979 results about "Transplantation" patented technology

The invention provides an IBS micro-ecological transplantation intelligent prediction method and system based on multi-omics driving, and relates to the technical field of biomedicine. The method comprises the following steps: establishing a multi-omics data fusion subsystem to collect metagenome, metabolome, host genome and clinical phenotype group data of a target patient; inputting the data into a flora-metabolite combined network analysis model to construct an interaction network and extracting features; generating an incidence matrix based on the features and the host genome data and calculating indexes; generating indexes through a dynamic response algorithm in combination with the clinical phenotypic data and the indexes; and outputting a curative effect prediction result by using a transfer learning framework combined with modeling. The system comprises a data acquisition module, a network analysis module, a correlation calculation module, a dynamic response module and a joint modeling module. According to the method, multiple omics data are integrated, the flora and host relation is accurately mined, intelligent prediction of the micro-ecological transplantation curative effect is achieved, powerful support is provided for IBS personalized treatment, and meanwhile data processing and safety guarantee measures are taken.

The invention discloses a leak-free core-shell hydrogel microsphere delivery system for non-contact flora transplantation as well as a construction method and application of the leak-free core-shell hydrogel microsphere delivery system. According to the invention, bacteria are encapsulated in a core of a core-shell microsphere through a two-step photo-initiation emulsion polymerization method of methacrylic acid gelatin, and a non-contact microbiota transplantation system (NMTS) is constructed. The NMTS can be used for biological encapsulation of various strains, shows encapsulation efficiency close to 100%, and has little influence on bacterial activity at the same time. NMTS demonstrates: a shielding effect that protects sealed bacteria from low pH values in the gastrointestinal tract and bile acid disruption; sufficient mechanical strength ensures that bacterial leakage is prevented and the infection risk is eliminated in the whole gastrointestinal tract delivery process; and internal and external substance exchange is realized, and the intestinal microorganism structure is remodeled. The invention provides a unique bacterial cell microencapsulation method which is a non-contact, safe and effective micropopulation transplantation method.

The invention belongs to the technical field of intestinal flora transplantation, and discloses an intestinal flora enrichment microsphere as well as a preparation method and application thereof and an enrichment method of intestinal flora. The intestinal flora enrichment microsphere provided by the invention specifically comprises a calcium alginate gel substrate and a porous adsorbent doped on the calcium alginate gel substrate, and the intestinal flora-enriched microspheres have a special pore size distribution situation, namely the pore size is gradually increased along the direction from the center to the outer layer. When the intestinal flora enrichment microspheres are applied to enrichment of intestinal flora, active flora cells can be effectively separated from small impurities such as intestinal contents and flora metabolites while the proportion of the high-activity flora cells is kept, and the intestinal flora enrichment microspheres have an excellent intestinal flora enrichment effect.

The present invention relates to the field of Chinese medicine cultivation, and in particular to a cultivation device for Chinese medicine planting with automatic temperature control, including a cultivation box, a placement plate, a cultivation frame and an electric push rod; the front and rear sides of the cultivation box are both provided with box doors; the front and rear sides of the cultivation box are respectively connected with a plurality of placement plates; each placement plate is respectively fixed with a plurality of cultivation frames; the front and rear sides of the cultivation box are respectively fixed with a plurality of electric push rods, and each two corresponding telescopic parts of the electric push rods are respectively fixed with a placement plate. The separation plate is rotated to scrape the inner wall of the cultivation frame, and the soil adhered to the inner wall of the cultivation frame is scraped off and separated, so as to facilitate subsequent transplantation, and avoid part of the soil remaining and adhering to the inner wall of the cultivation container, which cannot be fully removed, resulting in the root system of the seedling being pulled by the soil when being removed and damaged, thereby affecting the survival rate of subsequent transplantation.

The invention relates to the technical field of power transmission line monitoring, and discloses a power transmission line real-time monitoring method and platform. The method comprises the following steps: establishing a multi-omics data fusion subsystem to collect metagenome, metabolome, host genome and clinical phenotype group data of a target patient; inputting the data into a flora-metabolite combined network analysis model to construct an interaction network and extracting features; generating an incidence matrix based on the features and the host genome data and calculating indexes; generating indexes through a dynamic response algorithm in combination with the clinical phenotypic data and the indexes; and outputting a curative effect prediction result by using a transfer learning framework combined with modeling. The system comprises a data acquisition module, a network analysis module, a correlation calculation module, a dynamic response module and a joint modeling module. According to the method, multiple omics data are integrated, the flora and host relation is accurately mined, intelligent prediction of the micro-ecological transplantation curative effect is achieved, powerful support is provided for IBS personalized treatment, and meanwhile data processing and safety guarantee measures are taken.

The invention discloses a pharmaceutical composition for reversing pancreatic cancer gemcitabine drug resistance and application of the pharmaceutical composition, and belongs to the technical field of biological medicine. The composition comprises a generic FGFR inhibitor delatinib and gemcitabine, the concentration of the delatinib is 25 to 50 mg / kg, and the concentration of the gemcitabine is 20 to 30 mg / kg. By inhibiting an FGFR2 / FGFR3 signal channel and reducing the expression of FGFR protein in drug-resistant cells, the chemosensitivity of gemcitabine is synergistically enhanced, the apoptosis induction rate is increased by more than two times, and the synergic index (CI) is less than 1. In-vitro and nude mouse transplantation tumor experiments prove that the composition can significantly inhibit the proliferation of gemcitabine drug-resistant pancreatic cancer cells. The Gemcitabine drug-resistant pancreatic cancer drug can be used for preparing a drug for treating Gemcitabine drug-resistant pancreatic cancer with high FGFR3 expression, patients are screened through immunohistochemistry or gene sequencing, intravenous injection or oral administration is adopted, dosage forms comprise freeze-dried powder injection, capsules or tablets, and a new strategy is provided for pancreatic cancer drug-resistant treatment.

The discovery and application of immune checkpoint inhibitors (ICI) have significantly promoted cancer treatment. However, the response rate is still low in gastrointestinal (GI) cancers. To explore novel adjuvant treatment options, we performed fecal microbiota transplantation (FMT) of healthy donors on 10 GI cancer patients with resistance to anti-PD-(L) 1 treatment. The combination of FMT and anti-PD1 treatment was well tolerated and no serious adverse reaction was observed. Two out of 10 patients restored clinical responsiveness to anti-PD1 after FMT. Clinical benefits were shown to be associated with better colonization of co-occurred immunogenic microbes from donors, alongside activated immune status reflected by the peripheral immune cell population. Moreover, we identified microbial signatures of ICI responsiveness and validated in an independent cohort. Our study demonstrates the feasibility of FMT as a potential adjuvant therapy for ICI irresponsive GI cancer patients and laid a foundation for LBP / consortia development to enhance ICI efficacy.

The invention provides a hair follicle transplantation method and system, a program unit, a storage medium and a program product, and the method comprises the steps: enabling a tensioner to apply downward pressure to the skin, driving a first tensioning part and a second tensioning part to be away from each other, and expanding the skin to form an expanded skin region; the system comprises a mechanical arm tail end tool, the mechanical arm tail end tool moves, gets close to an expanded skin area and executes a hair follicle transplantation task, the system further comprises a controller, the controller evaluates hair follicle transplantation parameters and formulates a hair follicle transplantation plan, and in a hair follicle transplantation mode, the controller controls the mechanical arm tail end tool to perform incision at a transplantation part in a manner that the incision direction and / or depth can be controlled. And the blocking device keeps the hair follicle at the required depth, and the mechanical arm tail end tool is retracted to complete the transplantation task. According to the application, the tensioner can be automatically controlled to tension the scalp or loosen the scalp, the tensioning degree of the scalp can be adjusted, the needling depth, the needling angle and the needling density are adjusted according to the obtained tensioning degree of the scalp, the batch consistency of extracted hair follicles is improved, and the hair follicle transplantation effect is improved.

The invention discloses a bare seedling belt film ironing mechanism for mechanical transplanting and a film ironing method, the film ironing mechanism comprises an ironing knife mechanism and a bare seedling jig, and the ironing knife mechanism comprises a seat body and a film ironing unit fixed on the seat body; the bare seedling jig is provided with a base plate matched with the hot film unit; the bare seedling jig further comprises an upper seedling clamp and a lower seedling clamp which are arranged at the two ends of the base plate respectively. The partition plate is connected with the seat body, and a gap is formed between the partition plate and the seat body; the partition plate is provided with a notch which is opened downwards, and the notch is trumpet-shaped; the multiple film ironing units are columnar, the multiple film ironing units are divided into two sets to be arranged on the two sides of the partition plate respectively, and each set of film ironing units is arranged along a line parallel to the side edge of the notch. The mechanism and the method provided by the invention fully adapt to the characteristics of heat sealing of bare seedlings, and the formed heat points not only can fully restrain the bare seedlings, but also can conveniently separate the upper film from the lower film in subsequent use to release the bare seedlings.

Described herein are compounds useful in the treatment of TYK2 mediated diseases. In some embodiments, the TYK2-mediated disease is an autoimmune disease, an inflammatory disease, a proliferative disease, an endocrine disease, a neurological disease, or a disease associated with transplantation.

The invention belongs to the field of sponge forceps, and discloses a pair of cardiopulmonary transplantation hemostatic sponge forceps, which comprises a clamping part, a hemostatic component and an adjusting component, the annular frames are symmetrically arranged on the clamping surfaces of the two clamp arms; the hemostasis assembly comprises a plurality of pressing cylinders evenly distributed in the circumferential direction of the annular frame, a pull wire linked with the multiple pressing cylinders and hemostasis layers arranged on the outer sides of the pressing cylinders. Wherein the pressing cylinders are arranged to have convergent motion freedom degrees towards the central axis of the annular frame, when the adjusting knob is operated to tighten the traction line, all the pressing cylinders synchronously move inwards in the radial direction to form a dynamic contraction ring structure, the hemostasis layer forms a continuous pressure applying face matched with the outer wall of a blood vessel, and through radial sliding fit of the pressing cylinders which are evenly distributed in the circumferential direction and the annular frame, the continuous pressure applying face is formed. A contraction ring structure with the diameter capable of being dynamically adjusted is formed by combining a synchronous contraction mechanism of a traction line, and the stress uniformity of a suture line is improved by applying multiple circumferential pressures to the vascular suture line, so that local high-pressure points caused by a traditional rigid forceps holder can be weakened.

The invention provides a tissue culture method for inducing cluster buds of paeonia delavayi embryo, and relates to the technical field of tissue culture of paeonia delavayi. The tissue culture method mainly comprises the steps of explant acquisition, embryo germination culture, embryo cluster bud induction culture, cluster bud multiplication culture, cluster bud rooting culture, acclimatization and transplantation and the like. According to the method, the defects in the prior art are overcome, the embryos in fresh and mature Paeonia delavayi seeds in the current year are used as explants, a regeneration system of an embryo cluster bud induction way is preliminarily established through embryo cluster bud induction, proliferation and rooting culture medium research, and a foundation is laid for establishment of a Paeonia delavayi genetic transformation system.

The invention discloses a liver cancer image lightweight segmentation method based on UNet, and relates to the technical field of liver cancer segmentation of medical images. According to the method, a lightweight dilated convolution attention module combining local separable dilated convolution and a context anchor attention module is provided, and the feature expression ability is enhanced while the calculated amount is reduced; a self-adaptive wavelet pooling down-sampling module combining wavelet transform and a self-adaptive attention mechanism can perform further feature concentration and extraction on the feature map; a lightweight feature up-sampling and enhancement fusion module composed of a local separable cavity convolution module and a multi-frequency channel attention module can effectively improve the cross-scale feature fusion capability of the model, and maintain a low parameter amount and a low calculation amount. According to the method, while the parameter quantity and the calculation cost are remarkably reduced, the anatomical features of the liver can still be accurately captured, a finer segmentation result is provided, doctors are assisted in optimizing treatment schemes such as tumor excision and living body transplantation, and the clinical decision-making efficiency is improved.

The invention provides an LLM command line generation tool based on OpenHarmony and a transplantation method, belongs to the technical field of industrial Internet of Things, and aims to solve the problems that a traditional TypeScript / Node. Js scheme has limitation, is insufficient in local reasoning performance, safety performance and reliability and is high in deployment cost. The tool comprises an ETS development environment module, a code migration module, a dependency library adaptation module, a security enhancement module certificate and a deployment and operation module, a zero-dependency transplantation scheme is designed for special environments of unpacked management tools such as OpenHarmony, the problem of strong coupling of a traditional Node.js application to the environment during operation is solved, and the safety of the Node. Js application is improved. And the deployment threshold of the heterogeneous system is further reduced.

The embodiment of the invention provides a driver cross-platform transplantation method and device, a storage medium and electronic device.The method comprises the steps that a set of first source driving blocks and a set of second source driving blocks corresponding to source driver codes of a source platform are obtained; the source driving program code is divided into a plurality of source driving blocks, a group of first source driving blocks are code blocks which do not allow direct cross-platform transplantation, and a group of second source driving blocks are code blocks which allow direct cross-platform transplantation; obtaining a transplantation rule table of each first source driving block in the group of first source driving blocks, and modifying each first source driving block according to the transplantation rule table of each first source driving block to obtain a group of modified first source driving blocks; and based on the modified group of first source driving blocks and the modified group of second source driving blocks, generating a target driving program code, and through the method and the device, the problem of low development efficiency of a computer driving program caused by manual transplantation in related technologies is solved.

The invention discloses an intelligent hair follicle planting area measuring and calculating system based on microneedle densification, and relates to the technical field of scalp image processing. The intelligent hair follicle planting area measuring and calculating system based on microneedle encryption comprises a hair follicle center point registration module; an alopecia area measuring and calculating module; and a planting area measuring and calculating module. According to the method, center point mapping is carried out through the obtained scalp image of the target object, registration error analysis is carried out on the center point mapping process to judge whether center point positioning calibration and alopecia area measurement are carried out or not, then error analysis is carried out on the measurement process of the alopecia area, and the alopecia area measurement accuracy is improved. The method comprises the following steps of: firstly, determining whether to perform smooth intensity optimization and planting area measurement and calculation, and finally, performing error analysis on a measurement and calculation process of the planting area to determine whether to perform inter-frame displacement vector optimization, thereby being beneficial to ensuring the accuracy of hair follicle planting measurement and calculation in each link and improving the accuracy of hair follicle planting measurement and calculation. Therefore, the problem of deviation of the registration point during the measurement and calculation of the planting area in the micro-needle densification hair follicle planting process in the prior art is effectively solved.

The present invention belongs to the technical field of intestinal flora transplantation, and discloses an intestinal flora preparation, preparation method and application, and a method for detecting spore content. In the preparation method provided by the present invention, a human bacterial suspension obtained by purifying a fecal sample is treated by combining a slow heating pretreatment, a high temperature incubation treatment with a volatile ethanol solution, and a density gradient centrifugation method, which can effectively increase the spore yield and the germination rate of the obtained spores, while effectively removing harmful metabolites, viruses and other substances, thereby obtaining an intestinal flora preparation with high purity and high activity.

The invention discloses a strawberry seedling and tissue culture and non-test tube rooting combined rapid propagation method, which comprises the following steps: selecting an explant, and disinfecting; inoculating and culturing; performing non-test-tube rooting culture; and hardening seedlings and transplanting. Through non-test-tube rooting culture, the process is simplified, the pollution risk is reduced, the transplanting difficulty of tissue culture seedlings is reduced, and the survival rate is increased; through the combination of tissue culture and a non-test-tube rooting technology, the survival rate of acclimatization and transplantation is as high as 91% or above; the method realizes efficient and low-cost propagation of strawberries, and is suitable for large-scale popularization and application.

The invention relates to the technical field of biomedical materials, in particular to a mitochondrial transplantation treatment system and application thereof. The mitochondrial transplantation treatment system provided by the invention comprises mitochondria obtained by separation and a transfer vector. Wherein the mitochondria transplantation treatment system is obtained by assembling the mitochondria and the transfer vector, and the transfer vector comprises at least one of artificial lipid vesicles, vesicles derived from cell membranes and exosomes. The preparation process of the mitochondrial transplantation treatment system is simple, healthy mitochondria can be efficiently and rapidly transferred into cells or tissues at the same time, and the mitochondrial function of damaged mitochondrial cells is repaired. The mitochondrial transplantation treatment system has the potential of treating related diseases caused by mitochondrial dysfunction.

The invention provides a tissue culture method and an acclimatization and transplantation method of coptis chinensis, and belongs to the technical field of plant tissue culture. The tissue culture method of the coptis chinensis comprises the following steps: (1) taking a tender shoot of a coptis chinensis rhizome as an explant, sterilizing, and inoculating into a basic culture medium for culturing, so as to obtain a coptis chinensis sterile seedling; (2) inoculating the aseptic seedlings of coptis chinensis into an adventitious bud differentiation culture medium for culture to obtain adventitious buds of coptis chinensis; (3) inoculating the coptis chinensis adventitious buds into a proliferation culture medium for culturing to obtain coptis chinensis proliferation seedlings; and (4) inoculating the coptis chinensis multiplication seedlings into a rooting culture medium for culture to obtain coptis chinensis test-tube seedlings. The tissue culture and acclimatization and transplantation method provided by the invention can improve the propagation efficiency of the coptis chinensis, remarkably shorten the growth cycle of the coptis chinensis, provide technical support for rapid propagation and sustainable utilization of resources of the coptis chinensis, and have wide application prospects.

The invention discloses markers for diagnosing and treating tumors. It is found for the first time that overexpressed circRNABP9 can inhibit proliferation, migration and invasion of gastric cancer cells and promote aging of the gastric cancer cells; the circTMEM45A can inhibit proliferation, migration and invasion of esophageal cancer cells, and further through mouse transplantation tumor experiment verification, a new thought and direction are provided for diagnosis and treatment of gastric cancer and esophageal cancer, and the circTMEM45A has a wide application prospect.

The invention provides a pancreatic islet organ capable of efficiently maintaining pancreatic islet beta cell identity and a preparation method and application thereof. Specifically, the invention provides the preparation method for the in-vitro pancreatic islet organ, and the preparation method comprises the following steps: carrying out induced differentiation culture on the ZnT8-knocked-out pluripotent stem cells, so as to obtain the mature pancreatic islet organ. The invention also provides a corresponding transplantation composition and application thereof. The invention discloses that ZnT8 knockout is a key factor for effectively maintaining the identity stability of beta cells in pancreatic islet organs. In addition, the pancreas islet beta cell identity maintenance agent is combined with the ZnT8-knocked-out pancreas islet organ for use, so that the identity stability of the pancreas islet organ beta cell can be obviously maintained. The invention provides a new strategy for transplantation treatment of type 2 diabetes, and has excellent clinical transformation value.

The invention discloses an application of a small molecular compound LZ-A4 in preparation of a preparation or a medicine for treating malignant tumors. The chemical formula of the small molecule compound LZ-A4 is C19H11Cl2N3O3S, and the small molecule compound LZ-A4 can promote degradation of LGMN in malignant tumor cells, inhibit proliferation of tumor cells and inhibit polarization of macrophages to M2 type. The research on a colorectal cancer mouse transplantation tumor model proves that the development of the colorectal cancer can be effectively inhibited by single treatment of the LZ-A4, and the volume of the tumor can be further reduced after the LZ-A4 is combined with a clinical first-line chemotherapeutic drug Oxaliplatin for the colorectal cancer. Meanwhile, the LZ-A4 can inhibit M2 type polarization of macrophages in a tumor microenvironment in vivo, promote infiltration of cytotoxic T cells, remodel the tumor microenvironment and activate anti-tumor immunity. In addition, the A4 has good toxic and side effects, and does not cause further reduction of the body weight of the mouse after being combined with chemotherapeutic drugs.

An object of the present invention is to provide a cell population suitable for transplant of retinal tissue and a method of production thereof. The present invention provides a cell population for transplant, comprising retinal cells with a modified bipolar cell-regulating gene and a method of production thereof.

The invention discloses a natural product-induced artificial nano-vesicle derived from human umbilical cord mesenchymal stem cells and application of the natural product-induced artificial nano-vesicle in ischemic diseases. The preparation method of the nano vesicle derived from the human umbilical cord mesenchymal stem cells comprises the following steps: S1, inoculating the human umbilical cord mesenchymal stem cells to a 3D microcarrier; then adding traditional Chinese medicine active ingredients to activate the human umbilical cord mesenchymal stem cells, continuing to culture, cracking the microcarrier, and harvesting cells; s2, dispersing the human umbilical cord mesenchymal stem cells obtained in S1 to obtain a suspension, extruding the suspension through a polycarbonate film, and then discarding large particles by adopting a gradient centrifugation method to obtain the nano-vesicles. Compared with naturally secreted exosomes, the exosome has the advantages that the yield is increased, the treatment efficiency is improved, the production cost is reduced, and compared with stem cells, the problems of low transplantation rate and the like are avoided. The stem cells are pretreated based on the traditional Chinese medicine, the vesicles are extracted by utilizing an extrusion method, and the yield and the treatment efficiency of the nano vesicles are remarkably improved.

The utility model belongs to the technical field of asparagus planting, and particularly relates to an asparagus seedling transplanting device which comprises a transverse frame rod, rotating rods are symmetrically and rotationally arranged on the lower side of the middle of one side face of the transverse frame rod, fixing blocks are arranged on one sides of the lower ends of the rotating rods, semicircular buckets are arranged on the sides, away from the rotating rods, of the fixing blocks, and the semicircular buckets are symmetrically arranged. Side connecting blocks are arranged in the middles of the sides, away from each other, of the rotating rods, pushing rods are rotationally arranged on the sides, away from the semicircular bucket shovels, of the side connecting blocks, the upper ends of the pushing rods are close to each other and jointly rotationally provided with a rotating column in an inserted connection mode, and an inserted connection rod is arranged at the upper end of the rotating column; the inserting rods penetrate through the first sleeves to the upper sides to be provided with handle rods, the inserting rods are sleeved with springs between the first sleeves and the handle rods, and vertical rods are arranged at the two ends of the lower side of the transverse frame rod. The asparagus seedlings can be conveniently and rapidly put and transplanted, the convenience of transplanting the asparagus seedlings is improved, manpower is saved, and the efficiency is improved.

Provided are TYK2 inhibitors of Formula I, processes for their production, their use as pharmaceuticals and pharmaceutical compositions comprising them. These compounds are useful, for example, in treating TYK2-mediated disorders, such as, autoimmune and inflammatory disorders, metabolic disorders, proliferative disorders, endocrine disorders, neurological disorders, allergic disorders, and disorders associated with transplantation.

According to the engineering immune cell for immunotherapy and the preparation method thereof, the engineering immune cell is more durable in a host organism, and the transplantation survival rate is high.

The invention relates to the technical field of medicines, and discloses an application of a traditional Chinese medicine composition in a humanized flora transplantation model.The application comprises the following steps that an experimental body is pretreated through sterile feeding or broad-spectrum antibiotics, then coprophilous fungus suspension of a functional dyspepsia patient is transplanted, and the humanized flora transplantation model is constructed; acquiring physiological data of the humanized flora transplantation model, and performing intervention on the traditional Chinese medicine composition after the humanized flora transplantation model is completed; acquiring physiological data of the traditional Chinese medicine composition intervened humanized flora transplantation model, and analyzing an evaluation result of the traditional Chinese medicine composition intervened humanized flora transplantation model by combining the evaluation parameters of the humanized flora transplantation model. The bottleneck that the traditional animal model is greatly different from the human microflora and the curative effect and the mechanism are difficult to transform is overcome, so that the research result is closer to the clinical practice, and the molecular mechanism of the traditional Chinese medicine composition is reliably revealed.

The invention relates to the technical field of medical information processing, in particular to a wound condition ultrasonic assessment method for free flap transplantation, which comprises the following steps: performing block processing on an acquired ultrasonic gray image to obtain each enhancement analysis candidate block, the block processing being beneficial to improving the contrast ratio of a fat area and an edema area; a plurality of enhanced analysis target blocks are screened through fat feature screening parameters, so that the image data analysis amount is reduced to a certain extent, and the wound condition ultrasonic evaluation efficiency is improved; a blood vessel ultrasonic frequency shift feature value and a fat echo factor are combined to determine a fat feature first evaluation value of an enhanced analysis target block, and then a fat feature second evaluation value of each gray level is determined, so that enhancement of fat discrimination under the condition that a probe is in poor contact with wound skin is facilitated; and an enhanced ultrasonic grayscale image which has a better enhancement effect and is used for ultrasonic evaluation of wound injury conditions is obtained.