36 results about "Salvianolic acid" patented technology

The invention provides a content detection method for a salvia miltiorrhiza medicinal material, a salvia miltiorrhiza clear paste and a musk heart-dredging dropping pill. According to the method, a high performance liquid chromatography method is adopted, octadecylsilane chemically bonded silica is used as a chromatographic column filler, acetonitrile is used as a mobile phase A, 0.02%-0.1% trifluoroacetic acid is used as a mobile phase B, gradient elution is carried out, and rosmarinic acid and salvianolic acid B in the salvia miltiorrhiza medicinal material are synchronously and quantitatively detected. And synchronously and quantitatively detecting tanshinol, protocatechuic aldehyde, caffeic acid, salvianolic acid E, rosmarinic acid, alkannic acid and salvianolic acid B in the salvia miltiorrhiza clear paste, and synchronously and quantitatively detecting tanshinol, protocatechuic aldehyde, caffeic acid, salvianolic acid E, rosmarinic acid, alkannic acid, salvianolic acid B, arenobufagin and bufalin in the musk Tongxin dropping pill. The method is used for monitoring the content of specific components, can also judge the transfer rate in the medicine production process, and facilitates traceability in the production link. In addition, the method is relatively low in equipment requirement and suitable for industrial popularization.

The invention relates to an efficient guiding editing system for salvia miltiorrhiza and application of the efficient guiding editing system, and belongs to the technical field of gene editing. The invention provides a guided editing system suitable for salvia miltiorrhiza. The guided editing system comprises a U6complex promoter, a fusion protein and gRNA (guide Ribonucleic Acid), the fusion protein is a PEmax-MLH fusion protein; the nucleotide sequence for compiling the PEmax-MLH fusion protein is as shown in SEQ ID NO. 1; and the gRNA is pegRNA and sgRNA (small guide RNA). The constructed guided editing system realizes diversified and accurate editing in stable transformation of plants, the single-base replacement efficiency reaches 61.0%, the complex insertion replacement efficiency reaches 2.2%, the six-base replacement efficiency reaches 11.6%, and meanwhile, experiments prove that after the guided editing system is adopted to edit salvia miltiorrhiza, the accuracy of the edited salvia miltiorrhiza is greatly improved. The content of tanshinone and salvianolic acid of the edited plant is obviously improved.

Provided is the use of a traditional Chinese medicine composition in preparation of a drug for preventing major adverse cardiac and cerebrovascular events after percutaneous coronary intervention and ameliorating myocardial injury and vascular endothelial dysfunction after percutaneous coronary intervention. The traditional Chinese medicine composition is composed of total salvianolic acid and total hawthorn triterpene acid.

The present invention belongs to the field of pharmaceutical synthesis, and specifically relates to salviphorol derivatives and their application in drugs for treating cancer cachexia. Salviphorol compounds include compounds represented by formula (X) and / or formula (Y), or pharmaceutically acceptable salts or optical isomers of the compounds represented by formula (X) and / or formula (Y). The present invention also provides a pharmaceutical composition for treating cachexia diseases, and its application in the preparation of drugs for treating cancer cachexia, including muscle atrophy caused by tumor tissues, fat reduction caused by tumor tissues, reduced appetite caused by tumor tissues, inflammatory responses caused by tumor tissues, and cancer cachexia caused by digestive tract-related cancers, liver cancer, lung cancer, and colon cancer. The present invention also provides its application in the preparation of drugs or inhibitors for treating or inhibiting muscle atrophy, drugs or inhibitors for inhibiting or alleviating adipocyte lipolysis, and drugs or inhibitors for inhibiting or alleviating weight loss or reduction.

The invention provides application of strontium ions in improving and / or promoting accumulation of tanshinone components and / or salvianolic acid components, and belongs to the technical field of hairy root induction. The invention provides application of strontium ions in improving and / or promoting accumulation of tanshinone components and / or salvianolic acid components. Salvia miltiorrhiza hairy roots are induced through different strontium ion concentrations, the influence of strontium ions on salvia miltiorrhiza quality components is researched, and a theoretical basis is expected to be provided for salvia miltiorrhiza quality improvement and strontium fertilizer development. The result of the embodiment shows that 10-100 [mu] mol / L of strontium ions are added in the salvia miltiorrhiza hairy root induction process, accumulation of tanshinone components and salvianolic acid components in the salvia miltiorrhiza hairy roots can be improved and promoted, and then the quality components of salvia miltiorrhiza can be improved.

The present disclosure provides a medicament for treating brain diseases. Specifically, provided is a pharmaceutical composition. The active ingredient of the pharmaceutical composition comprises: (a) a first active ingredient, wherein the first active ingredient is a pharmaceutically acceptable salt of salvianolic acid; and (b) a second active ingredient, wherein the second active ingredient is ginsenoside or a pharmaceutically acceptable salt thereof. The pharmaceutically acceptable salt of salvianolic acid is selected from the following group: magnesium salvianolate B, calcium salvianolate B, magnesium salvianolate A, sodium rosmarinate, and a combination thereof. The ginsenoside is selected from the following group: ginsenoside Rg1, ginsenoside Rh2, ginsenoside Rb1, and a combination thereof. The mass ratio of the first active ingredient to the second active ingredient is 1:(0.1-10), wherein the mass ratio is based on the pharmaceutically acceptable salt of salvianolic acid and ginsenoside. Also provided is use of the pharmaceutical composition in preventing or treating brain diseases.

The invention belongs to the technical field of medicines, and particularly relates to a tanshinone and salvianolic acid compound molecular compatibility composition for regulating and controlling the balance relation of DNMTs / TET2 and application of the tanshinone and salvianolic acid compound molecular compatibility composition. An in-vitro cell model proves that the composition with specific compatibility can synergistically regulate the expression of DNMTs and TET2 and recover the balance relation of DNMTs and TET2, so that the abnormal DNA methylation state is reversed, the normal expression of related genes is recovered, and the composition can be used for preparing the medicine for preventing or treating cardiovascular diseases related to DNA methylation abnormity. The molecular compatibility composition for balance regulation and control of key targets is created for the first time, has the advantages of clear component compatibility, clear action targets and synergistic interaction, and provides a new choice for developing novel epigenetic regulation and control drugs.

The present disclosure provides a use of salvianolic acid E (SAE) in the preparation of drugs targeting senescent cells, inhibiting tumors or prolonging lifespan. The inventors are committed to research in screening drugs targeting tumor microenvironment, enhancing the anti-tumor effect of chemotherapeutic drugs, eliminating senescent cells or restraining cellular senescence. Here, it is revealed that salvianolic acid E exerts its effects by targeting tumor microenvironment and eliminating senescent cells. When combined with chemotherapeutic drugs, it shows extremely significant effects in promoting tumor suppression by removing senescent stromal cells. For a senescence-associated secretory phenotype (SASP), the SAE can also effectively target senescent cells so as to inhibit the SASP. Besides, the SAE can also significantly prolong the life of animals, significantly extend the survival period of the old and improve the life quality of animals.

The application discloses a freeze-dried mask based on Yuyong Powder and a preparation method thereof, and the preparation method comprises the following steps: after raw materials of the Yuyong Powder are crushed at low temperature, the raw materials are extracted at low temperature by using a composite solvent, and then the extracted solution is refined by flocculation clarification, centrifugation and microfiltration to obtain a refined extraction solution; the refined extraction solution is mixed with a humectant and a freeze-drying protective agent, and then is injected into a mask support, and gradient temperature control freeze-drying is carried out; finally, the freeze-dried mask is sealed and packaged under sterile conditions by using nitrogen. Through the whole low-temperature process and the synergistic effect of the composite freeze-drying protective agent, the heat-sensitive whitening components such as paeoniflorin, salvianolic acid and glabridin in the Yuyong Powder are effectively retained; impurities are removed through three-stage refining and purification, so that the irritability of the product is reduced; through freeze-drying and high-barrier packaging, the product can be stored at room temperature for a long time. The obtained freeze-dried mask can be quickly reconstituted when meeting water, has a refreshing skin feeling, and has remarkable effects of removing yellow and brown spots and whitening, and has the advantages of high activity, high stability, high convenience and high safety.

The invention relates to an efficient salvia miltiorrhiza single base editing system and application thereof, and belongs to the technical field of base editing. The invention provides a salvia miltiorrhiza single base editing system. The salvia miltiorrhiza single base editing system comprises a 35SEN composite promoter, sgRNA, a SmRPS5A promoter and a fusion protein, the fusion protein is a SmABE8e fusion protein or an SmAKBE fusion protein. According to the invention, efficient salvia miltiorrhiza single base editing systems SmABE8e-03 and SmAKBE-03 are successfully constructed, and bases of salvia miltiorrhiza can be edited; in addition, the salvia miltiorrhiza single base editing system can edit termination codons of 5 'UTR and SmKSL2 proteins of the SmMYB1 gene, and the content of tanshinone and salvianolic acid in salvia miltiorrhiza can be increased under the condition that growth traits of the salvia miltiorrhiza are not affected by destroying uATG, ORF and protein structures.

The present application relates to a kind of efficient salvia miltiorrhiza single base editing system and its application, belong to base editing technical field.The present application provides a kind of salvia miltiorrhiza single base editing system, the salvia miltiorrhiza single base editing system includes 35SEN complex promoter, sgRNA, SmRPS5A promoter and fusion protein;The fusion protein is SmABE8e fusion protein or SmAKBE fusion protein.According to the present application, efficient salvia miltiorrhiza single base editing system SmABE8e-03 and SmAKBE-03 are successfully constructed, can edit the base of salvia miltiorrhiza, and salvia miltiorrhiza single base editing system can edit the 5'UTR of SmMYB1 gene and the termination codon of SmKSL2 protein, by destroying uATG, ORF and protein structure, can improve the content of tanshinone and salvianolic acid in salvia miltiorrhiza under the condition of not affecting its growth trait.

The invention relates to a preparation method and a quality control method of a salvia miltiorrhiza standard extract containing salvianolic acid, tanshinone and polysaccharide components. The preparation method of the salvia miltiorrhiza standard extract specifically comprises the following steps: (1) taking salvia miltiorrhiza decoction pieces, adding 10 times of 80% ethanol, performing reflux extraction for 3 times, 1 hour each time, and combining filtrates; (2) adding 9 times of pure water into decoction dregs, decocting for 3 times, 1.5 hours each time, and combining filtrates; and (3) concentrating the combined filtrate at 60 DEG C under reduced pressure to a certain volume, transferring to a freeze-drying machine, freeze-drying, and crushing to obtain the traditional Chinese medicine composition. The invention simultaneously establishes a fingerprint spectrum of the salvia miltiorrhiza standard extract and a method for determining the content of tanshinone, salvianolic acid and polysaccharide components in the salvia miltiorrhiza standard extract, and establishes a method for determining the molecular weight of the polysaccharide components in the salvia miltiorrhiza standard extract and analyzing the monosaccharide composition and content in the salvia miltiorrhiza standard extract, so as to control the quality of the salvia miltiorrhiza standard extract.

The invention relates to a total synthesis method of salvianolic acid F. The method comprises the following steps: brominating 2, 3-dimethoxybenzaldehyde to obtain 2-bromine-5, 6-dimethoxybenzaldehyde, carrying out Wittig reaction to obtain (E)-1-bromine-2-(3, 4-dimethoxystyryl)-3, 4-dimethoxybenzene, and carrying out reaction on (E)-1-bromine-2-(3, 4-dimethoxystyryl)-3, 4-dimethoxybenzene to obtain the salvianolic acid F. The preparation method comprises the following steps: taking salvianolic acid as a raw material, reacting with acrylate under the action of alkali and a palladium catalyst to obtain tetramethyl salvianolic acid F, and obtaining salvianolic acid F from the tetramethyl salvianolic acid F and a demethylation reagent. The preparation method is short in route and reasonable in process, the adopted reagent is safe, low in cost and free of environmental pollution, meanwhile, the preparation method has the advantages of simplicity in operation, easiness in separation and the like, and the obtained product is high in yield.

The invention discloses a salvia miltiorrhiza hole tray factory substrate seedling raising method. The method comprises the following steps: a) preparing a substrate; b) hole tray treatment; c) seed pretreatment; d) sowing and accelerating germination; e) culturing by stages; f) seedling hardening and outplanting. According to the seedling raising method for the salvia miltiorrhiza hole tray factory substrate, through a layered substrate structure and precise temperature and light control, the emergence rate and the seedling rate are greatly increased, a light-temperature cooperation strategy is adopted, low light and low temperature are achieved in the first stage, the transpiration effect is reduced, long growth caused by water roots (water absorption > transpiration) of salvia miltiorrhiza seedlings is avoided, strong light and carbon enrichment are achieved in the second stage, and the light saturation point is increased under the high CO2 concentration; accumulation of secondary metabolites (salvianolic acid) is accelerated, the seedling raising efficiency is improved, the seedling raising period is shortened, early-stage soil-borne diseases are blocked through seed coating agent enveloping, fungal spores are killed through KMnO4 plug tray disinfection, pythium propagation is inhibited through matrix low-EC value management, and the infection resistance of seedling cell walls is enhanced through regular spraying of KH2PO4, so that a disease and insect pest prevention and control closed loop is achieved.

The invention relates to a SmTSR gene and application of overexpression of the SmTSR gene in improving the content of salvianolic acid and tanshinone in salvia miltiorrhiza and improving the drought tolerance of the salvia miltiorrhiza. The nucleotide sequence of the SmTSR gene is shown as SEQ ID No.3, and the amino acid sequence of protein coded by the SmTSR gene is shown as SEQ ID No.4; the invention further provides a method for cultivating the salvia miltiorrhiza material overexpressing the SmTSR gene. The SmTSR gene has the advantages that through overexpression of the SmTSR gene, the drought tolerance of the radix salviae miltiorrhizae is remarkably enhanced, the content of salvianolic acid and tanshinone in the radix salviae miltiorrhizae is increased, and the SmTSR gene is of great significance in improving the quality of the radix salviae miltiorrhizae; the construction method is low in technical difficulty and high in operability, the production cost of the salvianolic acid tanshinone can be better reduced, the drought tolerance of the radix salviae miltiorrhizae is improved, and the method has a good application prospect.

This invention discloses the SmWRKY13 gene and its application in increasing the salvianolic acid content in Salvia miltiorrhiza, belonging to the field of genetic engineering technology. By constructing a recombinant expression vector containing the SmWRKY13 gene and transforming it into hairy roots of Salvia miltiorrhiza, transgenic hairy root lines overexpressing SmWRKY13 were obtained. The salvianolic acid content of these transgenic hairy root lines was detected. The results showed that overexpression of SmWRKY13 significantly increased the content of salvianolic acid components such as rosmarinic acid and salvianolic acid B in the hairy roots of Salvia miltiorrhiza, with the total salvianolic acid content reaching up to 5.72 times that of the control group. This invention provides an effective strategy for increasing the salvianolic acid content in Salvia miltiorrhiza using genetic engineering methods, which is of great significance for meeting market demand and promoting the large-scale preparation of salvianolic acid.

The application discloses histone deacetylase SmHDA6 and application of an encoding gene thereof in regulating accumulation of salvianolic acid. The application provides a histone deacetylase SmHDA6 for regulating biosynthesis of salvianolic acid in Danshen, wherein an amino acid sequence is shown as SEQ ID NO. 4, and a nucleotide sequence is shown as SEQ ID NO. 1. In the application, the SmHDA6 has a Histone deacetylase domain conservative functional domain for exerting histone deacetylation, is clustered in a Type I (RPD3-like superfamily) subfamily in the HDA family, expression amounts of the SmHDA6 gene in different parts of Danshen are obviously positively correlated with salvianolic acid content, and expression of the SmHDA6 gene is significantly up-regulated after being induced by ABA, MeJA and SA. Knocking out the SmHDA6 gene by a gene editing technology can significantly improve salvianolic acid content in Danshen, because knocking out the SmHDA6 gene activates expression of genes in a salvianolic acid metabolic pathway, and then increases biosynthesis of the salvianolic acid. Therefore, the SmHDA6 can regulate accumulation of the salvianolic acid in Danshen, can be applied to engineering bacteria and genetic engineering breeding of Danshen with high salvianolic acid content, and has great application value.

The invention provides application of titanium ions in promoting accumulation of tanshinone IIA and / or salvianolic acid components of salvia miltiorrhiza, and belongs to the technical field of hairy root induction. The invention provides application of titanium ions in improving and / or promoting accumulation of salvia miltiorrhiza tanshinone IIA and / or salvianolic acid components. According to the method, hairy roots of salvia miltiorrhiza are induced by using different titanium ion concentrations, and the influence of titanium ions on quality components of salvia miltiorrhiza is researched. The result of the embodiment shows that 2-10 [mu] mol / L of titanium ions are added in the salvia miltiorrhiza hairy root induction process, accumulation of tanshinone IIA and salvianolic acid components in salvia miltiorrhiza hairy roots can be improved and promoted, and then the quality components of salvia miltiorrhiza can be improved. Through the influence of titanium ions on the quality components of the salvia miltiorrhiza, a theoretical basis is provided for improving the quality of the salvia miltiorrhiza and developing a titanium fertilizer.

The invention relates to the field of medicine emplastrum, and discloses a nanometer emplastrum and a preparation method thereof.The nanometer emplastrum comprises 55%-60% of nanometer particles and 40%-45% of traditional Chinese medicine active ingredients; the nano-particles comprise 25%-30% of animal protein extract, 15%-20% of mineral protein extract and 50%-55% of a nano-carrier base material, the animal protein extract comprises fish scale collagen; the mineral protein extract comprises diatomite protein; the traditional Chinese medicine active components comprise 12%-15% of an agaric polysaccharide extract, 10%-15% of a panax notoginseng saponins extract, 5%-10% of a safflower flavone extract, 8%-12% of a salvianolic acid extract and 5%-10% of malic acid. According to the invention, a porous nano-carrier constructed by animal protein and mineral protein breaks through the bottleneck of transdermal efficiency of a traditional plaster, and based on the characteristic of high specific surface area of fish scale collagen and diatomite protein composite nanoparticles and in combination with the pH regulation effect of malic acid, the transdermal permeability of macromolecular active components such as auricularia auricula polysaccharide, notoginsenoside and the like is remarkably improved.

The invention discloses a compound foliar fertilizer as well as a preparation method and application thereof, and belongs to the technical field of agricultural fertilizers. The invention provides a compound foliar fertilizer. The compound foliar fertilizer takes trehalose and SmCEP3 micromolecule polypeptide as core active ingredients; the amino acid sequence of the SmCEP3 small-molecule polypeptide is as shown in SEQ ID NO: 1. The method is applied to the aspect of red-rooted salvia root biomass increase, solves the problem of low active ingredient content in the existing red-rooted salvia, and can directionally and synergistically improve the contents of various medicinal active ingredients such as tanshinone and salvianolic acid in the red-rooted salvia.

The invention discloses a pharmaceutical composition for cardiovascular and cerebrovascular diseases, and relates to the technical field of traditional Chinese medicines, the pharmaceutical composition comprises the following raw materials by weight: 150-250 parts of panax notoginseng saponins, 8-12 parts of ginkgo total flavonoids, 3-5 parts of dipyridamole, 80-120 parts of a salvianolic acid extract, 5-15 parts of astragaloside, 20-40 parts of a composition of saikoside a and albiflorin, and 30-50 parts of a composition of ginsenoside Rg1 and ophiopogonin D; according to the pharmaceutical composition, the system treatment rule of eliminating, clearing, lowering, warming, harmonizing and tonifying in the traditional Chinese medicine is completely and quantitatively applied to the design of the modern traditional Chinese medicine compound composition for the first time, so that the abstract theory is converted into specific quantifiable components and proportions, and the problem that the traditional compound is not clear and unclear is solved; all the components in the composition are safe components which are recorded in pharmacopoeia or are clinically used for a long time, and potential risks caused by large-dose use of a single component can be reduced through reasonable compatibility and mutual restriction.

The invention relates to the technical field of biological medicine, in particular to application of salvianolic acid in preparation of an anti-tuberculosis drug. The invention provides application of salvianolic acid A or pharmaceutically acceptable salt thereof in preparation of the anti-tuberculosis drug, the molecular formula of the salvianolic acid A is C26H22O10, and the structural formula is shown in formula I. When the salvianolic acid A is applied to preparation of the anti-tuberculosis drug, a brand new material basis is provided for research and development of the anti-tuberculosis drug, damage of the drug to normal tissues of a human body is reduced, and the anti-tuberculosis drug can be applied to preparation of the anti-tuberculosis drug. Toxic and side effects are reduced. From the aspect of action mechanism, the salvianolic acid A has various biological activities such as inflammation resistance, oxidation resistance and immunoregulation, and can inhibit the growth and reproduction of mycobacterium tuberculosis and enhance the immune defense capability of the body to tubercle bacillus through the multi-target synergistic effect, thereby achieving the purpose of treating tuberculosis.

The invention provides a medicine for treating cerebral diseases. Specifically, the invention provides a pharmaceutical composition, and the active components of the pharmaceutical composition comprise (a) and (b): (a) a first active component which is a pharmaceutically acceptable salt of salvianolic acid; and (b) a second active ingredient, wherein the second active ingredient is ginsenoside or a pharmaceutically acceptable salt thereof; wherein the pharmaceutically acceptable salt of salvianolic acid is selected from the following group: salvianolic acid B magnesium salt, salvianolic acid B calcium salt, salvianolic acid A magnesium salt, rosmarinic acid sodium salt or a combination thereof; the ginsenoside is selected from the following group: ginsenoside Rg1, ginsenoside Rh2, ginsenoside Rb1 or a combination thereof; moreover, the weight ratio of the first active component to the second active component is 1: (0.1-10), and the weight ratio is based on the pharmaceutically acceptable salt of salvianolic acid and ginsenoside. The invention also relates to the use of the pharmaceutical composition in the prevention or treatment of cerebral diseases.

The application provides a salvia miltiorrhiza transcription factor SmNAC39 gene and application thereof in increasing content of salvianolic acid in salvia miltiorrhiza, and belongs to the technical field of genetic engineering.The application independently studies the influence of SmNAC39 on the content of salvianolic acid in salvia miltiorrhiza, finds that SmNAC39 can directly and positively regulate the synthesis of salvianolic acid, and provides a new idea for increasing the content of salvianolic acid in salvia miltiorrhiza and a new path for solving the problem of tight supply of salvianolic acid.

The application discloses a coding gene sequence of a Danshen bHLH transcription factor SmbHLH92 for regulating synthesis of salvianolic acid; the SmbHLH92 gene provided by the application has a nucleotide sequence shown in SEQ ID No. 1, and the gene coding protein has an amino acid sequence shown in SEQ ID No. 2. Subcellular localization experiments show that SmbHLH92 is located in the nucleus; the application constructs a SmbHLH92-RNAi carrier, genetically transforms Danshen, and obtains a transgenic hairy root; compared with a control strain (a strain obtained by transforming an RNAi empty carrier), the content of four kinds of phenolic acid components in the SmbHLH92-RNAi strain is significantly increased. Real-time fluorescent quantitative PCR results show that the expression amount of key enzyme genes of the salvianolic acid pathway in the SmbHLH92-RNAi strain is significantly increased. The SmbHLH92 provided by the application has a function of negatively regulating biosynthesis of salvianolic acid compounds, and the compounds show outstanding curative effects in treating cardiovascular and cerebrovascular diseases. The application provides a new research idea for improving the content of salvianolic acid compounds by using genetic engineering, and provides a target gene for carrying out Danshen excellent variety breeding.

The invention discloses lipid vesicles co-loading salvia miltiorrhiza-radix puerariae effective components, a preparation method and application, and the preparation method comprises the following steps: placing egg yolk lecithin, sodium deoxycholate, radix puerariae isoflavone, tanshinone and salvianolic acid in a round-bottom flask according to a weight ratio of 50: (22-27): 10: (0.3-0.9): 7, then adding methanol for fully dissolving, carrying out rotary evaporation under the conditions of 40 DEG C and 50 rpm / min, filtering, washing, and drying to obtain the lipid vesicles. Removing methanol to obtain a film; performing vacuum drying overnight at room temperature to obtain a dry and transparent film framework; adding ultrapure water into the membrane skeleton, and carrying out hydration treatment to obtain a liposome solution; and filtering the liposome solution with a 0.22 [mu] m cellulose acetate membrane to obtain the liposome. Through multi-component synergistic encapsulation, strong hydrophobic tanshinone is encapsulated in a phospholipid bilayer, weak hydrophobic pueraria isoflavone is distributed on a phospholipid layer or interface, and hydrophilic salvianolic acid is contained in a water phase or interface, so that the stability of a lipid vesicle system is enhanced, and the problem of low bioavailability of a preparation is solved.

The application provides application of strontium ions in improving and / or promoting accumulation of tanshinone components and / or salvianolic acid components, and belongs to the technical field of hairy root induction. The application provides application of strontium ions in improving and / or promoting accumulation of tanshinone components and / or salvianolic acid components. The application induces tanshinone hairy roots through different strontium ion concentrations, studies the influence of strontium ions on tanshinone components, so as to provide a theoretical basis for improving tanshinone quality and developing strontium fertilizer. The application shows through the results of examples that, in the process of inducing tanshinone hairy roots, adding 10-100 mu mol / L of strontium ions is beneficial to improving and promoting accumulation of tanshinone components and salvianolic acid components in tanshinone hairy roots, and further beneficial to improving tanshinone quality components.

The invention discloses application of salvianolic acid A in preparation of a medicine for improving the sperm function, and belongs to the technical field of biological medicine. SAA is polyphenol acid formed by caffeic acid and tanshinol, namely salvianolic acid through an ester bond and an enol bond, is a soluble component generated by many salvia species, is mainly extracted from salvia miltiorrhiza, and has the advantages that the salvianolic acid A is prepared from salvianolic acid A, salvianolic acid B and salvianolic acid C; sAA is one of the most abundant salvianolic acid in a salvia miltiorrhiza extract, a large number of studies show that SAA has a remarkable antioxidant effect, SAA can improve the function of in-vitro mature sperms, compared with a control group, after SAA is added, sperm intracellular calcium signals and hyperactivity are remarkably improved, and the occurrence rate of sperm acrosome reaction is also remarkably increased; the application of SAA can effectively improve the sperm function in the in-vitro fertilization process and improve the sperm motility of weak sperm patients, so that infertility is intervened and treated through simple non-invasive means such as artificial fertilization, and the fertility outcome of the patients is improved.

The invention discloses a pharmaceutical composition containing miltirone A, miltirone and salvianolic acid H, wherein the molar concentration ratio of the miltirone A to the miltirone to the salvianolic acid H is (0.1 to 200): (0.1 to 200): (0.1 to 200). According to the pharmaceutical composition disclosed by the invention, liver inflammation and liver fibrosis are inhibited by improving liver functions, reducing damage of liver cells, relieving infiltration of liver inflammatory cells and collagen deposition.

The invention provides an SmBTB1 gene and application thereof in increasing the content of tanshinone and salvianolic acid in salvia miltiorrhiza. According to the invention, BTB / POZ protein, namely SmBTB1, which interacts with plant protein kinase SmAPK1 protein is screened out through a yeast library. The method comprises the following steps: constructing an SmBTB1 gene silencing vector by using an RNA (Ribonucleic Acid) interferences technology, obtaining an SmBTB1 gene silencing hairy root strain through genetic transformation of a salvia miltiorrhiza explant, identifying a positive strain by using PCR (Polymerase Chain Reaction), and determining that the tanshinone content and the salvianolic acid content in the transgenic salvia miltiorrhiza hairy root are both improved. Experiments show that the tanshinone content and the salvianolic acid content in the silent strain are remarkably increased, the expression of the biosynthesis key enzyme gene is synchronously up-regulated, and the key regulation and control effect of the SmBTB1 gene is proved. The invention provides a method for simultaneously increasing the content of tanshinone and salvianolic acid in the hairy roots of salvia miltiorrhiza, a new thought can be provided for production of tanshinone and salvianolic acid, and the method has high research value and application prospect.