559 results about "Fibroin" patented technology

The invention relates to a stable silk fibroin liquid condensation body, gel and a preparation method and application thereof, and belongs to the technical field of silk fibroin. The preparation method comprises the following steps: S1, dissolving silk fibroin in a lithium bromide solution, and then adding trypsin for incubation to obtain an enzyme-digested silk fibroin solution; and S2, performing low-temperature programmed centrifugal treatment including an induction stage, a nucleation stage and a liquid-liquid phase separation stage on the enzyme-digested silk fibroin solution, and cleaning to obtain the stable silk fibroin liquid-state aggregate, the method can reduce aggregate liquid drop aggregation and improve the particle size uniformity of the liquid drops.

The invention discloses a silk protein-MXene composite film and a preparation method thereof. The silk protein-MXene composite film sequentially comprises a conductive surface layer, a porous response layer and a supporting bottom layer from top to bottom, the invention relates to the technical field of composite films. According to the fibroin-MXene composite film and the preparation method thereof, a three-layer gradient structure is formed by the conductive surface layer, the porous response layer and the supporting bottom layer, good conductivity and electromagnetic shielding effectiveness are ensured by utilizing oriented arrangement of MXene of the conductive surface layer, gallic acid and MXene form a hydrogen bond network, and nitrogen-doped carbon quantum dots coat the surface of MXene to form a physical barrier, so that the electromagnetic shielding effect is improved, and the electromagnetic shielding effect is improved. Through double oxidation resistance of gallic acid and nitrogen-doped carbon quantum dots, a sponge body structure formed by the porous response layer is matched to endow the composite film with high-sensitivity response to external stimulation, the supporting bottom layer is crosslinked by hexamethylene diisocyanate and enhanced by nanocellulose, the tensile strength is improved, and the tensile strength of the composite film is improved. And the contradiction between the mechanical property and the conductivity of the traditional composite film is effectively solved.

The invention belongs to the technical field of cosmetic preparation, and particularly relates to a skin moisturizing and repairing composition based on dendrobium officinale and fibroin as well as a preparation method and application of the skin moisturizing and repairing composition. Essence extracted from dendrobium officinale tender shoots obtained through a plant stem cell culture technology and stepped molecular weight fibroin prepared through a composite enzymolysis technology serve as raw materials, the safe and efficient composition is developed, and the actual requirements for skin moisturizing and repairing are met.

The invention discloses uniform-particle-size silk fibroin microspheres and a preparation method and application thereof.The preparation method comprises the following steps that a regenerated silk fibroin solution is taken and subjected to trypsin digestion to obtain modified silk fibroin sediment, and the modified silk fibroin sediment is dissolved in an aqueous solution of a beta-folding inhibitor to obtain a silk fibroin solution serving as a dispersed phase; emulsifying through an external pressure type membrane emulsifier to obtain an emulsion containing the silk fibroin emulsion droplets; adding a cross-linking agent into the emulsion to carry out cross-linking reaction, and then adding a crystallization inducer to induce crystallization to obtain primary silk fibroin microspheres; and performing vacuum freeze drying to obtain the silk fibroin microspheres. Silk fibroin is subjected to enzyme digestion modification, silk fibroin fragments with high beta-folding content are reserved, and high structural stability is achieved; the crystallinity of the microspheres is improved by adopting a membrane emulsification mode and combining a multiple crystallization induction method, and the silk fibroin microspheres with uniform particle size and smooth surfaces are obtained.

The invention relates to conductive silk fibroin loaded methacrylated gelatin hydrogel and a preparation method thereof, and belongs to the technical field of biomedical materials. The preparation method comprises the following steps: S1, adding silk fibroin fibers into a dopamine hydrochloride solution, stirring, standing, then adjusting the pH value to 8-9, and carrying out a heating reaction to obtain polydopamine grafted silk fibroin fibers; s2, sequentially dipping the polydopamine grafted silk fibroin fibers in an ammonium persulfate solution and a pyrrole solution to obtain conductive silk fibroin fibers; and S3, adding the conductive silk fibroin fibers into the methacrylated gelatin solution, carrying out ultraviolet light cross-linking curing, and then soaking the conductive silk fibroin fibers in a glycerol solution to obtain the conductive silk fibroin loaded methacrylated gelatin hydrogel. The method not only can ensure the structural integrity and conductive continuity of a conductive network in the hydrogel, but also breaks through the dependence of in-situ polymerization on the permeability of a matrix.

The invention discloses silk fibroin-sodium alginate-chitosan bionic leather and a preparation method and application thereof.The preparation method comprises the following steps that a silk fibroin solution and a sodium alginate solution are mixed, after calcium salt is added, a chitosan solution continues to be added and mixed with waterborne polyurethane, and a mixed solution is obtained; and carrying out surface activation treatment on base cloth, coating the activated base cloth with the mixed solution, and carrying out drying curing and ultrasonic lamination to obtain the silk fibroin-sodium alginate-chitosan bionic leather. The silk fibroin, sodium alginate and chitosan bionic leather prepared by the invention not only has high strength and good ductility, but also has excellent tensile strength, folding resistance and wear resistance, and also has good biodegradability, so that the burden of waste post-treatment on the environment can be reduced, and the raw material cost and post-treatment cost of the bionic leather can be remarkably reduced; the method has both environmental benefits and outstanding economic benefits.

The invention provides a silk fibroin-based physical microgel material and a preparation method thereof, and belongs to the field of biomedical materials. According to the preparation method, an organic solvent is added into a silk fibroin solution, micron-sized gel particles with different sizes and different silk fibroin contents can be obtained by regulating and controlling the water-oil flow rate ratio and the silk fibroin concentration through the microfluidic technology, and the organic solvent and polysaccharide can be added into the silk fibroin solution at the same time; and the polysaccharide-silk fibroin mixed microgel is obtained through a microfluidic technology. The method is simple to operate and cost-saving, not only accelerates the sol-gel conversion speed of the silk fibroin, reduces the viscosity of the solution to enable the solution to pass through the micro-fluidic chip more easily, but also can promote the stability of the mixed solution of the silk fibroin and the polysaccharide, improves the mechanical property of the silk fibroin microgel and improves the stability of the micro-fluidic chip. The obtained silk fibroin-based microgel is uniform in size, regular in shape and high in porosity.

The invention discloses a high-strength microneedle with directional holes for biological analysis sampling and a preparation method of the high-strength microneedle, and belongs to the technical field of microneedles, the method comprises the following steps: filling a silk fibroin solution into a microneedle mold to form a microneedle preform, the microneedle preform comprising a needle body part and a backing part; performing directional freezing on the microneedle preform to enable ice crystals to directionally grow in the direction from the backing part to the needle body part, and demolding to obtain a frozen blank; the frozen blank is placed in an organic solution of a cross-linking agent, soaked at the low temperature lower than 0 DEG C for ice template low-temperature solvent exchange-cross-linking treatment, taken out and cleaned, then soaked in a salt solution for salting-out treatment strengthening, taken out and cleaned and freeze-dried, and the high-strength microneedle with the directional holes is obtained. The microneedle prepared through the method has high strength and excellent puncture performance, and the skin interstitial fluid collection speed and efficiency are greatly improved.

The invention discloses an antibacterial dressing for promoting wound repair and regeneration and a preparation method thereof, and the preparation method comprises the following steps: adding hydrogen peroxide into a silk fibroin aqueous solution to prepare a silk fibroin aqueous solution premixed with hydrogen peroxide; horse radish peroxidase is added into the dopamine modified sodium alginate solution, and the dopamine modified sodium alginate solution premixed with horse radish peroxidase is prepared. Preparing a chondroitin sulfate composite solution; uniformly mixing the silk fibroin aqueous solution premixed with hydrogen peroxide, the dopamine modified sodium alginate solution premixed with horse radish peroxidase and the chondroitin sulfate composite solution. Hydrogel formed by cross-linking silk fibroin and chondroitin sulfate is used as a main body material of the dressing, and meanwhile, dopamine modified sodium alginate is introduced, so that the dressing has the photo-thermal antibacterial characteristic; magnesium oxide microspheres are introduced as a pore-foaming agent, micropores are formed in the dressing, and the liquid absorption property and the air permeability of the dressing are improved; the umbilical cord mesenchymal stem cell exosome is introduced, so that angiogenesis at the wound is promoted, and the wound healing promoting capability of the dressing is improved.

Provided are chemically modified silk fibroin and a use thereof in cell and organoid culture. The chemically modified silk fibroin is a product obtained by sequentially carboxylating silk fibroin and modifying same by an organic amine having a phenol group. Also provided is a hydrogel based on the chemically modified silk fibroin. A hydrogel system has a hierarchical structure and mechanical properties similar to those of an extracellular matrix, and exhibits characteristics such as definite composition, controllable physicochemical properties, low cytotoxicity, good biocompatibility, and biodegradability, can support the growth and differentiation of cells and organoids, and is suitable as a matrigel for cell and organoid culture.

The invention discloses a silk fibroin solution injection capable of forming gel in situ and a preparation method and application thereof.The preparation method comprises the following steps that a regenerated silk fibroin solution with the molecular weight of 100 kDa-250 kDa and a hydrogen bond breaker are evenly mixed to obtain a mixed solution, the mixed solution is subjected to quick-freezing supercooling crystallization and vacuum drying to obtain silk fibroin powder, and the silk fibroin powder is dried to obtain the silk fibroin solution injection capable of forming gel in situ. And sterilizing and dissolving in normal saline to obtain the silk fibroin solution injection. A hydrogen bond breaker is introduced into a high-molecular-weight regenerated silk fibroin solution, a beta folding structure between silk fibroin molecules is inhibited, damage to the silk fibroin molecules in the freeze-drying process is reduced through a quick-freezing and supercooling crystallization method, and aggregation of the silk fibroin molecules is further reduced; after redissolving, an injection is formed and injected into local tissues, the micromolecular hydrogen bond breaker quickly diffuses, and the large molecular weight regenerated silk fibroin recovers hydrogen bond interaction and then forms hydrogel in situ through physical crosslinking, so that in-vivo in-situ gelling of the silk fibroin is realized.

The invention provides a dual-network hyaluronic acid-silk fibroin gel sponge and a preparation method thereof, and belongs to the technical field of gel sponge materials. The preparation method comprises the following steps: mixing sulfhydrylation modified hyaluronic acid and a silk fibroin solution, and sequentially performing freezing and soft freezing treatment to form the hyaluronic acid-silk fibroin gel sponge with a good shape. In the freezing induction process, hyaluronic acid generates chemical crosslinking through disulfide bonds, silk fibroin forms physical crosslinking through freezing concentration, and finally the stable gel-sponge coexistence body with the double-network structure is formed. The gel sponge has double advantages of gel and sponge. Meanwhile, due to freeze concentration treatment, the gel sponge has a macroporous structure, which is beneficial to potentially promoting adhesion, proliferation and migration of cells; the prepared gel sponge does not introduce additional chemical reagents, the biological safety of the gel sponge is guaranteed, and the gel sponge is suitable for the fields of cartilage / skin repair, tissue filling, wound dressing and the like.

The application discloses sulfonated silk protein gel as an interface modification layer of a water-based zinc electrode and a preparation method thereof, and belongs to the technical field of negative electrode materials of zinc ion batteries. The sulfonated silk protein gel is prepared by using natural silk, raw materials are widely sourced, and the sulfonated silk protein gel is green and environment-friendly. The prepared sulfonated silk protein gel contains rich functional groups (‑OH, ‑NH‑, ‑C=O, ‑SO3H and the like), can cover the entire zinc electrode surface, forms a gel-like transition zone at the interface, and provides basic protection for the zinc electrode. In addition, the gel layer optimizes the electric field, improves the interface compatibility, can guarantee uniformity in the nucleation and growth process, plays an obvious role in controlling the dendrite growth, effectively prolongs the service life of the zinc ion battery, and improves the cycle stability of the zinc ion battery.

The invention provides a method for detecting the contents of collagen and regenerated silk fibroin in a composite material, which comprises the following steps: (1) hydrolyzing the composite material for a certain time, and neutralizing to obtain a solution to be detected; (2) preparing a tyrosine reference solution; and (3) detecting the peak areas of the solution to be detected and the tyrosine reference substance solution by adopting high performance liquid chromatography, and calculating to obtain the content of regenerated silk fibroin in the composite material, and (4) detecting the total nitrogen content of the composite material by using a Kjeldahl method, and calculating to obtain the collagen content in the composite material. According to the method, the high performance liquid chromatography is combined with the Kjeldahl determination method, accurate determination of two components can be realized without derivatization treatment, the operation is simple and convenient, and the detection cost is low; the limit of quantitation of the method reaches 10.35 mu g / mL (S / N is greater than or equal to 16.59), the precision RSD is less than 1%, and the method has high sensitivity and accuracy.

The invention relates to a preparation method of a biological membrane with a bionic cuticle'brick-slurry 'structure and a wound dressing application of the biological membrane, and belongs to the technical field of biological auxiliary materials. The preparation method comprises the following steps: dispersing prepared wool keratin microspheres in a fibroin solution, realizing a stable and firm brick-slurry structure by combining solvent volatilization with a photo-crosslinking technology, further crosslinking by utilizing metal ion coordination, and simultaneously endowing the dressing with the capabilities of effectively blocking bacterial permeation and sterilizing. The biological membrane exceeds the design of traditional layered wound dressings, and accurate reproduction of a natural skin structure is preferentially considered. The microspheres are assembled into a densely-arranged masonry structure material in a planned manner, so that the dressing has high moisture permeability, oxygen permeability and strong antibacterial barrier capacity, can promote repair of infectious wounds and burn and scald wounds, and has remarkable clinical application value.

The invention relates to the technical field of flexible electronic materials, and discloses a silk fibroin-based topological structure conductive hydrogel and a preparation method and application thereof.The silk fibroin-based topological structure conductive hydrogel is prepared from rGO-coated SilMA serving as a polymerization main body, the rGO-coated SilMA is formed by mixing silk fibroin and graphene oxide dispersion liquid and reacting in the presence of methacrylic acid glyceride, and the rGO-coated SilMA is prepared through the steps that the rGO-coated SilMA serves as the polymerization main body; according to the invention, rGO (at) SilMA is taken as a substrate, polyethylene glycol diacrylate is taken as a reinforcing phase, phenyl-2, 4, 6-trimethylbenzoyl lithium phosphate (LAP) is taken as a photoinitiator, and the mass ratios of all the components in the photocuring ink are as follows: 20% of rGO (at) SilMA, 10% of PEGDA and 0.75% of LAP. The rGO-coated SilMA-PEGDA interpenetrating network structure is constructed and TPMS topological design is introduced, so that the mechanical property and sensing stability of the hydrogel are remarkably improved, the compression modulus is improved by the interpenetrating network, the elongation at break is kept at a high level, the high-deformation scene requirement is met, the stress distribution is more uniform by the negative Gaussian curvature characteristic of the topological structure, and the effective sensing area is increased.

The invention belongs to the technical field of cosmetics, and particularly relates to a silk fibroin aggregate-based dispersed organic active substance as well as a preparation method and application thereof. The preparation method of the silk fibroin aggregate-based dispersed organic active substance comprises the following steps: (1) dissolving an organic active substance in a first solvent to obtain a first solution; dissolving the silk fibroin aggregate in a second solvent to obtain a second solution; (2) slowly adding the second solution into the first solution, mixing and homogenizing to obtain a silk fibroin aggregate-based dispersed organic active substance; wherein the silk fibroin aggregate is prepared by mixing a silk fibroin aqueous solution and alcohol. The silk fibroin aggregate-based dispersed organic active substance prepared by the preparation method can improve the bioavailability of the organic active substance (such as vitamin C, hyaluronic acid, coenzyme Q10, nicotinamide, arbutin, kojic acid, licorice extract, centella extract, chamomile extract or green tea extract) in cosmetics.

The invention belongs to the technical field of biological materials, and particularly relates to a long-term stable silk fibroin solution and a preparation method thereof, and the preparation method comprises the following steps: (1) dissolving a composite stabilizer in water, and uniformly mixing to obtain a stable solution; wherein the compound stabilizer comprises a micromolecular hydrogen bond chelating agent and a macromolecular steric hindrance agent; (2) freeze-drying and dissolving silk fibroin in the stabilizing solution to obtain a silk fibroin solution; and (3) sterilizing the silk fibroin solution. By utilizing the synergistic effect of the micromolecular hydrogen bond chelating agent and the macromolecular steric hindrance agent, the stabilizing effect can be adjusted according to the storage environment, and the stable silk fibroin solution can be stored at 4-7 DEG C for a long time (more than or equal to 6 months) and can be stored at room temperature for more than or equal to 3 months.

The invention discloses a friction nano-generator based on silk fibroin composite aerogel, a preparation method and application of the friction nano-generator. Biomass SF serves as a matrix and is combined with high-conductivity MXene, an interface structure between the MXene and the SF is improved through sodium alginate (SA), and biomass conductive aerogel with high mechanical property and electrical property is constructed and serves as a positive friction material; the friction nano-generator, which is combined with a polydimethylsiloxane / barium titanate (PDMS / BaTiO3) composite film with high dielectric property as a negative friction material, has high frictional electricity output property and stability. According to the invention, three different types of friction nano-generators of a plane type, a folding type and a wave type are developed, and the wave type friction nano-generator has the most excellent high-friction electricity output property and stability.

The invention discloses an absorbable suture line containing fibroin and a preparation method of the absorbable suture line, and relates to the technical field of absorbable suture lines. The absorbable suture line containing the silk protein is prepared from the following raw materials in parts by weight: 35 to 50 parts of silk protein, 10 to 20 parts of polylactic acid, 15 to 25 parts of polyglycolide-lactide, 1 to 3 parts of antibacterial agent, 2 to 5 parts of modified toughening agent, 1 to 4 parts of nano mineral filler, 0.5 to 2 parts of cross-linking agent and 2 to 5 parts of plasticizer, the preparation method comprises the following steps: reacting protocatechuic acid with ethanolamine to generate an intermediate 1; and reacting the intermediate 1 with 5, 10, 15, 20-tetra (4-carboxyl phenyl) porphyrin to generate the antibacterial agent. The prepared absorbable suture line containing the silk protein has good mechanical performance, self-repairing performance and antibacterial performance.

The invention relates to composite aerogel as well as a preparation method and application thereof, and belongs to the technical field of bio-based materials, the composite aerogel is prepared from the following components in parts by mass: 2-10 parts of aldehyde modified cellulose nanocrystals, 3-10 parts of a silk fibroin solution, 5-15 parts of a genipin ethanol solution and 0.2-0.5 part of zinc chloride; the preparation method of the composite aerogel comprises the following steps: (1) uniformly mixing aldehyde modified cellulose nanocrystals, a silk fibroin solution, a genipin ethanol solution and zinc chloride, and reacting to form hydrogel; (2) adding into a mold, and freezing at constant temperature for 4-8 hours; (3) replacing water with tert-butyl alcohol; and (4) drying at normal pressure for 24-48 hours to obtain the composite aerogel. The aerogel prepared by the invention has a hierarchical pore structure and an adjustable function, has high mechanical properties, specific surface area, porosity and degradation rate, and is suitable for the fields of biomedicine, environmental protection and energy.

The invention discloses a water-soluble fibroin-novel indole cyan green nano particle and a preparation method and application thereof, biocompatible silk fibroin and small photo-thermal molecules of novel indole cyan green dye are integrated together, and the water-soluble fibroin-novel indole cyan green nano particle expressed as SF (at) IR820 is obtained. The SF (at) IR820 prepared by the invention can go deep into tumor cells. The SF (at) IR820 can effectively eliminate 4T1 tumor cells in vitro and inhibit tumor growth in vivo when exposed to 808nm laser radiation, a promising strategy is provided for developing an efficient photothermal agent for cancer treatment, and the SF (at) IR820 has certain reference significance and application value.

The application provides a silk fibroin microsphere and a preparation method and application thereof, and belongs to the technical field of biopolymer microsphere preparation. The preparation method comprises the following steps: 1) adding an ionic dispersing agent into a silk fibroin solution, uniformly mixing, then adding a sphere forming agent into the solution, and obtaining a pretreated liquid after mixing; 2) performing low-temperature coagulation treatment on the pretreated liquid, and obtaining a silk fibroin microsphere solution after thawing; 3) diluting the silk fibroin microsphere solution, then performing wet heat crystallization treatment, and obtaining a high-crystallinity silk fibroin microsphere solution; and 4) performing spray drying on the high-crystallinity silk fibroin microsphere solution, and obtaining a solid silk fibroin microsphere. The silk fibroin microsphere obtained by the application has excellent biocompatibility, degradability and good mechanical properties. The silk fibroin microsphere prepared by the method of the application is regular spherical, has high crystallinity, slow degradation speed, is not easy to absorb moisture and adhere, and can be stored for a long time; and the preparation method is simple and has high yield.

The invention relates to the technical field of building materials, and discloses a mortar containing a nanometer material and a preparation method thereof, the mortar comprises 70-80 parts by weight of cement, 8-12 parts by weight of fly ash, 0.4-0.5 part by weight of a polycarboxylic acid water reducer, 0.4-0.6 part by weight of hydroxypropyl methyl cellulose, 1-2 parts by weight of the nanometer material, 2-3 parts by weight of modified silk fibroin and 40-50 parts by weight of water. Wherein the modified nano silicon dioxide is modified by gamma-aminopropyltriethoxysilane, the modified cubic nano calcium carbonate is subjected to compound modification by fumaric acid, sodium stearate and palmitic acid, and the modified silk fibroin is prepared by grafting modification by bis-aminopropyl terminated polysiloxane. According to the mortar, through optimization of component matching and modification treatment of the nano material and the silk fibroin, the mechanical property, durability and crack resistance of the mortar are remarkably improved, and the cost is controllable.

The invention discloses a preparation method of silk fibroin-based hemostatic gel. The preparation method comprises the following steps: (1) hydrolyzing regenerated silk fibroin, and dialyzing to obtain micromolecular silk fibroin; (2) modifying the micromolecular silk fibroin by using a modifier to obtain micromolecular modified silk fibroin; and (3) mixing the small-molecule modified silk fibroin with an auxiliary agent, a photoinitiator, modified gelatin and raspberry ketone glucoside in proportion, and stirring and reacting in a dark place to obtain the silk fibroin-based hemostatic gel. The silk fibroin-based hemostatic gel prepared by the invention has the advantages of rapid hemostasis, good application property and the like, and can be widely applied to the fields of trauma hemostasis and the like.

The invention discloses a gelatin-silk fibroin composite drug-loaded nanoparticle, which is characterized in that gelatin and silk fibroin are co-assembled through physical crosslinking to form a stable composite structure, the silk fibroin forms a physical crosslinking network through a beta-folding structure, and the gelatin is distributed in the network. The invention further discloses a preparation method of the nano-particles and application of the nano-particles in preparation of drugs for treating inflammatory diseases. The nanoparticle does not need a chemical cross-linking agent, has the characteristics of controllable particle size, high drug loading capacity, high encapsulation efficiency and MMP-9 enzyme response drug release, has good biocompatibility and anti-inflammatory effect, and can be used for treating inflammatory diseases.

The invention discloses a motion monitoring clothing system integrating nano sensing and edge intelligence, which comprises three innovative modules: 1, a nano sensing network: adopting MXene / fibroin composite nano cobweb structure sensors (response sensitivity reaches 2.5 kPa), arranging the sensors in a clothing joint area in a bionic muscle texture manner, and capturing micro strain (0.1-15%) and three-dimensional attitude in real time; 2, a self-adaptive learning engine: carrying a lightweight graph convolutional neural network (GCN), generalizing motion data of athletes in a certain winter sports event into a general model through transfer learning, and realizing that the motion recognition accuracy is greater than or equal to 98.7%; the dynamic feedback system is used for generating tactile prompts through a micro-pneumatic actuator array and correcting dangerous postures. The system breaks through the rigid limitation of traditional exercise monitoring equipment, the stretch rate exceeds 180%, the exercise injury rate can be reduced by 42% according to actual measurement of a professional speed team, and the system has been applied to the fields of competitive training, rehabilitation medicine and the like.

A composite membrane comprises a membrane matrix formed by the stable cross-linking of silk fibroin with β-sheets, and baicalin uniformly dispersed between the segments of the silk fibroin molecular chain, wherein the mass ratio of silk fibroin to baicalin is 100:3-4. The preparation method includes the following steps: 1) taking silkworm cocoons, boiling them several times with sodium carbonate solution, washing them, and drying them to 60°C; 2) dissolving them in a ternary solution, centrifuging and filtering to obtain a clear silk fibroin solution, and drying it to obtain silk fibroin; 3) preparing a silk fibroin solution with a concentration of 1-30 mg / ml, and adjusting the pH to 7.4; 4) adding baicalin to the silk fibroin solution, forming a film, drying it, and annealing it to obtain the composite membrane. The composite membrane of this invention has superior biocompatibility, hemostatic properties, and controllable biodegradability, and can promote M2 polarization of macrophages, shorten the inflammatory period, and effectively promote skin wound healing.

The invention provides a preparation method and application of a near-infrared light induction type silk fibroin nano preparation, and belongs to the technical field of medical preparations. And dropwise adding an ICG solution into the silk fibroin nano-particle solution, and then adding a ferric trichloride solution for reaction to obtain a precipitate, namely the near-infrared light induction type silk fibroin nano-preparation. The nano preparation prepared by the method disclosed by the invention can realize synergistic interaction of two treatment mechanisms, and the anti-tumor effect is remarkably improved.

The invention provides porous silk fibroin microspheres as well as a preparation method and application thereof, and belongs to the technical field of biomedical materials. According to the method, a high-molecular compound containing a hydrophilic functional group is selected as a specific inducer, a silk fibroin solution and the specific inducer are blended, the intermolecular interaction of silk fibroin is regulated and controlled under the action of the inducer, and a large number of silk fibroin microspheres which are uniform in size and stable in structure are formed in a self-assembly mode in the concentration-film forming process. On the basis, the inducer is removed, and then drying treatment is performed to form the porous silk fibroin microspheres with high purity and high specific surface area. The preparation process is simple, no toxic reagent is used in the preparation process, and the obtained silk fibroin microspheres can be applied to the fields of cell culture carriers, drug delivery systems, protein separation and purification and the like and have wide application prospects in the field of biomedical materials.