630 results about "Biomedical technology" patented technology

The invention is a manufacturing method for quanta point microsphere used as biomedicine fluorescence probe. It belongs to chemical engineering, material and biomedicine field. The invention uses spray and drying method to embeds the quanta point into high molecular material, forms quanta microsphere with particle distribution less than 10 micrometers, the quanta point nanometer particle with different quantity and different fluorescence character is dispersed in the high molecular liquid, then carries on spray and drying, it changes the fluorescence character of the microsphere through changing type of quanta point material, quantity of the quanta point and the color and intensity of the quanta point, controls the particle size and figure of the quanta microsphere through adjusting the condition of spray and drying.

The invention belongs to the technical field of molecular biology and biomedicine and in particular relates to application of a gRNA (guide Ribonucleic Acid) sequence based on a CRISPR / Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats / CRISPR associated protein 9) system on knockout of a human Lin28A gene and application to tumor treatment. According to a design principle of CRISPR / Cas9, 6 guide RNAs (gRNA) are designed and a sequence table is shown as SEQ ID NO.1 to SEQ ID NO.6; the guide RNAs are constructed on a PX458 carrier and two efficient target gRNAs are obtained through activity detection and screening. The CRISPR / Cas9 system guided by the two gRNAs is utilized in human hepatocellular carcinoma cell lines (HepG2) and human melanoma cell lines (A375), and the human Lin28A gene can be effectively knocked out; the system is easy to operate and the human Lin28A gene knockout efficiency is high, so that the system is applicable to various tumor cell models. The gRNA provided by the invention is hopefully applied to a new drug for treating tumors.

The invention belongs to a biomedical technology field. The invention provides an anti-human ErbB2 antibody and ansamitocin conjugate, comprising: substituting lysine at 30 or / and 65 position in a heavy chain variable region of Trastuzumab with a hydrophilic amino acid, and then coupling with ansamitocin (DM1). On basis of maintaining coupling Pharmaceutical activity, a proportion of ansamitocin in a coupling medicament is reduced, thereby reducing toxic and side effect of the coupling medicament. The invention further provides applications of the anti-human ErbB2 antibody and ansamitocin conjugate for preparing antitumor drugs.

The invention discloses calcium supplement health-care tea oil, relating to the field of biomedical technology. The calcium supplement health-care tea oil is prepared from tea oil, dried small shrimp and calcium carbonate. Specially, the preparation method comprises the steps of: auxiliary material pretreatment, auxiliary material retreatment, auxiliary material mixing, mixed auxiliary material treatment, extraction, purification and filtering. The calcium supplement health-care tea oil has the characteristics of complete nutrition, capability of supplementing calcium, and the like, not only can be suitable for the calcium-deficient crowds to eat, but also can be suitable for children and the old to eat.

The invention belongs to the technical field of nano materials and biomedicine, in particular to multifunctional core-shell structure fluorescent coding magnetic microspheres and a preparation method thereof. On the basis of ferroferric oxide nanoparticles synthesized by a hydrothermal process, pre-prepared coupled product of fluoresceins and amino propyl trimethoxysilane and ethyl orthosilicate are subjected to cohydrolysis in ammonia water to form a multifunctional fluorescent magnetic nano composite material with fluorescent and magnetic properties and high biological stability and biological adaptability. By regulating the mixing ratio of fluoresceins, namely fluorescein isothiocyanate (FITC) and rhodamine B isothiocyanate (RBITC), various fluorescent coding magnetic microspheres can be prepared, and the particle size of obtained material can be regulated according to the different ratios of the added ethyl orthosilicate to the ferroferric oxide. In addition, the obtained multifunctional composite nano material is subjected to amino silanization modification, so the biological application range of the novel fluorescent coding magnetic microspheres is further expanded and the novel fluorescent coding magnetic microspheres have bright application prospect in fields of biomedicine technology, medicine development, suspension chip and the like.

The invention relates to a cell model obtained after targeted knockout of a rabbit BMP2 gene based on CRISPR / Cas9 and application thereof, belonging to the technical field of molecular biology and biomedicine. According to the invention corresponding oligos are synthesized for three targeting sites of the rabbit BMP2 gene on the design principles of CRISPR / Cas9 and are constructed on px458 vectors; and a CRISPR / Cas9 system is constructed directed at the three targeting sites in rabbit mesenchymal stem cells, and the CRISPR / Cas9 system can effectively knock out the rabbit BMP2 gene, is easy to operate and has high rabbit BMP2 gene knockout efficiency. The cell model disclosed in the invention can greatly promote research related to the functions and signaling pathways of the BMP2 gene.

The invention relates to a biomedicine missing data compensation method based on an EM cluster-BP neural network and belongs to the technical field of biomedicine. According to the method, an iterative algorithm EM algorithm which is important in processing incomplete data problems is adopted, different missing data are partitioned in different clusters according to an EM cluster and primary compensation is completed; and models are established for complete data in each cluster through a BP neural network method and precise compensation of the missing data in each cluster is finished. According to the biomedicine missing data compensation method, adaptability of a compensation algorithm to any missing mechanism is enhanced to a certain degree, accuracy of compensation is improved and the method is suitable for the biomedicine missing data compensation field.

The invention belongs to the technical field of the molecular biology and the biomedicine, and particularly relates to application of a gRNA sequence based on a CRISPR / Cas9 system to cause tumor cell apoptosis. According to the design principle of CRISPR / Cas9, two target points are designed on a human genome, and corresponding oligos is synthesized and established on a px458 carrier. The CRISPR / Cas9 system guided by the gRNA is established in a human hepatoma cell line (HepG2) according to the design of the two target points, a human TCAB1 gene can be effectively knocked out, the system is easy to operate, and the knockout efficiency of the human TCAB1 gene is high. The CRISPR / Cas9 system guided by the gRNA can be expected to be applied in novel tumor treating medicine.

The invention discloses a macromolecular microcarrier and a preparation method thereof, belonging to the technical field of biomedicine. The preparation method comprises the following steps of: splitting a silk solution and a drug solution into micrometer grade liquid drops with core-shell structures under the action of a high-voltage electric field by adopting a coaxial high-voltage electrostatic technology and a freeze drying method; concreting through liquid nitrogen, and then preparing the microcarrier which is difficult to dissolve in water through freeze drying. The microcarrier is in the shape of a microsphere with the core-shell structure, and the diameter of the microsphere is 100-500 micrometers; the shell of the microcarrier comprises the components of silk fibroin; the random-coil conformation of silk fibroin molecules is 80-90 percent; the shell is in a porous structure, and an aperture is 5-20 micrometers; and a core of the microcarrier comprises the components of water-soluble drugs. The silk microcarrier has extensive application prospect in the fields of cell culture, drug release, and the like.

The invention belongs to the technical field of molecular biology and biomedicine, and relates to a method for increasing the efficiency of CRIPSR / Cas9 target knock-out genes NHEJ. According to the method for increasing the efficiency of CRIPSR / Cas9 target knock-out gene NHEJ, small molecule interference RNA that efficiently interferes with expression of PTEN genes is screened out; by using CRISPR / Cas9 system to target knockout genes, the small molecule RNA is cotransfected into the target gene, thus the NHEJ efficiency of target genes is effectively improved and the NHEJ of target gene in cell multiple targets is significantly improved. The method has the advantages of low cost, simple operation and high efficiency, and has a good promoting effect on the efficiency and application of CRISPR / Cas9 technology.

The invention provides a method, primer and plasmid for constructing an EPO gene knockout zebrafish animal model and a preparation method of the primer and the plasmid, and belongs to the field of biomedicine. The method includes the steps of 1) establishment of an EPO gene knockout zebrafish CRISPR oligomer sequence plasmid; and 2) establishment and culture of the EPO gene knockout zebrafish animal model on the basis of CRISPR gene knockout technology; wherein the step 1) comprises 1-1) design and synthesis of the primer aiming at an EPO exon 2 region target sequence and 1-2) synthesis of the Escherichia coli plasmid containing the EPO target sequence. The method for constructing the EPO gene knockout zebrafish animal model can be used to construct the EPO gene knockout zebrafish animal model, and facilitates further understanding and research of EPO.

The present invention provides a neonatal pain identification method based on facial expression analysis, which can identify whether a newborn is painful or not. The method comprises: obtaining a video sequence containing facial information of a human face; extracting facial dynamic geometric features and facial dynamic texture features that represent dynamic changes in pain facial expressions from the obtained video sequence; carrying out feature fusion on the extracted facial dynamic geometric features and the facial dynamic texture feature, and carrying out dimension reduction on a facial feature vector obtained after the fusion; and according to the facial feature vector obtained after the dimension reduction, training a classifier. The present invention relates to the technical field of pattern identification and biomedicine.

The invention discloses a drug-loaded microsphere and the preparation method, belonging to the technical field of biomedicine, which comprises following steps: adopting water / oil / water multiple emulsion technology and self-assembly technology of protein to fully mix the water soluble medicine and regenerated silk fibroin solution; adding the mixture in oil phase with emulsifier with certain amount during stirring for emulsification; adding organic solvent and stirring, thereby the silk fibroin is denaturalized and Beta structured to form milky crystalline silk fibroin fine particle, and the medicine is embedded; removing supernatant liquid through centrifugal effect; adding organic solvent; further crystallizing the silk fibroin and embedding the medicine; removing solvent and residual oil phase through centrifugal effect and collecting the microsphere. The average particle size of the silk fibroin drug-loaded microsphere is between 5.84 to 86.27Mum, the embedding rate is 42 to 99%, the drug loading dosage is 2.5 to 8.5%. The drug-loaded microsphere has the advantages of applicability to requirements for different purposes and wide application prospect.

The invention relates to a method for judging 2 type diabetes mellitus risk state and belongs to the technical field of biomedicine. The method includes that EM clusters and C4.5 classification are utilized to extract determinant attributes affecting attack of the 2 type diabetes mellitus, the crowd is divided into different cluster groups according to own characteristics, according to determinant attributes extracted results and a Logistic regression method, personal or crowd risk state is judged by combining metabolic syndrome and high risk group criterions. The determinant attributes affecting the 2 type diabetes mellitus are obtained, simultaneously, different risk state judgment models are built based on different characteristics of the crowd, the universality and practicability of the models are improved, the requirements of real-time processing and mobile computation are met, the effective degree of the determinant attributes is estimated through a quantitative analysis method, the complexity is low, the accuracy is high, and the divided risk state is detailed.

A blood purification sorbent for antibody removal belongs to the technical field of biomedicine, which consists of the two parts of solid-phase carrier material and a petunidin fixed on the carrier by chemical coupling. The molecular structure of the petunidin is shown as above, wherein, one atom among A, B and C is N, the others are C; n is 0-2. The blood purification sorbent can adsorb the antibody component in plasma and autoantibodies such as rheumatoid factor, antinuclear antibody, etc. in heavy load, has limited nonspecific adsorption to other plasma components such as seralbumin, etc., and also has low preparation cost and stable physicochemical property. The material can be used as adsorption filler of a blood purification device for removing the autoantibody and immune complex in the plasma.

The invention discloses an aqueous solution of silk fibroin and a preparation method for the aqueous solution, and belongs to the technical field of biomedicine. According to the technical scheme, the method comprises the following steps of: adding strongly polar polyol or soluble salt for hindering gelatinization of a silk fibroin solution into the silk fibroin solution, performing high-temperature steam sterilization, and thus obtaining the aqueous solution of the silk fibroin, wherein the aqueous solution of the silk fibroin can be stored for over 3 months at room temperature without gelatinization or deterioration and has stability. The aqueous solution of the silk fibroin is suitable to be prepared into various silk fibroin films, hydrogel, porous materials, microspheres and the like used for cosmetics, skin-care products, sun lotion, tissue engineering materials, medicament carriers, artificial skin and the like. Because the stable aqueous solution of the silk fibroin can be stored for long time, the preparation-and-use workshop type production mode of a silk fibroin biomaterial can be changed into a large-scale industrialized production mode.

The invention relates to a reducible and degradable nano medicine-carrying micelle and a preparation method thereof, belonging to the fields of biomedical technology and nano medical technology. The reducible and degradable nano medicine-carrying micelle is characterized by having the following structural general formula: MPEG-CA-PCL, wherein the CA is poly-cystamine containing disulfide bonds, the MPEG is methoxy polyethylene glycol the molecular weight of which is 475-5000DA, and the PCL is polycaprolactone. The preparation method of the reducible and degradable nano medicine-carrying micelle comprises the following steps: (1) carrying out Michael addition to N,N'-bis (acrylic) cystamine and ethanolamine to obtain hydroxy poly-cystamine containing disulfide bonds, and then, carrying out ring opening reaction on caprolactone by utilizing a hydroxy group to obtain a CA-PCL polymer; and (2) mixing the CA-PCL polymer obtained in the step (1) and carboxyl-terminated MPEG, and adding paclitaxel (PTX) to prepare the nano medicine-carrying micelle through a dialysis method. The method is efficient and simple.

The invention belongs to the technical field of biomedicine and discloses a nanometer magnetic immunomicrosphere for enriching circulating cancer cells and a use method thereof. A nanometer carbon coated pure ferromagnetic microsphere (Fe@C) is taken as an inner core and is connected with a cancer cell antibody, so as to prepare the nanometer magnetic immunomicrosphere for enriching the circulating cancer cells. By utilizing the magnetic immunomicrosphere to enrich the circulating cancer cells, the sensitivity of the detection for the circulating cancer cells is efficiently increased. The immunomicrosphere has the characteristics of strong magnetic reactivity, easiness in operation, and the like, and is applied to the quick enriching of the circulating cancer cells.

A colloidal gold immunity chromatograph to be used for the inspection of aquatic product, agricultural product and livestock product belongs to the technical field of biological medicine. The presentinvention with high sensitiivty and high specificity and be widely used in the departments of inspection and epidemic prevention to overcome the problems such as short of inspecting means and limitation by the conditions existed in the product inspection for the quatic, and livestock agricultural prdoucts.

Belonging to the technical field of biomedicine, the invention in particular relates to a natural antibacterial peptide QHA and application thereof. The natural antibacterial peptide QHA is composed of 30 amino acid residues, is straight chain polypeptide, and has a molecular weight of 3628.5Da and isoelectric point of 12.61. The antibacterial peptide QHA provided by the invention has the beneficial characteristics of small molecular weight, simple chemical synthesis method, efficient, broad-spectrum and efficient antibacterial action, and good stability, etc., thus having broad application prospects. In addition, the invention also relates to a coding gene of antibacterial peptide QHA and application thereof.

The invention relates to a method for amplifying cells, in particular to an in vitro amplification adjustable T cell method, belonging to the technical field of biomedical science. The invention uses Rapamycin combined with TGF-beta to induce human T cell in vitro to become adjustable T cell having immune suppression function, having the following advantages in : 1. enough quantity; and 2. capability of resisting differentiation towards Th 17 cell. The invention overcomes plural defects of natural adjustable T cell, and has larger curing advantages in curing inflammation diseases, autoimmune diseases and prevention of immunity rejection of organ implantation.

The invention belongs to the technical field of biomedicine and discloses an immunochromatographic device and a detection method using the same. The immunochromatographic device is composed of an immunochromatographic test strip and a reaction tank. In the immunochromatographic device, quick, ultrasensitive and quantitative detection of a target substance can be achieved by virtue of time distinguishing detection equipment by taking a rare-earth fluorescence nanoparticle as a marker. Compared with the traditional immunochromatographic test strip, the immunochromatographic device has high sensitivity. The device can be widely applied to quick, qualitative and quantitative detection items in the fields of biology, medicine, food safety and the like.

The present invention involves multifunctional nanoparticle dispersions and methods for making them using sol-gel chemistry, doping, and sonication. These methods avoid the high thermal budget processes of the reference art. The dispersions can accommodate greater concentrations of nanoparticles, dopants, and ions than has previously been possible since these components can be added during synthesis. The unique optical, magnetic, luminescent, metallic, insulating, semi-conducting, and / or conducting properties of these particles can be utilized to enhance photovoltaic cells, portable electronic devices, and biomedical techniques among other applications.

This invention discloses cryo-heating phototreatment devices and methods for cooling and heating objects in a very rapid timescale, from femtosecond to subsecond. The cryo-heating phototreatment device is a multiplex energy source comprising of an electromagnetic source and at least one of the members of the group of radiation sources: sonic, magnetic, electric, electroluminescent, up-converted luminescence, pressure and thermal. The electromagnetic source in the cryo-heating phototreatment device induces cooling of objects by up-converting energy luminescence and heating by absorption or interaction of electromagnetic radiation with objects. The invention also proposes a method of an enhancement of the up-converted energy cooling and heating processes by surface plasmon resonance of conducting nanostructures. The proposed devices and methods of very rapid cooling and heating can be applied in biomedical technologies and health care. The invention also includes applications of the disclosed herein method to up-conversion energy cooling of electronic components and photon detector devices.

The invention relates to an elderly cognitive function classification method based on a random forest and belongs to the technical field of biomedicine. The method disclosed by the invention comprises the following steps: dividing the elderly cognitive function into three types by adopting MMSE scale scores and education levels; extracting a key cognitive domain influencing elderly cognitive function category classification by utilizing a cognitive function score relative ratio calculation method and a Pearson linearly dependent coefficient calculation method; establishing a random forest regression model, calculating attribute significance scores of non-scale attributes, and extracting external related attributes influencing the elderly cognitive function category classification; finally, equalizing the sample set based on the extracted key cognitive domain and external related attributes by adopting an SMOTE up-sampling method, and establishing an elderly cognitive function classification model by utilizing the random forest method. Compared with a scale classification method, the method provided by the invention has the advantages that the adopted attributes are few and easy to collect, and the method has high convenience; compared with other machine learning algorithms, subdivision of the elderly cognitive function types is realized, and research of a method for performing targeted intervention on the elderly cognitive functions is facilitated.

The invention belongs to the technical field of biomedicines, and in particular relates to application of seaweed polysaccharides. The seaweed polysaccharides can be taken as preparations for preparing anti-viral or immune medicines. Cell models and in-vivo animal experiments show that the seaweed polysaccharides can be used for significantly enhancing the animal immunity, and can also be used for promoting the generation of cell factors, the typing of T lymphocytes and the proliferation of mouse spleen cells so as to ensure that cellular immune reaction can be activated. The functional seaweed polysaccharides disclosed by the invention are natural polysaccharides which are extracted from seaweeds such as large seaweeds and kelps, gulfweeds, grateloupia sparsa, eucheuma muricatum, ulva pertusa kjellm, enteromorpha, gracilaria, ascophyllum nodosum and scytosiphon lomentaria, and can also be low-molecular-weight seaweed polysaccharides or seaweed oligosaccharides which are prepared by degrading the seaweed polysaccharides by virtue of different preparation methods. According to the application disclosed by the invention, a polysaccharide extract of single seaweed or a polysaccharide extract mixture of a variety of seaweeds can be taken as a novel anti-viral and immune enhancing agent to be applied to feeds of livestock, poultry, fish, shrimps and shellfish, and has wide application prospects.

The invention belongs to the technical field of biomedicine, and particularly relates to a transformed body antimicrobial peptide HC-15 of antimicrobial peptide Hc-CATH (cathelicidins) of hydrophis cyanocinctus and a preparation method and application of the transformed body. The transformed body antimicrobial peptide is the transformed body HC-15 of antimicrobial peptide Hc-CATH (cathelicidins) of hydrophis cyanocinctus, is linear chain polypeptide and comprises 15 amino acid residues, the tail end of C- is amidated, the molecular weight is 1974.46Da, and the isoelectric point is 12.61. The transformed body antimicrobial peptide HC-15 has high-potency antimicrobial activity; furthermore, the transformed body antimicrobial peptide HC-15 has beneficial characteristics of small molecular weight, simple structure, low hemolytic activity and simple preparation method.

The invention provides an artificial dura mater spinalis, and a preparation method and a use method thereof. The artificial dura mater spinalis has a double-layer structure and concretely comprises an internal layer and an external layer, the internal layer is an oriented polylactic acid / glycollic acid copolymer nanofiber membrane, the external layer is a non-oriented polylactic acid / glycollic acid copolymer-chitosan nanofiber membrane, and the internal layer and the external layer are connected. The invention belongs to the technical field of the biomedicine. The artificial dura mater spinalis can realize the dura mater spinalis regeneration and prevent epidural scarring and adhesion, and can substantially reduce the postoperative cerebrospinal leakage incidence rate.