118 results about "Cryoprotectant" patented technology

The present disclosure includes hyperbranched polyether polyols, such as hyperbranched poly(3-(oxiran-2-ylmethoxy) propane-1,2-diol) (HPOD), methods for their preparation and uses thereof, for example, as a cryoprotectant, lyoprotectant or stabilizer.

The invention discloses a cryoprotectant which comprises glycerol, skimmed milk powder, mannitol, trehalose, vitamin C, fructo-oligosaccharide, inulin and sterile water. By optimizing the components of the cryoprotectant, the activity of the coprophilous fungi can be effectively protected, the survival rate of the intestinal coprophilous fungi in the freezing process is remarkably increased, all the used components are food-grade edible components and have a wide application range and economic effects, the invention further discloses a coprophilous fungi freezing preservation method adopting the cryoprotectant, and the cryoprotectant has good application prospects. The method is simple, easy to operate and suitable for large-scale production and application.

The invention belongs to the field of marine food processing, and provides a marine food containing yellow croaker and a preparation method thereof.The marine food containing yellow croaker is prepared by adopting collaborative design of a minced yellow croaker protein-polysaccharide composite nano colloid powder intermediate and low-molecular fish collagen, through microbial transglutaminase cross-linking and cryoprotectant compounding regulation and control, and the marine food containing yellow croaker and the preparation method of the marine food containing yellow croaker are obtained. A minced fillet protein continuous phase network structure with uniformly dispersed nano-particles is constructed, the gel strength is 900-1300 g.cm, the gel strength retention rate is not lower than 80% after 6 times of freezing and thawing cycles are frozen for 3-5 times, the unfreezing drip loss is not higher than 8 wt%, the hardness is 1500-2500 g, and the taste characteristics of high strength and good elasticity are obtained. The technical problems that high gel strength and freeze-thaw stability of a traditional surimi product are difficult to consider, the taste is worse easily due to improvement of mechanical properties, nano-particles are difficult to uniformly disperse in a high-solid-content system and the like are solved, and the surimi product has wide application value in the fields of surimi products, quick-frozen prepared foods and marine functional foods.

The invention relates to the technical field of organ transplantation, and discloses organ preserving fluid, application and organ dynamic supercooling perfusion equipment. The organ preserving fluid is prepared from ribose, glucose, mannitol, lidocaine, adenosine, a high-molecular polymer, L-proline, trehalose, inorganic salt and organic salt. The high-molecular polymer comprises one of hydroxyethyl starch, polyethylene glycol and albumin. According to the organ preserving fluid disclosed by the invention, the freezing point of the perfusate is reduced through the micromolecular cryoprotectant, and meanwhile, the micromolecular cryoprotectant and the high-molecular polymer jointly inhibit formation and growth of ice crystals; the problem of liquid preservation at the temperature below zero is solved from the two dimensions of physics (reducing the freezing point and inhibiting ice crystals) and biology (maintaining cell viability), and feasibility is provided for long-time low-temperature transfer and transplantation of organs. The preserving fluid can be used for static preservation or perfusion preservation of various organs such as kidney, liver, heart, pancreas, lung, small intestine, skin and the like.

The present invention relates to compositions for storing microbiota, in particular microbiota compositions comprising inulin and maltodextrin as cryoprotectants / lyoprotectants. The present invention also relates to dosage forms and methods of treating disorders and diseases by administering the composition to a patient in need thereof.

The present disclosure provides a liquid cryoprotectant having a lactam moiety structure of wherein the lactam having at least one hydroxyl functional group; wherein Y is an alkylene or alkenylene group comprising 2 to 50 carbon atoms; wherein 2 to 4 carbon atoms reside in the lactam ring between the I group and the II group; Q is selected from the group consisting of functionalized and unfunctionalized alkylenes of linear, branched, or cyclic structure, rylenes, and combinations thereof, wherein the aforementioned groups may or may not contain oxygen atom(s); and R is selected from the group consisting of hydrogen and functionalized and unfunctionalized alkyl, cycloalkyl, alkenyl, and aryl groups, wherein the alkyl, cycloalkyl, alkenyl, and aryl groups may or may not contain heteroatoms. The present disclosure also provides a composition for cryopreservation having a lactam moiety structure.

The invention discloses a novel antifreeze polypeptide with a clear structure and excellent performance and a protection method of cryopreserved cells. The core structure of the polypeptide is formed by covalently connecting two functional modules, the N end is a unit with ice crystal binding performance, the C end is a super-hydrophilic unit, and the two functional modules are bridged through a flexible linker. Experimental verification shows that the synthetic polypeptide shows excellent anti-freezing activity, can significantly inhibit nucleation, growth and recrystallization processes of ice crystals, and has particularly outstanding ice recrystallization inhibition activity. The novel antifreeze polypeptide shows excellent biocompatibility, and is suitable for low-temperature cryopreservation of cells sensitive to freezing damage and other living microbial preparations. Results show that the polypeptide can greatly improve the freeze-drying survival rate of cells and the activity of the cells after recovery, is non-toxic to the cells, and shows the potential of replacing a traditional cryoprotectant. Therefore, the method has wide application prospects in food industry, biological medicine, agricultural breeding and low-temperature preservation technologies of biological sample libraries.

The invention relates to the technical field of cell biology, in particular to an iNKT cell cryopreservation solution and a method for cryopreserving iNKT cells by using the cryopreservation solution. The invention provides an immune cell cryopreservation solution. The immune cell cryopreservation solution comprises human serum albumin, a basic low-temperature protection solution and an optional human platelet lysate. The immune cell freezing medium does not contain animal-derived components except for human bodies, and has the advantages of simple components and good stability. According to the cryopreservation liquid disclosed by the invention, through the combined action of the basic low-temperature protection liquid (such as CryoStor10 (CS10)) and the human serum albumin, the motility rate after cell recovery is improved, and the damage to immune cells (such as iNKT) in the cryopreservation process is reduced. After a small amount of human platelet lysate is further added, the survival rate of cell resuscitation and the amplification capacity can be further improved.

A microfluidic device that includes a trap configured to retain an oocyte or fertilized species, a plurality of microchannels in fluid communication with the trap, and a plurality of valves, where each valve is configured to control fluid flow through a corresponding set of one or more of the plurality of microchannels. The microfluidic device further includes a plurality of fluidic timer circuits each configured to produce a corresponding first time-dependent fluid pressure signal, and a fluidic logic gate circuit configured to receive the first time-dependent pressure signals and to produce a plurality of second time-dependent fluid pressure signals, where each second time-dependent fluid pressure signal controls operation of a corresponding one of the valves.

The present disclosure is concerned with peptides comprising alanine residues and glutamic acid residues in a particular ratio (e.g., from about 1 : 1 to about 4; I ). wherein the peptide has a minimum chain length, such as, for example a chain length of at least 15 or at least 20 amino acid residues. The disclosed peptides beneficially inhibit ice crystal formation, and, therefore, offer utility in a wide range of applications, including, but not limited to biomedical cryopreservation. food technology, agriculture, cosmetics, and building materials. Thus, the disclosed peptides can be formulated into a composition (e.g.. a cryoprotectant composition, an agricultural formulation, a cosmetic composition) or a food product, or, alternatively, can be attached or coated onto a surface for use in structural applications. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention.

Large volume cellular material may be preserved by combining the cellular material with a cryoprotectant formulation / medium / solution containing at least one mNP and then subjecting the cellular material to a vitrification preservation protocol including nanowarming. This preservation method is particularly effective for cartilage tissues.

The utility model relates to a kind of cell vitrification freezing automatic systems, including workbench;Including several injection pumps, the injection pump is installed on workbench, movable piston rod is provided in the injection pump, and the piston rod upper end is connected with driving assembly;Including fluid collecting valve, the fluid collecting valve is installed on workbench, and the fluid collecting valve is connected with the liquid outlet end of injection pump;Including frozen macaroni, the frozen macaroni and fluid collecting valve are connected;Including temperature control board, the temperature control board is installed on workbench, the utility model is advantageous in that system automatically controls the loading and unloading of cryoprotectant, to controllable mode adjustment exchange in oocyte water and cryoprotectant, reduce the pressure or organelle and intracellular membrane system damage of cell structure of osmotic pressure shock to cell, improve the survival rate of cell.

The invention relates to a germplasm cryopreservation method suitable for bait microalgae, and belongs to the field of algae preservation, and the method comprises the following steps: carrying out low-temperature domestication on microalgae in a logarithmic phase, and centrifugally collecting algae mud; mixing the algae mud with a protective solution for pretreatment; after pretreatment, mixing with a cryoprotectant with specific composition, carrying out gradient cooling, and storing at-80 DEG C; during thawing, the cryopreservation tube is quickly thawed in a water bath, and the cryoprotectant is subjected to gradient elution through a sucrose solution. The method successfully realizes long-term stable preservation of various bait microalgae at-80 DEG C by optimizing a cryoprotectant combination and optimizing key parameters such as a protective solution, balance time and thawing temperature, has high survival rate and stable physiological traits after resuscitation, and is low in cost, convenient to operate and suitable for industrial production. And a new technical scheme is provided for protection and utilization of microalgae germplasm resources.

The invention relates to the technical field of poultry breed conservation biology, and discloses a cryopreservation kit for chicken semen and a use method of the cryopreservation kit. The invention relates to a cryopreservation kit for chicken semen. The cryopreservation kit comprises a cryopreservation diluent, a cryoprotectant, an insemination diluent, a DMEM (dulbecco's modified eagle medium) and a frozen semen tube. The cryopreservation diluent in the kit provided by the invention can provide a better living environment for chicken sperms, and the cryoprotectant in the kit can successfully perform cryopreservation on the chicken sperms; when the kit provided by the invention is used for freezing and storing chicken semen, the unfrozen chicken semen is used for artificial insemination, the fertilization rate can reach 70% or above, and the requirement for breed conservation of chicken genetic resources is met.

The invention discloses a vitrification device for realizing concentration curve change, and relates to the technical field of biological sample low-temperature preservation, the vitrification device comprises an operation table, an intelligent control module and a multifunctional interaction module; the operating table comprises a gradient refrigerating fluid mechanism, a dynamic temperature control module, a multi-channel micro-fluidic chip, an image acquisition device and a working table, and the gradient refrigerating fluid mechanism is used for storing cryoprotectants with different concentrations, conveying the cryoprotectants to the multi-channel micro-fluidic chip, dynamically mixing the cryoprotectants according to a preset proportion and wrapping a biological sample; the dynamic temperature control module is used for adjusting the temperature in the vitrification process, and the image acquisition device is used for acquiring a morphological change image of a sample; the intelligent control module is used for controlling the filling flow, the mixing proportion and the cooling of the cryoprotectant and sending an alarm reminding signal to the multifunctional interaction module. According to the application, the concentration and temperature change of the cryoprotectant in the freezing process can be accurately controlled, efficient vitrification is realized, and the damage of a sample in the freezing process is effectively reduced.

An automated processing system for vitrification-cryopreservation comprising a control mechanism for fixing and controlling the displacement of a cryo-carrier holding thereon samples to undergo vitrification-cryopreservation. The control mechanism achieves operations such as flipping, lifting, and translation of the cryo-carrier, thereby immersing or detaching the samples to be cryopreserved on the cryo-carrier from the cryoprotectant. The control mechanism can also transport the cryo-carrier to a cryogenic device and immerse the samples to be cryopreserved in the refrigerant for freezing.

The purpose of the present invention is to obtain a cell suspension with a low concentration of cryoprotectant. This method of preserving cells used comprises the steps of (a) enriching cells from a cell suspension containing the cells and a cryoprotective solution to generate an enriched fraction, and (b) freezing the enriched fraction to prepare a frozen material. It is also possible to use a method of producing a cell suspension, comprising the steps of (a) enriching cells from a cell suspension containing the cells and a cryoprotective solution to generate an enriched fraction, (b) freezing the enriched fraction to prepare a frozen material, (c) thawing the frozen material to prepare a thawed material, and (d) mixing the thawed material and a solution to produce a cell suspension.

Provided is a nanoparticle composition comprising a compound represented by general formula (1), or an ionized form thereof, where R1, R2 and R4 to R8 are each independently H, an optionally substituted alkyl group, an optionally substituted alkenyl group, or an optionally substituted alkynyl group, R3 is an optionally substituted alkylene group, an optionally substituted alkenylene group, or an optionally substituted alkynyl group, and R4 is an optionally substituted alkyl group, an optionally substituted alkenylene group, or an optionally substituted alkynyl group. And R9 and R10 are each independently a hydrophobic tail or contain at least one of the groups as defined above for R4 to R8; therapeutic, prophylactic and / or biological agents encapsulated with a compound of general formula (1) to form nanoparticles; and a cryoprotectant. Methods of making the nanoparticle compositions are also provided.

The invention relates to the technical field of biomedical detection, and discloses an umbilical cord blood unfreezing reference specimen cell counting and flow cytometry dilution method which comprises the following steps: S1, preparing an unfreezing solution which is composed of HES, AB plasma and PBS, and placing the unfreezing solution at room temperature for later use; s2, taking the unfrozen umbilical cord blood unfreezing reference specimen, adding an unfreezing solution into the umbilical cord blood, firstly gently and uniformly mixing, then standing at room temperature, then continuing to add the unfreezing solution, gently and uniformly mixing again, then standing at room temperature, and stabilizing; s3, taking the diluted umbilical cord blood, and carrying out cell counting by adopting a counting instrument; and S4, taking the diluted umbilical cord blood again, and dyeing. The unfreezing solution composed of HES, AB plasma and PBS synergistically regulates osmotic pressure, provides cell nutrition, stabilizes the pH value, reduces the damage of the cryoprotectant to cord blood cells, maintains the cell activity, and improves the problem of result deviation caused by direct detection of cell damage in the prior art.

The invention belongs to the technical field of biology, and relates to an exosome active cryoprotectant and a preparation method thereof. The exosome activity freezing protection liquid is prepared from trehalose, proline, hydroxypropyl methyl cellulose, poloxamer 188, polyvinyl alcohol, propylene glycol and a disodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution. According to the exosome preserving fluid and the exosome preserving method provided by the invention, the stability of the exosome can be effectively improved, the preservation time of the exosome is prolonged, and meanwhile, the biological activity of the exosome is maintained.

The application provides a method for predicting the ice inhibition performance of nanometer silicon dioxide based on molecular dynamics simulation. The application comprises the following steps: establishing a functionalized nanometer silicon dioxide model, and establishing an ice-containing water box model according to the size of the functionalized nanometer silicon dioxide model; assembling the nanometer silicon dioxide model and the water box model; selecting a suitable force field for the constructed model and performing energy minimization optimization. Molecular dynamics simulation calculation is performed on the silicon dioxide-ice-water model after energy minimization, and analysis is performed on the model after calculation. The root mean square deviation, root mean square fluctuation and binding energy are analyzed, and the number of hydrogen bonds is counted. Based on the analysis data, the feasibility and effect of the material for inhibiting ice are judged. The application constructs different functionalized materials and evaluates their behavior in the ice-water system, thereby greatly reducing the application cost, saving experimental resources, and guiding the development of a freezing protectant based on nanometer materials.

The invention provides a larch callus ultralow-temperature storage and rapid growth recovery method, and belongs to the technical field of forest tree tissue culture breeding. The method comprises the following steps: (1) pretreating and pre-culturing calluses: taking the larch calluses, and inoculating the calluses into a pretreatment culture medium for culturing; 2) pre-culture treatment with a cryoprotectant: transferring the pre-cultured calluses into a composite cryoprotectant for soaking treatment; (3) performing segmented cryopreservation: putting the callus subjected to pre-culture treatment by a cryoprotectant into a sterile cryopreservation tube, adding a composite cryoprotectant, and performing segmented cryopreservation; 4) gradient heating unfreezing: taking out a cryopreservation tube for cryopreservation, carrying out gradient heating unfreezing, and after unfreezing, transferring the callus to a BM2 washing culture medium for washing, and 5) rapid recovery culture: inoculating the washed callus to a recovery culture medium for culture.The method provided by the invention can effectively avoid damage to the callus caused by cryopreservation.

A lyophilized composition capable of encapsulating any nucleic acid with high efficiency and easily is provided. A lyophilized composition of lipid nanoparticles not containing a nucleic acid but containing an ionic lipid, a sterol, a PEG lipid, an acidic buffer component that shows a buffering action at pH 1-6, and a cryoprotectant, wherein a weight ratio of the cryoprotectant and a total lipid is 10:1-1000:1.

The invention relates to the technical field of fermentation of medicinal material production, and discloses a preservation method of a pseudo-ginseng cell strain, which comprises the following steps: acquiring a working cell strain; obtaining a liquid culture medium; carrying out mixed culture on the working cell strain and the liquid culture medium, and dehydrating; and soaking the dehydrated biomass in a cryoprotectant, and then carrying out cryopreservation or refrigerated preservation. Through cryopreservation or refrigerated preservation of the mixed solution, the preserved working cell strain can be adopted for fermentation in real time according to the requirements of a user, the richness of the strain can be guaranteed, the preparation period of the seed solution is saved, multi-batch fermentation can be realized, and the yield is stable.

The invention relates to an extracellular vesicle-frozen gel scaffold as well as an integrated preparation method and application thereof, and belongs to the technical field of biotechnology and tissue engineering materials. The problems that in the prior art, extracellular vesicles are prone to being damaged by low temperature in the frozen gel preparation process, a traditional cryoprotectant is not compatible with a stent process, and burst release and action time is short in a vesicle physical adsorption method are solved. The method comprises the following steps: mixing extracellular vesicles with a trehalose solution, and standing to obtain a vesicle-trehalose mixed solution; mixing with the support material prepolymer solution, and adding a photoinitiator to form a composite precursor solution; freezing the solution, and performing cross-linking solidification to form a three-dimensional porous gel scaffold; and unfreezing and washing to obtain the stent. The trehalose is introduced as a cryoprotectant, vesicle activity protection and stent structure forming are synchronously achieved in low-temperature forming, and the prepared stent can achieve controllable slow release of vesicles and has good application prospects in the field of tissue repair and regeneration.

A bioink for cryobioprinting is described. The bioink comprises a decellularized extracellular matrix (dECM)-based hydrogel and a saccharide cryoprotectant. A method of making a 3D-printed biomaterial is also described, which includes depositing a bioink comprising a dECM-based hydrogel, cells, and a saccharide cryoprotectant from a 3D printer onto a freezing plate to form a frozen bioink filament; depositing additional bioink from a 3D printer in contact with a previously placed frozen bioink filament to form a 3D-printed biomaterial comprising a plurality of frozen bioink filaments; and removing the 3D-printed biomaterial from the freezing plate. 3D-printed biomaterials made according to the method are also described.

The invention provides a screening method of a low-temperature protective agent, which comprises the following steps: constructing a solution system containing a plurality of single-component low-temperature protective agents, respectively carrying out molecular dynamics simulation to calculate a plurality of microscopic characteristics and target macroscopic characteristics of each solution, and constructing a total microscopic characteristic data set and each target macroscopic characteristic data set; based on clustering and importance analysis and in combination with the corresponding target macroscopic feature data set and machine learning, determining evaluation features for each target macroscopic feature from the total microscopic feature data set, and obtaining a prediction model of each target macroscopic feature at the same time; calculating evaluation characteristics of the low-temperature protective agent to be screened, and respectively inputting the evaluation characteristics into the corresponding target macroscopic characteristic prediction models to obtain corresponding prediction values; predicting the toxicity of the low-temperature protective agent to be screened; and taking the to-be-screened low-temperature protective agent of which all the target macroscopic feature predicted values and toxicity predicted values meet the requirements as a screening result. The method has the characteristics of rapidness, accuracy and low cost.

The present disclosure provides a method of identifying an intracellular cryoprotectant expressed by a cell, the method comprising: (a) using a cell that survives in freezing and thawing, (b) modifying the used cell, (c) freezing and thawing the modified cell, and (d) obtaining a modified cell that survives after step (c), an intracellular cryoprotectant expressed by the cell is thus identified, as well as an intracellular cryoprotectant obtained by or which can be obtained by the method.

The invention discloses a cryopreservation method of haliotis diversicolor sperms. The method comprises the following steps: collecting the sperms, testing the initial activity of the sperms, preparing sperm freezing liquid, balancing and freezing the sperm freezing liquid, unfreezing the frozen sperms, and testing the activity after unfreezing. The collected male haliotis discus hannai sperms with the initial activity higher than 90% and a cryoprotectant are mixed according to the volume ratio of 1: 3, and sperm refrigerating fluid is obtained; transferring the sperm freezing liquid into straw, balancing, freezing, and soaking in liquid nitrogen for preservation to obtain frozen sperms; putting the straw filled with the frozen sperms into a water bath kettle, and unfreezing to obtain unfrozen sperms; finally, the straw containing the thawed sperms is cut open, the thawed sperms are transferred to a centrifugal tube, activity testing is carried out, and the activity and quality of the haliotis discus hannai sperms after cryopreservation are remarkably improved through the method.

A lyophilized composition capable of encapsulating any nucleic acid with high efficiency and easily is provided. A lyophilized composition of lipid nanoparticles not containing a nucleic acid but containing an ionic lipid, a sterol, a PEG lipid, an acidic buffer component that shows a buffering action at pH 1-6, and a cryoprotectant, wherein a weight ratio of the cryoprotectant and a total lipid is 10:1-1000:1.