787 results about "Fibroin" patented technology

The invention discloses injectable sodium hyaluronate-silk fibroin composite hydrogel and a preparation method and application thereof.The injectable sodium hyaluronate-silk fibroin composite hydrogel is composed of sodium hyaluronate composite gel particles and a sodium hyaluronate solution, and the sodium hyaluronate composite gel particles comprise silk fibroin sponge microspheres and sodium hyaluronate gel particles; the silk fibroin sponge microspheres are loaded on the sodium hyaluronate gel particles, the silk fibroin sponge microspheres are physical crosslinking microspheres, the particle size D50 is 10-30 [mu] m, and the particle size D90 is within 100 [mu] m; the sodium hyaluronate gel particles are chemical cross-linked particles, the particle size D50 is 150-300 microns, and the particle size D90 is within 800 microns. After the sodium hyaluronate gel particles are in contact with tissues, sodium hyaluronate is preferentially degraded, and silk fibroin exposed in the degradation process has better degradation resistance and an effect of stimulating generation of collagen fibers, so that in-situ fixation of the composite gel particles is realized, and an instant and lasting filling effect is achieved; the material is a safe and effective medical beauty filling material.

The invention discloses silk fibroin composite gel and a preparation method thereof, and relates to the technical field of tissue filling materials. The preparation method of the silk fibroin composite gel comprises the following steps: preparing silk fibroin and carrying out amination modification on the silk fibroin to obtain aminated silk fibroin; carrying out dynamic cross-linking on the aminated silk fibroin and the oxidized sodium alginate through a Schiff base bond by adopting an enzyme catalysis cross-linking process to obtain a dynamic cross-linked network solution; adding a temperature-sensitive component into the dynamic cross-linked network solution, and uniformly mixing to obtain a temperature-sensitive-dynamic dual-network pre-gel solution with temperature responsiveness; loading the active component into the temperature-sensitive-dynamic dual-network pregel solution to obtain a composite gel solution; incubating the composite gel solution to obtain primary gel; after disulfide bonds are introduced into the primary gel, cross-linking strengthening and sterilization are carried out, and the silk fibroin composite gel is obtained. Through temperature-sensitive-dynamic dual-network design, active components and disulfide bonds are introduced to strengthen crosslinking, and the problems that traditional injectable gel is irreversible, single in function, prone to collapse after being filled and the like are systematically solved.

The invention relates to a stable silk fibroin liquid condensation body, gel and a preparation method and application thereof, and belongs to the technical field of silk fibroin. The preparation method comprises the following steps: S1, dissolving silk fibroin in a lithium bromide solution, and then adding trypsin for incubation to obtain an enzyme-digested silk fibroin solution; and S2, performing low-temperature programmed centrifugal treatment including an induction stage, a nucleation stage and a liquid-liquid phase separation stage on the enzyme-digested silk fibroin solution, and cleaning to obtain the stable silk fibroin liquid-state aggregate, the method can reduce aggregate liquid drop aggregation and improve the particle size uniformity of the liquid drops.

The invention relates to a silk fibroin biological material with high beta-fold content and a preparation method thereof, and belongs to the technical field of biological extraction. The method comprises the following steps: preparing a regenerated silk fibroin solution; adding an enzyme catalytic promoter and an enzyme into the regenerated silk fibroin solution for incubation; the enzyme catalysis auxiliary agent is selected from one or more of a metal ion catalyst, a zwitterionic surfactant, a functionalized ionic liquid and a calcium phosphate nano-cluster; and centrifuging, cleaning and drying the obtained product to obtain the silk fibroin biological material with high beta-fold content. The silk fibroin material has higher beta-folding amount through primary structure modification, the space structure is more crystallized, and the amino acid sequence is more regular and ordered. The silk fibroin material provided by the invention has excellent mechanical properties, efficiency and yield, and is a biological protein material with great development potential.

The invention provides a silk fibroin hydrogel preparation method, which comprises: S1, dissolving degummed silk in a neutral salt solution to obtain a silk fibroin dissolving solution; s2, diluting the silk fibroin dissolving solution prepared in the step S1 with a diluent to obtain a silk fibroin dissolving diluent; s3, carrying out physical cross-linking treatment on the silk fibroin dissolving diluent prepared in the step S2 or regulating the pH value for physical cross-linking or adding a surfactant for physical cross-linking, and incubating to obtain the silk fibroin hydrogel. The silk fibroin hydrogel is simple in preparation process, high in gelling rate, low in loss and high in raw material utilization rate.

The present invention relates to the field of biomedical materials. Particularly provided is a DNA-silk fibroin composite hydrogel microsphere. The DNA-silk fibroin composite hydrogel microsphere is prepared from DNA and silk fibroin on the basis of the micro-fluidic technology by means of combination of self-assembly physical crosslinking and photo-crosslinking. The introduction of a silk fibroin network into the composite hydrogel microsphere can provide mechanical performance for a DNA hydrogel network and reduce the production cost. RGD can remarkably improve the cell loading capability of the microsphere. The hydrogel microsphere can simulate a microenvironment of cartilage tissue by means of three-dimensional loading of high-density cells, so as to enhance substance exchange and signaling between cells, and more effectively promote the proliferation and adhesion of bone marrow mesenchymal stem cells and regulating the chondrogenic differentiation, thus promoting cartilage regeneration and repair, and providing an available material for preparing medicaments or medical instruments for cartilage defect repair.

The invention discloses a silk protein-MXene composite film and a preparation method thereof. The silk protein-MXene composite film sequentially comprises a conductive surface layer, a porous response layer and a supporting bottom layer from top to bottom, the invention relates to the technical field of composite films. According to the fibroin-MXene composite film and the preparation method thereof, a three-layer gradient structure is formed by the conductive surface layer, the porous response layer and the supporting bottom layer, good conductivity and electromagnetic shielding effectiveness are ensured by utilizing oriented arrangement of MXene of the conductive surface layer, gallic acid and MXene form a hydrogen bond network, and nitrogen-doped carbon quantum dots coat the surface of MXene to form a physical barrier, so that the electromagnetic shielding effect is improved, and the electromagnetic shielding effect is improved. Through double oxidation resistance of gallic acid and nitrogen-doped carbon quantum dots, a sponge body structure formed by the porous response layer is matched to endow the composite film with high-sensitivity response to external stimulation, the supporting bottom layer is crosslinked by hexamethylene diisocyanate and enhanced by nanocellulose, the tensile strength is improved, and the tensile strength of the composite film is improved. And the contradiction between the mechanical property and the conductivity of the traditional composite film is effectively solved.

The invention provides double physical cross-linked silk fibroin composite gel as well as a preparation method and application thereof. A silk fibroin solution is cross-linked twice in a first cross-linking agent and a second cross-linking agent, the first cross-linking agent and the second cross-linking agent are hydroxyl cellulose or carboxyl cellulose or corresponding derivatives, the first cross-linking agent induces beta-folding in silk fibroin, the second cross-linking agent induces beta-folding among silk fibroin molecules, and the second cross-linking agent induces beta-folding among silk fibroin molecules. The first cross-linking agent and the second cross-linking agent are the same or different. According to the preparation method, the problem of skin allergy caused by adding a chemical cross-linking agent is avoided by precisely regulating and controlling a dual cross-linking mechanism in molecules and between molecules, the stability and the supporting property of the silk fibroin are improved, and the degradation period of the silk fibroin is prolonged.

The invention discloses a near-infrared responsive injectable MXene / fibroin / carboxymethyl chitosan / oxidized hyaluronic acid nano composite hydrogel SCO (at) M, and relates to the technical field of biomedical materials. The method disclosed by the invention comprises the following steps: 1) preparing an MXene single-layer nanosheet by adopting LiF-HCl in-situ HF etching; (2) modifying the fibroin through glycidyl methacrylate to obtain SF-MA; 3) oxidizing hyaluronic acid with sodium periodate to obtain oxidized hyaluronic acid OHA; (4) uniformly dispersing MXene in the SF-MA solution, and sequentially adding carboxymethyl chitosan CMCS and OHA, so as to prepare the injectable nano composite soft hydrogel with the shear thinning characteristic; and 5) through ultraviolet irradiation crosslinking, rapid in-situ curing of the hydrogel is realized, and the mechanical strength of the hydrogel is improved. The hydrogel provided by the invention has excellent injectability and mechanical property, has good photo-thermal antibacterial property, oxidation resistance and cell compatibility, can be used as a carrier of cells, medicines and bioactive substances in the field of biological medicines, and has a wide application prospect.

The invention belongs to the technical field of cosmetic preparation, and particularly relates to a skin moisturizing and repairing composition based on dendrobium officinale and fibroin as well as a preparation method and application of the skin moisturizing and repairing composition. Essence extracted from dendrobium officinale tender shoots obtained through a plant stem cell culture technology and stepped molecular weight fibroin prepared through a composite enzymolysis technology serve as raw materials, the safe and efficient composition is developed, and the actual requirements for skin moisturizing and repairing are met.

The invention discloses a recombinant silk fibroin fusion protein as well as a preparation method and application thereof. The amino acid sequence of the recombinant silk fibroin is as shown in SEQ ID NO: 3. According to the invention, the silk fibroin is efficiently expressed through a genetic engineering method, and the recombinant silk fibroin or the recombinant silk fibroin which is high in expression and purity and can retain the efficacy and the activity of natural protein is finally obtained, so that the recombinant silk fibroin or the recombinant silk fibroin has relatively high industrial application potential.

The invention discloses uniform-particle-size silk fibroin microspheres and a preparation method and application thereof.The preparation method comprises the following steps that a regenerated silk fibroin solution is taken and subjected to trypsin digestion to obtain modified silk fibroin sediment, and the modified silk fibroin sediment is dissolved in an aqueous solution of a beta-folding inhibitor to obtain a silk fibroin solution serving as a dispersed phase; emulsifying through an external pressure type membrane emulsifier to obtain an emulsion containing the silk fibroin emulsion droplets; adding a cross-linking agent into the emulsion to carry out cross-linking reaction, and then adding a crystallization inducer to induce crystallization to obtain primary silk fibroin microspheres; and performing vacuum freeze drying to obtain the silk fibroin microspheres. Silk fibroin is subjected to enzyme digestion modification, silk fibroin fragments with high beta-folding content are reserved, and high structural stability is achieved; the crystallinity of the microspheres is improved by adopting a membrane emulsification mode and combining a multiple crystallization induction method, and the silk fibroin microspheres with uniform particle size and smooth surfaces are obtained.

The invention relates to the technical field of gas detection, in particular to response type aerogel and application thereof. The response type aerogel is prepared according to the following steps: mixing and dissolving a metal organic framework material and lead acetate to obtain an adsorption and response integrated probe; mixing silk fibroin, polyethylene glycol diacrylate and the adsorption-response integrated probe, and performing light curing reaction under the action of a photo-crosslinking agent to obtain the adsorption-response integrated probe. Wherein a double-grid aerogel substrate generated by reaction of silk fibroin and polyethylene glycol diacrylate can adsorb water molecules in environments with different humidity to wet the substrate, and a saturated microscopic water environment is provided for colorimetric reaction on the surface of a pore wall, so that interference of environmental humidity on response is effectively relieved. The quantitative method established by using the response type aerogel can be used for high-sensitivity quantitative detection of hydrogen sulfide in livestock and poultry farms.

The invention discloses a preparation method of antibacterial and hemostatic medical sponge, and relates to the technical field of preparation of medical sponge. The preparation method of the antibacterial hemostatic medical sponge comprises the following steps: cleaning silkworm cocoons after removing impurities, and immersing the cleaned silkworm cocoons in a neutral protease solution to complete degumming so as to obtain fibroin fibers; the preparation method comprises the following steps: dissolving chitosan in an acetic acid solution, dissolving polyvinyl alcohol in deionized water, adding sodium alginate, adding a silk fibroin solution to form a composite solution, dropwise adding an AgNO3 solution, introducing CO2, maintaining the pressure, quickly releasing the pressure, foaming, injecting into a mold, carrying out gradient freezing to form a multistage-aperture sponge matrix, immersing in a genipin ethanol solution, and transferring into a 5% CaC 12 solution for soaking. The silk fibroin provides a biocompatible substrate and promotes cell adhesion, the sponge is constructed into a hierarchical pore structure with a compact surface and a loose interior, and liquid absorption and blood coagulation factor enrichment are accelerated.

The invention relates to conductive silk fibroin loaded methacrylated gelatin hydrogel and a preparation method thereof, and belongs to the technical field of biomedical materials. The preparation method comprises the following steps: S1, adding silk fibroin fibers into a dopamine hydrochloride solution, stirring, standing, then adjusting the pH value to 8-9, and carrying out a heating reaction to obtain polydopamine grafted silk fibroin fibers; s2, sequentially dipping the polydopamine grafted silk fibroin fibers in an ammonium persulfate solution and a pyrrole solution to obtain conductive silk fibroin fibers; and S3, adding the conductive silk fibroin fibers into the methacrylated gelatin solution, carrying out ultraviolet light cross-linking curing, and then soaking the conductive silk fibroin fibers in a glycerol solution to obtain the conductive silk fibroin loaded methacrylated gelatin hydrogel. The method not only can ensure the structural integrity and conductive continuity of a conductive network in the hydrogel, but also breaks through the dependence of in-situ polymerization on the permeability of a matrix.

The invention relates to 3D printing silk fibroin-based conductive hydrogel as well as a preparation method and application thereof, and belongs to the technical field of hydrogel. The preparation method comprises the following steps: S1, adding silk fibroin, a graphene oxide dispersion liquid and glycidyl methacrylate into a lithium bromide solution for reaction, and performing dialysis, centrifugation and freeze drying to obtain modified silk fibroin; s2, dissolving the modified silk fibroin, polyethylene glycol diacrylate and a photoinitiator in water, and centrifuging and defoaming to obtain bio-ink; s3, preparing the bio-ink into the 3D printing silk fibroin-based conductive hydrogel through a DLP 3D printing technology. Methacrylic acid silk fibroin is used as a base material, polyethylene glycol diacrylate is used as a reinforcing material, and graphene oxide is introduced to construct the photocuring printing ink. In addition, the silk fibroin-based conductive hydrogel with excellent performance can be prepared by combining the structural design of multilevel porous and triple-period extremely small curved surfaces.

The invention relates to the technical field of woven fabrics, and particularly discloses a preparation process of a moisture-absorbing and sweat-releasing elastic woven fabric. The invention relates to a preparation process of a moisture-absorbing and sweat-releasing elastic woven fabric. The preparation process comprises the following steps: preparing yarns, weaving gray cloth, desizing, pre-preparing gray cloth, dyeing, cleaning, drying and absorbing, discharging and finishing, a padding finishing mode is adopted for absorption and discharge finishing, and a formula of finishing liquid comprises silk fibroin modified polyurethane, a polyether polyester block copolymer, amino silicon oil, organic acid and water. The moisture-absorbing and sweat-releasing elastic woven fabric manufactured by adopting the preparation process disclosed by the invention not only remarkably improves the air permeability and moisture absorption performance of the fabric and obviously improves the wearing comfort, but also further improves the washability and softness of the woven fabric.

The disclosure relates to peptides or protein fragments compositions, e.g., silk fibroin heavy chain, fibroin light chain, and / or fibrohexamerin (p25) derived peptides or protein fragments compositions.

The invention discloses silk fibroin-sodium alginate-chitosan bionic leather and a preparation method and application thereof.The preparation method comprises the following steps that a silk fibroin solution and a sodium alginate solution are mixed, after calcium salt is added, a chitosan solution continues to be added and mixed with waterborne polyurethane, and a mixed solution is obtained; and carrying out surface activation treatment on base cloth, coating the activated base cloth with the mixed solution, and carrying out drying curing and ultrasonic lamination to obtain the silk fibroin-sodium alginate-chitosan bionic leather. The silk fibroin, sodium alginate and chitosan bionic leather prepared by the invention not only has high strength and good ductility, but also has excellent tensile strength, folding resistance and wear resistance, and also has good biodegradability, so that the burden of waste post-treatment on the environment can be reduced, and the raw material cost and post-treatment cost of the bionic leather can be remarkably reduced; the method has both environmental benefits and outstanding economic benefits.

The invention provides a silk fibroin-based physical microgel material and a preparation method thereof, and belongs to the field of biomedical materials. According to the preparation method, an organic solvent is added into a silk fibroin solution, micron-sized gel particles with different sizes and different silk fibroin contents can be obtained by regulating and controlling the water-oil flow rate ratio and the silk fibroin concentration through the microfluidic technology, and the organic solvent and polysaccharide can be added into the silk fibroin solution at the same time; and the polysaccharide-silk fibroin mixed microgel is obtained through a microfluidic technology. The method is simple to operate and cost-saving, not only accelerates the sol-gel conversion speed of the silk fibroin, reduces the viscosity of the solution to enable the solution to pass through the micro-fluidic chip more easily, but also can promote the stability of the mixed solution of the silk fibroin and the polysaccharide, improves the mechanical property of the silk fibroin microgel and improves the stability of the micro-fluidic chip. The obtained silk fibroin-based microgel is uniform in size, regular in shape and high in porosity.

The invention relates to a composite bone repair material, a preparation method and application, the preparation method comprises the following steps: immersing silkworm cocoons in a soap salt solution for degumming, drying to prepare degummed silk, dissolving the degummed silk in a lithium bromide solution, dialyzing for desalting, and concentrating to obtain a regenerated silk protein solution; mixing the regenerated silk fibroin solution with a gelatin solution to obtain a gel solution; mechanically shaking the gel solution, so that the gel solution is uniformly mixed, uniformly distributed bubbles are formed in the gel solution, and a silk protein / gelatin gel block is obtained; soaking and cleaning the silk protein / gelatin gel block in an ammonium sulfate solution to obtain silk protein / gelatin cross-linked gel; and freeze-drying the fibroin / gelatin cross-linked gel to obtain the fibroin / gelatin composite material. The composite bone repair material has the advantages that the composite bone repair material has a bionic porous structure, has excellent mechanical properties and also has good biocompatibility and osteogenic activity. The material can be applied to filling and regeneration repair of skull defects of the orthopedics department, the oral cavity, the plastic surgery (maxillofacial) and the neurosurgery department, and has high industrial production and clinical application prospects.

The present invention relates to a silk fibroin-based underwater adhesive and a preparation method thereof. The method of the present invention uses natural silk as a raw material. After degummed silk is dissolved in a formic acid solution of inorganic salt, the silk fibroin / inorganic salt / formic acid solution is then coated on the part of the substrate that needs to be adhered, and immersed in water to obtain an adhesive that can adhere to the surfaces of various substrates underwater. The underwater adhesive prepared by the present invention is simpler in preparation, more convenient to use, lower in cost, green and environmentally friendly compared with other underwater adhesives, and has excellent adhesion ability. At the same time, the underwater adhesive prepared by the present invention has a wide range of raw material sources, can be rapidly degraded under certain conditions, has a certain promoting effect on resource and environmental protection, and has broad application prospects in the fields of packaging materials, medical materials, ship materials, etc.

The invention discloses a high-strength microneedle with directional holes for biological analysis sampling and a preparation method of the high-strength microneedle, and belongs to the technical field of microneedles, the method comprises the following steps: filling a silk fibroin solution into a microneedle mold to form a microneedle preform, the microneedle preform comprising a needle body part and a backing part; performing directional freezing on the microneedle preform to enable ice crystals to directionally grow in the direction from the backing part to the needle body part, and demolding to obtain a frozen blank; the frozen blank is placed in an organic solution of a cross-linking agent, soaked at the low temperature lower than 0 DEG C for ice template low-temperature solvent exchange-cross-linking treatment, taken out and cleaned, then soaked in a salt solution for salting-out treatment strengthening, taken out and cleaned and freeze-dried, and the high-strength microneedle with the directional holes is obtained. The microneedle prepared through the method has high strength and excellent puncture performance, and the skin interstitial fluid collection speed and efficiency are greatly improved.

The invention discloses an antibacterial dressing for promoting wound repair and regeneration and a preparation method thereof, and the preparation method comprises the following steps: adding hydrogen peroxide into a silk fibroin aqueous solution to prepare a silk fibroin aqueous solution premixed with hydrogen peroxide; horse radish peroxidase is added into the dopamine modified sodium alginate solution, and the dopamine modified sodium alginate solution premixed with horse radish peroxidase is prepared. Preparing a chondroitin sulfate composite solution; uniformly mixing the silk fibroin aqueous solution premixed with hydrogen peroxide, the dopamine modified sodium alginate solution premixed with horse radish peroxidase and the chondroitin sulfate composite solution. Hydrogel formed by cross-linking silk fibroin and chondroitin sulfate is used as a main body material of the dressing, and meanwhile, dopamine modified sodium alginate is introduced, so that the dressing has the photo-thermal antibacterial characteristic; magnesium oxide microspheres are introduced as a pore-foaming agent, micropores are formed in the dressing, and the liquid absorption property and the air permeability of the dressing are improved; the umbilical cord mesenchymal stem cell exosome is introduced, so that angiogenesis at the wound is promoted, and the wound healing promoting capability of the dressing is improved.

Provided are chemically modified silk fibroin and a use thereof in cell and organoid culture. The chemically modified silk fibroin is a product obtained by sequentially carboxylating silk fibroin and modifying same by an organic amine having a phenol group. Also provided is a hydrogel based on the chemically modified silk fibroin. A hydrogel system has a hierarchical structure and mechanical properties similar to those of an extracellular matrix, and exhibits characteristics such as definite composition, controllable physicochemical properties, low cytotoxicity, good biocompatibility, and biodegradability, can support the growth and differentiation of cells and organoids, and is suitable as a matrigel for cell and organoid culture.

The invention relates to methoxy polyethylene glycol epoxidation modified silk fibroin and a preparation method thereof, and belongs to the technical field of biological extraction. The method comprises the following steps: adding a low-concentration hydrogen bond inhibitor and a pH regulator into a regenerated silk fibroin solution, mixing, adding trypsin, incubating, inactivating, carrying out solid-liquid separation, and taking a solid phase to obtain a silk fibroin solid; then mixing and activating with an inorganic salt solution, performing epoxy co-incubation with methoxy polyethylene glycol after activation, and performing purification treatment to obtain a chemically grafted silk fibroin solution. The invention provides a low-concentration hydrogen bond inhibition-pH regulation combined enzymolysis technology, a dissolution modification technology of a precipitation layer after enzymolysis and a technical scheme of inorganic salt solution activation / methoxy polyethylene glycol epoxy synergistic chemical modification for the first time, so that the problems that the proteolysis effect is low and the enzymatic activity cannot be maintained for a long time are solved; and the precipitation layer is successfully prepared into a stable solution, so that the success rate of chemical modification of the silk fibroin is greatly improved, and subsequent further development and use are facilitated.

The invention discloses a silk fibroin solution injection capable of forming gel in situ and a preparation method and application thereof.The preparation method comprises the following steps that a regenerated silk fibroin solution with the molecular weight of 100 kDa-250 kDa and a hydrogen bond breaker are evenly mixed to obtain a mixed solution, the mixed solution is subjected to quick-freezing supercooling crystallization and vacuum drying to obtain silk fibroin powder, and the silk fibroin powder is dried to obtain the silk fibroin solution injection capable of forming gel in situ. And sterilizing and dissolving in normal saline to obtain the silk fibroin solution injection. A hydrogen bond breaker is introduced into a high-molecular-weight regenerated silk fibroin solution, a beta folding structure between silk fibroin molecules is inhibited, damage to the silk fibroin molecules in the freeze-drying process is reduced through a quick-freezing and supercooling crystallization method, and aggregation of the silk fibroin molecules is further reduced; after redissolving, an injection is formed and injected into local tissues, the micromolecular hydrogen bond breaker quickly diffuses, and the large molecular weight regenerated silk fibroin recovers hydrogen bond interaction and then forms hydrogel in situ through physical crosslinking, so that in-vivo in-situ gelling of the silk fibroin is realized.

The invention provides a dual-network hyaluronic acid-silk fibroin gel sponge and a preparation method thereof, and belongs to the technical field of gel sponge materials. The preparation method comprises the following steps: mixing sulfhydrylation modified hyaluronic acid and a silk fibroin solution, and sequentially performing freezing and soft freezing treatment to form the hyaluronic acid-silk fibroin gel sponge with a good shape. In the freezing induction process, hyaluronic acid generates chemical crosslinking through disulfide bonds, silk fibroin forms physical crosslinking through freezing concentration, and finally the stable gel-sponge coexistence body with the double-network structure is formed. The gel sponge has double advantages of gel and sponge. Meanwhile, due to freeze concentration treatment, the gel sponge has a macroporous structure, which is beneficial to potentially promoting adhesion, proliferation and migration of cells; the prepared gel sponge does not introduce additional chemical reagents, the biological safety of the gel sponge is guaranteed, and the gel sponge is suitable for the fields of cartilage / skin repair, tissue filling, wound dressing and the like.

The invention relates to a differentiated'inner core and outer sheath 'triboelectric stimulation bionic nerve graft and a preparation method thereof. The nerve graft is composed of an outer sheath layer and an inner core layer. Wherein an outer sheath layer is a calcium ion cross-linked waterborne polyurethane / sodium alginate double-network structure hollow conduit, and an inner core layer is oriented conductive polymer / silk fibroin nanofibers. The nerve graft provided by the invention is provided with the sheath part with an internal and external differential gradient hole structure and the bionic inner core similar to a natural nerve structure, when the nerve graft is implanted into a body, the inner core and the outer sheath show differential degradation speeds and hydrophilic-hydrophobic properties, and can generate electrical stimulation synchronously responding to physiological activities, so that regeneration after nerve injury is facilitated.

The application discloses sulfonated silk protein gel as an interface modification layer of a water-based zinc electrode and a preparation method thereof, and belongs to the technical field of negative electrode materials of zinc ion batteries. The sulfonated silk protein gel is prepared by using natural silk, raw materials are widely sourced, and the sulfonated silk protein gel is green and environment-friendly. The prepared sulfonated silk protein gel contains rich functional groups (‑OH, ‑NH‑, ‑C=O, ‑SO3H and the like), can cover the entire zinc electrode surface, forms a gel-like transition zone at the interface, and provides basic protection for the zinc electrode. In addition, the gel layer optimizes the electric field, improves the interface compatibility, can guarantee uniformity in the nucleation and growth process, plays an obvious role in controlling the dendrite growth, effectively prolongs the service life of the zinc ion battery, and improves the cycle stability of the zinc ion battery.