1998 results about "Biomedical technology" patented technology

The invention relates to the technical field of biomedicine, and discloses a female private rehabilitation training system integrating a breathing method and neural motor control, and the system comprises a data collection module which is used for collecting physiological data of a user; the personalized training scheme generation module is used for generating a personalized nerve-muscle-fascia training scheme; the nerve-muscle synchronous resonance training module is used for constructing a time sequence matching model and generating an adaptive training dynamic control instruction; the space-time adaptability training module is used for adjusting training duration, intensity and stimulation frequency; the multi-dimensional nerve-muscle feedback fusion module is used for constructing a feedback response model; and the fascia synchronous activation and relaxation module is used for coordinating the tension relationship between the fascia and the muscle system. According to the method, feature matching is carried out on an electric activation heat map matrix through the convolutional neural network, analysis of a myoelectricity response state transition probability is carried out in combination with a hidden Markov model, and stimulation parameters and respiratory rhythm are optimized in real time according to a training process.

The invention relates to the technical field of biomedicine, in particular to a hepatic cell differentiation degree evaluation method based on multi-omics data, which comprises the steps of sample collection and preprocessing, transcriptomics, proteomics and metabonomics data analysis, multi-omics data integration and modeling and result output. By integrating multi-level biological information, a multi-dimensional scoring model is constructed, the liver cell differentiation state is quantitatively evaluated, and a standardized grading system is provided for clinic. The method can solve the limitation of single omics analysis, improves the evaluation accuracy and reliability, has universality, can be popularized to other malignant tumor research, and assists precise medical development.

The invention relates to the technical field of artificial intelligence and biomedicine, and provides a multi-agent diagnosis planning device and method based on a consultation thinking process. The multi-agent diagnosis planning device comprises a diagnosis planning agent, a pathological section analysis agent, an in-hospital information aggregation agent, an information search agent, a knowledge base search agent, an evaluation agent, an arbitration agent and a risk assessment agent. Each agent realizes data interaction through a dynamic priority message bus, and the evaluation agent and the arbitration agent form a progressive verification closed loop: after the evaluation agent outputs a question evidence chain, the arbitration agent triggers diagnosis correction only when question items are greater than 3 items, and otherwise, final diagnosis is output based on a preset rule. According to the invention, the thinking process of multidisciplinary expert collaboration in clinical consultation is simulated by constructing a multi-agent collaborative diagnosis framework, and the whole process intelligence from medical data acquisition and analysis to diagnosis decision is realized.

The invention provides an IBS micro-ecological transplantation intelligent prediction method and system based on multi-omics driving, and relates to the technical field of biomedicine. The method comprises the following steps: establishing a multi-omics data fusion subsystem to collect metagenome, metabolome, host genome and clinical phenotype group data of a target patient; inputting the data into a flora-metabolite combined network analysis model to construct an interaction network and extracting features; generating an incidence matrix based on the features and the host genome data and calculating indexes; generating indexes through a dynamic response algorithm in combination with the clinical phenotypic data and the indexes; and outputting a curative effect prediction result by using a transfer learning framework combined with modeling. The system comprises a data acquisition module, a network analysis module, a correlation calculation module, a dynamic response module and a joint modeling module. According to the method, multiple omics data are integrated, the flora and host relation is accurately mined, intelligent prediction of the micro-ecological transplantation curative effect is achieved, powerful support is provided for IBS personalized treatment, and meanwhile data processing and safety guarantee measures are taken.

The invention belongs to the technical field of biological medicines, and particularly relates to application of secretory lactase-like protein (LCTL) as a diagnostic marker in preparation of a diagnostic kit for chronic kidney diseases. According to the present invention, the secretory form of the LCTL is found for the first time, the secretory LCTL content in the human serum is detected by using the existing commercial ELISA kit for the first time, and the further research finds that the secretory LCTL content in the serum of the chronic kidney disease patient is significantly reduced compared with the normal person; and the content of the LCTL in the serum is obviously related to the renal function impairment degree and proteinuria degree of the patient, so that the application of the secretory LCTL in preparing the biomarker for diagnosing the chronic kidney disease is provided for the first time. The invention provides a new method for chronic kidney disease diagnosis, and has important clinical significance and commercial value.

The invention discloses a macrocyclic covalent organic polymer material for inhibiting a pi-pi stacking effect and application of the macrocyclic covalent organic polymer material, and belongs to the technical field of biological medicines. The preparation method comprises the following steps: adding 5, 10, 15, 20-tetra (4-ethynylphenyl) copper porphyrin and a macrocyclic monomer into an organic solvent in a protective atmosphere, and carrying out solvothermal reaction in the presence of a catalyst and alkali to obtain the macrocyclic covalent organic polymer material. According to the invention, 5, 10, 15, 20-tetra (4-ethynylphenyl) copper porphyrin and a macrocyclic monomer form a porous polymer MCP-CU-POP, the material utilizes the photo-thermal capability to combine the effects of simulated photo-thermal (PTT), photodynamic (PDT), peroxidase (PODs) and glutathione peroxidase (GSH-Px) to perform synergistic antibiosis, and the inherent characteristics of an infected microenvironment can also be utilized to induce death of bacteria, so that the antibacterial property of the material is greatly improved, and the antibacterial property of the material is greatly improved. And the microenvironment of the bacterial infection part can be adjusted, so that the recovery of the bacterial infection wound can be obviously accelerated.

The invention relates to the technical field of biomedicine, in particular to a time interference dynamic multi-target accurate focusing transcranial electric therapeutic instrument which comprises a host module, an electric stimulation module, an intelligent stimulation mode optimization coordination module, an integrated electrode module, an electroencephalogram processing module, an adaptive treatment navigation module based on fMRI + EEG and a safety protection module. The navigation module calculates fMRI and EEG information, generates and optimizes a stimulation mode, the host module overall controls all the modules, the electrical stimulation module outputs multiple paths of high-frequency interference signals and low-frequency compensation signals, the integrated electrode module achieves stimulation and electroencephalogram collection in a time-sharing mode, the electroencephalogram processing module collects and processes the signals, and the safety protection module guarantees treatment safety. According to the application, the bottleneck that the current utilization rate is low, the positioning is fuzzy and the mode is rigid in the existing transcranial electrical stimulation technology is broken through, accurate synchronous / sequential stimulation of multiple deep brain regions is realized, and the accuracy, effectiveness and safety of treatment of neurological and mental diseases are improved.

This application relates to the field of biomedical technologies, and provides a data processing method and related devices. The method includes: collecting first data and second data from a patch clamp electrophysiological system, where the first data is data generated after a configured carbon fiber electrode applies multiple scanning voltages to a cell, and the second data is data generated after a configured stimulating electrode applies an electrical stimulation to the cell and then the configured carbon fiber electrode applies multiple scanning voltages to the cell; extracting target current data from the first data, and generating a first curve based on the target current data and the second data; sampling a single scanning voltage to obtain target sampling points, and generating a corresponding second curve based on the initial current data and the sampling time of the multiple target sampling points in the sampled first data or the sampled second data, so as to be able to obtain the original current and voltage data and convert the original data into an intuitive and effective curve.

The invention relates to the technical field of biomedicine, discloses mutant photo-induced ion channel ChR-2 protein, and further discloses a nucleic acid construct, an expression vector, a cell, related application and a computer model. The light-sensitive channel ChR2 protein mutant obtained by the invention has stronger light current, and the light-sensitive capability of the light-sensitive channel ChR2 protein mutant is at least improved by 100 times; besides, the invention also obtains a nucleotide sequence for coding the light-sensitive channel ChR2 protein mutant, constructs a recombinant expression vector, and obtains the light-sensitive channel ChR2 protein with higher expression quantity and stronger light sensitivity, and the light-sensitive channel ChR2 protein is very suitable for expression in cells of mammals (especially human); according to the invention, the rhodopsin in different channels is systematically studied by modifying the position G224 in the helix 6 of seven transmembrane helix motifs, which proves that the mutation of the position G224 in the helix 6 in WT ChR2 accelerates the photosensitivity of the channels, and the considered position is homologous in the helix 6 of the rhodopsin in different channels.

The invention provides an engineered membrane coated nano drug delivery system and application thereof, and belongs to the technical field of nano biomedices.The drug delivery system comprises an inner core combined with a drug, the inner core is coated with a cell membrane, based on the nano drug loading technology and the membrane coating technology, DOX and SRF are loaded on mPDA mesoporous nanoparticles, and thyroid cancer synergistic killing nanoparticles mPDS are formed; the bionic drug delivery system is characterized in that mPDS is used as a target drug delivery system, mPDS is coated with a genetically engineered tumor cell membrane, a dual-targeting membrane-coated nano drug delivery system (mPDS-coated CAR-M) is obtained, and the bionic drug delivery system can penetrate through thyroid cancer in a targeted manner, gather in a focus and reduce side effects of chemotherapy; when near-infrared irradiation is carried out on local tumor, DOX and SRF in mPDS are released, the synergistic effect of photothermal therapy-chemotherapy-targeted therapy is achieved, and the thyroid cancer is killed synergistically.

The invention relates to the technical field of biological medicines, in particular to rapamycin-induced extracellular vesicles and application thereof in preparation of a medicine for promoting wound repair. The rapamycin-induced extracellular vesicles can enhance the migration and tube forming ability of endothelial cells, so that angiogenesis is promoted; meanwhile, the proliferation and migration capabilities of macrophages can be inhibited, and the proportion of M1 type macrophages in a wound is reduced, so that the inflammatory response mediated by the macrophages is relieved; and the composition shows extraordinary effects in the aspect of accelerating wound repair. The biological activity of the extracellular vesicles induced by the rapamycin depends on the activation of a PI3K / Akt signal channel.

The invention provides a chronic hypoperfusion animal model construction method and application thereof, and relates to the technical field of biomedicine. The construction method of the chronic hypoperfusion animal model, provided by the invention, aims at brain capillary endothelial cells, and the constructed chronic hypoperfusion animal model is global and lasting brain hypoperfusion, has no infection or death risk and is low in cost. The animal model constructed by using the method is more convincing in the aspects of reperfusion research and drug curative effect evaluation.

The invention relates to the technical field of biomedicine, in particular to stem cell exosome-loaded bi-crosslinked hydrogel as well as preparation and application thereof, and the bi-crosslinked hydrogel comprises the following components in parts by weight: 50-90 parts of a matrix polymer; 0.1 to 5 parts of an active load; 1 to 10 parts of cyclodextrin; 5 to 20 parts of modified chitosan; 0.01 to 1 part of a photoinitiator; and 0.01 to 0.5 part of growth factors. Through double-network design of physical / chemical primary cross-linking and photo-initiation secondary cross-linking, the bottleneck that the mechanical property and the machinability of the traditional single cross-linking hydrogel are difficult to consider at the same time is overcome; meanwhile, the human umbilical vein endothelial cell exosome is introduced, and efficient loading, uniform dispersion and degradation coupling slow release of the exosome are realized by virtue of beta-cyclodextrin inclusion and a double-crosslinking network. Therefore, the problems of mechanics, degradation, cross-linking speed or tissue adhesion of cross-linked hydrogel in the prior art are solved.

The invention provides application of scEMC10 in treatment and diagnosis of cerebral vascular endothelial cell senescence, and relates to the technical field of biomedicine. The research of the inventor finds that brain microvascular endothelial cell senescence is caused by the lack of scEMC10; after the brain microvascular endothelial cells are treated by scEMC10, beta-galactosidase, SASP and classic senescence markers related to senescence can be down-regulated; after scEmc10 is supplemented to old mice and Alzheimer's disease 5XFAD model mice, the motion coordination ability and the spatial cognitive ability of the mice can be obviously improved, the relative content of a marker phosphorylated alpha-synuclein of the Parkinson's disease of the old mice can be obviously reduced, and the content of tyrosine hydroxylase phosphorylated active forms can be increased. Therefore, the scEMC10 can be used for treating brain microvascular endothelial cell senescence, improving Alzheimer's disease and Parkinson's disease and detecting brain microvascular endothelial cell senescence as a marker.

The invention discloses application of a stem cell exosome in treating depression-like behaviors caused by high-altitude hypoxia, and belongs to the technical field of biomedicine. The stem cell exosome is an exosome derived from human umbilical cord mesenchymal stem cells, and is used for drugs for preventing and / or treating depression-like behaviors caused by high-altitude hypoxia. The exosome can effectively improve depression-like core behavior symptoms caused by high-altitude hypoxia by regulating a brain-intestinal axis; the method starts from remodeling intestinal flora so as to influence the intracerebral metabolite spectrum, and finally plays a role in neuroprotection by regulating and controlling a 5-HT6 / cAMP-PKA key signal channel. In addition, the combination of the hUC-MSC-exo and probiotics shows synergistic interaction potential, and a potential treatment strategy which is novel in mechanism, remarkable in effect and high in safety is provided for the field.

The present invention relates to the field of biomedical technology, and more specifically to a method and system for assisting in predicting the risk of premature birth during pregnancy. The method comprises obtaining the physical data and delivery cycles of historical pregnant women, determining the degree of premature birth of the historical pregnant women based on the delivery cycles, and determining the degree of premature birth correlation of different types of physical data of the historical pregnant women based on the differences in the degree of premature birth between different historical pregnant women and the differences in the deviation changes of the same type of physical data during each physical examination. The historical pregnant women are clustered based on the deviation time differences of the same type of physical data between different historical pregnant women, and the overall correlation of each type of physical data is determined based on the number of pregnant women in the cluster and the degree of premature birth correlation of the corresponding type of physical data, thereby screening out the physical data of the target type. A premature birth prediction model is constructed based on the physical data of the target type during different physical examinations of the historical pregnant women to perform premature birth prediction. The present invention effectively improves the accuracy of premature birth prediction.

The invention discloses a lightweight multi-mode lower limb motion intention recognition method and system, and relates to the technical field of biomedicine. The method comprises the steps that multi-channel surface electromyogram signals sEMG of a target lower limb and joint angle signals and joint torque signals of lower limbs on the same side are synchronously collected; performing feature extraction by using a double-branch structure to obtain muscle-related deep time sequence features and joint-related high-level semantic features; performing feature fusion through a bidirectional cross attention mechanism to obtain cross attention fusion features; and performing flattening, nonlinear mapping, regularization and Softmax classification on the cross attention fusion features, and outputting the motion intention of the target lower limb. Through double-branch input, depth feature extraction and a bidirectional cross attention mechanism, on the premise of ensuring recognition precision, a lightweight attention module, a residual structure and a cross-modal interaction mechanism are introduced, and the parameter quantity and calculation overhead are reduced.

The invention relates to the technical field of orthopedic biomedical treatment, in particular to a proximal humerus fracture intramedullary bone cement supporting rod. The bone fracture plate comprises a bone fracture plate body, a plurality of first connecting holes and a plurality of second connecting holes are formed in one side of the bone fracture plate body in a penetrating mode, and solid bone nails are arranged in the first connecting holes. According to the device, bone cement is injected into a marrow cavity from an injection molding channel and an injection molding opening and then solidified into a rod body, the marrow cavity space is tightly filled, supporting structures are synchronously formed on the inner side and the outer side of the humerus through cooperation of a bone fracture plate and a bone cement supporting rod in the marrow cavity space, and therefore stress generated when arms of a patient move is evenly dispersed on the two sides of the humerus; the fixing stability is remarkably improved, the risk of internal fixation failure such as steel plate displacement and screw cutting is effectively reduced, and the bone cement supporting rods can further prevent displacement of fracture blocks, promote fracture repair, relieve extrusion and friction of surrounding tissue of a filling position, reduce inflammatory response and postoperative pain and improve the rehabilitation comfort of a patient.

The invention relates to the technical field of biomedicine, in particular to a method for constructing a plasma ctDNA organ distribution characteristic spectrum of advanced colorectal cancer. The method comprises the following steps: collecting a peripheral blood sample at multiple time points, separating plasma by adopting a double-centrifugal method, and extracting circulating tumor DNA (Deoxyribose Nucleic Acid); carrying out whole exome sequencing based on ctDNA to obtain genome variation information and calculating variation allele frequency, and synchronously detecting the expression quantity of immune-related proteins by adopting an Olink proteomics technology; integrating the genome data, the protein expression data and the clinical pathological features, and constructing a multi-dimensional feature data matrix; and taking the organ metastasis condition confirmed by iconography as a supervision label, training a model by applying a machine learning algorithm, screening key prediction factors, constructing a quantitative prediction model, and finally generating a visual organ metastasis tendency prediction map. According to the method, early and accurate prediction of the advanced colorectal cancer organ metastasis tendency is realized through multi-omics data collaborative analysis and machine learning modeling.

The invention belongs to the technical field of biological medicines, and particularly relates to application of 1, 3-propane diamine or pharmaceutically acceptable salts thereof in preparation of medicines for preventing or treating diabetes. Experimental results show that 1, 3-propane diamine can obviously improve random blood glucose and fasting blood glucose of mice with type 2 diabetes mellitus, and 1, 3-propane diamine can obviously improve insulin resistance of model mice. According to the application, 1, 3-propane diamine is used for improving the blood sugar of patients with type 2 diabetes for the first time, an ideal treatment effect is achieved, a new treatment choice is provided for improving the blood sugar level through clinical application of 1, 3-propane diamine, and the application has extremely high application value and social benefits.

The invention provides an application of narirutin in preparation of a medicine for preventing and / or treating osteoclast of inflammatory arthritis, relates to the technical field of biomedicine, and adopts a collagen-induced mouse arthritis model to observe the treatment effect of the narirutin on the osteoclast of the inflammatory arthritis. The arthritis activity degree of the mouse is analyzed through general claw photographing, thickness measurement, near-infrared temperature measurement and a claw imprinting experiment, three-dimensional reconstruction and bone trabecula analysis are carried out on local joints and the whole body bone mass of the mouse through micro-CT scanning, and Hamp is applied; e, dyeing and Famp; through histological staining analysis such as O staining and TRAP staining, in combination with immunohistochemical staining and the like, the narirutin is proved to play a role in treating inflammatory arthritis, inhibit macrophage pro-inflammatory M1 type polarization, relieve the inflammation level, improve the bone mass of local joints and the whole body, and further improve bone destruction in inflammatory arthritis diseases. The narirutin provides a new way for medicine prevention and treatment of inflammatory arthritis.

The invention relates to the technical field of biomedicine, in particular to application of gastrodin in prevention and treatment of Alzheimer's disease. The invention provides application of gastrodin in prevention and treatment of the Alzheimer's disease, and provides a new way for preventing and treating the Alzheimer's disease. And gastrodin is from gastrodia elata, is used as a mild medicinal and edible raw material component, and has no toxic or side effect on the body. Gastrodin is adopted as a raw material, and a series of experimental studies prove that gastrodin has an inhibitory effect on GSK-3beta / P38MAPK, and can obviously improve various pathological changes of the Alzheimer's disease caused by APOE4. The invention verifies the effect of gastrodin on the Alzheimer's disease high-risk factor APOE4, and provides a brand-new technical scheme and theoretical basis for developing medicines, health-care foods or nutritional supplements which take gastrodin as a core component and are used for preventing or treating Alzheimer's disease.

The invention is applicable to the technical field of biomedicine, and provides dipeptide-photosensitizer molecule co-assembled nanoparticles as well as a preparation method and application thereof. According to the invention, cationic diphenylalanine (CDP) and a biological cross-linking agent genipin are self-assembled to form a dipeptide carrier, and then the dipeptide carrier and a photosensitizer molecule chlorin e6 (Ce6) are co-assembled, so that nanoparticles with controllable size, controllable drug loading capacity and good biocompatibility are successfully prepared. The preparation method is simple and easy to implement, effectively solves the key problems that the traditional photosensitizer molecule Ce6 is poor in biocompatibility and easy to gather, not only can efficiently deliver Ce6, but also obviously improves the application safety and biocompatibility of Ce6. The Ce6 is almost free of dark toxicity under a dark condition, can play a strong photodynamic killing role under illumination, and greatly improves the possibility and application potential of the Ce6 in tumor photodynamic therapy by virtue of the flexible regulation and control advantages of the size and the drug loading capacity.

The present invention discloses the use of extracellular vesicles derived from oligodendrocytes carrying FTH1 in differentiating between multiple system atrophy and Parkinson's disease, belonging to the field of biomedical technology. The present invention utilizes fluorescently labeled CNPase antibodies and FTH1 antibodies to accurately detect extracellular vesicles derived from oligodendrocytes carrying FTH1 in plasma. By detecting the concentration of extracellular vesicles derived from oligodendrocytes carrying FTH1 (FTH1-positive) in plasma, it is possible to accurately distinguish between MSA patients, PD patients, and healthy individuals. This provides a highly effective biomarker for MSA diagnosis, improves the ability to diagnose MSA early and differentiate between MSA and PD, and effectively addresses the complexity and low sensitivity issues faced by traditional diagnostic methods.

The invention provides a culture method, application and detection method of mesenchymal stem cells for treating nervous system diseases, and belongs to the technical field of biological medicines. The invention provides a culture medium of mesenchymal stem cells, a method for culturing and subculturing the mesenchymal stem cells by using the culture medium, and a method for detecting the biological efficacy of the mesenchymal stem cells for treating nervous system diseases. The mesenchymal stem cell preparation disclosed by the invention has a relatively good treatment effect on nervous system diseases, and can be used for improving the new object recognition capability and the cerebral infarction range, inhibiting the release of inflammatory factors, reducing the oxidative stress level and narrowing the spinal cord lesion range so as to improve nervous system injury.

The invention belongs to the technical field of biomedicine, and particularly relates to application of GPR161 as an acute distress syndrome diagnostic marker and a therapeutic target. Experiments prove that the expression level of GPR161 in peripheral blood mononuclear cells of a patient with the acute respiratory distress syndrome is remarkably increased, and the expression quantity of the GPR161 is positively correlated with the severity of the disease, so that the GPR161 gene or GPR161 protein can be used as a molecular marker for screening or diagnosis or prognosis evaluation of the acute respiratory distress syndrome; a reagent for inhibiting GPR161 gene expression or protein activity can be used for preparing a medicine for treating the acute respiratory distress syndrome. The invention provides a new strategy for diagnosis, monitoring and targeted drug development of ARDS, and has important clinical application value.

The invention relates to 3D printing porous hydrogel based on a water-water emulsion as well as a preparation method and application of the 3D printing porous hydrogel, and relates to the technical field of biomedicine. The hydrogel is formed by photocuring crosslinking of a hydrogel precursor, the hydrogel precursor comprises a continuous phase medium and a dispersed phase medium, and the volume ratio of the continuous phase medium to the dispersed phase medium is 1: (2-5). The hydrogel provided by the invention breaks through the conventional volume ratio of a continuous phase medium to a dispersed phase medium, and breaks through the volume ratio limitation brought by a traditional water-water two-phase emulsion; the photocuring forming performance, the 3D printing forming performance, the swelling performance and the mechanical performance of the hydrogel are superior to those of existing hydrogel prepared from a high-volume continuous phase medium, so that a new technical system is provided for preparing hydrogel with higher porosity and connectivity.

The invention relates to the technical field of biomedicine, in particular to a mouse intrahepatic bile duct cancer cell strain and a construction method and application thereof, the cell strain is named as mouse intrahepatic bile duct cancer cell strain KP-ICC and preserved in China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: C202574; the cell strain is derived from a KrasG12D / Tp53flox / flox C57BL / 6J mouse, a spontaneous tumor model is constructed through high-pressure hydrodynamic tail vein injection of pT3-Cre and a sleep beauty transposase plasmid, and the spontaneous tumor model is obtained through in-vitro culture, passage, monoclonal screening and tumorigenicity verification. The KP-ICC cell strain naturally has chemotherapy-immune combined treatment drug resistance and ferroptosis resistance characteristics, and can be widely applied to the fields of intrahepatic cholangiocarcinoma drug resistance mechanism research, drug resistance reversing, combined treatment drug development, drug resistance animal model establishment and the like.

The invention relates to the technical field of biomedicine, in particular to a molecular target for treating recurrent refractory diffuse large B-cell lymphoma and application of the molecular target. According to the invention, a molecular target CSNK1E for treating recurrent refractory diffuse large B-cell lymphoma is excavated; compared with existing traditional targets of other drugs for recurrent lymphoma, the CSNK1E has a remarkable expression difference in the recurrent DLBCL, and it is indicated that the CSNK1E possibly has more remarkable advantages in treatment of the recurrent DLBCL.

The invention relates to a method for promoting nerve cell proliferation and differentiation based on ultrasonic stimulation and application, and belongs to the technical field of biomedicine. According to the method, under the conditions that the power is 90-110 mW and the frequency is 0.9-1.1 MHz, nerve cells are continuously stimulated for 3 days by ultrasonic waves once per day, and each time lasts for 2-4 min. The purposes of increasing the nerve cell proliferation speed and promoting nerve cell differentiation are achieved only through ultrasonic stimulation under specific conditions for the first time, no other substances are added, non-invasion and non-contact are achieved, and toxic and side effects on cells are low; the nerve cells are not subjected to invasive or modified operation; the method has a wide application prospect only through physical conditions.