450 results about "Transplantation" patented technology

The invention provides an LLM command line generation tool based on OpenHarmony and a transplantation method, belongs to the technical field of industrial Internet of Things, and aims to solve the problems that a traditional TypeScript / Node. Js scheme has limitation, is insufficient in local reasoning performance, safety performance and reliability and is high in deployment cost. The tool comprises an ETS development environment module, a code migration module, a dependency library adaptation module, a security enhancement module certificate and a deployment and operation module, a zero-dependency transplantation scheme is designed for special environments of unpacked management tools such as OpenHarmony, the problem of strong coupling of a traditional Node.js application to the environment during operation is solved, and the safety of the Node. Js application is improved. And the deployment threshold of the heterogeneous system is further reduced.

The invention provides a pancreatic islet organ capable of efficiently maintaining pancreatic islet beta cell identity and a preparation method and application thereof. Specifically, the invention provides the preparation method for the in-vitro pancreatic islet organ, and the preparation method comprises the following steps: carrying out induced differentiation culture on the ZnT8-knocked-out pluripotent stem cells, so as to obtain the mature pancreatic islet organ. The invention also provides a corresponding transplantation composition and application thereof. The invention discloses that ZnT8 knockout is a key factor for effectively maintaining the identity stability of beta cells in pancreatic islet organs. In addition, the pancreas islet beta cell identity maintenance agent is combined with the ZnT8-knocked-out pancreas islet organ for use, so that the identity stability of the pancreas islet organ beta cell can be obviously maintained. The invention provides a new strategy for transplantation treatment of type 2 diabetes, and has excellent clinical transformation value.

The invention relates to the technical field of medicines, and discloses an application of a traditional Chinese medicine composition in a humanized flora transplantation model.The application comprises the following steps that an experimental body is pretreated through sterile feeding or broad-spectrum antibiotics, then coprophilous fungus suspension of a functional dyspepsia patient is transplanted, and the humanized flora transplantation model is constructed; acquiring physiological data of the humanized flora transplantation model, and performing intervention on the traditional Chinese medicine composition after the humanized flora transplantation model is completed; acquiring physiological data of the traditional Chinese medicine composition intervened humanized flora transplantation model, and analyzing an evaluation result of the traditional Chinese medicine composition intervened humanized flora transplantation model by combining the evaluation parameters of the humanized flora transplantation model. The bottleneck that the traditional animal model is greatly different from the human microflora and the curative effect and the mechanism are difficult to transform is overcome, so that the research result is closer to the clinical practice, and the molecular mechanism of the traditional Chinese medicine composition is reliably revealed.

Disclosed herein are dosage forms comprising a VAV1 degrader. More specifically, disclosed herein are dosage forms comprising 3-(2-chloro-4'-(2-oxopyridin-1(2H)-yl)-[1,1'- biphenyl]-3-yl)piperidine-2,6-dione or a pharmaceutically acceptable salt thereof. These dosage forms are useful, e.g., for treating a subject (e.g., a human subject) having a disorder or disease that can be treated by reducing the level of VAV1, for example an inflammatory or autoimmune disorder, a transplantation setting disorder, or a cancer.

The invention relates to a biomarker for screening intestinal flora transplantation donors and application of the biomarker. The biomarker comprises intestinal flora and metabolites thereof, the intestinal flora comprises phylum firmicalis, bacteroides and the like, and the metabolites comprise short-chain fatty acid, tryptophan, indole, 5-hydroxytryptamine, lipopolysaccharide and the like. According to the method, the intestinal flora of different crowds is deeply analyzed, the relationship between each related index of the intestinal flora and the intestinal flora transplantation effect is studied, sufficient clinical verification is carried out, a biomarker for screening the intestinal flora transplantation donor is excavated, and a standard for judging whether the intestinal flora transplantation donor is qualified or not is established; and a new method and a new idea are provided for efficiently and accurately screening intestinal flora transplantation donors.

The gadolinium-based cationic polymer contrast agent is synthesized by chelating a cationic polymer and gadolinium ions, has high biological safety and can be used for cartilage imaging. The prepared contrast agent adopts an articular cavity direct injection method, so that the imaging effect is improved, and the contrast dosage is reduced. When the cartilage activity is normal, the content of glycosaminoglycan in the matrix is rich, and when the cartilage is inactivated or the activity is reduced, the content of glycosaminoglycan in the matrix is obviously reduced. The gadolinium-based cationic polymer contrast agent is combined with negatively charged glycosaminoglycan in a cartilage matrix through the charge effect. The gadolinium-based cationic polymer contrast agent can be used for noninvasive and quantitative evaluation of cartilage activity in nuclear magnetic resonance imaging, especially cartilage activity and stability in transplantation.

A microfluidic 3D-printed hydrogel based on fish liver decellularized extracellular matrix for liver regeneration and a preparation method. The hydrogel is prepared by means of combining fish liver decellularized extracellular matrix (dECM) and gelatin methacryloyl (GelMA), and loading induced pluripotent stem cell-derived hepatocytes (iPSC-heps) for liver regeneration. The microfluidic 3D-printed hydrogel based on fish liver decellularized extracellular matrix exhibits excellent biocompatibility, retains intact endogenous growth factors, maintains the biological activity of cells, ensures the effective encapsulation of the cells, and facilitates the robust functional expression of the iPSC-heps. After in-vivo transplantation, the survival rate and liver function of mice with acute liver failure are significantly improved, and liver regeneration and repair are promoted.

The invention provides a software transplantation method based on a processor. The software transplantation method comprises the following steps: firstly, carrying out hardware type selection and instruction set compatibility analysis, analyzing a software binary code by utilizing a specific algorithm, counting and comparing instructions, and comprehensively evaluating hardware performance to determine adaptive hardware. Then, software transportability is evaluated, and a software dependency graph and a transplantation feasibility prediction model are constructed; then, an operating system is adapted, the loading sequence and parameters of a kernel module are dynamically adjusted, and setting of a basic input and output system is optimized; in a software transplantation implementation stage, source codes are analyzed and converted based on an abstract syntax tree, open source software adopts incremental compiling cache, binary software analyzes and processes compatibility problems, and meanwhile, a one-key installation program is optimized. And finally, performing server monomer testing and platform testing, monitoring performance, checking functions, analyzing faults and optimizing until the system meets requirements. According to the method, many key problems in the process of transplanting the software to the processor are effectively solved, and the success rate of transplanting and the stability of the system are improved.

The invention relates to application of a CSPR3 gene in promoting skeletal muscle regeneration. The invention provides a system of a transplantation material for promoting skeletal muscle injury repair and application of the system by utilizing a CSPR3 gene, and reveals that the CSRP3 gene participates in a repair process after skeletal muscle injury. The CSRP3 is knocked down in the skeletal muscle, so that the regeneration capacity of the muscle is destroyed; on the contrary, overexpression of the CSRP3 in the skeletal muscle accelerates the repair process of the skeletal muscle after injury. The skeletal muscle injury repair process can be obviously promoted through an allograft strategy, and the repair time is shortened. The invention provides a direction for promoting the development of a clinical treatment strategy for human skeletal muscle injury.

The invention relates to a flora transplantation clinical pathway standardization management method, device and equipment and a medium. The method comprises the following steps: on the basis of clinical data of a patient and screening data of a donor, executing flora suitability evaluation by using a deep learning model and selecting an adaptive donor; obtaining a flora sample of an adaptive donor, and carrying out high-throughput sequencing; generating a donor flora quality evaluation score according to a preset flora composition diversity index based on a sequencing result; screening the flora samples of which the quality evaluation scores are higher than a preset quality threshold value to prepare a flora preparation; detecting activity parameters and diversity parameters of the flora preparation, and performing colonization potential classification prediction by combining clinical data of the patient and adopting a support vector machine algorithm so as to output a potential colonization success rate; and when the potential colonization success rate exceeds a preset success rate threshold, outputting a clinical transplantation scheme through a preset transplantation scheme generation algorithm based on the flora preparation and the clinical data of the patient. According to the method, through multi-stage intelligent decision making, the safety and the effectiveness are improved.

Some embodiments relate to improved cryopreservative formulations and improved methods for cryopreserving and thawing cells. These formulations facilitate high density, low volume cell cryopreservation strategies which overcome the need for washing of cells post-thaw, and therefore result in greater cell recovery and viability. Pharmaceutical compositions comprising a cell population and a cryopreservative formulation as provided herein are also provided. Methods for treating a subject having a disease or condition that benefits from transplantation of a cryopreserved and thawed cell population are also provided.

This invention relates to the technical field of stem cell induction differentiation and transplantation therapy, specifically to a method for inducing human induced pluripotent stem cells to differentiate into dopaminergic neuronal progenitor cells, comprising the following steps: S1, human induced pluripotent stem cells are induced to differentiate into primitive neuroepithelial cells under the action of SB431542, DMH1, SHH, FGF8b, Purmorphamine, and CHIR99021; S3, intermediate primitive neuroepithelial cells are continuously induced to differentiate using BDNF, GDNF, AA, N2, B27 (without added vitamin A), cAMP, and GlutaMax to obtain dopaminergic neuronal progenitor cells. The dopaminergic neuronal progenitor cells obtained using the method provided by this invention can survive for a long time in the brain microenvironment of Parkinson's rats and have a significant repair effect on rat motor dysfunction.

Provided herein are tumor-antigen VGLL1 specific T cell receptors. The TCR may be utilized in various therapies, such as autologous cell transplantation, to treat a cancer. Methods for expanding a population of T cells that target VGLL1 are also provided.

The invention relates to a product for topical use in the treatment of skin affected by dysbiosis. The product has a pH ranging from 5.5 to 6.5 and essentially comprises water, glycerin and at least one crosslinked polyacrylate. This product can be used as a prerequisite for the transplantation of biological material favorable to the development of a healthy cutaneous bacterial flora.

The present invention relates to recellularization of xenogeneic tissue. Specifically, the present invention relates to recellularization of xenogeneic tissue, which enables transplantation of non-immunogenic biological tissue with improved durability and regenerative capacity by generating xenogeneic tissue having regenerative capacity through human cell recellularization after removal of galactose-alpha-1,3-galactose (α-Gal) xenoantigens and non-galactose (non-Gal) xenoantigens.

Featured here are methods for improving tissue or organ preservation and utilization through mitochondrial transplantation.

The utility model discloses an automatic microorganism enrichment and adsorption device and relates to the technical field of biomedicine. The device comprises an enrichment adsorption box, the enrichment adsorption box is provided with a harmful bacterium adsorption assembly used for removing harmful bacteria in intestinal microorganism mixed liquid, a beneficial bacterium enrichment assembly used for enriching beneficial bacteria in the microorganism mixed liquid, a beneficial bacterium dissociation assembly used for dissociating the beneficial bacteria and antibody magnetic beads which are coupled together, and a beneficial bacterium collection bottle used for collecting the beneficial bacteria. And the negative pressure assembly is used for enabling the intestinal microorganism mixed liquid to flow through the harmful bacterium adsorption assembly, the beneficial bacterium enrichment assembly and the beneficial bacterium dissociation assembly and enter the beneficial bacterium collection bottle. According to the utility model, specific intestinal microorganisms can be selectively screened, so that a solid foundation is laid for precise transplantation of intestinal flora and precise treatment of specific related diseases of the intestinal tract.

The invention relates to the technical field of livestock and poultry breeding, in particular to a goat hybridization breeding method capable of achieving complete-cycle nutrition enhancement. The core of the method is that systematic nutrition intervention runs through the whole breeding process. Before hybridization, performing differential nutrition pretreatment on the parents for 60 days: feeding the female parents with a breeding nutrition enhancer added with components such as D-chiro-inositol and N-acetylcysteine, and supplementing sperm motility enhancer containing acetylated L-carnitine and ergothioneine to the male parents. In the gamete treatment stage, the oocytes are subjected to in-vitro maturation culture in a special culture solution containing components such as D-chiro-inositol and a growth differentiation factor 9; after the sperms are subjected to gradient centrifugal screening, completing in-vitro fertilization in an optimized fertilization culture solution; after the embryos develop into blastocysts in the sequential culture system, carrying out laparoscopic transplantation, and carrying out staged precise nutrition culture on the pregnant ewes. According to the method, through multi-level nutrition enhancement, the reproductive capacity of the goats and the growth performance of offspring are effectively improved.

This invention discloses a device and construction method for the overall transplantation of ancient trees, belonging to the field of protective transplantation technology for ancient and famous trees. The device consists of four modular components: a main support frame, overall root system wrapping and reinforcement, flexible trunk protection support, and balanced hoisting and horizontal movement. It is detachable and reusable. Construction includes six steps: pre-planting root pruning and maintenance, root ball wrapping and reinforcement, trunk protection installation, overall lifting and horizontal movement, hoisting and transportation, and precise planting. This invention effectively avoids root ball cracking and trunk damage, is suitable for construction in confined spaces, increases the survival rate of transplanted ancient trees to over 90%, significantly shortens the recovery period, and meets the stringent requirements for protective transplantation of ancient trees.

The invention relates to the technical field of medical instruments, and discloses a disposable simple in-vitro transplantation renal artery perfusion apparatus which comprises a buckle seat, a panel and a perfusion tube, a perfusion tube mounting hole is formed in the middle of the panel, the perfusion tube is connected in the perfusion tube mounting hole in the middle of the panel in a penetrating mode, one end of the perfusion tube is connected with a perfusion tube, and the other end of the perfusion tube is connected with a drainage tube. The other end is inserted into the abdominal aorta; the buckle base comprises a bottom plate and side plates arranged at the two ends of the two sides of the bottom plate, a penetrating hole is formed in the middle of the bottom plate, and clamping grooves are formed in the inner sides of the side plates. The disposable simple in-vitro transplantation renal artery perfusion apparatus can be effectively fixed at an opening of an artery, perfusion can be carried out by connecting an infusion tube, assistance of other people is not needed, fixation is good, perfusate cannot leak out to cause waste, and intima of the artery cannot be damaged to cause series complications.

A mitochondrial transplantation system includes a mitochondrial and an apoptosis vesicular membrane, the mitochondrial being carried within the apoptosis vesicular membrane. When a system composed of mitochondria and apoptosis vesicles acts on endothelial cells, the targeting and efficiency of mitochondria transplantation are improved, mitochondria autophagy is generated, mitochondria autophagy is reactivated to remove damaged mitochondria of the endothelial cells, the removal of damaged mitochondria is promoted, and the cell functions of the damaged endothelial cells of mitochondria are recovered. The wound healing is favorably promoted. A system composed of mitochondria and apoptosis vesicle membrane is used as an active component to prepare a drug or a medical device for promoting wound healing.

The invention discloses an open type damage-free microneedle hair follicle planting pen which comprises a handle cylinder, a slidable needle withdrawing pushing assembly, a microneedle fixing assembly, a microneedle and a depth setting part. The depth setting piece is installed on the needle withdrawing pushing assembly, and a groove is formed in the end, close to the needle tip end of the micro needle, of the depth setting piece. The groove is formed in the depth setting piece, so that an operator can obtain a wide view through the groove, and hair follicles can be conveniently inserted into the side holes of the micro-needle and placed on the ejector pin. And the ejector pin can retreat into the groove, so that the effective piercing length of the microneedle is accurately shortened, and stable and uniform planting depth control is realized. And meanwhile, the contact surface of the depth setting piece and the scalp is changed from an annular shape into a local support shape by the groove structure, so that scraping and compression on surrounding implanted hair follicles are remarkably reduced in the piercing and withdrawing processes, and secondary injury is effectively avoided. The implanting pen has the functions of precise depth control, wide visual field and no-damage protection, and the survival rate, the attractiveness and the operation efficiency of hair follicle transplantation can be improved.

To provide a method for evaluating the quality of neuroretina for transplantation and neuroretina sheets for transplantation selected by said method. [Solution] The present invention provides a method for evaluating the quality of neuroretina for transplantation, comprising: extracting a part or all of a cell aggregate containing a neuroretina having an epithelial structure derived from pluripotent stem cells as a quality evaluation sample; detecting the expression of neuroretinal cell-related genes and non-neuroretinal cell-related genes in the quality evaluation sample; and determining, if the expression of neuroretinal cell-related genes is observed and the expression of non-neuroretinal cell-related genes is not observed, that (1) a neuroretina (neuroretina for transplantation) from the same cell aggregate as the cell aggregate containing a part of the quality evaluation sample, (2) a neuroretina (neuroretina for transplantation) from the same lot as the cell aggregate containing a part of the quality evaluation sample, or (3) a neuroretina (neuroretina for transplantation) from the same lot as the cell aggregate of the entire quality evaluation sample, wherein the non-neuroretinal cell-related genes include one or more genes selected from the group consisting of brain and spinal cord tissue marker genes and eyeball-related tissue marker genes.

The invention relates to the technical field of microsphere manufacturing, in particular to a device and method for preparing hydrogel microspheres wrapping organoid and application, by changing the shape and spatial distribution of a high-voltage electrostatic field, the size of the hydrogel microspheres is controlled through a voltage single variable, the preparation efficiency of the hydrogel microspheres is improved, and the hydrogel microspheres are prepared. The problems that the hydrogel microspheres prepared by a traditional high-voltage static non-contact device are large in surface roughness and the sodium alginate hydrogel microspheres are prone to fibrosis during in-vivo transplantation are solved.

The present invention provides muscle-derived progenitor cells that show long-term survival following transplantation into body tissues and which can augment non-soft tissue following introduction (e.g. via injection, transplantation, or implantation) into a site of non-soft tissue (e.g. bone) when combined with a biocompatible matrix, preferably SIS. The invention further provides methods of using compositions comprising muscle-derived progenitor cells with a biocompatible matrix for the augmentation and bulking of mammalian, including human, bone tissues in the treatment of various functional conditions, including osteoporosis, Paget's Disease, osteogenesis imperfecta, bone fracture, osteomalacia, decrease in bone trabecular strength, decrease in bone cortical strength and decrease in bone density with old age.

The utility model discloses a kind of transplanting devices for forestry trees, including fixed frame, the surface of the fixed frame is provided with movable slot, the top inner wall middle part of the movable slot is provided with first hydraulic cylinder, the piston rod bottom of the first hydraulic cylinder is provided with the lifting plate that is slidably connected with movable slot up and down, the surface upper middle part of the lifting plate is rotatably connected with second hydraulic cylinder by pivot, the surface lower side of the lifting plate is provided with lug, two the lug is rotatably connected with support frame by pivot between it.The utility model is started motor, so that blade rotates with the axis of first connecting shaft and second connecting shaft as center, so that blade rotates in soil, i. e. the root of tree and soil can be dug out, and the bottom of excavated tree is hemispherical, so excavated tree is not only convenient to transport, and main root is retained, survival rate of transplantation is greatly increased, and it is simple and convenient to operate.

This invention relates to the application of mitochondrial extracts in reversing drug resistance in solid tumors. By combining mitochondrial extracts with chemotherapeutic drugs, the resistance of chemotherapeutic-resistant solid tumors to these drugs can be reversed. Compared to using mitochondrial extracts and chemotherapeutic drugs alone, this method effectively enhances drug efficacy, thereby overcoming chemotherapeutic resistance in solid tumors. This invention, through intervention with mitochondrial extracts in different chemotherapeutic-resistant solid tumor cell lines, has verified that the combined use of mitochondrial transplantation and chemotherapeutic drugs can reverse chemotherapeutic resistance, thereby improving the efficacy of chemotherapy and opening up the possibility of continued treatment for patients with advanced solid tumors who are resistant to chemotherapeutic drugs.

Disclosed herein are enteroids, obtained by dissociating an epithelial cell population from a non-transplanted human intestinal organoid (HIO), such as an HIO produced in vitro from human pluripotent stem cells (hPSCs). Also disclosed herein are methods of producing and using the same, including methods of transplantation involving said enteroids, particularly for treating a gastrointestinal disease or condition. Also disclosed herein are methods of transplantation involving HIOs and / or HIO components, particularly for treating a gastrointestinal disease or condition.

The present invention relates to a method for producing cartilage tissue by selecting and choosing from chondrocytes or spheroids, and use thereof as pharmaceutical composition, medicinal product or transplant. In particular, the invention relates to markers for identifying suitable chondrocytes for producing cartilage tissue, in particular for the purpose of transplantation.

The invention provides a high-stress-tolerance pancreas islet organ as well as a preparation method and application thereof. Specifically, the invention provides a method for preparing pancreas islet organs. The invention also provides a use of a reagent combination comprising (a) a pancreatic islet organ and (b) a compound of formula I or a pharmaceutically acceptable salt thereof. In the presence of the compound shown in the formula I or the pharmaceutically acceptable salt thereof, pancreatic endocrine progenitor cells are cultured, so that the increase of the differentiation proportion of pancreatic beta cells is promoted, and pancreatic islet organs with high stress tolerance are obtained; according to the present invention, the combination of the pancreatic islet organ and the compound represented by the formula I or the pharmaceutically acceptable salt thereof can significantly improve and alleviate the integration stress reaction generated during the pancreatic islet organ transplantation compared to the only transplantation treatment, such that the pancreatic islet beta cell identity loss is resisted, and the stress tolerance of the pancreatic islet organ is significantly improved; therefore, the invention provides a new strategy for the treatment of diabetes, and has a wide application prospect.