896 results about "Microbiological growth" patented technology

The invention relates to the technical field of water quality monitoring, in particular to a water quality monitoring analysis method and system of a water conservancy system. The method comprises the following steps: acquiring area scale and water area type distribution, and carrying out sensing planning layout and water quality index real-time monitoring to obtain a water conservancy system water quality index data set; performing regional water quality index distribution coupling and regional time step division on the water conservancy system to obtain a corresponding water conservancy pH value, water conservancy dissolved oxygen concentration, water conservancy turbidity, water conservancy heavy metal concentration and water conservancy microorganism concentration; performing microorganism growth efficiency evaluation and microorganism pollution attenuation analysis on the water conservancy microorganism concentration to obtain a water conservancy area microorganism pollution degradation attenuation rate; and performing water quality pollution anomaly quantification and anomaly category decision management on the water quality index data set of the water conservancy system to generate a water quality anomaly category management strategy so as to execute corresponding regional water quality category management work of the water conservancy system. The accuracy and efficiency of water quality monitoring can be improved.

The invention discloses a system and method for identifying microbial contamination in food and cosmetics based on AI identification, and the method comprises the steps: collecting to-be-detected food or cosmetics as a detection sample, and obtaining the bacterial colony growth state of microorganisms in the detection sample; the method comprises the following steps: acquiring a microbial growth image through microscopic imaging equipment, and preprocessing the microbial growth image to obtain a target area of a microbial colony; constructing an AI recognition model by adopting a convolutional neural network, and obtaining the species and quantity of microorganisms in the detection sample; parameters of the AI recognition model are adjusted in real time according to the types and the number of the microorganisms, a preset microorganism limited standard threshold value is combined, a visual analysis report is generated, the AI recognition model is combined with a Faster R-CNN target detection method, precise classification and number statistics of the types of the microorganisms are achieved, a complete microorganism pollution analysis report is formed, and the method is suitable for popularization and application. And the accuracy and scientificity of the final identification result are improved.

The invention discloses recombinant escherichia coli for producing D-psicose and a preparation method of the recombinant escherichia coli, and belongs to the field of genetic engineering. The technical problem to be solved in the invention is how to supply microorganisms for growth by using glycerol, acetic acid, fatty acid and the like as raw materials and prepare D-psicose by using glucose as a raw material. The invention provides recombinant Escherichia coli, which uses glycerol, acetic acid, fatty acid and the like as carbon sources to amplify (grow) the recombinant Escherichia coli, and uses D-glucose as a substrate to prepare D-psicose. According to the recombinant escherichia coli provided by the invention, glycerol, acetic acid, fatty acid and the like can be used as carbon sources for microbial growth, and D-psicose is synthesized by taking D-glucose as a raw material after the microbial growth reaches a certain biomass. Furthermore, a corresponding technical route for synthesizing the D-psicose is established.

The invention discloses a water quality monitoring system applied to sewage treatment, and relates to the technical field of sewage quality monitoring. Before microorganisms are added, the uniformity of water quality is monitored and analyzed, the sewage treatment mode is confirmed, and during layered treatment, the water quality is monitored and analyzed. The method comprises the following steps: analyzing a microorganism combination scheme and a microorganism addition scheme during sewage treatment of each deep layer, during overall treatment, carrying out water quality monitoring on the microorganism combination scheme and the overall microorganism addition scheme of the sewage after microorganisms are added, and then carrying out corresponding adjustment, so that the uniformity and uniformity of the water quality treatment progress are ensured; the method is beneficial to maintaining the stability of a microorganism growth environment, so that microorganisms continuously and efficiently work in a relatively stable environment, the decomposition speed of pollutants in sewage is increased, the stability of effluent quality is ensured, the difficulty of subsequent microorganism control and regulation is reduced, the overall sewage treatment effect is improved, microorganism waste is not easily caused, and the method is suitable for industrial production. The operation cost is reduced.

The present application discloses a recombinant bacterium for producing D-psicose by fermentation and a preparation method thereof, and belongs to the field of food chemical industry. The technical problem to be solved by the present application is: how to obtain a recombinant bacterium that uses methanol as a raw material for bacterial growth and glucose as a raw material to synthesize D-psicose. By subjecting the recipient bacteria to a purposeful transformation, the microbial strain is provided with the ability to accumulate microbial biomass using methanol and synthesize D-psicose using D-glucose, while preventing the use of D-glucose for microbial growth so that D-glucose is completely converted into D-psicose, thereby improving substrate utilization efficiency. The recombinant polymorphic Hansen yeast provided in the present application can realize the use of methanol and other compounds as raw materials for microbial growth, and after the microbial growth reaches a certain biomass, D-psicose is synthesized using D-glucose as a raw material. And then establish a corresponding technical route for synthesizing D-psicose.

The invention relates to the field of microbial culture management, in particular to a microbial culture management system and method. The method comprises the following steps: calculating growth factors based on the number of microorganisms in a microorganism growth process and microorganism culture environment parameters which are collected in real time, and calculating a predicted value of the number of microorganisms at a future moment; detecting the growth state of the microorganisms based on the predicted value of the number of the microorganisms in the future; the initial adjustment amount of the microbial culture environment parameters is obtained through a multi-parameter nonlinear optimization algorithm, the final adjustment amount of the microbial culture environment parameters is obtained by introducing a synergistic effect mechanism, and the microbial culture environment parameters are adjusted. The problems that traditional microbial culture environment parameters depend on manual monitoring and experience adjustment, so that response is lagged, and adjustment is not accurate enough are solved; the future growth trend of microorganisms is difficult to accurately predict, so that the culture management reaction is delayed; the problems that the microorganism culture environment is imbalanced and optimal management cannot be realized due to the fact that microorganism culture environment parameters cannot be automatically optimized are solved.

The invention belongs to the technical field of microorganisms, and particularly relates to a microorganism detection culture device and method.The system comprises an intelligent environment control system, and a sensor monitors the temperature, humidity and gas concentration of a culture environment in real time to ensure that environment parameters needed by microorganism growth are kept in the optimal state; the automatic sample processing and operating system avoids pollution or errors caused by manual intervention through a mechanical arm or an automatic transmission device; the real-time image monitoring and analyzing system is used for monitoring the growth state of the microorganisms in the culture dish; the camera can continuously shoot images in the culture process; the rapid detection module rapidly obtains activity information of microorganisms, rapid detection is achieved, and time needed by traditional culture is shortened. According to the invention, accurate control, automatic operation and rapid detection are realized, and an efficient, safe and intelligent solution is provided for microbial culture and detection.

Methods of forming spinel ferrite nanoparticles containing a chromium-substituted copper ferrite as well as properties (e.g. particle size, crystallite size, pore size, surface area) of these spinel ferrite nanoparticles are described. Methods of preventing or reducing microbe growth on a surface by applying these spinel ferrite nanoparticles onto the surface in the form of a suspension or an antimicrobial product are also described.

The invention provides lactobacillus mucilaginosus and application thereof, and belongs to the technical field of microorganisms. According to the present invention, the Latin of the Lactobacillus mucus fermentation is Limosilactobacillusfermentum, the preservation name is Lactobacillus mucus fermentation MQ10, the preservation place is China Center for Type Culture Collection (CCTCC), the preservation date is July 22, 2024, and the preservation number is CCTCC NO: M 20241616. The strain has a broad-spectrum antibacterial effect and can inhibit growth and reproduction of various microorganisms. The biological fresh-keeping product prepared from the fresh-keeping agent can reduce the decay rate of food and the browning rate of fruit stems of fruits and vegetables, maintain the content and hardness of soluble solids, slow down the aging decay speed of the food in the storage period and prolong the storage period. Due to the characteristics, the lactobacillus mucilaginosus is expected to become a green and environment-friendly biological fresh-keeping agent, and the quality and the safety of foods are improved.

The invention relates to the technical field of bioengineering fermentation, and discloses a bioengineering fermentation method, through multiple groups of sensors arranged in a fermentation reactor, key data parameters in the fermentation reactor can be detected and monitored in real time, an operator can be helped to quickly and accurately know the fermentation state of a material in the fermentation reactor, and the fermentation efficiency of the material in the fermentation reactor is improved. Real-time and reliable working parameter adjustment and control are achieved, a multi-parameter dynamic optimization type biological engineering fermentation method is adopted, key parameters in fermentation engineering are accurately analyzed and predicted through dynamic parameter collection in cooperation with a mathematical model and an algorithm, prediction is accurate and reliable, reliable data support can be provided for dynamic regulation and control, and the method is suitable for large-scale popularization and application. The fermentation conditions are dynamically adjusted, so that the growth and metabolism of microorganisms are optimized, the yield and quality of products are improved, the fermentation period is shortened, the production cost is reduced, reliable experience and data support can be provided for subsequent fermentation work in cooperation with data recording, and the fermentation efficiency and the product quality are further optimized.

The present invention discloses a biofilm multi-stage treatment device for industrial wastewater with a telescopic mechanism, including a multi-stage treatment device body, a biological reaction tank and an aeration rod, the bottom of the aeration rod is fixedly connected to an aeration pipe, the outer wall of the aeration pipe is fixedly connected to an air dispersion nozzle, and the treatment device also includes: a receiving block and a disc. In the present invention, the first screw is driven to rotate by rotating the handle, and the two groups of first screw sleeves on the first screw move synchronously toward the center or both sides, the first connecting rod and the second connecting rod are fixedly connected to the first screw sleeve through a fixed rod, and the center of the first connecting rod and the second connecting rod is connected through a rotating shaft, so that the first connecting rod and the second connecting rod are synchronously expanded outward or contracted inward, and the disc at the bottom can synchronously adjust the spacing accordingly, thereby improving the growth environment of microorganisms, improving the purification efficiency of microorganisms, and improving the use effect of the product.

An electrochemical sensor extracts information from biofluid systems by harnessing a nonlinear dynamic electrochemical model and stochastic voltage or current input. It uses a black-box approach that describes the fluid's state and predicts its evolution over time using a collection of model parameters, nonlinear dynamic measurement modes, and modeling techniques. For example, the sensor can use principal component analysis to reduce the set of (potentially hundreds) of model parameters to a handful of latent variables which evolve independently of each other. The sensor can use a set of these latent variables as a description of the state of the fluid. For a given sample fluid (e.g., milk containing contaminants), the sensor collects trajectories of the fluid state over time under varying conditions, permitting the training of a machine learning model to predict either fluid state trajectories or time until the fluid state crosses a given threshold (e.g., spoilage).

According to the microbial flora culture method for efficiently degrading the organic pollutants, three continuous culture stages of enrichment, domestication and optimization are adopted, parameters such as the temperature, the pH value, the rotating speed and the ventilatory capacity are accurately controlled in each stage, and growth of microorganisms with degradation capacity is promoted. Through multi-stage culture and domestication, the obtained flora has efficient degradation capability on organic pollutants such as petroleum hydrocarbons, pesticides and polycyclic aromatic hydrocarbons, and has strong adaptability to pollutants with different concentrations. The 16S rRNA gene is used for sequencing and identifying the flora, and composition and dominant strains are clear. The flora is prepared into a microbial inoculum, and the microbial inoculum is preserved by freeze drying or liquid nitrogen, so that the activity can be effectively maintained. The flora can be widely applied to the fields of polluted soil and water body remediation, industrial wastewater treatment and the like, is green and environment-friendly, is relatively low in cost, avoids secondary pollution, conforms to the concept of sustainable development, and has relatively high economic and environmental values.

The invention belongs to the technical field of food fermentation, and provides a lactobacillus plantarum CMRC 1L and application thereof. The lactobacillus plantarum CMRC 1L disclosed by the invention is separated from a sour meat sample in Maojia village region in Lijiang City, Yunnan Province, grows well on an MRS culture medium, is high in acid production speed, has the biological characteristics of salt resistance, frostbite resistance, inhibition of growth and reproduction of spoilage microorganisms and the like, has a good fermentation effect on various plant proteins, and is an excellent strain for preparing a leavening agent for production. The invention also provides a leavening agent containing the strain and application of the leavening agent in vegetable protein meat products. Compared with the vegetable protein jerky without the leavening agent, the vegetable protein jerky product prepared by the preparation method provided by the invention has the advantages of stable quality, fresh and sweet fermentation fragrance, no beany flavor and good flavor and taste. The strain CMRC 1L has great potential when being applied to production of vegetable protein meat products.

The embodiments disclose a method for testing organic plant materials, including detecting and associating machine-readable QR code identifiers at multiple testing locations within a facility, preparing samples at the multiple testing locations with microorganism-specific growth stimulator solutions to facilitate detection of contaminants, automatically detecting and analyzing environmental conditions at the multiple testing locations with a plurality of sensors, automatically correlating the captured images and the environmental conditions to the associated machine-readable QR code identifiers, analyzing and comparing the captured images and the environmental data against known harmful microorganisms, automatically generating contamination reports including detected infestations and remediation recommendations with mitigation actions and displaying the contamination reports and the recommendations and automatically generating and displaying a facility map on the graphical user interface with hotspot locations of the detected infestations of the contamination reports associated with the machine-readable QR code identifiers.

The invention discloses a treatment method for improving the fresh-keeping effect of fresh-cut eggplants, which comprises the following steps: cleaning, pre-cooling and cutting fresh eggplants to obtain fresh-cut eggplants; soaking the fresh-cut eggplants in an anti-browning soaking solution for treatment, and draining off water after the treatment is completed; spraying a film coating liquid on the surface of the drained eggplant; a calcium chloride solution is sprayed on the surfaces of the sprayed eggplants, and the eggplants are air-dried at room temperature until the surfaces do not have obvious moisture; and packaging and storing the treated eggplants. According to the comprehensive fresh-keeping method for the fresh-cut eggplants based on the double-layer coating technology, the first layer is an anti-browning soaking solution, and the problems of browning and texture softening are solved; and the second layer is functional coating spraying liquid and is used for controlling gas exchange and microorganism growth. According to the method, multiple fresh-keeping barrier technologies are effectively integrated, green and natural fresh-keeping components are completely adopted, the shelf life of the fresh-cut eggplants is remarkably prolonged through scientific formula design and a modern food processing technology, and meanwhile good sensory quality and nutritional value are kept.

The invention relates to the technical field of sewage treatment, in particular to a modified basalt fiber, a preparation method of the modified basalt fiber and a biological membrane filler containing the modified basalt fiber. The prepared basalt fibers and polyester fibers are blended into fiber bundles which are blended on the fixed base layer to form the biological membrane filler, the fiber bundles are distributed on the surface of the fixed base layer in a seaweed shape, the fiber bundles are distributed in a vertically staggered mode, more attachment points can be provided for growth of microorganisms, meanwhile, the contact time of water flow and a biological membrane is prolonged, and the biological membrane filler is more stable. The biofilm culturing amount and the biofilm culturing speed of the biofilm are improved, and the biodegradability of the biofilm is enhanced; meanwhile, the fixed base layer is made of a flexible material, so that compression during transportation is facilitated, and the storage and transportation cost is reduced.

The invention provides a method for regulating and controlling the expression intensity of a prpB gene promoter, specifically, betaine is added into an expression system containing the prpB gene promoter, and the expression intensity of the prpB gene promoter can be regulated and controlled to be improved in the logarithmic phase and / or reduced in the stable phase of microbial growth. By utilizing the method, the expression level of a target gene in an expression system can be accurately regulated and controlled.

The present invention primarily relates to a mixture comprising or consisting of one or more compound(s) of formula (I) or (II) as defined herein and one or more further antimicrobial agent(s) and it relates to a product comprising a mixture as defined herein. Moreover, the present invention relates to the use of one or more compound(s) of formula (I) or (II) as defined herein to improve the antimicrobial effect of one or more further antimicrobial agent(s) and to the use of one or more further antimicrobial agent(s) to improve the antimicrobial effect of one or more compound(s) of formula (I) or (II) as defined herein. The present invention further relates to the use of a mixture as defined herein as an antimicrobial agent and to a process as defined herein for improving the antimicrobial effect of one or more compound(s) as defined in step (a) and / or of one or more compound(s) as defined in step (b) of the process. Moreover, the present invention relates to a process for inhibiting or preventing the growth of one or more microorganisms in a product or semi-finished product and to a process for inhibiting or preventing the growth of one or more microorganisms on a mammal's skin or mucosa and / or for inhibiting or preventing the growth of one or more microorganisms on / in a surface. Moreover, the present invention relates to a mixture or compound as defined herein for use as an antimicrobial agent for inhibiting or preventing the growth of one or more microorganisms on a mammal's skin or mucosa. Finally, the present invention relates to the use of one or more compound(s) as defined herein as antimicrobial agent(s) and to a process as defined herein for inhibiting or preventing the growth of one or more microorganisms on a mammal's skin or mucosa, and / or for inhibiting or preventing the growth of one or more microorganisms in a product or semi-finished product, and / or for inhibiting or preventing the growth of one or more microorganisms on / in a surface.

The invention relates to the technical field of comprehensive utilization of solid wastes, and discloses a soil conditioner prepared from coal-based solid wastes and a preparation method thereof, the soil conditioner comprises the following raw materials by weight: 80-100 parts of coal-based solid wastes, 5-15 parts of an inorganic salt material, 100-150 parts of pure water, 1-10 parts of a microbial agent fertilizer and 20-50 parts of crop straw biochar. According to the scheme, the inorganic salt is added, so that multivalent cations such as calcium, iron and aluminum are loaded on the coal-based solid waste and can be replaced with exchangeable sodium ions on soil colloid, and the soil desalting efficiency is improved; the crop straw biochar can increase the content of nutrient elements such as carbon, nitrogen and phosphorus for soil; the porous structure of the modified coal-based solid waste can provide a proper place for growth and reproduction of microorganisms, and the soil microenvironment is improved; the soil conditioner is simple in raw material composition, the coal-based solid waste is used as the main raw material for improving the saline-alkali soil, the source is wide, the production cost is low, pollution to the environment is avoided, and large-area popularization and application in saline-alkali soil areas can be facilitated.

The invention relates to the technical field of water-soluble fertilizers, in particular to a water-soluble organic fertilizer and a preparation method and application thereof.The water-soluble organic fertilizer is prepared from, by mass, 40-100 parts of basic organic raw materials, 5-15 parts of microbial fermentation additives, 10-20 parts of nutrient element supplements and 3-7 parts of synergists; the microbial fermentation additive comprises a compound microbial agent and a microbial growth carrier, the nutrient element supplement comprises a nitrogenous fertilizer, a phosphatic fertilizer, a potash fertilizer and medium trace elements, and the synergist comprises fulvic acid and alginic acid. According to the water-soluble organic fertilizer and the preparation method and application thereof, efficient utilization and accurate supply of nutrients are achieved, the growth requirements of crops in plant factories are met, meanwhile, resource utilization of organic waste is promoted, and sustainable development of the plant factories is promoted.

The invention discloses a preparation method of a high-stability manual buffer solution, which comprises the following steps of: introducing two types of slow-release porous particle structures, namely a first type of particles loaded with acidic or alkaline components and a second type of particles loaded with silver nanoparticles, so that continuous slow release and dynamic adjustment of the components in the buffer solution are realized; the pH drift and the aging of a buffer system are effectively delayed; meanwhile, slow release of the silver nanoparticles endows the buffer solution with lasting antibacterial protection, microbial growth is remarkably inhibited, and due to the structural design, the long-term pH stability and storage safety of the buffer solution are remarkably improved, the clarity and physicochemical properties of the buffer solution are guaranteed, and the stability of the buffer solution is improved. The method greatly expands the application range in the fields of high-requirement biological experiments, medical detection, long-term sample storage and the like, the particle addition amount and the component proportion can be flexibly adjusted according to actual requirements, the method adapts to different pH ranges and application scenes, and various experiment requirements of protein purification, long-term sample storage, high-sensitivity analysis and detection are met.

The invention discloses a saline-alkali soil rhizosphere microdomain soil improvement and fertilization fertilizer and a preparation method thereof, and belongs to the technical field of saline-alkali soil treatment. The fertilizer is composed of a microbial inner core, a slow release fertilizer secondary outer layer and a soil conditioner outer layer, the microbial inner core is composed of a compound microbial agent (composed of nitrogen-fixing bacteria, phosphate solubilizing bacteria and live potassium bacteria), biochar and a polymer organic membrane material, the slow release fertilizer secondary outer layer is composed of a chemical fertilizer, an inhibitor (a nitrification inhibitor and / or a urease inhibitor) and amino acid, and the soil conditioner outer layer is composed of a soil conditioner outer layer. The soil conditioner outer layer is composed of a soil conditioner special for saline-alkali soil improvement. The method has the beneficial effects that (1) the pH value of the soil is adjusted and the soil structure is improved, so that the microbial flora can better adapt to the soil environment of the saline-alkali soil; and (2) the biochar and the isolating membrane effectively protect the activity of microorganisms, and the microorganisms are released when the pH value and the salt-based ions of the soil around the rhizosphere reach a lower level, so that the saline-alkaline obstacle of the growth of the microorganisms is overcome.

The present invention discloses a water quality monitoring system applied to sewage treatment, which relates to the technical field of sewage water quality monitoring. Before adding microorganisms, the present invention monitors and analyzes the uniformity of water quality, confirms the sewage treatment mode. During stratified treatment, it analyzes the microorganism combination plan and microorganism addition plan for sewage treatment at each depth layer. During overall treatment, it analyzes the microorganism combination plan and overall microorganism addition plan for sewage, and after adding microorganisms, it conducts water quality monitoring and then makes corresponding adjustments to ensure the unity and uniformity of the water quality treatment progress, which helps to maintain the stability of the microorganism growth environment, so that the microorganisms can continuously work efficiently in a relatively stable environment, improve the decomposition speed of pollutants in the sewage, ensure the stability of the effluent water quality, reduce the difficulty of subsequent microorganism control and regulation, improve the overall sewage treatment effect, and is not easy to cause microorganism waste, reducing the operation cost.

The invention discloses a soil microorganism carbon utilization efficiency determination method, and relates to the technical field of carbon utilization efficiency determination, and the method comprises soil sample pre-culture, multi-source 18O isotope gradient labeling, dynamic gas monitoring, microorganism respiration rate dynamic correction, and microorganism growth rate and carbon utilization efficiency acquisition. By labeling multi-source isotopes and quantifying contributions of different oxygen sources to microorganism DNA synthesis, underestimation of the carbon utilization efficiency caused by a traditional single labeling method is avoided, the problem of limitation existing in the current soil microorganism carbon utilization efficiency determination process is solved, the inhibition effect of too high local concentration on microorganism metabolism is reduced through gradient labeling, and the accuracy of soil microorganism carbon utilization efficiency determination is improved. The method has the advantages that marking uniformity is improved, multiple groups of soil samples are arranged, real-time change of microbial respiration is captured by means of dynamic monitoring, influence of isotope fractionation on carbon dioxide concentration calculation is eliminated, instantaneous errors of traditional static measurement are avoided, and accuracy of analysis results is improved to a certain extent.

The invention belongs to the technical field of soil remediation, and particularly relates to a multifunctional composite soil conditioner and a preparation method thereof. The composition comprises a component A and a component B, the component A is prepared from the following components in parts by weight: 35 to 50 parts of modified charcoal, 8 to 12 parts of diatomite, 3 to 5 parts of bentonite, 13 to 18 parts of chitosan modified humic acid and 4 to 6 parts of nano silicon dioxide; the component B is prepared from the following components: 8 to 12 parts of lime powder, 5 to 10 parts of polyvinyl alcohol, 5 to 10 parts of polylactic acid, 5 to 8 parts of nanocrystalline cellulose, 6 to 10 parts of plant extract and 5 to 8 parts of sodium alginate. According to the invention, a stable three-dimensional adsorption network is constructed by using biochar and a nano material, so that the heavy metal fixing capability is enhanced; the chitosan modified humic acid promotes the growth of beneficial microorganisms and improves the self-repairing capability of the soil; slow-release lime powder ensures stable adjustment of the pH value, and polyvinyl alcohol and the like form a protective layer to ensure a long-acting effect.

The invention relates to the technical field of microbiological detection and image detection, and provides a microwell plate microbiological growth state detection method, system and equipment based on artificial intelligence. The system comprises an image acquisition module, an image segmentation module, a data set construction module, an image data enhancement module, an image classification module, a classification result determination module and a reasoning module, and is used for detecting whether bacteria grow in the microwell plate; or the system comprises an image regression module and a regression error evaluation module and is used for detecting the biomass of the thalli. According to the invention, a self-built photographing device can be adopted to realize on-site direct automatic detection without sampling, so that high-throughput automatic accurate analysis of the growth state of microorganisms in the microwell plate can be conveniently and quickly realized, experiment consumables and biological samples can be saved, the contamination risk can be reduced, and the experiment efficiency can be remarkably improved. Therefore, the working intensity of detection personnel is reduced, and the method has wide application value. Meanwhile, the complexity of detecting the growth state of the microorganisms in the pore plate is reduced, and popularization and application are facilitated.

The invention relates to a method for solidifying aeolian sand and resisting wind erosion by combining microorganisms with sugar calcium, which is characterized in that a sandy soil body is more sufficiently and efficiently solidified by utilizing a synergistic effect mechanism of combining MICP with sugar calcium, so that the purposes of enhancing the strength of the sandy soil body and resisting natural environment erosion are achieved; in the process of solidifying the sandy soil by combining sugar and calcium with MICP, the sugar and calcium can promote the growth of microorganisms, regulate the reaction environment and contribute to the generation of calcium carbonate precipitates, so that the sandy soil particles play a role in cementation, and meanwhile, the sugar and calcium are dissolved in water to release viscous substances, so that the generated calcium carbonate precipitates are more uniform, and the quality of the soil is improved. The effect of solidifying a sandy soil body is more obvious; the method adopts green and environment-friendly materials, is suitable for reinforcing a large-area sandy soil area, has a very remarkable curing effect, has the excellent characteristic of resisting natural environment erosion, and is widely applied to the fields of sandy soil curing, protection and the like.

The present invention provides antimicrobial compositions comprising an Aspergillus fermentate and methods of making said antimicrobial compositions. Also provided are products comprising the antimicrobial compositions and methods of using the antimicrobial compositions to prevent the growth of a microbe.

The invention relates to preparation and technical methods of a composite saline soil modifier. The modifier is prepared from 30-40 parts of charcoal, 8-10 parts of oxalic acid, 15-20 parts of desulfurized gypsum, 3-6 parts of wood vinegar, 1-2 parts of microbial agent fermentation liquor and 30-40 parts of other auxiliary components. Wherein the microbial agent fermentation liquor is prepared by fermenting bacillus subtilis, the biochar is prepared by pyrolyzing rice husk at 600 DEG C for 2 hours, and the desulfurized gypsum is prepared by wet desulfurization; the preparation method of the modifier comprises the following steps: uniformly mixing the raw materials in proportion, drying until the water content is lower than 10%, crushing and packaging. During application, the fertilizer is uniformly applied to salinized soil according to the dosage of 100 kg / mu, the ploughing depth is 20-30 cm, and the soil is kept moist. Experimental results show that the modifier can significantly reduce the pH value of soil, increase the number of water-stable aggregates, increase the water retention capacity of soil, promote the growth of soil microorganisms and enhance the salt resistance of crops, and has a significant improvement effect on mild to moderate salinized soil.