579 results about "Reprogramming" patented technology

The present invention provides methods for de-differentiating somatic cells into stem-like cells without generating embryos or fetuses. More specifically, the present invention provides methods for effecting the de-differentiation of somatic cells to cells having stem cell characteristics, in particular pluripotency, by introducing RNA encoding factors inducing the de-differentiation of somatic cells into the somatic cells and culturing the somatic cells allowing the cells to de-differentiate.

A protocol for optimizing the energy for code upload to sensors in a wireless sensor network and speeding up the dissemination if multiple sources of code are available. Energy optimization is achieved by equipping each node with limited non-local topology information, which it uses to determine the time when it can go to sleep since code is not being distributed in its vicinity. Another aspect of the invention is a protocol that facilitates secure upload of code in the wireless sensor network. The secure communication protocol divides the sensor field into control groups each with a control node, and manages data exchange between nodes within a control group through the mediation of a control head which provides the common key. The keys are refreshed periodically and the control nodes are changed periodically to enhance security. The protocol facilitates secure communication between sensor nodes despite the compromise of any number of other nodes in the network.

This invention generally relates to a method for developing, generating and selecting human embryonic stem (hES)-like pluripotent cells using transgenic expression of intronic microRNA-like RNA agents. More particularly, the present invention relates to a method and composition for generating a non-naturally occurring intron and its intronic components capable of being processed into mir-302-like RNA molecules in mammalian cells and thus inducing certain specific gene silencing effects on differentiation-related and fate-determinant genes of the cells, resulting in reprogramming the cells into a pluripotent embryonic stem (ES)-cell-like state. The ES-like cells so obtained are strongly express hES cell markers, such as Oct3 / 4, SSEA-3 and SSEA-4, and can be guided into various tissue cell types by treating certain hormones and / or growth factors under a feeder-free cell culture condition in vitro, which may be used for transplantation and gene therapies. Therefore, the present invention offers a simple, effective and safe gene manipulation approach for not only reprogramming somatic cells into ES-like pluripotent cells but also facilitating the maintenance of pluripotent and renewal properties of ES cells under a feeder-free cell culture condition, preventing the tedious retroviral insertion of four large transcription factor genes into one single cell as used in the previous iPS methods.

Methods and composition of induction of pluripotent stem cells are disclosed. For example, in certain aspects methods for generating essentially vector-free induced pluripotent stem cells with cell signaling regulators are described. Furthermore, certain aspects of the invention provide novel compositions comprising induced pluripotent stem cells essentially free of exogenous retroviral vector elements in the presence of a medium comprising signaling inhibitors. In certain aspects, feeder-free episomal reprogramming methods may be provided.

The invention relates to a remotely programmable personal medical device, in particular a programmable implantable medical device, e.g., a cardiac pacemaker, a defibrillator, a cardioverter or the like. In addition, the invention relates to an arrangement for remote programming of such a personal medical device and a method for remote programming of a programmable personal medical device.

The present disclosure relates to methods of and systems for modifying the transcriptional regulation of stem or progenitor cells to promote their differentiation or reprogramming of somatic cells. Further, the labeling and editing of human genomic loci in live cells with three orthogonal CRISPR / Cas9 components allow multicolor detection of genomic loci with high spatial resolution, which provides an avenue for barcoding elements of the human genome in the living state.

A system and method for managing remote reprogramming of flash memory in a vehicle electronic control unit. A vehicle state manager process is used to first determine if the vehicle conditions are suitable for reprogramming of a particular ECU and, if so, the vehicle state manager then maintains the proper vehicle configuration during the reprogramming operation. The system and method can be used to automatically reprogram a vehicle ECU using new programming received by digital satellite broadcast or other wireless transmission to the vehicle.

This invention describes unique treatment methods and innovative articles that can be placed in a human or animal body to enable controlled destruction of diseased tissue. The methods include destruction of diseased cells and tissues by magnetically controlled motion and an externally controllable drug delivery process with a capability to start and stop the drug delivery at any time, for any duration. This invention provides two approaches to diseased cell destruction, (1) magneto-mechanical disturbance of cell structure (e.g. cancer cells) for cell lysis and (2) magnetically activated drug release at local regions (e.g. tumors) from a magnetic-particle-containing drug reservoir. The invention also provides combinations of both the above treatments for dual therapy. It further combines one or both of the treatments with magnetic hyperthermia for multifunctional cell destruction therapy. The approaches can be combined with magnetic MRI for monitoring the accuracy of placement as well as for following up the cancer destruction progress and appropriate reprogramming of the magneto-mechanical therapy and remote-controlled drug release.

The present invention generally relates to a method for developing, generating and selecting tumor-free embryonic stem (ES)-like pluripotent cells using electroporation delivery of an inducible tumor suppressor mir-302 agent into mammalian cells. More particularly, the present invention relates to a method and composition for generating a Tet-On / Off recombinant transgene capable of expressing a manually re-designed mir-302 microRNA (miRNA) / shRNA agent under the control of doxycyclin (Dox) in human somatic / cancer cells and thus inducing certain specific gene silencing effects on the differentiation-associated genes and oncogenes of the cells, resulting in reprogramming the cells into an ES-like pluripotent state.

The present invention provides compositions and methods for reprogramming somatic cells using purified RNA preparations comprising single-strand mRNA encoding an iPS cell induction factor. The purified RNA preparations are preferably substantially free of RNA contaminant molecules that: i) would activate an immune response in the somatic cells, ii) would decrease expression of the single-stranded mRNA in the somatic cells, and / or iii) active RNA sensors in the somatic cells. In certain embodiments, the purified RNA preparations are substantially free of partial mRNAs, double-stranded RNAs, un-capped RNA molecules, and / or single-stranded run-on mRNAs.

A system for managing a network of environmental control units (ECUs) enables an ECU user to more easily move from one living environment to another. The system allows an ECU to be conveniently configured based on the contents of an ECU configuration profile database that stores ECU user profiles and living environment profiles, without manual reprogramming of specific user or living environment characteristics. An ECU configuration profile database is maintained that stores user profiles made up of user specific ECU configuration information, and living environment profiles made up of living environment specific ECU configuration information. Each user profile contains user specific characteristics. Each living environment profile contains living environment characteristics. Individual user profiles and living environment profiles are independently stored in and retrieved from the ECU configuration profile database. The ECU configuration profiles stored in the ECU configuration profile database can each be uploaded from, and / or downloaded onto, any ECU in the network of interconnected ECUs through an administrator tool application program that provides a graphical user interface to an administrator user.

Systems and methods are disclosed for teaching a person how to perform a task within a computer program with at least one graphical user interface object. A script is formulated that contains step-by-step instructions indicating to a person how to perform a task within a computer program through interacting with at least one graphical user interface object of the computer program. The script is formulated by reference to an entity map, which contains programming instructions for accessing at least one graphical user interface object of the program to perform a step of the task. The script is executed by displaying a partial graphical overlay on top of an actual, non-simulated graphical user interface of the program. Each graphical user overlay instructs a user how to provide pertinent user input to graphical user interface objects of the computer program. The partial graphical overlay is positioned adjacent to the pertinent graphical user interface object of the program regardless of the position of that object without any reprogramming of the underlying computer program.

The present disclosure relates to methods for dedifferentiating and transdifferentiating recipient cells, preferably human somatic cells. These methods minimize the risk of undesired genome sequence alteration. These methods employ reprogramming factors, which may be used alone or in certain combinations with one another. These methods have application especially in the context of cell-based therapies, establishment of cell lines, and the production of genetically modified cells.

Systems and methods are disclosed for teaching a person how to perform a task within a computer program with at least one graphical user interface object. A script is formulated that contains step-by-step instructions indicating to a person how to perform a task within a computer program through interacting with at least one graphical user interface object of the computer program. The script is formulated by reference to an entity map, which contains programming instructions for accessing at least one graphical user interface object of the program to perform a step of the task. The script is executed by displaying a partial graphical overlay on top of an actual, non-simulated graphical user interface of the program. Each graphical user overlay instructs a user how to provide pertinent user input to graphical user interface objects of the computer program. The partial graphical overlay is positioned adjacent to the pertinent graphical user interface object of the program regardless of the position of that object without any reprogramming of the underlying computer program.

A method for treating cells and / or nuclear transfer units and / or stem cells in culture with such compounds, individually or in combinations, is described. The method results in a globally hypomethylated genome and a restoration of cell differentiation and / or developmental potential, or potentiality. In addition, a method for the in vitro production of reprogrammed cells which have had differentiation potential (totipotential, pluripotential, or multipotential) restored by demethylating the genome is described.

Gaming machines may be remotely reconfigured and / or reprogrammed, data remotely collected, and a comparison performed between at least two different configurations. Such may be done to determine optimal configurations of gaming machines for various periods (e.g., days, times, months, Holidays). The reconfiguration and / or reprogramming may, for example, change a minimum or maximum wager limit, change a payout schedule, change an aspect of a bonus, progressive or other jackpot, and / or change allocation an of gaming machines between Class II and Class III. Reports may be produced indicative of the effects of the reconfiguration or reprogramming.

The present invention describes the use of pre-trans-splicing molecules (PTMs) to reprogram human normal and diseased somatic cells into pluripotent stem cells using spliceosome-mediated RNA trans-splicing. More specifically, the present invention describes the use of the SMaRT™ technology to repair or reprogram the newly induced diseased pluripotent stem cells.

Human somatic cells are reprogrammed to become induced pluripotent stem cells (iPS cells) by the introduction of a minicircle DNA vector. Cells of interest include adipose stem cells.

The present disclosure relates generally to novel methods and compositions for using engineered reprogramming factor(s) for the creation of induced pluripotent stem cells (iPSCs) through a kinetically controlled process. Specifically, this disclosure relates to establishing combinations of reprogramming factors, including fusions between conventional reprogramming factors with transactivation domains, optimized for reprogramming various types of cells. More specifically, the exemplary methods disclosed herein can be used for creating induced pluripotent stem cells from various mammalian cell types, including human fibroblasts. Exemplary methods of feeder-free derivation of human induced pluripotent stem cells using synthetic messenger RNA are also disclosed.

A programmable weighing scale and a system and method for programming values of adjustable operating parameters used in generating the displayed output from the weighing scale. The weighing scale is placed into a restricted-access programming mode, which allows an authorized programmer to select values of operating parameters that allow the programmer to initially set or change the service characteristics of the scale. Operating parameters that can be varied include variables used in sampling the weighing scale's transducer electrical output, mathematically processing that output, and displaying the results. Once the operating parameter values have been selected, the weighing scale will utilize those values until the weighing scale undergoes a subsequent reprogramming.

Therapeutically programmed cells and methods for making such cells are provided. Therapeutically programmed cells are stem cells which have been matured such that they represent either a more differentiated state or a less differentiated state after contact with stimulatory factors. The therapeutically reprogrammed cells are suitable for cellular regenerative therapy and have the potential to differentiate into more committed cell lineages. Additionally, hybrid stem cells suitable for therapeutic reprogramming and cellular regenerative therapy are provided.

A set of non-volatile storage elements undergoes initial programming, after which a reprogramming, with higher verify levels, is performed in non-real time, such as when a control enters a standby mode, when no other read or write tasks are pending. The reprogramming can program pages in the set one at a time, stopping at a page boundary when another read or write task is pending, and restarting when the control become available again. Status flags can be provided to identify whether a page and / or the set has completed the reprogramming. In another aspect, a higher pass voltage is applied to unselected word lines during the reprogramming. In another aspect, an error count is determined using a default set of read voltages, and an alternative set of read voltages is selected if the count exceeds a threshold.

The invention provides methods for cloning mammals that allow the donor chromosomes or donor cells to be reprogrammed prior to insertion into an enucleated oocyte. The invention also features methods of inserting chromosomes or nuclei into recipient cells.

The present invention provides a variety of nucleic acid based therapeutics and methods of use thereof which are effective to beneficially reprogram diseased cells such that they exhibit more desirable phenotypes. Also provided are compositions and methods to reprogram normal cells for medical and commercial purposes.

A method is for programming a flash memory device which includes a plurality of memory cells storing multi-bit data representing one of a plurality of states. The method includes programming the multi-bit data into selected memory cells of the plurality of memory cells, the programming including a first verify-reading operation performed by a first verifying voltage, determining whether to execute a reprogramming operation for each of the selected memory cells, and reprogramming the selected memory cells in accordance with the determination. The reprogramming of the selected memory cells includes a second verify-reading operation performed by a second verifying voltage, the second verifying voltage being higher than the first verifying voltage.

Disclosed herein are methods to select for the generation of mouse and human pluripotent stem cells during developmental reprogramming. The methods described herein relate to the selection of induced pluripotent stem cells, i.e., pluripotent stem cells generated or induced from differentiated cells without a requirement for genetic selection. Described herein are particular embodiments for selection of reprogrammed cells based on 1) colony morphology, or 2) X chromosome reactivation in female cells.

A system for managing a network of environmental control units (ECUs) enables an ECU user to more easily move from one living environment to another. The system allows an ECU to be conveniently configured based on the contents of an ECU configuration profile database that stores ECU user profiles and living environment profiles, without manual reprogramming of specific user or living environment characteristics. An ECU configuration profile database is maintained that stores user profiles made up of user specific ECU configuration information, and living environment profiles made up of living environment specific ECU configuration information. Each user profile contains user specific characteristics that reflect an associated user's disability, physical characteristics and / or preferences. Each living environment profile contains living environment characteristics that describe the living environment devices located in an associated living environment, including information regarding how those devices can be controlled by an ECU. Individual user profiles and living environment profiles are independently stored in and retrieved from the ECU configuration profile database. The ECU configuration profiles stored in the ECU configuration profile database can each be uploaded from, and / or downloaded onto, any ECU in the network of interconnected ECUs through an administrator tool application program that provides a sophisticated and powerful graphical user interface to an administrator user.

Disclosed herein are cell culture compositions, for example, pancreatic cell culture compositions, derived from dedifferentiated human reprogrammed pluripotent stem cells, such as induced pluripotent stem (iPS) cells, and methods for producing and using such cell culture compositions.

A high-speed lookup table is designed using Rapid Single Flux Quantum (RSFQ) logic elements and fabricated using superconducting integrated circuits. The lookup table is composed of an address decoder and a programmable read-only memory array (PROM). The memory array has rapid parallel pipelined readout and slower serial reprogramming of memory contents. The memory cells are constructed using standard non-destructive reset-set flip-flops (RSN cells) and data flip-flops (DFF cells). An n-bit address decoder is implemented in the same technology and closely integrated with the memory array to achieve high-speed operation as a lookup table. The circuit architecture is scalable to large two-dimensional data arrays.