464 results about "Microbiological growth" patented technology

The invention discloses a system and method for identifying microbial contamination in food and cosmetics based on AI identification, and the method comprises the steps: collecting to-be-detected food or cosmetics as a detection sample, and obtaining the bacterial colony growth state of microorganisms in the detection sample; the method comprises the following steps: acquiring a microbial growth image through microscopic imaging equipment, and preprocessing the microbial growth image to obtain a target area of a microbial colony; constructing an AI recognition model by adopting a convolutional neural network, and obtaining the species and quantity of microorganisms in the detection sample; parameters of the AI recognition model are adjusted in real time according to the types and the number of the microorganisms, a preset microorganism limited standard threshold value is combined, a visual analysis report is generated, the AI recognition model is combined with a Faster R-CNN target detection method, precise classification and number statistics of the types of the microorganisms are achieved, a complete microorganism pollution analysis report is formed, and the method is suitable for popularization and application. And the accuracy and scientificity of the final identification result are improved.

An electrochemical sensor extracts information from biofluid systems by harnessing a nonlinear dynamic electrochemical model and stochastic voltage or current input. It uses a black-box approach that describes the fluid's state and predicts its evolution over time using a collection of model parameters, nonlinear dynamic measurement modes, and modeling techniques. For example, the sensor can use principal component analysis to reduce the set of (potentially hundreds) of model parameters to a handful of latent variables which evolve independently of each other. The sensor can use a set of these latent variables as a description of the state of the fluid. For a given sample fluid (e.g., milk containing contaminants), the sensor collects trajectories of the fluid state over time under varying conditions, permitting the training of a machine learning model to predict either fluid state trajectories or time until the fluid state crosses a given threshold (e.g., spoilage).

The embodiments disclose a method for testing organic plant materials, including detecting and associating machine-readable QR code identifiers at multiple testing locations within a facility, preparing samples at the multiple testing locations with microorganism-specific growth stimulator solutions to facilitate detection of contaminants, automatically detecting and analyzing environmental conditions at the multiple testing locations with a plurality of sensors, automatically correlating the captured images and the environmental conditions to the associated machine-readable QR code identifiers, analyzing and comparing the captured images and the environmental data against known harmful microorganisms, automatically generating contamination reports including detected infestations and remediation recommendations with mitigation actions and displaying the contamination reports and the recommendations and automatically generating and displaying a facility map on the graphical user interface with hotspot locations of the detected infestations of the contamination reports associated with the machine-readable QR code identifiers.

The invention relates to the technical field of water-soluble fertilizers, in particular to a water-soluble organic fertilizer and a preparation method and application thereof.The water-soluble organic fertilizer is prepared from, by mass, 40-100 parts of basic organic raw materials, 5-15 parts of microbial fermentation additives, 10-20 parts of nutrient element supplements and 3-7 parts of synergists; the microbial fermentation additive comprises a compound microbial agent and a microbial growth carrier, the nutrient element supplement comprises a nitrogenous fertilizer, a phosphatic fertilizer, a potash fertilizer and medium trace elements, and the synergist comprises fulvic acid and alginic acid. According to the water-soluble organic fertilizer and the preparation method and application thereof, efficient utilization and accurate supply of nutrients are achieved, the growth requirements of crops in plant factories are met, meanwhile, resource utilization of organic waste is promoted, and sustainable development of the plant factories is promoted.

The invention discloses a preparation method of a high-stability manual buffer solution, which comprises the following steps of: introducing two types of slow-release porous particle structures, namely a first type of particles loaded with acidic or alkaline components and a second type of particles loaded with silver nanoparticles, so that continuous slow release and dynamic adjustment of the components in the buffer solution are realized; the pH drift and the aging of a buffer system are effectively delayed; meanwhile, slow release of the silver nanoparticles endows the buffer solution with lasting antibacterial protection, microbial growth is remarkably inhibited, and due to the structural design, the long-term pH stability and storage safety of the buffer solution are remarkably improved, the clarity and physicochemical properties of the buffer solution are guaranteed, and the stability of the buffer solution is improved. The method greatly expands the application range in the fields of high-requirement biological experiments, medical detection, long-term sample storage and the like, the particle addition amount and the component proportion can be flexibly adjusted according to actual requirements, the method adapts to different pH ranges and application scenes, and various experiment requirements of protein purification, long-term sample storage, high-sensitivity analysis and detection are met.

The invention discloses a saline-alkali soil rhizosphere microdomain soil improvement and fertilization fertilizer and a preparation method thereof, and belongs to the technical field of saline-alkali soil treatment. The fertilizer is composed of a microbial inner core, a slow release fertilizer secondary outer layer and a soil conditioner outer layer, the microbial inner core is composed of a compound microbial agent (composed of nitrogen-fixing bacteria, phosphate solubilizing bacteria and live potassium bacteria), biochar and a polymer organic membrane material, the slow release fertilizer secondary outer layer is composed of a chemical fertilizer, an inhibitor (a nitrification inhibitor and / or a urease inhibitor) and amino acid, and the soil conditioner outer layer is composed of a soil conditioner outer layer. The soil conditioner outer layer is composed of a soil conditioner special for saline-alkali soil improvement. The method has the beneficial effects that (1) the pH value of the soil is adjusted and the soil structure is improved, so that the microbial flora can better adapt to the soil environment of the saline-alkali soil; and (2) the biochar and the isolating membrane effectively protect the activity of microorganisms, and the microorganisms are released when the pH value and the salt-based ions of the soil around the rhizosphere reach a lower level, so that the saline-alkaline obstacle of the growth of the microorganisms is overcome.

The invention discloses a soil microorganism carbon utilization efficiency determination method, and relates to the technical field of carbon utilization efficiency determination, and the method comprises soil sample pre-culture, multi-source 18O isotope gradient labeling, dynamic gas monitoring, microorganism respiration rate dynamic correction, and microorganism growth rate and carbon utilization efficiency acquisition. By labeling multi-source isotopes and quantifying contributions of different oxygen sources to microorganism DNA synthesis, underestimation of the carbon utilization efficiency caused by a traditional single labeling method is avoided, the problem of limitation existing in the current soil microorganism carbon utilization efficiency determination process is solved, the inhibition effect of too high local concentration on microorganism metabolism is reduced through gradient labeling, and the accuracy of soil microorganism carbon utilization efficiency determination is improved. The method has the advantages that marking uniformity is improved, multiple groups of soil samples are arranged, real-time change of microbial respiration is captured by means of dynamic monitoring, influence of isotope fractionation on carbon dioxide concentration calculation is eliminated, instantaneous errors of traditional static measurement are avoided, and accuracy of analysis results is improved to a certain extent.

The invention relates to the technical field of microbiological detection and image detection, and provides a microwell plate microbiological growth state detection method, system and equipment based on artificial intelligence. The system comprises an image acquisition module, an image segmentation module, a data set construction module, an image data enhancement module, an image classification module, a classification result determination module and a reasoning module, and is used for detecting whether bacteria grow in the microwell plate; or the system comprises an image regression module and a regression error evaluation module and is used for detecting the biomass of the thalli. According to the invention, a self-built photographing device can be adopted to realize on-site direct automatic detection without sampling, so that high-throughput automatic accurate analysis of the growth state of microorganisms in the microwell plate can be conveniently and quickly realized, experiment consumables and biological samples can be saved, the contamination risk can be reduced, and the experiment efficiency can be remarkably improved. Therefore, the working intensity of detection personnel is reduced, and the method has wide application value. Meanwhile, the complexity of detecting the growth state of the microorganisms in the pore plate is reduced, and popularization and application are facilitated.

The invention relates to preparation and technical methods of a composite saline soil modifier. The modifier is prepared from 30-40 parts of charcoal, 8-10 parts of oxalic acid, 15-20 parts of desulfurized gypsum, 3-6 parts of wood vinegar, 1-2 parts of microbial agent fermentation liquor and 30-40 parts of other auxiliary components. Wherein the microbial agent fermentation liquor is prepared by fermenting bacillus subtilis, the biochar is prepared by pyrolyzing rice husk at 600 DEG C for 2 hours, and the desulfurized gypsum is prepared by wet desulfurization; the preparation method of the modifier comprises the following steps: uniformly mixing the raw materials in proportion, drying until the water content is lower than 10%, crushing and packaging. During application, the fertilizer is uniformly applied to salinized soil according to the dosage of 100 kg / mu, the ploughing depth is 20-30 cm, and the soil is kept moist. Experimental results show that the modifier can significantly reduce the pH value of soil, increase the number of water-stable aggregates, increase the water retention capacity of soil, promote the growth of soil microorganisms and enhance the salt resistance of crops, and has a significant improvement effect on mild to moderate salinized soil.

This invention discloses a soil improvement and fertilization fertilizer for rhizosphere micro-domains in saline-alkali land and its preparation method, belonging to the field of saline-alkali land management technology. The fertilizer consists of a microbial core, a slow-release fertilizer sub-outer layer, and a soil conditioner outer layer. The microbial core is composed of a compound microbial agent (composed of nitrogen-fixing bacteria, phosphate-solubilizing bacteria, and potassium-active bacteria), biochar, and a high-molecular organic membrane material. The slow-release fertilizer sub-outer layer is composed of chemical fertilizers, inhibitors (nitrification inhibitors and / or urease inhibitors), and amino acids. The soil conditioner outer layer is composed of a soil conditioner specifically for saline-alkali land improvement. The advantages of this invention are: (1) it first adjusts the soil pH and improves the soil structure, so that the microbial community can better adapt to the soil environment of saline-alkali land; (2) the biochar and the isolation membrane effectively protect the microbial activity, and release the microorganisms when the soil pH and base ions around the rhizosphere reach a low level, overcoming the saline-alkali barrier to microbial growth.

An apparatus for mitigating contamination in a fuel supply is an ultrasonic generator coupled with a piezoelectric transducer that propagates ultrasonic waves through a fuel tank. In some embodiments the apparatus is used to mitigate the growth of microbial organisms. In other embodiments the apparatus is used to emulsify water into the fuel, particularly in diesel fuel. The apparatus addresses water and microbial growth found in boats, cars, and aircraft as well as in storage tanks such as those used at fuel stations. In some embodiments, the fluid is diesel fuel.

Described herein are methods and compositions for using microbial agents (probiotics) and agents that promote growth of certain microbes (prebiotics) for management (including prevention and treatment) of musculoskeletal disorders, including osteoporosis, osteopenia, Paget's disease, stunting, osteoarthritis, osteomyelitis, and delayed or non-union fractures. Also described herein are methods and compositions for using probiotics and prebiotics for management of inflammation, and symptoms of menopause.

The invention discloses a method for synergistically regulating and controlling microbial growth in stages based on light / carbon / nutrient substances, which comprises the following steps: according to the growth cycle of photosynthetic microorganisms, the photosynthetic microorganisms are cultured by changing control parameters, and the control parameters are one or more of dynamic regulation illumination intensity, nutrient element or carbon source supply. According to the method disclosed by the invention, through dynamic illumination and staged nutrient element and carbon source supply, the light energy and nutrition utilization efficiency in the photosynthetic microorganism culture process is remarkably improved. Compared with an existing constant illumination and traditional nutrition supply mode, the light intensity, the light period and the nutrition supply strategy can be dynamically adjusted according to the actual requirements of microorganisms in different growth stages, light inhibition and nutrient waste are avoided, rapid growth of the microorganisms and accumulation of target products are effectively promoted, and therefore the culture yield and efficiency are remarkably improved. The method provided by the invention can be widely applied to the fields of environmental governance, biofuel, medicine, food and the like.

The invention discloses an activated sludge self-adaptive regulation and control method based on multi-source data mining, and belongs to the technical field of sludge treatment.The activated sludge self-adaptive regulation and control method comprises the steps that a microflora feature data set is obtained from a sewage treatment system; according to the microflora feature data set, analyzing the sludge state by adopting a classification model, and judging the growth stage of microorganisms; determining the current sludge discharge load level; if the current sludge discharge load level exceeds a preset threshold value, deducing future sludge state evolution by adopting a prediction model according to the type of the current microbial growth stage and the microbial community characteristic data set to obtain an aeration intensity adjustment threshold value; determining sludge discharge configuration parameters by matching the aeration intensity adjustment threshold value with the current aeration intensity; and taking the aeration intensity adjustment threshold value and the sludge discharge configuration parameter as a sewage treatment parameter configuration scheme. The activated sludge self-adaptive regulation and control method based on multi-source data mining solves the problem that regulation and control of activated sludge are difficult to realize accurately at present.

The invention relates to the technical field of microbial fermentation, in particular to a microbial fermentation control system based on an intelligent sensor, and the system comprises a data collection module which is used for periodically collecting environment information and reactant information in a fermentation tank; the data analysis module is used for constructing a fermentation coupling model based on the information change condition of reactants in the fermentation tank in the target time period, constructing a microorganism growth curve according to the environment information change condition, and determining a microorganism growth key area based on the microorganism growth curve; the data judgment module is used for determining a microbial fermentation process based on the fermentation coupling model and the microbial growth key area, and judging whether microbial fermentation meets a preset standard or not according to the microbial fermentation process; and the control and adjustment module is used for determining a control and adjustment mode of the fermentation tank based on a judgment result that the microbial fermentation process does not meet the preset standard. The microbial fermentation environment can be accurately controlled, so that the fermentation effect and the fermentation efficiency are improved.

The present invention relates to the in vitro use of a cationic hydroxyalkyl guar for maintaining or increasing the growth rate of microorganisms, wherein said cationic hydroxyalkyl guar has an average molecular weight of between 2,000 Daltons and 90,000 Daltons.

The invention relates to the technical field of food processing, in particular to a chicken quality preservation treatment method based on sectional regulation, which is characterized in that chicken is pretreated by a composite biological preservative solution, and ice storage treatment and staged storage are combined, so that microbial growth and enzymatic activity of the chicken are effectively inhibited; fat oxidation and protein degradation are delayed, so that the chicken still keeps excellent quality in long-term storage; in the staged storage process, key indexes are monitored in real time or periodically, a threshold value comparison and stage switching mechanism is established, and accurate dynamic regulation and control of the staged modified atmosphere packaging environment under the condition of dynamic deterioration of the chicken quality are achieved. Through segmented regulation and control, juice loss, color deterioration and decay of the chicken in the storage process can be effectively prevented, so that the sensory quality and the nutritional quality of the chicken are maintained, and meanwhile, the gas utilization efficiency in the storage environment is also remarkably improved.

The invention discloses an aquatic product cold chain transportation monitoring system which is characterized in that the system comprises a hardware layer and a software layer, the hardware layer comprises a sensing control module, a structure bearing module and a communication interaction module, and the software layer comprises a data management module, an intelligent calculation module and a business application module; the sensing control module comprises an environment sensing sub-module and an equipment control sub-module, the structure bearing module comprises a compartment main body sub-module and a cargo protection sub-module, and the communication interaction module comprises an edge calculation sub-module and a vehicle-mounted interaction sub-module. According to the application, a real-time monitoring-dynamic prediction-intelligent regulation and control microbial inhibition system is constructed, a high-precision sensor is used for collecting environmental data in real time, and an LSTM-Attention model is combined for accurately pre-judging the growth trend of microorganisms, so that the effects of early warning before the colony count reaches a dangerous threshold value and automatically coordinating equipment to start targeted bacteriostatic measures are achieved, and the safety of the system is improved. And the loss rate of aquatic products caused by microbial pollution is reduced.

This invention discloses a multi-scale prediction method and system for the maximum growth rate of soil microorganisms based on genomic features, involving the interdisciplinary fields of microbial ecology and genomics. The method includes: genomic data preparation, core genomic feature extraction, single-scale growth rate prediction, community-scale extrapolation, and result verification and output. The system includes a genomic data processing unit, a core feature extraction module, a growth rate prediction model, a community-scale extrapolation module, and a result visualization and verification unit. This invention overcomes the limitations of traditional culture methods and in-situ measurement methods, achieving high-throughput and accurate prediction of the growth rates of culturable and uncultured microorganisms, and solving the problems of low coverage, fragmented scales, and high costs of existing technologies. It is applicable to soil microbial function assessment, carbon cycle model parameterization, and ecosystem management.

The invention provides a phosphorus release improving type compound fertilizer based on a fungal extracellular polymer, a preparation method and application, and belongs to the technical field of microorganisms, the phosphorus release improving type compound fertilizer is prepared by compounding the extracellular polymer generated by specific rhodotorula mucilaginosa WTSX01 and inorganic phosphate according to the mass ratio of 1: 9; the preparation method comprises the following steps: extracting and purifying high-activity EPS from a strain fermentation broth by a low-temperature dialysis method, separating lipid from EPS as a natural adhesive by a cosolvent extraction method, and finally mixing EPS, lipid and inorganic phosphate. The invention also discloses a synergistic application of the fertilizer and a phosphorus-solubilizing fungus aspergillus niger (CCTCC No. M2023240), so that the release efficiency of phosphorus in soil is improved; the fertilizer provided by the invention provides exclusive nutrition for phosphate-solubilizing microorganisms, effectively overcomes indigenous microorganism competition, remarkably promotes microorganism growth and phosphorus release, and realizes reduction and synergism of phosphate fertilizer and yield increase of crops.

The application discloses a kind of light-assisted anticorrosion coating materials and preparation method based on metal coordination structure, the light-assisted anticorrosion coating material is on the basis of traditional anticorrosion metal zinc layer continue to be coordinated with the metal coordination polymer Co (bpy) (dhbq) (bq) with photoelectric conversion capability, and form coordination complex Co (bpy) (dhbq) (bq) @Zn.Because metal coordination polymer Co (bpy) (dhbq) (bq) itself can additionally generate photo-generated electron and photo-generated current after being illuminated, provide redundant charge for Zn layer, make it be in lower potential, strengthen the function of cathodic protection.Corrosion can be inhibited through this mechanism, prolong the service life of Zn layer, provide efficient, green anticorrosion protection for the steel material that needs to be protected.In addition, metal coordination light-assisted anticorrosion technology can also inhibit marine bioattachment by slowly releasing metal ions Co, prevent biofilm formation, reduce microbial growth, prolong the service life of protected material in marine environment, also applicable to underwater bioinduced corrosion and the protection of fouling metal structure.

The utility model discloses a gas circulation type meat product fermentation device, which belongs to the technical field of flavor meat product processing, and comprises a fermentation part, a gas circulation part and a gas circulation part, the air supply part is communicated with the side surface of the fermentation part and is used for introducing air into the fermentation part; the flow guide part is communicated with one end of the air supply part so as to uniformly introduce air at a preset temperature into the fermentation part; the air return part is arranged between the fermentation part and the flow guide part in a communicating manner and realizes air flowing through the driving part; a three-way pipe valve is also arranged on the air return part, and the three-way pipe valve is communicated with an external pipeline so as to introduce fresh air or circulating air into the fermentation part. According to the fermentation device, traditional mechanical stirring is replaced with gas flow, mass transfer and heat transfer are achieved through gas circulation, growth and metabolism of microorganisms are promoted, and therefore the characteristics of meat products are converted, the flavor is improved, and the shelf life is prolonged.

A method for controlling microbial growth in a sugar processing system is disclosed, wherein the method comprises adding a peroxy acid into water of a flume system used for transporting a sugar-containing plant material from a delivery or storage location to a wash system. In one non-limiting example embodiment, the sugar-containing plant material comprises sugar beets, and the peroxy acid comprises peracetic acid.

Silk performance apparel and methods of preparing the same are disclosed herein. In some embodiments, silk performance apparel includes textiles, fabrics, consumer products, and other materials that are coated with aqueous solutions of pure silk fibroin-based protein fragments. In some embodiments, coated apparel products exhibit surprisingly improved moisture management properties and increased resistance to microbial growth.

The invention discloses a photo-assisted anti-corrosion coating material based on a metal coordination structure and a preparation method, the photo-assisted anti-corrosion coating material is continuously coordinated with a metal coordination polymer Co (bpy) (dhbq) (bq) with photoelectric conversion capability on the basis of a traditional anti-corrosion metal zinc layer to form a coordination compound Co (bpy) (dhbq) (bq) (at) Zn. Due to the fact that the metal coordination polymer Co (bpy) (dhbq) (bq) can additionally generate photo-generated electrons and photo-generated currents after being illuminated, redundant charges are provided for the Zn layer, the Zn layer is made to be in a low potential, and the cathode protection function is enhanced. Through the mechanism, corrosion can be inhibited, the service life of the Zn layer can be prolonged, and efficient and green anti-corrosion protection is provided for steel and iron materials needing to be protected. In addition, by slowly releasing metal ions Co, the metal coordination light-assisted anticorrosion technology can inhibit marine organism adhesion, prevent biofilm formation, reduce microbial growth and prolong the service life of a protected material in a marine environment, and is also suitable for underwater organism induced corrosion and protection of stained metal structures.

The invention discloses an intelligent distribution scheduling method and device for egg products and a medium, and relates to the technical field of intelligent scheduling, and the method comprises the steps: collecting biological characteristic parameters of target egg products in real time, carrying out the feature encryption processing through an edge calculation node to form private data packets, receiving the private data packets of all suppliers through a message queue service, and sending the private data packets to a server; calling a homomorphic encryption library to execute weighted average aggregation on the encrypted feature vectors; and loading real-time road condition data, compartment temperature and humidity distribution and a vibration spectrum in a digital twin sandbox based on a global egg rheology knowledge graph and a microbial growth kinetic model, and coupling to solve a product risk value of a protein shear stress accumulation equation and a flora growth equation. By constructing a global egg product rheology knowledge graph and a microbial growth kinetic model, the time-space coupling characteristics of biomechanical characteristics and microbial activity of egg products in transportation are accurately captured, and the quality risk quantification precision and the transportation strategy reliability are improved.

A composition (100) for inhibiting growth of microbial contamination is disclosed The composition includes a plurality of volatile antimicrobial components (110) including at least one of potassium sorbate and sorbic acid. The composition further includes a plurality of non-volatile antimicrobial components (120) including natamycin. A weight ratio of the plurality of volatile antimicrobial components to the plurality of non-volatile antimicrobial components is in the range of 10:2 to 10:1. The composition is placed at a distance from a target surface to inhibit microbial growth on the target surface by releasing the plurality of volatile antimicrobial components and the plurality of non-volatile antimicrobial components into a surrounding air. The target surface is exposed to microbial contaminants. moisture and humidity.

The invention relates to a microbial growth activation accelerant based on rare earth elements as well as a preparation method and application thereof, and belongs to the field of resources and environments. The microbial growth activation accelerant based on the rare earth elements is prepared from the following components: 1 to 150g of a rare earth compound, 0.2 to 2g of pantothenic acid, 0.2 to 2g of nicotinic acid, 0.02 to 0.1 g of nicotinamide and 1000g of deionized water, the rare earth compound is one or combination of more of chlorides of lanthanum, cerium, praseodymium, neodymium, promethium, samarium, europium, gadolinium, terbium, dysprosium, holmium, erbium, thulium, ytterbium, lutetium, scandium and yttrium, nitric acid compounds and sulfuric acid compounds. A large number of cheap light rare earth elements are used as raw materials of the microbial accelerator, a new utilization approach is provided for the light rare earth elements, the light rare earth elements are converted into high-added-value products, pollution of the light rare earth elements to the environment is reduced, and meanwhile new elements are introduced into the field of microbial accelerators. More importantly, the preparation method of the microbial growth activation accelerant based on the rare earth elements provided by the invention is simple in production process and convenient for large-scale production.

Described herein are Staphylococcal toxins inspired peptides (STIPs) with antimicrobial activity, compositions and kits comprising the peptides, and use of the peptides to treat microbial infections. Also described are methods of inhibiting or preventing the growth of a microorganism, and methods of treating microbial infections such as bacterial infections including MRSA, and fungal infections including C. albicans.

To provide a method for detecting the growth of microorganisms that can accurately detect a droplet in which microorganisms have grown, to provide a method for obtaining microorganisms, to provide a kit for detecting the growth of microorganisms, to provide a kit for obtaining microorganisms, and to provide use of dye as microbial growth reporter.SOLUTION: A method for detecting the growth of microorganisms comprises: producing a droplet containing a dye whose fluorescence intensity changes by interacting with a microorganism and a membrane component possessed by the microorganism, in W / O emulsion; culturing the microorganism within the droplet; and detecting the growth of the microorganism based on the fluorescence intensity of the dye.SELECTED DRAWING: Figure 1