The invention discloses a [3,5-disubstituted phenyl-1-(1,2,4-triazole)]benzenesulfonic acid derivative, a preparation method and applications thereof, wherein a benzoic acid raw material is substituted, an acylation reaction and a condensation cyclization reaction with substituted benzamide are performed, and a reaction is performed with a p-hydrazinobenzenesulfonic acid derivative to obtain a target product [3,5-disubstituted phenyl-1-(1,2,4-triazole)]benzenesulfonic acid derivative. According to the present invention, the compound can effectively antagonize nerve cell oxidative stress damagecaused by hydrogen peroxide, sodium nitroprusside, iron ions and copper ions, can repair mitochondrial function by scavenging free radicals and restoring mitochondrial membrane potential, and has significant protection effect on neuronal cells; and the compound has neuroprotective effects of free radical scavenging and targeting mitochondria protection, and can be used for preparing drugs for prevention and treatment of neurodegenerative diseases such as stroke, brain injury, spinal cord injury, Parkinson's disease, Alzheimer's disease, amyotrophic lateral sclerosis, and the like.