28 results about "Glutaminase" patented technology

The invention relates to methods of inhibiting hair growth in a subject, the method comprises administering an inhibitor of lactic acid fermentation to the subject, wherein the inhibitor is selected from the group consisting of pyruvate dehydrogenase kinase inhibitor, desoxyrhapontigenin, a phosphofructokinases inhibitor, a thioredoxin-1 inhibitor, L- Asparaginase, a GLUT1 inhibitor, a glutaminase C inhibitor, a hexokinase inhibitor and an mTORCI and mTORC2 inhibitor. Also provided herein are compositions for inhibiting hair growth and methods of screening for inhibitors of hair growth.

The present application relates to the field of cosmetic technology, and discloses a preparation process of cashmere goat bone-derived elastin and application thereof in cosmetics. The process comprises: defatting cashmere goat bone particles; using composite rumen microbial flora to perform bionic fermentation to destruct bone matrix and expose active sites; adopting neutral protease and specific elastase to perform stepwise directional enzymolysis; performing hydrophilic modification through transglutaminase catalysis of covalent anchoring of polyglutamic acid and elastin polypeptide; and finally, performing multi-stage membrane interception purification and drying. The present application realizes complete retention of the natural cross-linking structure of elastin under mild conditions, significantly improves water solubility, biological activity and transdermal permeability of the elastin, solves the problems of structural damage and pollution caused by traditional chemical methods, and provides a green path for high-value utilization of cashmere goat by-products.

A method to prepare a mixture to make cereal-based food products which natively contain gluten includes a step of mixing cereal flour and a step of preparing a mixture obtained by mixing a hydrolyzed flour composition with a transglutaminase enzyme and with a source of lysine.

The application relates to a biomimetic hydrogel-based connective tissue and a preparation method and application thereof, and belongs to the technical field of plant-based food processing. In view of the defects of single taste and lack of real meat connective tissue feeling of existing plain meat products, a continuous phase component (containing plant protein, water-soluble dietary fiber and ferulic acid) is mixed with a pH-activated plant polysaccharide colloidal solution (containing a glucan gum, a galactomannan gum and a fucoidan gum); then, calcium ions and transglutaminase are introduced in sequence to trigger ion crosslinking and enzymatic crosslinking in sequence; and finally, a heat curing treatment and cold storage curing are carried out to obtain a biomimetic hydrogel-based connective tissue which is excellent in mechanical property, uniform in texture, high in water holding property and stable in heat. The biomimetic hydrogel-based connective tissue prepared by the application can be used for developing a new generation of high-end plant-based meat products.

Aspects of the present disclosure relate to the discovery that antagonizing pyruvate carboxylase and glutaminase inhibits cell proliferation and extracellular matrix production. Thus, methods and compositions disclosed herein may be used to treat fibrotic disease and cancer by inhibiting cell proliferation and extracellular matrix production.

Disclosed are fusion polypeptides that are substrates for white Kunitz's transglutaminase. The fusion polypeptides comprise one or more FKBP chaperone proteins and a target polypeptide. Each of these elements is separated from adjacent elements by a linker amino acid sequence. It has been found that it is advantageous to insert a glutamic acid-containing transglutaminase recognition motif into the linker amino acid chain. The subsequent labeling reaction catalyzed by the transglutaminase surprisingly provides a labeled fusion polypeptide with superior properties compared to chemically randomly labeled fusion polypeptides of similar design. Assays and kits for detecting a target antibody in a sample in vitro are provided.

The present application relates to a kind of frozen and thawed stability improving yellow croaker noodles and its preparation method. By low temperature relaxation and network stability, myofibrillar protein is fully hydrated, directional arrangement under the protection of complex antifreeze, which provides uniform, protein substrate for subsequent enzyme crosslinking, which is the structural basis for forming dense network. Then, after the secondary phase high shear, it is again placed at 0 DEG C-4 DEG C, which provides sufficient low-temperature reaction time for the generation of epsilon-gamma-glutamyl lysyl crosslinking bond catalyzed by transglutaminase (TG enzyme), realizes the covalent bond enhancement of protein network, and forms a complete primary phase grinding → low temperature relaxation → secondary phase high shear → network stability dual-phase reorganization process chain. The frozen yellow croaker noodles product provided by the present application can withstand the inevitable temperature fluctuations in cold chain transportation and home storage, and still maintain the quality of integrity, juiciness and strength after thawing, greatly extend the shelf life and consumer convenience, solve the technical pain points of traditional frozen fish noodles, which are prone to breakage, dry and taste deterioration after repeated freezing and thawing.

The application discloses a leydig's egg yolk phospholipid peptide with blood lipid-lowering and memory improving functions, and a preparation method and application thereof, and belongs to the technical field of bioactive peptide preparation. The preparation of the leydig's egg yolk phospholipid peptide with blood lipid-lowering and memory improving functions specifically comprises the following steps: mixing defatted leydig's powder and egg yolk phospholipid protein powder, adding the mixture into an enzymolysis tank, stirring uniformly, then adding bacillus subtilis protease and transglutaminase for enzymolysis, inactivating the enzyme, concentrating, and performing vacuum low-temperature spray drying to obtain the leydig's egg yolk phospholipid peptide product; the product has good dispersion stability, the raw materials are safe and non-toxic, a 'peptide-phospholipid' composite structure is constructed, activity reservation and synergistic effect are realized, and the problems of poor stability and single efficacy of leydig's peptide are solved; the product can be used for preparing functional food with blood lipid-lowering and / or memory improving functions, and is suitable for people with hyperlipidemia accompanied by cognitive function decline.

The application discloses high-digestibility yeast protein and a preparation method and application thereof. First, ordinary yeast protein powder is prepared into a yeast protein dispersion, then the protein structure and aggregation state are regulated by two-stage ultrasonic treatment under temperature control, and then glutaminase and beta-glucanase are sequentially added for enzymatic modification, thereby obtaining high-digestibility yeast protein. By the modification strategy of two-stage ultrasonic pretreatment and sequential double-enzyme treatment, the comprehensive regulation of the aggregation state, molecular conformation and polysaccharide barrier effect of the yeast protein is realized, and the overall method has good technical effect and application value. The method combines physical modification and enzymatic modification, disperses, depolymerizes and restructures the protein by two-stage ultrasonic treatment, and then adjusts the protein conformation and polysaccharide barrier by glutaminase and beta-glucanase respectively.

This invention belongs to the field of biomedical materials, and particularly relates to a hydrogel for cartilage repair and its preparation method. The preparation method of the hydrogel includes the following steps: (1) adding cross-linked modified chitosan and 8-O-acetylganoside methyl ester to a collagen solution, adjusting the pH to obtain a collagen polysaccharide solution; (2) adding metal ion microspheres to the collagen polysaccharide solution of step (1), stirring and reacting, then adding microbial transglutaminase for heating and reacting, adjusting the pH to obtain a mixed solution; (3) adding 1,4-butanediol diglycidyl ether and growth factors to the mixed solution of step (2), adjusting the pH, water bath, and dialysis to obtain the hydrogel. This preparation method is simple, and the obtained hydrogel has excellent mechanical strength and adhesion properties.

The present invention addresses the problem of providing a technique for producing a textured plant protein that is configured such that dull color formation is suppressed. Dull color formation of the textured plant protein obtained by the present invention is suppressed by adding a nontextured plant protein material that has been treated using a glutaminase and protease to a material for texturing.

This invention discloses the application of a glutamine-depleting engineered bacterium and its pharmaceutical composition. The engineered bacteria use attenuated bacteria with tumor-targeting capabilities as vector strains. The genome or plasmid of the vector strain integrates a therapeutic gene encoding glutaminase. This method achieves deep and continuous depletion of glutamine in the tumor, avoiding drug resistance caused by tumor metabolic compensation. The inhibition rate against c-Myc-overexpressing soft tissue sarcomas is significantly higher than that of small molecule inhibitors. Enzyme production is only carried out locally in the tumor, with no effect on normal tissues and no systemic toxicity. The engineered bacteria can penetrate the dense tumor matrix and colonize the tumor, solving the problem of poor tissue penetration of small molecule inhibitors. The engineered bacteria continuously multiply and produce enzymes in the tumor, achieving continuous release of glutaminase. The half-life is significantly longer than that of recombinant enzymes, eliminating the need for frequent dosing. The glutaminase optimized by protein engineering has significantly reduced immunogenicity.

This invention belongs to the field of cultured meat and relates to a method for constructing a cultured meat scaffold. The invention involves preheating a deionized aqueous solution of soybean protein and then cooling it to room temperature to obtain a soybean protein solution; dissolving fish gelatin in deionized water to obtain a fish gelatin solution; mixing the soybean protein solution and the fish gelatin solution, stirring until homogeneous to obtain a mixed solution; then adding glutaminase solution; heating and incubating to inactivate the enzyme; and after full hydration, obtaining a gel scaffold, which is then ready for consumption as a dual-protein cultured meat scaffold. Preheating and enzymatic cross-linking are used to regulate the dual-protein gel network, reducing its heterogeneity and improving its stability and strength. The dual-protein gel scaffold possesses excellent mechanical properties, a uniform network structure, and biocompatibility, which can improve the adhesion, proliferation, and spreading area of ​​porcine skeletal muscle satellite cells, while simultaneously meeting the requirements for myoblastic differentiation.

A process for preparing a liquid oat base or drink with improved soluble oat protein content includes obtaining a slurry that includes an aqueous solvent and an oat material, which includes starch and oat protein. The slurry is treated with an amylase and a protein glutaminase to obtain an amylase-protein glutaminase-treated material. The solubilized product can then be collected, thereby preparing the liquid oat base or drink.

The present disclosure provides methods of treating or preventing age-related diseases and disorders (e.g., age-related diseases or disorders associated with the glutamate pathway and / or glutaminase-1 (GLS1)) by administering to a subject in need thereof a provided compound (e.g., a compound of Formula (I)), or a pharmaceutically acceptable salt thereof, or a pharmaceutical composition thereof. The present disclosure also provides methods of modulating mitochondrial function, reducing reactive oxygen species levels, reducing pro-inflammatory signaling, and / or preventing or inhibiting cellular senescence by contacting a cell, tissue, or biological sample with or administering to a subject in need thereof a provided compound, or a pharmaceutically acceptable salt thereof, or a pharmaceutical composition thereof.

The application belongs to the field of biomedical materials, and particularly relates to a hydrogel for cartilage repair and a preparation method thereof. The preparation method of the hydrogel comprises the following steps: (1) adding cross-linking modified chitosan and 8-O-acetylmagnol methyl ester into a collagen solution, adjusting pH to prepare a collagen polysaccharide solution; (2) adding metal ion microspheres into the collagen polysaccharide solution in step (1), stirring and reacting, then adding microbial transglutaminase for heating reaction, adjusting pH to prepare a mixed solution; and (3) adding 1,4-butanediol diglycidyl ether and growth factors into the mixed solution in step (2), adjusting pH, water-bathing and dialysis to prepare the hydrogel. The preparation method is simple in process, and the obtained hydrogel has excellent mechanical strength and adhesion.

The application provides a novel glutaminase degradation agent derivative, a preparation method and application thereof, and is based on an existing KGA allosteric site inhibitor, a novel glutaminase degradation agent containing a PROTAC molecule is designed and synthesized, and glutaminase degradation and antitumor activity of the inhibitor are improved.

The technical problem of this invention lies in providing a novel protein cross-linking method. This method promotes the cross-linking reaction by allowing both oxidoreductases such as laccase and protein deamidases such as protein glutaminase to act on the substrate protein.

The present invention addresses the problem of providing: an enzyme agent containing a transglutaminase having high reactivity at a low temperature; and use of the enzyme agent. The present invention provides an enzyme agent that contains, as an active ingredient, a transglutaminase having an amino acid sequence that has a sequence identity of 90% or more with respect to the amino acid sequence of SEQ ID NO: 1.

The invention relates to a compound of formula I, wherein R1, R2, R3, R4, R5, R6, R7, R8a, R8b, R9, A and L are as defined in the specification, or a pharmaceutically acceptable salt or solvate thereof, said compound is useful in the treatment of a disease or condition which is responsive to treatment with an inhibitor of a transglutaminase.

The present invention relates to a method for manufacturing a basil tomato chicken breast bar and a basil tomato chicken breast bar manufactured by the said method, characterized by comprising the steps of: preparing a chicken breast mixture by mixing plant tissue protein hydrate, chicken breast, salt, sugar, L-sodium glutamate, isolated soy protein, wheat gluten, hemp seed meal powder, defatted peanut powder, black soybean powder, corn starch, modified starch, maltodextrin, meat flavor seasoning, ham flavor, basil tomato sauce, pea extract mixture, transglutaminase, hemp seed oil, and water; filling a casing with the prepared chicken breast mixture, heat treating, and cooling; and inserting a stick into the cooled chicken breast mixture.

The present application belongs to the field of hair dyeing post-fixing and damage repairing, discloses a kind of glycoprotein and quaternary ammonium salt-80 synergistic fixing and repairing hair care composition and its preparation method, the composition is composed of plant source glycoprotein, quaternary ammonium salt-80, transglutaminase, acryl glycoprotein conjugate and PBS buffer solution;In the preparation process, glycoprotein and quaternary ammonium salt-80 are blended and adsorbed under mild conditions, then enzymatically catalyzed crosslinking, and homogenized by ultrasonic assistance, without organic solvent, green and environmentally friendly process;Acryl glycoprotein conjugate is prepared by coupling acryloyl chloride with glycoprotein hydroxyl group, which can further crosslink under the action of light and heat;The composition uses multi-layer biological membrane and enzymatic covalent network to realize long-lasting color fixing and micro-damage repair of dyed hair.

The present invention relates to a method for the prevention, improvement, or treatment of cancer patients, particularly patients with EMT (Epithelial mesenchymal transition) subtype cancer. In cancer patients in whom the expression level of the GLS (Glutaminase) gene or the protein encoded by it is increased, when PHGDH, SHMT, and MTHFD2 inhibitors are administered in combination, 1C metabolism is more effectively inhibited, and there is a synergistic effect in inhibiting the proliferation of cancer cells in patients with intractable cancer that is difficult to treat due to recurrence, metastasis, and resistance to anticancer drugs, thereby enabling very effective treatment of cancer. Furthermore, by measuring the expression levels of the GLS gene or the protein encoded by it, information regarding personalized treatment methods can be provided to individual patients from the early stages, thereby increasing the success rate of treatment.

The present invention relates to a milk product, a UHT milk, and a dairy product obtainable form said milk product or UHT milk and methods for producing the same. The present invention relates in particular to a method for producing a UHT milk, comprising the step of (a) subjecting a raw milk to enzymatic treatment with a protein-glutaminase (PG), and further comprising the step of ultra-high-temperature (UHT) processing of the mixture obtained in step (a).

This invention provides a crocodile bone collagen peptide powder for improving bone density, its preparation method, and its application, relating to the field of biopharmaceutical technology. The preparation method includes the following steps: crocodile bone is pulverized and mixed with sodium chloride solution, followed by a first separation to obtain bone residue; water is added and mixed, followed by a second separation to obtain defatted bone residue; the defatted bone residue is mixed with water and a deodorizing agent, stirred, and separated to obtain deodorized bone residue; the deodorized bone residue is added to water, and ultrasonic-assisted extraction is performed, separating and collecting the supernatant; the supernatant is mixed with collagenase and trypsin for a first enzymatic hydrolysis; carboxypeptidase A and proline endopeptidase are added for a second enzymatic hydrolysis; the supernatant is separated, and transglutaminase is added to react, separating and obtaining the enzymatic hydrolysate; the enzymatic hydrolysate is separated using a multi-stage tangential flow membrane separation system, collecting the retentate from a 500 Da nanofiltration membrane; the final product is concentrated and dried. The crocodile bone collagen peptide powder obtained by this invention through a specific preparation method significantly improves taste, enhances stability, and strengthens the effect of improving bone density and bone calcium.