1278 results about "Mannitol" patented technology

The invention discloses construction and application of an engineering bacterium for co-expressing D-mannitol dehydrogenase and D-glucose dehydrogenase, and belongs to the technical field of genetic engineering. The technical problem to be solved by the invention is how to co-express D-mannitol dehydrogenase and D-glucose dehydrogenase through a biosynthetic pathway. In order to solve the technical problem, the invention provides recombinant escherichia coli. The recombinant escherichia coli is characterized by containing a D-mannitol dehydrogenase gene, a D-glucose dehydrogenase gene, a glucokinase gene, an acetyl coenzyme A synthetase gene, a D-lactose transporter gene, a D-glucose PTS permease-free gene and a pyruvate oxidase-free gene. And no regulator gene of galactose operon is contained. The invention establishes a preparation method for co-producing D-mannitol and sodium gluconate, which has guiding significance for the production of D-mannitol and sodium gluconate.

The invention relates to the technical field of freeze-dried preparations, and provides exosome freeze-dried powder as well as a preparation method and application thereof. The exosome freeze-drying preparation is prepared by mixing an exosome solution and a freeze-drying protection solution and then freeze-drying the mixture, the concentration of the exosome solution in the mixed solution is 0.1-10% V / V, and the freeze-drying protection solution comprises 0.4-4% W / V of saccharides and 0.25-4% W / V of alcohols; the saccharide is trehalose, cane sugar or lactose; the alcohol is mannitol, sorbitol or a combination of mannitol and polyethylene glycol; and the solvent is sterilized water for injection or a PBS buffer solution. The exosome freeze-dried powder is stable in activity, small in irritation, good in safety and good in fibroblast proliferation promoting capacity, when the exosome freeze-dried powder is applied to supporting essence or masks in the medical beauty industry, the exosome activity of the exosome freeze-dried powder is still kept stable, and the application prospect is good.

The invention provides aqueous ophthalmic solutions of phentolamine or pharmaceutically acceptable salts thereof, medical kits, and methods for using such ophthalmic solutions to improve visual performance in a patient. Exemplary aqueous ophthalmic solutions include those containing phentolamine mesylate, mannitol, sodium acetate, and water.

The invention provides aqueous ophthalmic solutions of phentolamine or pharmaceutically acceptable salts thereof, medical kits, and methods for using such ophthalmic solutions to improve visual performance in a patient. Exemplary aqueous ophthalmic solutions include those containing phentolamine mesylate, mannitol, sodium acetate, and water.

The invention relates to the field of agricultural microbial preparations, in particular to a complex microbial inoculant sustained-release granule for preventing and treating sweet potato stem nematode disease and a preparation method of the complex microbial inoculant sustained-release granule. Comprising the following components in parts by weight: 15-25 parts of paecilomyces lilacinus viable bacterium powder, 10-20 parts of bacillus thuringiensis viable bacterium powder, 10-20 parts of streptomyces viable bacterium powder, 5-15 parts of bacillus subtilis viable bacterium powder, 15-25 parts of charcoal, 5-15 parts of sodium alginate, 5-15 parts of humic acid, 5-10 parts of chitosan, 3-8 parts of calcium silicate, 1-5 parts of mannitol and 1-5 parts of sodium lignin sulfonate. Four microorganisms with complementary functions, namely paecilomyces lilacinus, bacillus thuringiensis, streptomycete and bacillus subtilis, are adopted, the sweet potato stem nematode disease is synergistically controlled through multiple action mechanisms, and the control effect and the stability are remarkably improved.

The invention provides a melogabalin besylate tablet and a preparation method thereof, the melogabalin besylate tablet comprises a plain tablet and a coating, the plain tablet comprises, by mass, 4.39% of melogabalin besylate, 75-85% of mannitol 150SD, 3-10% of a disintegrating agent, 0.5-3% of a lubricant, 0.1-0.5% of an adsorbent, 1-10% of a filler, 0.5-3% of an antioxidant A and 0.1-3% of an antioxidant B; and the coating comprises the following components in percentage by mass: 30-45% of talcum powder, 2-10% of lauryl sodium sulfate, 30-45% of glycerol monocaprylate / dicaprylate, 2-10% of titanium dioxide, 30-45% of polyvinyl alcohol, 0.5-2% of iron oxide yellow and 0.5-2% of iron oxide red. The melogabalin besylate tablet prepared by the invention uses specific coating composition and specific coating parameters, so that the melogabalin besylate tablet can be stably stored without a drying agent and a composite aluminum bag for medicine packaging, the stability of the tablet is greatly improved, the complexity of equipment required by production is simplified, and the production cost is greatly saved.

The invention relates to a functional microorganism-containing organic-inorganic compound fertilizer and a production method thereof, and relates to the technical field of fertilizers, the functional microorganism-containing organic-inorganic compound fertilizer comprises an inorganic core layer, an organic coating layer and a functional microorganism outer layer, the functional microorganism outer layer comprises the following components in parts by mass: 10-15 parts of compound functional microorganisms, 5-10 parts of synergistic growth-promoting microorganisms, 2-3 parts of trehalose, 1-2 parts of mannitol, 10-15 parts of sodium alginate, 8-10 parts of chitosan, 2-4 parts of a yeast extract and 30-40 parts of a modified activated carbon carrier. The production method comprises the following steps: preparing an inorganic core layer, preheating the inorganic core layer, and spraying and coating an organic coating layer material to obtain a primary product with an organic coating layer; and coating the surface of the organic coating layer of the primary product with a functional microorganism outer layer material, drying at low temperature, and screening to obtain the organic-inorganic compound fertilizer containing the functional microorganisms. The fertilizer has the effect of improving the slow release effect of the fertilizer and the survival rate of microorganisms.

Veterinary pharmaceutical compositions for direct systemic introduction, also known as DSI pharmaceutical compositions. One veterinary pharmaceutical composition for direct systemic introduction includes about 10-17 dry mass % bovine gelatin; about 10-17 dry mass % mannitol; about 0-1 dry mass % of a surfactant; and about 65-80 dry mass % of the active pharmaceutical ingredient which is a proton pump inhibitor. A method of manufacturing a veterinary pharmaceutical composition for direct systemic introduction includes combining one or more pharmacologically inactive compounds to form a first solution; using one or more surfactants to form a second solution; adding an active pharmaceutical ingredient to the second solution to form a first mixture; adding the first solution to the first mixture to form a pre-formulation; freezing the pre-formulation; and lyophilizing the pre-formulation.

The invention provides a preparation method of mannitol carbonic acid sulfate, mannitol and dimethyl carbonate are used as starting raw materials to prepare mannitol carbonic acid sulfate, a target compound is obtained through ester exchange, cyclization and oxidation reaction, the raw materials are low in price and easy to obtain, the reaction operation is simple and convenient, the yield of the obtained product is 81% or above, the purity is 99.6% or above, and the method is suitable for industrial production. The method is suitable for industrial production.

The invention provides aqueous ophthalmic solutions of phentolamine or pharmaceutically acceptable salts thereof, medical kits, and methods for using such ophthalmic solutions to improve visual performance in a patient. Exemplary aqueous ophthalmic solutions include those containing phentolamine mesylate, mannitol, sodium acetate, and water.

The invention discloses collagen polypeptide firming and anti-wrinkle freeze-dried powder, and relates to the technical field of cosmetics. The invention relates to a biological preservative which is prepared from water, butanediol, a bacillus / soybean fermentation product extract, soluble collagen, acetyl hexapeptide-8, panthenol, 1, 2, 4-trimethyl-1, 3-pentanediol and a proper amount of water. The cosmetic is prepared from 1, 2-hexanediol, tetrahydromethylpyrimidine carboxylic acid, hydroxypropyl tetrahydropyrantriol, tremella polysaccharide, saccharide isomeride, a bifidus yeast fermentation product lysate, sodium hyaluronate, DNA sodium, a radix gentianae extract, mannitol, palmitoyl tripeptide-1, palmitoyl tetrapeptide-7, oligopeptide-1, decapeptide-4, oligopeptide-3, hydrolyzed royal jelly protein, folic acid and disodium hydrogen phosphate. The additive is composed of sodium dihydrogen phosphate, citric acid and sodium citrate. The mask can provide rich nutrients for skin, deeply care the skin, moisturize and relieve the skin, tighten the skin, resist wrinkles and make the skin moisturized, glossy, elastic and tight, and is high in use safety and wide in application prospect.

The present invention relates to the screening of functional probiotics and its application, and specifically provides a strain of Lactobacillus casei RH99 that produces high extracellular polysaccharides and relieves vaginitis, and its preservation number is CGMCC No.32108. The Lactobacillus casei can inhibit the growth of Gardnerella, Candida albicans, and Peptostreptococcus and their biofilm generation ability; the extracellular polysaccharides produced by it have a significant promoting effect on the biofilm formation of Lactobacillus casei itself. RH99 is inoculated into the vagina, and its strain, the metabolites (exocellular polysaccharides) produced, and the core monosaccharide (D-mannitol) can all inhibit the colonization of pathogenic bacteria and promote the colonization of lactic acid bacteria; and RH99 can also relieve vaginitis by inhibiting inflammatory factors. It can be seen that RH99 can inhibit the growth of pathogenic bacteria + inhibit biofilm formation + inhibit pathogenic bacteria adhesion + inhibit the level of inflammatory factors through key core metabolites, and realize the all-round inhibition of four-in-one.

The application relates to an organic-inorganic compound fertilizer containing functional microorganisms and a production method thereof, and relates to the technical field of fertilizers. The fertilizer comprises an inorganic core layer, an organic coating layer and a functional microorganism outer layer. The functional microorganism outer layer comprises the following components in mass fractions: 10-15 parts of compound functional microorganisms, 5-10 parts of synergistic growth-promoting microorganisms, 2-3 parts of trehalose, 1-2 parts of mannitol, 10-15 parts of sodium alginate, 8-10 parts of chitosan, 2-4 parts of yeast extract and 30-40 parts of modified active carbon carriers. The production method comprises the following steps: preparing the inorganic core layer, preheating the inorganic core layer, spraying the organic coating layer material to coat the surface, and obtaining a preliminary product with the organic coating layer; coating the functional microorganism outer layer material on the surface of the organic coating layer of the preliminary product, low-temperature drying and screening, and obtaining the organic-inorganic compound fertilizer containing the functional microorganisms. The application has the effects of improving the slow-release effect of the fertilizer and the survival rate of the microorganisms.

The invention belongs to the field of biology, and discloses a CRISPR / Cas12a freeze-dried microsphere, which is characterized in that the CRISPR / Cas12a freeze-dried microsphere is obtained by freeze-drying the following raw materials: enzyme-free water, 10 * HOLMES Buffer for Cas12a, an RNA enzyme inhibitor, CRISPR / Cas12a protein, crRNA, an upstream primer F, a downstream primer R, a fluorescent probe Reporter and a freeze-drying protective additive; the CRISPR / Cas12a protein is an LbaCas12a protein, and the CRISPR / Cas12a protein is a Cas12a protein; the freeze-drying protective agent is prepared from mannitol, trehalose, polyethylene glycol, glucan and BSA (Bovine Serum Albumin). By further optimizing a freeze-drying protective agent, freeze-drying microballing of a CRISPR / Cas12a system is achieved, the freeze-drying microballing can be matched with RPA constant-temperature amplification double microballing, and detection is conducted through a one-step method. Meanwhile, the invention further discloses a three-microsphere detection system, application and a kit.

The invention relates to the technical field of eutectic crystals, in particular to a lutein and mannitol eutectic crystal as well as a preparation method and application thereof, an XRD (X-Ray Diffraction) pattern of the eutectic crystal has characteristic diffraction peaks at least at positions where 2 theta angles are 9.71 degrees, 19.44 degrees, 20.4 degrees, 21.17 degrees, 22.07 degrees, 24.67 degrees, 25.32 degrees, 36.1 degrees, 40.39 degrees and 44.9 degrees, and error tolerance of + / -0.2 degrees exists; a characteristic endothermic peak exists at 163.7 DEG C in a DSC spectrum, and an error tolerance of + / -0.2 DEG C exists; the preparation method comprises the following steps: respectively dissolving mannitol and xanthophyll in water and absolute ethyl alcohol, mixing, carrying out high-temperature and high-pressure reaction, standing, crystallizing, filtering, spray-drying and grinding to obtain the eutectic crystal, and the eutectic crystal can be applied to cosmetics, health-care products, foods and medicines. The eutectic crystal provided by the invention has the advantage of good stability.

The invention provides a freeze-dried mask. The freeze-dried mask is prepared from the following raw materials in parts by weight: 70 to 90 parts of deionized water, 15 to 25 parts of algae extract, 3 to 5 parts of mannitol, 0.5 to 1 part of glycerol, 0.3 to 0.5 part of hydrolyzed collagen, 1 to 3 parts of amino acid mixture, 4 to 6 parts of hydroxypropyl tetrahydropyrantriol, 3 to 5 parts of tetrahydromethylpyrimidine carboxylic acid and 2 to 4 parts of hyaluronic acid. According to the freeze-dried mask disclosed by the invention, natural anti-wrinkle and moisturizing components such as the hydrolyzed collagen, the algae extract, the amino acid mixture and the hyaluronic acid are locked on the mask cloth in a freeze-drying manner, so that on one hand, the freeze-dried mask can better supplement moisture and nutrition for human skin when being used; on the other hand, the freeze-dried facial mask prepared from the natural beautifying and skin-care components is free of any irritating component during use, and does not cause damage to the human skin.

The invention relates to the technical field of medicines, in particular to a neutral calcium supplement preparation. The neutral calcium supplement preparation is prepared from the following raw materials in parts by weight: 20-30 parts of a calcium source, 3-6 parts of multivitamins, 3-5 parts of corn starch, 2-4 parts of magnesium stearate, 4-7 parts of mannitol and 2-5 parts of a flavoring agent, the neutral calcium supplement preparation is prepared by taking a calcium source, multivitamins, corn starch, magnesium stearate, mannitol, a flavoring agent and the like as raw materials. The neutral calcium supplement preparation prepared by the invention not only has better solubility, can relieve the burden of the stomach, but also has excellent easy-to-absorb performance, is convenient to absorb by a human body and has no toxic or side effect, and the quality of the neutral calcium supplement preparation is effectively ensured.

The invention belongs to the technical field of stem cell biological preparations, and particularly relates to an anti-aging compound preparation based on stem cells and application of the anti-aging compound preparation in delaying skin aging. The composition is composed of an inner water phase, a middle oil phase and an outer water phase, wherein the inner water phase is prepared from the following components: stem cell exosomes, hyaluronic acid, nicotinamide ribose, trehalose, mannitol, glycine and a citric acid buffer solution; the medium oil phase is prepared from the following components: medium chain triglyceride, shea butter, vitamin E succinate, PEG (Polyethylene Glycol) modified ceramide NP and cholesterol succinic acid monoester; the external water phase is prepared from the following components: polydatin, cellulose nanocrystals and an emulsifying agent. On the basis of a W / O / W emulsion system, a multi-layer anti-aging mechanism is constructed by utilizing a three-dimensional delivery framework of an inner water phase, an oil phase and an outer water phase and space-time synergy of active ingredients, the exosome permeation efficiency is improved through microneedle introduction, and the anti-oxidation and barrier repair effects are achieved by using multiple ingredients.

The invention provides a battery and an electric device. A positive plate comprises a positive active material, and the positive active material is doped and / or coated with a lanthanum element; the surface of the diaphragm is coated with a ceramic coating; the electrolyte comprises a first solvent, a second solvent, a first additive and a second additive; the first solvent comprises a cyclic carbonate compound, the second solvent comprises a chain fluorosulfonamide compound, the first additive comprises mannitol carbonate sulfate, and the second additive comprises succinonitrile; the lanthanum element is doped / coated on the positive active material, so that the release of lattice oxygen at high voltage or high temperature can be effectively inhibited, and the exothermic reaction activity at the thermal runaway initial temperature is reduced; the first additive and the second additive in the electrolyte respectively form a compact CEI film and an SEI film with high thermal stability on the surfaces of the positive electrode and the negative electrode; the diaphragm ceramic coating provides a physical barrier, the thermal shrinkage temperature of the diaphragm is increased, and internal short circuit is prevented.

The invention discloses a low-temperature vacuum microwave freeze-dried sinomenine hydrochloride preparation and a preparation method thereof in the technical field of pharmaceutical preparations, and solves the problems of long drying time, high energy consumption and poor product stability in the traditional freeze-drying technology. A vacuum microwave collaborative freeze-drying technology is adopted, microwave internal heating is achieved through a 2.45 GHz magnetron microwave generator and a rectangular waveguide tube under the conditions that the vacuum degree is 20-30 Pa and the plate layer temperature ranges from-40 DEG C to-35 DEG C, the microwave power density in the primary drying stage ranges from 0.3 W / g to 0.5 W / g, the microwave power density in the secondary drying stage ranges from 0.1 W / g to 0.3 W / g, and collaborative control over the microwave power, the vacuum degree, the temperature and the time is achieved in cooperation with a quaternary coupling precise control system. The preparation contains 50 mg of sinomenine hydrochloride, 100 mg of mannitol and 10 mg of sodium carboxymethyl cellulose grafted modified polyvinylpyrrolidone, the residual moisture is controlled to be 0.4-0.6%, the activity retention rate is 94-96%, and the drying time is shortened to 12-16 hours.

The invention provides melogabalin besylate tablets and a preparation method thereof. The melogabalin besylate tablets comprise melogabalin besylate, a filler, a disintegrating agent, an acid stabilizer, an antioxidant, a lubricant, a flow aid and a gastric-soluble film coating agent. The antioxidant comprises gallic acid, the acid stabilizer comprises maleic acid, and the filler comprises directly pressed starch, isomaltitol, mannitol and microcrystalline cellulose. By screening the antioxidant and the acid stabilizer, the stability of the tablet in the storage period is improved.

The invention relates to the field of pharmaceutical preparations, in particular to a diclofenac sodium sustained release tablet and a preparation method thereof. The diclofenac sodium sustained release tablet is prepared from the following components: diclofenac sodium, hydroxypropyl methyl cellulose, sodium carboxymethyl cellulose, mannitol, organic acid salt, a flow aid and a lubricant. The diclofenac sodium sustained-release tablet provided by the invention can be stably released, has an excellent sustained-release effect, and is simple in preparation process, low in cost and suitable for industrial mass production.

The invention discloses a recombinant III-type human collagen composition. The recombinant III-type human collagen composition comprises recombinant III-type human collagen, a first buffer solution and a stabilizer, the stabilizer is selected from at least one of glycerol, butanediol, pentanediol, mannitol and trehalose. According to the recombinant III type human collagen composition, a chemical cross-linking agent or an enzymatic promoter is not added for cross-linking, and the used reagents are free of cytotoxicity, sensitization and other safety risks. Therefore, the recombinant III-type human collagen composition has no crosslinking agent residue, can form a three-dimensional network structure, can avoid the problem that the recombinant III-type human collagen and the polysaccharide are compounded to generate precipitates, also has good biocompatibility, degradability and high stability, especially has relatively high thermal stability, and can be used for preparing the recombinant III-type human collagen composition. The wound healing and repairing can be promoted, the healthy skin ecology can be reconstructed, and a new choice is provided for a biomedical material.

The invention discloses a liver function biochemical composite quality control product as well as a preparation method and application thereof. The quality control product comprises 12 analyte raw materials and the following non-analyte raw materials: 5 to 12 g / L of sodium chloride, 0.5 to 2 g / L of casein sodium salt, 5 to 20 g / L of mannitol, 0.05 to 0.2 g / L of Tween 20, 1 to 20 mg / L of bright aprotinin, 10 to 60 g / L of trehalose and 0.05 to 0.2 g / L of PC300. And matrix liquid: 20-80% of quality control substance matrix liquid and 20-80% of buffer solution. The liver function biochemical composite quality control product disclosed by the invention has high compositing property, contains 11 important liver function biochemical indexes, is excellent in component uniformity and stability, ensures the precision of each index, and meets the requirements of daily quality control.

The invention relates to the technical field of purification of fine chemical intermediates, in particular to a purification method of a mannitol carbonate sulfate preparation process intermediate, which comprises the following steps: S1, synthesizing diacetone-D-mannitol; s2, preparing an organic solution containing solids; s3, performing deprotection reaction to prepare a solid crude product; s4, removing unreacted solids; s5, extracting and separating by-product solids; and S6, purifying the target product solid and recovering mannitol. Through system separation and multi-component recovery, preparation of a high-purity product and cyclic utilization of unreacted substances, byproducts and raw materials are realized, the yield is remarkably improved, the cost is reduced, and the quality of a final product is guaranteed.

The present invention relates to a high-temperature starch-type wetting agent and a preparation method and application thereof, wherein the wetting agent comprises an active ingredient and water, wherein the mass ratio of the active ingredient to the water is (0.05-0.15): 1, and the active ingredient comprises the following raw materials in parts by weight: 45-65 parts of a starch-based film-forming agent, 5-10 parts of a plasticizer, 15-20 parts of a lubricant, 1-2.5 parts of an emulsifier, 4-6 parts of a coupling agent, 0.5-1.5 parts of a preservative, and 0.02-0.05 parts of a defoaming agent; wherein the plasticizer is cardanol acetate, tributyl citrate, and mannitol in a mass ratio of 1:1:(1.5-2); and the starch-based film-forming agent is at least one of acetylated distarch adipate, oxidized starch, and hydroxypropyl starch. The glass fiber yarn products produced with the wetting agent have significantly reduced scattered silk defects on the cloth surface, and the amount of powder falling during the weaving process is also greatly reduced, which meets the high standard requirements for the production of printed circuit boards.

The invention discloses a separation and instantaneous conversion method of green Chinese onion leaf protoplast, and belongs to the technical field of protoplast preparation, the method comprises the following steps: 1) culturing green Chinese onion leaves; 2) separation and purification of green Chinese onion leaf protoplast; 3) determining the yield and vitality of the green Chinese onion leaf protoplast; 4) determining an optimal enzymolysis condition; and (5) PEG-mediated protoplast transformation: determining the optimal concentrations of cellulase, macerozyme and mannitol in the enzymolysis process, and determining the optimal enzymolysis time, so that the separated green Chinese onion leaf protoplast is higher in yield and activity, and the separated green Chinese onion leaf protoplast can not only be used for instantaneous transformation, but also can be used for enzymolysis of the green Chinese onion leaf protoplast. And a technical support is provided for research on molecular biology of the green Chinese onions.

The invention relates to the technical field of crop cultivation, and particularly discloses a method for increasing the anthocyanin content of triticale grains, which comprises the following steps: respectively spraying 80-100 times of leaf fertilizer diluent once at the elongation stage and filling stage of triticale growth, and spraying 6-10kg / mu each time; the formula of the foliar fertilizer is as follows: 100 mg to 150 mg of mannose, 100 mg to 150 mg of fructose, 130 mg to 160 mg of mannitol, 160 mg to 200 mg of betaine, 180 mg to 200 mg of alginic acid, 180 mg to 200 mg of fucoidin, 300 mg to 350 mg of amino acid and 1000 g of water. According to the method, the content of anthocyanin in triticale grains can be remarkably increased by spraying 80-100 times of foliar fertilizer diluent prepared by the method in the elongation stage and the pustulation stage of the triticale.

The invention relates to the technical field of pharmaceutical preparations, and particularly discloses a bisoprolol and amlodipine compound tablet and a preparation method thereof.The bisoprolol fumarate and amlodipine besylate compound tablet is prepared by mixing bisoprolol fumarate with a disintegrating agent, a flow aid and a filling agent, mixing amlodipine besylate with the disintegrating agent, the filling agent and a lubricating agent and then conducting dry granulation; wherein bisoprolol fumarate is controlled to have a large particle size so as to improve the mixing uniformity, amlodipine besylate is controlled to have a small particle size so as to carry out dry granulation, amlodipine can be wrapped in an auxiliary material, and the contact between amlodipine and bisoprolol in the mixing process is reduced. More importantly, by adopting the filler mannitol with lower hygroscopicity, the moisture of the tablet can be reduced in the production and storage processes, the generation of impurities can be reduced, meanwhile, the particle size of the filler is controlled, the physical contact between bisoprolol and amlodipine can be reduced, the impurity product formed by the mutual reaction between bisoprolol and amlodipine can be reduced, and the stability of the medicine is remarkably improved.

The invention provides an anti-freezing denaturation agent for golden and tiger hybrid spotted fish meat protein and a preparation method of the anti-freezing denaturation agent. The invention relates to an anti-freezing denaturation agent for protein of Jinhu hybrid spotted fish. The anti-freezing denaturation agent is prepared from the following raw materials in parts by weight: 5 to 8 parts of trehalose-mannitol, 0.5 to 1 part of nano chitosan, 0.2 to 0.5 part of rosmarinic acid and 0.1 to 0.2 part of polysorbate-80. The trehalose-mannitol is prepared from trehalose and mannitol. Aiming at the characteristics of Jinhu hybrid spotted fish meat, trehalose-mannitol, nano chitosan, rosmarinic acid and polysorbate-80 are taken as raw materials and compounded to obtain a new formula of the protein anti-freezing denaturation agent for the Jinhu hybrid spotted fish meat, and the anti-freezing denaturation agent prepared by adopting the formula and the process disclosed by the invention can be used for inhibiting protein degradation and improving the anti-freezing denaturation effect of the Jinhu hybrid spotted fish meat. The decrease range of MP protein concentration, MP total sulfydryl content and MP Ca < 2 + >-ATPase activity of the antifreeze agent is obviously reduced, and the effect of the antifreeze agent is superior to that of a commercially available antifreeze agent. Moreover, the anti-freezing denaturation agent for the protein of the Jinhu hybrid spotted fish does not contain phosphorus, and has the advantages of safety and high efficiency.