4809results about "Aerosol delivery" patented technology

The invention relates to a stable silk fibroin liquid condensation body, gel and a preparation method and application thereof, and belongs to the technical field of silk fibroin. The preparation method comprises the following steps: S1, dissolving silk fibroin in a lithium bromide solution, and then adding trypsin for incubation to obtain an enzyme-digested silk fibroin solution; and S2, performing low-temperature programmed centrifugal treatment including an induction stage, a nucleation stage and a liquid-liquid phase separation stage on the enzyme-digested silk fibroin solution, and cleaning to obtain the stable silk fibroin liquid-state aggregate, the method can reduce aggregate liquid drop aggregation and improve the particle size uniformity of the liquid drops.

Pharmaceutical formulations of novel indole compounds and psilocybin analogs are manufactured, provided in novel oral, transdermal, and nasal pharmaceutical compositions for use to treat neurological, mood or abuse diseases and disorders.

The invention relates to the technical field of biology, and particularly discloses a recombinant collagen III and application thereof in preparation of gel. The recombinant collagen III is designed by optimizing functional area sequences of human I-type and III-type collagen, the amino acid sequence is shown as SEQ ID No.2, and the recombinant collagen III has the characteristics of high stability, good hydrophilicity and low immunogenicity. The preparation method comprises the steps of expression vector construction, escherichia coli induced expression, affinity chromatography purification and renaturation. The recombinant collagen III can be prepared into a gel dressing and comprises sodium alginate, methylparaben and other components. Experiments show that the gel can effectively promote cell proliferation and has no cytotoxicity; in a mouse skin injury model, the collagen can relieve inflammation, accelerate wound healing and inhibit scar formation, and the effect of the collagen is superior to that of natural human III-type collagen. The invention provides a safe and efficient novel material for wound repair, and is suitable for medical dressings and tissue engineering.

Pharmaceutical formulations of novel indole compounds and psilocybin analogs are manufactured, provided in novel oral, transdermal, and nasal pharmaceutical compositions for use to treat neurological, mood or abuse diseases and disorders.

The present disclosure relates generally to antimicrobial gels. In particular, the present disclosure relates to an antimicrobial gel comprising a eutectic solvent and a gelling agent which can be used as a drug delivery vehicle for antimicrobial agents, including for the topical delivery of antimicrobial agents to the skin of a subject. The present disclosure also relates to processes for preparing the antimicrobial gel, and to methods, uses and compositions comprising the antimicrobial gel.

The invention discloses injectable hydrogel for photo-thermal adjuvant therapy of periodontitis and a preparation method, and belongs to the technical field of high polymer material preparation, the preparation method comprises the following steps: reacting MXene dispersion liquid with epigallocatechin gallate and MgSO4; then sequentially carrying out ultrasonic treatment, centrifugation, washing and freeze-drying to obtain a multifunctional nanosheet MP-Mg; adding the multifunctional nanosheet MP-Mg into an oxidized gellan gum aqueous solution, and carrying out a mixed reaction to obtain a solution A; mixing the quaternary ammonium salt chitosan aqueous solution and the carboxymethyl chitosan aqueous solution in equal volume, and reacting to obtain a solution B; and mixing the solution A and the solution B in equal volume for reaction. The hydrogel prepared by the preparation method disclosed by the invention has injectability, photo-thermal responsiveness, biological membrane penetrability, synergistic antibacterial property and long-acting anti-inflammatory property, and an innovative and practical photo-thermal adjuvant therapy platform is provided for clinical treatment of periodontitis.

The invention relates to the field of biotechnology and female health care, in particular to a stachyose composition for promoting micro-ecological regulation of lactobacillus and application of the stachyose composition, and the composition comprises stachyose and pharmaceutically acceptable auxiliary materials. The stachyose is used as prebiotics to selectively promote proliferation and colonization of the lactobacillus crispatus, the combination of 1%-5% of the stachyose and 0.1%-0.5% of the lactic acid can promote proliferation of the lactobacillus crispatus, the lactobacillus crispatus is one or more of the lactobacillus crispatus, the lactobacillus plantarum and the lactobacillus acidophilus, and the combination of 1%-5% of the stachyose and 0.1%-0.5% of the lactic acid can inhibit gardnerella and candida albicans. The stachyose composition disclosed by the invention is in the form of slow-release gel and bath foam; the composition can be used for treating or preventing bacterial vaginitis, colpitis mycotica and related symptoms, has the advantages of no antibiotics, no drug resistance, high safety and the like, and is suitable for the fields of vagina local nursing and micro-ecological regulation.

The invention discloses a hydrogel-acellular matrix composite patch containing functionalized extracellular vesicles and a preparation method of the hydrogel-acellular matrix composite patch. The composite patch comprises a hydrogel layer and an acellular pericardium matrix material layer, and an extracellular vesicle-macrophage membrane fusion body (EV-MM) is entrapped in the cross-linked hydrogel. From two aspects of improving the targeting of EVs to the cardiac infarction part and enhancing the retention of the EVs at the cardiac infarction part, the distribution condition of the EVs at the cardiac infarction part is improved, so that the action efficiency of the EVs is improved, and the treatment effect is improved.

The invention discloses a temperature-sensitive hydrogel based on Chinese prickly ash alkaloid. The temperature-sensitive hydrogel is prepared from poloxamer 407, poloxamer 188 and Chinese prickly ash alkaloid powder. The temperature-sensitive hydrogel based on the pepper alkaloid prepared by the invention has three characteristics of biological adhesion, self-healing property and slow release property, and solves the technical problems that the pepper alkaloid is poor in stability and uncontrollable in drug release.

The invention provides glycyrrhizic acid / glycyrrhetinic acid-triethanolamine composite hydrogel as well as a preparation method and application thereof, and belongs to the technical field of preparation of biological medicine carriers and composite materials. According to the invention, glycyrrhizic acid or glycyrrhetinic acid is taken as a raw material, triethanolamine is taken as a cross-linking regulating agent, and the composite hydrogel with stable mechanical properties, high drug loading efficiency and anti-inflammatory synergistic activity is prepared through a mild physical and chemical cross-linking reaction; the preparation process of the composite hydrogel does not need a toxic cross-linking agent, the reaction condition is mild, the raw materials are cheap and easy to obtain, and large-scale production can be realized; the composite hydrogel presents a multi-group ionic bond cross-linked structure, can be used as a carrier of a pH responsive drug, and has good practicability.

The invention relates to the technical field of biomedicine, in particular to stem cell exosome-loaded bi-crosslinked hydrogel as well as preparation and application thereof, and the bi-crosslinked hydrogel comprises the following components in parts by weight: 50-90 parts of a matrix polymer; 0.1 to 5 parts of an active load; 1 to 10 parts of cyclodextrin; 5 to 20 parts of modified chitosan; 0.01 to 1 part of a photoinitiator; and 0.01 to 0.5 part of growth factors. Through double-network design of physical / chemical primary cross-linking and photo-initiation secondary cross-linking, the bottleneck that the mechanical property and the machinability of the traditional single cross-linking hydrogel are difficult to consider at the same time is overcome; meanwhile, the human umbilical vein endothelial cell exosome is introduced, and efficient loading, uniform dispersion and degradation coupling slow release of the exosome are realized by virtue of beta-cyclodextrin inclusion and a double-crosslinking network. Therefore, the problems of mechanics, degradation, cross-linking speed or tissue adhesion of cross-linked hydrogel in the prior art are solved.

The invention relates to an anti-inflammatory and repair-promoting hydrogel for a uterine cavity as well as a preparation method and application of the anti-inflammatory and repair-promoting hydrogel, and relates to the technical field of biological medicines. The uterine cavity anti-inflammatory and repair-promoting hydrogel is prepared from the following raw materials: an anti-inflammatory component, a biomacromolecule with active ester and an amino high-molecular polymer. The hydrogel for resisting inflammation and promoting repair of the uterine cavity, which is prepared by the invention, has good biological safety, injectability and adhesiveness with tissues; in intrauterine adhesion prevention treatment, a three-dimensional network structure formed by crosslinking of the biomacromolecules with the active ester and the amino high-molecular polymer plays a physical isolation role, the anti-inflammatory component plays an anti-inflammatory role by inhibiting expression of an inflammation signal channel, and the biomacromolecules with the active ester and the amino high-molecular polymer achieve a synergistic treatment effect.

The invention belongs to the technical field of nano material synthesis, and particularly relates to baicalein-copper nano microgel as well as a preparation method and application thereof. According to the invention, copper ions and baicalein are coordinated to form nanowires, and the nanowires are coated with hyaluronic acid (HA) to prepare the nano microgel. The method is carried out at normal temperature, is simple in process and high in yield, and does not need toxic solvents; the obtained microgel has high stability (gastric acid resistance), targeted delivery (CD44 receptor mediation) and multiple biological activities (oxidation resistance and inflammation resistance). Animal experiments show that the pharmaceutical composition can significantly relieve ulcerative colitis, the dosage is as low as 5 mg / kg, the pharmaceutical composition is non-toxic, and a new strategy is provided for treatment of inflammatory diseases.

The invention discloses ternary synergistic nano-enzyme hydrogel as well as a preparation method and application thereof, and relates to the technical field of biomedical materials. The preparation method comprises the following steps: dissolving zinc nitrate hexahydrate and ferric nitrate nonahydrate in methanol; dissolving 2-methylimidazole in methanol, adding into a metal salt solution, stirring, and sequentially centrifuging, washing and drying to obtain Fe / ZIF-8 nano-enzyme; the preparation method comprises the following steps: adding methacrylated gelatin and hyaluronic acid into deionized water, stirring, cooling to room temperature, and refrigerating to obtain a GelMA-HA precursor solution; and adding the Fe / ZIF-8 nano-enzyme into the GelMA-HA precursor solution, stirring, injecting into a mold, and carrying out ultraviolet light cross-linking curing to obtain the ternary synergistic nano-enzyme hydrogel. According to the hydrogel disclosed by the invention, active oxygen is efficiently removed and oxygen is produced through Fe / ZIF-8 nano-enzyme, and cartilage repair is promoted in combination with lubrication and slow release functions of gelatin hydrogel, so that a new strategy is provided for treatment of osteoarthritis.

The invention provides liquiritin gel as well as a preparation method and application thereof. The liquiritin gel is prepared from the following components in concentration: 0.1 weight percent to 2 weight percent of liquiritin, 0.1 weight percent to 4 weight percent of carbomer, 0.1 weight percent to 4 weight percent of water-soluble cellulose ether compounds and 1 to 10 mL / Kg of triethanolamine. Wherein liquiritin and the water-soluble cellulose ether compound are respectively used as a main thickening agent and an auxiliary thickening agent, a synergistic thickening system is more stable and more suitable for liquiritin compared with a single thickening system or other neutralization systems, and the liquiritin and the water-soluble cellulose ether compound can synergistically adjust viscosity; triethanolamine is used as a neutralizer to neutralize carboxyl of carbomer to form gel; carbomer and a water-soluble cellulose ether compound are cooperated according to a specific ratio and can be different from a single thickening system or different neutralization systems, so that the liquiritin gel with excellent film-forming property, proper flowability and high transdermal absorption efficiency is prepared.

The invention relates to a double-framework self-developing embolism system based on iodine modified PNIPAM gel and cross-linked albumin microspheres, which simulates a brick wall-concrete effect through a microsphere-gel composite structure and synergistically improves the embolism strength. A novel TACE treatment system is designed and constructed, multiple effects of tracing, embolism, hypoxia enhanced chemotherapy and tumor microenvironment improvement are achieved through the self-developing temperature-sensitive gel-drug carrying microspheres, and the anti-tumor effect is cooperatively improved.

The invention relates to a near-infrared light response in-situ gelling and synergistic photo-thermal-photodynamic gel delivery system and a preparation method and application thereof, a mesoporous silicon coated up-conversion nano-carrier and an efficient photosensitizer are compounded to form a photo-response core unit, and then the photo-response core unit is intelligently integrated with photo-curing hydrogel to form the near-infrared light response in-situ gelling and synergistic photo-thermal-photodynamic gel delivery system. The general problem of aggregation and quenching of photosensitive molecules is relieved by utilizing the unique space confinement effect of the mesoporous structure, the ICG photo-thermal efficiency and photodynamic performance are enhanced, under precise excitation of a near-infrared light source, the system can activate a photo-crosslinking reaction through an up-conversion luminescence process, in-situ conversion from a liquid eye drop preparation to a stable gel state is achieved, and the stability of the eye drop preparation is improved. A lasting drug reservoir is formed on the surface of the cornea; synchronously excited photodynamic and photo-thermal dual-mode treatment generates a remarkable synergistic antibacterial effect, and shows an excellent removal capability on pathogens including highly drug-resistant bacteria; meanwhile, an ideal microenvironment is created for tissue repair through high biocompatibility and a stable mechanical matrix, and integrity recovery of cornea structures and functions is promoted.

The invention discloses starch-collagen composite hydrogel as well as a preparation method and application thereof. Collagen is catalyzed by microbial transglutaminase to form a covalent cross-linked network, and meanwhile, hydroxypropyl starch is introduced to construct a physical interpenetrating network through molecular chain penetration and hydrogen-bond interaction; and chondroitin sulfate is further integrated to strengthen the network structure and biological activity through electrostatic interaction. The method is mild in condition and does not need a toxic chemical cross-linking agent, the obtained composite hydrogel has an interpenetrating double-network structure and has excellent mechanical properties, enzymatic degradation resistance and cell affinity, the compression modulus of the composite hydrogel is remarkably improved, the composite hydrogel can keep structural stability for a long time in a collagenase environment, cell adhesion and proliferation can be effectively promoted, and the composite hydrogel has a good application prospect. The hydrogel can be widely applied to tissue engineering scaffolds, wound dressings, drug sustained-release carriers and cartilage repair materials.

The invention belongs to the technical field of biological materials, and particularly relates to nano composite hydrogel as well as a preparation method and application thereof. In the preparation process of the hydrogel, preparation of gelatin hydrazide and preparation of pullulan oxidation are sequentially completed, then preparation of active component loaded PLGA nanoparticles and self-assembly coating with metal-polyphenol are completed, and finally, the metal-polyphenol coated PLGA nanoparticles are mixed with gelatin hydrazide and pullulan oxidation to form gel in situ. The hydrogel has the characteristics of injectability, self-healing, wet adhesion and the like, the metal-polyphenol layer has the effects of resisting inflammation, resisting oxidation and promoting angiogenesis and generates photo-thermal response to near infrared rays, on-demand controllable release of the drug-loaded nanoparticles is achieved, the composite hydrogel can improve the wound surface microenvironment, promote angiogenesis and inhibit inflammation and scar formation, and the drug-loaded nanoparticles can be applied to wound healing. The ointment is suitable for burns, scalds and various complex wounds.

The invention discloses injectable double-drug delivery hydrogel and a preparation method thereof, and belongs to the technical field of biomedical materials. According to the method, two nano microspheres with different charges are respectively prepared by utilizing the characteristics of A-type gelatin and B-type gelatin, a negatively charged medicine EGCG is specifically carried by the positively charged A-type gelatin, and a positively charged medicine SAG is carried by the negatively charged B-type gelatin microsphere; and finally, mixing the two gelatin drug-loaded microspheres into a material taking gelatin and zirconium ions as a substrate to form the composite hydrogel. The hydrogel realizes immune regulation and osteogenesis regulation, also realizes long-acting release of the two drugs, effectively solves the problems of bone resorption, difficulty in healing and the like of host tissues through synergistic regulation of inflammation and mobilization of osteogenesis genes, realizes personalized treatment, and improves the clinical treatment effect and efficiency.

The invention relates to the technical field of functional food and biological medicine, in particular to a modified pseudo-ginseng exosome and an intestinal conditioning gel composition and application thereof. According to the gel beverage, an exosome derived from panax notoginseng callus cultured in a laboratory is used as a core active component, is modified through phosphatidylserine-aminated pectin-genipin and is embedded into a hydrogel matrix composed of carboxymethyl chitosan and sodium alginate, so that the exosome is protected from being degraded in the stomach, and the activity of the exosome in the stomach is improved. The exosome can be effectively released under the intestinal pH and flora environment, so that the targeted delivery of the intestinal tract is realized, and the preparation method is suitable for improving the intestinal inflammation and maintaining the intestinal health. The invention can effectively solve the technical problems of uncontrollable raw material quality and safety, low oral delivery stability and active ingredient availability and poor medication compliance of the existing plant exosome.

The invention relates to injectable temperature-sensitive hydrogel loaded with traditional Chinese medicine active exosomes, and belongs to the technical field of clinical treatment of diabetic foot refractory wounds, and the injectable temperature-sensitive hydrogel is obtained by poloxamer 407 hydrogel and a hyaluronic acid loaded composite carrier; the composite carrier body is formed by coating a traditional Chinese medicine active exosome with a coating solution; the traditional Chinese medicine active exosome is obtained by inducing adipose-derived mesenchymal stem cells with a modified astragalus extract. The injectable temperature-sensitive hydrogel is obtained by loading a composite carrier with poloxamer 407 hydrogel and hyaluronic acid, the poloxamer 407 and the hyaluronic acid quickly form a physical barrier on a wound surface after being injected, a moist healing environment is provided, and continuous and slow release of exosome and active ingredients is realized by means of the temperature-sensitive characteristic and the network structure of the poloxamer 407 and the hyaluronic acid, so that the wound healing effect is improved. And over-high local concentration or premature loss caused by sudden release of the medicine is avoided.

Systems, compositions, and methods related to injectable hydrogels are generally described. In some embodiments, a composition comprises a first polymer and a plurality of particles comprising a second polymer capable of forming a hydrogel at elevated temperatures, such as body temperature. The plurality of particles (e.g., hydrogel particles) may comprise an active substance, such as a biological material and / or a therapeutic agent. Together, the first polymer and the second polymer may interact with each other (e.g., the first polymer may interpenetrate the second polymer, and / or the first polymer may cross-link with the second polymer) at elevated temperatures to form a hydrogel. Interactions between the first and second polymer, and / or between hydrophobic domains thereof, may facilitate the relatively slow and / or controlled release of the active substance. In some embodiments, the plurality of particles may have advantageously high loadings of biological materials (e.g., antibodies, proteins, peptides).

The invention discloses a collateral-dredging and pain-relieving gel plaster and a preparation method thereof. The collateral dredging and pain relieving ointment is prepared from the following raw materials in percentage by mass: camphor, borneol, menthol, absolute ethyl alcohol, collateral dredging and pain relieving volatile oil, a surfactant, collateral dredging and pain relieving extract, tartaric acid, purified water, partially neutralized sodium polyacrylate, a cross-linking agent, an adhesive, glycerol, EDTA-2Na (Ethylene Diamine Tetraacetic Acid) and a filling agent. The plaster disclosed by the invention is high in application comfort, breathable, free from aching feeling after being uncovered, free from residue on skin and not sticky when being in contact with sweat; the use of an organic solvent is basically avoided in the production process; the matrix contains components such as water and glycerol, has the effects of moistening the skin and promoting skin hydration, and is free of irritation and sensitization to the skin; repeated uncovering and pasting can be achieved, and the viscosity influence is small.

The invention discloses a method for promoting skin repair by assisting exosome transdermal delivery through a sponge spicule. The method comprises the following steps: pretreating skin by using a composition containing the sponge spicule; mixing the exosome vesicle freeze-dried powder with a redissolving medium at 5-40 DEG C, and then applying the mixture to the skin; the exosome vesicle freeze-dried powder is obtained by performing membrane fusion on exosome and liposome to obtain exosome vesicles and then performing freeze drying. According to the invention, the skin is pretreated by using the composition containing the sponge bone needle, the skin barrier resistance is reduced, the skin repair response is stimulated, and then the exosome vesicles are re-dissolved and then applied to the skin surface, so that the transdermal utilization rate of exosome contents is promoted. The exosome vesicle provided by the invention has good in-vitro storage stability, and after the exosome vesicle is stored at 0-5 DEG C for 180 days, the activity retention rate after redissolution is 80% or above.

The present invention aims to provide a composition having excellent anti-aging properties. [Solution] A composition characterized by containing nicotinamide mononucleotide and at least one selected from the group consisting of pine bark, banaba, and fulvic acid.

The invention discloses a temperature-sensitive hydrogel loaded with mesenchymal stem cell exosome as well as a preparation method and application of the temperature-sensitive hydrogel, and belongs to the technical field of biological medicines. The method comprises the following steps: (1) adding poloxamer 407 into a pre-cooled dispersion medium to enable the final mass concentration to be 20-30%, and oscillating and dissolving at 4 DEG C overnight to obtain poloxamer hydrogel; (2) collecting a mesenchymal stem cell culture supernatant, and then centrifuging, wherein the centrifuging condition is 200 * g * 15 minutes to 2000 * g * 20 minutes to 12000 * g * 20 minutes; filtering the supernatant, concentrating by using an ultrafiltration tube, further performing ultracentrifugation on the concentrated solution, re-suspending the precipitate by using DPBS, centrifuging again, and re-suspending the finally obtained exosome precipitate by using DPBS to obtain an exosome suspension; the ultracentrifugation condition is as follows; and (3) taking the poloxamer hydrogel prepared in the step (1), adding the exosome suspension obtained in the step (2) under an ice-water bath condition, and uniformly mixing to obtain the poloxamer temperature-sensitive hydrogel.

The invention is applicable to the technical field of polymer biomedical hydrogel materials, and provides a preparation method and application of multifunctional agarose-based particle composite hydrogel based on liquid-liquid phase separation, and the agarose-based particle composite hydrogel is a product formed by introducing microsphere particles into an agarose hydrogel body matrix; the microsphere particles are formed by a prepolymer and sodium lignin sulfonate through phase separation mediated by a composite behavior based on non-covalent interaction; the prepolymer is obtained by carrying out amine-epoxy reaction on an amino compound and an epoxy compound in water. According to the prepared agarose-based particle composite hydrogel, a microsphere particle structure is formed on the surface of an agarose matrix while positively charged amino groups and sodium lignin sulfonate are introduced, so that the multifunctional agarose-based particle composite hydrogel is prepared.

The invention discloses a preparation method and application of tetrastigma hemsleyanum exosome vesicles with an antibacterial function, and relates to the technical field of biological medicines. The invention relates to a method for extracting plant exosome vesicles from Tetrastigma hemsleyanum, in particular to a method for extracting the plant exosome vesicles from Tetrastigma hemsleyanum, the method is simple and convenient to operate and low in cost, and the plant exosome vesicles can be efficiently obtained, so that the application of the plant exosome vesicles in industrial production has potential, and the method is suitable for industrial production. The resource consumption and the cost overhead in the extraction process are reduced; the exosome vesicles protect the active components: the extracted exosome vesicles can effectively protect the active components (such as small RNA, lipid and the like) in the radix tetrastigme from being degraded, and the exosome vesicles have a natural protection effect, can enhance the stability of the components and ensure that the components are not damaged in the use process, so that stronger biological activity is exerted.

The invention belongs to the technical field of external preparations, and provides compound itching-relieving cream as well as a preparation method and application thereof. The compound itching-relieving ointment is prepared from the following components in parts by weight: 3 to 6 parts of rhizoma coptidis, 9 to 15 parts of radix scutellariae, 9 to 15 parts of radix et rhizoma rhei, 3 to 6 parts of herba schizonepetae, 3 to 6 parts of herba menthae, 1 to 3 parts of cortex moutan, 1 to 3 parts of sandalwood and 0.5 to 1 part of borneol. The compound antipruritic ointment has the effects of cooling, relieving itching, detoxifying and diminishing swelling, and is used for treating itching and eczema dermatitis itching caused by biting of various small insects such as mosquitoes and the like. According to the compound antipruritic ointment disclosed by the invention, a plurality of components are in synergistic interaction, so that a multi-target and multi-layer treatment effect is realized. The traditional Chinese medicine composition can be used for cooling, relieving itching, detoxifying and diminishing swelling from the source while quickly relieving itching, and a comprehensive and effective solution is provided for itching.