222 results about "Cryoprotectant" patented technology

The invention belongs to the technical field of bone tissue low-temperature storage, and particularly relates to a deep low-temperature storage method for retaining bone tissue activity, which comprises the following steps: A1, acquiring a bone tissue sample, transporting the bone tissue sample to a treatment center in a cold chain manner, pre-treating the bone tissue, cleaning periosteum, putting the bone tissue sample into negative-pressure ultrasonic purified water for cleaning, inactivating with alcohol, flushing with sterile PBS (Phosphate Buffer Solution), and cleaning the bone tissue; a2, preparing a cryoprotectant, immersing the bone tissue into the cryoprotectant, and maintaining the temperature through an ice-water bath; a3, after the cryoprotectant enters the bone tissue, the bone tissue is placed in pre-freezing equipment, and the bone tissue is pre-frozen; a4, the pre-frozen bone tissue is taken out and then put into a liquid nitrogen storage container, and low-temperature storage of the bone tissue is started; through the treatment of the series of methods, the formation of ice crystals can be reduced, and the icing of water in cells and tissues is promoted to become more uniform and smaller, so that the damage to bone tissue cells is reduced.

The invention provides application of a protein deacetylase inhibitor NAM in ultralow-temperature cryopreservation of poultry semen, and belongs to the technical field of poultry breed conservation. The protein deacetylase inhibitor NAM is used as a cryoprotectant or is combined with other cryoprotectants for use, and the protein deacetylase inhibitor NAM is used as an effective component, so that the ultralow-temperature cryopreservation efficiency of poultry can be improved. Wherein the protein deacetylase inhibitor NAM can inhibit sperm cell deacetylation modification so as to maintain a high acetylation modification level, improve the ability of sperms to respond to ultralow temperature freezing stimulation and improve the fertilization ability of frozen-remelted sperms.

The present invention provides methods for cryopreserving a population of cells with improved cell viability. In some aspects, the method comprises contacting a population of cells with a peptoid polymer comprising one or more polar peptoid monomers, e.g., formulated in a cryoprotectant solution, and cooling the population of cells at a temperature of from 0° C. to about −20° C. for a time period of at least about 3 hours to produce a population of supercooled cells. The supercooling methods of the present invention provide excellent post-thaw cell survival and recovery. In certain embodiments, the population of cells is present in a tissue or an organ that is cryopreserved by performing the supercooling methods of the present invention.

The present disclosure includes hyperbranched polyether polyols, such as hyperbranched poly(3-(oxiran-2-ylmethoxy) propane-1,2-diol) (HPOD), methods for their preparation and uses thereof, for example, as a cryoprotectant, lyoprotectant or stabilizer.

A coupled enzyme reaction point of care assay system detects and measures an active enzyme biomarker from a patient's blood sample. The assay system has a point of care blood collector for collecting blood into a blood collection vial. A biomarker detection mechanism has at least three fluid flow entities. Each fluid flow entity has a fluid input zone and a corresponding reaction zone. The fluid flow entities include a test strip, a negative control strip and a positive control strip. The reaction zones include components from a multiplicity of substrates, co-factors, buffers and cryoprotectants, and a multiplicity of tethered enzymes. At least one of the tethered enzymes is an enzyme that produces luminescence. The tethered enzymes are adapted to react to a constituent which may be a biomarker. A photonic luminescence reader measures light emitted from an active enzyme biomarker present in the patient's blood.

The invention discloses a cryoprotectant which comprises glycerol, skimmed milk powder, mannitol, trehalose, vitamin C, fructo-oligosaccharide, inulin and sterile water. By optimizing the components of the cryoprotectant, the activity of the coprophilous fungi can be effectively protected, the survival rate of the intestinal coprophilous fungi in the freezing process is remarkably increased, all the used components are food-grade edible components and have a wide application range and economic effects, the invention further discloses a coprophilous fungi freezing preservation method adopting the cryoprotectant, and the cryoprotectant has good application prospects. The method is simple, easy to operate and suitable for large-scale production and application.

The invention belongs to the field of marine food processing, and provides a marine food containing yellow croaker and a preparation method thereof.The marine food containing yellow croaker is prepared by adopting collaborative design of a minced yellow croaker protein-polysaccharide composite nano colloid powder intermediate and low-molecular fish collagen, through microbial transglutaminase cross-linking and cryoprotectant compounding regulation and control, and the marine food containing yellow croaker and the preparation method of the marine food containing yellow croaker are obtained. A minced fillet protein continuous phase network structure with uniformly dispersed nano-particles is constructed, the gel strength is 900-1300 g.cm, the gel strength retention rate is not lower than 80% after 6 times of freezing and thawing cycles are frozen for 3-5 times, the unfreezing drip loss is not higher than 8 wt%, the hardness is 1500-2500 g, and the taste characteristics of high strength and good elasticity are obtained. The technical problems that high gel strength and freeze-thaw stability of a traditional surimi product are difficult to consider, the taste is worse easily due to improvement of mechanical properties, nano-particles are difficult to uniformly disperse in a high-solid-content system and the like are solved, and the surimi product has wide application value in the fields of surimi products, quick-frozen prepared foods and marine functional foods.

The invention discloses a biochemical multi-item liquid quality control product containing 67 analytes and a preparation method of the biochemical multi-item liquid quality control product, and belongs to the technical field of biochemical quality control products, non-analytes of the quality control product comprise a cryoprotectant compound with the addition amount of 0.1-1%, the cryoprotectant compound comprises antifreeze protein and a protein stabilizer, and the antifreeze protein and the protein stabilizer are added into the quality control product. The mass ratio of the antifreeze protein to the protein stabilizer is (0.1: 1)-(0.5: 1); the biochemical multi-liquid quality control product is frozen liquid. According to the invention, antifreeze protein and elongation factor 1-alpha homologous protein cooperate through physical-chemical dual pathways, complete-cycle protection is provided for protein and enzyme in a quality control product aiming at'mechanical factor 'and'oxidation factor' of freezing damage, and inter-batch consistency and storage stability can be improved; and errors caused by redissolution can be avoided, the detection convenience and accuracy of inspectors are greatly improved, and the labor and time cost of a laboratory is reduced.

The invention discloses a stable oncolytic adenovirus composition, which contains adenovirus or recombinant oncolytic adenovirus modified based on the adenovirus, and also contains a nonionic surfactant (virus stabilizer), a cryoprotectant, an osmotic pressure regulator and a buffer substance. The composition can still keep stable activity in low-temperature storage (lower than-70 DEG C), cold-chain transportation, repeated freezing and thawing and temporary storage at room temperature (10-30 DEG C). The invention also relates to application of the composition in medicine.

The present disclosure is concerned with peptides comprising alanine residues and glycosylated residues (e.g., glycosylated hydroxypropline residues, a combination of glycosylated hydroxyproline residues and glycosylated threonine residues) in a particular ratio (e g., from about 3:2 to about 4:1), wherein the peptide has a minumum chain length, such as, for example a chain length of at least 30 ammo acid residues. The disclosed peptides beneficially inhibit ice crystal formation, and, therefore, offer utility in a wide range of applications, including, but not limited to biomedical cry opreservation, food technology, agriculture, cosmetics, and building materials. Thus, the disclosed peptides can be formulated into a composition (e.g., a cryoprotectant composition, an agricultural formulation, a cosmetic composition) or a food product, or, alternatively, can be attached or coated onto a surface for use in structural applications. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention.

The invention relates to the technical field of organ transplantation, and discloses organ preserving fluid, application and organ dynamic supercooling perfusion equipment. The organ preserving fluid is prepared from ribose, glucose, mannitol, lidocaine, adenosine, a high-molecular polymer, L-proline, trehalose, inorganic salt and organic salt. The high-molecular polymer comprises one of hydroxyethyl starch, polyethylene glycol and albumin. According to the organ preserving fluid disclosed by the invention, the freezing point of the perfusate is reduced through the micromolecular cryoprotectant, and meanwhile, the micromolecular cryoprotectant and the high-molecular polymer jointly inhibit formation and growth of ice crystals; the problem of liquid preservation at the temperature below zero is solved from the two dimensions of physics (reducing the freezing point and inhibiting ice crystals) and biology (maintaining cell viability), and feasibility is provided for long-time low-temperature transfer and transplantation of organs. The preserving fluid can be used for static preservation or perfusion preservation of various organs such as kidney, liver, heart, pancreas, lung, small intestine, skin and the like.

The present invention relates to compositions for storing microbiota, in particular microbiota compositions comprising inulin and maltodextrin as cryoprotectants / lyoprotectants. The present invention also relates to dosage forms and methods of treating disorders and diseases by administering the composition to a patient in need thereof.

The present disclosure provides a liquid cryoprotectant having a lactam moiety structure of wherein the lactam having at least one hydroxyl functional group; wherein Y is an alkylene or alkenylene group comprising 2 to 50 carbon atoms; wherein 2 to 4 carbon atoms reside in the lactam ring between the I group and the II group; Q is selected from the group consisting of functionalized and unfunctionalized alkylenes of linear, branched, or cyclic structure, rylenes, and combinations thereof, wherein the aforementioned groups may or may not contain oxygen atom(s); and R is selected from the group consisting of hydrogen and functionalized and unfunctionalized alkyl, cycloalkyl, alkenyl, and aryl groups, wherein the alkyl, cycloalkyl, alkenyl, and aryl groups may or may not contain heteroatoms. The present disclosure also provides a composition for cryopreservation having a lactam moiety structure.

The invention discloses a novel antifreeze polypeptide with a clear structure and excellent performance and a protection method of cryopreserved cells. The core structure of the polypeptide is formed by covalently connecting two functional modules, the N end is a unit with ice crystal binding performance, the C end is a super-hydrophilic unit, and the two functional modules are bridged through a flexible linker. Experimental verification shows that the synthetic polypeptide shows excellent anti-freezing activity, can significantly inhibit nucleation, growth and recrystallization processes of ice crystals, and has particularly outstanding ice recrystallization inhibition activity. The novel antifreeze polypeptide shows excellent biocompatibility, and is suitable for low-temperature cryopreservation of cells sensitive to freezing damage and other living microbial preparations. Results show that the polypeptide can greatly improve the freeze-drying survival rate of cells and the activity of the cells after recovery, is non-toxic to the cells, and shows the potential of replacing a traditional cryoprotectant. Therefore, the method has wide application prospects in food industry, biological medicine, agricultural breeding and low-temperature preservation technologies of biological sample libraries.

The invention relates to the technical field of cell biology, in particular to an iNKT cell cryopreservation solution and a method for cryopreserving iNKT cells by using the cryopreservation solution. The invention provides an immune cell cryopreservation solution. The immune cell cryopreservation solution comprises human serum albumin, a basic low-temperature protection solution and an optional human platelet lysate. The immune cell freezing medium does not contain animal-derived components except for human bodies, and has the advantages of simple components and good stability. According to the cryopreservation liquid disclosed by the invention, through the combined action of the basic low-temperature protection liquid (such as CryoStor10 (CS10)) and the human serum albumin, the motility rate after cell recovery is improved, and the damage to immune cells (such as iNKT) in the cryopreservation process is reduced. After a small amount of human platelet lysate is further added, the survival rate of cell resuscitation and the amplification capacity can be further improved.

A microfluidic device that includes a trap configured to retain an oocyte or fertilized species, a plurality of microchannels in fluid communication with the trap, and a plurality of valves, where each valve is configured to control fluid flow through a corresponding set of one or more of the plurality of microchannels. The microfluidic device further includes a plurality of fluidic timer circuits each configured to produce a corresponding first time-dependent fluid pressure signal, and a fluidic logic gate circuit configured to receive the first time-dependent pressure signals and to produce a plurality of second time-dependent fluid pressure signals, where each second time-dependent fluid pressure signal controls operation of a corresponding one of the valves.

The present disclosure is concerned with peptides comprising alanine residues and glutamic acid residues in a particular ratio (e.g., from about 1 : 1 to about 4; I ). wherein the peptide has a minimum chain length, such as, for example a chain length of at least 15 or at least 20 amino acid residues. The disclosed peptides beneficially inhibit ice crystal formation, and, therefore, offer utility in a wide range of applications, including, but not limited to biomedical cryopreservation. food technology, agriculture, cosmetics, and building materials. Thus, the disclosed peptides can be formulated into a composition (e.g.. a cryoprotectant composition, an agricultural formulation, a cosmetic composition) or a food product, or, alternatively, can be attached or coated onto a surface for use in structural applications. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention.

Large volume cellular material may be preserved by combining the cellular material with a cryoprotectant formulation / medium / solution containing at least one mNP and then subjecting the cellular material to a vitrification preservation protocol including nanowarming. This preservation method is particularly effective for cartilage tissues.

Provided in the present invention is a cell freezing medium for clinical use. In particular, the cell freezing medium of the present invention comprises the following components: (1) human albumin; (2) cryoprotectant: the cryoprotectant comprises a combination of one or more of dimethyl sulfoxide, glycerol, and ethylene glycol; (3) a saline buffer; wherein the salt buffer is a solution containing Na+, K+, Mg+, Cl−, and CH3COO− ions; (4) a vitamin; and (5) an amino acid, wherein the human albumin concentration is 1%-20% (w / v). The cell, after long-term cryopreservation with the freezing medium of the present invention, has a high viability, and the cellular efficiency maintains a high uniformity. The grade of purity of the freezing medium of the present invention is the pharmaceutical grade or USP grade; and the freezing medium is safe and reliable for clinical use, and can be used or conventional adherent and suspension cells.

The utility model relates to a kind of cell vitrification freezing automatic systems, including workbench;Including several injection pumps, the injection pump is installed on workbench, movable piston rod is provided in the injection pump, and the piston rod upper end is connected with driving assembly;Including fluid collecting valve, the fluid collecting valve is installed on workbench, and the fluid collecting valve is connected with the liquid outlet end of injection pump;Including frozen macaroni, the frozen macaroni and fluid collecting valve are connected;Including temperature control board, the temperature control board is installed on workbench, the utility model is advantageous in that system automatically controls the loading and unloading of cryoprotectant, to controllable mode adjustment exchange in oocyte water and cryoprotectant, reduce the pressure or organelle and intracellular membrane system damage of cell structure of osmotic pressure shock to cell, improve the survival rate of cell.

The invention relates to a germplasm cryopreservation method suitable for bait microalgae, and belongs to the field of algae preservation, and the method comprises the following steps: carrying out low-temperature domestication on microalgae in a logarithmic phase, and centrifugally collecting algae mud; mixing the algae mud with a protective solution for pretreatment; after pretreatment, mixing with a cryoprotectant with specific composition, carrying out gradient cooling, and storing at-80 DEG C; during thawing, the cryopreservation tube is quickly thawed in a water bath, and the cryoprotectant is subjected to gradient elution through a sucrose solution. The method successfully realizes long-term stable preservation of various bait microalgae at-80 DEG C by optimizing a cryoprotectant combination and optimizing key parameters such as a protective solution, balance time and thawing temperature, has high survival rate and stable physiological traits after resuscitation, and is low in cost, convenient to operate and suitable for industrial production. And a new technical scheme is provided for protection and utilization of microalgae germplasm resources.

The present invention is drawn to a novel buffer composition comprising a nuclease quenching agent, a stabilizer, a salt, and a cryoprotectant. The present invention is also drawn to a process of producing the composition of the present invention and its utility. The composition of the present invention supports viability of aerobic and anaerobic microbes for extended periods over significant range of temperatures, without the presence of any significant amount of degradants of the constituents of the samples such as biological macromolecules and DNA. The present invention also relates to an economically viable and wide range applications for storage of biological and environmental samples containing microbes.

The invention relates to the technical field of poultry breed conservation biology, and discloses a cryopreservation kit for chicken semen and a use method of the cryopreservation kit. The invention relates to a cryopreservation kit for chicken semen. The cryopreservation kit comprises a cryopreservation diluent, a cryoprotectant, an insemination diluent, a DMEM (dulbecco's modified eagle medium) and a frozen semen tube. The cryopreservation diluent in the kit provided by the invention can provide a better living environment for chicken sperms, and the cryoprotectant in the kit can successfully perform cryopreservation on the chicken sperms; when the kit provided by the invention is used for freezing and storing chicken semen, the unfrozen chicken semen is used for artificial insemination, the fertilization rate can reach 70% or above, and the requirement for breed conservation of chicken genetic resources is met.

The invention discloses a vitrification device for realizing concentration curve change, and relates to the technical field of biological sample low-temperature preservation, the vitrification device comprises an operation table, an intelligent control module and a multifunctional interaction module; the operating table comprises a gradient refrigerating fluid mechanism, a dynamic temperature control module, a multi-channel micro-fluidic chip, an image acquisition device and a working table, and the gradient refrigerating fluid mechanism is used for storing cryoprotectants with different concentrations, conveying the cryoprotectants to the multi-channel micro-fluidic chip, dynamically mixing the cryoprotectants according to a preset proportion and wrapping a biological sample; the dynamic temperature control module is used for adjusting the temperature in the vitrification process, and the image acquisition device is used for acquiring a morphological change image of a sample; the intelligent control module is used for controlling the filling flow, the mixing proportion and the cooling of the cryoprotectant and sending an alarm reminding signal to the multifunctional interaction module. According to the application, the concentration and temperature change of the cryoprotectant in the freezing process can be accurately controlled, efficient vitrification is realized, and the damage of a sample in the freezing process is effectively reduced.

An automated processing system for vitrification-cryopreservation comprising a control mechanism for fixing and controlling the displacement of a cryo-carrier holding thereon samples to undergo vitrification-cryopreservation. The control mechanism achieves operations such as flipping, lifting, and translation of the cryo-carrier, thereby immersing or detaching the samples to be cryopreserved on the cryo-carrier from the cryoprotectant. The control mechanism can also transport the cryo-carrier to a cryogenic device and immerse the samples to be cryopreserved in the refrigerant for freezing.

Colorimetric methods and kits for determining anti-icing effects of testing materials are presented. In embodiments, a method includes: mixing a testing material with a dithiolate-based ligand-grafted gold nanoparticle (AuNP) probe in solution, thereby generating a test sample; chilling the test sample at a predetermined temperature for a period of time; subsequent to chilling the test sample, detecting a color of the test sample; and determining anti-icing effects of the testing material based on the color of the test sample. Further, bidentate compounds are presented. The compounds have a bidentate binding group: thioctic acid (TA) with disulfide or its reduced form of dihydrolipoic acid (DHLA) with dithiol. In embodiments, the compounds form part of the dithiolate-based ligand- grafted gold nanoparticle (AuNP).

The purpose of the present invention is to obtain a cell suspension with a low concentration of cryoprotectant. This method of preserving cells used comprises the steps of (a) enriching cells from a cell suspension containing the cells and a cryoprotective solution to generate an enriched fraction, and (b) freezing the enriched fraction to prepare a frozen material. It is also possible to use a method of producing a cell suspension, comprising the steps of (a) enriching cells from a cell suspension containing the cells and a cryoprotective solution to generate an enriched fraction, (b) freezing the enriched fraction to prepare a frozen material, (c) thawing the frozen material to prepare a thawed material, and (d) mixing the thawed material and a solution to produce a cell suspension.

The present invention discloses a device for cryopreservation or thawing and recovery of biological tissues. The device according to an embodiment of the present invention includes: a biochip, which includes a solution inlet and a solution outlet; a carrier, which has a first groove, and the biological tissue is carried in the first groove; wherein, the carrier is closely attached below the biochip, and a microchannel passing through the first groove is formed between the biochip and the carrier, and only the solution can pass through the microchannel. Through the present invention, the delivery or removal of cryoprotectant can be carried out in an orderly manner, effectively improving the efficiency of embryo / egg cryopreservation or thawing and recovery operations.

Provided is a nanoparticle composition comprising a compound represented by general formula (1), or an ionized form thereof, where R1, R2 and R4 to R8 are each independently H, an optionally substituted alkyl group, an optionally substituted alkenyl group, or an optionally substituted alkynyl group, R3 is an optionally substituted alkylene group, an optionally substituted alkenylene group, or an optionally substituted alkynyl group, and R4 is an optionally substituted alkyl group, an optionally substituted alkenylene group, or an optionally substituted alkynyl group. And R9 and R10 are each independently a hydrophobic tail or contain at least one of the groups as defined above for R4 to R8; therapeutic, prophylactic and / or biological agents encapsulated with a compound of general formula (1) to form nanoparticles; and a cryoprotectant. Methods of making the nanoparticle compositions are also provided.

The invention provides a bacteriophage freeze-drying protective agent, a bacteriophage freeze-drying powder, a preparation method and application, and belongs to the technical field of biological medicine preparations. The freeze-drying protective agent comprises the following components: a cryoprotectant, an antioxidant, an acid-base regulator and a filler. The freeze-drying protective agent disclosed by the invention is reasonably compounded, and the freeze-drying protective agent, the antioxidant, the pH regulator and the filler have a good protective effect on the bacteriophage through a synergistic effect, so that the inactivation rate or the death rate of the bacteriophage in a freeze-drying process can be effectively reduced, and the biological activity and the titer of the bacteriophage are improved. Moreover, the freeze-drying protective agent disclosed by the invention is relatively long in protective effect acting time, can improve the stability of the freeze-dried powder of the bacteriophage, improves the transportation and storage convenience of the freeze-dried powder of the bacteriophage, and effectively solves the problem that the freeze-dried powder of the bacteriophage is difficult to transport and store for a long time in practical application.