295 results about "Microbiological growth" patented technology

The invention relates to the technical field of microbiological detection and image detection, and provides a microwell plate microbiological growth state detection method, system and equipment based on artificial intelligence. The system comprises an image acquisition module, an image segmentation module, a data set construction module, an image data enhancement module, an image classification module, a classification result determination module and a reasoning module, and is used for detecting whether bacteria grow in the microwell plate; or the system comprises an image regression module and a regression error evaluation module and is used for detecting the biomass of the thalli. According to the invention, a self-built photographing device can be adopted to realize on-site direct automatic detection without sampling, so that high-throughput automatic accurate analysis of the growth state of microorganisms in the microwell plate can be conveniently and quickly realized, experiment consumables and biological samples can be saved, the contamination risk can be reduced, and the experiment efficiency can be remarkably improved. Therefore, the working intensity of detection personnel is reduced, and the method has wide application value. Meanwhile, the complexity of detecting the growth state of the microorganisms in the pore plate is reduced, and popularization and application are facilitated.

This invention discloses a soil improvement and fertilization fertilizer for rhizosphere micro-domains in saline-alkali land and its preparation method, belonging to the field of saline-alkali land management technology. The fertilizer consists of a microbial core, a slow-release fertilizer sub-outer layer, and a soil conditioner outer layer. The microbial core is composed of a compound microbial agent (composed of nitrogen-fixing bacteria, phosphate-solubilizing bacteria, and potassium-active bacteria), biochar, and a high-molecular organic membrane material. The slow-release fertilizer sub-outer layer is composed of chemical fertilizers, inhibitors (nitrification inhibitors and / or urease inhibitors), and amino acids. The soil conditioner outer layer is composed of a soil conditioner specifically for saline-alkali land improvement. The advantages of this invention are: (1) it first adjusts the soil pH and improves the soil structure, so that the microbial community can better adapt to the soil environment of saline-alkali land; (2) the biochar and the isolation membrane effectively protect the microbial activity, and release the microorganisms when the soil pH and base ions around the rhizosphere reach a low level, overcoming the saline-alkali barrier to microbial growth.

Described herein are methods and compositions for using microbial agents (probiotics) and agents that promote growth of certain microbes (prebiotics) for management (including prevention and treatment) of musculoskeletal disorders, including osteoporosis, osteopenia, Paget's disease, stunting, osteoarthritis, osteomyelitis, and delayed or non-union fractures. Also described herein are methods and compositions for using probiotics and prebiotics for management of inflammation, and symptoms of menopause.

The invention discloses an activated sludge self-adaptive regulation and control method based on multi-source data mining, and belongs to the technical field of sludge treatment.The activated sludge self-adaptive regulation and control method comprises the steps that a microflora feature data set is obtained from a sewage treatment system; according to the microflora feature data set, analyzing the sludge state by adopting a classification model, and judging the growth stage of microorganisms; determining the current sludge discharge load level; if the current sludge discharge load level exceeds a preset threshold value, deducing future sludge state evolution by adopting a prediction model according to the type of the current microbial growth stage and the microbial community characteristic data set to obtain an aeration intensity adjustment threshold value; determining sludge discharge configuration parameters by matching the aeration intensity adjustment threshold value with the current aeration intensity; and taking the aeration intensity adjustment threshold value and the sludge discharge configuration parameter as a sewage treatment parameter configuration scheme. The activated sludge self-adaptive regulation and control method based on multi-source data mining solves the problem that regulation and control of activated sludge are difficult to realize accurately at present.

The invention relates to the technical field of food processing, in particular to a chicken quality preservation treatment method based on sectional regulation, which is characterized in that chicken is pretreated by a composite biological preservative solution, and ice storage treatment and staged storage are combined, so that microbial growth and enzymatic activity of the chicken are effectively inhibited; fat oxidation and protein degradation are delayed, so that the chicken still keeps excellent quality in long-term storage; in the staged storage process, key indexes are monitored in real time or periodically, a threshold value comparison and stage switching mechanism is established, and accurate dynamic regulation and control of the staged modified atmosphere packaging environment under the condition of dynamic deterioration of the chicken quality are achieved. Through segmented regulation and control, juice loss, color deterioration and decay of the chicken in the storage process can be effectively prevented, so that the sensory quality and the nutritional quality of the chicken are maintained, and meanwhile, the gas utilization efficiency in the storage environment is also remarkably improved.

The invention discloses an aquatic product cold chain transportation monitoring system which is characterized in that the system comprises a hardware layer and a software layer, the hardware layer comprises a sensing control module, a structure bearing module and a communication interaction module, and the software layer comprises a data management module, an intelligent calculation module and a business application module; the sensing control module comprises an environment sensing sub-module and an equipment control sub-module, the structure bearing module comprises a compartment main body sub-module and a cargo protection sub-module, and the communication interaction module comprises an edge calculation sub-module and a vehicle-mounted interaction sub-module. According to the application, a real-time monitoring-dynamic prediction-intelligent regulation and control microbial inhibition system is constructed, a high-precision sensor is used for collecting environmental data in real time, and an LSTM-Attention model is combined for accurately pre-judging the growth trend of microorganisms, so that the effects of early warning before the colony count reaches a dangerous threshold value and automatically coordinating equipment to start targeted bacteriostatic measures are achieved, and the safety of the system is improved. And the loss rate of aquatic products caused by microbial pollution is reduced.

This invention discloses a multi-scale prediction method and system for the maximum growth rate of soil microorganisms based on genomic features, involving the interdisciplinary fields of microbial ecology and genomics. The method includes: genomic data preparation, core genomic feature extraction, single-scale growth rate prediction, community-scale extrapolation, and result verification and output. The system includes a genomic data processing unit, a core feature extraction module, a growth rate prediction model, a community-scale extrapolation module, and a result visualization and verification unit. This invention overcomes the limitations of traditional culture methods and in-situ measurement methods, achieving high-throughput and accurate prediction of the growth rates of culturable and uncultured microorganisms, and solving the problems of low coverage, fragmented scales, and high costs of existing technologies. It is applicable to soil microbial function assessment, carbon cycle model parameterization, and ecosystem management.

The invention provides a phosphorus release improving type compound fertilizer based on a fungal extracellular polymer, a preparation method and application, and belongs to the technical field of microorganisms, the phosphorus release improving type compound fertilizer is prepared by compounding the extracellular polymer generated by specific rhodotorula mucilaginosa WTSX01 and inorganic phosphate according to the mass ratio of 1: 9; the preparation method comprises the following steps: extracting and purifying high-activity EPS from a strain fermentation broth by a low-temperature dialysis method, separating lipid from EPS as a natural adhesive by a cosolvent extraction method, and finally mixing EPS, lipid and inorganic phosphate. The invention also discloses a synergistic application of the fertilizer and a phosphorus-solubilizing fungus aspergillus niger (CCTCC No. M2023240), so that the release efficiency of phosphorus in soil is improved; the fertilizer provided by the invention provides exclusive nutrition for phosphate-solubilizing microorganisms, effectively overcomes indigenous microorganism competition, remarkably promotes microorganism growth and phosphorus release, and realizes reduction and synergism of phosphate fertilizer and yield increase of crops.

The application discloses a kind of light-assisted anticorrosion coating materials and preparation method based on metal coordination structure, the light-assisted anticorrosion coating material is on the basis of traditional anticorrosion metal zinc layer continue to be coordinated with the metal coordination polymer Co (bpy) (dhbq) (bq) with photoelectric conversion capability, and form coordination complex Co (bpy) (dhbq) (bq) @Zn.Because metal coordination polymer Co (bpy) (dhbq) (bq) itself can additionally generate photo-generated electron and photo-generated current after being illuminated, provide redundant charge for Zn layer, make it be in lower potential, strengthen the function of cathodic protection.Corrosion can be inhibited through this mechanism, prolong the service life of Zn layer, provide efficient, green anticorrosion protection for the steel material that needs to be protected.In addition, metal coordination light-assisted anticorrosion technology can also inhibit marine bioattachment by slowly releasing metal ions Co, prevent biofilm formation, reduce microbial growth, prolong the service life of protected material in marine environment, also applicable to underwater bioinduced corrosion and the protection of fouling metal structure.

The utility model discloses a gas circulation type meat product fermentation device, which belongs to the technical field of flavor meat product processing, and comprises a fermentation part, a gas circulation part and a gas circulation part, the air supply part is communicated with the side surface of the fermentation part and is used for introducing air into the fermentation part; the flow guide part is communicated with one end of the air supply part so as to uniformly introduce air at a preset temperature into the fermentation part; the air return part is arranged between the fermentation part and the flow guide part in a communicating manner and realizes air flowing through the driving part; a three-way pipe valve is also arranged on the air return part, and the three-way pipe valve is communicated with an external pipeline so as to introduce fresh air or circulating air into the fermentation part. According to the fermentation device, traditional mechanical stirring is replaced with gas flow, mass transfer and heat transfer are achieved through gas circulation, growth and metabolism of microorganisms are promoted, and therefore the characteristics of meat products are converted, the flavor is improved, and the shelf life is prolonged.

A method for controlling microbial growth in a sugar processing system is disclosed, wherein the method comprises adding a peroxy acid into water of a flume system used for transporting a sugar-containing plant material from a delivery or storage location to a wash system. In one non-limiting example embodiment, the sugar-containing plant material comprises sugar beets, and the peroxy acid comprises peracetic acid.

The invention discloses an intelligent distribution scheduling method and device for egg products and a medium, and relates to the technical field of intelligent scheduling, and the method comprises the steps: collecting biological characteristic parameters of target egg products in real time, carrying out the feature encryption processing through an edge calculation node to form private data packets, receiving the private data packets of all suppliers through a message queue service, and sending the private data packets to a server; calling a homomorphic encryption library to execute weighted average aggregation on the encrypted feature vectors; and loading real-time road condition data, compartment temperature and humidity distribution and a vibration spectrum in a digital twin sandbox based on a global egg rheology knowledge graph and a microbial growth kinetic model, and coupling to solve a product risk value of a protein shear stress accumulation equation and a flora growth equation. By constructing a global egg product rheology knowledge graph and a microbial growth kinetic model, the time-space coupling characteristics of biomechanical characteristics and microbial activity of egg products in transportation are accurately captured, and the quality risk quantification precision and the transportation strategy reliability are improved.

The invention relates to a microbial growth activation accelerant based on rare earth elements as well as a preparation method and application thereof, and belongs to the field of resources and environments. The microbial growth activation accelerant based on the rare earth elements is prepared from the following components: 1 to 150g of a rare earth compound, 0.2 to 2g of pantothenic acid, 0.2 to 2g of nicotinic acid, 0.02 to 0.1 g of nicotinamide and 1000g of deionized water, the rare earth compound is one or combination of more of chlorides of lanthanum, cerium, praseodymium, neodymium, promethium, samarium, europium, gadolinium, terbium, dysprosium, holmium, erbium, thulium, ytterbium, lutetium, scandium and yttrium, nitric acid compounds and sulfuric acid compounds. A large number of cheap light rare earth elements are used as raw materials of the microbial accelerator, a new utilization approach is provided for the light rare earth elements, the light rare earth elements are converted into high-added-value products, pollution of the light rare earth elements to the environment is reduced, and meanwhile new elements are introduced into the field of microbial accelerators. More importantly, the preparation method of the microbial growth activation accelerant based on the rare earth elements provided by the invention is simple in production process and convenient for large-scale production.

To provide a method for detecting the growth of microorganisms that can accurately detect a droplet in which microorganisms have grown, to provide a method for obtaining microorganisms, to provide a kit for detecting the growth of microorganisms, to provide a kit for obtaining microorganisms, and to provide use of dye as microbial growth reporter.SOLUTION: A method for detecting the growth of microorganisms comprises: producing a droplet containing a dye whose fluorescence intensity changes by interacting with a microorganism and a membrane component possessed by the microorganism, in W / O emulsion; culturing the microorganism within the droplet; and detecting the growth of the microorganism based on the fluorescence intensity of the dye.SELECTED DRAWING: Figure 1

This invention relates to a resin-based nano-iron oxide-enhanced microbial preparation, a method for preparing the same, and its applications. In this invention, after mixing resin-based nano-iron oxide, the microbial preparation, and food waste, at room temperature, the resin-based nano-iron oxide enhances the decomposition of food waste by the microbial preparation through multiple actions, including promoting microbial growth, inducing high activity of functional proteases, and accelerating electron transport processes. The alternating aerobic and anaerobic environments allow the microbial preparation to exert its full effect, resulting in high biochemical activity of metabolites. A reaction under room temperature conditions of 3 to 5 hours can achieve a food waste reduction rate of 80% to 99%. This invention is technically simple, consumes little energy, does not cause secondary pollution, and effectively achieves the goals of food waste reduction, resource recovery, and detoxification.

The application discloses a preservative for reducing the content of polycyclic aromatic hydrocarbons in meat products and application thereof. The preservative comprises Lactobacillus pentosus R3, the Lactobacillus pentosus R3 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No. 26346, and the preservation date is December 27, 2022. The preservative is inoculated on the surface of the meat product, the growth of spoilage microorganisms can be inhibited, fat oxidation of the meat product is delayed, the shelf life of the smoked meat product is prolonged, the content of polycyclic aromatic hydrocarbons in the smoked meat product can be significantly reduced, and the food safety is improved.

The invention discloses a microorganism rotary culture device, and relates to the technical field of microorganism culture equipment, the microorganism rotary culture device comprises a main body frame, a test tube and an underframe, a rotating mechanism for driving the test tube to perform rotary culture is rotatably arranged in the main body frame; a plurality of groups of radial moving mechanisms for clamping the test tubes and driving the test tubes to move along the radial direction of the rotating mechanism are assembled on the rotating mechanism. Through cooperative work of the rotating mechanism, the radial moving mechanism, the conductive slip ring mechanism and the driving mechanism, differential rotating culture time of multiple groups of test tubes can be preset, and when a certain group of test tubes reaches culture time, a power motor of the corresponding radial moving mechanism drives a lead screw to drive the test tubes to rotate. The movable frame and the test tubes are driven to move to the center position of the rotating mechanism along the radial sliding grooves, at the moment, the test tubes only rotate along with the rotating mechanism, a worker can safely take the tubes without stopping the rotating mechanism, continuous revolution and rotation culture of other test tubes is guaranteed, and the problems of microorganism growth environment interruption and metabolic disorder caused by shutdown tube taking of a traditional device are solved.

The invention relates to a functional drinking water preparation method based on a pH response type microcapsule slow release system, and belongs to the technical field of drinking water processing. The functional drinking water is prepared from the following materials: 50-65 parts of base water, 3-6 parts of hydrolyzed protein peptide melt, 5-12 parts of transparent gel, 5-10 parts of a mixture of vitamin C and acerola cherry powder, 1-3 parts of a zinc lactate aqueous solution and 6-12 parts of nicotinic acid microcapsule liquid. By adding natural plant extracts and trace elements and supplementing by oral administration, the health-care beverage can endogenously improve physical quality, promote blood circulation, increase the oxidation resistance of the body of a drinker, and contribute to improving the body vitality and enhancing the immunity; the functional drinking water prepared by the invention is suitable for long-term storage, inhibits the growth of microorganisms, and maximally retains the biological activity of the microorganisms.

A general photobioreactor microbial growth rate prediction model and method thereof, the prediction model comprising a pretreatment module, a fluid dynamics calculation module, a light transmission module and a microbial growth rate mechanism module; first, a physical model of the photobioreactor is obtained; second, according to the obtained physical model and the calculation region, mesh division is carried out to construct the pretreatment module; then, the initial value of the growth parameter of the microbial particles in the photobioreactor is calculated; finally, the microbial growth rate in the photobioreactor is calculated and predicted through a coupled model of computational fluid dynamics, light intensity transmission law and microbial growth mechanism; the present application is based on physical modeling, and the computational fluid dynamics, light intensity transmission and microbial growth mechanism are coupled, thereby expanding the generality of the growth rate prediction; and the model can more accurately predict the microbial growth rate in any photobioreactor.

The utility model discloses a biological treatment device for acid mine wastewater. The biological treatment device comprises a connecting pipe, a peristaltic pump, an aeration device with an air compressor and an aeration pipe, a biological oxidation component, a biological reduction component, a water outlet tank and a water inlet tank, the biological oxidation part consists of an AMD tank, a peristaltic pump, a biological oxidation reactor, an iron precipitation reactor, a settling tank and a connecting pipe; the biological oxidation reactor is filled with biological filler, is provided with an aeration device, enters from the lower part and exits from the upper part and is beneficial to growth and attachment of microorganisms; the iron precipitation reactor is provided with a stirring device and can adjust the pH value of a solution; the settling tank is used for solid-liquid separation after an iron precipitation reaction and clarification of the solution; the biological reduction part consists of three peristaltic pumps, a biological reduction reactor 7 capable of realizing internal water circulation, heating, temperature measurement, sampling and bacterial liquid addition, two peristaltic pumps, two connecting pipes, three connecting pipes and a water outlet tank for solid-liquid separation and solution clarification; and the wastewater subjected to biological oxidation treatment is received by the water inlet tank and then flows to biological reduction for continuous treatment. The device has the advantages of purifying water and recycling Fe and Cu under the condition of low loss of Cu with low pH value, and being high in cost performance and the like.

The present invention relates to the use of an animal feed additive composition for controlling the growth of undesirable microorganisms, said composition comprising glycerides, vegetable oils, tannins and / or saponins, the glycerol ester is selected from the group consisting of glycerol monopropionate, glycerol dipropionate and glycerol tripropionate, glycerol monobutyrate, glycerol dibutyrate and glycerol tributyrate, glycerol monovalerate, glycerol divalerate and glycerol trivalerate, and mixtures thereof. The invention also relates to the use of the animal feed additive composition for promoting intestinal growth, alleviating or alleviating effects from necrotizing enteritis, modulating intestinal flora and / or as an additive to drinking water.

A microbiological testing device includes a growth medium support plate and a cap attached to the growth medium support plate, the growth medium support plate including a microbiological testing area, wherein the microbiological testing area includes at least one recess for receiving a microbiological growth medium, wherein the least one recess includes a bottom surface and at least one anchoring element protruding from the bottom surface, and wherein the at least one anchoring element includes an undercut for engaging with the microbiological growth medium. Further, a microbiological testing assembly and a cap for the microbiological testing device are described.

The invention relates to a workshop environment regulation and natural raw material synergistic preservative-free anticorrosion technology, relates to the technical field of food processing, and adopts the scheme that workshop environment regulation and natural raw material anticorrosion are combined to realize a synergistic effect. The method specifically comprises the steps that a multi-layer air filtering and disinfecting technology is adopted to enable a workshop to maintain 100 thousand-grade cleanliness, and the temperature and humidity are accurately controlled to be 22-24 DEG C and 40-60% RH through an automatic control system; meanwhile, natural components such as whey ferment powder and table vinegar powder are added into the product. The process can effectively control the growth of microorganisms, prolongs the shelf life of products such as bread to 90 days under the condition of not using any chemical preservative, and ensures that the safety, flavor and quality of the products are not influenced.

The invention relates to a potassium type neutralization regulation and control method in a sebacic acid fermentation process and application, a potassium hydroxide solution is used as a neutralizer, sebacic acid generated by microorganisms is neutralized in situ in the fermentation process, a product is accumulated in a high-solubility sylvite form, and the problem of sodion pollution caused by use of sodium hydroxide in a traditional process is avoided. The method comprises the core steps of strain activation, seed solution preparation, fermentation culture medium preparation, fermentation starting, pH dynamic regulation and control and product extraction. By automatically controlling the pH value to be 4.5-6.8, a 20-40% potassium hydroxide solution is fed, so that the fermentation acid production concentration reaches 180g / L or above. A potassium sulfate byproduct which is easy to dissolve in water is generated through subsequent acidification and crystallization, efficient separation can be achieved through washing, and a low-sodium / sodium-free high-purity sebacic acid product is obtained. According to the method, potassium ions are used as necessary elements for microbial growth to optimize a metabolic environment, so that the viscosity of fermentation liquor is remarkably reduced, dissolved oxygen transfer is improved, the environmental protection pressure of downstream extraction is relieved, and the production efficiency and the product quality are improved.

The invention belongs to the technical field of microbiological detection, and particularly relates to a high-throughput microbiological growth detector. Comprising a loading platform which is used for arranging a culture container; the mechanical shaking device comprises a mounting platform, a first driving motor, a transmission wheel, a transmission rod, a first transmission track and a second transmission track, the first driving motor is arranged at the upper end of the mounting platform, the transmission wheel is connected with an output shaft of the first driving motor, and the lower end of the transmission rod is eccentrically connected with the transmission wheel; the upper end of the transmission rod is rotationally connected with the loading platform, the first transmission track is vertically distributed, and the second transmission track is horizontally distributed. The first driving motor is used for driving the transmission wheel to rotate, so that the upper end of the transmission rod is driven to move relative to the first transmission track and the second transmission track, the loading platform can be driven to shake in the vertical direction and the horizontal direction, and a culture solution and microorganisms in the culture container can be uniformly mixed.

The embodiment of the invention discloses a sample analysis system and a control method thereof, and the sample analysis system comprises a blood culture module, a display module and a control module. The blood culture module comprises an incubation assembly and a detection assembly, the incubation assembly is used for loading at least one sample and incubating the sample, and the detection assembly is used for detecting microorganism growth parameters of the sample in the incubation assembly to obtain corresponding detection data; the control module is used for acquiring target detection data of the target sample, generating a growth curve of the target sample according to the target detection data, and displaying the growth curve of the target sample in the first display area through the display module; the growth curve of the target sample comprises a historical growth curve before the target sample is reloaded and an extended growth curve after the target sample is reloaded, and the historical growth curve and the extended growth curve are displayed in different styles. On the basis, the sample analysis system provided by the embodiment of the invention can improve the interaction experience of the user after the sample is reloaded.

The invention discloses a preparation method of a protonated carbon dot photocatalyst for coenzyme regeneration and application of the protonated carbon dot photocatalyst, manganese gluconate and L-aspartic acid are used for synthesizing carbon dots through a hydrothermal method, and then the protonated carbon dots are obtained through an acid leaching method. Photocatalysis coenzyme regeneration relates to transfer and utilization of two electrons and one proton, protonation treatment can affect the band gap structure of the catalyst and the electron and proton transfer problem in the photocatalysis process, and efficient regeneration of reduced nicotinamide adenine dinucleotide (NADH) coenzyme is achieved. The catalyst can be coupled with microorganisms, supports the growth of the microorganisms and converts CO2 into a high value-added product.

The application discloses a gel carrier suitable for low-temperature sewage treatment, a preparation method and application, and the gel carrier is divided into two layers, the upper layer of the gel carrier is a light-heat conversion layer, and the lower layer of the gel carrier is a microbial growth layer, anaerobic ammonia oxidation bacteria are embedded in the lower layer of the gel carrier, the thickness ratio of the light-heat conversion layer to the microbial growth layer is controlled to be 1-1.5:4, magnetic nanoparticles are added in the gel preparation process to form a heterojunction, the microenvironment temperature is improved through a light-heat-magnetic heat synergistic effect, the low-light limitation in winter is broken, and the reactor can be rapidly started under a low-temperature condition of 15 DEG C. The prepared gel carrier can decompose nitrogen-containing pollutants in wastewater in an anaerobic environment.

The application belongs to the technical field of food processing, and particularly relates to a fresh-keeping noodle and a preparation process thereof. The fresh-keeping noodle comprises the following preparation steps: (1) dough mixing and fermentation to obtain a primary fermented dough; (2) yeast activation, kneading of the primary fermented dough to a smooth surface, addition of activated yeast, secondary fermentation, and obtaining of a secondary fermented dough; (3) calendering, strip making, quantitative cutting, noodle boiling, and post-dipping metering packaging of the secondary fermented dough to obtain the fresh-keeping noodle. The dipping liquid comprises lactic acid, alcohol, and water. The alcohol plays a sterilization role, inhibits excessive water absorption of the noodles during the dipping process, and is beneficial to maintaining the toughness of the noodles. The lactic acid in the dipping liquid can adjust the pH and inhibit the growth of microorganisms in the noodles. The secondary fermentation consumes oxygen in the dough, forms a low-oxygen environment, inhibits the growth of spoilage bacteria, ensures the quality stability of the noodles during storage, and cooperatively improves the shelf life of the noodles.

The invention relates to a lactic acid bacteria composition and an application thereof in a low-temperature liquid biological feed, in particular to a lactic acid bacteria complex microbial inoculant suitable for a low-temperature environment (15-25 DEG C) and a fermentation process of the lactic acid bacteria complex microbial inoculant. The combination comprises pediococcus pentosaceus WP101 (the preservation number is CCTCC NO: M 20241055) and lactobacillus plantarum PC01 (the preservation number is CCTCC NO: M2022106), and the viable count of the combination is 25 million cfu / g feed according to the proportion of 1: 1. By optimizing the synergistic effect of strains and fermentation conditions, efficient fermentation in a low-temperature environment is realized, the content of lactic acid in the feed is remarkably increased, and growth of harmful microorganisms is inhibited. Animal experiments show that the fermented feed can reduce the diarrhea rate of piglets by 45.5% (Plt; 0.05), improve the daily gain of fattening pigs in the later period and the feed conversion rate, and reduce the loss of dry matters at the same time. The problem of energy consumption caused by the fact that traditional medium-temperature fermentation depends on heating and heat preservation is solved, technical support is provided for large-scale low-temperature liquid biological feed production, and economic and environmental benefits are achieved.