30 results about "Antheraea pernyi" patented technology

The invention discloses bio-medical engineering material and preparation method thereof, particularly relates to 3D tissue repair material suitable for adhesively growing of cell and preparation method thereof, belonging to bio-medical material technique domain. The natural tussah silk is used as raw material. After antheraea pernyi silk fibroin dissolves, 3D non-woven web structure composed of antheraea pernyi silk fibroin fibre is obtained by electrostatic spinning, wherein the fibre diameter is 50nm-20 mu m, the hole between the fibres is 1-500 mu m, the thickness of non-woven web is 50nm-20mm, and molecular weight of the antheraea pernyi silk fibroin is 10X10<4>-20X10<5>D. The bio-medical engineering material has features of non-poison, harmless, good biocampatibility, good adhesive growth ability of cell, etc. Because of the wide materials supply and low cost, the said material will replace some high-cost bio-medical material such as collagen, and will be used for repair material such as artificial skin, tendon, cartilage, duramater and so on, particularly for histiocyte induction material.

The invention discloses an artificial multiplication method for tetrastichus septentrionalis. The method includes the following steps of (1) selection of a substitute host; (2), field collection and selection of micromelalopha troglodyta pupa; (3), cultivation of bee species; (4) extraction of information matter; (5), processing of antheraea pernyi pupa; (6), bee receiving; (7), cultivation and development. According to the artificial multiplication method, pupa which is not subjected to parasitism is soaked with ethyl alcohol, pupa surface extract is obtained, then the pupa surface extract is sprayed on the antheraea pernyi pupa in an artificial spraying mode, the tetrastichus septentrionalis is induced to be subjected to parasitism, and the parasitism rate is raised. The tetrastichus septentrionalis is subjected to indoor large-scale multiplication, and a practical technical means is provided for preventing and controlling hyphantria cunea, micromelalopha troglodyta and other lepidoptera pests for forest creatures.

The invention relates to a method for preparing Antheraea Pernyi powder and Antheraea Pernyi powder thereof. The method comprises the following steps: heating cocoons of Antheraea Pernyi to obtain moths, spawning, cleaning and disinfecting the Antheraea Pernyi, refrigerating and freezing, heating at the temperature of -45 DEG C under 0.01MPa-0.09MPa for 2-5 DEG C/hour to the temperature of 30 DEG C-40 DEG C, insulating for 2-3 hours and then refrigerating, vacuum drying, crushing in a low temperature pulverizer at the low temperature of 0 DEG C- -35 DEG C to 100 meshes and sieving. The main quality index of the Antheraea Pernyi powder are: nucleic acid (mg/g) >=30.0, free amino acid (mg/g) >=12.0, VE (ug/g) >=2.0, bacterium colony total amount (CFU/g) <=3000. The invention is characterized in that the low temperature pulverization is employed for directly processing the fresh oophyte which greatly reserves the active component of Antheraea Pernyi and is suitable for the requirements of high-grade health product and medicine production. The particle degree of the ultrafine powder at low temperature reaches more than 100 meshes, so that the biological utilization degree can be enhanced and the medicinal dosage forms such as hard capsule can be conveniently processed. The method has the advantages of simple technology and convenient operation, the product has the advantages of easy preservation and convenient application which solves the problems of transportation and fresh-preservation, the product also has the merits of moderate cost, high benefit and wide market prospect.

The present invention discloses an antheraea pernyi artificial feed which includes the following components: acorn kernel meal, oak leaf powder, multi-vitamin glucose powder, vitamin C and nipagin ester. The mass ratio of each component is as follows: acorn kernel meal 75-85 parts, oak leaf powder 10-20 parts, multi-vitamin glucose powder 0.5-1.0 part, vitamin C 0.05-0.1 part and nipagin ester 0.1-0.2 part. The artificial feed uses the oak tree fruit acorn kernels as main raw materials, and has strong palatability, full nutrition and high feeding rate, can enable young silkworms to develop well, has simple formula, simple manufacturing processes, and low costs, and is easy to promote, etc.

The invention provides an artificial feed formula for cantheconidae furcellata. The artificial feed formula for cantheconidae furcellata comprises 10-30 parts by weight of maggot meal, 10-20 parts byweight of bovine livers, 5-15 parts by weight of yolk, 5-17.5 parts by volume of antheraea pernyi haemolymph, and 3.5-8 parts by weight of honey. The artificial feed formula can be used for feeding cantheconidae furcellata in replacement of tenebrio molitor; moreover, the artificial feed formula is capable of ensuring normal growth and development of cantheconidae furcellata, as well as improvingnymph survival rate and offspring hatch rate of the cantheconidae furcellata.

The invention discloses a construction method and application of an antheraea pernyi multinucleocapsid nucleopolyhedrovirus shuttle vector, and belongs to the field of a biological technology. A bacterium artificial chromosome cloning vector is used for constructing a transfer vector comprising a DNA fragment containing a lacZ alpha report gene and a bacterial transposon Tn7 transposition target point sequence, a DNA fragment containing an AnpeNPV polyhedrin gene promoter sequence and an upstream gene partial sequence, and a DNA fragment containing a polyhedrin gene 3' terminal partial codingregion sequence and a downstream gene partial sequence, and linearized transfer vector plasmid DNA and linearized AnpeNPV genomic DNA are subjected to cotransfection with Tn-High Five cells, so that recombinant AnpeNPV is obtained. The recombinat AnpeNPV genomic DNA is transformed to escherichia coli, so that an AnpeNPV shuttle vector AnpeNPV-bacmid is obtained. The shuttle vector can be reproduced in the manner of plasmid DNA in the escherichia coli, can also be reproduced in the virus DNA manner in Tn-High Five insect cells and antheraea pernyi, and has infectivity.

The invention relates to the field of silk fibroin extraction, and discloses an extraction method of wild antheraea pernyi silk fibroin. The extraction method comprises the following steps: degumming and drying tussah silks; extracting the wild antheraea pernyi silk fibroin with a mixed solution system of FeCl2 and tetrasodium iminodisuccinate, and adjusting pH under ultrasonic assistance in an extracting process in order to increase the extraction rate; performing ultrafiltration on a solution undergoing ultrasonic treatment, performing freeze drying on the remaining solution after ultrafiltration, and grinding the obtained wild antheraea pernyi silk fibroin into powder for later use. The method provided by the invention is easy to operate, and has low environmental harm and high protein extraction rate; the obtained silk fibroin has higher purity.

The invention relates to an indoor all-age antheraea pernyi breeding device and method. The indoor all-age antheraea pernyi breeding device comprises a breeding box which is internally provided with ascreen, and a cover plate is movably mounted at the upper end of the breeding box. The side wall of the cover plate is provided with a convex fastener, the cover plate is provided with a plurality ofvent holes, and a plurality of positioning rods are fixedly connected to the lower end of the cover plate. The indoor all-age antheraea pernyi breeding device and method have advantages that antheraea pernyi breeding under the non-manual antheraea pernyi garden conditions is greatly facilitated, antheraea pernyi breeding areas are expanded, influences on growth of antheraea pernyi under conditions of outdoor environments, biological factors and the like are avoided, and the antheraea pernyi reduction ratio is sharply decreased; in addition, silkworm excrement can be collected conveniently, and the economic value in antheraea pernyi breeding is increased while labor cost is reduced.

The antheraea pernyi antibacterial peptide possesses broad-spectrum bacteriocidal action. According to amino acid sequences of 1-11 situs of natural antibacterial peptide A and amino acid sequences of 12-37 situs of antibacterial peptide D the antibacterial peptide AD gene is designed, and cloned in Pichia yeast and expressed. By optimizing formula of culture medium and culture condition the high-density culture and high-efficiency expression (500 unit/ml) can be obtained in a fermentation tank. The antibacterial peptide yeast product can be added in the feed for piglet in 5000 unit/kg or added in the drinking water for chicken in 60-100 unit/animal. day., can be used for preventing and curing animal diarrhea, and can be used as feed additive instead of partial vitamins.

This invention discloses a method for cloning Antheraea pernyi lysozyme gene and producing its expression product. This coding region of Antheraea pernyi lysozyme gene contains 420 base pairs, while the coding product (with predicted molecular weight of 13986, and isoelectric point of 8.46) contains 140 amino acid residues that include a signal peptide sequence (for 20 amino acid residues) and a mature peptide sequence (120 amino acid residues). The expression product is produced by inserting the gene fragment of the mature peptide into a yeast expression vector, transforming, screening, culturing, separating and purifying with chromatography.

The invention relates to the technical field of antheraea pernyi breeding, in particular to an antheraea pernyi larva hunger-resistant and nutrition stress-resistant breeding method which comprises the steps of 1, parent screening, 2, parent matching and 3, hybridization breeding. A new tussah variety which has higher adaptability to a bad tussah garden is obtained through hunger resistance and nutrition stress identification verification and a purebred comparison test, the variety has better adaptability and hunger resistance to the tussah garden with old and hard oak leaves, and the comprehensive production performance of the tussah variety reaches the production practical variety level; the phenomena that oak leaves are old and hard and autumn oak trees are dried early due to soil deterioration of the oak garden in the tussah production process are solved, and the problems that the tussah larva loss rate is high, and softening diseases occur frequently are solved.

The invention belongs to the technical field of health-care food processing, and particularly relates to antheraea pernyi guerin-meneville female moth oral liquid capable of maintaining beauty and keeping young and a production method of the antheraea pernyi guerin-meneville female moth oral liquid. The method comprises the steps of mixing antheraea pernyi guerin-meneville female moths, astragalus membranaceus, angelica sinensis, safflower carthamus, poria cocos, fried peach kernels and radix angelicae, adding white spirit of 50-60 vol, and performing soaking; performing filtering, performing vacuum reduced pressure concentration on filtrate, and controlling the temperature of a mixed solution to be 35-36.5 DEG C and the pressure to be lower than -0.10 MPa to obtain crude antheraea pernyi guerin-meneville female moth liquid; and performing centrifuging in a centrifugal machine, removing block-shaped and drop-shaped fat on the upper layer of a centrifugal cup, performing mixing with a pH regulator and a stabilizer, performing uniform stirring, and performing sterilizing and disinfecting to obtain the antheraea pernyi guerin-meneville female moth oral liquid capable of maintaining beauty and keeping young. According to the method, hormone active substances in the antheraea pernyi guerin-meneville female moths can be fully extracted, the activity of the hormone substances in the product can be kept alive, and nutritional ingredients in the antheraea pernyi guerin-meneville female moths can be fully extracted. The product can regulate female in-vivo estrogen level, improve sleep, enhance immunity, moisten skin, tonify qi and blood, and maintain beauty and keep young.

The invention discloses an artificial breeding method of Anastatus orientalis and an application in forest pest control. Ova of Antheraea pernyi are selected as alternative hosts for Anastatus orientalis, and suitable breeding conditions are screened, so that the parasitic capability of Anastatus orientalis is significantly improved. Lycorma delicatula is controlled by means of Anastatus orientalis, and the parasitic effect of Anastatus orientalis on Lycorma delicatula ova is good.

The invention relates to a method for increasing the utilization rate of antheraea pernyi eggs by trichogramma chilonis. The method specifically comprises the steps that firstly, antheraea pernyi moths are treated to obtain the antheraea pernyi eggs; secondly, parasitic breeding wasps are disinfected and sterilized; when the eclosion rate of the breeding wasps reaches 20-30%, the breeding wasps begin to be inoculated onto the antheraea pernyi eggs, the trichogramma chilonis is inoculated according to the wasp-egg ratio of (7-9):5 at first, and 6-10 h later, trichogramma dendrolimi is inoculated onto the host eggs according to the wasp-egg ratio of (1-3):5; 38-42 h later, adult wasps are removed, the remaining wasps are put into a full dark condition with the temperature of 25+/-1 DEG C andthe humidity of 80-85% for culture; finally, the eggs are dissected to examine the development states of the wasp bodies, so that storage or putting into use is timely carried out. By means of the method, the utilization rate of the antheraea pernyi eggs in the factory production process of the trichogramma chilonis is effectively increased, the production process of mixed wasps is simplified, the production cost is reduced, and a technical guarantee is provided for the large-scale production and large-area field application of the wasps.

The invention discloses a nosema pernyi detection primer and application thereof. Specifically, the base sequence of the primer is shown in SEQ ID NO. 1-3, and a template extraction method of the primer comprises the step of releasing, enrichment, separation and purification of DNA through alkaline lysis and magnetic beads. The primer is good in specificity and high in sensitivity; and according to a nosema pernyi detection method established through the primer, operation is simple and rapid, the cost is low, the flux is high, living pupa sampling can be achieved, the antijamming capability is high, the contamination risk is low, the quality of extracted DNA is high, and automatic extraction can be achieved. The method can be widely applied to molecular detection, the detection flux and the operation convenience can be remarkably improved, the false positive rate of detection can be obviously decreased, and the detection sensitivity and accuracy and the detection efficiency are improved. The nosema pernyi detection primer and the application thereof have important significance for centralized quarantine of antheraea pernyi granulosis and conservation of genetic resources in the antheraea pernyi industry.

The invention discloses a cationization antheraea pernyi silk fibroin /adenovirus compound, and a preparation method and application thereof, and belongs to the technical field of biologic medical high molecular materials. The cationization antheraea pernyi silk fibroin is obtained through coupling of antheraea pernyi silk fibroin activated by N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride, and polyethyleneimine, the antheraea pernyi silk fibroin having positive charges on the surface, is coated to the surface of an adenovirus through electrostatic adsorption action, and the cationization antheraea pernyi silk fibroin /adenovirus compound is obtained. The invention constructs a novel cationization antheraea pernyi silk fibroin /adenovirus compound genetic carrier high in infection efficiency, low in cytotoxicity and low in cell targeting properties, in accordance with defects of a naked adenovirus carrier and defects of an existing adenovirus compound carrier, and the cationization antheraea pernyi silk fibroin /adenovirus compound has favorable application prospects in in vivo and vitro infection of target cells.

The invention belongs to the technical field of silkworm breeding, and particularly relates to a method for screening antheraea pernyi breeding materials and application thereof.The method includes the steps that the antheraea pernyi breeding materials of different varieties are selected, seminal fluid generated in the mating process of male and female seed moths is obtained, acrosomal enzyme activity determination is conducted on the collected and reserved seminal fluid, and after the female moths of the breeding materials of the various varieties lay eggs, the female moths of the breeding materials of the various varieties lay eggs. The practical hatching rate of the tussah eggs of each variety is counted, a regression equation is established according to the relationship between the acrosin enzyme activity of the sperms of each variety and the practical hatching rate of the tussah eggs, and the acrosin enzyme activity of the tussah sperms of different breeding materials can be measured in the'two-generation and one-release 'production period of the tussah. The practical hatching rate of the silkworm eggs is predicted according to the established linear regression equation, so that the male breeding material with the high practical hatching rate of the silkworm eggs is screened out and used for breeding of the'two-stage and one-stage 'tussah varieties, and the male breeding material screened out in the mode can mate to obtain seed eggs with the high practical hatching rate of the tussah eggs.