711results about How to "Effective treatment" patented technology

The invention discloses carboxymethyl chitosan-hyaluronic acid-stem cell exosome eye drops as well as a preparation method and application thereof, and relates to the technical field of eye drops and medical biogel. The carboxymethyl chitosan-hyaluronic acid-stem cell exosome eye drops are prepared from tannic acid modified carboxymethyl chitosan and adipose tissue-derived stromal cell exosome freeze-dried powder, the carboxymethyl chitosan-hyaluronic acid-stem cell exosome eye drops provided by the invention can effectively treat xerophthalmia, and are stable in components, safe and reliable. The three components including the tannic acid modified carboxymethyl chitosan, the hyaluronic acid and the adipose tissue-derived stromal cell exosome coexist, so that the functions of all the components can be fully played, a synergistic effect is achieved, and a better treatment effect on xerophthalmia is achieved.

The invention discloses an acupuncture robot system in the field of acupuncture equipment, and the system comprises an acupoint recognition module which is used for collecting body surface image information and sign data of a patient, and obtaining acupoint position information through a pre-trained deep learning model; the mechanical arm control module is used for controlling the mechanical arm to perform acupuncture operation according to the acupoint position information and collecting force feedback information in real time; the monitoring feedback module comprises a physiological monitoring unit and a real-time feedback unit, and the physiological monitoring unit is used for monitoring the physiological reaction of the patient and obtaining physiological information; the real-time feedback unit is used for collecting and processing the acupoint position information, the force feedback information and the physiological information so as to adjust the acupuncture strength and angle in real time. According to the acupuncture robot system, the image recognition and sensor fusion technology is introduced, the accuracy of acupoint positioning is improved, physiological data of a patient can be collected in real time, the acupuncture applying force and depth are adjusted according to the physiological data, and the treatment effect is further improved.

The invention discloses manganese and cerium co-doped modified sodium niobate, which is prepared from Nb2O5, MnO2, CeO2 and sodium hydroxide. The invention further discloses magnesium-based bone cement with the sonodynamic / nano-enzyme antibacterial function and the piezoelectric osteogenesis function. The magnesium-based bone cement is prepared from CaHPO4, NaH2PO4, MgO powder, hydroxyapatite and manganese-cerium co-doped modified sodium niobate. The magnesium-based bone cement with sonodynamic / nano-enzyme antibiosis and piezoelectric osteogenesis provided by the invention has piezoelectric enhanced sonodynamic and nano-enzyme synergistic antibacterial activity and piezoelectric osteogenesis activity, effectively removes bacteria / biological membranes, realizes combination of antibiosis and bone repair under different ultrasonic powers, effectively treats infectious bone defects, and has a good application prospect. The treatment effect of the MPC basal bone repair material is obviously improved.

The invention belongs to the technical field of stem cells, and particularly relates to a product containing mesenchymal stem cells, a preparation method, a quality control method and application in prevention and treatment of digestive system diseases. According to the preparation method, 1-3 complete culture media are provided for culturing the mesenchymal stem cells, and the obtained mesenchymal stem cells are good in subpackage form; cD73, CD90 and CD105 are highly expressed, and CD19, CD34, CD45, CD11b and HLA-DR are not expressed; the cell proliferation capacity is strong, the osteogenesis and adipogenic differentiation capacity is strong, and the medicine is safe and free of toxic and side effects. The prepared mesenchymal stem cells are high in quality, have good performance of secreting HGF, KGF and IGF-1, and can effectively treat digestive system diseases.

The invention provides a sulfoximine compound with an FGFR (Factor Growth Factor Receptor) inhibiting effect, a pharmaceutical composition containing the sulfoximine compound and application of the sulfoximine compound. The invention provides a sulfoximine compound which can be used as a fibroblast growth factor receptor (FGFR) inhibitor and is shown in a formula I, a tautomer, a stereoisomer, a hydrate, a solvate and pharmaceutically acceptable salt of the sulfoximine compound, a pharmaceutical composition containing the sulfoximine compound, and application of the sulfoximine compound in preparation of the pharmaceutical composition. Wherein R1, R2, R3, R4, R5, CyA, CyB, CyC and the like have the definitions given in the specification. # imgabs0 #

The present disclosure relates to compounds, compositions and methods for treating diseases or conditions mediated by aberrant histone deacetylase 8 (HDAC8) activity. In some embodiments, the disclosed compounds comprise an HDAC8 targeting ligand TL moiety covalently linked to a degradation determinant moiety that recruits E3 ubiquitin ligase to HDAC8. In some embodiments, the degradation determinant can bind to an E3 ligase, which is a von Hippel-Lindau (VHL) tumor suppressor, and the E3 ligase can bind to an E3 ligase, which is a Von Hippel-Lindau (VHL) tumor suppressor.

The invention discloses an artificial antigen presenting cell for efficiently preparing a chimeric antigen receptor T cell (CAR-T) and a construction method of the artificial antigen presenting cell, and belongs to the field of cell therapy and immunotherapy, the artificial antigen presenting cell takes a K562 cell as a platform, an anti-CD3 single-chain antibody, an anti-CD28 single-chain antibody, a membrane binding type cell factor and a costimulatory ligand are stably expressed through genetic engineering, and the chimeric antigen receptor T cell (CAR-T) is obtained. And the local concentration of the anti-CD3 antibody is improved by adopting an HOTag3 tool. The multi-signal integration strategy obviously enhances the activation and proliferation capacity of the T cells, promotes the CAR-T cells to maintain a low differentiation state, and endows the CAR-T cells with excellent anti-tumor activity and durability. The artificial antigen presenting cell provides a novel efficient tool for the field of cellular immunotherapy, and is expected to promote development of more efficient and safer CAR-T therapy to cope with challenges of various malignant tumors.

The invention relates to application of a GATA3 inhibitor and a PD1 inhibitor in preparation of a medicine for treating breast cancer, and belongs to the technical field of biological medicine. The invention discloses application of a GATA3 inhibitor and a PD1 inhibitor in preparation of a medicine for treating breast cancer. The GATA3 regulates the transcription regulation of IFNbeta by inhibiting the enhancer activity of IFNB1, down-regulates an I-type interferon signal channel, inhibits the secretion of an MHC-I antigen presentation channel and a chemotactic factor CCL5 of breast cancer cells, reduces immune recognition, and achieves the purpose of immune escape. The immunotherapy for inhibiting GATA3 combined with the PD1 inhibitor to treat breast cancer effectively treats breast cancer mice, improves the IFN beta level in the mice, promotes the MHC-I antigen presentation pathway of the mice, improves the CCL5 level in the mice, and improves the CD8 + T cell level in a tumor immune microenvironment, thereby reducing tumor occurrence and inhibiting tumor growth.

The invention discloses a preparation method of recombinant collagen nano-composite hydrogel for repairing wounds infected by multiple drug-resistant bacteria, which comprises the following steps: binding copper porphyrin pi-pi on titanium carbide nanosheets to form MXene coated CuTCPP nanoparticles, and carrying out amidation reaction on 3-aminophenylboronic acid and recombinant collagen to obtain the recombinant collagen nano-composite hydrogel for repairing wounds infected by multiple drug-resistant bacteria. MXene coated CuTCPP is doped into a modified recombinant collagen system and mixed with a polyvinyl alcohol solution, and the recombinant collagen nano-composite hydrogel is prepared. The self-healing property endowed by the dynamic cross-linking characteristic of the phenylboronic acid ester bond in the composite hydrogel disclosed by the invention can be highly adapted to irregular wounds; the nano particles with the heterojunction structure can be combined with photothermal therapy and photodynamic therapy to efficiently sterilize wounds; the hydrogel has the antibacterial property and the property of promoting wound healing, and can comprehensively and efficiently treat wounds infected by multiple drug-resistant bacteria.

The invention belongs to the technical field of biomedicine, and particularly relates to application of GABA (gamma-aminobutyric acid) driven by lactobacillus rhamnosus to treatment of cerebral malaria of mice. According to the invention, the obviously enriched lactobacillus muricatum is found, and the lactobacillus muricatum is proved to be capable of generating GABA (gamma-aminobutyric acid). Afterwards, on the mouse cerebral malaria model, under the condition that the lactobacillus rhamnosus and the GABA are independently used or auxiliary dihydroartemisinin is used, the effect of the lactobacillus rhamnosus and the GABA on improving and treating the mouse cerebral malaria is evaluated. Results show that the strain and the metabolite GABA thereof can effectively improve behavioristics of mice and immune pathological injuries of all organs, reduce protozoemia and prolong the lifetime of the mice whether the strain is independently used or combined with dihydroartemisinin, and can effectively treat cerebral malaria of the mice compared with the strain which is independently used with drugs. The invention provides a brand-new solution for treating severe malaria, especially human brain malaria, caused by malignant malaria from the perspective that the probiotics drive metabolites of the probiotics to assist the anti-malarial drug in treating rat brain malaria.

The invention provides a curcumin-antibacterial peptide-tetrahedral framework nucleic acid compound as well as a preparation method and application thereof, and belongs to the field of medicines. The curcumin-antibacterial peptide-tetrahedral framework nucleic acid compound is a compound prepared by taking curcumin, antibacterial peptide and tetrahedral framework nucleic acid as raw materials. Cell experiments show that the curcumin-antibacterial peptide-tetrahedral framework nucleic acid compound can significantly improve the osteogenesis activity of periodontal ligament stem cells, recover the mineralization activity, reduce the oxidative stress level and inhibit the occurrence and development of inflammatory reactions. Animal experiments show that the curcumin-antibacterial peptide-tetrahedral framework nucleic acid compound can significantly reduce loss of alveolar bones of periodontitis rats, inhibit the inflammatory state, promote osteogenic activity, recover the structure and quality of the alveolar bones and effectively treat periodontitis. The curcumin-antibacterial peptide-tetrahedral framework nucleic acid compound provided by the invention has a wide application prospect in preparation of drugs for preventing and / or treating periodontitis.

The invention discloses injectable hydrogel with anti-inflammatory and drainage functions for promoting anal fistula healing and a preparation method of the injectable hydrogel. The preparation method comprises the following steps: adding acrylic acid-N-succinimide ester and acrylated beta-cyclodextrin into a gelatin solution; then adding a carbon nanotube grafted with poly (vinyl benzyl trimethyl ammonium chloride), stirring and carrying out ultrasonic treatment; adding ammonium persulfate, stirring and performing ultrasonic treatment to obtain a mixture A; and heating the mixture A, cooling, washing and mechanically crushing to obtain the GAA coated CNT-g-PVBTMA hydrogel. The hydrogel is novel quaternized molecular brush grafted injectable hydrogel, has excellent swelling resistance and a broken porous structure, can effectively fill a fistula, and is beneficial to discharge of exudate; the hydrogel can also capture inflammatory cytokines of the fistula through electrostatic interaction, and shows excellent anti-inflammatory characteristics.

The preparation method comprises the following steps: crushing epimedium sagittatum to prepare paste, suspending with water, extracting with petroleum ether, dichloromethane, ethyl acetate and n-butyl alcohol respectively to obtain extractum of each part, carrying out gradient elution separation by using silica gel column chromatography, and concentrating to obtain the isopentenyl flavone compound extracted from epimedium sagittatum, thereby obtaining the isopentenyl flavone compound extracted from epimedium sagittatum. Combining the similar parts according to a thin layer chromatography (TLC) color development result to obtain a plurality of polar segments; carrying out chromatography and gradient elution on the target polar section to obtain a plurality of components, and then combining the similar components through thin-layer chromatography (TLC) dot plate absorption to obtain a plurality of sub-components; a target component is eluted and purified, fractions with different retention times are collected and dried, and two compounds are respectively obtained, have a remarkable inhibition effect on the cell viability of human breast cancer cells MCF-7, have anti-breast cancer activity, can be applied to preparation of drugs for treating breast cancer, and have huge social and economic benefits.

The invention discloses a traditional Chinese medicine composition for treating chronic constipation and a preparation method thereof.The traditional Chinese medicine composition is prepared from eucommia ulmoides, radix clematidis, aster, rhizoma atractylodis macrocephalae, mangnolia officinalis, poria cocos, codonopsis pilosula, immature bitter orange, cynomorium songaricum, cistanche deserticola, semen cassiae and glycyrrhiza uralensis. The traditional Chinese medicine composition has the functions of warming and tonifying liver and kidney, tonifying kidney and strengthening essence, diffusing and lowering lung qi, invigorating spleen and replenishing qi, eliminating dampness and promoting diuresis and the like, so that body fluid is generated, intestines are moistened and nourished, qi movement is smooth, large intestine conduction is recovered, and constipation, especially chronic constipation of old people or people with deficiency of qi and blood, is effectively treated.

The invention belongs to the technical field of biomedicine, and particularly relates to an iridium and chromium co-doped long afterglow nano material as well as a preparation method and application thereof. The iridium and chromium co-doped zinc gallium germanate long-afterglow nano-material is a iridium and chromium co-doped zinc gallium germanate long-afterglow nano-material, the molecular formula of the iridium and chromium co-doped zinc gallium germanate long-afterglow nano-material is Zn1. 3Ga1. 6Ge0. 1O4: Cr 0.0075, and Ir 0.05, and the invention provides a preparation method of the iridium and chromium co-doped zinc gallium germanate long-afterglow nano-material. The iridium and chromium co-doped long afterglow nano material has afterglow imaging capability, peroxidase-like activity and photothermal conversion potential, can realize cell imaging and photothermal imaging of tumors after being used, and has the potential of realizing tumor treatment. In addition, in the electro-catalytic reaction, the iridium and chromium co-doped long afterglow nano material improves the oxygen evolution reaction of the electrode under the light irradiation condition.

The invention belongs to the technical field of medicines, and relates to an acinetobacter baumannii AbaR protein binding preparation and application thereof. The morusin O can be effectively combined with AbaR protein in acinetobacter baumannii, so that an AHL quorum sensing system of the acinetobacter baumannii is inhibited. It is proved that morusin O can significantly inhibit the biofilm forming ability of the Acinetobacter baumannii ATCC 19606 strain, reduce the motility of bacteria and reduce the toxicity of bacteria to cells, and does not significantly affect the growth of bacteria to cause selective pressure to promote the evolution of drug resistance of bacteria.

The invention relates to a traditional Chinese medicine composition with functions of tonifying qi and nourishing blood for livestock. The traditional Chinese medicine composition comprises the following components in parts by weight: 10-30 parts of astragalus membranaceus, 10-40 parts of spina gleditsiae, 10-20 parts of angelica sinensis, 10-20 parts of codonopsis pilosula, 10-30 parts of uniflower swisscentaury root, 10-30 parts of ligusticum wallichii, 10-20 parts of cowherb seed, 10-20 parts of fruit of liquidambar formosana hance and 1-10 parts of iron sucrose. The traditional Chinese medicine composition is scientific and reasonable in component, free of toxic and side effects and medicine residue, convenient to take and low in cost, has the functions of tonifying qi and nourishing blood, stimulating the menstrual flow and prompting lactation, and is capable of effectively treating diseases of postpartum hypogalactia, breast milk stoppage and the like which are normal in livestock.

The invention relates to a cartilage stem cell for promoting joint repair and application thereof. In particular, the present invention provides a cellular preparation for alleviating or treating osteoarthritis. The cell line comprises Procr-positive pluripotent stem cells. The novel cartilage stem cell provided by the invention can be differentiated in vivo to generate downstream cartilage cells, and cartilage repair in osteoarthritis injury is promoted; it is prompted that selection of Procr positive cartilage stem cells for cell treatment will be a more effective treatment method. And the Procr protein can be used as a new target of the medicine for treating osteoarthritis.

The invention provides a culture method for inducing and amplifying central memory T cells with high expression of CD62L typing, which comprises the following steps: collecting peripheral blood mononuclear cells, adding the peripheral blood mononuclear cells into a culture medium containing stimulant CD3 / CD28 antibody coupling magnetic beads, amplifying for 7-12 days to obtain a cell population with TCM, and the culture medium further comprises IL-2, IL-7, IL-15 and syringaresinol. The method can effectively improve the TCM cell content and purity of the high-expression CD62L marker, and can be effectively used for treating tumors, infection or other immune related diseases.

The invention belongs to the field of biological medicine, and relates to siRNA for inhibiting PLN gene expression and a conjugate, the siRNA comprises a positive-sense strand and an antisense strand, the positive-sense strand comprises a nucleotide sequence I, and the antisense strand comprises a nucleotide sequence II; each nucleotide in the nucleotide sequence I and the nucleotide sequence II is modified or unmodified nucleotide; the nucleotide sequence I and the nucleotide sequence II are at least partially reversely complementary to form a double-stranded region; the nucleotide sequence I is basically consistent with a first section of nucleotide sequence, and the first section of nucleotide sequence is a section of nucleotide sequence with the length of at least 19 nucleotides in mRNA expressed by the PLN gene. The siRNA and the conjugate and the pharmaceutical composition thereof disclosed by the invention can effectively prevent and / or treat diseases associated with PLN expression, such as heart failure, cardiomyopathy, arrhythmia and the like, and have a good patent medicine prospect.

The embodiment of the invention discloses an intelligent follow-up visit method and device for a patient after diagnosis, a storage medium and a program product. The method comprises the following steps: creating an employee list and a patient list; determining push time based on the patient list; when the push time arrives, selecting an optimal medical worker based on the staff list and the patient list; a follow-up visit task is pushed to the terminal device of the optimal medical worker; acquiring medical data of the patient in response to a follow-up visit request of a medical worker for the patient; performing health detection on the patient based on the medical data; if abnormity occurs, early warning information is generated, treatment guidance is conducted on the patient based on the early warning information, and the follow-up visit task is pushed again. According to the method, automatic follow-up task pushing, remote follow-up and monitoring can be achieved, labor cost is reduced, follow-up work efficiency, quality and comprehensiveness are improved, more convenient medical services are provided for patients, and medical resources can be effectively utilized.

The preparation method of the isopentenyl flavone enantiomer compound comprises the following steps: crushing epimedium sagittatum to prepare paste, suspending with water, respectively extracting with petroleum ether, dichloromethane, ethyl acetate and n-butyl alcohol to obtain extractum of each part, carrying out gradient elution separation by using silica gel column chromatography, and concentrating to obtain the isopentenyl flavone enantiomer compound, namely the isopentenyl flavone enantiomer compound. Combining the similar parts according to a thin layer chromatography (TLC) color development result to obtain a plurality of polar segments; carrying out chromatography and gradient elution on the target polar section to obtain a plurality of components, and then combining the similar components through thin-layer chromatography (TLC) dot plate absorption to obtain a plurality of sub-components; a target component is eluted and purified, fractions with different retention times are collected and dried, and four compounds are obtained respectively, have a remarkable inhibition effect on the cell viability of human breast cancer cells MCF-7, have anti-breast cancer activity, can be applied to preparation of drugs for treating breast cancer, and have huge social and economic benefits.

The invention belongs to the technical field of tumor treatment, and relates to a nanometer material for treating tumors based on near-infrared light response in combination with photo-thermal, photodynamic and nitric oxide as well as preparation and application of the nanometer material. The invention relates to a near-infrared light response-based nano material for combined treatment of tumors, which is of a sphere-like structure, takes hollow mesoporous copper sulphide with photo-thermal performance as a carrier, loads a nitric oxide donor, and wraps a phase change material containing a photosensitizer. According to the nano material, photo-thermal conversion of the hollow mesoporous copper sulfide nano particles is triggered through near-infrared light, high temperature is generated, the phase change material is molten, and release of the photosensitizer IR780 and L-Arg is promoted; under irradiation of near-infrared light, IR780 generates active oxygen, and L-Arg is further triggered to release nitric oxide. The linkage mechanism of thermal controllable drug release closely combines photo-thermal, photodynamic and nitric oxide treatment, and the construction of the obtained nano material solves the problem that < 1 > O2 and NO cannot be effectively supplied to tumor sites.

The invention relates to the technical field of urinary tract infection detection, and discloses a phage-based rapid and accurate urinary tract infection detection method, which comprises the following steps: S1, constructing a CRISPR / Cas9 system; s2, constructing a homologous recombinant plasmid; s3, constructing three plasmids BL21; s4, constructing, screening, verifying and reporting a phage T4:: Nluc; according to the detection method, a CRISPR / Cas9 system is combined with a homologous recombination technology, so that the efficiency of bacteriophage gene editing is remarkably improved, based on the report bacteriophage T4:: Nluc, rapid, sensitive, specific, accurate, simple and convenient detection on clinical urinary tract infection escherichia coli is realized, and the method can be used for detecting an escherichia coli urinary tract infection sample which is not pretreated, and has a wide application prospect. The kit shows excellent detection timeliness and specificity, is a powerful tool for identifying clinical urinary tract infection escherichia coli, and provides a new technical path for timely and accurate diagnosis and effective treatment of clinical urinary tract infection.

The invention discloses a virtual reality psychological disease cognition experience method and system, and relates to the field of virtual reality psychological disease cognition experience.In actual use, cognition experience is achieved through the virtual reality technology, and the best VR scene and intervention strategy are automatically recommended according to information data of a patient through the big data analysis technology; according to the invention, automatic personalized treatment design is carried out according to the specific condition and demand of each psychological disease patient, and specific problems of the psychological disease patients are more accurately solved, so that effective treatment is provided for each psychological disease patient, and the problem that drug dependence may be caused to the psychological disease patients due to long-term medication is avoided.

The invention aims to provide a preparation method and application of a nano-enzyme hydrogel dressing, and belongs to the technical field of wound repair materials, the method prepares a nano-enzyme Mn-ZIF (at) GOx which is composed of manganese and zinc doped MOF and has multi-enzyme activity, and the nano-enzyme Mn-ZIF (at) GOx is anchored on bacterial cellulose hydrogel. Mn-ZIF (at) GOx / BC shows an antibacterial property independent of ROS (reactive oxygen species) and a relatively strong immunoregulation characteristic. According to the hydrogel, by simulating the cascade process of superoxide dismutase and catalase, hydroxyl free radicals, superoxide anions and hydrogen peroxide are effectively removed, O2 is generated, and M2 polarization of macrophages can be induced, so that inflammation is relieved. MZGB creates a good immune microenvironment through reprogramming macrophages, and proliferation, migration and angiogenesis of fibroblasts and endothelial cells are remarkably promoted through a paracrine mechanism. The MZGB hydrogel disclosed by the invention has the characteristics of resisting inflammation, removing active oxygen, resisting bacteria and promoting angiogenesis, obviously enhances the healing of diabetic wounds, and is simple to prepare.

The invention provides a small nucleic acid composition, application of the small nucleic acid composition in preparation of a medicine and a product, and belongs to the technical field of biological medicine. The invention designs a small nucleic acid composition ASO133 / 7 / 142 / 30 containing four corresponding antisense oligonucleotide fragments aiming at four regions of miR-133, let-7, miR-142-3p and miR-30 seed sequences contained in a 3 '-terminal untranslated region sequence of ATP2A2 mRNA (messenger ribonucleic acid), and the small nucleic acid composition ASO133 / 7 / 142 / 30 has a nucleotide sequence as shown in SEQ ID NO. 1. By adopting the small nucleic acid composition provided by the invention, the expression level of ATP2A2 in HL-1 cells can be increased, and the expression level of ATP2A2 in heart tissues can be remarkably improved.

The invention relates to a construction method and application of a recombinant oncolytic virus VSV-sPD1IL15RaIL15. The recombinant oncolytic virus VSV-sPD1IL15RaIL15 is constructed by using homologous recombination, and the recombinant oncolytic virus VSV-sPD1IL15RaIL15 can be used for preparing the recombinant oncolytic virus. The preparation method comprises the following steps: firstly designing sPD1 and IL15RaIL15, then fusing the sPD1 and the IL15RaIL15, synthesizing a sPD1IL15RaIL15 gene, amplifying the sPD1IL15RaIL15 gene, inserting the sPD1IL15RaIL15 gene into an ATU region of a VSV whole genome cDNA cloning plasmid pT7-VSV by using a homologous recombination method to obtain a virus whole genome plasmid pT7-VSV-sPD1IL15RaIL15, and co-transfecting the virus whole genome plasmid pT7-VSV-sPD1IL15RaIL15 and an auxiliary plasmid to a 293T cell to prepare the VSV-sPD1IL15RaIL15 oncolytic virus. According to the oncolytic virus, specific anti-tumor immunity is activated, and sPD1IL15RaIL15 is locally expressed in a tumor, so that a long-acting anti-tumor immune response is generated. When the oncolytic virus is used for treating solid tumors, the functions of tumor specific immune cells in a tumor microenvironment can be improved, infiltration of CD8 + T cells and NK cells can be promoted, and the lifetime of tumor-bearing mice can be remarkably prolonged.

The invention discloses a preparation method and application of a porous GelMA microneedle for delivering AIE molecules based on HSF derived exosome. The preparation method comprises the following steps: S1, preparing TTMN molecules; s2, dispersing DPPC and Chol in chloroform, performing water bath evaporation at 50 DEG C to form a thin film, hydrating the thin film in PBS, incubating to enable DPPC and Chol to form a liposome structure again, and uniformly dispersing TTMN molecules in the liposome structure to obtain TL; s3, extracting an exosome from the HSF cells; s4, loading the TL into the exosome by utilizing an electroporation technology, so as to obtain TE; s5, preparing the GelMA; and S6, mixing the GelMA with the TE, so that the TE is uniformly dispersed in the GelMA to obtain a mixed solution, injecting the mixed solution into a microneedle mold, and irradiating with 405nm laser to obtain the porous GelMA microneedle. According to the invention, high-efficiency treatment of hypertrophic scars is realized, and the system has a remarkable drug delivery effect, an innovative photodynamic therapy, a high-efficiency scar tissue remodeling effect and good biological safety and stability, and has important clinical application value and market prospect.

A method for extracting flavonoid compounds from epimedium sagittatum comprises the following steps: crushing epimedium sagittatum, carrying out reflux extraction, carrying out reduced pressure recovery to obtain an extract, suspending with distilled water, and extracting with petroleum ether, dichloromethane, ethyl acetate and n-butyl alcohol at room temperature to respectively obtain each part; the dichloromethane part is separated, eluted and combined, and components Fr.A, Fr.B, Fr.C, Fr.D, Fr.E, Fr.F, Fr.G and Fr.H are obtained; eluting the component Fr.G, and combining to obtain components Fr.G1,..., Fr.G17 and Fr.G18; eluting the component Fr.G17, and combining the eluted component Fr.G17 to obtain components Fr.G 17.1,..., Fr.G 17.15,..., Fr.G 17.19; eluting a component Fr.G 17.15, recovering under reduced pressure, and merging to obtain components Fr.G 17.15. 1,..., Fr.G 17.15. 9, Fr.G 17.15. 10,..., Fr.G 17.15. 12; the preparation method comprises the following steps: carrying out semi-preparative HPLC (High Performance Liquid Chromatography) purification on the component Fr.G 17.15. 10 to obtain a compound Epimesatine P; the preparation method comprises the following steps: carrying out semi-preparative HPLC (High Performance Liquid Chromatography) purification on a component Fr.G 17.15. 9, and collecting components with different retention times, so as to respectively obtain the compounds Epimesatine Q, Epimesatine R and Epimesatine S. The preparation method comprises the following steps of: preparing the compound Fr.G 17.15. 9; the flavonoid compound can be effectively extracted from epimedium sagittatum, can be effectively used for treating breast cancer, can be applied to preparation of anti-breast cancer drugs, and has remarkable economic and social benefits.