1333 results about "Biological engineering" patented technology

The invention is applicable to the field of biological engineering and provides a method for detecting mononucleotide polymorphism. The method comprises the following steps: sequencing fragments obtained by high throughput sequencing technology are compared on a referenced genome sequence; the likelihood ratio of various genotypes of the corresponding sites on the genome to be tested is obtained according to the sequencing mass fraction of each basic group in the genome to be tested and obtained by sequencing; the posterior probability of each genotype of each site on the referenced genome is calculated according to the likelihood ratio and the prior probability preset for each genotype, and the genotype which has the highest posterior probability is determined as the most likely right genotype of the corresponding sites on the genome to be tested to obtain the consistent sequence of the genome to be tested; and the sites of the genome to be tested, which are inconsistent with the sequence of the referenced genome in the consistent sequence are detected to obtain the polymorphism sites of the genome to be tested. The embodiment of the invention can achieve a more accurate test result as the influence of the prior probability on mononucleotide polymorphism test result is considered in advance.

The invention relates to a visual false body image processing device and the method, belonging to the field of biological engineering. In the invention, an image collecting module is a CMOS digital image sensor which converts the obtained image information into digital mode; a wireless transmission module is responsible for the communication of the image collecting module and the image processing module, then transmits the image information collected by the image collecting module to the image processing module; the image processing module has an image information precorrection to the obtained image to improve the image quality, having image information simplifying, strengthening or coding treatment to transform the obtained image information into the pulse encouraging mode accepted by the visual system to provide for an internal stimulation module, and providing the function of self regulation for users. The method is showed as follows: firstly executing image pretreatment, then through an image complexity judgment, the image is divided into three kinds and the corresponding treatment procedure is adopted. The invention resolves the prior disadvantage to be applied in all types of visual false body.

The invention relates to screening and application of saccharomyces cerevisiae suitable for the brewing of a series of kirschwasser, pertaining to the technical field of biological engineering. SC203 strain of the saccharomyces cerevisiae has been preserved in Center of General Microbiology of China Committee for Culture Collection of Microorganisms, with a registration number of CGMCC No. 2786. The strain is characterized by rapid growth, early fermentation, short fermentation cycle, strong tolerance against low temperature, acid, alcohol, sulfur dioxide and the like and is applicable to the brewing of such a series of kirschwasser as kirschwasser, ice kirschwasser, kirschwasser liqueur, kirschwasser ratafee and the like. In addition, the strain can also be used for the brewing of grape wine and other common ratafee and icewine.

The invention provides a method for producing fermented tea by biological engineering method. The invention absorbs the traditional tea fermentation process, and fully employs the achievements of modern fermentation biological engineering research. Multiple fungus, microzyme and probiotic bacteria, which satisfy the relevant industry requirements and meet with the microbe categories for natural fermentation tea, compose the microbe combination; after water-removing and rolling, the fresh tea leaves enter biofermentation process without external water source so that the quality and security of products can be guaranteed. The invention has few process links and simple operation, greatly reduces the fermentation period and improves the production efficiency, implements the technical scale; the fermented tea has the same health-care function as the tea preserved for many years, and has good smell, lasting appeal, color, soup color and excellent taste.

The invention relates to the technical field of biological engineering, in particular to a method for preparing antihypertensive peptide by utilizing combined enzyme enzymolysis silkworm chrysalis protein. The method comprises the following steps: dissolving degreased dry silkworm chrysalis powder or silkworm chrysalis protein powder in water in the mass ratio of 1: 7 to 8, adjusting the pH value of the dissolved solution to be 7.5 to 8.5, and then adding combined protease to carry out enzymolysis reaction, wherein the content of alkaline protease is 0.3 to 0.5 percent of the degreased dry silkworm chrysalis powder or the silkworm chrysalis protein powder, the content of papain is 0.05 to 0.1 percent, the content of neutral protease is 0.05 to 0.1 percent, the enzymolysis reaction temperature is 55 to 65 DEG C, and the enzymolysis reaction time is 180 to 240 minutes. The prepared antihypertensive peptide has higher restriction effect on the ACE activity, antihypertensive function on hypertensive patients, does not influence normotensive patients, plays the better health care effect and is safe, has no poison or side effect and is low in cost.

The invention belongs to the technical field of biological engineering and in particular is a heat-induced fusion expression recombinant plasmid of truncated GST protein and a process for preparation. The invention selects utility truncated GST188 protein which is composed of 188aa as a carrier, a pXXGST-1 recombinant plasmid which expresses short peptide fusion protein in an escherichia coli heat-induced expression system and a heat-induced type pXXGST-2 recombinant plasmid which is used to express comparison to realize the aim of synthesizing short peptide creatures, which is especially provided for scanning, drawing, positioning antigen linear epitopes and identifying epitope motif. Experiments of the invention prove the adaptability and the applicability of the GST188 core protein which is used as the short peptide creatures expression vector, in antigen epitope scanning identification, and in particular when anti- recombinant target protein antiserum is used to indentify the epitope motif.

The invention discloses a magnetic ultrahigh cross-linking adsorbent resin and a preparation method thereof, belonging to the field of magnetic resin. The ultrahigh cross linking adsorbent resin provided by the invention is formed by a framework and magnetic particles wrapped in the framework, wherein the resin framework has the following basic structural formula that the saturation magnetization intensity of the resin is 0.1-10e mu/g; and the average particle size of the resin is 60-300 mu m. The resin provided by the invention is synthesized by adopting a suspension polymerization method and a reaction system comprises an oil phase, magnetic particles and a water phase, wherein the magnetic particles are wrapped in a double layer mode through a coupling agent and a dispersing agent, thereby enhancing the acid-resistant property and oil-phase dispersing property of the magnetic particles; chloromethyl styrene is adopted as a monomer to be synthesized into magnetic chlorine balls by adopting a one-step method; and then, a cross-linking reaction is carried out on the magnetic chlorine balls under the condition of nitrogen protection and in the presence of a buffer agent, thereby preparing the magnetic ultrahigh cross-linking adsorbent resin. The magnetic ultrahigh cross-linking adsorbent resin prepared by the invention can be applied to the elimination of organic pollutants in water, in particular to micro-pollution organic matters, and has wide application prospects in the fields of biological engineering, chemical analysis and the like.

The invention discloses a method for preparing xylose and arabinose by hydrolyzing lignocelluloses in the technical field of biological engineering. In the method, aqueous oxalate solution is used for carrying out catalytic hydrolysis on crushed lignocelluloses; lignocelluloses hydrolysate solution and xylose resides are obtained by solid-liquid separation after the reaction; the hydrolysate solution is neutralized to obtain oxalate sediment and obtain solid oxalate by solid-liquid separation again; the oxalate reacts with sulfate in an acidizing tank for regeneration to obtain the aqueous oxalate solution; the oxalate solution is used repeatedly to catalyze next batch of lignocelluloses raw material. The invention uses oxalic acid as organic acid catalyst and has the advantages of high conversion rate, strong selectivity, less side reaction, being capable of recycling, and the like, the oxalic acid can replace regular inorganic acid catalyst, and the invention solves the problem thatthe traditional technology has equipment erosion, evaporating equipment scaling, serious pollution and the like and can be used for hydrolyzing the hemicellulose in lignocelluloses raw material and used for preparing the xylose and the arabinose.

The invention provides a preparation method of 3'-sialic acid lactose, belonging to the technical field of biological engineering. The preparation method comprises the following steps of: taking colibacillus CCTCC (colibacillus China center for type culture collection) NO: M208088 as a starting strain, adding a fermentation medium into a fermentation tank, fermenting for 2-6h, then adding a fed batch culture medium, and adding lactose in a flowing way at the mid-later period of the fermentation; and centrifuging the fermentation liquid to obtain liquid supernatant 1 and a thallus, diluting the centrifuged thallus by adding water and splitting in a heating way, recentrifuging to obtain liquid supernatant 2, merging the liquid supernatant 1 and the liquid supernatant 2, and absorbing, desorbing and vacuum drying by ion exchange resin to obtain the 3'-sialic acid lactose. A mass of the 3'-sialic acid lactose can be prepared by the CCTCC NO: M208088. The 3'-sialic acid lactose can change the current situation that the sialyloligosaccharide component is less in exogenous adding substances in conventional baby milk, and can provide the guarantee to add more efficient sialic acid trophic factors into the baby milk.

The invention relates to a novel method for extracting anthocyanin in bilberry by double water phases, and belongs to the technical field of biological engineering. The method is characterized by comprising the following steps: adding inorganic salt solution and hydrophilic low-molecular organic substance into bilberry fruit residue, stirring the mixture evenly, keeping the mixture standing, layering the mixture, extracting the anthocyanin to an upper phase, and distributing the residue at the lower phase or between the two phases so as to achieve the aim of extracting the anthocyanin from the bilberry fruit residue. The inorganic salt is one or a combination of sodium chloride, ammonium sulphate, sodium sulfate, sodium carbonate, potassium carbonate, potassium phosphate, monopotassium phosphate and dipotassium hydrogen phosphate, preferably ammonium sulphate; and the hydrophilic low-molecular organic substance is one or a combination of methanol, ethanol, normal propyl alcohol, isopropyl alcohol, normal butanol, isobutyl alcohol, glycol and acetone, preferably ethanol. The method has the advantages that a novel method for extracting the anthocyanin is developed, the method is easy to industrialize, the utilization ratio of the bilberry is improved, and the production cost of the anthocyanin is lowered.

A method for preparing solid natto bacillus microecological agent by solid fermenting is disclosed, which belongs to the biological engineering technique field. The strain is separated from natto as natto bacillus which is named as Bacillus natto S703 and the preservation number of the strain is CCTCC M207147. The sieving method comprises the following steps: utilizing the character that the natto bacillus produces natto kinase to do dilute flat separation for natto leach, seeding the obtained single colony in the primary sieving culture medium of the flat which contains fiber, selecting single colony to purify and obtain the fitful natto bacillus according to the size of transparent coil diameter and colony diameter. The strain is inoculated in the solid ferment culture medium which comprises bran, soya bean waste, corn starch, dipotassium hydrogen phosphate and magnesium sulfate, which can produce the natto bacillus microecological agent by solid fermenting, drying and grinding. The prepared natto bacillus microecological agent can be chicken feed, which is provided with obvious modification for daily chicken weighing, feed coefficient and survival rate.

The invention discloses a production method of water-soluble functional monascus powder, which belongs to the technical fields of biological engineering and health product preparation. In the invention, the water-soluble functional monascus powder containing a Monacolin K is prepared the following steps of: taking a high-yielding Monacolin K purple monascus without a notalin as a starting bacterium, carrying out solid state fermentation of corns, then extracting, condensing, adjusting the pH, adding cyclodextrine and embedding a water-soluble corn hydrolysate and spray drying; or the corn is fermented in a liquid state and then a fermentation liquid is manufactured into the water-soluble functional monascus powder containing the Monacolin K through removing a thalli by filtering, adjusting the pH, adding the cyclodextrine and embedding the water-soluble corn hydrolysate and spray drying; and detail researches are carried out on the technical conditions of fermenting, extracting, embedding and spray drying, thus obtaining the optimized technical parameters; the product has excellent water-solubility; the Monacolin K content in the monascus powder product can achieve 20g/kg; the Monacolin K proportion of an open ring structure can achieve more than 90 percent. The water solution which is prepared by the water-soluble functional monascus powder product of 20 percent can maintain clear and transparent under the room temperature, does not contain deposition and has better stability.

The invention discloses fruit vinegar tea drink. The fruit vinegar tea drink is prepared from the following raw materials in part by weight: 50 to 70 parts of apple juice, 5 to 8 parts of white granulated sugar, 1 to 2 parts of honey and 1 to 1.2 parts of black tea powder. A preparation method comprises the following steps of: adding concentrated apple juice into fruit vinegar tea serving as a main raw material, wherein the fruit vinegar tea is prepared from fresh apple juice, black tea powder, white granulated sugar and honey, which serve as main raw materials, through biological engineering submerged secondary fermentation; and mixing, performing superhigh temperature instant sterilization, and performing encapsulation at medium temperature to obtain health-care drink with various nutrients. The fruit vinegar tea drink has high content of nutritional ingredients, good mouthfeel and can meet requirements of people on health-care drink. The fruit vinegar tea has effects of eliminating fatigue, reducing morbidity of liver diseases, resisting ageing, softening blood vessels, reducing blood fat, reducing cholesterol and preventing and treating obesity, and has high sterilization ability; apple vinegar and black tea are integrated into a whole; requirements of people on health promotion are met due to the combination of the apple vinegar and the black tea; and the apple vinegar and the black tea can be mutually promoted and supplemented.

The invention relates to a method for designing a high-density outer-layer artificial retina system, belonging to the field of the biological engineering. The method comprises the following steps: a charge-coupled device (CCD) image sensor is used for acquiring extraocular video images; the extraocular video images are pre-processed; the region of interest (ROI) in the extraocular video images is extracted by using a saliency map and a visual attention saliency map; the high-precision extraocular video images are effectively mapped and then the low-precision extraocular video images are effectively mapped; after the ROI in the extraocular video images is encoded and modulated, the data is wirelessly transmitted by using one pair of coupling coils and the energy is wirelessly transmitted by using another pair of coupling coils so as to effectively solve the conflict between the high-transmission data volume and the high-transmission energy efficiency of a wireless transmission unit; on the receiving end, after the modulated data is demodulated and decoded, a micro-stimulator adopts the driving unit multiplexing method to effectively reduce the area and adopts the wide-swing cascode circuit design and the charge balance mechanism to improve the biological adaptation and the security; and the RF energy is subjected to the current rectifying and the voltage stabilizing to supply energy to all implanted equipment.

Screening and application of yeast with high ethanol yield and low fusel oil yield in Chinese Maotai-flavor liquor production belong to the technical field of biological engineering. The strain is any one strain selected from pichia anomala CGMCC4740, schizosaccharomyces pombe CGMCC4744, zygosaccharomyces bailii CGMCC4745, trichosporon asahii CGMCC4746, saccharomyces cerevisiae CGMCC4747, kazachstania exigua CGMCC4748, pichia fermentans CGMCC4750. The strain is separated from various Daqu, fermented grains, brewing raw materials and brewing environment of several Chinese famous distilleries; the screening method comprises the following steps: diluting distiller yeast samples, fermented grains, brewing raw materials or brewing environment substances, coating on a TTC primary screening plate, selecting a colony with an obvious red color, inoculating the colony in a shake flask for culture, performing secondary screening, determining the contents of ethanol and fusel oil of the fermentation broth by a distillation specific gravity method and HS-SPME-GC-MS respectively, screening the strain with high ethanol concentration and low fusel oil concentration. The strain is applicable to food industry and brewed wine industry.

The invention relates to the field of biological engineering. The culture medium formula of selenium-enriched edible fungi comprises the following components in percentage by weight: 0.4 to 0.6 percent of bean straw chopper, 0.15 to 0.25 percent of leaf powder, 0.05 to 0.1 percent of magnesium sulfate, 0.2 to 0.4 percent of corn flour, 0.2 to 0.5 percent of selenium-enriched rice bran, and 0.1 to0.3 percent of ginkgo leaf powder. The selenium-enriched edible fungi producing method comprises the following steps: preparing a culture medium; regulating; sterilizing; inoculating and culturing; and accelerating mushrooms, wherein, during liquid strain culture, a sodium selenite component is added in a culture solution; and a strain is cultured in the culture solution for culturing the strain.The cultured selenium-enriched edible fungi have high content of selenium, can be used for effectively improving the selenium level in blood of a human body and improving the disease resistance of the human body, and have excellent disease treatment and body-building efficacies.

The invention is applicable to the field of biological engineering, and provides an environmental microorganism detection method and a system thereof. The method comprises the following steps: sequencing the DNA extracted from environment samples by the high throughput sequencing technology to obtain a DNA tag sequence; eliminating carrier pollution existing in DNA tag sequence; comparing the DNAtag sequence with the known sequence in the known database, and determining the category to which the DNA tag sequence belongs according to the comparison result. The embodiment of the invention can detect which microorganism species or which class of microorganism species possibly exists in environment samples.

The present invention relates to the embankment protection in water conservancy and hydroelectric power system, especially inner slope in water reservoir. The plant configuration and ecological engineering technology of the present invention is superior to traditional concrete, laid stone wall, anchor pile and other traditional rigid means, which are lack of ecological effect of purifying water and lasting effect, and superior to available biological engineering means with non ideal effect. The present invention is one ecological engineering technology with vetiver as main body and other plant variety, and has superior ecological effect, lasting slope protection effect, low cost and simple construction process.

The invention discloses a method for separating microorganisms from grease, which relates to a method for directly extracting grease from microorganism fermenting liquor for producing oil and co-producing microbial dreg and belongs to the technical field of downstream post treatment of biological engineering. The invention adopts the following technical scheme: mixing the microorganism fermenting liquor for producing oil and organic solvent according to certain ratio; stirring and leaching for a certain time; performing the solid-liquid separating operation; removing solvent from an organic solvent phase to obtain grease; and drying a solid to obtain the microbial dreg. The invention has the advantages of simple process, mild operating conditions, high extraction rate and yield of grease, good quality of microbial dreg, low energy consumption and favorability for scale industrial production.

The invention relates to a strain capable of producing L-arginine and a method for producing the L-arginine by the strain and belongs to the technical field of biological engineering. For the strain, Brevibacterium flavum ATCC 14067 is used as a starting strain; nitrosoguanidine is adopted to carry out mutagenesis step by step; mutant strains with histidine and succinic acid auxotrophic strains are screened out so as to cut off a competitive metabolic pathway; and mutant strains (His-, Suc-, D-Argr, SMCr) with resistances of arginine structural analogs and cysteine structural analogs are screened out. The strain is named as Brevibacterium flavum HX1009, is preserved in the China general microbiological culture collection center, has the preservation number of CGMCC No.4464 and has the genetic characters of histidine auxotroph His-, succinic acid auxotroph Suc-, D-arginine resistance D-Argr and S-methyl cysteine resistance SMCr for improving the yield of the L-arginine. Under the optimized condition, the L-arginine is produced by fermentation on a fermentation tank with the volume of 5L to 5M3 and the arginine production level achieves 50 to 70g/L.

The invention discloses Pichia pastoris strain with alpha-1, 6-mannosyl transferase absent and the establishing method, which belongs to the biological engineering field. The collection number of the Pichia pastoris strain with alpha-1, 6-mannosyl transferase absent is CGMCC No.1853. The invention has the advantages that the Pichia pastoris strain with alpha-1, 6-mannosyl transferase absent built by the invention can prevent the generation of excessive manna saccharify, when expressing extrinsic glucoprotein, reduce the immunogenicity of the pressed protein, and therefore, the invention has important application value in the biomedical field, and other fields; simultaneously, the strain can also be applied to the further gene knockout or the metabolic engineering reconstruction. The invention also builds a method for knocking out the alpha-1, 6-mannosyl transferase gene through secondary homologous recombination, mutant strain can be relatively easily obtained through the method, therefore the sieving workload is greatly reduced, and the success ratio is improved.

The invention relates to the field of biological engineering and in particular relates to the field of culture of edible fungi. The production method of the calcium-enriched edible fungi comprises the following steps: preparing raw materials of a culture medium, adding an ionized calcium solvent into the selected raw materials of the culture medium, and then smashing the raw materials of the culture medium, which contain the ionized calcium solvent, thereby forming the raw materials of the calcium-enriched culture medium; adding water into the raw materials of the calcium-enriched culture medium for stirring, and uniformly stirring for preparing a mixture of the culture medium; loading the well stirred mixture of the culture medium into a culture bottle for culturing the edible fungi; and performing sterilization treatment on the culture bottle in a sterilization device. A formula of the culture medium of the calcium-enriched edible fungi comprises the raw materials of the culture medium, and the raw materials of the culture medium comprise 1%-3% by weight of the ionized calcium solvent. By utilizing the technical scheme, the produced edible fungi has the advantages of short fruiting time and high calcium content in the edible fungi.