The invention discloses an
analysis method for quantitatively measuring
fumaric acid by adopting an
ultraviolet spectrophotometric method based on
copper sulfate, and belongs to the technical field of test and analysis of physical and chemical properties of materials. The method comprises the following steps: 1, preparing a plurality of
fumaric acid standard solutions with different concentrations, preparing a
copper sulfate solution, mixing the
fumaric acid standard solutions with the
copper sulfate solution, and carrying out a complex reaction; 2, performing
ultraviolet spectrophotometry after the reaction is completed, determining the maximum absorption
wavelength of the complex, and drawing a
standard curve; and 3, dissolving a to-be-detected sample in water to obtain a to-be-detected sample solution, adding a
copper sulfate solution, mixing, carrying out a complex reaction, carrying out
ultraviolet spectrophotometric determination after completion of the complex reaction to obtain
absorbance at the
wavelength of 804 nm, and substituting the
absorbance into the equation of the
standard curve to obtain the concentration of fumaric acid in the to-be-detected sample. The method has the advantages of simplicity and convenience in operation, low cost, high sensitivity and the like, and is widely applicable to quantitative analysis of fumaric acid in environmental samples, food samples, medicines and industrial products.