97 results about "Biological engineering" patented technology

A federated distributed computational system enables secure, privacy-preserving biological data analysis and engineering through interconnected nodes coordinated in a distributed graph architecture. A federation manager allocates resources, manages data flow and lineage, establishes privacy boundaries, and maintains cross-institutional knowledge relationships. Each node contains a processing unit for biological data analysis, privacy preservation protocols for secure multi-party computation, a knowledge graph structure with supporting data stores, and encrypted network connections. The federation manager enforces all computation and data exchange through secure channels while maintaining privacy, security, and contractual boundaries. This architecture enables research institutions to collaborate on complex biological analyses without compromising sensitive data, facilitating breakthrough discoveries through shared computational resources while maintaining strict data privacy and security controls.

The invention provides a fluorescent protein selection marker staygold-ynaMr gene and application thereof, and belongs to the technical field of biological engineering. The method comprises the following steps: replacing codons of L-isoleucine in nucleotide sequences of a fluorescent protein gene staygold and a gene ynaM by using a rare codon ATA to respectively obtain the fluorescent protein gene staygold after codon replacement and the gene ynaMr after codon replacement, and connecting the two segments of genes after codon replacement by using a flexible protein peptide, and the fluorescent protein selection marker staygold-ynaMr gene is obtained. Experiments prove that the gene can obviously improve the screening efficiency of L-isoleucine high-yield strains.

The invention provides a genetically engineered bacterium and a construction method of the genetically engineered bacterium, and the genetically engineered bacterium is characterized in that a green fluorescent protein sfGFP is used for mediating protein glutaminase, PG (protein glutaminase), PGF (protein glutaminase), PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF and PGF; eC 3.5. 1.44) is efficiently secreted and expressed in engineering bacteria, and belongs to the technical field of biological engineering. The method comprises the following steps: an obtained sfGFP gene is derived from an NCBI database (GenBank numbering: CP035486.1), an obtained PG zymogen gene PP (Propeptide-Protein glutaminase) is derived from a Chryseobacterium prion prgA gene (GenBank numbering: AB046594.1) in the NCBI database, a recombinant plasmid pHT01 / sfGFP-PP is introduced into bacillus subtilis WB800N to obtain an engineering bacterium, extracellular secretion expression of PP is realized, the sfGFP-PP is activated by trypsin to obtain PG, and the PG is subjected to enzyme activation to obtain the recombinant bacillus subtilis. The expression quantity and the secretion efficiency of the PG in the bacillus subtilis are greatly improved. The recombinant PG produced by the method can generate 23.5 U / mL enzymatic activity under activation of trypsin, can be used for improving protein functional characteristics, and has relatively high industrial production and application values.

The invention provides a humanized monoclonal antibody for detecting a human-synuclein oligomer and application of the humanized monoclonal antibody, and belongs to the technical field of biological engineering. The light chain CDR sequence of the binding part comprises at least one of LCDR1, LCDR2 and LCDR3, and the amino acid sequence of the LCDR1 is as shown in SEQ ID NO: 1; the amino acid sequence of the LCDR2 is as shown in SEQ ID NO: 2, and the amino acid sequence of the LCDR3 is as shown in SEQ ID NO: 3. The monoclonal antibody is used as a raw material, and a unique repeated epitope double-antibody sandwich immunodetection method is adopted, so that the human a-Syn oligomer prepared in vitro or in a sample can be detected with high specificity, high sensitivity and high accuracy.

The invention provides application of acidophilous glycosyltransferase in salidroside production, and belongs to the technical field of biological engineering. The problem of producing salidroside under the acidic condition is solved. Comprising an application of acidophilous glycosyl transferase with an amino acid sequence as shown in SEQ ID NO.1 in salidroside production under an acidic condition and an acidophilous escherichia coli engineering strain for producing salidroside. The escherichia coli engineering strain overexpresses a mutant 3-deoxy-D-arabinoheptulose-7-phosphate (DAHP) synthetase gene aroGfbr, a cyclohexadiene dehydrogenase gene tyrC, a glucose phosphate mutant enzyme gene pgm and a UDP-glucose pyrophosphorylase galU, overexpresses a phenylpyruvate decarboxylase gene ARO10 derived from saccharomyces cerevisiae, and can be used for producing a mutant 3-deoxy-D-arabinoheptulose-7-phosphate mutant enzyme. The kit comprises an ethanol dehydrogenase gene ADH6 and a glycosyl transferase gene LrUGT85AF8. The method is mainly used for producing salidroside under an acidic condition.

The invention discloses a recombinant escherichia coli engineering strain for producing levodopa and application, and belongs to the technical field of genetic engineering and bioengineering. Escherichia coli WSH-Z06 is used as a starting strain, a levodopa synthesis route is constructed, and supply of cofactors is enhanced by introducing glucose dehydrogenase BmgdH; and the catalytic efficiency of the HpaB is greatly improved by modifying the entrance of the HpaB substrate tunnel. In addition, by optimizing the fermentation pH and induction time, the accumulation amount of the levodopa in the fermentation liquor reaches 60.7 g / L, the production intensity reaches 1.26 g / L / h, a foundation is laid for industrial production of the levodopa, and potential value and significance are achieved for development of synthetic biology.

The invention discloses a schizochytrium limacinum strain for efficiently converting kitchen waste to produce single-cell protein and application of the schizochytrium limacinum strain, and belongs to the technical field of biological engineering. The preservation number of the schizochytrium sp LOX-2 is CGMCC (China General Microbiological Culture Collection Center) No. 41623. The invention also discloses a method for producing single-cell protein by efficiently converting kitchen waste. The method comprises the following steps: fermenting food waste to produce volatile fatty acid; and culturing schizochytrium limacinum by using the strain as a carbon source to obtain single-cell protein. According to the recombinant strain spLOX-2, the utilization of lactic acid is improved, the biomass and the protein yield of the recombinant strain spLOX-2 are respectively improved by 14.19% and 12% compared with those of a wild strain, and the grease content is obviously improved by 107.1%. According to the invention, a new way is provided for a sustainable biological economic mode, wastes are converted into valuable biological chemicals, and a solid foundation is laid for expanding the scale of the process and the economic feasibility of the process in industrial application.

The application discloses a fusion protein NGF2 with improved in-vivo half-life and application thereof, and belongs to the technical field of medical biological engineering, and comprises ABD and FGF2, and the gene and protein sequence thereof are respectively composed of the sequences shown in SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:6 and SEQ ID NO:7, and the complete gene and protein sequence thereof is shown in SEQ ID NO:4 and SEQ ID NO:8. The fusion protein NGF2 can keep activity for more than 7 days in an environment of 37 DEG C, and the half-life of the fusion protein in solution is prolonged. In addition, the ABD is combined with HAS to form a complex, and the total molecular weight reaches 90kDa, which is higher than the cut-off molecular weight of glomerular filtration, so that the in-vivo half-life of the fusion protein is effectively prolonged.

The utility model relates to an automatic sampling system of a culture tank. Through accurate control of the control system, automation of the sampling process is achieved, manual intervention is not needed, the sampling efficiency is greatly improved, and the labor intensity is reduced. By utilizing the high-precision sensing device and the control system, the sampling time and the sampling amount can be accurately controlled, the accuracy and the consistency of each sampling are ensured, and the reliability of experimental data is improved. Due to the application of the sterile air driving mechanism and the one-way valve, external pollutants are effectively prevented from being introduced in the sampling process, the purity of a culture environment is guaranteed, and the pollution risk is reduced. By monitoring parameters such as pressure in the culture tank in real time and feeding data back to the control system, the system can intelligently regulate and control the sampling process, and smooth sampling operation is ensured. Through the automatic, high-precision and pollution-free design, the sampling efficiency and accuracy in the cell culture and fermentation process in the field of bioengineering are remarkably improved.

The invention belongs to the technical field of biological engineering, and discloses a schizochytrium limacinum engineering strain co-expressing a PDC-ACC gene, a construction method and application, and the genetic engineering strain is obtained by taking schizochytrium limacinum as an original strain and expressing a pyruvate decarboxylase compound gene PDC and an acetyl-coenzyme A carboxylase gene ACC in yarrowia lipolytica. The PDC and ACC genes in the schizochytrium limacinum genetic engineering strain disclosed by the invention are cloned to yarrowia lipolytica, namely Po1f. The yield of traditional fermented grease is 43.90 g / L. The engineering strain performs overexpression on PDC and ACC genes, so that the grease content of the schizochytrium limacinum engineering strain is increased by 53.13% and reaches 67.22 g / L, and the DHA content is increased by 36.97% and is increased from 40.71% to 55.76%.

The invention provides an anti-human phosphorylated tau217 rabbit monoclonal antibody as well as a preparation method and application thereof, and belongs to the technical field of biological detection and biological engineering. The rabbit monoclonal antibody for resisting the human phosphorylated tau217 comprises a light chain variable region and a heavy chain variable region, and the monoclonal antibody comprises HZK33-B0010, HZK33-B0011 and HZK33-B0029. The invention develops three rabbit monoclonal antibodies for identifying p-tau217, the rabbit monoclonal antibodies do not have cross reaction with non-phosphorylated tau protein or phosphorylated tau protein at other sites, the rabbit monoclonal antibodies can be combined with p-tau217 with high specificity, and a related body fluid marker detection kit with higher specificity is developed on the basis of the rabbit monoclonal antibodies.

The invention discloses a low-temperature-resistant pseudomonas with a biocontrol effect and application thereof, relates to the field of microorganisms, and aims to solve the problem that the pathogen antagonism capability is reduced due to the fact that the activity of the existing biocontrol strain is inhibited in a low-temperature environment. The pseudomonas is pseudomonas koreana B12, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation date is February 17, 2025, and the preservation number is CGMCC No.33538. The pseudomonas koreana B12 can be applied to the field of biological engineering. The low-temperature-resistant pseudomonas disclosed by the invention is used for inhibiting phytopathogens at a low temperature of 5-15 DEG C and is used for decomposing organic phosphorus, glucan, starch and cellulose.

The invention relates to the technical field of biological engineering, and particularly discloses an integrated cell treatment super-clean bench, which comprises a super-clean bench body with a fan system, and a pipetting mechanism, an uncovering mechanism, a centrifugal mechanism and a pipe cap opener which are arranged in the super-clean bench body, the super clean bench further comprises a first feeding and discharging mechanism and a second feeding and discharging mechanism, the first feeding and discharging mechanism is used for feeding / discharging culture dishes into / out of the super clean bench body through the feeding and discharging channel, and the second feeding and discharging mechanism is used for feeding / discharging test tubes into / out of the super clean bench body through the feeding and discharging channel. According to the scheme, the problem that a culture dish storage area and a test tube storage area are arranged in the super clean bench and occupy a large space in integrated equipment is solved.

The invention discloses a method for preparing the ginsenoside preparation by biological engineering technology, wherein the ginsenoside preparation comprises at least one of the saponin-enriched preparation and the saponin-enriched syrup; the compound bio-enzyme preparation prepared by the combined bacteria preparation of yeast and lactic acid bacteria provided by the invention is rich in amino acid residues, amides, coenzymes and active groups such as lactic acid and linoleic acid; and the ginsenoside preparations containing ginsenosides can be prepared by adding monosaccharides or polysaccharides to the compound bio-enzyme preparations, providing new directions and ideas for obtaining ginsenosides and their products.

The invention belongs to the technical field of biological engineering, and particularly relates to a construction method and application of yeast engineering bacteria for producing 1-aminocyclopropane-1-carboxylic acid (ACC). Pyruvate carboxylase gene RoPYC, yeast SAM enzyme gene Sam2 and ACS synthase gene GmACS are overexpressed in saccharomyces cerevisiae, so that the 1-aminocyclopropane-1-carboxylic acid (ACC) is obtained; a path for biosynthesizing 1-aminocyclopropane-1-carboxylic acid (ACC) by taking glucose as a substrate is designed, a saccharomyces cerevisiae engineering bacterium for producing ACC is constructed, the ACC can be produced by large-scale fermentation, and the highest yield can reach 50.56 g / L. The method has the advantages of low environmental influence, easiness in large-scale production expansion, high production efficiency and the like, has a good economic prospect, and lays a foundation for artificial efficient biosynthesis of ACC.

The invention discloses an AMP sulfating enzyme mutant for producing adenosine phosphoryl sulfuric acid and application, and belongs to the technical field of biological engineering. According to the invention, AMP is used as a raw material, and AMP sulfating enzymes BtaAPSST M2 and PcAPSK are used for biosynthesis of PAPS. Under the catalysis of 10 g / L of BtaAPSST M2 wet thallus and 20 g / L of PcAPSK wet thallus, the conversion rate of PAPS is 70.59%. Compared with the existing ATP sulfating enzyme capable of catalyzing ATP to generate APS, the ATP sulfating enzyme disclosed by the invention is lower in cost and equivalent in conversion rate. Wherein the PAPS yield of the BtaAPSST M2 is two times that of a wild enzyme, and the industrial process of producing the PAPS by a microbial synthesis method is accelerated.

The utility model relates to biological engineering equipment technical field, concretely relates to a biochemical incubator, including base, is equipped with the box on the base, is fixed in the box incubator, is pasted with the conduction sheet on the incubator, and the semiconductor refrigeration heating module is connected with the conduction sheet, and one side of the box is equipped with air filtration system, and is communicated with the incubator through the circulation pipe, and the ultrasonic humidifier is equipped below the semiconductor refrigeration heating module, and is communicated with the incubator through the pipeline, and the incubator is equipped with the sealed door, and there is the gap between the incubator and the box and forms the heat insulation layer, and the controller is connected with the temperature and humidity sensor group, gas sensor group electric signal, can automatically regulate and control each module according to real -time data.

The invention discloses application of a corn Zm00001d028750 gene in regulation and control of grain traits, and relates to the technical field of bioengineering, a nucleotide sequence of a coding region of the Zm00001d028750 gene is as shown in SEQ ID NO.1, and an amino acid sequence of a protein coded by the Zm00001d028750 gene is as shown in SEQ ID NO.2. The invention further discloses application of the corn Zm00001d028750 gene in regulation and control of grain traits and application of the corn Zm00001d028750 gene in regulation and control of grain traits. According to the application of the corn Zm00001d028750 gene in regulation and control of grain traits, the hundred-grain weight of corn is an important factor influencing the yield of corn, and along with the increase of the hundred-grain weight of corn, the higher the seed yield is, the higher the yield is; lysine is an essential amino acid for human beings and monogastric animals, and the eating and feeding values of corn can be remarkably improved by increasing the content of lysine and total amino acids.

The invention discloses issatchenkia orientalis capable of efficiently synthesizing L-malic acid and application of the issatchenkia orientalis, and belongs to the technical field of biological engineering. In an existing gene edited yeast strain in a laboratory, endogenous lactic dehydrogenase IoLDH2 and IoLDH3 are over-expressed, endogenous IoLDH1 is knocked out, and lactic dehydrogenase EcD-LDH from escherichia coli and lactic dehydrogenase NmD-LDH from neisseria meningitidis are over-expressed. After fermentation for 72 h in a 5 L fermentation tank, the accumulation amount of L-malic acid reaches 296.27 g / L, the yield is 0.97 g / g, the production intensity is 4.11 g / L / h, and the by-product lactic acid is remarkably reduced from 23.56 g / L to 6.7 g / L and is reduced by 71.56%.

This invention discloses a caltrop-shaped sand barrier and its bioengineering method for sand fixation, belonging to the field of ecological protection and desertification control technology. The caltrop-shaped sand barrier includes several main arms and at least 1-3 radial arms. The ends of the arms can be sharpened and inserted into the sand layer 10-20 cm or directly spread on the sand surface. The spacing between groups is varied according to terrain differences, with a density of 0.5-0.8 m on windward slopes and dune tops, and 0.8-1.5 m on flat sandy areas. Native sand-loving shrubs and grasses are mixed and sown in the gaps between the arms and within the coverage area. The seeds can be pelleted or coated with water-retaining agents and biological agents. Direct sowing or simultaneous sowing in functional planting bags is used. No fertilization or watering is required throughout the process, combined with 3 years of sand-fixing and grass cultivation management. This invention enables the synergistic operation of physical and biological sand fixation. The sand barriers have strong wind resistance and high efficiency in preventing wind and blocking sand, increasing the survival rate of plants by 20% to 50% and reducing treatment costs by 30% to 40%. Stable and long-lasting biological sand barriers can be formed in 2 to 3 years. It is suitable for the treatment of various desertification areas such as mobile sand dunes, deserts, severely degraded grasslands, and abandoned wastelands.

The invention discloses photoresponse hydrogel as well as a preparation method and application thereof, and belongs to the technical field of intelligent materials. The preparation method specifically comprises the following steps: (1) synthesizing a compound 1; (2) synthesizing a compound 2; (3) dissolving acryloyl chloride in dichloromethane, dropwise adding into a dichloromethane mixed solution containing the compound 2 and triethylamine, stirring, washing, concentrating under reduced pressure, and recrystallizing; and (4) dissolving N, N-dimethylacrylamide, a phenylboronic acid monomer, diol, MEH and N, N '-methylene bisacrylamide in water, filling nitrogen, adding azodiisobutyronitrile, gelatinizing, and dialyzing, so as to obtain the hydrogel. The hydrogel based on visible light driven deformation is designed and prepared, controllable change of shape, volume or motion can be achieved through illumination, and the core application of the hydrogel comprises soft robot application, medical and biological engineering application, environment and energy application and other innovative application.

The invention belongs to the technical field of molecular biology, and particularly discloses a method for improving citrus canker resistance by using a CsENPP1 gene, the coding protein of the CsENPP1 gene is citrus nucleotide pyrophosphatase / phosphodiesterase 1, the coding sequence of the CsENPP1 is a nucleotide sequence shown as SEQ ID No.1, and the citrus canker resistance is improved based on overexpression of the CsENPP1 gene in citrus cells. The citrus nucleotide pyrophosphatase / phosphodiesterase 1 coding gene is integrated into citrus through an expression vector, the canker attack degree of the obtained transgenic material can be reduced to 48.9% of that of the existing citrus to the maximum extent, the canker attack degree can be remarkably reduced, the scab area is reduced, and the yield of the citrus is improved. The technology provided by the invention is a bioengineering technology which has potential to improve the citrus canker resistance, and has great value for citrus canker resistance molecular breeding.

The present disclosure provides a ribose-modified cap analog and a use thereof, and belongs to the technical field of chemical and biological engineering. The ribose-modified cap analog has a structure of formula (I). The ribose-modified cap analog described herein can improve the stability of mRNA and / or the translation efficiency of mRNA.

The invention relates to the technical field of biological engineering, in particular to application of a gene ZmNPF6.8 in regulation and control of corn nitrogen absorption and utilization efficiency and single-plant yield of corn. The gene ZmNPF6.8 has the following characteristics: 1) a coding region nucleotide sequence as shown in SEQ ID NO: 3; and 2) a nucleotide sequence which has more than 90% of homology with the nucleotide sequence as shown in SEQ ID NO: 3 and forms protein with the same function as the nucleotide sequence as the nucleotide sequence as shown in SEQ ID NO: 3. According to the invention, the expression quantity of the gene ZmNPF6.8 can be down-regulated by inserting a Mutuator transposon into an amino acid coding region of the gene, and meanwhile, the nitrogen absorption and utilization efficiency and the yield per plant of corn are remarkably reduced. Researches show that the nitrogen absorption and utilization efficiency of the corn and the change of the yield of a single plant of the corn can be regulated and controlled by changing the expression quantity of the gene ZmNPF6.8, and the gene is of great significance to the high-efficiency and high-yield breeding work of the corn in the future.

The application discloses a tissue culture regeneration system and culture method of Hunan millet, and relates to the field of biological engineering. The regeneration system is to take the Hunan millet seeds with removed hulls as explants, and utilizes a culture medium composed of 1 / 2MS basic culture medium, 0.6-0.99 mg / L 2,4-D, 30 g / L sucrose, 7 g / L agar and 0.1% PPM, with pH being 5.8, to make the explants form complete Hunan millet plants. The regeneration system is used for tissue culture of the Hunan millet, effectively avoiding the problems of long culture period, high requirement for personnel and high culture cost caused by diversification of culture medium configuration in large-scale factory production. The application provides a simpler and more efficient method for large-scale factory production, research on gene function and molecular breeding and the like.

This invention belongs to the field of biological engineering technology, specifically relating to an MXene-based photothermal coupling composite film for bone repair and its preparation method; comprising MXene, type I collagen, and gelatin solution, obtained by sequential bridging and cross-linking with FeCl3 solution; wherein the mass ratio of MXene to type I collagen is (3-9):(1-7), and gelatin accounts for 3.6%-10.4% of the final composite material mass; the preparation method includes: ultrasonically dispersing MXene and mixing it with gelatin solution, then compounding it with type I collagen solution, vacuum filtering and drying to form a matrix film, and finally cross-linking it by impregnation with FeCl3 solution; this film has mechanical properties matching natural bone tissue, good biocompatibility, and high-efficiency photothermal conversion capability under near-infrared light irradiation, and is suitable for bone defect repair and photothermal synergistic therapy.

The application discloses an acetylacetone cleavage enzyme mutant and a method for generating 2-acetylcyclohexanone through an extracellular enzyme reaction, and belongs to the technical field of biological engineering. In order to solve the technical problem of how to improve the yield of 2-acetylcyclohexanone generated through an extracellular enzyme reaction, the problem of limited Dke1 substrate types in a whole-cell synthesis system is solved, and the toxicity of 2-acetylcyclohexanone to cells is inhibited. The application provides an acetylacetone cleavage enzyme mutant, wherein the mutant is obtained by mutating the amino acid at the 105th position of the acetylacetone cleavage enzyme with the amino acid sequence shown in SEQ ID NO. 3 to alanine from glycine, and the yield of 2-acetylcyclohexanone generated through an enzyme reaction of the mutant in vitro reaches 14.22 mg / L.

The invention discloses a waste battery metal recovery method based on microorganisms, and belongs to the technical field of biological engineering. The method comprises the following steps: (1) producing citric acid by using microorganisms, and forming a composite leaching system by using the citric acid, gluconic acid generated by catalyzing glucose by engineering pichia pastoris with glucose oxidase displayed on the surface and a reducing agent; battery recycling residues (namely black powder) are added into the composite leaching system for biological leaching; and (2) specifically adsorbing target metal ions from the leachate by using an engineered pichia pastoris strain of which the surface displays the metal binding peptide. According to the recycling method disclosed by the invention, on the basis of mild process conditions, no need of strong acid / strong alkali and remarkable reduction of environmental pollution, efficient recycling of the metal ions is also realized, and an efficient and sustainable solution is provided for recycling of the waste batteries.

The invention discloses a method for constructing a nasal mucosa epithelium single-layer model by using a single-cell digested organoid, and belongs to the technical field of bioengineering. The method comprises the following steps: firstly, carrying out digestion treatment, red blood cell lysis treatment, inoculated culture and passage on nasal mucosa tissues to obtain nasal mucosa organoid, then adding recombinase for dissociation to obtain dissociated epithelial cells, finally, planting the dissociated epithelial cells in a culture container subjected to coating treatment for incubation and adherence, and continuing to culture to obtain the nasal mucosa organoid. And preparing the nasal mucosa epithelium single-layer model. The multi-lineage differentiation potential and individual specificity of the organoid-derived cells are reserved, the cell adherence, passage and cryopreservation recovery stability is remarkably improved, the culture period is shortened, reagent expenditure and time cost are saved, and the defects that a traditional cell line is poor in physiological correlation, difficult in primary and gas-liquid interface culture acquisition, long in period and low in cost are overcome. And the three-dimensional organ-like chamber structure is not beneficial to top surface observation and solute transport research, so that the three-dimensional organ-like chamber structure is more suitable for scientific experiments.