67 results about "Cryoprotectant" patented technology

The invention belongs to the field of marine food processing, and provides a marine food containing yellow croaker and a preparation method thereof.The marine food containing yellow croaker is prepared by adopting collaborative design of a minced yellow croaker protein-polysaccharide composite nano colloid powder intermediate and low-molecular fish collagen, through microbial transglutaminase cross-linking and cryoprotectant compounding regulation and control, and the marine food containing yellow croaker and the preparation method of the marine food containing yellow croaker are obtained. A minced fillet protein continuous phase network structure with uniformly dispersed nano-particles is constructed, the gel strength is 900-1300 g.cm, the gel strength retention rate is not lower than 80% after 6 times of freezing and thawing cycles are frozen for 3-5 times, the unfreezing drip loss is not higher than 8 wt%, the hardness is 1500-2500 g, and the taste characteristics of high strength and good elasticity are obtained. The technical problems that high gel strength and freeze-thaw stability of a traditional surimi product are difficult to consider, the taste is worse easily due to improvement of mechanical properties, nano-particles are difficult to uniformly disperse in a high-solid-content system and the like are solved, and the surimi product has wide application value in the fields of surimi products, quick-frozen prepared foods and marine functional foods.

The present disclosure provides a liquid cryoprotectant having a lactam moiety structure of wherein the lactam having at least one hydroxyl functional group; wherein Y is an alkylene or alkenylene group comprising 2 to 50 carbon atoms; wherein 2 to 4 carbon atoms reside in the lactam ring between the I group and the II group; Q is selected from the group consisting of functionalized and unfunctionalized alkylenes of linear, branched, or cyclic structure, rylenes, and combinations thereof, wherein the aforementioned groups may or may not contain oxygen atom(s); and R is selected from the group consisting of hydrogen and functionalized and unfunctionalized alkyl, cycloalkyl, alkenyl, and aryl groups, wherein the alkyl, cycloalkyl, alkenyl, and aryl groups may or may not contain heteroatoms. The present disclosure also provides a composition for cryopreservation having a lactam moiety structure.

The invention discloses a novel antifreeze polypeptide with a clear structure and excellent performance and a protection method of cryopreserved cells. The core structure of the polypeptide is formed by covalently connecting two functional modules, the N end is a unit with ice crystal binding performance, the C end is a super-hydrophilic unit, and the two functional modules are bridged through a flexible linker. Experimental verification shows that the synthetic polypeptide shows excellent anti-freezing activity, can significantly inhibit nucleation, growth and recrystallization processes of ice crystals, and has particularly outstanding ice recrystallization inhibition activity. The novel antifreeze polypeptide shows excellent biocompatibility, and is suitable for low-temperature cryopreservation of cells sensitive to freezing damage and other living microbial preparations. Results show that the polypeptide can greatly improve the freeze-drying survival rate of cells and the activity of the cells after recovery, is non-toxic to the cells, and shows the potential of replacing a traditional cryoprotectant. Therefore, the method has wide application prospects in food industry, biological medicine, agricultural breeding and low-temperature preservation technologies of biological sample libraries.

The invention relates to the technical field of cell biology, in particular to an iNKT cell cryopreservation solution and a method for cryopreserving iNKT cells by using the cryopreservation solution. The invention provides an immune cell cryopreservation solution. The immune cell cryopreservation solution comprises human serum albumin, a basic low-temperature protection solution and an optional human platelet lysate. The immune cell freezing medium does not contain animal-derived components except for human bodies, and has the advantages of simple components and good stability. According to the cryopreservation liquid disclosed by the invention, through the combined action of the basic low-temperature protection liquid (such as CryoStor10 (CS10)) and the human serum albumin, the motility rate after cell recovery is improved, and the damage to immune cells (such as iNKT) in the cryopreservation process is reduced. After a small amount of human platelet lysate is further added, the survival rate of cell resuscitation and the amplification capacity can be further improved.

A microfluidic device that includes a trap configured to retain an oocyte or fertilized species, a plurality of microchannels in fluid communication with the trap, and a plurality of valves, where each valve is configured to control fluid flow through a corresponding set of one or more of the plurality of microchannels. The microfluidic device further includes a plurality of fluidic timer circuits each configured to produce a corresponding first time-dependent fluid pressure signal, and a fluidic logic gate circuit configured to receive the first time-dependent pressure signals and to produce a plurality of second time-dependent fluid pressure signals, where each second time-dependent fluid pressure signal controls operation of a corresponding one of the valves.

The utility model relates to a kind of cell vitrification freezing automatic systems, including workbench;Including several injection pumps, the injection pump is installed on workbench, movable piston rod is provided in the injection pump, and the piston rod upper end is connected with driving assembly;Including fluid collecting valve, the fluid collecting valve is installed on workbench, and the fluid collecting valve is connected with the liquid outlet end of injection pump;Including frozen macaroni, the frozen macaroni and fluid collecting valve are connected;Including temperature control board, the temperature control board is installed on workbench, the utility model is advantageous in that system automatically controls the loading and unloading of cryoprotectant, to controllable mode adjustment exchange in oocyte water and cryoprotectant, reduce the pressure or organelle and intracellular membrane system damage of cell structure of osmotic pressure shock to cell, improve the survival rate of cell.

The invention relates to a germplasm cryopreservation method suitable for bait microalgae, and belongs to the field of algae preservation, and the method comprises the following steps: carrying out low-temperature domestication on microalgae in a logarithmic phase, and centrifugally collecting algae mud; mixing the algae mud with a protective solution for pretreatment; after pretreatment, mixing with a cryoprotectant with specific composition, carrying out gradient cooling, and storing at-80 DEG C; during thawing, the cryopreservation tube is quickly thawed in a water bath, and the cryoprotectant is subjected to gradient elution through a sucrose solution. The method successfully realizes long-term stable preservation of various bait microalgae at-80 DEG C by optimizing a cryoprotectant combination and optimizing key parameters such as a protective solution, balance time and thawing temperature, has high survival rate and stable physiological traits after resuscitation, and is low in cost, convenient to operate and suitable for industrial production. And a new technical scheme is provided for protection and utilization of microalgae germplasm resources.

An automated processing system for vitrification-cryopreservation comprising a control mechanism for fixing and controlling the displacement of a cryo-carrier holding thereon samples to undergo vitrification-cryopreservation. The control mechanism achieves operations such as flipping, lifting, and translation of the cryo-carrier, thereby immersing or detaching the samples to be cryopreserved on the cryo-carrier from the cryoprotectant. The control mechanism can also transport the cryo-carrier to a cryogenic device and immerse the samples to be cryopreserved in the refrigerant for freezing.

The purpose of the present invention is to obtain a cell suspension with a low concentration of cryoprotectant. This method of preserving cells used comprises the steps of (a) enriching cells from a cell suspension containing the cells and a cryoprotective solution to generate an enriched fraction, and (b) freezing the enriched fraction to prepare a frozen material. It is also possible to use a method of producing a cell suspension, comprising the steps of (a) enriching cells from a cell suspension containing the cells and a cryoprotective solution to generate an enriched fraction, (b) freezing the enriched fraction to prepare a frozen material, (c) thawing the frozen material to prepare a thawed material, and (d) mixing the thawed material and a solution to produce a cell suspension.

Provided is a nanoparticle composition comprising a compound represented by general formula (1), or an ionized form thereof, where R1, R2 and R4 to R8 are each independently H, an optionally substituted alkyl group, an optionally substituted alkenyl group, or an optionally substituted alkynyl group, R3 is an optionally substituted alkylene group, an optionally substituted alkenylene group, or an optionally substituted alkynyl group, and R4 is an optionally substituted alkyl group, an optionally substituted alkenylene group, or an optionally substituted alkynyl group. And R9 and R10 are each independently a hydrophobic tail or contain at least one of the groups as defined above for R4 to R8; therapeutic, prophylactic and / or biological agents encapsulated with a compound of general formula (1) to form nanoparticles; and a cryoprotectant. Methods of making the nanoparticle compositions are also provided.

The invention relates to the technical field of biomedical detection, and discloses an umbilical cord blood unfreezing reference specimen cell counting and flow cytometry dilution method which comprises the following steps: S1, preparing an unfreezing solution which is composed of HES, AB plasma and PBS, and placing the unfreezing solution at room temperature for later use; s2, taking the unfrozen umbilical cord blood unfreezing reference specimen, adding an unfreezing solution into the umbilical cord blood, firstly gently and uniformly mixing, then standing at room temperature, then continuing to add the unfreezing solution, gently and uniformly mixing again, then standing at room temperature, and stabilizing; s3, taking the diluted umbilical cord blood, and carrying out cell counting by adopting a counting instrument; and S4, taking the diluted umbilical cord blood again, and dyeing. The unfreezing solution composed of HES, AB plasma and PBS synergistically regulates osmotic pressure, provides cell nutrition, stabilizes the pH value, reduces the damage of the cryoprotectant to cord blood cells, maintains the cell activity, and improves the problem of result deviation caused by direct detection of cell damage in the prior art.

The invention belongs to the technical field of biology, and relates to an exosome active cryoprotectant and a preparation method thereof. The exosome activity freezing protection liquid is prepared from trehalose, proline, hydroxypropyl methyl cellulose, poloxamer 188, polyvinyl alcohol, propylene glycol and a disodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution. According to the exosome preserving fluid and the exosome preserving method provided by the invention, the stability of the exosome can be effectively improved, the preservation time of the exosome is prolonged, and meanwhile, the biological activity of the exosome is maintained.

The application provides a method for predicting the ice inhibition performance of nanometer silicon dioxide based on molecular dynamics simulation. The application comprises the following steps: establishing a functionalized nanometer silicon dioxide model, and establishing an ice-containing water box model according to the size of the functionalized nanometer silicon dioxide model; assembling the nanometer silicon dioxide model and the water box model; selecting a suitable force field for the constructed model and performing energy minimization optimization. Molecular dynamics simulation calculation is performed on the silicon dioxide-ice-water model after energy minimization, and analysis is performed on the model after calculation. The root mean square deviation, root mean square fluctuation and binding energy are analyzed, and the number of hydrogen bonds is counted. Based on the analysis data, the feasibility and effect of the material for inhibiting ice are judged. The application constructs different functionalized materials and evaluates their behavior in the ice-water system, thereby greatly reducing the application cost, saving experimental resources, and guiding the development of a freezing protectant based on nanometer materials.

The invention provides a larch callus ultralow-temperature storage and rapid growth recovery method, and belongs to the technical field of forest tree tissue culture breeding. The method comprises the following steps: (1) pretreating and pre-culturing calluses: taking the larch calluses, and inoculating the calluses into a pretreatment culture medium for culturing; 2) pre-culture treatment with a cryoprotectant: transferring the pre-cultured calluses into a composite cryoprotectant for soaking treatment; (3) performing segmented cryopreservation: putting the callus subjected to pre-culture treatment by a cryoprotectant into a sterile cryopreservation tube, adding a composite cryoprotectant, and performing segmented cryopreservation; 4) gradient heating unfreezing: taking out a cryopreservation tube for cryopreservation, carrying out gradient heating unfreezing, and after unfreezing, transferring the callus to a BM2 washing culture medium for washing, and 5) rapid recovery culture: inoculating the washed callus to a recovery culture medium for culture.The method provided by the invention can effectively avoid damage to the callus caused by cryopreservation.

A lyophilized composition capable of encapsulating any nucleic acid with high efficiency and easily is provided. A lyophilized composition of lipid nanoparticles not containing a nucleic acid but containing an ionic lipid, a sterol, a PEG lipid, an acidic buffer component that shows a buffering action at pH 1-6, and a cryoprotectant, wherein a weight ratio of the cryoprotectant and a total lipid is 10:1-1000:1.

The invention relates to an extracellular vesicle-frozen gel scaffold as well as an integrated preparation method and application thereof, and belongs to the technical field of biotechnology and tissue engineering materials. The problems that in the prior art, extracellular vesicles are prone to being damaged by low temperature in the frozen gel preparation process, a traditional cryoprotectant is not compatible with a stent process, and burst release and action time is short in a vesicle physical adsorption method are solved. The method comprises the following steps: mixing extracellular vesicles with a trehalose solution, and standing to obtain a vesicle-trehalose mixed solution; mixing with the support material prepolymer solution, and adding a photoinitiator to form a composite precursor solution; freezing the solution, and performing cross-linking solidification to form a three-dimensional porous gel scaffold; and unfreezing and washing to obtain the stent. The trehalose is introduced as a cryoprotectant, vesicle activity protection and stent structure forming are synchronously achieved in low-temperature forming, and the prepared stent can achieve controllable slow release of vesicles and has good application prospects in the field of tissue repair and regeneration.

A bioink for cryobioprinting is described. The bioink comprises a decellularized extracellular matrix (dECM)-based hydrogel and a saccharide cryoprotectant. A method of making a 3D-printed biomaterial is also described, which includes depositing a bioink comprising a dECM-based hydrogel, cells, and a saccharide cryoprotectant from a 3D printer onto a freezing plate to form a frozen bioink filament; depositing additional bioink from a 3D printer in contact with a previously placed frozen bioink filament to form a 3D-printed biomaterial comprising a plurality of frozen bioink filaments; and removing the 3D-printed biomaterial from the freezing plate. 3D-printed biomaterials made according to the method are also described.

The invention provides a screening method of a low-temperature protective agent, which comprises the following steps: constructing a solution system containing a plurality of single-component low-temperature protective agents, respectively carrying out molecular dynamics simulation to calculate a plurality of microscopic characteristics and target macroscopic characteristics of each solution, and constructing a total microscopic characteristic data set and each target macroscopic characteristic data set; based on clustering and importance analysis and in combination with the corresponding target macroscopic feature data set and machine learning, determining evaluation features for each target macroscopic feature from the total microscopic feature data set, and obtaining a prediction model of each target macroscopic feature at the same time; calculating evaluation characteristics of the low-temperature protective agent to be screened, and respectively inputting the evaluation characteristics into the corresponding target macroscopic characteristic prediction models to obtain corresponding prediction values; predicting the toxicity of the low-temperature protective agent to be screened; and taking the to-be-screened low-temperature protective agent of which all the target macroscopic feature predicted values and toxicity predicted values meet the requirements as a screening result. The method has the characteristics of rapidness, accuracy and low cost.

A lyophilized composition capable of encapsulating any nucleic acid with high efficiency and easily is provided. A lyophilized composition of lipid nanoparticles not containing a nucleic acid but containing an ionic lipid, a sterol, a PEG lipid, an acidic buffer component that shows a buffering action at pH 1-6, and a cryoprotectant, wherein a weight ratio of the cryoprotectant and a total lipid is 10:1-1000:1.

The invention relates to the technical field of animal breeding biology, and discloses a boar semen cryopreservation solution which comprises a boar semen cryopreservation basic diluent, a cryoprotectant and zinc selenide nano-microspheres. The X-ray diffraction pattern of the zinc selenide nano-microsphere is matched with that of a standard card JCPDS37-1463, and the shape of the zinc selenide nano-microsphere is of a multi-level spherical structure with the surface assembled by nano-level particles. The concentration of the zinc selenide nano microspheres is 1 mmol / L. According to the invention, the zinc selenide nano-microsphere with a specific multi-stage spherical structure is applied to the field of cryopreservation of the boar semen for the first time, and the optimal working concentration of the zinc selenide nano-microsphere is determined through experiments. According to the technical scheme, the freezing oxidative stress is effectively relieved from multiple dimensions and multiple target points through the Zn-Se synergistic effect, the comprehensive quality of the frozen and thawed pig sperms is remarkably improved, and an efficient and stable new strategy with a wide application prospect is provided for optimizing the pig sperm freezing preservation technology.

The invention discloses an ultra-low temperature cryopreservation method for tribonema, which belongs to the technical field of microalgae culture and comprises the following steps: culturing algae seeds: inoculating the algae seeds, and carrying out aerobic culture for 7-10 days until a stable phase to obtain an algae solution; algae liquid preparation: diluting the algae liquid with a BBM culture medium, and adding the diluted algae liquid into the sterilized cryopreservation tube; precooling the algae liquid: putting the cryopreservation tube added with the algae liquid into a refrigerator for precooling; adding a composite cryoprotectant: adding the cryoprotectant into the precooled algae liquid cryopreservation tube, and uniformly mixing the cryoprotectant and the precooled algae liquid cryopreservation tube; programmed cooling: putting the cryopreservation tube into a programmed cooling box, and putting the cryopreservation tube into a refrigerator for programmed cooling; and ultralow-temperature cryopreservation: putting the seeds into liquid nitrogen for preserving the seeds. The method is simple, operation is easy, pollution is not prone to occurring, and the breed conservation time is long.

A method for human sperm vitrification is disclosed, which includes providing liquefied human sperm; having a vitrification medium, where the vitrification medium has (a) a sperm buffer, and (b) a cryoprotectant mixture including a permeable cryoprotectant and a non-permeable cryoprotectant; mixing the liquefied sperm with the vitrification medium; providing a sperm straw and loading the straw with 0.25 to 0.5 mL of the obtained mixture; sealing the straw with heat; and placing the straw vertically in a container with liquid nitrogen to vitrify the mixture. A portable kit is also disclosed, which makes it easy to implement the method. The kit includes a vitrification medium, some straws, a container, usage instructions, among others.

This invention relates to the field of biomedical technology and provides a controllable cryogenic treatment mesenchymal stem cell preparation, its preparation method, and its application. The method includes the following steps: cell preparation and cryopreservation equilibration: digestion and collection; cryopreservation equilibration; removal of cryoprotectant and washing: centrifugation to remove cryopreservation solution; PBS washing; cryogenic incubation: resuspending the final cell pellet after multiple washes in pre-cooled PBS; incubating the cell suspension at 4±0.5℃ for 72 hours±10 minutes; after incubation, removing the cell suspension from the 4℃ environment, centrifuging, and resuspending in PBS. This invention yields a ready-to-use, easily stored and transported, and functionally enhanced RT-MSCs preparation. Although the cells in this preparation have lost their proliferative and metabolic activity, they still retain the complete therapeutic functions of MSCs, such as immunomodulation, anti-fibrosis, and angiogenesis promotion.

The present invention relates to the compositions, methods, and associated devices (1) for the cryopreservation of biological specimens that enables to freeze and thaw the entire specimen by straightforwardly protecting its three-dimensional architecture. The invention comprises compositions including reagents to protect the specimen and preserve the physiological conditions. The methods comprise fewer steps excluding the need for other solutions used in conventional methods. The methods also eliminate stressful steps for biological specimens in conventional methods including harvesting, pipetting, centrifuging, and transferring. With this invention, the entire content of the specimen can be frozen, stored long-term in a single cell culture vessel with one of the associated devices (1) described here, and then thawed while still in the device (1). The biological specimens frozen by that method render high post-thawing viability. Moreover, the invention reduces cryoprotectant-related toxic events, human errors, and contamination risk.

The invention discloses a low-temperature preservation method of Octopus variabilis sperms and spermatophore, the Octopus variabilis sperms adopt an ultralow-temperature cryopreservation method, the Octopus variabilis sperms are protected by using a cryoprotectant composed of a 0.6 mol / L glucose solution diluent and 20% glycerol, the diluent contains nutrient substances, and the Octopus variabilis sperms have the characteristics of inactivation of sperms and small damage to sperms. The cryoprotectant has the functions of a permeable antifreeze agent and a non-permeable antifreeze agent, so that the sperms are subjected to dual protection in the freezing process; the octopus variabilis spermatophore is preserved at a low temperature, a 1.0 mol / L glucose solution is used as a spermatophore protection solution, the spermatophore structure can be maintained within one month, and high sperm activity of octopus variabilis sperms can be maintained within a short period (one month). Based on requirements in experiments, two octopus variabilis sperm preservation methods are provided and can be flexibly used, and technical support is provided for preservation of octopus variabilis germplasm resources and overcoming of the problem of asynchronous male and female development.

The application provides a method for detecting the content of dimethyl sulfoxide and ethylene glycol in vitrification freezing liquid, and the content of dimethyl sulfoxide and ethylene glycol in vitrification freezing liquid is rapidly detected by using gas chromatography analysis method.The beneficial effect of the application is that the content of dimethyl sulfoxide and ethylene glycol in vitrification freezing liquid is rapidly detected, and the detection efficiency of vitrification freezing liquid is improved.

The present invention provides a method for freeze-drying Lactiplantibacillus plantarum BS25 (LPBS25) to produce a lyophilized probiotic powder with high cell viability and extended shelf life. The method involves suspending LPBS25 cells in a cryoprotectant solution comprising 2.184% sorbitol, 5.112% trehalose, and 1.036% inulin, followed by a multi-step freezing process at -18°C and -80°C, and vacuum desiccation for six hours. Response Surface Methodology (RSM) was employed to optimize the cryoprotectant combination, achieving a survival rate of 96.37% and viable counts of 2.06×1011 CFU / g. The lyophilized powder maintains viability above 1.0×109 CFU / g during storage at -18°C or 4°C for up to one month and demonstrates suitability for probiotic applications. The present invention further includes methods for assessing cell viability, calculating survival rates, and evaluating shelf life to ensure the efficacy and stability of the probiotic product.

The ultralow-temperature cryopreservation method comprises the following steps: taking dwarf at a breeding period, performing drying in shade and variable-temperature stimulation to enable the dwarf to discharge sperms naturally, filtering, centrifuging, adding a cryoprotectant into high-density sperms, mixing, and placing on ice to obtain pretreated sperms; the cryoprotectant is composed of seawater and dimethyl sulfoxide in a volume ratio of 100: (12-20); and placing the pretreated sperms on a liquid nitrogen surface for pre-cooling, and immersing the sperms in liquid nitrogen for storage. According to the cryopreservation method of the dwarf sperms, the dwarf sperms are unfrozen and recovered under the condition of 60 DEG C, after the frozen sperms are fertilized, the rate of D-shaped larvae reaches 80% or above, the cryopreservation method can be suitable for researching the influence of ultralow-temperature cryopreservation on offspring developed by the frozen sperms, and the offspring obtained by the frozen sperms has the good survival rate, growth rate and the like.

The invention relates to a vitreous material comprising at least one compound of formula (I) or a salt thereof, and at least one polar solvent in a total content ranging from 0.1% to 30% by weight relative to the total weight of the vitreous material. The invention also relates to a method for producing said vitreous material, and to the use thereof as glues, as cryoprotectants and / or thermal protectants for molecular and / or biological elements when temperatures are below the glass transition temperature of said vitreous material, and for moulding water-soluble glass structures with micro-to nano-precision. The invention also relates to a method for producing said vitreous material, and to the use thereof as glues, as cryoprotectants and / or thermal protectants for molecular and / or biological elements when temperatures are below the glass transition temperature of said vitreous material. The invention also relates to a microneedle comprising a vitreous material according to the invention.

The present application relates to the field of biotechnology, and particularly relates to application of L-glutamine in preparation of livestock semen cryoprotectant.The present application provides application of L-glutamine in preparation of livestock semen cryoprotectant, and aims to overcome the shortcomings in the existing semen cryopreservation technology.L-glutamine can effectively reduce the damage of sperm cells in the freezing process, reduce the formation of ice crystals, protect the integrity of the cell membrane, and improve the survival rate, motility and fertilization capacity of the frozen sperm through its excellent antioxidant effect and low toxicity.Meanwhile, L-glutamine has good biocompatibility and strong adaptability, and can be widely applied to the semen cryopreservation of different animal breeds, and provides a safe, efficient and low-toxicity new cryoprotectant selection.