84 results about "Biological engineering" patented technology

The invention provides a fluorescent protein selection marker staygold-ynaMr gene and application thereof, and belongs to the technical field of biological engineering. The method comprises the following steps: replacing codons of L-isoleucine in nucleotide sequences of a fluorescent protein gene staygold and a gene ynaM by using a rare codon ATA to respectively obtain the fluorescent protein gene staygold after codon replacement and the gene ynaMr after codon replacement, and connecting the two segments of genes after codon replacement by using a flexible protein peptide, and the fluorescent protein selection marker staygold-ynaMr gene is obtained. Experiments prove that the gene can obviously improve the screening efficiency of L-isoleucine high-yield strains.

The invention provides application of acidophilous glycosyltransferase in salidroside production, and belongs to the technical field of biological engineering. The problem of producing salidroside under the acidic condition is solved. Comprising an application of acidophilous glycosyl transferase with an amino acid sequence as shown in SEQ ID NO.1 in salidroside production under an acidic condition and an acidophilous escherichia coli engineering strain for producing salidroside. The escherichia coli engineering strain overexpresses a mutant 3-deoxy-D-arabinoheptulose-7-phosphate (DAHP) synthetase gene aroGfbr, a cyclohexadiene dehydrogenase gene tyrC, a glucose phosphate mutant enzyme gene pgm and a UDP-glucose pyrophosphorylase galU, overexpresses a phenylpyruvate decarboxylase gene ARO10 derived from saccharomyces cerevisiae, and can be used for producing a mutant 3-deoxy-D-arabinoheptulose-7-phosphate mutant enzyme. The kit comprises an ethanol dehydrogenase gene ADH6 and a glycosyl transferase gene LrUGT85AF8. The method is mainly used for producing salidroside under an acidic condition.

The invention discloses a recombinant escherichia coli engineering strain for producing levodopa and application, and belongs to the technical field of genetic engineering and bioengineering. Escherichia coli WSH-Z06 is used as a starting strain, a levodopa synthesis route is constructed, and supply of cofactors is enhanced by introducing glucose dehydrogenase BmgdH; and the catalytic efficiency of the HpaB is greatly improved by modifying the entrance of the HpaB substrate tunnel. In addition, by optimizing the fermentation pH and induction time, the accumulation amount of the levodopa in the fermentation liquor reaches 60.7 g / L, the production intensity reaches 1.26 g / L / h, a foundation is laid for industrial production of the levodopa, and potential value and significance are achieved for development of synthetic biology.

The application discloses a fusion protein NGF2 with improved in-vivo half-life and application thereof, and belongs to the technical field of medical biological engineering, and comprises ABD and FGF2, and the gene and protein sequence thereof are respectively composed of the sequences shown in SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:6 and SEQ ID NO:7, and the complete gene and protein sequence thereof is shown in SEQ ID NO:4 and SEQ ID NO:8. The fusion protein NGF2 can keep activity for more than 7 days in an environment of 37 DEG C, and the half-life of the fusion protein in solution is prolonged. In addition, the ABD is combined with HAS to form a complex, and the total molecular weight reaches 90kDa, which is higher than the cut-off molecular weight of glomerular filtration, so that the in-vivo half-life of the fusion protein is effectively prolonged.

The utility model relates to an automatic sampling system of a culture tank. Through accurate control of the control system, automation of the sampling process is achieved, manual intervention is not needed, the sampling efficiency is greatly improved, and the labor intensity is reduced. By utilizing the high-precision sensing device and the control system, the sampling time and the sampling amount can be accurately controlled, the accuracy and the consistency of each sampling are ensured, and the reliability of experimental data is improved. Due to the application of the sterile air driving mechanism and the one-way valve, external pollutants are effectively prevented from being introduced in the sampling process, the purity of a culture environment is guaranteed, and the pollution risk is reduced. By monitoring parameters such as pressure in the culture tank in real time and feeding data back to the control system, the system can intelligently regulate and control the sampling process, and smooth sampling operation is ensured. Through the automatic, high-precision and pollution-free design, the sampling efficiency and accuracy in the cell culture and fermentation process in the field of bioengineering are remarkably improved.

The invention provides an anti-human phosphorylated tau217 rabbit monoclonal antibody as well as a preparation method and application thereof, and belongs to the technical field of biological detection and biological engineering. The rabbit monoclonal antibody for resisting the human phosphorylated tau217 comprises a light chain variable region and a heavy chain variable region, and the monoclonal antibody comprises HZK33-B0010, HZK33-B0011 and HZK33-B0029. The invention develops three rabbit monoclonal antibodies for identifying p-tau217, the rabbit monoclonal antibodies do not have cross reaction with non-phosphorylated tau protein or phosphorylated tau protein at other sites, the rabbit monoclonal antibodies can be combined with p-tau217 with high specificity, and a related body fluid marker detection kit with higher specificity is developed on the basis of the rabbit monoclonal antibodies.

The invention discloses a method for preparing the ginsenoside preparation by biological engineering technology, wherein the ginsenoside preparation comprises at least one of the saponin-enriched preparation and the saponin-enriched syrup; the compound bio-enzyme preparation prepared by the combined bacteria preparation of yeast and lactic acid bacteria provided by the invention is rich in amino acid residues, amides, coenzymes and active groups such as lactic acid and linoleic acid; and the ginsenoside preparations containing ginsenosides can be prepared by adding monosaccharides or polysaccharides to the compound bio-enzyme preparations, providing new directions and ideas for obtaining ginsenosides and their products.

The utility model relates to biological engineering equipment technical field, concretely relates to a biochemical incubator, including base, is equipped with the box on the base, is fixed in the box incubator, is pasted with the conduction sheet on the incubator, and the semiconductor refrigeration heating module is connected with the conduction sheet, and one side of the box is equipped with air filtration system, and is communicated with the incubator through the circulation pipe, and the ultrasonic humidifier is equipped below the semiconductor refrigeration heating module, and is communicated with the incubator through the pipeline, and the incubator is equipped with the sealed door, and there is the gap between the incubator and the box and forms the heat insulation layer, and the controller is connected with the temperature and humidity sensor group, gas sensor group electric signal, can automatically regulate and control each module according to real -time data.

The invention discloses application of a corn Zm00001d028750 gene in regulation and control of grain traits, and relates to the technical field of bioengineering, a nucleotide sequence of a coding region of the Zm00001d028750 gene is as shown in SEQ ID NO.1, and an amino acid sequence of a protein coded by the Zm00001d028750 gene is as shown in SEQ ID NO.2. The invention further discloses application of the corn Zm00001d028750 gene in regulation and control of grain traits and application of the corn Zm00001d028750 gene in regulation and control of grain traits. According to the application of the corn Zm00001d028750 gene in regulation and control of grain traits, the hundred-grain weight of corn is an important factor influencing the yield of corn, and along with the increase of the hundred-grain weight of corn, the higher the seed yield is, the higher the yield is; lysine is an essential amino acid for human beings and monogastric animals, and the eating and feeding values of corn can be remarkably improved by increasing the content of lysine and total amino acids.

The invention discloses issatchenkia orientalis capable of efficiently synthesizing L-malic acid and application of the issatchenkia orientalis, and belongs to the technical field of biological engineering. In an existing gene edited yeast strain in a laboratory, endogenous lactic dehydrogenase IoLDH2 and IoLDH3 are over-expressed, endogenous IoLDH1 is knocked out, and lactic dehydrogenase EcD-LDH from escherichia coli and lactic dehydrogenase NmD-LDH from neisseria meningitidis are over-expressed. After fermentation for 72 h in a 5 L fermentation tank, the accumulation amount of L-malic acid reaches 296.27 g / L, the yield is 0.97 g / g, the production intensity is 4.11 g / L / h, and the by-product lactic acid is remarkably reduced from 23.56 g / L to 6.7 g / L and is reduced by 71.56%.

This invention discloses a caltrop-shaped sand barrier and its bioengineering method for sand fixation, belonging to the field of ecological protection and desertification control technology. The caltrop-shaped sand barrier includes several main arms and at least 1-3 radial arms. The ends of the arms can be sharpened and inserted into the sand layer 10-20 cm or directly spread on the sand surface. The spacing between groups is varied according to terrain differences, with a density of 0.5-0.8 m on windward slopes and dune tops, and 0.8-1.5 m on flat sandy areas. Native sand-loving shrubs and grasses are mixed and sown in the gaps between the arms and within the coverage area. The seeds can be pelleted or coated with water-retaining agents and biological agents. Direct sowing or simultaneous sowing in functional planting bags is used. No fertilization or watering is required throughout the process, combined with 3 years of sand-fixing and grass cultivation management. This invention enables the synergistic operation of physical and biological sand fixation. The sand barriers have strong wind resistance and high efficiency in preventing wind and blocking sand, increasing the survival rate of plants by 20% to 50% and reducing treatment costs by 30% to 40%. Stable and long-lasting biological sand barriers can be formed in 2 to 3 years. It is suitable for the treatment of various desertification areas such as mobile sand dunes, deserts, severely degraded grasslands, and abandoned wastelands.

The invention discloses photoresponse hydrogel as well as a preparation method and application thereof, and belongs to the technical field of intelligent materials. The preparation method specifically comprises the following steps: (1) synthesizing a compound 1; (2) synthesizing a compound 2; (3) dissolving acryloyl chloride in dichloromethane, dropwise adding into a dichloromethane mixed solution containing the compound 2 and triethylamine, stirring, washing, concentrating under reduced pressure, and recrystallizing; and (4) dissolving N, N-dimethylacrylamide, a phenylboronic acid monomer, diol, MEH and N, N '-methylene bisacrylamide in water, filling nitrogen, adding azodiisobutyronitrile, gelatinizing, and dialyzing, so as to obtain the hydrogel. The hydrogel based on visible light driven deformation is designed and prepared, controllable change of shape, volume or motion can be achieved through illumination, and the core application of the hydrogel comprises soft robot application, medical and biological engineering application, environment and energy application and other innovative application.

The invention belongs to the technical field of molecular biology, and particularly discloses a method for improving citrus canker resistance by using a CsENPP1 gene, the coding protein of the CsENPP1 gene is citrus nucleotide pyrophosphatase / phosphodiesterase 1, the coding sequence of the CsENPP1 is a nucleotide sequence shown as SEQ ID No.1, and the citrus canker resistance is improved based on overexpression of the CsENPP1 gene in citrus cells. The citrus nucleotide pyrophosphatase / phosphodiesterase 1 coding gene is integrated into citrus through an expression vector, the canker attack degree of the obtained transgenic material can be reduced to 48.9% of that of the existing citrus to the maximum extent, the canker attack degree can be remarkably reduced, the scab area is reduced, and the yield of the citrus is improved. The technology provided by the invention is a bioengineering technology which has potential to improve the citrus canker resistance, and has great value for citrus canker resistance molecular breeding.

The present disclosure provides a ribose-modified cap analog and a use thereof, and belongs to the technical field of chemical and biological engineering. The ribose-modified cap analog has a structure of formula (I). The ribose-modified cap analog described herein can improve the stability of mRNA and / or the translation efficiency of mRNA.

The invention relates to the technical field of biological engineering, in particular to application of a gene ZmNPF6.8 in regulation and control of corn nitrogen absorption and utilization efficiency and single-plant yield of corn. The gene ZmNPF6.8 has the following characteristics: 1) a coding region nucleotide sequence as shown in SEQ ID NO: 3; and 2) a nucleotide sequence which has more than 90% of homology with the nucleotide sequence as shown in SEQ ID NO: 3 and forms protein with the same function as the nucleotide sequence as the nucleotide sequence as shown in SEQ ID NO: 3. According to the invention, the expression quantity of the gene ZmNPF6.8 can be down-regulated by inserting a Mutuator transposon into an amino acid coding region of the gene, and meanwhile, the nitrogen absorption and utilization efficiency and the yield per plant of corn are remarkably reduced. Researches show that the nitrogen absorption and utilization efficiency of the corn and the change of the yield of a single plant of the corn can be regulated and controlled by changing the expression quantity of the gene ZmNPF6.8, and the gene is of great significance to the high-efficiency and high-yield breeding work of the corn in the future.

The application discloses a tissue culture regeneration system and culture method of Hunan millet, and relates to the field of biological engineering. The regeneration system is to take the Hunan millet seeds with removed hulls as explants, and utilizes a culture medium composed of 1 / 2MS basic culture medium, 0.6-0.99 mg / L 2,4-D, 30 g / L sucrose, 7 g / L agar and 0.1% PPM, with pH being 5.8, to make the explants form complete Hunan millet plants. The regeneration system is used for tissue culture of the Hunan millet, effectively avoiding the problems of long culture period, high requirement for personnel and high culture cost caused by diversification of culture medium configuration in large-scale factory production. The application provides a simpler and more efficient method for large-scale factory production, research on gene function and molecular breeding and the like.

This invention belongs to the field of biological engineering technology, specifically relating to an MXene-based photothermal coupling composite film for bone repair and its preparation method; comprising MXene, type I collagen, and gelatin solution, obtained by sequential bridging and cross-linking with FeCl3 solution; wherein the mass ratio of MXene to type I collagen is (3-9):(1-7), and gelatin accounts for 3.6%-10.4% of the final composite material mass; the preparation method includes: ultrasonically dispersing MXene and mixing it with gelatin solution, then compounding it with type I collagen solution, vacuum filtering and drying to form a matrix film, and finally cross-linking it by impregnation with FeCl3 solution; this film has mechanical properties matching natural bone tissue, good biocompatibility, and high-efficiency photothermal conversion capability under near-infrared light irradiation, and is suitable for bone defect repair and photothermal synergistic therapy.

The application discloses an acetylacetone cleavage enzyme mutant and a method for generating 2-acetylcyclohexanone through an extracellular enzyme reaction, and belongs to the technical field of biological engineering. In order to solve the technical problem of how to improve the yield of 2-acetylcyclohexanone generated through an extracellular enzyme reaction, the problem of limited Dke1 substrate types in a whole-cell synthesis system is solved, and the toxicity of 2-acetylcyclohexanone to cells is inhibited. The application provides an acetylacetone cleavage enzyme mutant, wherein the mutant is obtained by mutating the amino acid at the 105th position of the acetylacetone cleavage enzyme with the amino acid sequence shown in SEQ ID NO. 3 to alanine from glycine, and the yield of 2-acetylcyclohexanone generated through an enzyme reaction of the mutant in vitro reaches 14.22 mg / L.

The invention discloses a waste battery metal recovery method based on microorganisms, and belongs to the technical field of biological engineering. The method comprises the following steps: (1) producing citric acid by using microorganisms, and forming a composite leaching system by using the citric acid, gluconic acid generated by catalyzing glucose by engineering pichia pastoris with glucose oxidase displayed on the surface and a reducing agent; battery recycling residues (namely black powder) are added into the composite leaching system for biological leaching; and (2) specifically adsorbing target metal ions from the leachate by using an engineered pichia pastoris strain of which the surface displays the metal binding peptide. According to the recycling method disclosed by the invention, on the basis of mild process conditions, no need of strong acid / strong alkali and remarkable reduction of environmental pollution, efficient recycling of the metal ions is also realized, and an efficient and sustainable solution is provided for recycling of the waste batteries.

The invention discloses a method for constructing a nasal mucosa epithelium single-layer model by using a single-cell digested organoid, and belongs to the technical field of bioengineering. The method comprises the following steps: firstly, carrying out digestion treatment, red blood cell lysis treatment, inoculated culture and passage on nasal mucosa tissues to obtain nasal mucosa organoid, then adding recombinase for dissociation to obtain dissociated epithelial cells, finally, planting the dissociated epithelial cells in a culture container subjected to coating treatment for incubation and adherence, and continuing to culture to obtain the nasal mucosa organoid. And preparing the nasal mucosa epithelium single-layer model. The multi-lineage differentiation potential and individual specificity of the organoid-derived cells are reserved, the cell adherence, passage and cryopreservation recovery stability is remarkably improved, the culture period is shortened, reagent expenditure and time cost are saved, and the defects that a traditional cell line is poor in physiological correlation, difficult in primary and gas-liquid interface culture acquisition, long in period and low in cost are overcome. And the three-dimensional organ-like chamber structure is not beneficial to top surface observation and solute transport research, so that the three-dimensional organ-like chamber structure is more suitable for scientific experiments.

The invention discloses a method for synthesizing levosalbutamol and a precursor thereof through three-enzyme cascade catalysis, and belongs to the technical field of biological engineering. The invention designs a new route for efficiently biologically synthesizing the levosalbutamol and the precursor thereof by taking cheap 5-[2-[(1, 1-dimethylethyl) amino] acetyl]-2-hydroxybenzaldehyde as a substrate through three-enzyme cascade catalysis of benzyl alcohol dehydrogenase, carbonyl reductase and glucose dehydrogenase. When the substrate concentration is 100 mM, the yield of the levosalbutamol reaches 98%, and the enantiomeric excess (ee) is greater than 99%. In addition, according to the route, 5-bromoacetyl-2-hydroxybenzaldehyde can be used as a substrate, and the levosalbutamol precursor can be efficiently synthesized through three-enzyme cascade catalysis. Compared with the existing method for mainly producing the levosalbutamol and the precursor thereof, the method disclosed by the invention has the advantages that the production cost is obviously reduced, the synthesis efficiency and the chiral purity of the product are improved, and the method has a wide application prospect.

The invention relates to glycosyltransferase, a nucleic acid fragment, a recombinant expression vector, a host cell and application of the glycosyltransferase. The amino acid sequence of the glycosyl transferase is as shown in SEQ ID NO: 1. According to the application, an enzyme for converting 3-oxo-ionol glycosyl into 3-oxo-ionol glucoside is found from tobacco, namely, a novel glycosyl transferase is provided, and the function of the enzyme in biosynthesis of related glycosyl transferase genes is cloned and verified for the first time; in vitro, the recombinant protein can be used for efficiently converting a 3-oxo-ionol substrate into corresponding glycoside. The invention further provides a recombinant plasmid containing the glycosyl transferase gene, glucoside can be massively synthesized through a bioengineering method, meanwhile, the invention further provides application of the recombinant plasmid capable of massively synthesizing 3-oxo-ionol glucoside, more basic elements are provided for synthetic biology of natural products, and the recombinant plasmid can be used for preparing a large amount of 3-oxo-ionol glucoside. In addition, guidance and basis are provided for rational design of the enzymes, and the method has good industrialization prospects.

The application provides a rat TEX264 recombinant protein, a recombinant vector, a polyclonal antibody and application, and relates to the technical field of biological engineering.The amino acid sequence of the rat TEX264 recombinant protein is shown as SEQ ID NO.2.The application provides a preparation method of an anti-rat TEX264 polyclonal antibody, and a polyclonal antibody against rat TEX264 is prepared; the antibody can specifically recognize endogenous TEX264 protein of a rat, and is used for experiments such as ELISA, Western Blot, immunofluorescence analysis and the like.The application provides a material basis for studying the function of TEX264.The antibody of the application can detect TEX264 protein of human and mouse tissues in addition to recognizing and detecting rat TEX264, compared with a related antibody on the market which can only detect TEX264 protein of mouse and / or human tissue samples; the antibody can simultaneously detect TEX264 protein of rat, mouse and human tissue samples, and is suitable for various different detection methods such as Western Blot, immunohistochemistry and immunofluorescence, and has advantages in economic benefits.

The invention discloses a genetically engineered bacterium for producing cyanidin-3-O-rutinoside as well as a construction method and application of the genetically engineered bacterium, and relates to the technical field of biological engineering. The invention provides an application of escherichia coli in production of cyanidin 3-O rutinoside. The modified recombinant escherichia coli can be used for efficient biosynthesis of cyanidin 3-O-rutinoside through whole-cell catalysis. Compared with plant extraction, the method has the advantages that the production conditions are mild, the product yield is increased, the specific synthesis of the product is realized, and an efficient and environment-friendly method with a wide development prospect is provided for the production and application of the cyanidin-3-O-rutinoside.

The invention provides engineering probiotics EcN-Vh as well as a construction method and application thereof, and belongs to the technical field of biological medicines. According to the present invention, the engineering probiotic capable of expressing the BTApep-TAT is constructed through the bioengineering technology, the BTApep-TAT is expressed through the plasmid integration mode, the release of the peptide is achieved by using the arabinose-induced cracking system, and the engineering probiotic is used for the in-vivo sustainable production of the BTApep-TAT. The engineering probiotic EcN-Vh disclosed by the invention can continuously generate an inhibitory peptide BTApep-TAT of a targeted BORIS (Bovine Biosensor), and is used for treating the colorectal cancer. According to the probiotics, by inhibiting ADP-ribosylation and Wnt / beta-catenin signal channels of BORIS, the occurrence rate and progress of tumors are remarkably reduced, and a novel, safe and efficient probiotic delivery system is provided for treatment of colorectal cancer.

The invention discloses a genetically engineered bacterium for catalyzing synthesis of ornamental blue from an ornamental blue derivative as well as a construction method and application of the genetically engineered bacterium, and relates to the technical field of bioengineering. According to the invention, on the basis of a gene editing technology, CobB, NOX, katE, NAMPT, nadD, rbsk, prs and ppk genes are integrated onto an escherichia coli genome; a genetically engineered bacterium for catalyzing synthesis of the observation blue derivative (N-acetylobservation blue or N, N '-diacetyl-observation blue) is constructed, synthesis of NAD + can be enhanced, cyclic regeneration of NAD + can be realized, H2O2 which is generated in the synthesis process of NAD + and has a toxic effect on enzyme is eliminated, supply of cofactors PRPP and ATP is enhanced, deacetylase is activated, and the observation blue derivative (N-acetylobservation blue or N, N'-diacetyl-observation blue) is enabled to be converted into the observation blue derivative (N-acetylobservation blue or N, N '-diacetyl-observation blue) into the observation blue derivative (N-acetylobservation blue or N, N'-diacetyl-observation blue). N, N '-diacetyl-observation blue) is used for catalytic synthesis of observation blue.

The present disclosure belongs to the technical field of biological engineering, and in particular to a temperature-sensitive aroma-producing Saccharomyces cerevisiae and use thereof in preparation of flavor enhancers. The temperature-sensitive aroma-producing Saccharomyces cerevisiae is preserved in China Center for Type Culture Collection on May 31, 2024, with the preservation number of CCTCC NO: M20241116. The present disclosure adopts a mixed fermentation method of temperature-sensitive aroma-producing Saccharomyces cerevisiae and Corynebacterium glutamicum (the preservation number is CCTCC NO: M 2024431) to obtain a fermentation broth containing high concentrations of glutamic acid and nucleotides. Simultaneously, the fermentation broth also contains a variety of functional nutrients, such as other amino acids, organic acids and β-glucan, enabling higher functional nutritional value.

The invention discloses application of an inhibitor acting on NOS1 related ceRNA in relieving sheep endometritis caused by LPS and promoting embryo implantation, and relates to the field of bioengineering. The inhibitor is si-NOS1 (SEQ ID No.1) and / or si-TCONS00000014 (SEQ ID No.2), and by regulating and controlling a NOS1 / bta-miR-370 / TCONS00000014 signal axis, the endometrial oxidative stress excessive activation induced by LPS (lipopolysaccharide) is improved, the autophagy and apoptosis of cells are relieved, and the expression of a key implantation gene is recovered. Experiments prove that the inhibitor can significantly improve the sheep embryo implantation rate and reduce the embryo loss probability, provides a new target and strategy for preventing and treating LPS-induced sheep endometritis and embryo implantation disorder, and has important significance for improving the sheep breeding efficiency.

The invention relates to the technical field of biological engineering, and discloses a method for producing agricultural tryptophan through fermentation and application, and the method comprises the following steps: after thalli are accumulated, adding L-serine in a pulse manner, switching to double-carbon-source material supplementation of glucose and glycerol, and starting energy metabolism uncoupling control at the same time; according to the energy metabolism decoupling control, a decoupling agent is fed, a real-time respiration quotient is used as a feedback signal, and a closed-loop system is used for automatically adjusting the flow acceleration rate, so that the respiration quotient is maintained in a target range. According to the invention, intracellular energy charge is reduced through uncoupling so as to relieve glycolysis inhibition and strengthen carbon flux, double carbon sources are utilized to maintain redox balance, and pulse feeding is matched to eliminate terminal synthesis bottleneck. According to the method, the metabolic competition problem of cell growth and product synthesis is effectively solved, the conversion rate and fermentation yield of tryptophan are effectively improved, and the obtained product is suitable for preparing an agricultural fertilizer synergist or a feed additive.