305 results about "Fibroin" patented technology

The invention discloses a high-strength microneedle with directional holes for biological analysis sampling and a preparation method of the high-strength microneedle, and belongs to the technical field of microneedles, the method comprises the following steps: filling a silk fibroin solution into a microneedle mold to form a microneedle preform, the microneedle preform comprising a needle body part and a backing part; performing directional freezing on the microneedle preform to enable ice crystals to directionally grow in the direction from the backing part to the needle body part, and demolding to obtain a frozen blank; the frozen blank is placed in an organic solution of a cross-linking agent, soaked at the low temperature lower than 0 DEG C for ice template low-temperature solvent exchange-cross-linking treatment, taken out and cleaned, then soaked in a salt solution for salting-out treatment strengthening, taken out and cleaned and freeze-dried, and the high-strength microneedle with the directional holes is obtained. The microneedle prepared through the method has high strength and excellent puncture performance, and the skin interstitial fluid collection speed and efficiency are greatly improved.

Provided are chemically modified silk fibroin and a use thereof in cell and organoid culture. The chemically modified silk fibroin is a product obtained by sequentially carboxylating silk fibroin and modifying same by an organic amine having a phenol group. Also provided is a hydrogel based on the chemically modified silk fibroin. A hydrogel system has a hierarchical structure and mechanical properties similar to those of an extracellular matrix, and exhibits characteristics such as definite composition, controllable physicochemical properties, low cytotoxicity, good biocompatibility, and biodegradability, can support the growth and differentiation of cells and organoids, and is suitable as a matrigel for cell and organoid culture.

The application provides a silk fibroin microsphere and a preparation method and application thereof, and belongs to the technical field of biopolymer microsphere preparation. The preparation method comprises the following steps: 1) adding an ionic dispersing agent into a silk fibroin solution, uniformly mixing, then adding a sphere forming agent into the solution, and obtaining a pretreated liquid after mixing; 2) performing low-temperature coagulation treatment on the pretreated liquid, and obtaining a silk fibroin microsphere solution after thawing; 3) diluting the silk fibroin microsphere solution, then performing wet heat crystallization treatment, and obtaining a high-crystallinity silk fibroin microsphere solution; and 4) performing spray drying on the high-crystallinity silk fibroin microsphere solution, and obtaining a solid silk fibroin microsphere. The silk fibroin microsphere obtained by the application has excellent biocompatibility, degradability and good mechanical properties. The silk fibroin microsphere prepared by the method of the application is regular spherical, has high crystallinity, slow degradation speed, is not easy to absorb moisture and adhere, and can be stored for a long time; and the preparation method is simple and has high yield.

The invention relates to the technical field of building materials, and discloses a mortar containing a nanometer material and a preparation method thereof, the mortar comprises 70-80 parts by weight of cement, 8-12 parts by weight of fly ash, 0.4-0.5 part by weight of a polycarboxylic acid water reducer, 0.4-0.6 part by weight of hydroxypropyl methyl cellulose, 1-2 parts by weight of the nanometer material, 2-3 parts by weight of modified silk fibroin and 40-50 parts by weight of water. Wherein the modified nano silicon dioxide is modified by gamma-aminopropyltriethoxysilane, the modified cubic nano calcium carbonate is subjected to compound modification by fumaric acid, sodium stearate and palmitic acid, and the modified silk fibroin is prepared by grafting modification by bis-aminopropyl terminated polysiloxane. According to the mortar, through optimization of component matching and modification treatment of the nano material and the silk fibroin, the mechanical property, durability and crack resistance of the mortar are remarkably improved, and the cost is controllable.

The invention provides a preparation method and application of a near-infrared light induction type silk fibroin nano preparation, and belongs to the technical field of medical preparations. And dropwise adding an ICG solution into the silk fibroin nano-particle solution, and then adding a ferric trichloride solution for reaction to obtain a precipitate, namely the near-infrared light induction type silk fibroin nano-preparation. The nano preparation prepared by the method disclosed by the invention can realize synergistic interaction of two treatment mechanisms, and the anti-tumor effect is remarkably improved.

The utility model discloses a mild child sanitary wet tissue, which belongs to the technical field of wet tissues and comprises an elastic soft non-woven fabric layer, a silk fibroin layer, a middle waterproof film and a sponge water absorption layer from top to bottom. A protruding part is arranged at one end of the middle waterproof film; a plurality of vertical through holes are evenly distributed in the sponge water absorption layer, and cleaning protrusions are arranged at the bottom of the sponge water absorption layer. When the cleaning pad is used, the side with the cleaning protrusions can be firstly used for cleaning the skin, dirt on the skin can be softened due to the fact that the sponge water absorption layer is high in water content, meanwhile, the starch coating is beneficial to improving the water absorption capacity of the sponge water absorption layer, and meanwhile a barrier is formed between the middle water insulation film and the sponge water absorption layer to avoid water loss; then the skin can be finely cleaned through the elastic soft non-woven fabric layer and the silk fibroin layer on the other side, the softness is high, the skin of children is prevented from being damaged, and the use is mild.

The invention discloses a water-soluble silk fibroin with a random crimp / Silk I structure as a main structure as well as a preparation method and application of the water-soluble silk fibroin. The silk fibroin product is characterized in that in a protein secondary structure, the total content of random curls and Silk I structures exceeds 85%, and the proportion of Silk I in the whole crystal structure is not lower than 75%; the product can be directly dissolved in water at room temperature to form a stable clarified solution with the concentration not lower than 5%, and is not gelatinized after being stored at 4 DEG C for 90 days. The preparation method comprises the following steps: degumming and cleaning the mulberry silk raw material; the method comprises the following steps: carrying out short-time (less than or equal to 20 minutes) high-temperature dissolution by adopting a multi-element solution dissolution system with a specific ratio, and then carrying out dialysis purification at a low temperature by taking the conductivity (lt, 10 [mu] S / cm) of a dialysis external liquid and the concentration (lt, 50 ppm) of calcium ions as double end-point standards. The directional regulation and control of the silk fibroin structure are realized through the accurate control of the whole process, and the obtained product has excellent water solubility, storage stability and biological safety, and can be widely applied to the fields of drug delivery, 3D biological printing, medical beauty filling, tissue engineering and the like.

The application discloses a preparation method of a silk fibroin film with adjustable surface roughness. The degummed silk fibroin is dissolved in a LiBr solution, and a regenerated silk fibroin solution is prepared through dialysis; the silk fibroin solution is poured to obtain a silk fibroin film, and the silk fibroin film is treated through salting-out, so that a silk fibroin film with different surface roughness is obtained. The application utilizes the interaction between water / inorganic salt and silk fibroin molecules to induce the conformation rearrangement of the silk fibroin molecules, and then promotes the crystallization and aggregation of the silk fibroin molecules, so that the surface roughness of the silk fibroin film is changed. Meanwhile, similar other physical properties are maintained.

The present disclosure relates to a method for manufacturing a molded article in which the molding ability of a protein is improved. Also provided is a composition for molding consisting of at least a mixture of a protein and a pyrolytic water discharge substance is compressed to produce a molded article.

This invention discloses a silk fibroin-based biomimetic artificial tympanic membrane with a gradient structure and its preparation method, comprising the following steps: encapsulating and dissolving an antibacterial drug in an organic solvent and then adding it dropwise to a silk fibroin solution; casting the film and inducing the formation of a β-sheet structure with methanol to obtain an outer dense layer; electrospinning the silk fibroin solution to obtain oriented silk fibroin fiber felt, and cross-linking it in an ethanol solution to obtain an intermediate fiber layer; mixing the silk fibroin solution with a pore-forming agent and casting it onto the intermediate fiber layer; covering the intermediate fiber layer with the outer dense layer; after pre-freezing and lyophilizing, immersing the membrane in deionized water to dissolve the pore-forming agent to form an inner porous layer; then placing it in a PBS buffer containing growth factors and shaking treatment; and finally drying to obtain the silk fibroin-based biomimetic artificial tympanic membrane with a gradient structure. This invention's three-layer biomimetic artificial tympanic membrane possesses antibacterial and regenerative functions, while also exhibiting excellent mechanical properties, meeting the requirements of a biomimetic tympanic membrane.

The invention relates to the technical field of biomedical materials, and provides a preparation method of a fibroin nanofiber reinforced silk protein gel material, the gel material and application, fibroin nanofibrils are prepared through a mechanical grinding method and introduced into composite hydrogel, the Young modulus reaches 4.68 MPa, and the fibroin nanofiber reinforced silk protein gel material is suitable for osteochondral repair and drug delivery.

The invention discloses a silk fibroin freeze-dried powder preparation process for improving specific surface area and porosity, and belongs to the technical field of biological materials, and the silk fibroin freeze-dried powder preparation process comprises the following steps: firstly, adding tert-butyl alcohol into a purified silk fibroin aqueous solution as a pore-foaming agent and a cosolvent to obtain a mixed solution; treating the mixed solution by adopting a multi-stage programmed freezing scheme which sequentially comprises three stages of slow precooling to construct a macroscopic ice crystal skeleton, constant-temperature annealing to realize crystal curing and rapid deep cooling to lock microcosmic ice crystals, so as to form a frozen sample with a graded ice crystal structure; and finally, removing water and tert-butyl alcohol through vacuum freeze drying to obtain a target product. The silk fibroin freeze-dried powder with a macroporous-microporous hierarchical porous structure can be controllably prepared through the synergistic effect of tert-butyl alcohol induction and programmed freezing, and compared with the prior art, the obtained product has ultrahigh porosity, remarkably increased specific surface area and uniform pore diameter.

The application relates to a high-elasticity chitosan / silk fibroin semi-interpenetrating network hydrogel and a photo-curing preparation method thereof. The high-elasticity chitosan / silk fibroin semi-interpenetrating network hydrogel is obtained by mixing a regenerated silk fibroin solution and a solution containing acylated chitosan, then performing ultrasonic treatment and photo-curing, the compressive stress is 1000-1200 kPa, the maximum strain is 50-60%, and the Young's modulus is 300-400 MPa. The high-elasticity chitosan / silk fibroin semi-interpenetrating network hydrogel provided by the application has good mechanical properties, elasticity and biocompatibility, and has a good application prospect in the field of biomedical materials.

The invention belongs to the technical field of biomedical materials, and particularly relates to an in-vivo degradable multifunctional hemostatic wound healing material as well as a preparation method and application thereof. 5-10 parts of chitosan with the degree of deacetylation greater than 90%, 0.8-1.2 parts of oxidizing bacteria nanocellulose and 0.8-1.2 parts of silk fibroin; dissolving chitosan powder in an acetic acid aqueous solution, and stirring until a clear faint yellow solution is formed, so as to prepare a chitosan solution; the preparation method comprises the following steps: dispersing frozen oxidizing bacteria nanocellulose and silk fibroin in deionized water, and stirring to form a uniform first suspension; and slowly dripping the chitosan solution into the first suspension while stirring at a high speed, centrifugally washing with deionized water, and finally freeze-drying the precipitate. The hemostatic material is excellent in hemostatic performance; a remarkable auxiliary antibacterial function is achieved, and the infection risk can be reduced; the biocompatibility and the degradability are excellent; the gel has double effects of promoting healing and resisting inflammation, and high-quality repair and regeneration of wounds are synergistically accelerated in multiple dimensions.

This invention belongs to the field of nanoenergy technology, and particularly relates to a method for preparing a multifunctional composite nanofiber membrane, a single-electrode triboelectric sensor, and its applications. The method includes the following steps: combining degummed and impurity-removed silk fibroin with a mixed solvent composed of anhydrous calcium chloride and formic acid, and stirring at room temperature to form a silk fibroin solution; adding TiO2 to the silk fibroin solution, and then adding phase change materials (PCMs) after stirring, followed by stirring and ultrasonic treatment to prepare a PCMs / TiO2 / silk fibroin spinning solution; and using solution jet spinning technology to convert the spinning solution into a silk fibroin-based composite nanofiber membrane. This composite nanofiber membrane has an adaptive temperature regulation function of daytime cooling and nighttime heat preservation, especially when applied to model cars, achieving daytime cooling up to 22°C. Simultaneously, the composite membrane also possesses excellent triboelectric properties and can be used to prepare a single-electrode triboelectric sensor with a maximum output power of approximately 272 μW / m. 2 It can realize sensing and monitoring functions in outdoor applications.

This invention discloses an enzyme-responsive silk fibroin nanovesicle, its preparation method, and its application, comprising the following steps: silk fibroin is mixed with a hydrophobic acylation reagent containing enzymatically cleavable chemical bonds and a catalyst for a grafting reaction to obtain amphiphilic modified silk fibroin; the amphiphilic modified silk fibroin is mixed with hair care active ingredients in an aqueous phase, and self-assembly is induced to form nanovesicles encapsulating the active ingredients through ultrasonic treatment or high-pressure homogenization. This invention uses silk fibroin as the main body to provide a biocompatible framework and self-assembly basis. Hydrophobic groups are introduced through chemical modification, and these hydrophobic groups are connected to the silk fibroin backbone through enzymatically cleavable chemical bonds. These chemical sites can be recognized and cleaved by sebaceous lipase secreted by the scalp sebaceous glands or other specific lipases highly expressed in the hair follicle microenvironment, achieving specific enzymatic release of the nanovesicles in the hair follicle microenvironment.

The invention provides a preparation method of a silk fibroin peptide nano-composite film with a heavy metal ion saturated adsorption visualization function. According to the method, an amino acid pairing (AAP) technology and a micro-fluidic reaction (MCR) technology are combined, the biocompatibility and the adsorption performance of silk fibroin peptide are utilized, and the silk fibroin peptide is compounded with a nano material, so that a novel nano composite film is prepared. The membrane not only can effectively adsorb heavy metal ions such as lead, cadmium, mercury and the like in a water body, but also has a visual function of adsorption saturation, so that a user can intuitively judge the use condition of an adsorption material through color change. The invention aims to prepare the silk fibroin peptide nano-composite membrane capable of efficiently adsorbing and visually displaying the saturated adsorption state of heavy metal ions, and the silk fibroin peptide nano-composite membrane is suitable for the fields of environmental governance, water quality monitoring and the like. The preparation method disclosed by the invention is simple, low in cost, easy for large-scale production, excellent in biodegradability and environment-friendly. The nano-composite membrane provided by the invention can realize rapid detection and treatment of water heavy metal pollution, and has important environmental and social benefits.

The invention relates to a method for obtaining silk fibroin powder through precipitation and application. Comprising the following steps: boiling silkworm cocoons in a first alkaline solution, and drying to obtain degummed silk; dissolving degummed silk in a phosphoric acid solution, and mixing to obtain a silk fibroin solution; diluting the silk fibroin solution with water, adding a second alkaline solution to adjust the pH value, separating and collecting silk fibroin precipitate; washing the silk fibroin precipitate with water to be neutral, and performing precooling and freeze drying to obtain silk fibroin powder. According to the preparation method, the silk fibroin is dissolved by phosphoric acid with the temperature of 10 DEG C below zero to 10 DEG C, the silk fibroin solution is subjected to deprotonation treatment and then freeze-dried, the silk fibroin powder of the random curled / spiral structure can be rapidly obtained, long-time dialysis is avoided, and the preparation time of the silk fibroin powder is shortened. The obtained regenerated silk fibroin powder can be used for preparing a high-strength silk fibroin material through hot press molding, and the bending strength reaches up to 179 MPa.

This invention discloses an artificial corneal endothelial graft based on natural degradable proteins, its preparation method, and its application, relating to the field of medical implant materials technology. Currently, artificial corneal endothelial grafts for corneal endothelial decompensation are all non-degradable synthetic polymer materials. This invention uses a natural degradable material as the substrate to prepare the graft. A material preparation stock solution is obtained by mixing a silk fibroin solution extracted from a high-salt solution with a certain proportion of glycerol. The mixed solution is then spin-coated onto a mold using a gradient method, and dried to obtain a silk fibroin film. The obtained silk fibroin film is then circumcised and placed in a mold for hot-press steam fumigation. The resulting silk fibroin film can serve as an artificial corneal endothelial graft, providing a donor source for artificial corneas and possessing potential application value in corneal transplantation.

The invention relates to the technical field of child skin care products, in particular to a child skin soothing and repairing cream containing egg yolk oil extract and a preparation method of the child skin soothing and repairing cream. The prepared children skin soothing and repairing cream is long in acting time and high in repairing efficiency; wherein the egg yolk oil hydrogel beads are easy to break at the damaged skin, and the egg yolk oil emulsion is released, so that the action time can be prolonged, the egg yolk oil emulsion is released at the damaged skin in a targeted manner, and the utilization rate and the repair efficiency are effectively improved; the silk fibroin microspheres reduce active oxygen and have good ultraviolet resistance; through the synergistic effect of the components in the plant extract, various problems of fragile skin barrier, dryness, water shortage, redness, inflammation and the like of children can be effectively solved; the auxiliary materials can promote mutual fusion of the components while playing a role in moistening and moisturizing, and compared with the prior art, the composition has a wide application prospect.

The application provides a silk fibroin nanofiber composite film and a preparation method and application thereof. The preparation method comprises the following steps: preparing a self-supporting film with an ordered and continuous fiber network framework from a silk fibroin nanofiber dispersion liquid, wherein the fiber network framework serves as a reinforcing phase; contacting the self-supporting film with a solution containing a matrix phase and / or a functional phase, so that the matrix phase and / or the functional phase is filled into the fiber network framework of the self-supporting film to obtain a composite precursor film; and extruding the composite precursor film, or extruding and cross-linking the composite precursor film to obtain the silk fibroin nanofiber composite film. The method of the application adopts a time sequence separation strategy of 'first making a framework and then filling', and the self-assembly ordered structure of the silk fibroin nanofiber is completely reserved. The reinforcing phase, the matrix phase and the functional phase in the obtained composite film synergistically act, and the composite film has high mechanical strength, low swelling rate and functional integrability, and can be applied to the fields of implantable medical devices or biosensors.

The present invention relates to the techniques of hair styling. In particular, the invention provides a hair treatment combining a silk fibroin hydrolysate and high molecular weight 5 polyethylene glycols (PEGs) in a sequential process.

The application discloses a kind of photo-cured silk fibroin double-layer hydrogel and preparation method thereof, belong to medical dressing technical field.The double-layer hydrogel includes upper silk fibroin hydrogel, lower hyaluronic acid hydrogel.The application is covalently bridged by interface transition layer mussel myopic double mechanism through ultraviolet light excitation, realizes upper and lower covalent crosslinking, improves interlayer bonding strength.Two-step photo-curing process is used, and the whole process time is less than 70 seconds, and the preparation is simple, fast and easy to operate.The double-layer hydrogel can simulate the "epidermis-dermis" double-layer structure of skin, has good biocompatibility and moisturizing property, and effectively promotes wound repair.

The application discloses a kind of biomimetic silk fibroin cartilage scaffolds for cranium defect repair, belong to biomedical engineering technical field.To solve the problem of single structure, low bone formation efficiency and mismatching of mechanical properties and degradation rate of existing cranium repair scaffold, the application provides a kind of scaffold, which includes in order from outside to inside: biomimetic periosteum layer, bone conduction and vascularization layer and cartilage induction layer.Biomimetic periosteum layer is dense nanofiber membrane structure, and plays the role of physical barrier and osteoinduction;Bone conduction and vascularization layer is gradient porous structure with gradually decreasing aperture from outside to inside, aiming to guide the ordered growth of blood vessels and cells, and promote rapid vascularization;Cartilage induction layer is sponge-like microporous structure, used for forming stable cartilage template and starting endochondral ossification.The application simulates the natural bone healing process through structural biomimicry and functional zoning, synergistically promotes vascularization and osteogenesis, and realizes efficient biological repair of cranium defect.

The invention relates to the technical field of polymer composite materials, in particular to a highly wear-resistant and scratch-resistant PET film for an automobile and a preparation method of the highly wear-resistant and scratch-resistant PET film. The highly wear-resistant and scratch-resistant PET film is prepared from the following raw materials in parts by weight: 100 parts of PET matrix resin, 5-15 parts of an additive A, 2-8 parts of an additive B, 0.3-1 part of an antioxidant 1010, 0.5-1 part of an ultraviolet light absorber and 0.5-2 parts of calcium stearate. The additive A forms a cross-linked network with silk fibroin and chitosan through a treating fluid, a silane coupling agent is introduced to enhance interface bonding, the filling density and surface hardness of the PET film are effectively enhanced, the additive B takes sodium alginate as a framework, and a silicon dioxide composite structure formed by hydrolyzing a pretreated cardanol derivative and tetraethoxysilane is combined, so that the filling density and surface hardness of the PET film are improved. Through the synergistic effect of microcosmic toughening and inorganic particles, the wear resistance and scratch resistance of the PET film are improved, and the prepared PET film has the comprehensive properties of wear resistance and scratch resistance.

The invention discloses a degradable ventilation drainage tube material and a preparation method thereof. The degradable ventilation drainage tube material is a silk fibroin / polylactic acid composite material. The preparation method comprises the following steps: preparing granular silk fibroin by adopting an emulsion-freezing phase separation method; preparing a composite spinning solution of granular silk fibroin and polylactic acid; spinning the composite spinning solution to obtain a granular silk fibroin / polylactic acid composite fiber membrane; and carrying out post-treatment and sterilization on the granular silk fibroin / polylactic acid composite fiber membrane. According to the composite material disclosed by the invention, through a bionic fiber network structure, the mechanical properties of silk fibroin and polylactic acid are integrated, and the supporting property of silica gel is surpassed; the catheter has flexibility and elastic modulus matched with the eustachian tube tissue, the smoothness of the catheter can be guaranteed, tissue stimulation can be reduced, and the comfort level of a patient is improved.

The invention discloses a detachable silk fiber with a slow release function and a preparation method thereof, and the preparation method comprises the following steps: mixing polyvinyl alcohol and deionized water to obtain a solution A; mixing the solution A with essential oil to obtain a solution B; mixing fibroin powder with deionized water to obtain a solution C; mixing the solution C and the solution B, and adding absolute ethyl alcohol to obtain a solution D; freezing the solution D, unfreezing, and centrifuging to obtain silk fibroin essential oil microcapsule powder; mixing the silk fibroin essential oil microcapsule powder with deionized water to obtain spraying liquid; and spraying the spraying liquid on the surface of the silk fiber to obtain the detachable silk fiber with the slow-release function. The prepared detachable silk fiber with the slow-release function has a long-acting slow-release function, and when the slow-release function disappears or is not needed, the protective film layer on the surface of the silk fiber can be detached.

The invention relates to the field of medical beauty industry, and particularly discloses intelligent response type hydroxyapatite medical beauty gel as well as a preparation method and application thereof. The intelligent response type hydroxyapatite medical beauty gel is prepared from the following raw material components: 90 to 95 parts of hydroxyapatite, 35 to 40 parts of regenerated silk fibroin composite magnetic microspheres, 50 to 55 parts of hyaluronic acid, 20 to 25 parts of recombinant III type humanized collagen and 15 to 20 parts of basic fibroblast growth factor sustained release microspheres. The preparation method comprises the following steps: uniformly dispersing all the components in hyaluronic acid, and stirring to obtain the medical beauty gel. The intelligent response type hydroxyapatite medical beauty gel belongs to an important gel material in orthopedic repair in the medical beauty industry, can well repair a bone defect part, and has excellent osteogenesis performance, biocompatibility and biological safety in vivo; and the product has the great advantages of high safety and good beautifying effect in medical beautifying bone shaping and bone repair.

The present application belongs to the technical field of packaging materials, and particularly relates to a peritoneal dialysis solution bag. The peritoneal dialysis solution bag comprises, from inside to outside, an inner layer, a heat preservation layer and an outer layer. The heat preservation layer is prepared from hollow fibers. The hollow fibers are obtained by spinning raw materials including chitosan, silk fibroin and hydroxyapatite. The prepared peritoneal dialysis solution bag has good heat preservation performance and barrier performance.

Described herein is a method of purifying a silk fibroin preparation including preparing an aqueous silk fibroin solution having a concentration of greater than or equal to 5% w / v silk fibroin from the silk fibroin preparation, wherein the silk fibroin preparation includes a chaotropic salt; and exchanging the chaotropic salt from the silk fibroin solution at a pH below the isoelectric point of the silk fibroin, wherein the pH is between 2 and 5, replacing the chaotropic salt with a buffer comprising 10 to 300 mM of a second salt, or a combination thereof, to prepare the purified silk fibroin.