The invention discloses a method for promoting coconut
callus induction by using
puerarin, and relates to the technical field of
plant tissue culture. According to the method, tender inflorescences of coconut trees serve as
tissue culture explants, a Y3 culture medium serves as a basic culture medium, 3 mg / L
puerarin is added in the
callus induction stage, it is found that the
puerarin can partially replace 2, 4-D or
dicamba, the use amount of the 2, 4-D or
dicamba is reduced, the risk of subsequent regeneration malformation is reduced, the
browning rate of the explants is reduced by 43%, the
callus induction rate is increased by 16%, and the
survival rate of the explants is increased. And the
induction time is shortened by 30 days. Aiming at the problems of explant
browning and low callus induction rate caused by high-concentration herbicide
plant hormones (2, 4-D or
dicamba), puerarin is added in the coconut
tissue culture regeneration process, so that the dosage of 2, 4-D or dicamba is reduced, the explant
browning rate is reduced, the callus induction rate is improved, the
induction time is shortened, and the tissue culture regeneration method has the advantages that the tissue culture regeneration efficiency is improved, and the tissue culture regeneration cost is reduced. The method has good popularization and application prospects in the field of coconut tissue culture and rapid propagation.