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30 results about "Antheraea pernyi" patented technology
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Antheraea pernyi, the Chinese (oak) tussar moth, Chinese tasar moth or temperate tussar moth, is a large moth in the family Saturniidae. The species was first described by Félix Édouard Guérin-Méneville in 1855. Antheraea roylei is an extremely close relative, and the present species might actually have evolved from ancestral A. roylei by chromosome rearrangement.
Antheraea pernyi silk fibrion biology medicine material and the preparation method
InactiveCN101036802AGood biocompatibilityIdeal induced biomedical materialsSurgeryProsthesisTissue repairReticular formation
The invention discloses bio-medical engineering material and preparation method thereof, particularly relates to 3D tissue repair material suitable for adhesively growing of cell and preparation method thereof, belonging to bio-medical material technique domain. The natural tussah silk is used as raw material. After antheraea pernyi silk fibroin dissolves, 3D non-woven web structure composed of antheraea pernyi silk fibroin fibre is obtained by electrostatic spinning, wherein the fibre diameter is 50nm-20 mu m, the hole between the fibres is 1-500 mu m, the thickness of non-woven web is 50nm-20mm, and molecular weight of the antheraea pernyi silk fibroin is 10X10<4>-20X10<5>D. The bio-medical engineering material has features of non-poison, harmless, good biocampatibility, good adhesive growth ability of cell, etc. Because of the wide materials supply and low cost, the said material will replace some high-cost bio-medical material such as collagen, and will be used for repair material such as artificial skin, tendon, cartilage, duramater and so on, particularly for histiocyte induction material.
Owner:SUZHOU UNIV
Artificial multiplication method for tetrastichus septentrionalis
The invention discloses an artificial multiplication method for tetrastichus septentrionalis. The method includes the following steps of (1) selection of a substitute host; (2), field collection and selection of micromelalopha troglodyta pupa; (3), cultivation of bee species; (4) extraction of information matter; (5), processing of antheraea pernyi pupa; (6), bee receiving; (7), cultivation and development. According to the artificial multiplication method, pupa which is not subjected to parasitism is soaked with ethyl alcohol, pupa surface extract is obtained, then the pupa surface extract is sprayed on the antheraea pernyi pupa in an artificial spraying mode, the tetrastichus septentrionalis is induced to be subjected to parasitism, and the parasitism rate is raised. The tetrastichus septentrionalis is subjected to indoor large-scale multiplication, and a practical technical means is provided for preventing and controlling hyphantria cunea, micromelalopha troglodyta and other lepidoptera pests for forest creatures.
Owner:LUOHE CITY FOREST PEST CONTROL & QUARANTINE STATION
Antheraea pernyi silk fibrion biomedicinal material and the preparation method
InactiveCN100551449CGood biocompatibilityIdeal induced biomedical materialsSurgeryProsthesisCell adhesionReticular formation
The invention discloses a biomedical engineering material and a preparation method thereof, in particular to a three-dimensional tissue repair material and a preparation method thereof, which belong to the technical field of biomedical materials. It uses natural tussah silk as raw material. After the tussah silk fibroin is dissolved, the electrostatic spinning method is used to obtain a three-dimensional non-woven network structure composed of tussah silk fibroin fibers. The fiber diameter is 50nm-20μm, and the pores between the fibers are 1-500μm. , the thickness of non-woven net is 50nm~20mm; the molecular weight of tussah silk fibroin is 10×104~20×105D. This biomedical engineering material has the characteristics of non-toxic and harmless, good biocompatibility, and excellent cell adhesion and growth performance. It is rich in raw materials and low in cost. It is expected to replace high-cost biomedical materials such as collagen for artificial skin, Repair materials such as tendon, cartilage, and dura mater, especially tissue cell induction materials.
Owner:SUZHOU UNIV
Method for preparing Antheraea Pernyi powder and Antheraea Pernyi powder thereof
InactiveCN102318819AKeep active ingredientsSimple processFood preparationHard CapsuleActive component
The invention relates to a method for preparing Antheraea Pernyi powder and Antheraea Pernyi powder thereof. The method comprises the following steps: heating cocoons of Antheraea Pernyi to obtain moths, spawning, cleaning and disinfecting the Antheraea Pernyi, refrigerating and freezing, heating at the temperature of -45 DEG C under 0.01MPa-0.09MPa for 2-5 DEG C / hour to the temperature of 30 DEG C-40 DEG C, insulating for 2-3 hours and then refrigerating, vacuum drying, crushing in a low temperature pulverizer at the low temperature of 0 DEG C- -35 DEG C to 100 meshes and sieving. The main quality index of the Antheraea Pernyi powder are: nucleic acid (mg / g) >=30.0, free amino acid (mg / g) >=12.0, VE (ug / g) >=2.0, bacterium colony total amount (CFU / g) <=3000. The invention is characterized in that the low temperature pulverization is employed for directly processing the fresh oophyte which greatly reserves the active component of Antheraea Pernyi and is suitable for the requirements of high-grade health product and medicine production. The particle degree of the ultrafine powder at low temperature reaches more than 100 meshes, so that the biological utilization degree can be enhanced and the medicinal dosage forms such as hard capsule can be conveniently processed. The method has the advantages of simple technology and convenient operation, the product has the advantages of easy preservation and convenient application which solves the problems of transportation and fresh-preservation, the product also has the merits of moderate cost, high benefit and wide market prospect.
Owner:辽宁柞蚕丝绸科学研究院有限责任公司
Antheraea pernyi artificial feed
ActiveCN104920818AImprove palatabilityNutritional diversityFood processingAnimal feeding stuffAcornVitamin C
The present invention discloses an antheraea pernyi artificial feed which includes the following components: acorn kernel meal, oak leaf powder, multi-vitamin glucose powder, vitamin C and nipagin ester. The mass ratio of each component is as follows: acorn kernel meal 75-85 parts, oak leaf powder 10-20 parts, multi-vitamin glucose powder 0.5-1.0 part, vitamin C 0.05-0.1 part and nipagin ester 0.1-0.2 part. The artificial feed uses the oak tree fruit acorn kernels as main raw materials, and has strong palatability, full nutrition and high feeding rate, can enable young silkworms to develop well, has simple formula, simple manufacturing processes, and low costs, and is easy to promote, etc.
Owner:JILIN SERICULTURE SCI RES INST
Artificial feed formula for cantheconidae furcellata
The invention provides an artificial feed formula for cantheconidae furcellata. The artificial feed formula for cantheconidae furcellata comprises 10-30 parts by weight of maggot meal, 10-20 parts byweight of bovine livers, 5-15 parts by weight of yolk, 5-17.5 parts by volume of antheraea pernyi haemolymph, and 3.5-8 parts by weight of honey. The artificial feed formula can be used for feeding cantheconidae furcellata in replacement of tenebrio molitor; moreover, the artificial feed formula is capable of ensuring normal growth and development of cantheconidae furcellata, as well as improvingnymph survival rate and offspring hatch rate of the cantheconidae furcellata.
Owner:SOUTH CHINA AGRI UNIV
Antheraea pernyi ovary cell culture medium and application thereof
ActiveCN102618487AComprehensive nutrition for growthEasy to operateArtificial cell constructsVertebrate cellsBiotechnologyPenicillin
The present invention discloses composing components of an antheraea pernyi ovary cell culture medium, a preparation method of the antheraea pernyi ovary cell culture medium, and an application of the antheraea pernyi ovary cell culture medium in antheraea pernyi ovary cell culture. According to the present invention, a prepared amino acid and sugar mixing storage solution (MLM-AAS), an inorganicsalt storage solution (MLM-Salt), a vitamin storage solution (MLM-Vit), bovine serum albumin, fetuin and the like are prepared according to a certain ratio, and the pH value is adjusted to 6.2-6.4 toobtain a MLM-45 culture medium, wherein the prepared MLM-45 culture medium is added with fetal bovine serum and a penicillin and streptomycin mixing solution according to a certain volume ratio before the prepared MLM-45 culture medium is used, the volume ratio of the fetal bovine serum is 20%, and the volume ratio of the penicillin and streptomycin mixing solution is 1%. With a plurality of experiments, the following in vitro cell growth conditions are determined that: the culture temperature is 26-28 DEG C, the pH value is 6.2-6.4, and the osmotic pressure is 315-350 mOsm / kg. With the present invention, technical services are provided for researches in antheraea pernyi biology, antheraea pernyi pathogenic microorganism and related fields.
Owner:辽宁省农业科学院大连生物技术研究所
Construction method and application of antheraea pernyi multinucleocapsid nucleopolyhedrovirus shuttle vector
ActiveCN110592139AInfectiousImprove recombination efficiencyFermentationDsDNA virusesEscherichia coliShuttle vector
The invention discloses a construction method and application of an antheraea pernyi multinucleocapsid nucleopolyhedrovirus shuttle vector, and belongs to the field of a biological technology. A bacterium artificial chromosome cloning vector is used for constructing a transfer vector comprising a DNA fragment containing a lacZ alpha report gene and a bacterial transposon Tn7 transposition target point sequence, a DNA fragment containing an AnpeNPV polyhedrin gene promoter sequence and an upstream gene partial sequence, and a DNA fragment containing a polyhedrin gene 3' terminal partial codingregion sequence and a downstream gene partial sequence, and linearized transfer vector plasmid DNA and linearized AnpeNPV genomic DNA are subjected to cotransfection with Tn-High Five cells, so that recombinant AnpeNPV is obtained. The recombinat AnpeNPV genomic DNA is transformed to escherichia coli, so that an AnpeNPV shuttle vector AnpeNPV-bacmid is obtained. The shuttle vector can be reproduced in the manner of plasmid DNA in the escherichia coli, can also be reproduced in the virus DNA manner in Tn-High Five insect cells and antheraea pernyi, and has infectivity.
Owner:LIAONING OCEAN & FISHERIES SCI RES INST
Extraction method of wild antheraea pernyi silk fibroin
InactiveCN107216376AEasy extractionEasily biodegradablePeptide preparation methodsAnimals/human peptidesUltrafiltrationFreeze-drying
The invention relates to the field of silk fibroin extraction, and discloses an extraction method of wild antheraea pernyi silk fibroin. The extraction method comprises the following steps: degumming and drying tussah silks; extracting the wild antheraea pernyi silk fibroin with a mixed solution system of FeCl2 and tetrasodium iminodisuccinate, and adjusting pH under ultrasonic assistance in an extracting process in order to increase the extraction rate; performing ultrafiltration on a solution undergoing ultrasonic treatment, performing freeze drying on the remaining solution after ultrafiltration, and grinding the obtained wild antheraea pernyi silk fibroin into powder for later use. The method provided by the invention is easy to operate, and has low environmental harm and high protein extraction rate; the obtained silk fibroin has higher purity.
Owner:ZHEJIANG SCI-TECH UNIV
Antheraea pernyi food and preparation method thereof
ActiveCN105661518ASimple processEasy to operate and implement transformationFood preservationBiotechnologyDesiccant
A preparation method of antheraea pernyi food is characterized by comprising steps as follows: material selection: fresh antheraea pernyi or fresh and timely frozen antheraea pernyi is selected; removal of scaly hair: a part of scaly hair on the body surface of antheraea pernyi is sieved out with a sieve with the mesh diameter of 0.5-1 cm, and the antheraea pernyi is left for standby; drying: the selected antheraea pernyi is put in a microwave drying machine, and the antheraea pernyi is completely dried through microwave drying, and the obtained antheraea pernyi food with an original flavor can be obtained; or the selected fresh antheraea pernyi can be subjected to microwave drying and cooked, the cooked antheraea pernyi is immersed in a spiced, spicy, sweet and spicy or cumin seasoning juice formed by mixing seasonings with a proper amount of water, soaked, pickled and subjected to microwave drying, and the flavored antheraea pernyi food is obtained; the antheraea pernyi food is cooled to the room temperature and subjected to vacuum packaging, and an antioxidant desiccant is put into each package or nitrogen-filling packaging is performed. The antheraea pernyi does not shrivel after being dried, the appearance is plump, and the antheraea pernyi food is easy to eat and tastes crisp. The antheraea pernyi food is cooled to the room temperature and then subjected to vacuum packaging or nitrogen-filling packaging, can be eaten after the bag is opened, is convenient to store and transport and is nutritional food for people at home, in travel or in leisure time.
Owner:JILIN SERICULTURE SCI RES INST
Coating material, hermetia illucens larva living thing coating bait, and preparation method and application
The invention provides a coating material, a hermetia illucens larva living thing coating bait, and a preparation method and application, and belongs to the techncial field of application of industrial aquaculture baits. The coating material comprises the following preparation raw materials of defatted soy flour, antheraea pernyi powder, soybean oil, corn starch, multivitamin and minerals tablets,calcium, phosphorus, magnesium, zinc and ferrum rapidly enriching tables for livestock and xanthan gum. The coating material is reasonable in matching of nutrient components, and the hermetia illucens larva living thing coating bait prepared from the coating material can meet requirements of Chinese forest frogs for growth, development, oil generation and propagation. The hermetia illucens larvaliving thing coating bait solves the problems that at current, the forest frog living insect bait is single in kinds, and unbalanced in nutrients, the cost of the baits is greatly reduced, and the content of nutrient components required by the forest frogs can be manually controlled and balanced, so that the oil quality of forest frogs which are artificial bred and the oil quality of wild forest frogs are not obvious in difference, the oil generating period is shortened by 30%, and the hermetia illucens larva living thing coating bait has important significance in realization of industrial large-scale cultivation techniques of the Chinese forest frogs.
Owner:JILIN SERICULTURE SCI RES INST
Artificial diet for univoltine Antheraea pernyi
InactiveCN110301535AReduce manufacturing costGreat tasteAnimal feeding stuffAccessory food factorsMongolian oakFood additive
The invention discloses an artificial diet for univoltine Antheraea pernyi and relates to the technical field of farming. The artificial diet for univoltine Antheraea pernyi is made from 38-42% of Quercus mongolica leaf powder, 16-22% of fresh soybean powder, 18-20% of corn flour, 5-15% of agar powder, and 3-7% of cane sugar. Auxiliaries include, by weight, 2-3% of citric acid, 0.4-0.6% of tannicacid, 0.35-0.45% of sorbic acid, 0.6-1.4% of VC, 0.3-0.7% of VB, 0.05-0.15% of florfenicol, 1-2% of inorganic salts, and 0.3-0.8% of choline chloride. The Quercus mongolica leaf powder, fresh soybeanpowder, corn flour and agar powder are used as main materials; Quercus mongolica leaf is mainly mixed with the fresh soybean powder, corn flour, agar powder and other low-price materials, so that theproduction cost of the artificial diet is reduced, and nutrients can be balanced; cane sugar, citric acid, tannic acid, sorbic acid, VC, VB, florfenicol, the inorganic salts, choline chloride and other food additives are added, organic acids, bases and inorganic salts required by the growth of young silkworms are supplemented, and therefore, antibacterial capacity of the young silkworms is improved, good taste is given to the artificial diet, and diet consumption rate of silkworms is increased.
Owner:河南省蚕业科学研究院
Indoor all-age antheraea pernyi breeding device and method
The invention relates to an indoor all-age antheraea pernyi breeding device and method. The indoor all-age antheraea pernyi breeding device comprises a breeding box which is internally provided with ascreen, and a cover plate is movably mounted at the upper end of the breeding box. The side wall of the cover plate is provided with a convex fastener, the cover plate is provided with a plurality ofvent holes, and a plurality of positioning rods are fixedly connected to the lower end of the cover plate. The indoor all-age antheraea pernyi breeding device and method have advantages that antheraea pernyi breeding under the non-manual antheraea pernyi garden conditions is greatly facilitated, antheraea pernyi breeding areas are expanded, influences on growth of antheraea pernyi under conditions of outdoor environments, biological factors and the like are avoided, and the antheraea pernyi reduction ratio is sharply decreased; in addition, silkworm excrement can be collected conveniently, and the economic value in antheraea pernyi breeding is increased while labor cost is reduced.
Owner:HUAIBEI NORMAL UNIVERSITY
Preparation and application of antibiotic peptide pichia yeast
InactiveCN1158382CLow costOptimizing Media FormulationFungiAnimal feeding stuffBiotechnologyFeed additive
The antheraea pernyi antibacterial peptide possesses broad-spectrum bacteriocidal action. According to amino acid sequences of 1-11 situs of natural antibacterial peptide A and amino acid sequences of 12-37 situs of antibacterial peptide D the antibacterial peptide AD gene is designed, and cloned in Pichia yeast and expressed. By optimizing formula of culture medium and culture condition the high-density culture and high-efficiency expression (500 unit / ml) can be obtained in a fermentation tank. The antibacterial peptide yeast product can be added in the feed for piglet in 5000 unit / kg or added in the drinking water for chicken in 60-100 unit / animal. day., can be used for preventing and curing animal diarrhea, and can be used as feed additive instead of partial vitamins.
Owner:SOUTH CHINA AGRI UNIV
Method for preparing gene serial number of lysozyme of tussah, and expression production
This invention discloses a method for cloning Antheraea pernyi lysozyme gene and producing its expression product. This coding region of Antheraea pernyi lysozyme gene contains 420 base pairs, while the coding product (with predicted molecular weight of 13986, and isoelectric point of 8.46) contains 140 amino acid residues that include a signal peptide sequence (for 20 amino acid residues) and a mature peptide sequence (120 amino acid residues). The expression product is produced by inserting the gene fragment of the mature peptide into a yeast expression vector, transforming, screening, culturing, separating and purifying with chromatography.
Owner:辽宁省农业科学院大连生物技术研究所
Antheraea pernyi larva low-temperature stress induced resistance breeding method
ActiveCN114027269ASolve the serious problem of tussah silkworm diseaseSolve serious problemsAnimal husbandryAnimal scienceGenetic stock
The invention discloses an antheraea pernyi larva low-temperature stress induced resistance breeding method, and relates to the technical field of antheraea pernyi breeding. The method comprises the following step: S1, parent matching: carrying out parent screening, namely selecting high-yield, high-quality and high-feed conversion efficiency Liao silkworm 582 as a female parent, and selecting an antheraea pernyi variety with strong disease resistance as a male parent, and preparing the selected female parent and male parent into a hybrid combination. According to the method, the modes of excellent variety hybridization and excellent variety selection are set, low-temperature stress screening pressure is applied to the antheraea pernyi larva by simulating low-temperature cold damage climate conditions on the basis of manual selection from the F2 generation, and 6 generations are applied so that the method has higher reliability and pertinence aiming at the breeding target; and therefore, the problem that tussah diseases are serious due to low temperature and cold damage when the tussah is bred in the field tussah garden is solved, and meanwhile the stability and the high yield of the stocking yield of the tussah in the field tussah garden are guaranteed.
Owner:辽宁省蚕业科学研究所
Antheraea pernyi oil microencapsulation preparation process
InactiveCN102362620AImprove stabilityPrevent oxidative deteriorationEdible oils/fatsSolubilityFood additive
The invention relates to a preparation process for antheraea pernyi oil microcapsules. The preparation process comprises the following steps of: (1) pretreating raw materials; (2) preparing antheraea pernyi oil; and (3) preparing the microcapsules, namely dispersing the antheraea pernyi oil serving as a core material into a water-soluble wall material solution at high speed to form emulsion and performing spray drying to obtain the powdered microcapsule granules. An advanced microencapsulation technology is applied to a processing technology for the antheraea pernyi oil and the antheraea pernyi oil embedded by the microcapsules can avoid oxidative deterioration caused by oxygen, illumination and the like, so that stability is improved, nutritional ingredients and functional ingredients in the antheraea pernyi oil are stable and unchanged, the quality guarantee period is prolonged, solubility is improved, taste and odor are shielded and the like. The antheraea pernyi oil microcapsule products can be applied to food, health-care products, food additives and the like.
Owner:TIANJIN BOHAI VOCATIONAL TECHN COLLEGE
Method for preparing gene serial number of lysozyme of tussah, and expression production
This invention discloses a method for cloning Antheraea pernyi lysozyme gene and producing its expression product. This coding region of Antheraea pernyi lysozyme gene contains 420 base pairs, while the coding product (with predicted molecular weight of 13986, and isoelectric point of 8.46) contains 140 amino acid residues that include a signal peptide sequence (for 20 amino acid residues) and a mature peptide sequence (120 amino acid residues). The expression product is produced by inserting the gene fragment of the mature peptide into a yeast expression vector, transforming, screening, culturing, separating and purifying with chromatography.
Owner:辽宁省农业科学院大连生物技术研究所
Tussah larva hunger-resistant and nutrition stress-resistant breeding method
ActiveCN114698604ASolve the phenomenon of early dryingReduce the high loss rateAnimal husbandryClimate change adaptationNutritionNutrient stress
The invention relates to the technical field of antheraea pernyi breeding, in particular to an antheraea pernyi larva hunger-resistant and nutrition stress-resistant breeding method which comprises the steps of 1, parent screening, 2, parent matching and 3, hybridization breeding. A new tussah variety which has higher adaptability to a bad tussah garden is obtained through hunger resistance and nutrition stress identification verification and a purebred comparison test, the variety has better adaptability and hunger resistance to the tussah garden with old and hard oak leaves, and the comprehensive production performance of the tussah variety reaches the production practical variety level; the phenomena that oak leaves are old and hard and autumn oak trees are dried early due to soil deterioration of the oak garden in the tussah production process are solved, and the problems that the tussah larva loss rate is high, and softening diseases occur frequently are solved.
Owner:辽宁省蚕业科学研究所
Antheraea pernyi guerin-meneville female moth oral liquid capable of maintaining beauty and keeping young and production method of antheraea pernyi guerin-meneville female moth oral liquid
PendingCN113826893APromoting expansionImprove sexual functionNatural extract food ingredientsFood ingredient functionsBiotechnologyAngelica Sinensis Root
The invention belongs to the technical field of health-care food processing, and particularly relates to antheraea pernyi guerin-meneville female moth oral liquid capable of maintaining beauty and keeping young and a production method of the antheraea pernyi guerin-meneville female moth oral liquid. The method comprises the steps of mixing antheraea pernyi guerin-meneville female moths, astragalus membranaceus, angelica sinensis, safflower carthamus, poria cocos, fried peach kernels and radix angelicae, adding white spirit of 50-60 vol, and performing soaking; performing filtering, performing vacuum reduced pressure concentration on filtrate, and controlling the temperature of a mixed solution to be 35-36.5 DEG C and the pressure to be lower than -0.10 MPa to obtain crude antheraea pernyi guerin-meneville female moth liquid; and performing centrifuging in a centrifugal machine, removing block-shaped and drop-shaped fat on the upper layer of a centrifugal cup, performing mixing with a pH regulator and a stabilizer, performing uniform stirring, and performing sterilizing and disinfecting to obtain the antheraea pernyi guerin-meneville female moth oral liquid capable of maintaining beauty and keeping young. According to the method, hormone active substances in the antheraea pernyi guerin-meneville female moths can be fully extracted, the activity of the hormone substances in the product can be kept alive, and nutritional ingredients in the antheraea pernyi guerin-meneville female moths can be fully extracted. The product can regulate female in-vivo estrogen level, improve sleep, enhance immunity, moisten skin, tonify qi and blood, and maintain beauty and keep young.
Owner:中科康吉福(北京)生物科技有限公司
Artificial breeding method of Anastatus orientalis and application in forest pest control
InactiveCN106973866AIncreased parasitic abilityImprove parasitic effectsPlant protectionAnimal husbandryAntheraea pernyiDelicatula
The invention discloses an artificial breeding method of Anastatus orientalis and an application in forest pest control. Ova of Antheraea pernyi are selected as alternative hosts for Anastatus orientalis, and suitable breeding conditions are screened, so that the parasitic capability of Anastatus orientalis is significantly improved. Lycorma delicatula is controlled by means of Anastatus orientalis, and the parasitic effect of Anastatus orientalis on Lycorma delicatula ova is good.
Owner:INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY
An artificial feed for tussah silkworms
ActiveCN104920818BImprove palatabilityNutritional diversityFood processingAnimal feeding stuffAcornAnimal science
The present invention discloses an antheraea pernyi artificial feed which includes the following components: acorn kernel meal, oak leaf powder, multi-vitamin glucose powder, vitamin C and nipagin ester. The mass ratio of each component is as follows: acorn kernel meal 75-85 parts, oak leaf powder 10-20 parts, multi-vitamin glucose powder 0.5-1.0 part, vitamin C 0.05-0.1 part and nipagin ester 0.1-0.2 part. The artificial feed uses the oak tree fruit acorn kernels as main raw materials, and has strong palatability, full nutrition and high feeding rate, can enable young silkworms to develop well, has simple formula, simple manufacturing processes, and low costs, and is easy to promote, etc.
Owner:JILIN SERICULTURE SCI RES INST
A kind of artificial reproduction method of white moth and black stick larvae
Owner:LUOHE CITY FOREST PEST CONTROL & QUARANTINE STATION
Method for increasing utilization rate of antheraea pernyi eggs by trichogramma chilonis
ActiveCN109924170AIncrease profitEasy to produceAnimal husbandryTrichogramma dendrolimiAntheraea pernyi
The invention relates to a method for increasing the utilization rate of antheraea pernyi eggs by trichogramma chilonis. The method specifically comprises the steps that firstly, antheraea pernyi moths are treated to obtain the antheraea pernyi eggs; secondly, parasitic breeding wasps are disinfected and sterilized; when the eclosion rate of the breeding wasps reaches 20-30%, the breeding wasps begin to be inoculated onto the antheraea pernyi eggs, the trichogramma chilonis is inoculated according to the wasp-egg ratio of (7-9):5 at first, and 6-10 h later, trichogramma dendrolimi is inoculated onto the host eggs according to the wasp-egg ratio of (1-3):5; 38-42 h later, adult wasps are removed, the remaining wasps are put into a full dark condition with the temperature of 25+ / -1 DEG C andthe humidity of 80-85% for culture; finally, the eggs are dissected to examine the development states of the wasp bodies, so that storage or putting into use is timely carried out. By means of the method, the utilization rate of the antheraea pernyi eggs in the factory production process of the trichogramma chilonis is effectively increased, the production process of mixed wasps is simplified, the production cost is reduced, and a technical guarantee is provided for the large-scale production and large-area field application of the wasps.
Owner:JILIN ACAD OF AGRI SCI
A set of primers for detection of tussah microsporidia and their application
ActiveCN107400719BRemarkable resultsQuick checkMicrobiological testing/measurementMicroorganism based processesMicrosporidiaMagnetic bead
Owner:辽宁省农业科学院大连生物技术研究所
Antheraea pernyi ovary cell culture medium and application thereof
ActiveCN102618487BComprehensive nutrition for growthEasy to operateVertebrate cellsArtificial cell constructsBiotechnologyPenicillin
The present invention discloses composing components of an antheraea pernyi ovary cell culture medium, a preparation method of the antheraea pernyi ovary cell culture medium, and an application of the antheraea pernyi ovary cell culture medium in antheraea pernyi ovary cell culture. According to the present invention, a prepared amino acid and sugar mixing storage solution (MLM-AAS), an inorganicsalt storage solution (MLM-Salt), a vitamin storage solution (MLM-Vit), bovine serum albumin, fetuin and the like are prepared according to a certain ratio, and the pH value is adjusted to 6.2-6.4 toobtain a MLM-45 culture medium, wherein the prepared MLM-45 culture medium is added with fetal bovine serum and a penicillin and streptomycin mixing solution according to a certain volume ratio before the prepared MLM-45 culture medium is used, the volume ratio of the fetal bovine serum is 20%, and the volume ratio of the penicillin and streptomycin mixing solution is 1%. With a plurality of experiments, the following in vitro cell growth conditions are determined that: the culture temperature is 26-28 DEG C, the pH value is 6.2-6.4, and the osmotic pressure is 315-350 mOsm / kg. With the present invention, technical services are provided for researches in antheraea pernyi biology, antheraea pernyi pathogenic microorganism and related fields.
Owner:辽宁省农业科学院大连生物技术研究所
Tussah moth food and preparation method thereof
ActiveCN105661518BSimple processEasy to operate and implement transformationFood preservationFlavorDesiccant
Owner:JILIN SERICULTURE SCI RES INST
Cationization antheraea pernyi silk fibroin /adenovirus compound, and preparation method and application thereof
PendingCN111575317AHigh infection efficiencyHigh transfection efficiencyOther foreign material introduction processesDsDNA virusesIn vivoGene vector
The invention discloses a cationization antheraea pernyi silk fibroin / adenovirus compound, and a preparation method and application thereof, and belongs to the technical field of biologic medical high molecular materials. The cationization antheraea pernyi silk fibroin is obtained through coupling of antheraea pernyi silk fibroin activated by N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride, and polyethyleneimine, the antheraea pernyi silk fibroin having positive charges on the surface, is coated to the surface of an adenovirus through electrostatic adsorption action, and the cationization antheraea pernyi silk fibroin / adenovirus compound is obtained. The invention constructs a novel cationization antheraea pernyi silk fibroin / adenovirus compound genetic carrier high in infection efficiency, low in cytotoxicity and low in cell targeting properties, in accordance with defects of a naked adenovirus carrier and defects of an existing adenovirus compound carrier, and the cationization antheraea pernyi silk fibroin / adenovirus compound has favorable application prospects in in vivo and vitro infection of target cells.
Owner:SUZHOU UNIV
Screening method and application of tussah breeding material
PendingCN114216870ASolve the problem of low practical hatching rateEasy to operateMicrobiological testing/measurementColor/spectral properties measurementsAnimal scienceAcrosin
The invention belongs to the technical field of silkworm breeding, and particularly relates to a method for screening antheraea pernyi breeding materials and application thereof.The method includes the steps that the antheraea pernyi breeding materials of different varieties are selected, seminal fluid generated in the mating process of male and female seed moths is obtained, acrosomal enzyme activity determination is conducted on the collected and reserved seminal fluid, and after the female moths of the breeding materials of the various varieties lay eggs, the female moths of the breeding materials of the various varieties lay eggs. The practical hatching rate of the tussah eggs of each variety is counted, a regression equation is established according to the relationship between the acrosin enzyme activity of the sperms of each variety and the practical hatching rate of the tussah eggs, and the acrosin enzyme activity of the tussah sperms of different breeding materials can be measured in the'two-generation and one-release 'production period of the tussah. The practical hatching rate of the silkworm eggs is predicted according to the established linear regression equation, so that the male breeding material with the high practical hatching rate of the silkworm eggs is screened out and used for breeding of the'two-stage and one-stage 'tussah varieties, and the male breeding material screened out in the mode can mate to obtain seed eggs with the high practical hatching rate of the tussah eggs.
Owner:辽宁省蚕业科学研究所
A kind of artificial breeding method of Orienta orientalis and its application in preventing and controlling forest pests
InactiveCN106973866BIncreased parasitic abilityImprove parasitic effectsPlant protectionAnimal husbandryZoologyAntheraea pernyi
The invention discloses an artificial breeding method of Anastatus orientalis and an application in forest pest control. Ova of Antheraea pernyi are selected as alternative hosts for Anastatus orientalis, and suitable breeding conditions are screened, so that the parasitic capability of Anastatus orientalis is significantly improved. Lycorma delicatula is controlled by means of Anastatus orientalis, and the parasitic effect of Anastatus orientalis on Lycorma delicatula ova is good.
Owner:INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY
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