276 results about "Psicose" patented technology

The invention discloses a water-based soldering flux for a photovoltaic N-type battery 0BB assembly and a preparation method of the water-based soldering flux, and belongs to the technical field of soldering flux. The soldering flux is prepared from the following raw materials in parts by weight: 30-60 parts of deionized water, 30-50 parts of an alcohol solvent, 2-8 parts of a high-boiling-point solvent, 1-4 parts of organic acid, 0.1-1 part of a surfactant, 0.1-1 part of an antioxidant, 0.1-1 part of a corrosion inhibitor, 0-1 part of psicose and 0-1 part of trimethylolpropane tricaprylate. According to the invention, the problem of negative influence on tension of thermosetting adhesive and alloying of a fine grid when a traditional soldering flux is applied to a photovoltaic N-type battery 0BB assembly is solved.

The invention discloses a D-psicose 6-phosphate phosphatase mutant and application of the D-psicose 6-phosphate phosphatase mutant in preparation of psicose, and belongs to the technical field of enzyme engineering. According to the invention, site-directed mutagenesis is carried out on an amino acid sequence of D-psicose 6-phosphate phosphatase A6PP, and a combined mutant I21V / Y73F / R115M / T180V / L203I / Q193E is constructed, so that the substrate specificity, the thermal stability and the catalytic activity of the combined mutant are all remarkably improved. The invention further provides application of the combined mutant in preparation of psicose through multi-enzyme cascade catalysis, 150 g / L of maltodextrin can be converted into 97.7 g / L of D-psicose, the conversion rate reaches 65.1%, and the application prospect of the combined mutant in the field of food is widened.

The invention discloses L-rhamnose isomerase and application thereof, and belongs to the technical field of genetic engineering. The amino acid sequence of the L-rhamnose isomerase is obtained by performing the following mutation on an amino acid sequence as shown in SEQ ID NO.1: the 258th amino acid is mutated from D to Q. Compared with the prior art, the L-rhamnose isomerase mutant D258Q is obtained by mutating wild type L-rhamnose isomerase, and compared with the wild type L-rhamnose isomerase, the L-rhamnose isomerase mutant D258Q is higher in enzyme activity and longer in half-life period, so that when the L-rhamnose isomerase mutant D258Q is used for catalyzing the conversion of D-psicose into D-allose, the L-rhamnose isomerase mutant D258Q has the advantages that the enzyme activity is higher, and the half-life period is longer; the catalytic capability is stronger and more durable, and the conversion efficiency of the reaction and the yield of D-allose are improved.

A food composition includes allulose having a concentration of about 60% to about 70% by weight, a cocoa product having a concentration of about 20% to about 30% by weight, and at least one natural plant resin having a concentration of about 0.37% to about 2.19% by weight. The at least one natural plant resin may be mastic gum and / or mastic powder. The food composition may further include a combination of plant-derived biominerals and / or antioxidants as active ingredients. A confectionary product made with the food composition is also disclosed.

The invention belongs to the technical field of gene engineering and protein engineering, and particularly relates to thermal-stable D-psicose 3-epimerase as well as a preparation method and application thereof. According to the invention, a series of D-psicose 3-epimerase DAE mutants are constructed, and six mutants which not only can retain good catalytic activity, but also have high thermal stability are obtained through screening. The catalytic temperature of the three mutants A19D / A223P / V241I is increased by 25 DEG C compared with that of a wild type, while the three mutants A19D / A223P / V241I still show the optimal thermal stability, the catalytic activity of the three mutants is improved by 17.0%, and the three mutants show good industrial suitability. The D-psicose 3-epimerase DAE mutant with improved thermal stability provided by the invention is beneficial to improving the production efficiency of the DAE in industrial application of D-psicose, and provides powerful industrial enzyme guarantee for large-scale and low-cost preparation of D-psicose.

A method for improving gut microbiota population in a subject by administering a composition comprising allulose, erythritol or a combination thereof. A method for increasing one or more colonic bacterial populations from the families Lachnospiraceae or Eubacteriaceae by administering a composition comprising allulose, erythritol or a combination thereof. A method for preventing, treating or ameliorating a disease in a subject by administering a composition comprising allulose, erythritol or a combination thereof. The disease may be one or more of metabolic syndrome, obesity, type 2 diabetes, insulin resistance, hyperlipoproteinemia, hyperuricemia, hepatic steatosis, hypercholesterolemia, hypertriglyceridemia, irritable bowel syndrome (IBS), colon cancer, allergy, non-alcoholic fatty liver disease, inflammatory bowel disease, cardiovascular disease, or inflammation. The bacterial population may be the species Anaerostipes hadrus, Blautia obeum, Anaerobutyricum hallii, Eubacterium rectale (Agathobacter rectalis), Eubacterium callanderi, Eubacterium limosum and Eubacterium maltosivorans. A composition comprising allulose, erythritol or a combination thereof and one or more colonic bacterial populations from the families Lachnospiraceae or Eubacteriaceae is also provided. The composition may in the form of a food composition.

The invention provides a healthy food, in particular to a hypoglycemic composition based on homology of medicine and food and gradient fermentation type prebiotics, which is a dry powder preparation and comprises a component A and a component B, the component A is a medicinal and edible component, and the component B is a prebiotic component; the component A is prepared from the following components in parts by mass: 2 to 15 parts of folium mori extract, 2 to 10 parts of bitter gourd extract, 2 to 20 parts of rhizoma dioscoreae extract, 1 to 10 parts of radix puerariae extract, 2 to 10 parts of fructus lycii extract, 0.5 to 5 parts of radix polygonati officinalis extract and 0.5 to 5 parts of rhizoma polygonati extract; the component B is prepared from 20 to 50 parts of resistant dextrin, 5 to 15 parts of xylooligosaccharide, 3 to 10 parts of inulin and 35 to 50 parts of D-psicose. The hypoglycemic activity of the traditional medicinal and edible materials is organically combined with the gradient fermentation type prebiotics, so that the hypoglycemic effect is realized by synergism of multiple action mechanisms, and the problems of single hypoglycemic mechanism and non-ideal blood sugar regulation effect of the existing product are solved.

The invention discloses a method for efficiently synthesizing D-psicose from cane sugar by escherichia coli through double PTS transfer. E.coliJM109 (DE3) is used as a chassis host bacterium, and a D-psicose synthesis path based on a sucrose PTS transport system is established through co-expression of alsE, a6PP, scrA and scrB genes; by introducing the glpX [K29A] gene, a carbon source is driven to flow from a D-fructose PTS transport system to a D-psicose synthesis path; through knockout of pfkA and rpiA genes and inhibition of expression of fbaA genes, an endogenous glycolysis pathway and a phosphopentose pathway are adjusted, and the carbon flow entering tricarboxylic acid circulation is adjusted, and finally, the purpose that the D-psicose is efficiently synthesized from sucrose by escherichia coli through double PTS transport is finally achieved. Genetic engineering modification is carried out on wild escherichia coli, D-psicose is efficiently synthesized from sucrose by using a sucrose PTS and fructose PTS system, and a new scheme is provided for synthesis of D-psicose.

The invention provides a group of recombinant Escherichia coli for forming D-psicose. The recombinant Escherichia coli comprises the following multiple modifications: (a) introducing and expressing a dTDP-glucose-3-epimerase gene, an NDP glycohydrolase gene, an allose-1 phosphate-isomerase gene and an acid phosphatase gene; and (b) enhancing the expression of the glucose phosphate mutase gene, the expression of the glucose 1-phosphate thymine transferase 1 gene and the expression of the glucose 1-phosphate thymine transferase 2 gene. The recombinant Escherichia coli capable of producing D-psicose at high yield is prepared, the yield of by-products is reduced while the yield of a target object is increased, the bottleneck problem in industrial production is solved, and the recombinant Escherichia coli can be used for mass production of D-psicose.

The present disclosure relates to microorganisms for psicose biosynthesis. Methods of producing the disclosed microorganisms and methods of producing psicose are also provided.

The invention relates to the technical field of beverages, and particularly discloses a high-protein beverage containing psicose and a preparation method of the high-protein beverage. The high-protein beverage containing psicose is prepared from the following raw materials in percentage by mass: 13 to 17 percent of supplementary protein, 4 to 5 percent of an emulsifier, 3.10 to 3.14 percent of a flavoring agent, 0.02 to 0.06 percent of a thickening agent, 0.005 to 0.015 percent of a stabilizer, 0.008 to 0.01 percent of a coloring agent, 0.04 to 0.08 percent of an enzyme preparation and the balance of water, the flavoring agent is prepared from D-psicose, erythritol, sucralose and stevioside. The D-psicose in the flavoring agent is extremely low in calorie, does not cause increase of blood sugar and insulin, and has a unique taste, and the erythritol, the sucralose and the stevioside are matched, so that the taste of sucrose is reduced on the premise of low calorie, and the problem of bitter taste of a sugar substitute beverage is solved, so that the flavor of the protein beverage is improved.

The invention relates to the technical field of gene engineering, in particular to a D-psicose-3-epimerase mutant and application of the D-psicose-3-epimerase mutant in synthesis of psicose. According to the present invention, the 59th site, the 106th site and the 188th site of the wild type D-psicose-3-epimerase derived from Brevibacillus thermophilus (Brevibacillus thermophilus) are subjected to simultaneous mutation to obtain the D-psicose-3-epimerase mutant, such that the efficiency of producing D-psicose by using fructose as the raw material is significantly improved, the reaction of catalyzing 500 g / L fructose to produce D-psicose is performed for 4 h, and the yield of the D-psicose is significantly improved; the conversion rate is up to 36.5%; according to the present invention, the coexpression binding enzyme strain is constructed by coupling glucose isomerase, such that the intermediate transfer efficiency and the enzyme stability are improved, the reaction for producing D-psicose through catalysis of 500 g / L glucose is performed for 6 h, and the conversion rate can achieve 30.5%; and the activity of the immobilized enzyme can still be kept at 80% after the immobilized enzyme is repeatedly used for 10 batches.

The invention belongs to the technical field of bioengineering and enzyme engineering, and discloses a strain for producing D-psicose-3-epimerase, thallus immobilized particles and a catalytic method, the strain is classified and named as Bacillus halotolans, the strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on December 26, 2022, the preservation number is CGMCC No.26285, and the preservation number is CGMCC No.26285. The invention further discloses a preparation method of the D-psicose-3-epimerase for producing the D-psicose-3-epimerase for producing the D-psicose-3-epimerase for producing the D-psicose-3-epimerase for producing the D-psicose-3-epimerase. The thalli are loaded on the supporting carrier through the cross-linking agent, slow drying is achieved in the cross-linking drying process, and the obtained thalli immobilized particles have the advantages of being large in mechanical strength and insoluble in water.

The invention discloses a method for synthesizing D-psicose by multi-enzyme immobilized cascade catalysis of sucrose, and belongs to the technical field of food production. The method provided by the invention comprises the following steps: respectively immobilizing sucrose invertase, glucose isomerase and epimerase by utilizing protein affinity immobilization purification magnetic beads to prepare immobilized enzyme, carrying out enzymatic reaction by taking sucrose as a substrate, and converting the sucrose into D-psicose step by step. Experimental results show that the method provided by the invention can effectively convert cane sugar, glucose and fructose which are easy to absorb into D-psicose which is difficult to absorb; after the enzymatic reaction, the immobilized enzyme can be effectively recovered through rapid separation and can be reused, so that the production cost is saved. The invention provides a low-cost and high-efficiency production method for the production of D-psicose, and the method has a wide application prospect.

The present invention discloses a low GI blackcurrant jam and a preparation method thereof, and relates to the technical field of food processing, the low GI blackcurrant jam comprises the following components by mass: 50-70 parts of blackcurrant pulp, 15-35 parts of a composite sugar source, 0.3-1.5 parts of a compound thickener, 0.1-0.7 part of an acidity regulator, and 5-15 parts of water; the composite sugar source consists of psicose, inulin and resistant dextrin; the compound thickening agent is prepared from apple gum, konjac gum and xanthan gum; the preparation process comprises the following steps: step-by-step sugar melting under a mild condition, colloid pre-dispersing gel melting, fruit pulp mixing, pasteurization, acid regulation and homogenization, sterilization after filling and the like. Through the cooperation of a formula system and a whole-process mild temperature control process, a stable three-dimensional gel network is constructed while the low GI value and the high vitamin C retention rate of the product are achieved, water bleeding and layering are effectively prevented, and therefore the jam product with the healthy property and the excellent texture is obtained.

The application discloses a D-allulose 3-epimerase mutant, a preparation method and application thereof, and relates to the fields of genetic engineering and enzyme engineering. The amino acid sequence of the D-allulose 3-epimerase mutant is shown as SEQ ID NO. 1. The specific enzyme activity of the D-allulose 3-epimerase mutant provided by the application is increased from 10.21 U / mg of a control (before mutation) to 15.29 U / mg at 80 DEG C and pH 6.0; and the equilibrium conversion rate is increased from 29.98% of the control (before mutation) to 36.26% when the substrate is 700 g / L of fructose. The D-allulose 3-epimerase mutant provided by the application has higher catalytic activity, and has a very broad application prospect in the efficient production and preparation of D-allulose.

This invention discloses a compound peptide nutrient with fat-reducing effects, belonging to the field of health food technology. Specifically, it relates to a VQPIPY peptide or a product containing VQPIPY peptide in the preparation of lipid-lowering drugs or health products, or in the preparation of drugs for treating and / or alleviating alcoholic liver damage. This invention also provides a meal replacement powder containing VQPIPY peptide, shown as a compound of whey powder, milk powder, oats, spinach powder, allulose, and camel milk peptide in a specific ratio. Through the synergistic effect of its components, this product achieves multiple effects such as providing satiety, inhibiting blood sugar, and lowering blood lipids. It is characterized by balanced nutrition, safety, effectiveness, and good taste, and can be used for weight management and daily health maintenance.

Disclosed herein are formulations (including sweet proteins and rare sugars) for improving the sweetness of low glycemic index rare sugars without altering the texture and flavor of the rare sugars and methods for improving the sweetness of rare sugars. More specifically, the present invention discloses methods for improving the sweetness of rare sugars without altering the sweetness profile, texture and flavor of the primary sugars. The invention also discloses the addition of Brazitame protein with liquid rare sugars at different temperatures during the synthesis of the rare sugars, before crystallization or before or after caramelization of the rare sugars, to increase sweetness. The Brazilian sweet protein increases the sweetness of rare sugars, but is not limited to D-psicose, D-tagatose, D-allose, isomaltulose, trehalose, sorbose, pinose, leucobiose and the like. The invention also provides for the evaluation of non-nutritional sweeteners (such as aspartame, acesulfame, sodium cyclamate, neotame, saccharin, sucralose, stevioside, etc.), as each has a limitation of taste quality if used with rare sugars. However, the use of Brazilian sweet protein does not alter the flavor and texture of rare sugars, and the taste profile matches sucrose.

ABSTRACT The technology described in this specification relates to a dry sweetener composition and methods for preparing the sweetener. The sweetener may be a single sweetener, for example, allulose, or it may be a mixture of sweeteners. The method comprises providing a concentrated syrup comprising a sweetener and a seed solid comprising a carbohydrate, and contacting the syrup with the seed solid to form a composite composition; and drying the composite composition under conditions to obtain the solid sweetener composition, wherein at least a portion of the solid sweetener is reintroduced into the process to act as a seed solid.

The present invention produces fermented kombucha beverages with reduced alcohol content by utilizing allulose or analogues thereof as the primary fermentable sugar. These rare sugars limit ethanol production during fermentation while preserving flavor, probiotic benefits, and acidity. The invention further encompasses the incorporation of Kratom (Mitragyna speciosa) extracts or alkaloids into the fermentation process or final product, enabling a low-alcohol kombucha with potential enhanced wellness properties. The resulting beverage maintains an alcohol content below 0.5% by volume, making it suitable for non-alcoholic labeling and broader consumer appeal. The invention is preferably a bottled beverage.

The invention belongs to the technical field of gene engineering, and in particular relates to a D-psicose-3-epimerase mutant subjected to Spytag / SpyCatcher cyclization modification and an application of the D-psicose-3-epimerase mutant. The preparation method comprises the following steps: carrying out multi-site simultaneous mutation on 109th aspartic acid and 160th serine of wild type D-psicose-3-epimerase to obtain a mutation intermediate, respectively connecting a Spytag tag and a SpyCatcher tag to the N end and the C end of the mutation intermediate, and carrying out cyclization modification, so as to obtain the wild type D-psicose-3-epimerase, wherein the Spytag tag and the SpyCatcher tag are respectively connected to the N end and the C end of the mutation intermediate; when fructose is used as a raw material to produce D-psicose and host bacteria are escherichia coli, the reaction of catalyzing 600 g / L fructose to produce D-psicose is performed for 4 h, and the conversion rate can reach 35.7%; when the host bacteria are bacillus subtilis, the reaction for catalyzing 500 g / L fructose to produce D-psicose is performed for 4 h, and the conversion rate reaches 36.5%; compared with a wild type D-psicose-3-epimerase strain of bacillus subtilis, the conversion rate of the strain is improved by 13.5%.

The present disclosure relates to a chocolate composition comprising allulose and at least one ingredient from a cocoa bean selected from the group consisting of cocoa mass, cocoa powder, and cocoa butter; and a method of manufacturing such chocolate; in particular the present disclosure relates to a chocolate composition prepared by said method, and confectionary products comprising said chocolate composition.

Non-dried allulose crystals are dried in a temperature treating step (b) in a drying apparatus at a temperature of about 25° C. to about 70° C., and in a conditioning step (c). The temperature treating step (b) may be carried out (b1) at atmospheric pressure and a residence time of about 5 minutes to about 5 hours. The temperature treating step (b) may be carried out (b2) under reduced pressure and constant temperature and a residence time from about 40 minutes to about 5 hours. The conditioning step (c) may be carried out (c1) over a period of about 30 minutes to 7 hours at a temperature from about 40° C. to about 70° C. The conditioning step (c) may be carried out (c2) over a period of about 15 minutes to about 90 hours at about 30 to about 60% relative humidity, and a temperature of about 25 to about 40° C.

Disclosed herein are genetically engineered Kluyveromyces sp. cells capable of producing D-allulose. The genetically engineered Kluyveromyces sp. cells comprise an exogenous polynucleotide sequence encoding an allulose-6-phosphate 3-epimerase enzyme at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or100% identical to at least one of SEQ ID NOs:249-256, 258, and 259; and an exogenous polynucleotide sequence encoding an allulose-6-phosphate phosphatase enzyme at least 70%, at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 99%, or 100% identical to at least one of SEQ ID NOs:87, 89, 190, 123, 105, 107, 115, 83, 95, 113, 117, 119, 121, 127, 131, 137, 145, 169, 173, 179, and 183.

The present invention discloses a D - psicose 3 - epimerase, its recombinant vector, strain and application, belonging to the technical field of functional enzymes. The present invention firstly provides a D - psicose 3 - epimerase derived from halotolerant Bacillus sp. ( Halotolerant Bacillus ), SYNY - 019. The protein sequence of the D - psicose - 3 - epimerase is as shown in SEQ ID No.1, and the nucleotide sequence obtained after codon optimization of this protein sequence is as shown in SEQ ID No.2. This D - psicose 3 - epimerase can be used for efficiently catalyzing the synthesis of D - psicose from D - fructose.

One embodiment of the present invention provides a method for purifying a psicose-containing solution, the method comprising the step of passing a psicose-containing solution through an ion exchange resin column filled with a mixed bed ion exchange resin to obtain an ion-purified psicose-containing solution. In one embodiment of the method for purifying the psicose-containing solution, the mixed bed ion exchange resin is a mixture of strongly acidic cation exchange resin and weakly alkaline anion exchange resin, and the weakly alkaline anion exchange resin constituting the mixed bed ion exchange resin has a quaternary ammonium exchange capacity fraction of 0-10%. When the method for purifying the psicose-containing solution according to the present invention is used, not only the conductivity of the psicose-containing solution can be reduced to an acceptable level, but also psicose loss can be minimized by inhibiting conversion of psicose into psicose or other substances due to functional groups of an ion exchange resin. Therefore, the method for purifying the psicose-containing solution according to the present invention is suitable for mass production of high-quality psicose.

The proposal proposes a crystalline allulose with a defined particle size distribution, a process for its production, and its use. This new allulose grade is characterized by its improved shelf life and improved sensory and flavor properties of finished products made with it.

The present invention relates to a psicose syrup with improved properties.

The present application relates to allulose crystals, which have an X-ray powder diffraction pattern including peaks at 2θ diffraction angles of 18.8±0.5°, 15.2±0.5°, and 19.5±0.5° in X-ray powder diffraction (XRD) analysis, a sweetener composition containing allulose crystals, and a method for producing allulose crystals.

The present application relates to an allulose-producing microorganism and a method for producing allulose using the same.