23results about How to "Simple purification process" patented technology

The invention belongs to the technical field of biomacromolecular structure analysis, and particularly relates to a compound system and application thereof in small molecular weight protein cryoelectron microscope structure analysis. In particular, the present invention provides a composite system named Trimbdy, which greatly enhances the overall rigidity and stability of the composite. By utilizing the Trimbdy system provided by the invention, the atomic resolution (less than or equal to 3) cryoelectron microscope structures of a plurality of proteins with the molecular weight of less than 50 kDa are successfully analyzed, and the potential of serving as a simple, universal and efficient tool is proved, so that the Trimbdy system has a good practical application value.

The invention discloses a benzothiazole non-condensed ring A-D-A '-D-A type organic solar cell acceptor material and a preparation method and application thereof, a benzothiazole unit is used as a central nuclear electron withdrawing unit (A-unit), and then the benzothiazole unit, the central nuclear electron withdrawing unit (A-unit), an electron donating unit (D-unit) and a terminal group are synthesized into the organic solar cell acceptor material with an A-D-A'-D-A structure. Wherein benzothiazole as an asymmetric central core structure can enhance the dipole moment of molecules, optimize the accumulation among the molecules and improve the blending property of a donor and an acceptor, and the characteristics can jointly promote charge transfer to inhibit the bimolecular recombination condition. The molecular morphology is regulated and controlled by connecting different structural groups to a top branched chain, and meanwhile, different atoms can be introduced into a benzene ring to regulate and control the chemical energy level. In addition, the polymer also has the characteristics that the aggregation behavior and the light absorption range of molecules can be adjusted by accessing different terminal groups. The benzothiazole-based non-fused ring A-D-A '-D-A type acceptor material provided by the invention is few in synthesis and preparation steps, simple in purification process and high in yield.

The application discloses an engineered exosome targeting human urokinase-type plasminogen activator receptor uPAR and a preparation method and application thereof. Firstly, a high-affinity anti-human uPAR single-domain antibody VHH is prepared; then, a human umbilical cord mesenchymal stem cell engineering cell line expressing a VHH-PTGFRN fusion protein is constructed, and the engineered exosome is prepared and purified by combining three-dimensional hypoxic culture, chemical induction and size-exclusion chromatography technology. The engineered exosome comprises four comparative samples, wherein the core sample Exo^(VHH / miR) can simultaneously display the anti-uPAR VHH on the membrane surface to realize targeted delivery and enrich miR-193b-3p in the chamber to realize gene silencing, and the synergistic effect of the two can efficiently remove the uPAR high-expression senescent cells and inhibit the senescence-associated secretory phenotype SASP. The engineered exosome has the advantages of standardized preparation process, high yield and high purity, strong targeting, excellent curative effect and good safety, and can be used for preparing a treatment preparation for anti-aging and aging-related diseases, and has important clinical application value.

The application discloses a polypeptide salt conversion device and a use method thereof, and belongs to the technical field of polypeptide preparation; the device comprises an ion exchange salt conversion kettle and a detector, and the salt conversion kettle is provided with an upper cover, a pH meter, a stirring paddle, a lower sieve plate and a feed liquid inlet and outlet; the method comprises the following steps: filling ion exchange resin in the ion exchange salt conversion kettle, loading a polypeptide liquid after the polypeptide liquid is adjusted to below or above an isoelectric point, washing the polypeptide liquid with water to remove salt, then adding an acidic or alkaline ligand solution under the continuous stirring of the stirring paddle, stirring to make the resin fully contact with the ligand and promote the polypeptide to be quickly and uniformly converted, simultaneously monitoring the pH change of the system in real time by the pH meter to accurately control the reaction end point, and finally washing and removing to obtain the product. The detector monitors the cleaning of salt ions and the elution of a sample. The application can significantly improve the salt conversion efficiency and obtain a product with low impurity residue by stirring to strengthen mass transfer and pH linkage control.

This invention discloses a red pigment-producing Streptomyces spectabilis ( Streptomyces spectabilis This invention discloses a method and application for pigment extraction from *Streptomyces spectinomyces* S-13. The method involves fermenting *Streptomyces spectinomyces* S-13 with the addition of a surfactant to extract the pigment. This method releases intracellular pigments into the fermentation broth, significantly increasing pigment yield and simplifying the extraction and purification process. The red pigment produced by *Streptomyces spectinomyces* S-13 exhibits excellent stability, antioxidant activity, and antibacterial effects, making it suitable for applications in the printing and dyeing industry. The pigment extraction method described in this invention offers advantages such as short production cycle, low cost, safety, and environmental friendliness, overcoming the limitations of traditional microbial pigment production processes, including high extraction difficulty, low yield, and limited applicability.

The present application relates to a kind of 2-oxopentanedioic acid preparation method.The invention method is as follows: 2-methylene pentanedioic acid is dissolved in a certain amount of dimethyl carbonate, a certain amount of V2O5 / K2O / zeolite composite catalyst is added, air (or oxygen) is introduced at reflux temperature for a certain time.Oxidation is carried out.After reaction, the catalyst is removed by filtration, dimethyl carbonate is removed by vacuum distillation, 2-oxopentanedioic acid crude product is obtained, the yield is 98.5%, and refined 2-oxopentanedioic acid is obtained by recrystallization with water, the purity is 99.2%.The synthesis process of the present application is simple, the yield and product purity are high, the process cost is low, and it is an environment-friendly 2-oxopentanedioic acid synthesis method.

This invention belongs to the field of wet electronic chemical purification, specifically relating to a membrane chromatography filter membrane for removing charged ions from liquids and its preparation method, including the following steps: S1, preparing casting solutions: preparing positively charged layer casting solutions, neutral layer casting solutions, and negatively charged layer casting solutions separately; S2, using a three-layer die to sequentially extrude the positively charged layer casting solutions, neutral layer casting solutions, and negatively charged layer casting solutions onto a polyester film; S3, exposing the casting solutions under set temperature, humidity, and airflow conditions to allow for initial phase separation; S4, immersing the coated film in a pure water coagulation bath to complete phase separation and solidification; S5, rinsing, drying, and then winding to obtain the membrane chromatography filter membrane. By designing a special three-layer composite structure with a "positively charged layer-neutral layer-negative layer," the filter membrane can simultaneously adsorb anions and cations in the solution during a single filtration process, overcoming the limitation of traditional single-charge membranes that can only remove one type of ion, thus improving purification efficiency.

This invention relates to the field of cleaning solutions, specifically to a highly efficient hydroxylamine-free stripping and cleaning solution. The raw materials comprise the following components by mass fraction: 10-60% water, 10-50% water-soluble organic solvent (e.g., 10%-45%), 0.1-20% amine compound (e.g., 0.5%-10%), and 1-30% hydroxylamine substitute (e.g., 2%-20%), and a small amount of corrosion inhibitor, with the sum of the mass fractions of all components being 100%. The cleaning agent provided by this method exhibits good stability, prevents corrosion of Al metal during the cleaning process, has minimal negative impact on the conductivity of subsequent applications in device interconnects, and demonstrates good safety.

This invention discloses an immune-enhancing protein, its encoding gene, and its applications. The amino acid sequence of the immune-enhancing protein described in this application is shown in SEQ ID NO.1. This protein is derived from the C-terminal portion of the flagellated protein FliC from Salmonella Typhimurium. It not only retains the inherent immune-enhancing activity of flagellated proteins but also significantly reduces their immunogenicity. When applied to porcine circovirus type 2 subunit vaccines containing aluminum adjuvants, it can effectively enhance the immunogenicity of the vaccine, induce humoral and cellular immune responses, and significantly promote antigen-specific Th1 and Th2 immune responses. This is of great significance for improving the formulation of existing aluminum adjuvanted subunit vaccines and enhancing vaccine immunization efficacy.

The invention belongs to the technical field of biology, and particularly relates to preparation and application of a swine A type FMDV VP1 antigen and single factor positive serum. The sequence of the expression gene of the swine A type FMDV VP1 antigen is as shown in SEQ ID NO. 1. Through the integral technical scheme of combining sequence-driven conservative site screening, expression and immunological verification, the key problems of antigen mismatch, difficult recombinant protein expression, poor diagnostic reagent universality and the like in the prior art are solved; and the effectiveness and generalizability of the vaccine, the diagnostic reagent and the monitoring means in practical application are obviously enhanced.

This invention belongs to the field of compound synthesis technology, and specifically relates to a method and application for the synthesis of ethyl 7-bromobenzo[D][1,3]dioxane-4-carboxylate. The synthesis method of this invention includes the following steps: 2,3-dihydroxybenzoic acid undergoes esterification to obtain ethyl 2,3-dihydroxybenzoate; ethyl 2,3-dihydroxybenzoate is mixed with a solvent, a catalyst and a brominating agent are added, and the reaction is carried out to obtain ethyl 4-bromo-2,3-dihydroxybenzoate; ethyl 4-bromo-2,3-dihydroxybenzoate is mixed with a solvent, a catalyst and a base are added, and the reaction is carried out; finally, diiodomethane is added to carry out a cyclization reaction to obtain the final product. This invention uses 2,3-dihydroxybenzoic acid as a raw material, and obtains the product through esterification, regioselective bromination, and cyclization reactions. Through a specific combination of brominating agent and catalyst, highly selective bromination at the 7-position of the benzene ring is achieved, inhibiting the formation of isomers at the 5- and 6-positions.

The application relates to a preparation method of the muscle relaxant succinylcholine sodium, which comprises the following steps: adopting gamma-cyclodextrin, carrying out total halogenation, and then reacting with potassium thioacetate to prepare a key intermediate compound 6-full deoxy-6-full thioacetate-gamma-cyclodextrin, and then directly reacting with acrylic acid through one-pot synthesis to prepare succinylcholine sodium. The application has the advantages of mild reaction condition, simple post-treatment operation, good process reproducibility and high yield.

The application discloses a preparation method of pharmaceutical-grade glacial acetic acid. It belongs to the technical field of fine chemical industry. The method uses bio-based ethanol as raw material, and performs catalytic oxidation reaction with oxygen in a reaction rectifying tower under the action of a multifunctional composite ionic liquid catalyst designed by the application. After the reaction, the crude acetic acid liquid is purified by specific molecular sieve adsorption and multi-stage low-temperature gradient crystallization in sequence. The core of the application lies in catalyst design: by utilizing the "template effect" of acetate anions and the synergistic effect of palladium-ruthenium bimetallic nanoclusters, the application realizes the ultrahigh selectivity (>99.8%) and ultralong catalytic stability of ethanol to acetic acid under mild conditions. The integrated process avoids halogen and heavy metal pollution from the source, the product purity is greater than or equal to 99.99%, the content of key impurities (formic acid, propionic acid) is reduced by 1-2 orders of magnitude compared with traditional processes, and the energy consumption is significantly reduced. Therefore, the application is an efficient and pharmaceutical glacial acetic acid preparation path.

The invention discloses a system and a method for recycling valuable metals of a lithium ion battery through cooperation of wet grinding and vacuum pyrolysis. The recycling method comprises the following steps: completely discharging a waste lithium ion battery, disassembling and separating a positive plate and a negative plate, crushing and sieving, and collecting undersize powder; mixing with a grinding medium, and carrying out ball milling by adopting a ball milling tank; the slurry is sieved after being dried, and screen underflow is collected and is electrode black powder; putting the electrode black powder into a vacuum pyrolysis reactor, vacuumizing and then pyrolyzing; and after the pyrolysis is finished, cooling to room temperature under vacuum or inert gas protection to obtain a valuable metal solid product. The method is carried out at a relatively low temperature, metal oxidation can be inhibited, generation of harmful gas is reduced, separation and pyrolysis with higher adaptability to raw materials are realized, and directional recovery is realized.

The application discloses a recombinant plasmid for expressing salt-tolerant protease, a yeast engineering bacterium, a fermentation medium thereof and application. Key elements for heterologous expression of the salt-tolerant protease are confirmed, and the recombinant plasmid for expressing the salt-tolerant protease and the yeast engineering bacterium are constructed. The long-term salt-tolerance level and SDS tolerance of the salt-tolerant protease expressed by the yeast engineering bacterium are significantly improved, and the salt-tolerant protease can effectively and significantly degrade soybean protein isolate under high-temperature and high-salt conditions, so that the salt-tolerant protease has important application value for efficient catalysis of protein substrates in traditional high-salt fermentation industry. The application also realizes efficient secretion of the salt-tolerant protease by optimizing the concentration of enzyme cofactors. Furthermore, electrophoretically pure grade recombinant salt-tolerant protease is obtained through one-step separation and purification, and the purification process is simple and efficient.

The application discloses a synthesis method of xylulose-5-phosphate. The method takes a commercially available compound 1 as a starting material, and obtains the target product xylulose-5-phosphate through seven independent step-by-step reactions. The intermediates are compound 2 to compound 7 in turn. The synthesis method provided by the application is a step-by-step reaction, each reaction is an independent unit, the process parameters are accurately controllable and suitable for industrial operation errors, the side reactions are few, and the product selectivity is high. The method innovatively uses tetrabenzyl pyrophosphate as a phosphorylation reagent, replaces expensive reagents, and reduces the synthesis cost. The reaction condition of the method is mild, the operation is convenient, the crude compound 2 and the crude compound 4 can be directly used in the next step reaction, the post-treatment process is simple, and the route is simple and easy to be industrialized. The synthesis method of xylulose-5-phosphate has stable yield and accurate product structure, is suitable for large-scale industrialized preparation of xylulose-5-phosphate, and can effectively meet the market demand in the field of biological medicines.

The present application belongs to the technical field of caprolactone purification, and particularly relates to a caprolactone purification method and a finished caprolactone purified by the method. The method comprises the following steps: mixing a caprolactone-containing material to be purified with a first extractant to perform first extraction to obtain a first extraction material; removing oxides from the first extraction material to obtain a primary purified material; performing first concentration on the primary purified material to obtain a concentrated material; mixing the concentrated material with a second extractant to perform second extraction to obtain a second extraction material; mixing the second extraction material with a third extractant to perform third extraction to obtain a third extraction material; and performing second concentration on the third extraction material to obtain a finished caprolactone. The caprolactone purified by the purification method has the advantages of simple process and low equipment investment. The finished caprolactone has high yield, and the purity of the finished caprolactone is greatly improved compared with the existing process.

This disclosure provides a method for preparing a class of camptothecin-based conjugates. The novel method for preparing camptothecin-based conjugates disclosed herein has one or more advantages selected from the group consisting of: high yield; simple purification process with low impurities; suitable for industrial production; and high stability.

The invention relates to the technical field of medicines, in particular to a synthesis method of an antibiotic Clovibactin. According to the method disclosed by the invention, efficient and controllable preparation of the antibiotic Clovibactin can be realized, the problems of production stability and large-scale production are solved, the consistency and repeatability of product batches are ensured through determined chemical reaction steps and conditions, and a foundation is laid for large-scale standardized production.

This invention provides 13 High-abundance CO was prepared by CuO catalytic oxidation. 13 This invention relates to a CO2 apparatus and method, belonging to the field of stable isotope-labeled compound preparation technology. The apparatus described in this invention is an integrated unit comprising a feed gas supply unit, a fixed-bed reaction unit, a cryogenic collection unit, a partial pressure purification unit, and a tail gas treatment unit connected in series. The fixed-bed reaction unit includes a fixed-bed reactor, a heater, and a temperature sensor. This invention utilizes high-temperature calcined modified CuO catalyst particles, combined with a composite bed structure design of "quartz wool-quartz sand-catalyst-quartz sand," significantly improving conversion efficiency and selectivity; optimizing the purification process, achieving product quality that meets high-end application standards; featuring a novel apparatus design with greatly improved sealing and stability; simple and controllable operation, facilitating industrial scale-up; and being green, safe, and environmentally friendly, aligning with industrial development trends.

The present application relates to the field of bioengineering, and particularly relates to a mutant of mu-conotoxin and a preparation method and an enzyme composition thereof.The present application provides a mutant of mu-conotoxin, which has an amino acid sequence as shown in SEQ ID NO:1, or an amino acid sequence obtained by substituting, deleting or adding one or more amino acids in the amino acid sequence, and an amino acid sequence which is functionally identical or similar to the amino acid sequence, or an amino acid sequence which is at least 80% identical to the amino acid sequence.The technical route and process provided by the present application greatly improve the production efficiency and quality of mu-conotoxin, are suitable for industrial large-scale production, and have a wide application prospect.

This invention belongs to the field of lithium-ion battery material preparation technology, and discloses a one-step method for preparing lithium bis(fluorosulfonyl)imide using ionic liquid catalysis and hydrogen fluoride. This method solves the problems of low catalytic efficiency, insufficient product purity, difficult separation, and high environmental impact in existing preparation routes. Using bis(fluorosulfonyl)imide and lithium fluoride as raw materials, anhydrous hydrogen fluoride as the fluorinating agent, and an imidazole-based ionic liquid as the catalyst, under an inert atmosphere, the reaction system is prepared, followed by an integrated fluorination-lithiation reaction and post-treatment purification steps to obtain high-purity lithium bis(fluorosulfonyl)imide. This invention achieves efficient reaction under mild conditions through the synergistic effect of a specific ionic liquid and hydrogen fluoride gas, solving the problems of harsh reaction conditions, low yield, and insufficient purity in existing processes. The product yield is ≥92%, and the purity is ≥99%. The process is simple, safe, and environmentally friendly, suitable for large-scale industrial production, and can be widely used in the preparation of lithium-ion battery electrolytes.