Patents
Literature
52 results about "Biological engineering" patented technology
Efficacy Topic
Property
Owner
Technical Advancement
Application Domain
Technology Topic
Technology Field Word
Patent Country/Region
Patent Type
Patent Status
Application Year
Inventor
Biological engineering, or bioengineering/bio-engineering, is the application of principles of biology and the tools of engineering to create usable, tangible, economically viable products. Biological engineering employs knowledge and expertise from a number of pure and applied sciences, such as mass and heat transfer, kinetics, biocatalysts, biomechanics, bioinformatics, separation and purification processes, bioreactor design, surface science, fluid mechanics, thermodynamics, and polymer science. It is used in the design of medical devices, diagnostic equipment, biocompatible materials, renewable bioenergy, ecological engineering, agricultural engineering, and other areas that improve the living standards of societies. Examples of bioengineering research include bacteria engineered to produce chemicals, new medical imaging technology, portable and rapid disease diagnostic devices, prosthetics, biopharmaceuticals, and tissue-engineered organs. Bioengineering overlaps substantially with biotechnology and the biomedical sciences in a way analogous to how various other forms of engineering and technology relate to various other sciences (for example, aerospace engineering and other space technology to kinetics and astrophysics).
A fusion protein ngf2 with improved half-life in vivo and its use
The application discloses a fusion protein NGF2 with improved in-vivo half-life and application thereof, and belongs to the technical field of medical biological engineering, and comprises ABD and FGF2, and the gene and protein sequence thereof are respectively composed of the sequences shown in SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:6 and SEQ ID NO:7, and the complete gene and protein sequence thereof is shown in SEQ ID NO:4 and SEQ ID NO:8. The fusion protein NGF2 can keep activity for more than 7 days in an environment of 37 DEG C, and the half-life of the fusion protein in solution is prolonged. In addition, the ABD is combined with HAS to form a complex, and the total molecular weight reaches 90kDa, which is higher than the cut-off molecular weight of glomerular filtration, so that the in-vivo half-life of the fusion protein is effectively prolonged.
Owner:TRIUMPH WORLD GROUP CO LTD
Anti-human phosphorylated tau217 rabbit monoclonal antibody as well as preparation method and application thereof
ActiveCN121717902ANervous disorderImmunoglobulins against animals/humansAntiendomysial antibodiesHeavy chain
The invention provides an anti-human phosphorylated tau217 rabbit monoclonal antibody as well as a preparation method and application thereof, and belongs to the technical field of biological detection and biological engineering. The rabbit monoclonal antibody for resisting the human phosphorylated tau217 comprises a light chain variable region and a heavy chain variable region, and the monoclonal antibody comprises HZK33-B0010, HZK33-B0011 and HZK33-B0029. The invention develops three rabbit monoclonal antibodies for identifying p-tau217, the rabbit monoclonal antibodies do not have cross reaction with non-phosphorylated tau protein or phosphorylated tau protein at other sites, the rabbit monoclonal antibodies can be combined with p-tau217 with high specificity, and a related body fluid marker detection kit with higher specificity is developed on the basis of the rabbit monoclonal antibodies.
Owner:UNIV OF SCI & TECH OF CHINA
Method for preparing ginsenoside preparation by biological engineering technology
The invention discloses a method for preparing the ginsenoside preparation by biological engineering technology, wherein the ginsenoside preparation comprises at least one of the saponin-enriched preparation and the saponin-enriched syrup; the compound bio-enzyme preparation prepared by the combined bacteria preparation of yeast and lactic acid bacteria provided by the invention is rich in amino acid residues, amides, coenzymes and active groups such as lactic acid and linoleic acid; and the ginsenoside preparations containing ginsenosides can be prepared by adding monosaccharides or polysaccharides to the compound bio-enzyme preparations, providing new directions and ideas for obtaining ginsenosides and their products.
Owner:MINGZHIYUAN (HANGZHOU) BIOLOGICAL TECH CO LTD
Biochemical incubator
ActiveCN224299203Ueffective temperatureStable culture conditionsBioreactor/fermenter combinationsBiological substance pretreatmentsAir filtrationInsulation layer
The utility model relates to biological engineering equipment technical field, concretely relates to a biochemical incubator, including base, is equipped with the box on the base, is fixed in the box incubator, is pasted with the conduction sheet on the incubator, and the semiconductor refrigeration heating module is connected with the conduction sheet, and one side of the box is equipped with air filtration system, and is communicated with the incubator through the circulation pipe, and the ultrasonic humidifier is equipped below the semiconductor refrigeration heating module, and is communicated with the incubator through the pipeline, and the incubator is equipped with the sealed door, and there is the gap between the incubator and the box and forms the heat insulation layer, and the controller is connected with the temperature and humidity sensor group, gas sensor group electric signal, can automatically regulate and control each module according to real -time data.
Owner:贵州中科分子生物有限公司
Application of corn Zm00001d028750 gene in regulation and control of grain traits
The invention discloses application of a corn Zm00001d028750 gene in regulation and control of grain traits, and relates to the technical field of bioengineering, a nucleotide sequence of a coding region of the Zm00001d028750 gene is as shown in SEQ ID NO.1, and an amino acid sequence of a protein coded by the Zm00001d028750 gene is as shown in SEQ ID NO.2. The invention further discloses application of the corn Zm00001d028750 gene in regulation and control of grain traits and application of the corn Zm00001d028750 gene in regulation and control of grain traits. According to the application of the corn Zm00001d028750 gene in regulation and control of grain traits, the hundred-grain weight of corn is an important factor influencing the yield of corn, and along with the increase of the hundred-grain weight of corn, the higher the seed yield is, the higher the yield is; lysine is an essential amino acid for human beings and monogastric animals, and the eating and feeding values of corn can be remarkably improved by increasing the content of lysine and total amino acids.
Owner:EDGENE BIOTECHNOLOGY (WUHAN) CO LTD
Isatchenkia orientalis for efficiently synthesizing L-malic acid and application thereof
The invention discloses issatchenkia orientalis capable of efficiently synthesizing L-malic acid and application of the issatchenkia orientalis, and belongs to the technical field of biological engineering. In an existing gene edited yeast strain in a laboratory, endogenous lactic dehydrogenase IoLDH2 and IoLDH3 are over-expressed, endogenous IoLDH1 is knocked out, and lactic dehydrogenase EcD-LDH from escherichia coli and lactic dehydrogenase NmD-LDH from neisseria meningitidis are over-expressed. After fermentation for 72 h in a 5 L fermentation tank, the accumulation amount of L-malic acid reaches 296.27 g / L, the yield is 0.97 g / g, the production intensity is 4.11 g / L / h, and the by-product lactic acid is remarkably reduced from 23.56 g / L to 6.7 g / L and is reduced by 71.56%.
Owner:JIANGNAN UNIV
Photoresponse hydrogel as well as preparation method and application thereof
The invention discloses photoresponse hydrogel as well as a preparation method and application thereof, and belongs to the technical field of intelligent materials. The preparation method specifically comprises the following steps: (1) synthesizing a compound 1; (2) synthesizing a compound 2; (3) dissolving acryloyl chloride in dichloromethane, dropwise adding into a dichloromethane mixed solution containing the compound 2 and triethylamine, stirring, washing, concentrating under reduced pressure, and recrystallizing; and (4) dissolving N, N-dimethylacrylamide, a phenylboronic acid monomer, diol, MEH and N, N '-methylene bisacrylamide in water, filling nitrogen, adding azodiisobutyronitrile, gelatinizing, and dialyzing, so as to obtain the hydrogel. The hydrogel based on visible light driven deformation is designed and prepared, controllable change of shape, volume or motion can be achieved through illumination, and the core application of the hydrogel comprises soft robot application, medical and biological engineering application, environment and energy application and other innovative application.
Owner:HUNAN INSTITUTE OF SCIENCE AND TECHNOLOGY
Method for improving citrus canker resistance by using CsENPP1 gene
The invention belongs to the technical field of molecular biology, and particularly discloses a method for improving citrus canker resistance by using a CsENPP1 gene, the coding protein of the CsENPP1 gene is citrus nucleotide pyrophosphatase / phosphodiesterase 1, the coding sequence of the CsENPP1 is a nucleotide sequence shown as SEQ ID No.1, and the citrus canker resistance is improved based on overexpression of the CsENPP1 gene in citrus cells. The citrus nucleotide pyrophosphatase / phosphodiesterase 1 coding gene is integrated into citrus through an expression vector, the canker attack degree of the obtained transgenic material can be reduced to 48.9% of that of the existing citrus to the maximum extent, the canker attack degree can be remarkably reduced, the scab area is reduced, and the yield of the citrus is improved. The technology provided by the invention is a bioengineering technology which has potential to improve the citrus canker resistance, and has great value for citrus canker resistance molecular breeding.
Owner:GERMPLASM INNOVATION GRAND SCIENCE CENTER OF WESTERN CHINA (CHONGQING) SCIENCE CITY
Application of gene ZmNPF6.8 in regulation and control of corn nitrogen absorption and utilization efficiency and corn single plant yield
The invention relates to the technical field of biological engineering, in particular to application of a gene ZmNPF6.8 in regulation and control of corn nitrogen absorption and utilization efficiency and single-plant yield of corn. The gene ZmNPF6.8 has the following characteristics: 1) a coding region nucleotide sequence as shown in SEQ ID NO: 3; and 2) a nucleotide sequence which has more than 90% of homology with the nucleotide sequence as shown in SEQ ID NO: 3 and forms protein with the same function as the nucleotide sequence as the nucleotide sequence as shown in SEQ ID NO: 3. According to the invention, the expression quantity of the gene ZmNPF6.8 can be down-regulated by inserting a Mutuator transposon into an amino acid coding region of the gene, and meanwhile, the nitrogen absorption and utilization efficiency and the yield per plant of corn are remarkably reduced. Researches show that the nitrogen absorption and utilization efficiency of the corn and the change of the yield of a single plant of the corn can be regulated and controlled by changing the expression quantity of the gene ZmNPF6.8, and the gene is of great significance to the high-efficiency and high-yield breeding work of the corn in the future.
Owner:JIANGSU ACAD OF AGRI SCI
Hunan millet tissue culture regeneration system, culture method and application
The application discloses a tissue culture regeneration system and culture method of Hunan millet, and relates to the field of biological engineering. The regeneration system is to take the Hunan millet seeds with removed hulls as explants, and utilizes a culture medium composed of 1 / 2MS basic culture medium, 0.6-0.99 mg / L 2,4-D, 30 g / L sucrose, 7 g / L agar and 0.1% PPM, with pH being 5.8, to make the explants form complete Hunan millet plants. The regeneration system is used for tissue culture of the Hunan millet, effectively avoiding the problems of long culture period, high requirement for personnel and high culture cost caused by diversification of culture medium configuration in large-scale factory production. The application provides a simpler and more efficient method for large-scale factory production, research on gene function and molecular breeding and the like.
Owner:INSTITUTE OF ANIMAL SCIENCES OF CHINESE ACADEMY OF AGRICULTURAL SCIENCES
Mxene-based photothermal coupling composite film for bone repair and preparation method thereof
This invention belongs to the field of biological engineering technology, specifically relating to an MXene-based photothermal coupling composite film for bone repair and its preparation method; comprising MXene, type I collagen, and gelatin solution, obtained by sequential bridging and cross-linking with FeCl3 solution; wherein the mass ratio of MXene to type I collagen is (3-9):(1-7), and gelatin accounts for 3.6%-10.4% of the final composite material mass; the preparation method includes: ultrasonically dispersing MXene and mixing it with gelatin solution, then compounding it with type I collagen solution, vacuum filtering and drying to form a matrix film, and finally cross-linking it by impregnation with FeCl3 solution; this film has mechanical properties matching natural bone tissue, good biocompatibility, and high-efficiency photothermal conversion capability under near-infrared light irradiation, and is suitable for bone defect repair and photothermal synergistic therapy.
Owner:NANJING STOMATOLOGICAL HOSPITAL
An acetylacetone cleavage enzyme mutant and a method for producing 2-acetylcyclohexanone by an extracellular enzyme reaction
The application discloses an acetylacetone cleavage enzyme mutant and a method for generating 2-acetylcyclohexanone through an extracellular enzyme reaction, and belongs to the technical field of biological engineering. In order to solve the technical problem of how to improve the yield of 2-acetylcyclohexanone generated through an extracellular enzyme reaction, the problem of limited Dke1 substrate types in a whole-cell synthesis system is solved, and the toxicity of 2-acetylcyclohexanone to cells is inhibited. The application provides an acetylacetone cleavage enzyme mutant, wherein the mutant is obtained by mutating the amino acid at the 105th position of the acetylacetone cleavage enzyme with the amino acid sequence shown in SEQ ID NO. 3 to alanine from glycine, and the yield of 2-acetylcyclohexanone generated through an enzyme reaction of the mutant in vitro reaches 14.22 mg / L.
Owner:QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
Method for constructing nasal mucosa epithelium single-layer model by using organoid digested by single cell
PendingCN122038278ACell dissociation methodsMicrobiological testing/measurementEpitheliumCell adhesion
The invention discloses a method for constructing a nasal mucosa epithelium single-layer model by using a single-cell digested organoid, and belongs to the technical field of bioengineering. The method comprises the following steps: firstly, carrying out digestion treatment, red blood cell lysis treatment, inoculated culture and passage on nasal mucosa tissues to obtain nasal mucosa organoid, then adding recombinase for dissociation to obtain dissociated epithelial cells, finally, planting the dissociated epithelial cells in a culture container subjected to coating treatment for incubation and adherence, and continuing to culture to obtain the nasal mucosa organoid. And preparing the nasal mucosa epithelium single-layer model. The multi-lineage differentiation potential and individual specificity of the organoid-derived cells are reserved, the cell adherence, passage and cryopreservation recovery stability is remarkably improved, the culture period is shortened, reagent expenditure and time cost are saved, and the defects that a traditional cell line is poor in physiological correlation, difficult in primary and gas-liquid interface culture acquisition, long in period and low in cost are overcome. And the three-dimensional organ-like chamber structure is not beneficial to top surface observation and solute transport research, so that the three-dimensional organ-like chamber structure is more suitable for scientific experiments.
Owner:NINGXIA MEDICAL UNIVERSITY GENERAL HOSPITAL
Glycosyltransferase, nucleic acid fragment, recombinant expression vector, host cell and application thereof
The invention relates to glycosyltransferase, a nucleic acid fragment, a recombinant expression vector, a host cell and application of the glycosyltransferase. The amino acid sequence of the glycosyl transferase is as shown in SEQ ID NO: 1. According to the application, an enzyme for converting 3-oxo-ionol glycosyl into 3-oxo-ionol glucoside is found from tobacco, namely, a novel glycosyl transferase is provided, and the function of the enzyme in biosynthesis of related glycosyl transferase genes is cloned and verified for the first time; in vitro, the recombinant protein can be used for efficiently converting a 3-oxo-ionol substrate into corresponding glycoside. The invention further provides a recombinant plasmid containing the glycosyl transferase gene, glucoside can be massively synthesized through a bioengineering method, meanwhile, the invention further provides application of the recombinant plasmid capable of massively synthesizing 3-oxo-ionol glucoside, more basic elements are provided for synthetic biology of natural products, and the recombinant plasmid can be used for preparing a large amount of 3-oxo-ionol glucoside. In addition, guidance and basis are provided for rational design of the enzymes, and the method has good industrialization prospects.
Owner:SICHUAN SANLIAN NEW MATERIAL CO LTD +1
Rat tex264 recombinant protein, recombinant vector, polyclonal antibody and application
ActiveCN120173088BCell receptors/surface-antigens/surface-determinantsSerum immunoglobulinsImmunofluorescenceWestern blot
The application provides a rat TEX264 recombinant protein, a recombinant vector, a polyclonal antibody and application, and relates to the technical field of biological engineering.The amino acid sequence of the rat TEX264 recombinant protein is shown as SEQ ID NO.2.The application provides a preparation method of an anti-rat TEX264 polyclonal antibody, and a polyclonal antibody against rat TEX264 is prepared; the antibody can specifically recognize endogenous TEX264 protein of a rat, and is used for experiments such as ELISA, Western Blot, immunofluorescence analysis and the like.The application provides a material basis for studying the function of TEX264.The antibody of the application can detect TEX264 protein of human and mouse tissues in addition to recognizing and detecting rat TEX264, compared with a related antibody on the market which can only detect TEX264 protein of mouse and / or human tissue samples; the antibody can simultaneously detect TEX264 protein of rat, mouse and human tissue samples, and is suitable for various different detection methods such as Western Blot, immunohistochemistry and immunofluorescence, and has advantages in economic benefits.
Owner:THE FIRST MEDICAL CENT CHINESE PLA GENERAL HOSPITAL
Engineering probiotic EcN-Vh as well as construction method and application thereof
PendingCN121472275APolypeptide with localisation/targeting motifBacteriaTumor reductionBio engineering
The invention provides engineering probiotics EcN-Vh as well as a construction method and application thereof, and belongs to the technical field of biological medicines. According to the present invention, the engineering probiotic capable of expressing the BTApep-TAT is constructed through the bioengineering technology, the BTApep-TAT is expressed through the plasmid integration mode, the release of the peptide is achieved by using the arabinose-induced cracking system, and the engineering probiotic is used for the in-vivo sustainable production of the BTApep-TAT. The engineering probiotic EcN-Vh disclosed by the invention can continuously generate an inhibitory peptide BTApep-TAT of a targeted BORIS (Bovine Biosensor), and is used for treating the colorectal cancer. According to the probiotics, by inhibiting ADP-ribosylation and Wnt / beta-catenin signal channels of BORIS, the occurrence rate and progress of tumors are remarkably reduced, and a novel, safe and efficient probiotic delivery system is provided for treatment of colorectal cancer.
Owner:ZHEJIANG MEDICAL COLLEGE
Genetically engineered bacterium for catalyzing synthesis of ornamental blue from ornamental blue derivative as well as construction method and application of genetically engineered bacterium
The invention discloses a genetically engineered bacterium for catalyzing synthesis of ornamental blue from an ornamental blue derivative as well as a construction method and application of the genetically engineered bacterium, and relates to the technical field of bioengineering. According to the invention, on the basis of a gene editing technology, CobB, NOX, katE, NAMPT, nadD, rbsk, prs and ppk genes are integrated onto an escherichia coli genome; a genetically engineered bacterium for catalyzing synthesis of the observation blue derivative (N-acetylobservation blue or N, N '-diacetyl-observation blue) is constructed, synthesis of NAD + can be enhanced, cyclic regeneration of NAD + can be realized, H2O2 which is generated in the synthesis process of NAD + and has a toxic effect on enzyme is eliminated, supply of cofactors PRPP and ATP is enhanced, deacetylase is activated, and the observation blue derivative (N-acetylobservation blue or N, N'-diacetyl-observation blue) is enabled to be converted into the observation blue derivative (N-acetylobservation blue or N, N '-diacetyl-observation blue) into the observation blue derivative (N-acetylobservation blue or N, N'-diacetyl-observation blue). N, N '-diacetyl-observation blue) is used for catalytic synthesis of observation blue.
Owner:VERTEXYN BIOWORKS CO LTD
Application of inhibitor acting on NOS1 related ceRNA in relieving sheep endometritis caused by LPS
The invention discloses application of an inhibitor acting on NOS1 related ceRNA in relieving sheep endometritis caused by LPS and promoting embryo implantation, and relates to the field of bioengineering. The inhibitor is si-NOS1 (SEQ ID No.1) and / or si-TCONS00000014 (SEQ ID No.2), and by regulating and controlling a NOS1 / bta-miR-370 / TCONS00000014 signal axis, the endometrial oxidative stress excessive activation induced by LPS (lipopolysaccharide) is improved, the autophagy and apoptosis of cells are relieved, and the expression of a key implantation gene is recovered. Experiments prove that the inhibitor can significantly improve the sheep embryo implantation rate and reduce the embryo loss probability, provides a new target and strategy for preventing and treating LPS-induced sheep endometritis and embryo implantation disorder, and has important significance for improving the sheep breeding efficiency.
Owner:NORTHEAST AGRICULTURAL UNIVERSITY
Method for producing agricultural tryptophan through fermentation and application
The invention relates to the technical field of biological engineering, and discloses a method for producing agricultural tryptophan through fermentation and application, and the method comprises the following steps: after thalli are accumulated, adding L-serine in a pulse manner, switching to double-carbon-source material supplementation of glucose and glycerol, and starting energy metabolism uncoupling control at the same time; according to the energy metabolism decoupling control, a decoupling agent is fed, a real-time respiration quotient is used as a feedback signal, and a closed-loop system is used for automatically adjusting the flow acceleration rate, so that the respiration quotient is maintained in a target range. According to the invention, intracellular energy charge is reduced through uncoupling so as to relieve glycolysis inhibition and strengthen carbon flux, double carbon sources are utilized to maintain redox balance, and pulse feeding is matched to eliminate terminal synthesis bottleneck. According to the method, the metabolic competition problem of cell growth and product synthesis is effectively solved, the conversion rate and fermentation yield of tryptophan are effectively improved, and the obtained product is suitable for preparing an agricultural fertilizer synergist or a feed additive.
Owner:YANTAI HONGYUAN BIOLOGICAL FERTILIZER CO LTD
An acetylacetone cleavage enzyme mutant and a method for producing 3-methyl-2,4-pentanedione by an extracellular enzyme reaction
The application discloses an acetylacetone cleavage enzyme mutant and a method for generating 3-methyl-2,4-pentanedione by extracellular enzyme reaction, and belongs to the technical field of biological engineering. The application solves the problems of Dke1 substrate type inhibition in a whole cell synthesis system and the problem of 3-methyl-2,4-pentanedione toxicity inhibition on cells, and aims to improve the yield of 3-methyl-2,4-pentanedione. The application provides an acetylacetone cleavage enzyme mutant, wherein the mutant is obtained by mutating the amino acid at the 117th position of acetylacetone cleavage enzyme with the amino acid sequence shown in SEQ ID NO. 3 to glycine from methionine, and the amino acid sequence of the mutant is shown in SEQ ID NO. 3. The acetylacetone cleavage enzyme mutant can convert 2,3-butanedione and acetic acid into 3-methyl-2,4-pentanedione by enzyme reaction in vitro, and the yield reaches 23.29 mg / L.
Owner:QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
Sample data generation method and device, equipment and storage medium
The invention provides a sample data generation method and device, equipment and a storage medium, which can be applied to various scenes such as biological engineering and biological pharmacy. The method comprises the following steps: acquiring sequence data of a first biomolecule and structural data of the first biomolecule and a second biomolecule; determining a group belonging to a binding site in groups included in the structural data of the first biomolecule based on the spatial information of the first biomolecule and the second biomolecule; labeling whether each group included in the sequence data of the first biomolecule belongs to a binding site or not based on the group belonging to the binding site in the groups included in the structural data of the first biomolecule to obtain label information of the first biomolecule; and on the basis of the tag information of the first biomolecule, the sample data corresponding to the first biomolecule is generated, so that the quality of the sample data is improved, the labor cost is low, the efficiency is high, and large-scale and standardized data samples can be obtained.
Owner:TENCENT TECHNOLOGY (SHENZHEN) CO LTD
Method for improving antioxidation and hypoglycemic activity of bovine bone enzymatic hydrolysate
The invention belongs to the technical field of food science and bioengineering, and provides a method for improving antioxidant and hypoglycemic activity of bovine bone enzymatic hydrolysate. The method comprises the following steps: performing ultrasonic pretreatment on cattle bone powder (500W, 30min), performing alkaline protease hydrolysis (50 DEG C, 5h), mixing the inactivated cattle bone powder with D-xylose (8: 1), and performing Maillard reaction (100 DEG C, 120min). The DPPH free radical scavenging rate and the ABTS free radical scavenging rate of the obtained product respectively reach 91% and 88.13%, and the antioxidant activity is remarkably improved; the synthetic peptide, such as FGFDGDFYR, has both the hydroxyl radical scavenging rate of 74.52% and the alpha-glucamylase inhibition rate of 70.2%, so that the double-function activity of resisting oxidation and reducing blood sugar is realized; meanwhile, the product is enhanced in palatable taste and reduced in bitter taste, is rich in meat flavor compounds (such as 2-methyl-3-furanmercaptan), has flavor and functional advantages, and promotes high-value utilization of bovine bones.
Owner:QINGDAO AGRI UNIV
Construction method and application of escherichia coli capable of producing fumaric acid
The invention discloses a construction method and application of escherichia coli capable of producing fumaric acid, and belongs to the technical field of biological engineering. By-product synthesis related genes such as a fumarase coding gene fumA, a lactic dehydrogenase coding gene ldhA and a pyruvate oxidase coding gene poxB are knocked out, so that the yield of fumaric acid is effectively increased, and the content of heteroacid is reduced. On the basis, the expression of Afpyc, Ecppc and EcaceA genes is up-regulated independently or in a combined manner. The genetically engineered bacterium constructed by the invention adopts an aerobic-microaerobic two-stage fermentation strategy in a 5L fermentation tank, the highest yield of fumaric acid reaches 50.2 g / L after fermentation for 60 hours, the glucose conversion rate is 0.48 g / g, and the by-products lactic acid and acetic acid are both controlled to be 4-5g / L. The strain does not need to carry plasmids, so that the risks of antibiotic use and plasmid loss are avoided, and the strain has good industrial application potential.
Owner:JIANGNAN UNIV
A method for preparing a specific n-acetyl chitosan
The application belongs to the technical field of marine biological engineering, and particularly relates to a preparation method of specific N-acetylated chitosan (N-acetylated chitosan with different acetylation degrees). Specifically, the method comprises the following steps: taking fully deacetylated chitosan as a substrate, and performing reaction on the substrate in sodium acetate buffer solution through chitin deacetylase (PesCDA) with a specific deacetylation mode; and after the reaction, salt is removed, concentrated and freeze-dried, thereby obtaining chitosan with a specific N-acetylation degree. Compared with a traditional method for preparing chitosan with a specific N-acetylation degree, the method is green, environmentally friendly and pollution-free.
Owner:INST OF OCEANOLOGY - CHINESE ACAD OF SCI
Escherichia coli capable of efficiently expressing hydroxylated human collagen and construction method of escherichia coli
PendingCN121950652AIncreased hydroxylation levelsBacteriaMicroorganism based processesHydroxyprolineProline metabolism
The invention discloses Escherichia coli capable of efficiently expressing hydroxylated human collagen and a construction method of the Escherichia coli, and belongs to the technical field of biological engineering. According to the technical scheme, the engineered escherichia coli capable of efficiently expressing hydroxylated collagen is characterized in that an escherichia coli strain is modified as follows: at least one of proline metabolic pathway genes proA and proB in the escherichia coli strain is knocked out; and a collagen expression gene is introduced into a knocked-out Escherichia coli strain body. The invention develops a method for efficiently expressing hydroxylated human collagen, and the problems that an escherichia coli system cannot perform hydroxylation modification on exogenous recombinant protein, hydroxyproline in a culture medium is directly utilized, and the modification efficiency is low when a traditional strain co-expresses P4H are systematically solved.
Owner:YEASEN BIOTECHNOLOGY (SHANGHAI) CO LTD
L-2, 4-diaminobutyric acid transaminase mutant 2423-EctB-H and application thereof
The invention discloses an L-2, 4-diaminobutyric acid transaminase mutant 2423-EctB-H and application of the mutant 2423-EctB-H, and belongs to the technical field of biological engineering. The amino acid sequence of the mutant is as shown in SEQ ID NO.1, the coding gene of the mutant is 2423-ectB-H, and the nucleotide sequence of the mutant is as shown in SEQ ID NO.2. Compared with the original L-2, 4-diaminobutyric acid transaminase, the L-2, 4-diaminobutyric acid transaminase mutant 2423-ectB-H provided by the invention has the advantages that the activity is enhanced, and the efficiency of catalytically generating L-2, 4-diaminobutyric acid by taking L-aspartic acid-beta-semialdehyde as a substrate is obviously improved; the strain is inserted at the position of the escherichia coli thrA gene and is used for fermenting ectoine, and the fermentation content of ectoine can be remarkably increased.
Owner:SHANDONG FREDA BIOTECH
Purification method of phosphoryl diamine morpholino oligonucleotide
The invention provides a method for purifying phosphoryl diamine morpholino oligonucleotide, and relates to the technical field of oligonucleotide purification. The purification method of the phosphoramide morpholino oligonucleotide comprises the following steps: carrying out ultrafiltration liquid change for the first time, carrying out ion pair reversed phase chromatography, carrying out ultrafiltration liquid change for the second time, and freeze-drying, so as to obtain the high-purity phosphoramide morpholino oligonucleotide. According to the invention, a purification process route of the phosphoric acid diamide morpholino oligonucleotide (PMO) is developed, the purity of the phosphoric acid diamide morpholino oligonucleotide (PMO) is effectively improved, the industrial requirements can be accurately met, and the development of the fields of scientific research and bioengineering is promoted.
Owner:HANGZHOU APEXTIDE BIOMEDICAL TECHNOLOGY CO LTD
Yellow wine vinasse polypeptide capable of enhancing Kokumi flavor and promoting health as well as preparation method and application of yellow wine vinasse polypeptide
The invention belongs to the technical field of vinasse functional development, and particularly relates to yellow wine vinasse polypeptide with Kokumi flavor enhancement and health promotion functions as well as a preparation method and application. The yellow wine vinasse polypeptide provided by the invention is prepared by the following steps: firstly, identifying three active peptides RFY, QMGR and AGRR which have strong binding capacity with a calcium sensitive receptor (CaSR) and glucolipid metabolism related enzymes from yellow wine vinasse by using high-throughput screening and molecular docking technologies, and synthesizing the three polypeptides through chemical synthesis methods such as a solid-phase synthesis method or a bioengineering method after obtaining amino acid sequences RFY, QMGR and AGRR. Sensory evaluation, an electronic tongue test and an in-vitro / in-vivo glycolipid metabolism related enzyme inhibition and oxidation resistance test are carried out, so that the three polypeptides are proved to be the multi-effect yellow wine vinasse polypeptides with Kokumi flavor enhancement and health promotion, and the technical problem of lack of the multi-effect yellow wine vinasse polypeptides in the prior art is solved.
Owner:GUANGDONG UNIV OF TECH
A parentage identification method for procambarus clarkii based on four-base microsatellite fluorescence multiplex PCR
ActiveCN114959050BMicrobiological testing/measurementClimate change adaptationBiotechnologyMultiplex
The application provides a kind of based on four base microsatellite fluorescence multiplex PCR red claw crayfish parentage identification method, belong to biological engineering technical field, the application includes the following steps: (1) extracting the genome DNA of sample to be measured;(2) the screening of red claw crayfish microsatellite primer (3) the construction of multiplex PCR amplification system 20 pairs of primers in step (2) are grouped according to 120-200bp, 220-260bp, 280-340bp, 360-420bp, 440bp-500bp interval, select amplification combination, obtain multiple multiplex PCR amplification system;(4) parentage identification sample genome DNA amplification, obtain multiplex PCR product, typing, read parent and offspring genotype, judge parent-child relationship.The application can realize that there is no interaction between multiple primers, no non-specific amplification, do not make the amplified product overlap.In the process of PCR amplification, multiple target fragments are amplified simultaneously, which can save experimental samples, experimental cost, improve experimental efficiency, and achieve 100% parentage identification success rate for red claw crayfish.
Owner:ZHEJIANG INST OF FRESH WATER FISHERIES