalin, 0.5 to 3 parts of antibacterial alkaloid, 2 to 6 parts of asiaticoside, 3 to 8 parts of salvianolic acid B, 1 to 4 parts of gallic acid, 5 to 10 parts of rhizoma bletillae polysaccharide, 3 to 10 parts of konjac glucomannan, 3 to 5 parts of chitosan, 8 to 20 parts of glycerol, 0.5 to 2 parts of genipin, 1 to 3 parts of calcium chloride, 1 to 3 parts of laurocapram and 40 to 80 parts of water. The antibacterial alkaloid comprises berberine or phellodendrine. Based on deep understanding of an intractable skin pathological mechanism, the components are scientifically matched, and the composition shows a remarkable synergistic effect in in-vitro antibacterial, anti-biofilm and anti-inflammatory experiments. The preparation process is simple, low in cost and suitable for industrial production.

The invention discloses a composition for preventing and treating mastitis and its preparation method and application, including the following components in parts by mass: 10-12 parts of aloe gel, 3-5 parts of berberine tannate and 2-4 parts of gentiana macrophylla extract. The aloe gel and the gentiana macrophylla extract in the present composition are both plant extract ingredients, and both have anti-inflammatory and bactericidal effects. Berberine tannate is a kind of alkaloid, has significant antibacterial effect, and the three are compounded, and the use of antibiotics can be effectively reduced, thereby reducing the residual of antibiotics in animal products, and increasing the safety of product; in addition, aloe gel, gentiana macrophylla extract and berberine tannate have synergistic synergistic effect, and the three are compounded, and mastitis can be effectively treated.

This invention relates to the field of plant component extraction technology, specifically to a method for efficiently extracting and purifying berberine. The method includes: (1) selecting dried stems of *Radermachera sinensis*, crushing and sieving them to obtain *Radermachera sinensis* powder; (2) weighing the *Radermachera sinensis* powder and mixing it with a 20-30% (w / w) ammonium sulfate solution, then adding a compound enzyme preparation for enzymatic hydrolysis, adding isopropanol to obtain a mixture, extracting the mixture using an ultrasonic-microwave synergistic extraction method, centrifuging the extracted mixture for 10-13 min at 3500-4000 rpm, removing the isopropanol layer by distillation to obtain a crude extract; (3) dissolving the crude extract in water and loading it onto a polar macroporous resin for multiple elutions to obtain berberine. Compared with the prior art, this invention uses an enzyme preparation followed by an aqueous two-phase solvent combined with ultrasonic-microwave extraction technology to promote the maximum release of berberine from *Radermachera sinensis* powder, thereby increasing the yield. Finally, a gradient elution with macroporous resin is used to achieve efficient extraction and purification of berberine.

The invention discloses a short-contact washing agent for treating scalp seborrheic dermatitis and bacterial folliculitis, and relates to the technical field of external preparations and scalp care, the short-contact washing agent comprises the following components by weight: an active component combination: 0.01-0.05 wt% of halcinonide; 0.5 to 1.0 wt% of piroctone ethanolamine; 0.1 to 0.3 wt% of total alkaloids of sophora flavescens; 0.05 to 0.1 wt% of berberine; standardized traditional Chinese medicine effective part extracts are compounded with modern active ingredients, and the problem that the quality difference between batches of traditional Chinese medicine lotion is large is solved. The combination of a plurality of active components aims to synergistically act on a plurality of targets such as anti-inflammation, antibiosis and oil control, so as to comprehensively improve the scalp condition. In addition, the specific semi-solid colloid dosage form improves the staying performance of the product on the scalp, and aims to improve the refreshing feeling after use while ensuring the cleaning effect. The lotion provides a hormone-containing and hormone-free double-version design, and provides choices for requirements of different severity degrees and different use stages.

This invention discloses a method for preparing a chitosan antibacterial film. First, berberine aqueous solution and tannic acid aqueous solution are prepared separately. Then, the berberine aqueous solution is added dropwise to the tannic acid aqueous solution to form a mixed solution. The mixed solution is stirred and heated to obtain a suspension. The suspension is freeze-dried to obtain berberine-tannic acid nanoparticles. Next, a chitosan acetic acid aqueous solution is mixed with the berberine-tannic acid nanoparticles, and a film is formed to obtain a chitosan antibacterial film loaded with berberine-tannic acid nanoparticles. The prepared chitosan antibacterial film has low water content and swelling degree, strong ultraviolet shielding ability, high transparency, and strong inhibitory activity against Staphylococcus aureus and Salmonella typhimurium, which can better meet the needs of food preservation.

The invention discloses an application of berberine in treatment of neurame disease. Specifically, the berberine achieves the effect of preventing and treating the Lyme disease or the neurolyme disease by regulating ADRB2. The ADRB2, as a key regulatory gene, plays an important role in pathopoiesis and treatment of neurame disease.

The invention discloses application of berberine or berberine hydrochloride in preparation of drugs for resisting HPV infection related diseases. Preferably, the berberine is an extract of natural plants such as coptis chinensis, golden cypress, berberis poiretii schneid or Chinese mahonia. According to the application, the new application of the berberine or the hydrochloride thereof in the aspect of resisting the HPV virus is expanded, and the berberine or the hydrochloride thereof is used for preparing the preparation for resisting the HPV infection, so that diseases related to the HPV infection can be prevented or treated, and the virus is promoted to become negative. As a natural plant extract, berberine is high in safety and not easy to generate drug resistance, and has important clinical significance when being used for preparing Chinese patent medicine products for resisting HPV infection.

The present disclosure relates to berberine alpha-ketoglutarate salts, which can be used in a pharmaceutical or nutraceutical composition for the treatment or prevention of bacterial infections, cardiovascular diseases, or other conditions. The present disclosure also relates to crystalline forms, methods of preparing berberine alpha-ketoglutarate salts and applications thereof.

The invention provides an anti-cancer medicine and food homologous composition and a preparation method thereof. The composition is prepared from the following components in parts by weight: 5 to 10 parts of lipoic acid, 30 to 50 parts of nano curcumin, 2 to 7 parts of berberine, 50 to 100 parts of beta-glucan, 15 to 35 parts of polymethylacrylic acid, 15 to 25 parts of herba hedyotidis diffusae extract, 7 to 15 parts of scutellaria barbata baicalein, 25 to 55 parts of astragalus polysaccharide, 8 to 18 parts of coix seed ester and 10 to 15 parts of cordyceps cicadae. Through a space-time targeting synergistic release mechanism of a bionic nanocellulose skeleton and in combination with pH / enzyme double-response intelligent regulation and control, three-stage precise synergistic effects of oxidation resistance, apoptosis promotion and immune activation are realized, the limitation that a traditional medicinal and edible composition is poor in targeting property, low in synergistic efficiency and uncontrollable in toxicity is broken through, and the medicinal and edible composition has a broad application prospect. High-efficiency and low-toxicity tumor multi-channel synergistic intervention is achieved in a natural component system for the first time, and the actual effect of the whole medicinal and edible composition on cancer and swelling resistance is improved.

1. Name of the product in this design: Pharmaceutical Packaging Box (Huangtengsu Capsules). 2. Purpose of this design: This design is used for packaging berberine capsules. 3. The key design features of this product are the combination of pattern and color. 4. The image or photograph that best illustrates the design's key points: a 3D model. 5. The design for which protection is sought includes color.

The present application belongs to the technical field of guava disease prevention and treatment, and particularly relates to a kind of fungicide composition for preventing and treating guava anthracnose.A kind of fungicide composition for preventing and treating guava anthracnose, its effective component is compounded by rock white menu lactone and berberine or flutriafol, wherein, the mass ratio of rock white menu lactone and berberine is 1-7:15-1;The mass ratio of rock white menu lactone and flutriafol is 1-20:20-1.Rock white menu lactone and berberine or flutriafol are compounded in the present application, and the synergistic coefficient SR for inhibiting the mycelium growth of guava anthracnose pathogenic bacteria is greater than 1.5, which shows synergistic effect.Compared with single component, the prevention and treatment effect on guava anthracnose can be improved.In addition, the fungicide composition of the present application is compounded by rock white menu lactone and berberine or flutriafol, compared with single component, the generation and development of pathogenic bacteria resistance can be delayed, and the service life of the pesticide can be prolonged.

The invention provides a pharmaceutical composition for treating prostatitis and a preparation method thereof. The pharmaceutical composition is prepared from the following raw materials in parts by weight: 20 to 60 parts of palmatine, 20 to 60 parts of paeoniflorin, 10 to 30 parts of formononetin, 20 to 60 parts of astilbin and 10 to 40 parts of stigmasterol. In-vitro cell tests and in-vivo animal experiments prove that the pharmaceutical composition has the effect of relieving the chronic prostatitis reaction.

This invention belongs to the field of pharmaceutical technology, specifically relating to biomimetic nanomedicines for treating subcutaneous drug-resistant bacterial infections, their preparation methods, and applications. In this invention, dopamine hydrochloride is added during the synthesis of a metal-organic framework to prepare a dandelion-shaped carbon nanozyme precursor. This precursor is then carbonized to obtain a carbon nanozyme with targeting, photoresponsiveness, and peroxidase activity. Finally, berberine hydrochloride is loaded onto the carbon nanozyme to obtain the biomimetic nanomedicine. The biomimetic nanomedicine provided by this invention firstly possesses good biocompatibility; secondly, it has a unique dandelion-like morphology, which is beneficial for its targeting effect; and finally, it exhibits strong photoresponsiveness in the near-infrared region, enabling synergistic and efficient bactericidal action through the mechanisms of antibacterial drugs, photothermal therapy, and chemokinetics. It has significant application value in the preparation of drugs for treating subcutaneous drug-resistant bacterial infections.

The invention belongs to the technical field of microbial biopesticides, and particularly relates to a high-efficiency, low-toxicity and environment-friendly microbial pesticide, which is characterized in that effective components of the high-efficiency, low-toxicity and environment-friendly microbial pesticide are formed by compounding microbial active components and compounding factors; wherein the microbial active component is P.megatherium; the microbial active component is P.megatherium; the compound factor is physcion or berberine. The bacterium content of the microbial active component, namely, the preristeria megatherium, is 5.0 * 10 < 10 > cfu / g, and the preristeria megatherium has a relatively good inhibition effect on the growth of fungal pathogenic bacteria mycelia and has the potential of being developed into a microbial bactericide; when the compound is compounded with the existing biopesticide physcion or berberine, the compounded composition has a synergistic effect on the growth of fungal pathogenic bacteria hyphae of crops, and compared with the single use of the microbial active component, the compound composition can improve the prevention and treatment effect on diseases, is beneficial to reducing the application amount of the pesticide, and has a good application prospect. And the method is of great significance to development of compound microbial biopesticides.

This invention discloses a pharmaceutical composition containing the natural products osthol and berberine, exhibiting synergistic antibacterial and antifungal effects. The effectiveness of osthol in synergistically enhancing the antibacterial activity of berberine was demonstrated through checkerboard minimum inhibitory concentration (MIC) tests, antifungal activity experiments, and in vitro bacterial growth curves. This invention provides a novel application of the combined use of osthol and berberine in antibacterial and antifungal treatments. This composition belongs to the category of traditional Chinese medicine compound preparations, offering a new treatment strategy for the clinical treatment of bacterial and fungal infectious diseases.

The invention relates to the technical field of hemostatic materials, in particular to antibacterial and anti-inflammatory silicon carbide ore-based multifunctional hemostatic powder and a preparation method thereof. The preparation method of the silicon carbon ore-based multifunctional styptic powder comprises the following steps: S1, dispersing epsilon-polylysine and silicon carbon ore in water, then freeze-drying and grinding into powder; s2, the epsilon-polylysine modified silicon carbon ore is dispersed in water, and a Pl-EPL solution is prepared; s3, adding the Pl-EPL solution and the berberine solution into the polyacrylic acid solution, and uniformly mixing; and then, under a stirring condition, dropwise adding a polyethyleneimine solution into the mixed solution, freeze-drying, and grinding into powder. The hemostatic material disclosed by the invention can participate in the blood coagulation process more efficiently, exert antibacterial and antioxidant effects, reduce inflammatory response, realize multiple-effect synergy of hemostasis, antibacterial, anti-inflammatory and antioxidant, and solve the limitation of a single-function hemostatic material.