41 results about "Oil palm" patented technology

The invention discloses an oil palm U6 promoter gene and application thereof, and belongs to the technical field of biology. The nucleotide sequence of the promoter gene is shown as SEQ ID NO.1. The oil palm RNA polymerase III type promoter gene, namely the oil palm endogenous U6 promoter gene EgU6, is obtained by cloning in an oil palm genome for the first time, and the promoter gene has high transcriptional activity and can drive downstream fluorescent protein mNeonGreen expression. The candidate oil palm endogenous U6 promoter gene can be provided for subsequently establishing a high-efficiency oil palm gene editing technology system based on a CRISPR / cas9 (Clustered Regularly Interspaced Short Palindromic Repeats / Cas9) system.

The invention relates to a medicine for skin regeneration and repair and a preparation method thereof, and belongs to the technical field of biological medicine, the medicine comprises cyperus esculentus extract peptide, brooklet anemone root tremella extract, rhodomyrtus tomentosa root-suberect spatholobus stem-radix cyathulae composite extract, pomegranate peel polyphenol and the like. The cyperus esculentus extracted peptide is a product which is more than 1kDa and is obtained by performing enzymolysis on cyperus esculentus through cellulase, alpha-chymotrypsin and ficin protease in sequence and then performing purification through a G-50 sephadex column; the tremellodon gelatinosum tremella extract is a product which is less than 3kDa and is obtained by performing common enzymolysis on tremellodon gelatinosum tremella sporocarp through cellulase and bromelain; the rhodomyrtus tomentosa root-suberect spatholobus stem-radix cyathulae composite extract is a product obtained by performing gradient extraction on rhodomyrtus tomentosa root, suberect spatholobus stem and radix cyathulae through an ethanol water solution to obtain an extract, and performing polyamide resin column adsorption and LH-60 sephadex column purification in sequence. A good repairing system is constructed by multiple components, and wound repairing and healing are accelerated.

The invention provides an oil palm cluster recognition and positioning method, device and system and a storage medium, and the method comprises the steps: S1, obtaining a first image containing an oil palm cluster, the first image being obtained through the shooting of an RGBD camera, and the first image comprising an RGB channel image and D channel depth data; and S2, based on the first image, identifying and positioning the oil palm clusters. The method for identifying and positioning the oil palm clusters can simply, conveniently and efficiently identify and position the oil palm clusters.

The utility model discloses a cyperus esculentus lifting sieve device, which belongs to the technical field of agricultural machinery and comprises a frame. An inclined chain mechanism is mounted on the rack; the chain mechanism comprises at least two chains capable of rotating circularly, the high ends of the chains are in the rear direction, and the low ends of the chains are in the front direction. Connecting lugs which are uniformly distributed at intervals are arranged on the chain; each chain plate comprises a bottom plate and right-angle plates fixedly connected to the two ends of the bottom plate, and the two right-angle plates of each chain plate are detachably connected with the connecting lugs on the two chains through bolt assemblies. A push plate part bent upwards is fixedly arranged at the front end of the bottom plate, a baffle part bent downwards is arranged at the rear end of the bottom plate, oblong holes which are densely distributed are formed in the surface of the bottom plate, and the width of the oblong holes is smaller than the diameter of cyperus esculentus. According to the cyperus esculentus harvester, the chain plate is arranged to serve as a conveying device for conveying a bean-soil mixture, soil block particles are efficiently screened while the bean-soil mixture is lifted, the cyperus esculentus harvester has the effects of being reliable in work, low in cost, convenient to maintain and capable of preventing blockage, and then the cyperus esculentus harvesting efficiency is improved.

To provide laminated lumber much harder and stronger even when using a trunk of oil palm, and a manufacturing method of the laminated lumber.SOLUTION: Laminated lumber S is formed by compressing laminated bodies L made through laminating a plurality of single plates T of oil palm, wherein bacteria cellulose is impregnated in each of the single plates T. A manufacturing method is provided with: a lumbering process (1) of acquiring a single plate through lumbering a trunk P of the oil palm; a pre-treatment drying process (2) of drying the single plate T; an impregnating process (3) of impregnating the single plate T with impregnating solution including the bacteria cellulose; a crimping process (4) of compressing the laminated body L obtained by laminating the plurality of single plates T for a required time using a press machine; and a finish drying process (5) of drying thereafter. Also, it is provided with a bacteria cellulose production process (A) of producing bacteria cellulose from chips Pa of the trunk P of the oil palm including surrounding materials acquired in the lumbering process.SELECTED DRAWING: Figure 2

The invention relates to the technical field of agricultural product deep processing and functional food science, in particular to enzymolysis cyperus esculentus powder and a preparation method and application thereof. The preparation method comprises the steps that cyperus esculentus powder and water are mixed, pulped and cured; performing high-speed shearing pretreatment on the slurry; adding a compound enzyme preparation containing amylase, cellulase and protease for enzymolysis; carrying out enzyme deactivation after enzymolysis, and filtering; performing emulsification and two-stage high-pressure homogenization treatment on the filtrate; and finally spray-drying to obtain a finished product. Through the synergistic effect of high-speed shearing and composite enzymolysis, cell walls of cyperus esculentus are effectively broken, macromolecular nutrient substances are converted into micromolecules, and the solubility, taste and nutrition utilization rate of the product are remarkably improved. The prepared enzymolysis cyperus esculentus powder is good in instant solubility, good in flavor and high in stability, and can be widely applied to the fields of solid beverages, special dietary foods and the like.

The invention belongs to the technical field of oil palm fruit picking equipment, and particularly discloses an oil palm fruit picking device and a picking mechanical arm.The oil palm fruit picking device comprises a shoveling and cutting unit used for shoveling and cutting leaf stalks outside oil palm fruits and fruit stalks of the oil palm fruits, and the shoveling and cutting unit comprises a first driving mechanism and a shovel blade; the first driving mechanism is used for driving the scraper knife to linearly move for shoveling; the clamping unit is used for clamping the cut oil palm fruits, the clamping unit comprises a second driving mechanism and two clamping jaws arranged in the horizontal direction, the second driving mechanism is used for driving the two clamping jaws to open or hold tightly, and the shoveling and cutting unit is located below the two clamping jaws; the shoveling and cutting unit and the clamping unit are arranged on the fixing frame, and the two clamping jaws are located outside one end of the fixing frame. The oil palm fruit picking device is convenient and fast to operate, reduces manual intervention, improves efficiency, is high in stability and reliability, and can better meet the picking requirements of oil palm fruits.

This invention provides a method for transient transformation of oil palm zygote embryos based on Agrobacterium-mediated transformation, which employs the Agrobacterium-mediated approach and is based on the optimal Agrobacterium concentration (OD) for stable transformation of oil palm. 600 The optimal Agrobacterium infection time for zygotic embryos was determined by measuring the concentration of acetylsuccinone and co-culture time. The transient GUS expression staining rate was compared between zygotic embryos of different oil palm varieties and at different growth stages. The method described in this invention optimizes infection conditions based on stable transformation parameters, clarifies the optimal explant materials and treatment process, and effectively improves transient transformation efficiency and experimental repeatability. This study establishes for the first time an Agrobacterium-mediated transient transformation system for oil palm zygotic embryos and obtains zygotic embryos most suitable for transient transformation, achieving efficient transient expression of exogenous genes in oil palm zygotic embryos. This provides a rapid and low-cost technical means for studying oil palm gene function.

The present application relates to an oil palm UBQ promoter and application thereof, and belongs to the technical field of biology.The nucleic acid sequence of the promoter is shown as SEQ ID NO.1, the present application firstly aligns and intercepts a sequence of 2000bp upstream of a start codon in an oil palm genome, and synthesizes an oil palm RNA polymerase II type promoter, i.e., an oil palm endogenous ubiquitin promoter EgUBQ.The promoter has transcription activity, and can drive expression of downstream green fluorescent protein NeonGreen.The present application provides an oil palm endogenous UBQ promoter for genetic transformation and gene editing of oil palm.

The application provides a method for Agrobacterium-mediated genetic transformation of oil palm, and the method uses embryogenic callus of oil palm as explants, adopts Agrobacterium-mediated mode, and selects the most suitable Agrobacterium liquid concentration, immersion time, co-culture time, acetosyringone concentration and antibiotic concentration in the transformation process. An expression cassette containing an EPSPS mutant gene is used as a screening marker, and glyphosate is used as a screening agent to select glyphosate-resistant callus. The screening efficiency of the application is higher than that of the traditional hpt / hygromycin screening system. Meanwhile, the effectiveness of the EPSPS / glyphosate screening system in the genetic transformation of oil palm is proved, which marks the first application of the system in the genetic transformation of oil palm and provides a valuable tool for promoting the research of the important economic crop.

The application discloses a hydroxyl functional basic ionic liquid and a preparation method thereof, and a method for preparing biodiesel from cyperus oil, and relates to the field of biodiesel preparation methods.The chemical structure of the hydroxyl functional basic ionic liquid is as follows: wherein R is OH, imidazole or morpholine, and the method for preparing the biodiesel is as follows: taking cyperus oil and a short-chain alcohol as raw materials, taking the hydroxyl functional basic ionic liquid as a catalyst, and preparing the biodiesel through an ester exchange reaction.After the reaction is completed, the novel hydroxyl functional basic ionic liquid and the product are automatically separated.The hydroxyl functional basic ionic liquid catalyzes the preparation of the biodiesel, and the reaction process is simple, the product is easy to separate, the yield is high, no pollution is caused, the method is friendly to the environment, the prepared biodiesel has few impurities, the purity is high, and the yield of the biodiesel is as high as 98%.

The invention discloses a mcl-PHA production method based on a wood and agricultural waste composite carbon source, and relates to the technical field of bio-manufacturing, the method comprises the following steps: adopting Pseudomonas putida BWCC66157 to carry out fermentation in an improved M9 minimum culture medium; a lignin-derived carbon source (such as vanillic acid) is used as a basic substrate; respectively introducing rubber wood hydrolysate and oil palm pomace palm oil as co-substrates; the directional regulation and control of the composition of the mcl-PHA monomer are realized by regulating and controlling the ratio of the co-substrate; and extracting the PHA by adopting a conventional method and detecting the monomer composition of the PHA. The PHA yield can be increased, new monomer components can be obtained, high-valued utilization of wood carbon sources and agricultural waste is achieved, and a new technical approach is provided for production of low-cost and degradable biological materials.

The application discloses a method for promoting oil palm callus proliferation and somatic embryo induction by using baicalin. The tender yellow leaf of oil palm is used as a tissue culture explant, and Y3 medium is used as a basic medium, and baicalin is added to promote the induction and proliferation of oil palm callus and somatic embryo generation. In the callus induction and proliferation stage, 0.5 mg / L of baicalin can replace part of the effect of 2,4-D or dicamba, reduce the dosage of 2,4-D or dicamba, reduce the risk of subsequent regeneration deformity, shorten the callus induction time by 20 days, and increase the callus proliferation rate by 48.19%; in the somatic embryo induction stage, 0.3 mg / L of baicalin can completely replace the plant hormone 6-BA, and the somatic embryo induction time is shortened by 31 days, and the somatic embryo induction rate is increased by 5%. Therefore, the addition of baicalin in the medium can improve the efficiency of oil palm callus proliferation and somatic embryo induction, shorten the induction time, and has a good popularization and application prospect in the field of oil palm tissue culture and rapid propagation.

The present application belongs to the field of feed fermentation, and particularly relates to a concentrated-flavor cyperus esculentus dry grass feed based on biosynthesis and a preparation method thereof. The concentrated-flavor cyperus esculentus dry grass feed comprises cyperus dry grass, a bacteria liquid of fused weissella and carbon source and nitrogen source. The fused weissella has been preserved in the China General Microbiological Culture Collection Center (CGMCC) with a preservation number of CGMCC No. 24053. The technical solution of the present application utilizes biotechnology to assist in fermenting cyperus esculentus, generates organic acid by decomposing fiber and utilizing soluble carbohydrate and other fermentation substrates to inhibit the growth of mold and other spoilage bacteria and reduce the loss of nutritional ingredients. Meanwhile, flavor substances (mainly including alcohol, aldehyde, phenol, acid, ester, ketone and hydrocarbon substances) are greatly enriched after fermentation, which can effectively increase the aroma components of the feed.

The invention discloses a camellia oil decolorizing method based on modified palm wood-based activated carbon, and belongs to the technical field of grease refining. According to the method, renewable palm wood (oil palm) is used as a raw material for preparing activated carbon, OPAC (oil palm wood-based activated carbon) is prepared, modification treatment is used for increasing the high specific surface area of the activated carbon with rich pore structures so as to increase the adsorption capacity of the activated carbon, the modified palm wood-based activated carbon is applied to the camellia oil decolorizing process, and the camellia oil decolorizing effect is improved. The method can effectively remove carotenoid, chlorophyll and impurities in the camellia oil, retains active components in the camellia oil, has the advantages of high decolorization efficiency, reusability of activated carbon, low cost, environmental friendliness and the like, and is suitable for industrial production and application.

The application discloses a primer composition for identifying or assisting in identifying a plant protein raw material source and application thereof. The primer composition for identifying or assisting in identifying the plant protein raw material source comprises seven primer pairs in independent packages, and the names of the seven primer pairs are E1, E2, E3, E4, E5, E6 and E7. Seven primers are designed for specific genes of rapeseed, peanut, sunflower seed, oil palm, soybean, sesame and cotton seed of the plant protein raw material source, detection is carried out through a polymerase chain reaction (PCR) method, and the primers have strong specificity after verification, and can be used for identifying the types of plant source feed ingredients in feed.

The utility model relates to oil palm plant technology field especially is a kind of oil palm planting soil breaking device, including pedestal, vibration subassembly, brush cleaning subassembly, conveying component and object carrying assembly, in the utility model, through the limit shell, soft brush, conveying belt, mounting block, mounting shaft, rotating seat and object carrying plate being set, the device can be driven by the limit shell synchronous vibration of anti-falling shell and rotating shaft through vibration, through the movable conveying belt drive multiple pairs of mounting block to move, and then make object carrying plate one side drive oil palm seed grain to move one side vibration, simultaneously through the cooperation of rotating seat and mounting shaft make object carrying plate can occur random rotation, make the soil and rhizome on oil palm seed grain rapidly vibrate and break, again through soft brush to oil palm seed grain is swept, the soil and rhizome on oil palm seed grain can be more thoroughly removed, so the device has better screening effect and higher screening efficiency.

The present application belongs to the technical field of pesticides, and particularly relates to a fungicidal composition for preventing and treating oil olive root rot. The effective components of the fungicidal composition are formed by binary compounding of fluoximide and berberine or natamycin. When fluoximide and berberine or natamycin are binary compounded, the synergistic coefficient of the fungicidal composition to phytophthora cinnamomi is greater than 1.5, that is, the fungicidal composition exhibits a certain synergistic effect. In particular, when the mass ratio of fluoximide to natamycin is 8:1, the SR value of the fungicidal composition reaches 11.2394, and the synergistic effect is the most significant. Therefore, the fungicidal composition can help to improve the prevention and treatment effect on oil olive root rot, and can be used for developing a high-efficiency fungicide for preventing and treating oil olive root rot. In addition, the fungicidal composition is formed by binary compounding of two effective components, can delay the development of pathogen resistance, and reduce the risk of drug resistance.

This invention relates to a groundwater level indicator stick used to monitor the groundwater level in water management activities on peatlands. This invention is intended to overcome the limitations of conventional measuring sticks that use fixed reference points so that the measurement results can be less representative due to changes in peat surface elevation caused by subsidence, compaction, decomposition of organic materials, or changes in hydrological conditions. The groundwater level indicator stick, according to this invention, consists of a stick equipped with a measuring scale, a support part installed on a relatively stable soil layer, an adaptive peat surface reference system that adjusts the reference position to changes in peat surface elevation, and an indicator for reading the water level against the actual peat surface.With this reference system, measurement results remain aligned with the actual conditions of the peat surface, thus improving the accuracy, consistency, and reliability of long-term water level monitoring. This invention is easy to install, requires no electrical power source, has a simple construction with low maintenance costs, and can be applied to oil palm plantations, peat conservation areas, peat ecosystem rehabilitation, hydrological research, and other environmental monitoring activities.

The invention belongs to the technical field of forestry operation, and particularly provides a method and device for automatically picking oil palm clusters through picking equipment, and the method comprises the steps that the picking equipment identifies and positions all oil palm trees in a target operation area; the picking equipment determines a picking path according to the positions of all the oil palm trees, and the picking path sequentially passes through all the target fruit trees; the picking device moves according to the picking path, when the picking device moves to the position where one oil palm tree is located, the picking device picks mature oil palm clusters on the oil palm tree, and after picking is finished, the picking device moves to the next oil palm tree for picking till the last oil palm tree on the picking path is picked. According to the preparation method provided by the embodiment of the invention, the picking equipment can realize navigation in an oil palm forest by accurately identifying and positioning the oil palm trees; in addition, automatic picking is achieved through the picking equipment, manpower resources are greatly saved, and automatic management of the oil palm forest is achieved.

ABSTRACT A MACHINE FOR PROCESSING OIL PALM PRODUCTS The present invention relates to a machine for processing oil palm products. More specifically, it relates to a machine for cutting, crushing, grinding, finely crushing, or shredding oil palm products, such as oil palm fruits in the form of fresh fruit bunches.The machine comprises a motor (100); a gearbox (200) connected to the motor (100); a coupling means (300) connected to the gearbox (200); a bearing system (400) connected to the coupling means (300); and a processing means (500) connected to the bearing system (400), wherein the processing means (500) further comprises an inlet (501) for receiving oil palm products; at least one outlet (502) for dispensing processed oil palm products; at least one housing (503) further comprising a horizontal cylindrical bore (504); and a worm gear connected to the bearing system (400), which is horizontally located along the center of the processing means (500) and extends along the entire length of the processing means (500). Furthermore, the surface of the horizontal hole (504) of the housing (503) comprises at least one threaded element (508).Drawing accompanying the summary: Figure 1.

The invention discloses a culture medium for inducing test tube flowering of oil palm. The culture medium comprises a pre-culture medium and an induction culture medium. The invention also discloses a method for inducing the flowering of the oil palm in the test tube. The method comprises the following steps: acquiring the clumpy buds of the oil palm, pre-culturing the clumpy buds of the oil palm, and inducing and culturing the clumpy buds of the oil palm. The invention develops a specific, efficient and reproducible test tube flowering method aiming at the oil palm, the natural flower bud development cycle (2-3 years) can be shortened by more than 85%, the influence of the external environment on the reproductive growth of the oil palm is overcome, a controllable model is provided for the research on the reproductive development mechanism of the oil palm, and meanwhile, the technical support capability of the oil palm planting in the tropical northern region is improved; the method has remarkable scientific research and application values.

The invention belongs to the technical field of oil palm fruit picking, and particularly discloses automatic oil palm fruit picking equipment which comprises a walking chassis; a lifting device; the picking device is used for picking oil palm fruits, and the lifting device is used for lifting the picking device; the picking device comprises a shoveling and cutting unit and a clamping unit, the shoveling and cutting unit is used for shoveling and cutting leaf stalks outside the oil palm fruits and fruit stalks of the oil palm fruits, and the clamping unit is used for clamping the cut oil palm fruits; the shoveling and cutting unit comprises a first driving mechanism and a shovel blade, and the first driving mechanism is used for driving the shovel blade to linearly move for shoveling and cutting; the clamping unit comprises a second driving mechanism and two clamping jaws arranged in the horizontal direction, the second driving mechanism is used for driving the two clamping jaws to be opened or held tightly, and the shoveling and cutting unit is located below the two clamping jaws. The oil palm fruit picking device can achieve automatic picking of oil palm fruits, operation is convenient and fast, manual intervention is reduced, efficiency is improved, and stability and reliability are high.

The invention relates to the technical field of agricultural product picking, and discloses a sleeve type oil palm fruit picking and cutting device with a gravity buffering function. After cutting, the weight detection piece measures the weight of the fruit bunches in real time and feeds back the weight to the buffer; the buffer dynamically adjusts the current of the electromagnetic coil and accurately controls the magnetic damping of the buffer plate; for heavy fruit bunches, current and damping force are increased, impact energy is effectively absorbed, and hard collision and fruit breakage are prevented; for light fruit bunches, damping force is reduced, excessive buffering or rebounding is avoided, and soft material receiving is achieved; the problem that traditional fixed damping is easy to reach the limit or the impact is too hard is solved through self-adaptive buffering which is different from objects, and the buffering reliability and the fruit integrity are greatly improved; meanwhile, a smooth falling channel is formed by the through groove, the guide cylinder and the semicircular material receiving guide plate, and it is ensured that the fruit bunches are accurately guided to a buffer area.

The invention discloses a molecular marker method for identifying green fruit type and black fruit type oil palm. The molecular marker is one or more of SNP1 locus and SNP10 locus. The core SNP marker of the oil palm is developed on the basis of gene analysis and a KASP technology, 10 SNP markers with high specificity and high sensitivity are finally screened out, and green fruit type and black fruit type oil palm varieties can be rapidly, accurately and effectively identified by utilizing the molecular marker. According to the method, the variety purity and variety type can be identified in the oil palm seedling stage, the breeding period is shortened, and a resource basis is provided for oil palm variety cultivation and product development in the future.

This invention discloses a culture medium for inducing in vitro flowering of oil palm, comprising a pre-culture medium and an induction culture medium. The invention also discloses a method for inducing in vitro flowering of oil palm, comprising steps such as obtaining oil palm shoot clusters, pre-culturing oil palm shoot clusters, and inducing oil palm shoot cluster culture. This invention develops a specific, efficient, and reproducible in vitro flowering method for oil palm, which can shorten the natural flower bud development cycle (2-3 years) by more than 85%, overcome the influence of the external environment on the reproductive growth of oil palm, provide a controllable model for the study of the reproductive development mechanism of oil palm, and simultaneously enhance the technical support capacity for oil palm cultivation in the northern edge of the tropics, possessing significant scientific research and application value.

This invention relates to the process of producing hydrogel film from carboxymethyl cellulose from OPEFB which includes the formula and method of hydrogel film process. Hydrogel film is synthesized using 4% CMC solution; CMC is dissolved in water then added 5% glycerol from the volume of 4% CMC solution; added 2.5% citric acid and 2.5% acetic acid from the volume of 4% CMC solution. The mixed solution is stirred at room temperature for 2 hours; then cast and heated at 60°C for 24 hours. The formed hydrogel film is used as an absorbent pad component to absorb drip loss of fresh meat; fresh fish / chicken fillets and maintain humidity in storage at cold temperatures.

The invention discloses a method for promoting oil palm callus proliferation and somatic embryo induction by utilizing baicalin. Oil palm tender yellow leaves are used as tissue culture explants, a Y3 culture medium is used as a basic culture medium, and baicalin is added to promote oil palm callus induced proliferation and somatic embryogenesis. Wherein 0.5 mg / L of baicalin is added in the callus induction proliferation stage, the effect of part of 2, 4-D or dicamba can be replaced, the dosage of 2, 4-D or dicamba is reduced, the risk of subsequent regeneration malformation is reduced, the callus induction time is shortened by 20 days, and the callus proliferation rate is increased by 48.19%; 0.3 mg / L of baicalin is added in the somatic embryo induction stage, so that plant hormone 6-BA can be completely replaced, meanwhile, the somatic embryo induction time is shortened by 31 days, and the somatic embryo induction rate is increased by 5%. Therefore, by adding the baicalin into the culture medium, the callus proliferation and somatic embryo induction efficiency of the oil palm can be improved, the induction time is shortened, and the method has good popularization and application prospects in the field of tissue culture and rapid propagation of the oil palm.

1. Name of the designed product: Oil palm fruit sickle adaptor. 2. Use of the designed product: The designed product is used for fixing and adapting the sickle. 3. Design points of the designed product: In shape. 4. Picture or photograph best indicating the design points: Fig. 1.

The present application relates to a kind of skin regeneration repair medicine and preparation method thereof, belong to biological medicine technical field, and medicine includes oil palm extraction peptide, tiger palm silver ear extract, Gangdeng root-milletia nitida-Siberian milkvetch composite extract and pomegranate peel polyphenol etc.Oil palm extraction peptide is oil palm sequentially by cellulase, alpha-chymotrypsin and ficin enzymolysis, then by G-50 dextran gel column purification and obtain the product above 1kDa;Tiger palm silver ear extract is tiger palm silver ear fruit body by cellulase and bromelain common enzymolysis, and obtain the product below 3kDa;Gangdeng root-milletia nitida-Siberian milkvetch composite extract is Gangdeng root, milletia nitida and siberian milkvetch by gradient extraction of ethanol aqueous solution and obtain extract, sequentially by polyamide resin column adsorption and LH-60 dextran gel column purification and obtain the product.Multiple components construct good repair system, accelerate wound repair healing.