50results about How to "Simple purification process" patented technology

The invention provides a method for preparing vanadium pentoxide from iron-containing ammonium metavanadate, which comprises the following steps: S1) mixing iron-containing ammonium metavanadate with water to obtain ammonium metavanadate slurry; s2) mixing the ammonium metavanadate slurry with a complexing agent, adjusting the pH value to 1.5-2.5, carrying out iron dissolving reaction, and carrying out solid-liquid separation to obtain ammonium polyvanadate; 3, ammonium polyvanadate is calcined, and vanadium pentoxide is obtained.According to the method for preparing vanadium pentoxide from iron-containing ammonium metavanadate, the technical thought of acid dissolution-complexing iron removal is provided, the content of iron in vanadium pentoxide is effectively reduced, the process is simple, and the process cost is low.

The invention belongs to the technical field of compound synthesis, and particularly relates to a synthesis method of AEA-AEA. Triethylene glycol is adopted as a starting material, in the first step, p-toluenesulfonate with high leaving property is formed through TsCl, then nucleophilic substitution is performed, and a product 2 is obtained; the preparation method comprises the following steps: preparing a product 3, oxidizing by adopting TEMPO capable of being industrially produced, removing Pht through quantitative hydrazine hydrate to obtain a product 4, esterifying and extracting to obtain a product 5, acylating through the product 3 to obtain amide, washing, extracting and pulping to obtain an esterified product 6; and finally, quantitatively removing Pht, hydrolyzing and pulping to obtain a pure product. According to the method, complex operation steps of using metal hydride and alkylating column passing are avoided, process production can be realized, and the product yield and purity are relatively high.

The invention discloses a method for preparing recombinant human copper-zinc superoxide dismutase. The method comprises the following steps: (a) designing and synthesizing a gene sequence for coding the recombinant human copper-zinc superoxide dismutase; (b) cloning the gene sequence into an expression vector to construct a recombinant expression vector; (c) transforming the recombinant expression vector into an expression host cell to obtain a recombinant strain; (d) inducing the expression host cell to express the recombinant human copper-zinc superoxide dismutase under proper conditions; and (e) collecting an expression product, and carrying out multi-step purification to obtain the high-purity recombinant human copper-zinc superoxide dismutase. According to the superoxide dismutase provided by the invention, the expression quantity and activity of recombinant protein are remarkably improved, the purification process is simplified, the production cost is reduced, and meanwhile, the high-quality standard of a product is ensured.

The invention discloses a light salt preparation integrated device and method, and an electrolytic bath waste heat cold electricity light salt co-production system and method comprising the light salt preparation integrated device. The light salt preparation integrated device comprises an upper-stage light salt separation module and a lower-stage condensation light preparation module. The electrolytic bath waste heat cold electricity light salt co-production system comprises a power module, a cascade refrigeration module, a light salt preparation module and a hydrogen purification module. The light salt preparation integrated device disclosed by the invention can be used for preparing light salt by utilizing a freezing desalination method, and the supplement process and the cost of the electrolyte are simplified. And the electrolytic bath waste heat cold electricity light salt co-production system can drive the system to perform multi-effect co-production and purify hydrogen through coupling linkage among different modules by taking waste heat of seawater electrolysis hydrogen production as a heat source, so that the hydrogen purification process is simplified, and the overall cost of seawater electrolysis hydrogen production is reduced.

The invention provides an oxytetracycline injection and a preparation method thereof, and belongs to the technical field of medicine preparation, the preparation method comprises the following steps: uniformly mixing 35-50 parts by weight of polyethylene glycol 400 and water, sequentially adding 0.4-0.6 part by weight of sodium formaldehyde sulfoxylate and 1.6-1.8 parts by weight of magnesium oxide, heating, adding 18-22 parts by weight of oxytetracycline, carrying out a complex reaction, adding monoethanolamine to adjust the pH value to 8.4-8.6, and uniformly stirring to obtain the oxytetracycline injection. And fixing the volume and filtering to obtain the oxytetracycline injection. The oxytetracycline injection prepared from the oxytetracycline can effectively improve the stability and the drug effect, prolong the half-life period of the drug and reduce the irritation.

The invention relates to a preparation method of a suvorexant intermediate, and belongs to the field of medical compounds. According to the invention, 2-bromo-5-methyl benzoic acid with low price is used as a reaction raw material, and the Ullmann coupling reaction can be carried out at a lower temperature by adding amine, amino acid or oxamide ligands, so that the yield and selectivity of the product are greatly improved, and the ratio of the product to a regioisomer is remarkably increased. The route is low in cost, high in yield, simple and convenient to operate, mild in reaction condition and safe, and better meets the requirements of industrial production.

The utility model relates to the field of petrochemical engineering, and particularly discloses a sulfolane purifying device, which comprises a mixing tank and a separator, the mixing tank is used for mixing sulfolane and a solid purifying agent, the separator is used for separating sulfolane and the solid purifying agent, and the bottom of the mixing tank is connected with the separator. The separator is provided with a distribution disc, and the distribution disc comprises a plurality of distribution pipes which are communicated with one another. Each distribution pipe is independently provided with one or more connectors, each connector is connected with a filter grid, and the filter grids are used for filtering solid purifying agents. The device is further provided with a suction pump, one of the distribution pipes is connected with an inlet of the suction pump, and the suction pump is used for pumping out materials in the separator. Through the device, colored substances, acidic substances and hydrocarbon substances in the sulfolane can be purified and removed, so that the extraction performance of the sulfolane is recovered.

The present invention belongs to the field of bioengineering technology, and discloses a preparation method and application of an amniotic membrane extract. Fresh amniotic membrane is disinfected, cleaned, and dried in sequence, and then crushed in an ice bath. Then, the crushed liquid is centrifuged and filtered to obtain the amniotic membrane extract. The dry matter of the amniotic membrane extract meets the following requirements: total protein content ≥78.02%, total sugar content ≥10.87%, fat content ≤3.53%, and ignition residue content ≤1.64%. The present invention improves the extraction efficiency of total protein and total sugar in the amniotic membrane extract by changing the existing production process of frozen crushing or liquid crushing, crushing the amniotic membrane in an ice-water mixture, and combining the control of process parameters such as crushing, which is beneficial to the promotion and application of the amniotic membrane extract in the preparation of ocular preparations such as ocular surface lubricating liquid, and improves the clinical use effect. In addition, the amniotic membrane extract can also be used as a functional component of biological amniotic membrane preservation liquid and combined with amniotic membrane covering surgery for the treatment of ocular surface diseases.

The invention belongs to the technical field of synthesis of lithium difluoro (oxalato) borate, and particularly relates to a preparation method and application of lithium difluoro (oxalato) borate. The preparation method of lithium difluoro (oxalato) borate comprises the following steps: mixing a boron fluoride-containing complex, an oxalate-containing compound and a solvent, and reacting to obtain a primary reaction solution; mixing oxalic acid, a silane compound and the first-stage reaction liquid, and reacting to obtain a second-stage reaction liquid; and concentrating the secondary reaction liquid, adding a solvent, heating, cooling and separating out to obtain lithium difluoro (oxalato) borate. The primary reaction liquid containing lithium difluoro (oxalato) borate and lithium tetrafluoroborate is obtained through specific raw materials and processes, then oxalic acid and the silane compound are added, the by-product lithium tetrafluoroborate is converted into lithium difluoro (oxalato) borate, separation operation of lithium difluoro (oxalato) borate and lithium tetrafluoroborate is not needed, the process is simple, and the cost is low. The yield and the purity of the lithium difluoro (oxalato) borate can be improved.

The invention discloses a method for preparing an elderberry extract, and relates to the technical field of plant extraction; the method comprises the following steps: S1, extracting an elderberry raw material by countercurrent extraction to obtain an extract; S2, concentrating the extract to obtain a concentrated solution; S3, mixing the concentrated solution, a glycosyl donor and an enzyme, and then glycosylating to obtain a glycosylated product; S4, passing the glycosylated product through a macroporous resin column, eluting with water and an ethanol aqueous solution with a volume fraction of 50% to 70% in sequence, and collecting the ethanol aqueous solution eluate; S5, microfiltration and nanofiltration of the ethanol aqueous solution eluate in sequence, collecting the filtrate; and concentrating the filtrate to dryness. The preparation method of the invention has simple steps and low cost; and an anthocyanin extract with a mass content of ≥65% is also prepared.

The scheme relates to a method for producing soluble human acetaldehyde dehydrogenase 2 in escherichia coli in the field of enzyme engineering, and the method comprises the following steps: firstly, inserting a human ALDH2 gene into a pET30a (+) plasmid through Nde I and Xho I restriction enzyme cutting sites to form a recombinant expression vector; then, transforming the recombinant vector into escherichia coli by utilizing a heat shock method, and screening out recombinant engineering bacteria which are correctly sequenced; and finally, inoculating and culturing the engineering bacteria under proper conditions, adding lactose as an inducer (the final concentration is 0.2-0.3 mM), inducing for 4-8 hours under the conditions of 25-35 DEG C and 100r / min, and then collecting thalli. The thalli are suspended by a buffer solution, treated by a cell crusher and centrifuged to obtain crude enzyme, and the crude enzyme is further purified to obtain the soluble recombinant human-derived ALDH2. According to the method disclosed by the invention, the production of the soluble humanized acetaldehyde dehydrogenase 2 from the escherichia coli is realized.

The invention belongs to the technical field of biomacromolecular structure analysis, and particularly relates to a compound system and application thereof in small molecular weight protein cryoelectron microscope structure analysis. In particular, the present invention provides a composite system named Trimbdy, which greatly enhances the overall rigidity and stability of the composite. By utilizing the Trimbdy system provided by the invention, the atomic resolution (less than or equal to 3) cryoelectron microscope structures of a plurality of proteins with the molecular weight of less than 50 kDa are successfully analyzed, and the potential of serving as a simple, universal and efficient tool is proved, so that the Trimbdy system has a good practical application value.

The invention discloses a benzothiazole non-condensed ring A-D-A '-D-A type organic solar cell acceptor material and a preparation method and application thereof, a benzothiazole unit is used as a central nuclear electron withdrawing unit (A-unit), and then the benzothiazole unit, the central nuclear electron withdrawing unit (A-unit), an electron donating unit (D-unit) and a terminal group are synthesized into the organic solar cell acceptor material with an A-D-A'-D-A structure. Wherein benzothiazole as an asymmetric central core structure can enhance the dipole moment of molecules, optimize the accumulation among the molecules and improve the blending property of a donor and an acceptor, and the characteristics can jointly promote charge transfer to inhibit the bimolecular recombination condition. The molecular morphology is regulated and controlled by connecting different structural groups to a top branched chain, and meanwhile, different atoms can be introduced into a benzene ring to regulate and control the chemical energy level. In addition, the polymer also has the characteristics that the aggregation behavior and the light absorption range of molecules can be adjusted by accessing different terminal groups. The benzothiazole-based non-fused ring A-D-A '-D-A type acceptor material provided by the invention is few in synthesis and preparation steps, simple in purification process and high in yield.

The application discloses an engineered exosome targeting human urokinase-type plasminogen activator receptor uPAR and a preparation method and application thereof. Firstly, a high-affinity anti-human uPAR single-domain antibody VHH is prepared; then, a human umbilical cord mesenchymal stem cell engineering cell line expressing a VHH-PTGFRN fusion protein is constructed, and the engineered exosome is prepared and purified by combining three-dimensional hypoxic culture, chemical induction and size-exclusion chromatography technology. The engineered exosome comprises four comparative samples, wherein the core sample Exo^(VHH / miR) can simultaneously display the anti-uPAR VHH on the membrane surface to realize targeted delivery and enrich miR-193b-3p in the chamber to realize gene silencing, and the synergistic effect of the two can efficiently remove the uPAR high-expression senescent cells and inhibit the senescence-associated secretory phenotype SASP. The engineered exosome has the advantages of standardized preparation process, high yield and high purity, strong targeting, excellent curative effect and good safety, and can be used for preparing a treatment preparation for anti-aging and aging-related diseases, and has important clinical application value.

The application discloses a polypeptide salt conversion device and a use method thereof, and belongs to the technical field of polypeptide preparation; the device comprises an ion exchange salt conversion kettle and a detector, and the salt conversion kettle is provided with an upper cover, a pH meter, a stirring paddle, a lower sieve plate and a feed liquid inlet and outlet; the method comprises the following steps: filling ion exchange resin in the ion exchange salt conversion kettle, loading a polypeptide liquid after the polypeptide liquid is adjusted to below or above an isoelectric point, washing the polypeptide liquid with water to remove salt, then adding an acidic or alkaline ligand solution under the continuous stirring of the stirring paddle, stirring to make the resin fully contact with the ligand and promote the polypeptide to be quickly and uniformly converted, simultaneously monitoring the pH change of the system in real time by the pH meter to accurately control the reaction end point, and finally washing and removing to obtain the product. The detector monitors the cleaning of salt ions and the elution of a sample. The application can significantly improve the salt conversion efficiency and obtain a product with low impurity residue by stirring to strengthen mass transfer and pH linkage control.

This invention discloses a red pigment-producing Streptomyces spectabilis ( Streptomyces spectabilis This invention discloses a method and application for pigment extraction from *Streptomyces spectinomyces* S-13. The method involves fermenting *Streptomyces spectinomyces* S-13 with the addition of a surfactant to extract the pigment. This method releases intracellular pigments into the fermentation broth, significantly increasing pigment yield and simplifying the extraction and purification process. The red pigment produced by *Streptomyces spectinomyces* S-13 exhibits excellent stability, antioxidant activity, and antibacterial effects, making it suitable for applications in the printing and dyeing industry. The pigment extraction method described in this invention offers advantages such as short production cycle, low cost, safety, and environmental friendliness, overcoming the limitations of traditional microbial pigment production processes, including high extraction difficulty, low yield, and limited applicability.

The invention discloses natural neohesperidin and a separation and purification method thereof, and belongs to the technical field of natural product extraction.The high-purity neohesperidin is prepared from immature bitter oranges through smashing, extraction, coarse crystallization, organic solvent pulping, water pulping and recrystallization. The problems of complex process, long production period, high equipment requirement, high comprehensive production cost, high product ash content and the like in the existing neohesperidin separation and purification process are solved.

The application provides a recombinant human K14 keratin and a preparation method and application thereof, and relates to genetic engineering technology. The recombinant human K14 keratin has cell adhesion activity and comprises at least x continuous amino acid residues in SEQ ID NO. 10; wherein, 472 > x > 36. The recombinant human K14 keratin has higher cell proliferation activity and skin repair function, has low production cost, and is suitable for large-scale production.

The invention discloses a method for preparing silicon tetrafluoride by pyrolyzing sodium fluosilicate. The preparation method comprises the following steps: (1) respectively crushing and sieving sodium fluosilicate and an auxiliary agent, and activating at 250-620 DEG C; (2) mixing the activated sodium fluosilicate with an auxiliary agent, and performing pyrolytic reaction to obtain a silicon tetrafluoride crude product and a residual solid; and purifying the silicon tetrafluoride crude product to obtain a silicon tetrafluoride product. The method solves the problems of wall sticking and caking in the process of preparing silicon tetrafluoride through fluosilicate pyrolysis, and the decomposition efficiency is remarkably improved. Residual solids generated by pyrolysis can be recycled, and no fluorine-containing waste is generated in the process, so that the influence on the environment is effectively reduced.

The hard carbon raw material purification device comprises a feeder, an extraction chamber, a purification channel, an evaporation chamber and a recovery channel, the feeder is arranged above the extraction chamber; one end of the purification channel is arranged below the extraction chamber, the other end of the purification channel is connected with the top of the evaporation chamber, and a filter is arranged at one end, close to the evaporation chamber, of the purification channel; one end of the recovery channel is connected with the upper part of the evaporation chamber, and the other end of the recovery channel is connected with an extraction liquid inlet of the extraction chamber. The utility model provides a hard carbon raw material purification device which can effectively prepare a hard carbon raw material from low-ash coal, the raw material purified by the hard carbon raw material purification device can obtain a high-purity hard carbon material after being washed, evaporated and dried for multiple times and subjected to high-temperature carbonization treatment, and due to the integrated design scheme of the hard carbon raw material purification device, the high-purity hard carbon material can be obtained. The purification process of the hard carbon raw material is simplified, meanwhile, the chemical reagent for extracting the low-ash coal can be recycled, chemical pollution is reduced, and the purification cost is reduced.

The present application relates to a kind of 2-oxopentanedioic acid preparation method.The invention method is as follows: 2-methylene pentanedioic acid is dissolved in a certain amount of dimethyl carbonate, a certain amount of V2O5 / K2O / zeolite composite catalyst is added, air (or oxygen) is introduced at reflux temperature for a certain time.Oxidation is carried out.After reaction, the catalyst is removed by filtration, dimethyl carbonate is removed by vacuum distillation, 2-oxopentanedioic acid crude product is obtained, the yield is 98.5%, and refined 2-oxopentanedioic acid is obtained by recrystallization with water, the purity is 99.2%.The synthesis process of the present application is simple, the yield and product purity are high, the process cost is low, and it is an environment-friendly 2-oxopentanedioic acid synthesis method.

This invention belongs to the field of wet electronic chemical purification, specifically relating to a membrane chromatography filter membrane for removing charged ions from liquids and its preparation method, including the following steps: S1, preparing casting solutions: preparing positively charged layer casting solutions, neutral layer casting solutions, and negatively charged layer casting solutions separately; S2, using a three-layer die to sequentially extrude the positively charged layer casting solutions, neutral layer casting solutions, and negatively charged layer casting solutions onto a polyester film; S3, exposing the casting solutions under set temperature, humidity, and airflow conditions to allow for initial phase separation; S4, immersing the coated film in a pure water coagulation bath to complete phase separation and solidification; S5, rinsing, drying, and then winding to obtain the membrane chromatography filter membrane. By designing a special three-layer composite structure with a "positively charged layer-neutral layer-negative layer," the filter membrane can simultaneously adsorb anions and cations in the solution during a single filtration process, overcoming the limitation of traditional single-charge membranes that can only remove one type of ion, thus improving purification efficiency.

This invention relates to the field of cleaning solutions, specifically to a highly efficient hydroxylamine-free stripping and cleaning solution. The raw materials comprise the following components by mass fraction: 10-60% water, 10-50% water-soluble organic solvent (e.g., 10%-45%), 0.1-20% amine compound (e.g., 0.5%-10%), and 1-30% hydroxylamine substitute (e.g., 2%-20%), and a small amount of corrosion inhibitor, with the sum of the mass fractions of all components being 100%. The cleaning agent provided by this method exhibits good stability, prevents corrosion of Al metal during the cleaning process, has minimal negative impact on the conductivity of subsequent applications in device interconnects, and demonstrates good safety.

This invention discloses an immune-enhancing protein, its encoding gene, and its applications. The amino acid sequence of the immune-enhancing protein described in this application is shown in SEQ ID NO.1. This protein is derived from the C-terminal portion of the flagellated protein FliC from Salmonella Typhimurium. It not only retains the inherent immune-enhancing activity of flagellated proteins but also significantly reduces their immunogenicity. When applied to porcine circovirus type 2 subunit vaccines containing aluminum adjuvants, it can effectively enhance the immunogenicity of the vaccine, induce humoral and cellular immune responses, and significantly promote antigen-specific Th1 and Th2 immune responses. This is of great significance for improving the formulation of existing aluminum adjuvanted subunit vaccines and enhancing vaccine immunization efficacy.

The invention relates to the technical field of chemical materials, and discloses a preparation method of an anti-yellowing agent HN-130, which comprises the following steps: respectively dissolving hexamethylene diisocyanate and unsymmetrical dimethylhydrazine in a solvent to prepare a hexamethylene diisocyanate-solvent mixed solution and an unsymmetrical dimethylhydrazine-solvent mixed solution; dropwise adding the hexamethylene diisocyanate-solvent mixed solution into the unsymmetrical dimethylhydrazine-solvent mixed solution at a set temperature, and meanwhile, keeping constant temperature and continuously stirring until the hexamethylene diisocyanate-solvent mixed solution and the unsymmetrical dimethylhydrazine-solvent mixed solution are fully reacted; the method provided by the invention has low requirements on the purity of raw materials, wherein the purity of unsymmetrical dimethylhydrazine is greater than or equal to 90%; the method provided by the invention is simple in process and high in operability; the solvent can be recycled, and the solvent purification process is simple and wide in application range; compared with the existing synthesis process, the anti-yellowing agent HN-130 prepared by the method provided by the invention has the advantages of high yield, high product purity and low chromaticity.

The invention belongs to the technical field of biology, and particularly relates to preparation and application of a swine A type FMDV VP1 antigen and single factor positive serum. The sequence of the expression gene of the swine A type FMDV VP1 antigen is as shown in SEQ ID NO. 1. Through the integral technical scheme of combining sequence-driven conservative site screening, expression and immunological verification, the key problems of antigen mismatch, difficult recombinant protein expression, poor diagnostic reagent universality and the like in the prior art are solved; and the effectiveness and generalizability of the vaccine, the diagnostic reagent and the monitoring means in practical application are obviously enhanced.

The invention relates to the technical field of photoresist, in particular to a block copolymer based on poly (4-hydroxystyrene) as well as preparation and application thereof. The chemical structural formula of the poly (4-hydroxystyrene)-based segmented copolymer is as shown in a formula (A): # imgabs0 #; in the formula (A), R3 is H or CH3; when R1 is equal to CH3, R2 is equal to CH2CH3; when R1 is equal to CH2CH2CH3, R2 is equal to H; and x, y and z are integers. The block copolymer based on poly (4-hydroxystyrene) is synthesized and prepared by adopting an anionic polymerization method, and compared with a polymer obtained by a free radical polymerization process, the block copolymer has the advantages of high molecular weight repeatability, small molecular weight polydispersity coefficient (less than 1.2), high yield and simple purification process. The positive KrF photoresist composition based on the copolymer as the film-forming resin can effectively improve the photoetching resolution and sensitivity, and a photoetching pattern with good morphology is formed.

The present invention provides a recombinant genetically engineered bacterium of type A botulinum toxin and its preparation and application, belonging to the field of genetic engineering, the recombinant genetically engineered bacterium includes a pET‑28a vector, the pET‑28a vector includes a gene fragment 1 expressing a GST tag, a gene fragment 2 expressing a thrombin recognition site, and a gene fragment 3 expressing BoNT / A; the amino acid sequence of the GST is SEQ ID NO.1; the amino acid sequence of the thrombin recognition site is SEQ ID NO.2; the amino acid sequence of BoNT / A is SEQ ID NO.3. The recombinant genetically engineered bacterium provided by the present invention can produce type A botulinum toxin quickly and in large quantities. The present invention also optimizes the culture method of the recombinant genetically engineered bacterium, and increases the yield of type A botulinum toxin by adding an inducer. The present invention also optimizes the purification process of type A botulinum toxin, and prepares high-purity type A botulinum toxin.

This invention belongs to the field of compound synthesis technology, and specifically relates to a method and application for the synthesis of ethyl 7-bromobenzo[D][1,3]dioxane-4-carboxylate. The synthesis method of this invention includes the following steps: 2,3-dihydroxybenzoic acid undergoes esterification to obtain ethyl 2,3-dihydroxybenzoate; ethyl 2,3-dihydroxybenzoate is mixed with a solvent, a catalyst and a brominating agent are added, and the reaction is carried out to obtain ethyl 4-bromo-2,3-dihydroxybenzoate; ethyl 4-bromo-2,3-dihydroxybenzoate is mixed with a solvent, a catalyst and a base are added, and the reaction is carried out; finally, diiodomethane is added to carry out a cyclization reaction to obtain the final product. This invention uses 2,3-dihydroxybenzoic acid as a raw material, and obtains the product through esterification, regioselective bromination, and cyclization reactions. Through a specific combination of brominating agent and catalyst, highly selective bromination at the 7-position of the benzene ring is achieved, inhibiting the formation of isomers at the 5- and 6-positions.

The application relates to a preparation method of the muscle relaxant succinylcholine sodium, which comprises the following steps: adopting gamma-cyclodextrin, carrying out total halogenation, and then reacting with potassium thioacetate to prepare a key intermediate compound 6-full deoxy-6-full thioacetate-gamma-cyclodextrin, and then directly reacting with acrylic acid through one-pot synthesis to prepare succinylcholine sodium. The application has the advantages of mild reaction condition, simple post-treatment operation, good process reproducibility and high yield.