12 results about "Salvianolic acid" patented technology

Provided is the use of a traditional Chinese medicine composition in preparation of a drug for preventing major adverse cardiac and cerebrovascular events after percutaneous coronary intervention and ameliorating myocardial injury and vascular endothelial dysfunction after percutaneous coronary intervention. The traditional Chinese medicine composition is composed of total salvianolic acid and total hawthorn triterpene acid.

The application discloses a freeze-dried mask based on Yuyong Powder and a preparation method thereof, and the preparation method comprises the following steps: after raw materials of the Yuyong Powder are crushed at low temperature, the raw materials are extracted at low temperature by using a composite solvent, and then the extracted solution is refined by flocculation clarification, centrifugation and microfiltration to obtain a refined extraction solution; the refined extraction solution is mixed with a humectant and a freeze-drying protective agent, and then is injected into a mask support, and gradient temperature control freeze-drying is carried out; finally, the freeze-dried mask is sealed and packaged under sterile conditions by using nitrogen. Through the whole low-temperature process and the synergistic effect of the composite freeze-drying protective agent, the heat-sensitive whitening components such as paeoniflorin, salvianolic acid and glabridin in the Yuyong Powder are effectively retained; impurities are removed through three-stage refining and purification, so that the irritability of the product is reduced; through freeze-drying and high-barrier packaging, the product can be stored at room temperature for a long time. The obtained freeze-dried mask can be quickly reconstituted when meeting water, has a refreshing skin feeling, and has remarkable effects of removing yellow and brown spots and whitening, and has the advantages of high activity, high stability, high convenience and high safety.

The present application relates to a kind of efficient salvia miltiorrhiza single base editing system and its application, belong to base editing technical field.The present application provides a kind of salvia miltiorrhiza single base editing system, the salvia miltiorrhiza single base editing system includes 35SEN complex promoter, sgRNA, SmRPS5A promoter and fusion protein;The fusion protein is SmABE8e fusion protein or SmAKBE fusion protein.According to the present application, efficient salvia miltiorrhiza single base editing system SmABE8e-03 and SmAKBE-03 are successfully constructed, can edit the base of salvia miltiorrhiza, and salvia miltiorrhiza single base editing system can edit the 5'UTR of SmMYB1 gene and the termination codon of SmKSL2 protein, by destroying uATG, ORF and protein structure, can improve the content of tanshinone and salvianolic acid in salvia miltiorrhiza under the condition of not affecting its growth trait.

This invention discloses the SmWRKY13 gene and its application in increasing the salvianolic acid content in Salvia miltiorrhiza, belonging to the field of genetic engineering technology. By constructing a recombinant expression vector containing the SmWRKY13 gene and transforming it into hairy roots of Salvia miltiorrhiza, transgenic hairy root lines overexpressing SmWRKY13 were obtained. The salvianolic acid content of these transgenic hairy root lines was detected. The results showed that overexpression of SmWRKY13 significantly increased the content of salvianolic acid components such as rosmarinic acid and salvianolic acid B in the hairy roots of Salvia miltiorrhiza, with the total salvianolic acid content reaching up to 5.72 times that of the control group. This invention provides an effective strategy for increasing the salvianolic acid content in Salvia miltiorrhiza using genetic engineering methods, which is of great significance for meeting market demand and promoting the large-scale preparation of salvianolic acid.

The application discloses histone deacetylase SmHDA6 and application of an encoding gene thereof in regulating accumulation of salvianolic acid. The application provides a histone deacetylase SmHDA6 for regulating biosynthesis of salvianolic acid in Danshen, wherein an amino acid sequence is shown as SEQ ID NO. 4, and a nucleotide sequence is shown as SEQ ID NO. 1. In the application, the SmHDA6 has a Histone deacetylase domain conservative functional domain for exerting histone deacetylation, is clustered in a Type I (RPD3-like superfamily) subfamily in the HDA family, expression amounts of the SmHDA6 gene in different parts of Danshen are obviously positively correlated with salvianolic acid content, and expression of the SmHDA6 gene is significantly up-regulated after being induced by ABA, MeJA and SA. Knocking out the SmHDA6 gene by a gene editing technology can significantly improve salvianolic acid content in Danshen, because knocking out the SmHDA6 gene activates expression of genes in a salvianolic acid metabolic pathway, and then increases biosynthesis of the salvianolic acid. Therefore, the SmHDA6 can regulate accumulation of the salvianolic acid in Danshen, can be applied to engineering bacteria and genetic engineering breeding of Danshen with high salvianolic acid content, and has great application value.

The invention discloses a compound foliar fertilizer as well as a preparation method and application thereof, and belongs to the technical field of agricultural fertilizers. The invention provides a compound foliar fertilizer. The compound foliar fertilizer takes trehalose and SmCEP3 micromolecule polypeptide as core active ingredients; the amino acid sequence of the SmCEP3 small-molecule polypeptide is as shown in SEQ ID NO: 1. The method is applied to the aspect of red-rooted salvia root biomass increase, solves the problem of low active ingredient content in the existing red-rooted salvia, and can directionally and synergistically improve the contents of various medicinal active ingredients such as tanshinone and salvianolic acid in the red-rooted salvia.

The invention discloses a pharmaceutical composition for cardiovascular and cerebrovascular diseases, and relates to the technical field of traditional Chinese medicines, the pharmaceutical composition comprises the following raw materials by weight: 150-250 parts of panax notoginseng saponins, 8-12 parts of ginkgo total flavonoids, 3-5 parts of dipyridamole, 80-120 parts of a salvianolic acid extract, 5-15 parts of astragaloside, 20-40 parts of a composition of saikoside a and albiflorin, and 30-50 parts of a composition of ginsenoside Rg1 and ophiopogonin D; according to the pharmaceutical composition, the system treatment rule of eliminating, clearing, lowering, warming, harmonizing and tonifying in the traditional Chinese medicine is completely and quantitatively applied to the design of the modern traditional Chinese medicine compound composition for the first time, so that the abstract theory is converted into specific quantifiable components and proportions, and the problem that the traditional compound is not clear and unclear is solved; all the components in the composition are safe components which are recorded in pharmacopoeia or are clinically used for a long time, and potential risks caused by large-dose use of a single component can be reduced through reasonable compatibility and mutual restriction.

The application provides a salvia miltiorrhiza transcription factor SmNAC39 gene and application thereof in increasing content of salvianolic acid in salvia miltiorrhiza, and belongs to the technical field of genetic engineering.The application independently studies the influence of SmNAC39 on the content of salvianolic acid in salvia miltiorrhiza, finds that SmNAC39 can directly and positively regulate the synthesis of salvianolic acid, and provides a new idea for increasing the content of salvianolic acid in salvia miltiorrhiza and a new path for solving the problem of tight supply of salvianolic acid.

The application provides application of strontium ions in improving and / or promoting accumulation of tanshinone components and / or salvianolic acid components, and belongs to the technical field of hairy root induction. The application provides application of strontium ions in improving and / or promoting accumulation of tanshinone components and / or salvianolic acid components. The application induces tanshinone hairy roots through different strontium ion concentrations, studies the influence of strontium ions on tanshinone components, so as to provide a theoretical basis for improving tanshinone quality and developing strontium fertilizer. The application shows through the results of examples that, in the process of inducing tanshinone hairy roots, adding 10-100 mu mol / L of strontium ions is beneficial to improving and promoting accumulation of tanshinone components and salvianolic acid components in tanshinone hairy roots, and further beneficial to improving tanshinone quality components.

The invention provides an SmBTB1 gene and application thereof in increasing the content of tanshinone and salvianolic acid in salvia miltiorrhiza. According to the invention, BTB / POZ protein, namely SmBTB1, which interacts with plant protein kinase SmAPK1 protein is screened out through a yeast library. The method comprises the following steps: constructing an SmBTB1 gene silencing vector by using an RNA (Ribonucleic Acid) interferences technology, obtaining an SmBTB1 gene silencing hairy root strain through genetic transformation of a salvia miltiorrhiza explant, identifying a positive strain by using PCR (Polymerase Chain Reaction), and determining that the tanshinone content and the salvianolic acid content in the transgenic salvia miltiorrhiza hairy root are both improved. Experiments show that the tanshinone content and the salvianolic acid content in the silent strain are remarkably increased, the expression of the biosynthesis key enzyme gene is synchronously up-regulated, and the key regulation and control effect of the SmBTB1 gene is proved. The invention provides a method for simultaneously increasing the content of tanshinone and salvianolic acid in the hairy roots of salvia miltiorrhiza, a new thought can be provided for production of tanshinone and salvianolic acid, and the method has high research value and application prospect.

The present application relates to a kind of SmSNR gene and its application in improving the content of salvianolic acid in Salvia miltiorrhiza, the nucleotide sequence of the above-mentioned SmSNR gene is as shown in SEQ ID No.1.The SmSNR gene is cloned in Salvia miltiorrhiza for the first time in the present application, and it is found that it plays a negative regulation role in salvianolic acid biosynthesis, and the content of salvianolic acid in Salvia miltiorrhiza can be significantly improved by knocking out SmSNR gene in Salvia miltiorrhiza.The SmSNR transcription factor responding to gibberellin induction is screened by transcriptome sequencing analysis in the present application, and SmSNR gene knockout vector is constructed using CRISPR / Cas9 technology, and gene knockout hairy root is obtained by transformation, and the expression of key enzyme gene in the downstream synthesis pathway of salvianolic acid can be activated by knocking out SmSNR gene in Salvia miltiorrhiza, so as to effectively improve the content of salvianolic acid in Salvia miltiorrhiza, especially the content of salvianolic acid B and rosmarinic acid, which has high application value and research value.

The application provides application of titanium ions in promoting accumulation of tanshinone IIA and / or salvianolic acid components of Salvia miltiorrhiza, and belongs to the technical field of hairy root induction. The application provides application of titanium ions in improving and / or promoting accumulation of tanshinone IIA and / or salvianolic acid components of Salvia miltiorrhiza. The application studies the influence of titanium ions on quality components of Salvia miltiorrhiza by using different titanium ion concentrations to induce Salvia miltiorrhiza hairy roots. The application shows through the results of examples that adding 2-10 μmol / L titanium ions in the process of inducing Salvia miltiorrhiza hairy roots is beneficial to improving and promoting accumulation of tanshinone IIA and salvianolic acid components in Salvia miltiorrhiza hairy roots, and thus is beneficial to improving quality components of Salvia miltiorrhiza. The application studies the influence of titanium ions on quality components of Salvia miltiorrhiza, so as to provide a theoretical basis for improving quality of Salvia miltiorrhiza and developing titanium fertilizer.