315 results about "Salvianolic acid" patented technology

The invention discloses a method for preparing salvianolic acid A by catalytically converting salvianolic acid B. The method is characterized in that the converted raw material is a salvia miltiorrhiza aqueous extract (salvianolic acid B=>50%) primarily purified through combined chromatography; the concentration of the raw material salvianolic acid B is 0.5-2%; urea is taken as the catalyst; the mole ratio of urea to the salvianolic acid B is 0.3-0.7; the conversion reaction temperature is 100-125 DEG C; and the reaction time is 3-6 hours. The method has the following beneficial effects: urea is taken as the catalyst, thus greatly shortening the time for which the salvianolic acid B is in easily destroyed state and remarkably increasing the yield of the salvianolic acid A; the primarily purified salvia miltiorrhiza extract is taken as the converted raw material, thus not only removing the metal ions which are not beneficial to conversion but also removing most colloid-like impurities and frontal impurities which are not beneficial to following separation of the salvianolic acid; and the directional conversion rate of the salvianolic acid B to the salvianolic acid A prepared by the method is not less than 10% and even reaches 60%.

The invention provides a red sage root mask. The red sage root mask is prepared by adding a mask matrix and water into total salvianolic acid utilized as an active ingredient, wherein, the matrix comprises the following components in parts by weight: 4.9-14.7 parts of the total salvianolic acid, 22.4-67.2 parts of a film-forming material, 7.8-23.4 parts of a thickening agent, 4.9-14.7 parts of a skin penetration enhancer, 0.25-0.75 parts of an antioxidant and 1-3 parts of a preservative. The invention further provides a preparation method of the red sage root mask. The red sage root mask is a peel-off mask, wherein, the total salvianolic acid has an effect on promoting body surface microcirculation, thus smoothening the body surface blood circulation, accelerating the blood flow velocity, fully moisturizing and nourishing skin and hair and ensuring the stability of water-soluble components of red sage root. Therefore, the red sage root mask can be widely used in health and skin care.

The invention is concerned with a kind of extract of total ketone of salviae miltiorrhizae and total phenolic acid and its produce method form radix Salviae Miltiorrhizae. The extract of total ketone of salviae miltiorrhizae has cryptotanshinone, tanshinone I, tanshinone IIA, methyl Tanshinon, dihydrotanshinon I and ramification. The extract of total phenolic acid has salvianolic acid A, salvianolic acid B, protocatechuic aldehyde and ramification. The extract can be got form one or arbitrary compound of extraction with solvent method, macroporous resin method, column chromatography and liquid-liquid counter-current chromatography. The summation of the content to each total ketone of salviae miltiorrhizae is 20 to 100 percnte (w / w) of the extract of total ketone of salviae miltiorrhizae, the contene of cryptotanshinone, tanshinone I and tanshinone IIA is 5 to 100 percent (w / w) of whole content of total ketone of salviae miltiorrhizae. The summation of the content to each total phenolic acid is 5 to 100 percent (w / w) of the extract of the radix salviae miltiorrhizae total phenolic acid. The content of salvianolic acid B is the 5 to 100 percent (w / w) of the whole salvianolic acid.

The invention discloses an extraction method of salvianolic acid B and hydroxy safflor yellow A as well as a preparation method of danhong for injection, and relates to the extraction method of the salvianolic acid and the hydroxy safflor yellow as well as the preparation method of the danhong preparation. The invention solves the problems that the extraction of the salvianolic acid B and the hydroxy safflor yellow A is not thorough, and the transportation and the storage of the prepared danhong injection are inconvenient. The salvianolic acid B of the invention is extracted according to the following steps: backflow, acidification and adsorption; thus the salvianolic acid B is obtained. The hydroxy safflor yellow A of the invention is extracted according to the following steps: boil out, acidification and adsorption; thus the hydroxy safflor yellow A is obtained. The danhong for injection is extracted according to the following steps: compounding ratio and standing, filtrating and lyophylization; thus the danhong for injection of the invention is obtained. The extraction rate of the salvianolic acid B and the hydroxy safflor yellow A of the invention is high, and the transportation and the storage of the prepared danhong injection are convenient.

The invention discloses a method for manufacturing salvianolic acid A of danshen root, which is characterized in that total salvianolic acid in the danshen root is transformed into salvianolic acid A by adopting a certain method. The method increases greatly the extraction yield of salvianolic acid A. The salvianolic acid A is prepared into tablets, capsule, granula, soft capsule, micro pill, dripping pill and oral liquid, which have good pharmacological action indicated by pharmacological experiments.

The invention discloses an artificial cultivation method for increasing content of an active ingredient in roots, stems and blades of red-rooted sage. In the method disclosed by the invention, a step of spraying a mixed aqueous solution of methyl salicylate and methyl jasmonate is only added on the basis of the original conventional production and planting process, and the sprayed aqueous solution is prepared by adding 0.01-0.25g of chemically pure or industrially pure methyl salicylate and 0.015-0.40g of methyl jasmonate into 100L of water and then stirring. The method is simple to implement, the investment is less, the harvest is great, the total content of salvianolic acid ingredient in a red-rooted sage plant can be greatly increased, specifically, the total content of the salvianolicacid ingredient is increased to about 10% from about 6% and is nearly twice of the original total content of the salvianolic acid ingredient, especially the content of the salvianolic acid ingredientin the stems and blades of the red-rooted sage plant can be greatly increased, specifically, the total content of the salvianolic acid ingredients is increased to about 5% from about 1.6% and is nearly more than four times of the original total content of the salvianolic acid ingredient, thus the stems and blades of the red-rooted sage also have values in development and utilization.

The invention relates to a method for furthering separating and purifying salvianolic acid from Danshen extract fluid, which comprises that prepares, breaks and extracts Danshen via water solution, acidifies extract to adjust pH and adds salt to process post-treatment, processes dynamic continuous adsorption and elution on the treated extract at chromatography column stuffed with resin adsorbent, elutes via water, collects and concentrates eluent, elutes via gradient ethanol solution, segmented collects and concentrates eluent, and dries the concentrates solution to obtain product. The invention can simply, effectively and quickly separate and purify various salvianolic acids, wherein test on different salvianolic acid products shows that the highest yields of tanshinol, alkannic acid, rosmarinic acid, salvianolic acid A and salvianolic acid B are 35. 55%, 70.65%, 99.27%, 82.78%, and 89.34%, and relative highest purities are 95.32%, 65.05%, 29.40%, 33.93%, and 82.35%, which are near or higher than the result of purification on the goal of single component.

The invention discloses a medicinal composition for preventing and treating cardiovascular diseases, belonging to the technical field of Chinese medicines. The medicinal composition is prepared from salvianolic acids, total tanshinone and a notoginsenoside mixture in a certain weight ratio, wherein the notoginsenoside mixture is prepared by mixing ginsenoside Rb1, ginsenoside Rg1 and notoginsenoside R1; and the notoginsenoside mixture can be replaced by an equivalent amount of arasaponin. The medicinal composition has remarkable treatment effects on a coronary heart disease, angina and myocardial infarction.

The invention discloses a control method for the preparation of salvianolic acid; in the method, the proper quantity of solvent is added into the salvia drinking piece or the smashed salvia to be used as the menstruum; after the extraction and the solid-liquid separation, the extracted liquid reacts at the high temperature and a high pressure; then the medical liquid is added into the chromatography column; after eluted by water, elute with the eluant; collect the high-purity salvianolic acid A solution; compress the eluant until the eluant has no organic solvent under the reduced pressure or the normal pressure; the alkali is used to adjust the pH value of the acquired solution; the solution is extracted with the chloroform or ether organic solvent; after the organic solvent are extracted, the acetic acid ester and similar organics solvents are added into the water for extraction; the medical liquid is compressed and dried with the acetic acid ester and similar organics solvents and then the salvianolic acid A product of 50 to 84 percent is gotten. Through controlling the content of the salvianolic acid A in the final extracted materials, the performance of the final extracted materials are more stable; and the invention is low in the requirements for the chemical medicine, more universal, and more suitable for the industrialization.

The invention relates to a method for increasing the contents of tanshinone and salvianolic acid in a salvia miltiorrhiza hairy root by using a transgene AtMYC2, belonging to the technical field of gene engineering. The method comprises the steps of constructing a high-efficiency expression vector of a plant by using an arabidopsis transcription factor AtMYC2, and carrying out genetic transformation on salvia miltiorrhiza leaves to obtain a gene AtMYC2 overexpressed transgenetic salvia miltiorrhiza hairy root; analyzing the expression of AtMYC2 in the transgenetic salvia miltiorrhiza hairy root and related genes in biosynthetic pathways of tanshinone and salvianolic acid through qRT-PCR; measuring the contents of tanshinone and salvianolic acid in the transgenetic salvia miltiorrhiza hairy root by using a high-performance liquid chromatography (HPLC); and measuring the antioxidant activity of tanshinone and salvianolic acid in the transgenetic salvia miltiorrhiza hairy root by using a DPPH free radical scavenging method. The invention provides the method for simultaneously increasing the contents of tanshinone and salvianolic acid in salvia miltiorrhiza hairy root and also provides a novel high-quality raw material for producing tanshinone and salvianolic acid with important clinic demands so as to have the positive promoting significance and application value for relieving the problem that the drug resources of tanshinone and salvianolic acid are short.

Compositions are provided containing molecules having at least one moiety from beta-(3,4-dihydroxyphenyl)lactic acid and / or caffeic acid, which are found in extracts from the plant genus Salvia, said moieties being of formula(I), the active agents having a molecular weight of at least 190 daltons. A class of preferred agents are those which are conjugated to form dimers, trimers, tetramers and larger polymers containing said moieties, with the most preferred being salvianolic acid dimers, trimers, tetramers and larger polymers. The compounds and compositions may be administered in pharmaceutically acceptable carriers and excipients systematically or locally to treat viral infections.

The invention relates to establishment of a salvianolic acid extract fingerprint spectrum and a content measurement method of related components. The content measurement method includes the following steps: 1) establishing the salvianolic acid extract digitized quantitative fingerprint spectrum through ultrahigh performance liquid multi-wavelength visible ultraviolet detection method; 2) recognizing peaks in the fingerprint spectrum in the step 1) with mass spectrum to determine common fingerprint peaks of ultraviolet detection; and 3) performing measurement to contents of three indicative components, which are high in content, significant in activity and definite in structure, recognized in the step 2) through high performance liquid chromatography variable-wavelength detection method.

The invention relates to extracting tanshinpropanone from the effective part of the root of red-rooted salvia and preparation thereof and application of medicinal compound from said extracting stuff in preparation of drugs for treatment and prevention for blood shortage disease of heart and brain. Process for tanshinpropanone is as follows, extracting tanshinone type compound by supercritical extracting technique of carbon dioxide, boiling with water and extracting soluble salvianolic acid type and purifying thereof through ethyl acetate, thereafter mixing these two solid powder obtained according to extracting rate and content converting.

The present invention is medicine composition for treating cerebral ischemia and its medicinal application. The medicine composition has ginsenoside and salvianolic acid as main components. It can reduce the cerebral infarction area obviously, reduce behavior disorder caused by cerebral tissue necrosis obviously, and reach the aim of treating cerebral blood supply insufficiency, cerebral thrombosis, cerebral embolism, cerebral vascular spasm, senile dementia, etc.

The present invention relates to one kind of phenolic acid compound and its preparation process and use in treating AIDS. Red sage material is treated through solvent extraction, macroporous adsorbing resin purification, conventional drying and other steps to obtain total phenolic acid. The total phenolic acid is dissolved in water via heating, chromatographic column separation and repeated purification to obtain new compound salvianolic acid N. Enzyme and cell activity experiments show that the new compound has HIV resisting activity and is hopeful in being developed into one new kind of AIDS treating medicine.

The invention discloses a preparation method of a high-content salvianolic acid B. The method comprises the following two steps: separating total salvianolic acids and preparing the high-content salvianolic acid B. According to the method, water phase is selectively removed via a method of organic solvent extraction without affecting on water-soluble components, and the rest of water phase is acidized and then extracted, so that the water-soluble components with high polarity such as inorganic salt, tannin, sugar and protein and can be selectively removed; finally, the high-content total salvianolic acid is obtained. Based on the obtained total salvianolic acid, salvianolic acid B product having content being more than 95% is finally obtained with the final yield about 3% by carrying out the steps of regulating pH of the total salvianolic acid solution within 6.0 to 8.0, separating the total salvianolic acid solution via macroporous resin and the like. The method is simple to develop, effective and low in cost, and has an industrial value.

The invention relates to new use of B Salvianolic acid. The B Salvianolic acid has a strong inhibition effect to tyrosinase, thereby being used for whitening skin, removing freckle, resisting wrinkle, and treating hyperpigmentation diseases, and being applicable to food additives. The inhibition of the B Salvianolic acid to the activity of the tyrosinase is detected, the B Salvianolic acid is very high in the activity of the tyrosinase, and is better in stability, and the B Salvianolic acid can effectively inhibit the generation of the melanin, and can be taken as the natural skin-whitening agent for cosmetics. Meanwhile, the B Salvianolic acid can more preferably promote the skin cell proliferation function, and has the functions of beautifying and resisting wrinkle, thereby being applicable to the cosmetics with the functions of beautifying and resisting wrinkle.

The invention provides a manufacturing method of red phenolic acid B magnesium salts. Crushes the radix salviae miltiorrhizae, immerses and extracts with alcohol containing water or acetone, condenses, eliminates the sediment. Then uses macropore to absorb resin chromatography, washes them with water, then wash with alcohol. The coarse product is obtained after condensed, then carries on chromatography with polyamide, and washes with water or alcohol. There gets the 90% purity product through condensation, cooling, drying.

The invention provides a preparation method of a Salvia miltiorrhiza soft capsule, comprising the following steps of: completely mixing Salvia miltiorrhiza extract with glycerine and polyethylene glycol 400 so that liposoluble constituents such as tanshinone II A are dissolved in a liquid substrate so as to achieve certain solubilization and improve the biological availability of liposoluble constituents of Salvia miltiorrhiza. In the preparation process of the Salvia miltiorrhiza soft capsule, a method for preparing Salvia miltiorrhiza water-soluble component extract is provided, a macroporous resin separation technology is adopted, and ethanol solution is concentrated, atomized and dried after elution; in the obtained extract, the total salvianolic acid compounds account for more than 60% of total solid, wherein the content of salvianolic acid compound danshinolic acid B is between 45% and 60%, and the content of danshensu is controlled between 10% and 20%. The content of danshensu is controlled while the yield of salvianolic acids is improved so that the proportion of the main active ingredient danshinolic acid B is obviously improved. The preparation method is safe and efficient, low-toxic and environmentally-friendly, can satisfy the requirement of industrial production, and has wide and practical application value.

The present invention provides salvianolic acid A derivative and its application as antioxidant based on the physical and chemical properties and bioactivity of natural salvianolic acid. The salvianolic acid A derivative has the pharmacological effects of resisting oxidation, resisting inflammation, protecting endothelial cell, lowering blood fat, raising HDL, etc. It may be applied in preventing and treating atheroscleorsis, cardiac and cerebral vascular diseases, rheumatoid arthritis, diabetes, tumor and other diseases.

The invention belongs to the medicine technical field, in particular to a gastric retention preparation containing total salvianolic acid. The invention adopts the effective position of the Chinese medicine of total salvianolic acid as the main component, and the stomach retention preparation is made with acceptable auxiliary materials in pharmacy. The preparation of the invention is gastric floating preparation or stomach mucoadhesive preparation; the invention can significantly prolong the retention time of the total salvianolic acid on the stomach and the upper part of small intestine, enhance stability of medicine, and prolong effective absorption time and improve the utilizing degree of salvianolic acid biology through the double effect of enhancing the medicine stability and prolonging the effective absorption time. The invention can not only improve gastrointestinal absorption, but also have better efficacy in treating gastric ulcer and some other diseases; the invention also has simple production technique and is easy for industrialization; the invention is easy for the patients to take with good compliance.

The composition of Chinese medicine effective parts for treating cardiac and cerebral vascular diseases consists of general salvianolic acid and general astragalus saponin in certain ratio. The compound medicine has raised pharmacological effect and clinical curative effect and reduced impurity content. It may be prepared into various preparation forms. It is used in treating coronary heart disease, angina pectoris, myocardial ischemia, heart failure, arrhythmia, cerebral infarction and cerebral ischemia; and is safe, effective stable, controllable and convenient.

The invention relates to a method for determining the content of water in Salvianolic acid for injection through near-infrared diffuse reflection spectrometry. The method comprises a step of establishing a near-infrared quantitative correction model, and a step of determining the content of water in a sample.

The invention provides a medicinal composition containing general ginsenoside and total salvianolic acid and a preparation method thereof. The preparation method comprises the following steps of: performing heating reflux on ginseng in 60 percent (volume / volume) of aqueous solution of ethanol, after recovering the ethanol, allowing extract to pass through macroporous adsorbent resin, eluting by using water, 20 percent (volume / volume) aqueous solution of ethanol and 60 percent (volume / volume) aqueous solution of ethanol respectively, collecting a part which is eluted by 60 percent (volume / volume) aqueous solution of ethanol, and removing solvent to obtain general ginsenoside extract; leaching root of red-rooted salvia in water by heating, adding the ethanol into the extract until the volume of the ethanol is 80 percent of that of the extract, standing, filtering, after recovering the ethanol, allowing filter liquor to pass through the macroporous adsorbent resin, eluting by using the water and 40 percent (volume / volume) of aqueous solution of ethanol, collecting a part which is eluted by 40 percent (volume / volume) of aqueous solution of ethanol, and removing the solvent to obtain total salvianolic acid extract; and mixing the general ginsenoside extract and the total salvianolic acid extract according to a weight ratio of (0.1-10):(0.1-10).

The invention provides a method for detecting tannin substance in salvianolic acid. The method for detecting tannin substance in salvianolic acid comprises the following steps of: firstly, preparing a reference solution; secondly, dissolving salvianolic acid to obtain an unhydrolyzed sample solution before hydrolysis; thirdly, dissolving salvianolic acid and then hydrolyzing, so as to obtain a hydrolyzed sample solution; fourthly, determining gallic acid, catechinic acid and ellagic acid in the unhydrolyzed sample solution, the hydrolyzed sampled solution and the reference solution; and fifthly, comparing chromatograms and judging whether the tannin substance exists in salvianolic acid.

The invention discloses a method for extracting salvianolic acid A, which is characterized in that a new process method is adopted to obtain salvianolic acid A extract, the yield of the salvianolic acid A in the salvianolic acid A extract prepared with the method is more than five thousandth, thus saving a great quantity of resources. The invention further discloses a method for preparing the salvianolic acid A, which comprises the steps of adopting a medium pressure liquid phase preparation method, wherein eluent has two phases to carry out isocratic or gradient elution to obtain high-purity salvianolic acid A with related substances, such as salvianolic acid F and salvianolic acid C, with content lower than 0.5 percent. The quality of the salvianolic acid A obtained by using the method is more uniform and more stable.

The present invention relates to the technical field of medicine, specifically to an application of salvianolic acid C in preparation of drugs for prevention and treatment of hyperuricemia. The results of the pharmacological test show that: the salvianolic acid C can be adopted as a xanthine oxidase (XO) inhibitor to prevent and treat hyperuricemia and complications of the hyperuricemia, wherein the complications caused by the hyperuricemia comprise: gout, gouty arthritis, gouty nephropathy, lithangiuria, cardiovascular diseases and other diseases.

The invention provides a water-soluble salvia miltiorrhiza extractive, wherein the weight percent of total phenolic acid is 80-100 percent. The water-soluble salvia miltiorrhiza extractive is characterized by comprising the following components in percent by weight: 40-90 percent of salvianolic acid B, 3.0-15 percent of rosmarinic acid, 2-10 percent of alkannic acid and 0.2-2.2 percent of salvianolic acid E. The invention also provides a preparation containing the extractive and the extractive.

The invention relates to application of polymer salvianolic acid in preparation of drugs used for inhibiting occurrence or development of aortic aneurysm or aortic dissection and a pharmaceutical composition comprising the polymer salvianolic acid used as a pharmaceutical active substance, related desired adjuvant and the like. The pharmaceutical composition can be used for inhibiting occurrence of aortic aneurysm or aortic dissection. The polymer salvianolic acid is dimer salvianolic acid, trimer salvianolic acid or tetramer salvianolic acid, wherein the dimer salvianolic acid is rosmarinic acid, the trimer salvianolic acid is salvianolic acid C, alkannic acid or salvianolic acid A, and the tetramer salvianolic acid is salvianolic acid B.

The invention provides a red sage root acne-removing repairing cream. For extraction of a red sage root extract, ethanol with concentrations of 90% and 15% is employed; extraction temperature is 65 DEG C; extraction time is 40 min; the dose (a mass-to-volume ratio) of an extraction solvent for extraction is 1: 10 each time; and extraction is carried out three times. The extracted optimal effective parts of red sage root are a 90% ethanol red sage root extract and a 15% ethanol red sage root extract, and the mean values of the anti-propionibacterium acne activity of the two extracts are 16.7 + / - 0.4 mm and 16.7 + / - 0.5 mm, respectively. The mean value of the activity of a mixture composed of the two extracts in a mass ratio of 5: 2 is increased to 18.9 + / - 0.3 mm. The mixture can be used as an acne-removing effective part of red sage root, and the material basis of the mixture are tanshinone and salvianolic acid. The red sage root acne-removing repairing cream is prepared from the mixture and other materials in proportion. The red sage root acne-removing repairing cream prepared in the invention is a natural excellent health care product for the skin, has substantial efficacy, does not result in allergy or other adverse reaction and is good news for patients with acne.