573 results about "Chloroform" patented technology

The invention discloses a preparation method of a curing agent with biological self-healing capability, which comprises the following steps: preparing a bacillus pasteurii bacterial solution, preparing the bacillus pasteurii bacterial solution into an endophytic spore solution, immersing polyvinyl alcohol fibers into a sodium alginate / endophytic spore solution, taking out the polyvinyl alcohol fibers, immediately immersing the polyvinyl alcohol fibers into a calcium acetate solution, drying the polyvinyl alcohol fibers to obtain core fibers with a hydrogel coating, and curing the core fibers with the hydrogel coating to obtain the curing agent with biological self-healing capability. The preparation method comprises the following steps: taking magnesium phosphate cement as a raw material, immediately immersing the magnesium phosphate cement into a chloroform solution of a polystyrene and polylactic acid polymer blend, drying to obtain a coated microbial liquid, premixing urea and glucose, doping the premixed urea and glucose into the magnesium phosphate cement, uniformly stirring, adding the coated microbial liquid, and uniformly stirring to obtain the product. According to the invention, phosphate cement is adopted as the curing agent, has the advantages of high strength, short setting time, strong durability, low alkalinity, good biocompatibility and the like, and compared with the curing agent in the prior art, the curing agent not only has a good curing effect, but also has a crack self-healing function, and is especially suitable for reinforcing foundation soil.

The invention discloses a lytic bacteriophage for preventing and controlling vibrio in an aquaculture environment. The bacteriophage vBValMR42H belongs to a muscle tail bacteriophage and is insensitive to chloroform, the capsid of the bacteriophage vBValMR42H is free of lipid substances, and the preservation number is GDMCC No: 67434-B1. The bacteriophage has the characteristics of high adsorption speed, short incubation period and high cracking amount, keeps high activity in a wide range of temperature (4-55 DEG C) and pH (2-11), and has strong environmental adaptability. Genome analysis shows that the bacteriophage does not contain virulence factors, antibiotic resistance genes and lyogen related genes, and is high in biological safety. Physical development analysis shows that the method can be divided into a new genus. The bacteriophage R42H has the advantages of high splitting efficiency, good environmental stability, strong specificity, gene safety and the like, and can be used as an ideal biological prevention and control agent for preventing and controlling vibrio alginolyticus diseases in aquaculture.

The invention relates to a fiber composite material, and discloses a preparation method of a para-aramid and natural rubber high interface performance composite material, which comprises the following four steps: preparing a chloroform solution of natural rubber, preparing a nano para-aramid-natural rubber composite mixing material, preparing impregnated para-aramid, and mixing and vulcanizing. According to the preparation method, the para-aramid is effectively connected with the matrix through the'bridge 'effect of the para-aramid nanophase by a method for constructing the para-aramid nanophase in the matrix material, so that the interfacial acting force of the para-aramid is enhanced, and the high performance of the para-aramid in the composite material is fully exerted; the problems of poor interfacial compatibility with a base material, low binding force and the like caused by smooth surface and chemical inertness of the para-aramid at present are effectively solved, and the para-aramid nanophase can also remarkably improve the strength and toughness of the base material.

The invention discloses a gastric signet-ring cell carcinoma biomarker and a detection kit thereof, belongs to the technical field of biological medicines, and particularly relates to the detection kit of the gastric signet-ring cell carcinoma biomarker, which comprises a total RNA (Ribonucleic Acid) extraction reagent, an RNA purification reagent, a cDNA (Complementary Deoxyribonucleic Acid) first strand synthesis premixing reagent, a rapid fluorescent quantitative premixing reagent (SYBR Green), nuclease-free double distilled water and a specific primer. The RNA purification reagent at least comprises isopropanol, chloroform and absolute ethyl alcohol; the specific primer comprises an upstream specific primer and a downstream specific primer. The detection kit for the gastric signet-ring cell carcinoma biomarker is high in RNA purification and recovery efficiency and excellent in specificity.

The disclosure relates to a method of producing a multi-metal catalyst film and of producing a reactor system for catalytic removal of a wide variety of contaminants (for example, nitrate, nitrite, perchlorate, chlorate, chromate, selenate, chlorophenols, 2,4-D, dicamba, atrazine, trichloroacetic acid, bromochloroiodomethane, NDMA, TCE, TCA, chloroform, freons, RDX, HMX, TNT, PFOA, and PFOS) from water and wastewater. The disclosure also relates to a method of using the multi-metal catalyst for the removal of such contaminants and a system comprising the multi-metal catalyst film for removing such contaminants.

The invention discloses an egg yolk lecithin separation and extraction device and a technology thereof, and relates to the technical field of separation and extraction, the egg yolk lecithin separation and extraction device comprises: a stirring barrel, the stirring barrel is arranged on the ground; the feeding hole is fixedly formed in the top of the stirring barrel; the solvent tank is fixedly mounted at the top of the stirring barrel, and a chloroform solution is arranged in the solvent tank; the separating funnel is fixedly mounted at the bottom of the stirring barrel, and the separating funnel is used for separating an egg phosphorus solvent from a water phase; the motor is fixedly mounted at the top of the stirring barrel; the stirring rod is fixedly mounted at the output end of the motor, and a reciprocating spiral groove is formed in the surface of the stirring rod; the stirring plate is fixedly mounted at the bottom of the stirring rod; the elastic telescopic rod enables egg yolk and chloroform on the sliding plate to be mixed more sufficiently through up-down movement of the sliding plate.

The invention discloses an extraction method and application of high-molecular-weight turbellarian worm genome DNA, and belongs to the field of turbellarian worm genome extraction.The turbellarian worm genome DNA extraction is achieved by improving a lysate formula and removing ethylenediaminetetraacetic acid and increasing Mg < 2 + > on the basis of a conventional reagent in a classic Tris-HCl-phenol-chloroform method to inhibit activity of deoxyribonuclease II in a turbellarian worm body to inhibit degradation of genome DNA, and the high-molecular-weight turbellarian worm genome DNA is obtained. Meanwhile, ribonuclease A is added, RNA in cells can be degraded in the tissue cell digestion process, so that the step of two times of purification is omitted, the loss of DNA yield is reduced, experimental steps are simplified, especially the step of DNA purification is reduced, the problem of degradation in the extraction process of the turbellarian turbellarian worm genome DNA is solved, and the genome DNA yield (gt; 10 micrograms per turbellarian worm), and the turbellarian worm genome ultra-long sequencing or PacBio high-fidelity sequencing requirements are met.

The invention relates to a method for determining the content of total ginkgolic acid in a ginkgo biloba extract, which comprises the following steps: dissolving an extract sample in 50% ethanol, and extracting with chloroform. Adding a proper amount of ethanol into the water layer, and extracting with chloroform; and combining all extraction liquids, concentrating to be dry, dissolving residues by using methanol, and detecting by using a liquid chromatograph. By investigating a linear range test, a precision test, a stability test, a repeatability test and a standard recovery test of the method, the method can remarkably improve the purity and the detection sensitivity of a target object, reduce the matrix effect and solve the problem of impurity co-extraction of a traditional single solvent, so that the content detection result of ginkgolic acid is more accurate, and the method is suitable for industrial production. The method plays an important role in guaranteeing the safety of the ginkgo biloba extract product.

The invention discloses a degradable nanocellulose sustained-release drug-loaded film and a preparation method thereof, and belongs to the technical field of drug functional materials. The preparation method comprises the following steps: mixing and stirring microcrystalline cellulose and 64wt% sulfuric acid solution, centrifuging, dialyzing to be neutral, and freeze-drying to obtain CNCs; cNCs, NaIO4 and water are mixed and then subjected to a light-shielding reaction, ethanol precipitation is added, dialysis purification is conducted, freeze drying is conducted, and formylation modified CNCs are obtained; mixing and stirring the aldehyde modified CNCs, sericin, polyvinyl alcohol, water and a target drug to obtain a core layer solution; dissolving a polylactic acid-glycolic acid copolymer into a mixed solution of chloroform and DMF (Dimethyl Formamide) to obtain a shell solution; the core layer solution and the shell layer solution are subjected to coaxial electrostatic spinning forming to obtain the drug-loaded fiber, then the drug-loaded fiber is subjected to biomimetic mineralization surface modification to obtain the degradable nanocellulose sustained-release drug-loaded film, the drug loading rate is increased, the mechanical property of the drug-loaded film is improved, and the sustained-release and release-controllable effects are achieved.

The invention belongs to the technical field of agricultural chemistry, and particularly relates to a naphthoquinone compound with tobacco black shank resisting activity as well as a preparation method and application of the naphthoquinone compound. The naphthoquinone compound is prepared by the following steps: by taking honeysuckle branches as raw materials, extracting with high-concentration methanol, high-concentration acetone / water or high-concentration ethanol / water, merging extracting solutions, filtering, and concentrating under reduced pressure to obtain extract; packing the extract by using a silica gel dry method and carrying out silica gel column chromatography; carrying out gradient elution by using a chloroform-acetone solution; and further separating and purifying the 7: 3 part of the eluent by high performance liquid chromatography to obtain the required naphthoquinone compound. The invention further discloses application of the compound, and activity tests show that the compound has a good inhibiting effect on tobacco black shank. The compound disclosed by the invention is novel in structure, has relatively good black shank resisting activity, can be used as a lead compound for resisting tobacco black shank, and is used for research and development of biological pesticides for preventing and treating tobacco black shank.

The invention belongs to the technical field of high polymer material detection, and discloses a quantitative detection method for potassium perfluorobutanesulfonate in a high polymer material and application of the quantitative detection method. According to the method, trichloromethane reflux extraction and methanol precipitation are adopted as pretreatment, and specific liquid chromatography-tandem mass spectrometry detection conditions are matched, so that excellent recovery rate and extremely low limit of quantitation are obtained, and the determination requirement of potassium perfluorobutanesulfonate in a high polymer material is met. In addition, the invention further provides a detection method for simultaneously detecting the content of the potassium perfluorobutanesulfonate and the content of the polytetrafluoroethylene in the high polymer material, the content of perfluorine in a sample is detected firstly, then the content of the potassium perfluorobutanesulfonate is detected by adopting the quantitative detection method, and the content of the polytetrafluoroethylene is calculated through the content of the potassium perfluorobutanesulfonate and the content of the polytetrafluoroethylene. The method provided by the invention is simple and easy to implement, high in accuracy and good in reproducibility.

The present application provides a rhodiolide composition with a core-shell-shell structure, a preparation method thereof comprising the steps of: 1) EGCG inclusion: adding beta-cyclodextrin and EGCG into water, ultrasonic, forming an inclusion compound; 2) liposome assembly: weighing soybean phospholipid and cholesterol, dissolving in chloroform to form a uniform solution; weighing rhodiolide, dissolving in the uniform solution, rotary evaporation to remove chloroform, then adding the inclusion compound and PBS buffer into the container, hydrating, forming a liposome wrapping rhodiolide and inclusion compound; 3) shell loading: weighing collagen, mixing with chitosan solution uniformly to form a complex; adding the complex into the liposome, the complex is coated on the outer layer of the liposome, centrifugal purification, obtaining a rhodiolide composition with a core-shell-shell structure. The present application belongs to the technical field of cosmetic raw materials, and the rhodiolide composition provided has the advantages of high stability, good transdermal effect, and significant anti-aging effect.

The invention belongs to the technical field of phytochemistry, and particularly relates to a benzo [e] pyrrolo [1, 2-a] aza # imgabs0 # compound as well as a preparation method and application thereof. The preparation method comprises the following steps: fermenting cigar stem barks, drying the fermented cigar stem barks in the sun, crushing and sieving to obtain a material a; adding a first solvent into the material a for reflux extraction to obtain an extracting solution, concentrating the extracting solution, adding a second solvent for extraction, combining the extracted extraction phases, and performing vacuum concentration to obtain an extract b; carrying out silica gel column chromatography on the extract b, eluting with chloroform-acetone eluent, monitoring by TLC (Thin Layer Chromatography), and collecting the eluent; and purifying the eluent by using a sephadex column, and separating and purifying by using high performance liquid chromatography to obtain the benzo [e] pyrrolo [1, 2-a] aza # imgabs 1 # compound with tobacco black shank resisting activity. The compound is novel in structure, has relatively good black shank resistance activity, can be used as a lead compound for resisting tobacco black shank, and is used for research and development of biological pesticides for preventing and treating tobacco black shank.

The invention relates to a melamine sponge with a chitosan-wheat straw biochar coating as well as a preparation method and application of the melamine sponge. The preparation method comprises the following steps: crushing wheat straws, and pyrolyzing under an anaerobic condition to obtain biochar; the biochar is soaked in a Prussian blue dispersion liquid for modification, and Prussian blue modified biochar is obtained; dispersing the material in a chitosan acetic acid solution for reaction to form a chitosan-charcoal composite system; adding PDMS (Polydimethylsiloxane) to carry out siloxane reaction to form stable composite sol; and finally, spraying the composite sol on the surface of the pretreated melamine sponge to form the super-hydrophobic coating. Through the synergistic effect of Prussian blue, chitosan and PDMS, the hydrophobicity and oil adsorption capacity of the material are remarkably improved, the water contact angle of the obtained sponge material can reach 168 degrees, the adsorption capacities to normal hexane and chloroform respectively reach 34 g / g and 80 g / g, and the sponge material has good cycle stability and wide application prospects in the fields of oil-water separation and environmental pollution treatment.

The invention relates to the technical field of pharmaceutical analysis, in particular to a thin-layer chromatography identification method of vitality-preserving decoction and a preparation thereof. Comprising the following steps: S1, extracting a sample to be detected by using diethyl ether, and preparing a test solution; s2, preparing a cinnamaldehyde reference substance solution and a 6-gingerol reference substance solution; the method further comprises at least one of the steps S3 and S4; s3, dripping the test solution and the cinnamaldehyde reference solution on the same thin-layer plate, developing with a first developing solvent, and identifying the cinnamon; the first developing solvent comprises petroleum ether and ethyl acetate in a volume ratio of (15-20): 3; s4, dripping the test solution and the 6-gingerol reference substance solution on the same thin-layer plate, developing with a second developing solvent, and identifying the ginger; the second developing solvent is prepared from petroleum ether, trichloromethane and ethyl acetate according to the volume ratio of (2-4): (1-2): (1-3). The method is good in overall separation effect, clear in spots, good in specificity and durability, and capable of identifying the medicine flavors of cinnamon and ginger in the vitality-preserving decoction and the preparation thereof.

The invention provides a salidroside composition with a core-shell-shell structure, and a preparation method of the salidroside composition comprises the following steps: 1) EGCG (epigallocatechin gallate) clathration: adding beta-cyclodextrin and EGCG into water, and carrying out ultrasonic treatment to form a clathrate compound; 2) liposome assembly: weighing soybean phospholipid and cholesterol, and dissolving in chloroform to form a uniform solution; the preparation method comprises the following steps: weighing salidroside, dissolving the salidroside in a uniform solution, carrying out rotary evaporation to remove chloroform, then adding an inclusion compound and a PBS (Phosphate Buffer Solution) into a container, and hydrating to form lipidosome wrapping the salidroside and the inclusion compound; 3) shell loading: weighing collagen, and uniformly mixing the collagen with the chitosan solution to form a compound; the salidroside composition with the core-shell-shell structure is obtained by dropwise adding the compound into the lipidosome, coating the outer layer of the lipidosome with the compound and carrying out centrifugal purification. The invention belongs to the technical field of cosmetic raw materials, and provides a salidroside composition which has the advantages of high stability, good transdermal effect, remarkable anti-aging effect and the like.

The invention relates to the field of antibacterial functional polymer materials, and discloses a quaternary phosphonium salt type isobutyl cationic polymer as well as a preparation method and application thereof. The preparation method of the polymer comprises the following steps: under a cationic polymerization condition and in the presence of a solvent and a catalyst, isobutene and alkyl styrene are contacted for cationic polymerization, a terminator is added for terminating to obtain a solution containing an isobutene-alkyl styrene copolymer, the solution and a halogenating agent are subjected to a halogenating reaction, and the isobutene-alkyl styrene copolymer is obtained. The preparation method comprises the following steps: preparing a halogenated isobutylene-alkyl styrene copolymer solution, contacting the halogenated isobutylene-alkyl styrene copolymer solution with an organic phosphine compound, carrying out an ionization reaction to obtain a slurry material containing solid particles, carrying out solid-liquid separation to obtain a solid phase, and washing and drying the solid phase with a solvent and deionized water to obtain the quaternary phosphonium salt type isobutylene cationic polymer. The solvent is selected from dichloromethane and / or trichloromethane. The polymer prepared by the method is high in purity, high in quaternary phosphonium salt group content and higher in antibacterial property.

The invention belongs to the technical field of organic synthesis, and particularly relates to a preparation method of an olefin monomer of benzocrown ether imidazole polymerized ionic liquid. Comprising the following steps: adding benzo-12-crown-4-ether, iodo-alkyl acid and polyphosphoric acid into a heat preservation kettle, carrying out heating reaction, adding chloro-alkyl acid, carrying out heating reaction, dropwise adding water to hydrolyze residual polyphosphoric acid, extracting an organic phase by chloroform, washing chloro-alkyl acid by using a sodium carbonate aqueous solution, drying, carrying out suction filtration, and transferring into a reaction kettle; adding 1-methylimidazole, carrying out heating reaction, carrying out rotary evaporation to obtain chloroform, adding dioxane, triethylamine and hydroquinone, carrying out heating reaction, and carrying out rotary evaporation to obtain dioxane; the ionic liquid, organic salt and pure water are added into a dissolving kettle, dichloromethane is used for extracting an organic phase, pure water is used for washing chloride ions and iodide ions in the organic phase, dichloromethane is obtained through rotary evaporation, and a product is dried in vacuum. According to the method, the steps of acylation, quaternization and dehydrohalogenation are used, so that the steps are simplified, and the purification treatment process of acylation after quaternization is avoided.

The invention discloses bacillus velezensis Bve1910, which is preserved in the China Center for Type Culture Collection (CCTCC) and has the preservation number of CCTCC NO. M 20221965. The strain and metabolites thereof have strong antagonistic effects on more than ten types of marine culture animal pathogenic bacteria such as vibrio harveyi, vibrio erwiniae, vibrio canbayi, vibrio parahaemolyticus, vibrio alginolyticus, vibrio anguillarum, vibrio cholerae, vibrio shewanii, vibrio rotifer, pseudoalteromonas and photobacterium damarae, are strong in antibacterial activity and wide in antibacterial spectrum, and can be used for preparing marine culture animal pathogenic bacteria. According to the preparation method, the traditional Chinese medicine composition is simple in preparation method and rich and stable in metabolite, an ammonium sulfate precipitation extract, a hydrochloric acid precipitation methanol extraction extract, an ethyl acetate extraction extract and a chloroform extraction extract all have a broad-spectrum bacteriostatic effect, functional substances playing the broad-spectrum antagonistic bacteriostatic effect are compounded and diversified, and the directional selection effect on bacteria in the environment and ecology is not generated; and pathogenic bacteria are not easy to generate tolerance, so that the method has good application safety and ecological safety properties.

A method for separating VdF and acetylene, the method comprising: a step for mixing a first mixture containing VdF and acetylene with an extraction solvent, which is at least one compound selected from the group consisting of methanol, acetone, chloroform, and 1-chloro-2, 3, 3-trifluoro-1-propene, to obtain a mixture for extraction; and a step in which the mixture for extraction is distilled to obtain a distillate having VdF as the main component and a bottom product having an extraction solvent as the main component and further containing acetylene, respectively.

The present invention discloses a metal porphyrin containing a hexafluoropropylene trimer group, a preparation method thereof and an application; a benzaldehyde derivative containing a hexafluoropropylene trimer group, freshly distilled pyrrole, and a metal acetate are dissolved in a mixed solvent of methanol and chloroform, and under a nitrogen atmosphere, the reaction is stirred at 0 to 80 °C for 6.0 to 168.0 h. After the reaction is completed, through post-treatment, a metal porphyrin containing a hexafluoropropylene trimer group is obtained; the metal porphyrin containing a hexafluoropropylene trimer group described in the present invention can be used in the reaction of catalytic oxidation of cycloalkanes by O2. The cycloalkane oxidation method provided by the present invention has high selectivity for partial oxidation products (cycloalkyl alcohols and cycloalkyl ketones), low contents of explosive peroxides and deeply oxidized product aliphatic diacids, and is a safe, effective cycloalkane oxidation method with low separation energy consumption.

The invention relates to the technical field of power semiconductor packaging, in particular to a high-dielectric-property homogeneous polymer coating suitable for a medium-voltage SIC power module and application, the polymer coating is prepared from a solution formed by dissolving dicyclohexyl-3, 4, 3 ', 4'-tetracarboxylic dianhydride and 2, 2 '-dimethyl-[1, 1'-biphenyl]-4, 4 '-diamine in chloroform, and the polymer coating is prepared from a high-dielectric-property homogeneous polymer coating suitable for the medium-voltage SIC power module and a high-dielectric-property homogeneous polymer coating suitable for the high-dielectric-property homogeneous polymer coating suitable for the medium-voltage SIC power module. The dielectric constant of the polymer coating is 4.52 under 1kHz, the dielectric constant of the polymer coating is 3.94 under 100kHz, and the breakdown field strength of the polymer coating is 700kV / mm. The coating is verified through electric field simulation, the maximum electric field intensity in the silica gel can be reduced to 43% or below of the original maximum electric field intensity through the coating with the thickness of 80 microns, and partial discharge can be effectively restrained; meanwhile, compared with an uncoated sample, the partial discharge initial voltage (PDIV) is averagely improved by 101%, and the insulation requirement of a 15kV medium-voltage module is met.

This invention provides a method for preparing dibenzoyl-p-benzoquinone dioxime using a microchannel reactor. The method includes the following steps: 1,3-dicyclohexylcarbodiimide and a catalyst are mixed uniformly and preheated to obtain a premix; a chloroform solution of p-benzoquinone dioxime, a chloroform solution of benzoic acid, and the premix are simultaneously introduced into the microchannel reactor for reaction; the resulting reaction solution is subjected to vacuum distillation, washing, filtration, and drying to obtain dibenzoyl-p-benzoquinone dioxime. The synthesis method of this invention features high reaction conversion rate, high production efficiency, few side reactions, and high safety. The obtained dibenzoyl-p-benzoquinone dioxime product has stable quality. Furthermore, the organic solvent used can be recovered and reused after vacuum distillation, overcoming the problems of inaccurate temperature control, poor safety, numerous side reactions, and difficult waste treatment in traditional processes. This provides a new approach for the synthesis of dibenzoyl-p-benzoquinone dioxime.

The invention discloses a skin-friendly organopolysiloxane elastomer material and a preparation method thereof, and relates to the technical field of organosilicon materials. The preparation method of the skin-friendly organopolysiloxane elastomer material at least comprises the following preparation steps: dissolving a hydrophilic modified polysiloxane elastomer in chloroform, pouring into a mold, and drying to obtain a supporting layer; the aloe polysaccharide modified polysiloxane copolymer is dissolved in a tetrahydrofuran / water mixed solvent, the mixture is smeared on the supporting layer, a contact layer is formed after drying, and the skin-friendly organopolysiloxane elastomer material is obtained. The skin-friendly organopolysiloxane elastomer material prepared by the invention has good mechanical properties, air permeability and water absorption, is beneficial to growth and regeneration of wound tissues when being used as a dressing, and accelerates healing of wounds.

The invention discloses a novel acinetobacter baumannii phage vBAbaMpha0264 and application thereof, and belongs to the technical field of microorganisms. The acinetobacter baumannii phage vBAbaMpha0264 is preserved in the Guangdong Microbiological Culture Collection Center on August 28, 2025, the preservation number of the acinetobacter baumannii phage vBAbaMpha0264 is GDMCC (China General Microbiological Culture Collection Center) NO: 66893-B1, and the preservation address of the acinetobacter baumannii phage vBAbaMpha0264 is Institute of Microbiology, Academy of Sciences, Guangdong. The bacteriophage vBAbaMpha0264 has high cracking activity, environmental stability and safety, shows a breakthrough host range, and can be used for effectively cracking a plurality of acinetobacter bacteria including hospital acinetobacter and acinetobacter sysei. Meanwhile, the bacteriophage has excellent chloroform tolerance, and the stability of the bacteriophage is obviously superior to that of the existing bacteriophage. Due to the characteristics, the phage vBAbaMpha0264 overcomes the technical prejudice of'narrow spectrum and instability 'of the traditional phage, and a brand new technical scheme is provided for developing a universal antibacterial preparation covering more pathogenic bacteria.

The invention discloses a method for simultaneous thin-layer identification of ginseng and glossy privet fruit in cordyceps sinensis capsules, and belongs to the field of health food quality control. Aiming at the defects of separate detection, tedious process and low efficiency in the prior art, the method comprises the following steps: preparing a test solution through water ultrasonic extraction, water saturated n-butyl alcohol extraction and washing with a 5-8% ammonia water solution, matching with a ginseng / glossy privet fruit reference medicinal material and a mixed reference solution, and adopting a trichloromethane-methanol-underwater layer solution as a developing solvent. Synchronous identification of two components is realized through double processes of iodine fumigation sunlight inspection and sulfuric acid ethanol spray ultraviolet 365nm inspection. The method is good in separation degree, clear in spots, excellent in specificity and durability, efficient in operation and low in reagent toxicity, the detection cost is greatly reduced, reliable support is provided for cordyceps sinensis capsule quality control, and a technical normal form is provided for synchronous thin-layer identification of multiple traditional Chinese medicinal materials.

The application discloses a preparation method of a Zr-based metal-organic framework-based mixed matrix membrane, and relates to the field of gas separation membranes.The mixed matrix membrane is prepared by taking a defect-exchanged Zr-based metal-organic framework material as a filler and polyimide 6FDA-DAM as a polymer matrix.The application overcomes the defects of the prior art, and o-TBA-U iO is prepared by dispersing a defect state U iO-66 in an N,N-dimethylformamide solution of o-TBA (o-Tri-fluoromethyl benzoic acid) and then performing a heating reaction, followed by centrifugation, washing and drying in sequence.Then, the o-TBA-U iO is mixed with 6FDA-DAM in a chloroform solution, and a mixed matrix membrane is prepared by a solvent evaporation method.The application has the advantages and benefits that the MOF-based mixed matrix gas separation membrane has good C3H6 permeability and ultrahigh C3H6 / C3H8 ideal selectivity, and the main reason is that the introduction of o-Tri-fluoromethyl benzoic acid enhances the molecular sieving ability of the membrane for C3H6 / C3H8 and the preferential diffusion rate of C3H6.

The present invention relates to a composition for preventing, alleviating or treating liver cancer, containing, as an active ingredient, a Melosila nummuloides extract, and, more specifically, to an excellent cell death and metastasis inhibitory effect of a Melosila nummuloides extract on liver cancer cells, the Melosila nummuloides extract being extracted using a mixed solvent of chloroform and methanol.

The application discloses a kind of artificial cells for single-cell mass spectrometry and preparation method thereof, wherein artificial cell includes internal water phase, intermediate oil phase and external water phase;Internal water phase includes polyethylene glycol and polyvinyl alcohol aqueous solution, intermediate oil phase includes L-alpha-phosphatidylcholine chloroform and hexane mixture;External water phase includes PVA and F-68 aqueous solution.Compared with natural cell sample, the novel artificial single cell based on microfluidic self-assembly developed in the application has better uniformity, stability and controllability, effectively avoids significant measurement difference between single-cell individual samples, and effectively solves the problem of difficult stable preservation of biological samples.The proposed artificial cell preparation method will largely solve the problem of lack of standard reference material in the field of single-cell mass spectrometry, making the results of single-cell mass spectrometry method more accurate, reliable and mutually recognized.

The invention discloses a device and a process method for separating n-hexane, acetone and chloroform by applying an extractive distillation technology to a dividing wall column, and relates to the technical field of chemical processes. The device comprises two parts: one part is an extractive distillation dividing wall tower, and the other part is a solvent recovery tower. According to the technical innovation, two extractive distillation towers in a conventional three-tower extractive distillation process are coupled into one dividing wall tower by utilizing a dividing wall tower technology, so that the dividing wall tower can be used for helping to industrially separate high-purity n-hexane, acetone and chloroform, meanwhile, the use cost of equipment is reduced, the use amount of circulating water and system heat energy is reduced, and the energy consumption is greatly reduced; and as the practicability and the economic benefit are remarkable, the method has a wide application prospect.