133 results about "Glutaminase" patented technology

The invention provides an enzymolysis preparation method of a milk-derived taste enhancing peptide, which comprises the following steps: carrying out enzymolysis on a liquid dairy product by using flavourzyme and glutaminase, and then carrying out enzyme deactivation to obtain an enzyme-deactivated product; carrying out acidification centrifugation and ultrafiltration on the enzyme-deactivated product, and freeze-drying the separated effluent to obtain freeze-dried powder; and loading the freeze-dried powder, and separating by a glucose gel column. The product disclosed by the invention does not generate bitter taste, and compared with a sample which is not subjected to enzymolysis, the flavor level of an original skimmed milk product is enriched, and the sample subjected to enzymolysis has the mellow taste of caramel and frankincense. The method comprises the following steps: separating milk-derived taste active peptides from milk-derived taste active peptides, predicting delicate flavor, anti-oxidation and anti-inflammatory activity and ACE blood pressure lowering functional activity of separated peptide liquid through a model, performing taste verification on the peptides predicted to have taste activity through Fmoc solid-phase synthesis, and performing sensory verification on the milk-derived taste active peptides LSFD, EDIKQME, IKQMEAE and TEDELQDK after synthesis, so that the milk-derived taste active peptides are obtained for the first time and are subjected to sensory verification.

The invention provides a composition for reducing uric acid and relieving gout and a preparation method thereof, and relates to the technical field of products for reducing uric acid and relieving gout. The composition comprises the following raw materials: 18-24 parts of phellinus igniarius, 9-13 parts of sunflower disc small molecule peptide extract, 3-7 parts of corn stigma, 4-6 parts of poria cocos, 2-5 parts of pawpaw, 3-6 parts of liquorice, 2-5 parts of astragalus extract and 3-6 parts of lettuce extract. The preparation method of the sunflower disc small-molecule peptide extract comprises the following steps: carrying out enzymolysis on sunflower disc powder twice, carrying out membrane filtration to obtain a sunflower disc small-molecule peptide solution, reacting the sunflower disc small-molecule peptide solution with chitosan oligosaccharide and transglutaminase, and carrying out freeze drying to obtain the sunflower disc small-molecule peptide extract. Through the proper raw material composition and proportion, the metabolic function can be effectively regulated to promote excretion of uric acid, so that the content of xanthine oxidase and adenosine deaminase in the liver is obviously reduced, the uric acid level is effectively reduced, the intestinal probiotic structure can be stabilized, the immunity is enhanced, the kidney is protected, and the good effects of reducing uric acid and relieving gout are achieved.

The invention relates to the field of biomedical application of inorganic materials, in particular to application of lanthanum carbonate in preparation of a medicine for treating hepatic encephalopathy. According to the application of lanthanum carbonate in preparation of the medicine for treating hepatic encephalopathy, the medicine comprises lanthanum carbonate and pharmaceutically acceptable auxiliary materials, the lanthanum carbonate comprises any one or more of lanthanum carbonate hydrate and basic lanthanum carbonate, the medicine is ground and then mixed with food to prepare a medicine mixture, and the medicine mixture is prepared into the medicine for treating hepatic encephalopathy. The mass ratio of the lanthanum carbonate in the medicine mixture is 1-10%. According to the application, the blood ammonia is reduced based on the glutaminase, the hepatic encephalopathy is reversed by reducing the blood ammonia and systemic inflammation, and the liver function is recovered by regulating the inflammation, so that the hepatic encephalopathy is favorably cured, a good treatment effect is shown, and a new candidate medicine is provided for clinically treating the hepatic encephalopathy.

The invention discloses a preparation method and application of a myofibrillar protein emulsion based on enzymatic modification. According to the technology, a myofibrillar protein aqueous suspension is subjected to enzymatic deamidation and glycosylation reaction through protein glutaminase and glucan, a protein-polysaccharide compound with good emulsifying performance is constructed, soybean oil serves as an oil phase, and emulsion systems with different oil phase volume fractions are constructed through a controllable homogenizing technology. The obtained emulsion has the characteristics of natural degradability, excellent rheological property, uniform particle size distribution, long-term physical stability and the like. Compared with a traditional chemical modification technology, the technology adopts a whole bio-based raw material combined enzyme method green technology, the problems of organic solvent residues and the like are effectively solved, and the production cost is reduced while the product safety is ensured. The emulsion has remarkable application advantages in the fields of functional food active ingredient delivery, drug controlled release carriers, cosmetic transdermal absorption systems and the like, and a new thought is provided for development of a biomacromolecule-based delivery system.

The invention belongs to the technical field of protein processing, and particularly relates to modified beta-lactoglobulin as well as a preparation method and application thereof. The modified beta-lactoglobulin is a composite modified product which is obtained by performing a papain hydrolysis treatment process on beta-lactoglobulin to obtain a hydrolysate and then performing glycosylation modification on the hydrolysate by adopting chitosan oligosaccharide and transglutaminase. The product has significantly improved oxidation resistance, emulsifying properties and foaming activity, and is suitable as a food processing ingredient, such as an antioxidant, an emulsifier or a foaming agent.

The invention relates to a flame-retardant skin-friendly polyurethane sponge with in-situ reaction and bio-enzyme catalysis synergism. The water-based polyurethane foam is prepared from the following components: polyether polyol, diphenylmethane diisocyanate or toluene diisocynate, a chain extender, a high-efficiency foam stabilizer, a high-efficiency composite catalyst, water and ethanolamine phosphate, ethanolamine phosphate is added into water in advance, and then all the raw materials are mixed for polymerization foaming; after curing, spraying a spraying liquid consisting of phosphoethanolamine, regenerated protein and transglutaminase to generate a nano protein film, and synchronously performing vacuum suction to keep sponge pores unblocked. The preparation method has the advantages that a small amount of ethanolamine phosphate is added before polymerization foaming to serve as a phosphorus-nitrogen synergistic flame retardant and also serve as a catalytic cross-linking agent to participate in a reaction during polymerization, biological enzyme is added for catalysis after curing is finished, and the skin-friendly PU sponge with excellent flame retardance is prepared through in-situ polymerization reaction and biological enzyme enzymatic polymerization cooperation, and the sponge performance is not affected; the raw materials of the flame retardant come from biomass products and are environment-friendly and safe.

The invention relates to a high-yield protein glutaminase Chryseobacterium prion strain and a preparation method thereof. The strain is obtained by carrying out ARTP and NTG compound mutagenesis treatment on an original strain of Chryseobacterium prion and carrying out high-throughput screening on the strain subjected to the compound mutagenesis treatment. The strain is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC M 20251792. The invention also relates to a method for preparing protein glutaminase by using the strain of the invention. The enzyme activity of the protein glutaminase of the strain is 6 times that of an original strain, and the production efficiency of the protein glutaminase can be remarkably improved.

The invention provides bacillus licheniformis for high-yield protein glutaminase as well as a construction method and application of the bacillus licheniformis, a target strain is obtained by knocking out an alkaline protease gene aprE and extracellular protease genes bpr, epr, vpr or wpr and heterologously expressing the protein glutaminase gene, an EPE strain is optimal in expression, PG enzyme activity reaches 0.358 U / mL and is improved by 3.55 times compared with that of a wild type, and the bacillus licheniformis has the advantages that the bacillus licheniformis is high in yield and high in protein glutaminase yield. In addition, the growth condition of each mutant strain is not obviously abnormal, and the growth speed is obviously increased. It is fully proved that the expression level of the heterologous protein PG can be remarkably improved by targeted knockout of the extracellular protease gene of the bacillus licheniformis, and a theoretical basis is laid for construction of an efficient protein expression host. The invention not only provides a new scheme for high-efficiency expression of PG, but also provides important reference for expression optimization of heterologous proteins of other gram-positive bacteria, and shows a wide industrial application prospect.

The invention provides a preparation method and application of myofibrillar protein hydrosol, and the method comprises the following steps: uniformly dispersing myofibrillar protein in water to obtain a myofibrillar protein suspension, mixing the myofibrillar protein suspension with protein glutaminase to obtain a mixed solution A, mixing the mixed solution A with a glucan aqueous solution to carry out glycosylation reaction to obtain a mixed solution B, and carrying out freeze drying to obtain the myofibrillar protein hydrosol. And cooling in an ice-water bath, and storing at 4 DEG C to obtain the myofibrillar protein hydrosol. The myofibrillar protein hydrosol is used for preparing protein beverages and nasal feeding nutrient solutions. The method for preparing the myofibrillar protein hydrosol with excellent dispersity and storage stability at lower production cost under the condition that professional large-scale instruments and equipment are not needed is used for preparing protein beverages and nasal feeding nutrient solutions.

The invention discloses a non-phosphorus green composite water holding agent for improving the quality of chicken feet and an application method of the non-phosphorus green composite water holding agent, and belongs to the technical field of food processing. The raw materials of the non-phosphorus green composite water-holding agent comprise a dissociation water-holding agent and a crosslinking water-holding agent, and sodium bicarbonate provides an alkaline environment, so that protein is negatively charged, and electrostatic repulsion among carboxyl groups is increased. And collagen can be dissociated, the water binding capacity of meat is increased, the tenderness of the meat is improved, the bulkiness of the meat is increased, the protein network structure of the treated chicken feet is looser, and the water binding capacity is remarkably enhanced. Transglutaminase can catalyze cross-linking of glutamine and lysine in protein to form a tight three-dimensional network to physically wrap moisture; the protein structure can be stretched, hydrophilic groups are exposed, and the binding force with water is enhanced; meanwhile, combination of muscle fibers and collagen is strengthened, and the structure is stabilized. In addition, water loss caused by high temperature, freezing and the like in processing can be resisted, the succulent property and elasticity of meat are improved, and therefore the water-retaining property is improved.

The invention discloses a temperature-sensitive gel preparation containing a glutaminase inhibitor as well as a preparation method and application of the temperature-sensitive gel preparation, and belongs to the technical field of biological medicines. The temperature-sensitive gel preparation comprises outer-layer gel and inner-layer gel, the inner-layer gel is coated with the outer-layer gel, the outer-layer gel accounts for 40-75% of the total mass of the temperature-sensitive gel preparation, and the inner-layer gel accounts for 25-60% of the total mass of the temperature-sensitive gel preparation; the outer-layer gel is prepared from the following raw materials: an outer-layer gel matrix, a glutaminase inhibitor, a photosensitizer and a stabilizer; the raw materials of the inner-layer gel comprise an inner-layer gel matrix and an immune checkpoint inhibitor. According to the temperature-sensitive gel preparation, through photo-thermal control, the glutaminase inhibitor is firstly released to relieve the immunosuppression state, then the immune checkpoint inhibitor is released to kill tumors, and the glutaminase inhibitor and the immune checkpoint inhibitor act synergistically, so that efficient anti-tumor immunotherapy is achieved.

The invention belongs to the technical field of gene therapy and biological medicine, and particularly provides application of leucine in preparation of a medicine for preventing and / or treating aortic aneurysm. The leucine is independently used or is combined with other medicines for preventing and / or treating aortic aneurysm. The activity of GLUD1 is enhanced through leucine, so that conversion of Glu to alpha-KG is promoted, degradation of Glu is promoted, namely, the Glu level is reduced by enhancing degradation of Glu, and aortic aneurysm is improved. In a Glu metabolic network, Gln can be converted into Glu under the action of glutaminase 1 (GLS1), so that the generation of Glu is inhibited by inhibiting the activity of GLS1, and the effect of preventing and / or treating aortic aneurysm is achieved.

The application relates to the biomedical application field of inorganic materials, and particularly relates to application of lanthanum carbonate in preparation of a medicine for treating hepatic encephalopathy. The application relates to application of lanthanum carbonate in preparation of a medicine for treating hepatic encephalopathy, wherein the medicine comprises lanthanum carbonate and pharmaceutically acceptable adjuvants, and the lanthanum carbonate comprises any one or more of lanthanum carbonate hydrate and basic lanthanum carbonate. The application is based on the fact that glutamine enzyme can reduce blood ammonia, and the hepatic encephalopathy is reversed by reducing blood ammonia and systemic inflammation, the liver function is recovered by regulating inflammation, thereby helping to cure the hepatic encephalopathy, and good treatment effect is shown, so that a new candidate medicine for clinically treating the hepatic encephalopathy is provided.

The invention relates to the technical field of pharmaceutical preparations, in particular to mRNA (messenger ribonucleic acid) lipid nanoparticles and application thereof in treatment of triple-negative breast cancer. The invention constructs an mRNA lipid nanoparticle, breaks through the dilemma of single drug curative effect of a glucose transporter (GLUT) inhibitor and a glutaminase (GLS) inhibitor for triple negative breast cancer, and realizes double-track treatment of mRNA metabolism reprogramming and small molecule energy deprivation for the first time. The mRNA lipid nanoparticles enhance the sensitivity of triple negative breast cancer to small molecule energy deprivation treatment, so that the clinical transformation feasibility of BAY-876 and CB-839 is improved to a new dimension. The delivery efficiency of the mRNA lipid nanoparticles is high, the biological safety is good, meanwhile, the production process of the nanoparticles is simpler and more standard compared with plasmids and viruses, and the drug production cost is lower.

The invention relates to a Chryseobacterium-derived protein glutaminase PG3, a coding gene and an application thereof, and belongs to the technical field of biological genetic engineering. The amino acid sequence of the protein glutaminase PG3 is as shown in SEQ ID No. 1, and the cDNA sequence of the coding gene of the protein glutaminase PG3 is as shown in SEQ ID No. 2. A large amount of protein glutaminase PG3 from Chryseobacterium is prepared through an Escherichia coli expression system, and the protein glutaminase is used for carrying out enzymatic deamidation modification on the spotted silver carp myofibrillar protein PG3, so that the solubility of the spotted silver carp myofibrillar protein under a salt-free system condition is remarkably improved. The invention provides an important tool enzyme for protein deamidation modification, and lays a theoretical foundation for development of low-salt fish protein processed products.

This invention belongs to the fields of genetic engineering and enzyme engineering technology, and discloses a glutaminase mutant, immobilized glutaminase, and their applications. The glutaminase mutant is based on the amino acid sequence shown in SEQ ID NO.1, with amino acid mutations at the following sites: E113K, R136M, and H223V, and its amino acid sequence is shown in SEQ ID NO.3. Immobilized glutaminase prepared using the glutaminase mutant exhibits 5-10 times increased enzyme activity compared to the wild type. The substrate concentration in the synthesis of L-theanine catalyzed by the immobilized glutaminase reaches 80-150 g / L, with a conversion rate greater than 99%. It can be recovered through simple filtration and reused more than 5 times, with the conversion rate remaining essentially the same during repeated use, demonstrating high potential for industrial application.

The invention provides an enzyme-residue-free extracellular matrix microsphere as well as a preparation method and application thereof. An elutable sacrificial phase material capable of being subjected to liquid-liquid phase separation with an extracellular matrix is introduced in an extracellular matrix pelletizing process, and transglutaminase is confined in a sacrificial phase, so that the extracellular matrix microsphere is prepared. And the transglutaminase and the extracellular matrix form a spatially separated structure in the balling process. In the gel forming stage, transglutaminase catalyzes protein in an extracellular matrix on a two-phase interface to generate a cross-linking reaction, and microspheres with a three-dimensional network gel structure are formed; after cross-linking is completed, transglutaminase is removed by eluting the sacrificial phase material, and meanwhile, a porous network with a communicated structure is formed in situ in the microsphere, so that the obtained extracellular matrix microsphere still keeps good structural integrity and mechanical property while realizing no residue of enzyme with catalytic activity; the scaffold can provide stable support for cell adhesion, migration and three-dimensional culture, and is applicable to cell delivery, organoid construction, tissue engineering scaffolds and the like.

The present invention relates to glutaminases and their use in the manufacture of flavors for incorporation in foods. More particularly, the present invention relates to methods of making glutamate from protein sources and the use of such glutamate in foods.

Described herein are protein-payload conjugates and compositions thereof that are useful, for example, for target-specific delivery of therapeutic and / or imaging agent moieties. In certain embodiments, provided are specific and efficient methods for producing protein-payload constructs (e.g., antibody-drug conjugates) utilizing a combination of transglutaminase and Diels-Alder techniques. Antibody-drug conjugates and compositions which comprise glutaminyl-modified antibodies, Diels-Alder adducts, and reactive payloads and are provided.

The invention provides an application of a chickpea protein modified by protein glutaminase in preparation of a vegetable protein pudding. The invention further provides the vegetable protein pudding as well as a preparation method and application thereof. The composition for preparing the vegetable protein pudding comprises the chickpea protein modified by protein glutaminase and a grease compound. The chickpea protein modified by the protein glutaminase has high gel forming property, can be easily combined with grease compounds, and can be used for preparing the vegetable protein pudding rich in functional grease.

The invention relates to the field of medicinal chemistry, and particularly discloses an mTOR / GLS1 double-target inhibitor as well as a preparation method and medical application thereof. The compound is based on a rapamycin structure, an amide side chain containing pyridopyrimidine-thiadiazole substitution is introduced to a 42-O site, and through reasonable Linker connection, double-target synergistic inhibition on mammal rapamycin target protein (mTOR) and glutaminase 1 (GLS1) is realized. The compound can block glycometabolism and glutamine metabolism reprogramming of tumor cells at the same time, so that proliferation and metastasis of the tumor cells are remarkably inhibited, and drug resistance is reduced. In-vivo and in-vitro experiment results show that the compounds show excellent anti-proliferative activity in various breast cancer cell lines, and the representative compound I-4 has a relatively strong inhibition effect on mTOR and GLS1, can significantly inhibit tumor metastasis in an animal model, and has no obvious toxic or side effect. The mTOR / GLS1 double-target inhibitor provided by the invention can be used as an important candidate for developing novel efficient and low-toxicity antitumor drugs.

The application provides a longan peptide preparation method, product and application, and relates to the technical field of bioactive peptide processing. The longan peptide preparation method comprises the following steps: grinding longan kernels, adding water, and performing first enzyme hydrolysis to obtain an enzyme hydrolysate; the enzyme hydrolysate is inactivated, the pH value is adjusted to 8-9, stirring is performed at 30-40 DEG C, centrifugation and dialysis are performed, a supernatant is obtained, the pH value of the supernatant is adjusted to 4-6, and centrifugation is performed after standing to obtain longan kernel protein; the longan kernel protein is subjected to second enzyme hydrolysis, then inactivated, filtered, concentrated, and dried to obtain the longan peptide; the first enzyme hydrolysis is enzyme hydrolysis using cellulase, acid protease and glutamine enzyme; and the second enzyme hydrolysis is enzyme hydrolysis using trypsin, papain and carboxypeptidase. The longan peptide prepared by the application can effectively inhibit alpha-glucosidase, and achieve a sugar control effect.

The invention relates to methods of inhibiting hair growth in a subject, the method comprises administering an inhibitor of lactic acid fermentation to the subject, wherein the inhibitor is selected from the group consisting of pyruvate dehydrogenase kinase inhibitor, desoxyrhapontigenin, a phosphofructokinases inhibitor, a thioredoxin-1 inhibitor, L- Asparaginase, a GLUT1 inhibitor, a glutaminase C inhibitor, a hexokinase inhibitor and an mTORCI and mTORC2 inhibitor. Also provided herein are compositions for inhibiting hair growth and methods of screening for inhibitors of hair growth.

The present application relates to the field of cosmetic technology, and discloses a preparation process of cashmere goat bone-derived elastin and application thereof in cosmetics. The process comprises: defatting cashmere goat bone particles; using composite rumen microbial flora to perform bionic fermentation to destruct bone matrix and expose active sites; adopting neutral protease and specific elastase to perform stepwise directional enzymolysis; performing hydrophilic modification through transglutaminase catalysis of covalent anchoring of polyglutamic acid and elastin polypeptide; and finally, performing multi-stage membrane interception purification and drying. The present application realizes complete retention of the natural cross-linking structure of elastin under mild conditions, significantly improves water solubility, biological activity and transdermal permeability of the elastin, solves the problems of structural damage and pollution caused by traditional chemical methods, and provides a green path for high-value utilization of cashmere goat by-products.

The invention provides application of protein glutaminase to preparation of salt-reduced, fresh-increased and fishy-smell-removed fish protein gel products, and belongs to the field of food processing. A large amount of protein glutaminase PG3 derived from Chryseobacterium is prepared through a heterologous expression technology, the protein glutaminase PG3 is used for carrying out enzymatic deamidation modification on spotted silver carp MP, and finally, a spotted silver carp MP solution obtained after deamidation is heated and refrigerated to prepare the salt-free thermally-induced MP gel. In addition, the contents of substances with fishy smell, such as hexanal, nonanal and 1-octylene-3-alcohol, in the salt-free heat-induced MP gel are remarkably reduced, and the delicate flavor perception is improved. The method not only plays an important role in fishy smell removal and freshness enhancement of the fish protein gel, but also provides scientific basis and technical support for healthy and sustainable development of high-quality salt-reduced MP gel products.

The invention discloses a preparation method of a sugar-controlled glutinous rice product based on protein-starch molecule crosslinking, which comprises the following steps: pretreating glutinous rice to obtain glutinous rice flour; soybean protein and whey protein are compounded to prepare a protein mixed solution, transglutaminase is added for protein cross-linking, ascorbic acid is added for oxidation cross-linking modification, and modified compound protein is obtained; and adding glutinous rice flour and the modified compound protein into deionized water, adjusting the pH value, stirring at a constant temperature, shearing at high pressure, and freeze-drying to obtain the sugar-controlled glutinous rice product based on protein-starch molecule crosslinking. Nutrient enrichment and taste can be effectively considered, and the problem that a traditional glutinous rice product is deficient in protein is solved; meanwhile, a cross-linked network structure can lock moisture, the special soft, glutinous, sticky and elastic taste of the sticky rice is reserved, no protein peculiar smell or roughness exists, and the unification of blood glucose reduction, nutrition and taste is achieved.

A transglutaminase conjugate obtained by linking an active microorganism-derived transglutaminase precursor and an antibody-binding peptide compound; an antibody modifier containing the conjugate; and a method for producing an antibody-drug complex using the conjugate.

The invention relates to the technical field of microcapsule powder, in particular to microcapsule powder with a peptide-polysaccharide system and a preparation method. The microcapsule powder comprises the following components in percentage by mass: 2-3% of wall material reinforced particles; 3 to 5 percent of chitosan; 10 to 15% of whey protein; 1-2% of an emulsifier; 35 to 45% of maltodextrin; the content of transglutaminase is 0.05 to 0.1 percent; and the balance of functional grease. The functional grease comprises composite grease, a functional additive and a cross-linking agent in a mass ratio of (40-50): 5: 1; the preparation method of the microcapsule powder comprises the following steps: S1, preparing an inner-layer water phase; s2, preparing an oil phase; s3, preparing an outer layer water phase; s4, preparing a water-in-oil-in-water emulsion with a peptide-polysaccharide system; s5, preparing micro-capsule powder; the microcapsule powder disclosed by the invention has a water-in-oil-in-water structure, so that the stability is remarkably improved; then a compact'peptide-polysaccharide 'network structure is formed through transglutaminase, the interfacial tension is reduced, thermal denaturation is resisted in the subsequent spray drying process, and the embedding rate is increased.

A method to prepare a mixture to make cereal-based food products which natively contain gluten includes a step of mixing cereal flour and a step of preparing a mixture obtained by mixing a hydrolyzed flour composition with a transglutaminase enzyme and with a source of lysine.