873 results about "Fibroin" patented technology

The invention discloses injectable sodium hyaluronate-silk fibroin composite hydrogel and a preparation method and application thereof.The injectable sodium hyaluronate-silk fibroin composite hydrogel is composed of sodium hyaluronate composite gel particles and a sodium hyaluronate solution, and the sodium hyaluronate composite gel particles comprise silk fibroin sponge microspheres and sodium hyaluronate gel particles; the silk fibroin sponge microspheres are loaded on the sodium hyaluronate gel particles, the silk fibroin sponge microspheres are physical crosslinking microspheres, the particle size D50 is 10-30 [mu] m, and the particle size D90 is within 100 [mu] m; the sodium hyaluronate gel particles are chemical cross-linked particles, the particle size D50 is 150-300 microns, and the particle size D90 is within 800 microns. After the sodium hyaluronate gel particles are in contact with tissues, sodium hyaluronate is preferentially degraded, and silk fibroin exposed in the degradation process has better degradation resistance and an effect of stimulating generation of collagen fibers, so that in-situ fixation of the composite gel particles is realized, and an instant and lasting filling effect is achieved; the material is a safe and effective medical beauty filling material.

According to the adjustable and controllable silk fibroin porous microspheres and the preparation method thereof, the microspheres with the size of 20 microns to 1 mm are prepared by adjusting technological parameters for preparing the microspheres. Pores are of a nano-submicron-micron multi-stage composite pore structure, and the porous microspheres have elasticity. The method provided by the invention shows wide application potential in the aspect of preparing silk fibroin microspheres with diversified sizes, and the microspheres have remarkable advantages in application scenes of medical injectable microspheres and medical embolism microspheres due to unique physical and chemical characteristics; the method has remarkable application value in multiple fields.

The present invention relates to the field of biomedicine technology, specifically a preparation method of a silk fibroin hyaluronic acid filler for injection. The present invention concentrates a silk fibroin solution to obtain a concentrated silk fibroin solution, and then dilutes it to obtain a diluted silk fibroin solution; the diluted silk fibroin solution is heated to form a silk fibroin gel, granulated to obtain silk fibroin gel particles; 1,4-butanediol diglycidyl ether and a sodium hydroxide solution are mixed uniformly, hyaluronic acid and 1 / 3 of the mass fraction of hydroxyapatite are added, after stirring uniformly, swelling, purification and granulation are carried out, and the remaining mass fraction of hydroxyapatite is added to obtain composite gel particles; the composite gel particles and the silk fibroin gel particles are mixed uniformly, added to the gel to mix, and a silk fibroin hyaluronic acid filler is obtained, which effectively prolongs its degradation time.

The invention discloses silk fibroin composite gel and a preparation method thereof, and relates to the technical field of tissue filling materials. The preparation method of the silk fibroin composite gel comprises the following steps: preparing silk fibroin and carrying out amination modification on the silk fibroin to obtain aminated silk fibroin; carrying out dynamic cross-linking on the aminated silk fibroin and the oxidized sodium alginate through a Schiff base bond by adopting an enzyme catalysis cross-linking process to obtain a dynamic cross-linked network solution; adding a temperature-sensitive component into the dynamic cross-linked network solution, and uniformly mixing to obtain a temperature-sensitive-dynamic dual-network pre-gel solution with temperature responsiveness; loading the active component into the temperature-sensitive-dynamic dual-network pregel solution to obtain a composite gel solution; incubating the composite gel solution to obtain primary gel; after disulfide bonds are introduced into the primary gel, cross-linking strengthening and sterilization are carried out, and the silk fibroin composite gel is obtained. Through temperature-sensitive-dynamic dual-network design, active components and disulfide bonds are introduced to strengthen crosslinking, and the problems that traditional injectable gel is irreversible, single in function, prone to collapse after being filled and the like are systematically solved.

The invention belongs to the field of biomedical materials, and particularly discloses a preparation method and application of a 3D printing porous composite scaffold. I-type collagen and silk fibroin composite gel is modified on a 3D printing three-element doped hydroxyapatite / polycaprolactone (PCL) composite scaffold with a polydopamine coating, the three-element doped hydroxyapatite is synthesized by using a chemical precipitation method, and Mg < 2 + >, Sr < 2 + > and SeO3 < 2-> ions are doped in crystal lattices. The 3D printing porous composite scaffold provided by the invention effectively simulates the structure and function of soft and hard combination of natural bones, is helpful for regulating and controlling the stem cell fate to improve the bone regeneration process, repairs bone defects while eliminating tumors, and can be applied to bone defect repair or osteosarcoma treatment.

The invention relates to a stable silk fibroin liquid condensation body, gel and a preparation method and application thereof, and belongs to the technical field of silk fibroin. The preparation method comprises the following steps: S1, dissolving silk fibroin in a lithium bromide solution, and then adding trypsin for incubation to obtain an enzyme-digested silk fibroin solution; and S2, performing low-temperature programmed centrifugal treatment including an induction stage, a nucleation stage and a liquid-liquid phase separation stage on the enzyme-digested silk fibroin solution, and cleaning to obtain the stable silk fibroin liquid-state aggregate, the method can reduce aggregate liquid drop aggregation and improve the particle size uniformity of the liquid drops.

The invention relates to a silk fibroin biological material with high beta-fold content and a preparation method thereof, and belongs to the technical field of biological extraction. The method comprises the following steps: preparing a regenerated silk fibroin solution; adding an enzyme catalytic promoter and an enzyme into the regenerated silk fibroin solution for incubation; the enzyme catalysis auxiliary agent is selected from one or more of a metal ion catalyst, a zwitterionic surfactant, a functionalized ionic liquid and a calcium phosphate nano-cluster; and centrifuging, cleaning and drying the obtained product to obtain the silk fibroin biological material with high beta-fold content. The silk fibroin material has higher beta-folding amount through primary structure modification, the space structure is more crystallized, and the amino acid sequence is more regular and ordered. The silk fibroin material provided by the invention has excellent mechanical properties, efficiency and yield, and is a biological protein material with great development potential.

The invention discloses a self-healing injectable photo-crosslinking dual-network hydrogel and a preparation method thereof.The preparation method comprises the steps that silk fibroin is subjected to methacrylation modification through a glycidyl methacrylate solution, then dextran of the silk fibroin is subjected to an oxidation reaction through a sodium periodate solution, oxidized dextran is generated, and the self-healing injectable photo-crosslinking dual-network hydrogel is obtained; the amino group in the modified silk fibroin and the aldehyde group of the oxidized dextran are subjected to a Schiff base cross-linking reaction to form the double-network hydrogel. The hydrogel with the double-network structure prepared by the invention is more compact in structure, so that the hydrogel has a series of excellent performances such as injectability, relatively good adhesion, good mechanical property, self-healing property and slow release property after drug loading while having safety.

The invention provides a silk fibroin hydrogel preparation method, which comprises: S1, dissolving degummed silk in a neutral salt solution to obtain a silk fibroin dissolving solution; s2, diluting the silk fibroin dissolving solution prepared in the step S1 with a diluent to obtain a silk fibroin dissolving diluent; s3, carrying out physical cross-linking treatment on the silk fibroin dissolving diluent prepared in the step S2 or regulating the pH value for physical cross-linking or adding a surfactant for physical cross-linking, and incubating to obtain the silk fibroin hydrogel. The silk fibroin hydrogel is simple in preparation process, high in gelling rate, low in loss and high in raw material utilization rate.

The present invention relates to the field of biomedical materials. Particularly provided is a DNA-silk fibroin composite hydrogel microsphere. The DNA-silk fibroin composite hydrogel microsphere is prepared from DNA and silk fibroin on the basis of the micro-fluidic technology by means of combination of self-assembly physical crosslinking and photo-crosslinking. The introduction of a silk fibroin network into the composite hydrogel microsphere can provide mechanical performance for a DNA hydrogel network and reduce the production cost. RGD can remarkably improve the cell loading capability of the microsphere. The hydrogel microsphere can simulate a microenvironment of cartilage tissue by means of three-dimensional loading of high-density cells, so as to enhance substance exchange and signaling between cells, and more effectively promote the proliferation and adhesion of bone marrow mesenchymal stem cells and regulating the chondrogenic differentiation, thus promoting cartilage regeneration and repair, and providing an available material for preparing medicaments or medical instruments for cartilage defect repair.

The invention discloses a silk protein-MXene composite film and a preparation method thereof. The silk protein-MXene composite film sequentially comprises a conductive surface layer, a porous response layer and a supporting bottom layer from top to bottom, the invention relates to the technical field of composite films. According to the fibroin-MXene composite film and the preparation method thereof, a three-layer gradient structure is formed by the conductive surface layer, the porous response layer and the supporting bottom layer, good conductivity and electromagnetic shielding effectiveness are ensured by utilizing oriented arrangement of MXene of the conductive surface layer, gallic acid and MXene form a hydrogen bond network, and nitrogen-doped carbon quantum dots coat the surface of MXene to form a physical barrier, so that the electromagnetic shielding effect is improved, and the electromagnetic shielding effect is improved. Through double oxidation resistance of gallic acid and nitrogen-doped carbon quantum dots, a sponge body structure formed by the porous response layer is matched to endow the composite film with high-sensitivity response to external stimulation, the supporting bottom layer is crosslinked by hexamethylene diisocyanate and enhanced by nanocellulose, the tensile strength is improved, and the tensile strength of the composite film is improved. And the contradiction between the mechanical property and the conductivity of the traditional composite film is effectively solved.

The invention provides double physical cross-linked silk fibroin composite gel as well as a preparation method and application thereof. A silk fibroin solution is cross-linked twice in a first cross-linking agent and a second cross-linking agent, the first cross-linking agent and the second cross-linking agent are hydroxyl cellulose or carboxyl cellulose or corresponding derivatives, the first cross-linking agent induces beta-folding in silk fibroin, the second cross-linking agent induces beta-folding among silk fibroin molecules, and the second cross-linking agent induces beta-folding among silk fibroin molecules. The first cross-linking agent and the second cross-linking agent are the same or different. According to the preparation method, the problem of skin allergy caused by adding a chemical cross-linking agent is avoided by precisely regulating and controlling a dual cross-linking mechanism in molecules and between molecules, the stability and the supporting property of the silk fibroin are improved, and the degradation period of the silk fibroin is prolonged.

The invention discloses a near-infrared responsive injectable MXene / fibroin / carboxymethyl chitosan / oxidized hyaluronic acid nano composite hydrogel SCO (at) M, and relates to the technical field of biomedical materials. The method disclosed by the invention comprises the following steps: 1) preparing an MXene single-layer nanosheet by adopting LiF-HCl in-situ HF etching; (2) modifying the fibroin through glycidyl methacrylate to obtain SF-MA; 3) oxidizing hyaluronic acid with sodium periodate to obtain oxidized hyaluronic acid OHA; (4) uniformly dispersing MXene in the SF-MA solution, and sequentially adding carboxymethyl chitosan CMCS and OHA, so as to prepare the injectable nano composite soft hydrogel with the shear thinning characteristic; and 5) through ultraviolet irradiation crosslinking, rapid in-situ curing of the hydrogel is realized, and the mechanical strength of the hydrogel is improved. The hydrogel provided by the invention has excellent injectability and mechanical property, has good photo-thermal antibacterial property, oxidation resistance and cell compatibility, can be used as a carrier of cells, medicines and bioactive substances in the field of biological medicines, and has a wide application prospect.

The invention belongs to the technical field of cosmetic preparation, and particularly relates to a skin moisturizing and repairing composition based on dendrobium officinale and fibroin as well as a preparation method and application of the skin moisturizing and repairing composition. Essence extracted from dendrobium officinale tender shoots obtained through a plant stem cell culture technology and stepped molecular weight fibroin prepared through a composite enzymolysis technology serve as raw materials, the safe and efficient composition is developed, and the actual requirements for skin moisturizing and repairing are met.

The invention discloses a recombinant silk fibroin fusion protein as well as a preparation method and application thereof. The amino acid sequence of the recombinant silk fibroin is as shown in SEQ ID NO: 3. According to the invention, the silk fibroin is efficiently expressed through a genetic engineering method, and the recombinant silk fibroin or the recombinant silk fibroin which is high in expression and purity and can retain the efficacy and the activity of natural protein is finally obtained, so that the recombinant silk fibroin or the recombinant silk fibroin has relatively high industrial application potential.

The invention discloses composite gel for osteochondral repair as well as a preparation method and application of the composite gel. Main raw materials of the composite gel are regenerated silk fibroin (RSF) and recombinant humanized III-type collagen (rhCol-III), and auxiliary raw materials of the composite gel are sodium hyaluronate (HA), fibrinogen and thrombin. The preparation method comprises the following steps: firstly, mixing an RSF solution with an rhCol-III solution, obtaining RSF-rhCol particles through a freeze drying technology and mechanical pulverization, dispersing the particles in an HA solution to obtain composite gel, mixing the gel with a thrombin solution, catalyzing fibrinogen to be split and converted into fibrin by thrombin, and performing freeze drying to obtain the rhCol-RhCol-RhCol-RhCol-RhCol-RhCol-RhCol. And crosslinking the fibrous protein monomer to obtain the injectable composite gel preparation capable of immediately gelling. The prepared RSF-rhCol composite gel can adapt to a defect area, has a certain adhesion effect, has excellent biocompatibility, promotes migration of bone marrow mesenchymal stem cells, induces cell differentiation and promotes cartilage tissue regeneration.

The invention discloses uniform-particle-size silk fibroin microspheres and a preparation method and application thereof.The preparation method comprises the following steps that a regenerated silk fibroin solution is taken and subjected to trypsin digestion to obtain modified silk fibroin sediment, and the modified silk fibroin sediment is dissolved in an aqueous solution of a beta-folding inhibitor to obtain a silk fibroin solution serving as a dispersed phase; emulsifying through an external pressure type membrane emulsifier to obtain an emulsion containing the silk fibroin emulsion droplets; adding a cross-linking agent into the emulsion to carry out cross-linking reaction, and then adding a crystallization inducer to induce crystallization to obtain primary silk fibroin microspheres; and performing vacuum freeze drying to obtain the silk fibroin microspheres. Silk fibroin is subjected to enzyme digestion modification, silk fibroin fragments with high beta-folding content are reserved, and high structural stability is achieved; the crystallinity of the microspheres is improved by adopting a membrane emulsification mode and combining a multiple crystallization induction method, and the silk fibroin microspheres with uniform particle size and smooth surfaces are obtained.

The invention relates to the technical field of gas detection, in particular to response type aerogel and application thereof. The response type aerogel is prepared according to the following steps: mixing and dissolving a metal organic framework material and lead acetate to obtain an adsorption and response integrated probe; mixing silk fibroin, polyethylene glycol diacrylate and the adsorption-response integrated probe, and performing light curing reaction under the action of a photo-crosslinking agent to obtain the adsorption-response integrated probe. Wherein a double-grid aerogel substrate generated by reaction of silk fibroin and polyethylene glycol diacrylate can adsorb water molecules in environments with different humidity to wet the substrate, and a saturated microscopic water environment is provided for colorimetric reaction on the surface of a pore wall, so that interference of environmental humidity on response is effectively relieved. The quantitative method established by using the response type aerogel can be used for high-sensitivity quantitative detection of hydrogen sulfide in livestock and poultry farms.

The invention discloses a preparation method of antibacterial and hemostatic medical sponge, and relates to the technical field of preparation of medical sponge. The preparation method of the antibacterial hemostatic medical sponge comprises the following steps: cleaning silkworm cocoons after removing impurities, and immersing the cleaned silkworm cocoons in a neutral protease solution to complete degumming so as to obtain fibroin fibers; the preparation method comprises the following steps: dissolving chitosan in an acetic acid solution, dissolving polyvinyl alcohol in deionized water, adding sodium alginate, adding a silk fibroin solution to form a composite solution, dropwise adding an AgNO3 solution, introducing CO2, maintaining the pressure, quickly releasing the pressure, foaming, injecting into a mold, carrying out gradient freezing to form a multistage-aperture sponge matrix, immersing in a genipin ethanol solution, and transferring into a 5% CaC 12 solution for soaking. The silk fibroin provides a biocompatible substrate and promotes cell adhesion, the sponge is constructed into a hierarchical pore structure with a compact surface and a loose interior, and liquid absorption and blood coagulation factor enrichment are accelerated.

The present invention relates to the technical field of layered materials, and discloses a moisture-absorbing quick-drying fabric and a preparation method thereof. The preparation method of the moisture-absorbing quick-drying fabric comprises the following steps: making modified cotton fiber into an inner layer fabric; applying a binary copolymer to one side of the inner layer fabric to obtain a hydrophobic inner layer fabric; forming a silk fibroin film on the side of the hydrophobic inner layer fabric that has not been hydrophobicized by electrospinning modified silk fibroin to obtain a modified inner layer fabric; making a surface layer fabric from special-shaped polyester fiber; the surface layer fabric is treated to obtain a modified surface layer fabric; laminating the modified surface layer fabric with one side of the silk fibroin film in the modified inner layer fabric, stacking and aligning, and compounding to obtain a moisture-absorbing quick-drying fabric. The moisture-absorbing quick-drying fabric of the present invention not only has excellent moisture-absorbing quick-drying performance and lasting antibacterial performance, but also has good anti-mite, mosquito-repellent performance and cool feeling.

The invention relates to conductive silk fibroin loaded methacrylated gelatin hydrogel and a preparation method thereof, and belongs to the technical field of biomedical materials. The preparation method comprises the following steps: S1, adding silk fibroin fibers into a dopamine hydrochloride solution, stirring, standing, then adjusting the pH value to 8-9, and carrying out a heating reaction to obtain polydopamine grafted silk fibroin fibers; s2, sequentially dipping the polydopamine grafted silk fibroin fibers in an ammonium persulfate solution and a pyrrole solution to obtain conductive silk fibroin fibers; and S3, adding the conductive silk fibroin fibers into the methacrylated gelatin solution, carrying out ultraviolet light cross-linking curing, and then soaking the conductive silk fibroin fibers in a glycerol solution to obtain the conductive silk fibroin loaded methacrylated gelatin hydrogel. The method not only can ensure the structural integrity and conductive continuity of a conductive network in the hydrogel, but also breaks through the dependence of in-situ polymerization on the permeability of a matrix.

The invention relates to 3D printing silk fibroin-based conductive hydrogel as well as a preparation method and application thereof, and belongs to the technical field of hydrogel. The preparation method comprises the following steps: S1, adding silk fibroin, a graphene oxide dispersion liquid and glycidyl methacrylate into a lithium bromide solution for reaction, and performing dialysis, centrifugation and freeze drying to obtain modified silk fibroin; s2, dissolving the modified silk fibroin, polyethylene glycol diacrylate and a photoinitiator in water, and centrifuging and defoaming to obtain bio-ink; s3, preparing the bio-ink into the 3D printing silk fibroin-based conductive hydrogel through a DLP 3D printing technology. Methacrylic acid silk fibroin is used as a base material, polyethylene glycol diacrylate is used as a reinforcing material, and graphene oxide is introduced to construct the photocuring printing ink. In addition, the silk fibroin-based conductive hydrogel with excellent performance can be prepared by combining the structural design of multilevel porous and triple-period extremely small curved surfaces.

The invention relates to the technical field of freeze-drying preparations, and discloses a preparation method of a controllable molecular weight silk fibroin composite freeze-drying filler, which comprises the following steps: preparing silk fibroin solutions with different molecular weights and a non-specific stabilizer into a composition liquid A, preparing microspheres, a microsphere dispersion liquid and purified water into a composition liquid B, and preparing the composition liquid B into the controllable molecular weight silk fibroin composite freeze-drying filler. Adding the composition liquid A into the composition liquid B, performing low-temperature swelling, performing uniform mixing, performing pre-cooling forming on the uniformly mixed freeze-drying composition liquid, and finally performing freeze-drying on the product by adopting a specific control program of a vacuum freeze-drying machine. According to the silk fibroin composite freeze-drying filling agent, the non-specific stabilizer, the microspheres and the microsphere dispersion carrier are adopted, so that the microspheres have good dispersity, and the silk fibroin composite freeze-drying filling agent is convenient to store, transport and use and has a relatively long period of validity.

The invention belongs to the technical field of biomedicine, and particularly relates to a high-strength regenerated silk fibroin material and a preparation method thereof.The preparation method specifically comprises the step that chemical crosslinking silk fibroin hydrogel is sequentially subjected to gradient alcohol treatment and vacuum drying treatment, and the high-strength regenerated silk fibroin material is obtained, the gradient alcohol treatment refers to staged treatment by adopting an ethanol aqueous solution, the concentration of the ethanol aqueous solution in each stage is fixed, and the concentrations of the ethanol aqueous solutions in different stages are increased along with the increase of stage serial numbers; the finally prepared high-strength regenerated silk fibroin material has a double-cross-linked network structure, and the double-cross-linked network structure is formed by intertwining a silk fibroin chemical cross-linked network and a beta folding structure physical cross-linked network. The preparation method disclosed by the invention is simple and easy to implement, and the compression mechanical property (the compression modulus is greater than or equal to 0.2 GPa) of the regenerated silk fibroin material is remarkably improved.

The invention relates to the technical field of woven fabrics, and particularly discloses a preparation process of a moisture-absorbing and sweat-releasing elastic woven fabric. The invention relates to a preparation process of a moisture-absorbing and sweat-releasing elastic woven fabric. The preparation process comprises the following steps: preparing yarns, weaving gray cloth, desizing, pre-preparing gray cloth, dyeing, cleaning, drying and absorbing, discharging and finishing, a padding finishing mode is adopted for absorption and discharge finishing, and a formula of finishing liquid comprises silk fibroin modified polyurethane, a polyether polyester block copolymer, amino silicon oil, organic acid and water. The moisture-absorbing and sweat-releasing elastic woven fabric manufactured by adopting the preparation process disclosed by the invention not only remarkably improves the air permeability and moisture absorption performance of the fabric and obviously improves the wearing comfort, but also further improves the washability and softness of the woven fabric.

The disclosure relates to peptides or protein fragments compositions, e.g., silk fibroin heavy chain, fibroin light chain, and / or fibrohexamerin (p25) derived peptides or protein fragments compositions.

The invention discloses silk fibroin-sodium alginate-chitosan bionic leather and a preparation method and application thereof.The preparation method comprises the following steps that a silk fibroin solution and a sodium alginate solution are mixed, after calcium salt is added, a chitosan solution continues to be added and mixed with waterborne polyurethane, and a mixed solution is obtained; and carrying out surface activation treatment on base cloth, coating the activated base cloth with the mixed solution, and carrying out drying curing and ultrasonic lamination to obtain the silk fibroin-sodium alginate-chitosan bionic leather. The silk fibroin, sodium alginate and chitosan bionic leather prepared by the invention not only has high strength and good ductility, but also has excellent tensile strength, folding resistance and wear resistance, and also has good biodegradability, so that the burden of waste post-treatment on the environment can be reduced, and the raw material cost and post-treatment cost of the bionic leather can be remarkably reduced; the method has both environmental benefits and outstanding economic benefits.

The invention provides a silk fibroin-based physical microgel material and a preparation method thereof, and belongs to the field of biomedical materials. According to the preparation method, an organic solvent is added into a silk fibroin solution, micron-sized gel particles with different sizes and different silk fibroin contents can be obtained by regulating and controlling the water-oil flow rate ratio and the silk fibroin concentration through the microfluidic technology, and the organic solvent and polysaccharide can be added into the silk fibroin solution at the same time; and the polysaccharide-silk fibroin mixed microgel is obtained through a microfluidic technology. The method is simple to operate and cost-saving, not only accelerates the sol-gel conversion speed of the silk fibroin, reduces the viscosity of the solution to enable the solution to pass through the micro-fluidic chip more easily, but also can promote the stability of the mixed solution of the silk fibroin and the polysaccharide, improves the mechanical property of the silk fibroin microgel and improves the stability of the micro-fluidic chip. The obtained silk fibroin-based microgel is uniform in size, regular in shape and high in porosity.

The invention relates to the technical field of railway transportation dust treatment, and particularly discloses an environment-friendly dust suppressant for coal transportation and a preparation method thereof.The environment-friendly dust suppressant is prepared from, by weight, 36-44 parts of functional components, 6-8 parts of modified sodium alginate, 0.02-0.06 part of initiator, 2-3 parts of phytic acid, 2.8-3.2 parts of genipin and 320-360 parts of deionized water; the functional component and the modified sodium alginate are added, and the functional component contains a xanthan gum structure, a Schiff base structure, a silk fibroin structure, an amino group and an ester group, so that the functional component can be entangled with a molecular chain structure of the modified sodium alginate to form an intertwined molecular interpenetrating network structure; meanwhile, the functional components have unsaturated double bonds capable of being chemically crosslinked with the modified sodium alginate, so that the wind erosion resistance, the mechanical property, the water resistance and the degradability of the environment-friendly dust suppressant are improved under the action of the structures.

The invention relates to a composite bone repair material, a preparation method and application, the preparation method comprises the following steps: immersing silkworm cocoons in a soap salt solution for degumming, drying to prepare degummed silk, dissolving the degummed silk in a lithium bromide solution, dialyzing for desalting, and concentrating to obtain a regenerated silk protein solution; mixing the regenerated silk fibroin solution with a gelatin solution to obtain a gel solution; mechanically shaking the gel solution, so that the gel solution is uniformly mixed, uniformly distributed bubbles are formed in the gel solution, and a silk protein / gelatin gel block is obtained; soaking and cleaning the silk protein / gelatin gel block in an ammonium sulfate solution to obtain silk protein / gelatin cross-linked gel; and freeze-drying the fibroin / gelatin cross-linked gel to obtain the fibroin / gelatin composite material. The composite bone repair material has the advantages that the composite bone repair material has a bionic porous structure, has excellent mechanical properties and also has good biocompatibility and osteogenic activity. The material can be applied to filling and regeneration repair of skull defects of the orthopedics department, the oral cavity, the plastic surgery (maxillofacial) and the neurosurgery department, and has high industrial production and clinical application prospects.