1138 results about "Bombyx mori" patented technology

The invention discloses a method of preparing a bombyx mori silk fibroin specific antibody by utilizing a characteristic polypeptide. The method comprises the following steps: synthesizing a "CGAGAGSGAGAGS" polypeptide sequence by utilizing an Fmoc method, coupling the polypeptide with keyhole limpet hemocyanin (KLH) through the cysteine on the N terminus of the polypeptide so as to obtain a complete antigen; diluting the complete antigen with normal saline, mixing the diluted complete antigen with a complete Freund's adjuvant, adding streptomycin and penicillin to carry out an emulsion treatment so as to obtain a primary immunized antigen emulsion, subjecting a rabbit to a primary immunization by using the primary immunized antigen emulsion, then subjecting the rabbit to a strengthened immunization, wherein the strengthened immunization uses a strengthened immunized antigen emulsion, which is prepared by the following steps: mixing the diluted complete antigen with an incomplete Freund's adjuvant, and then adding streptomycin and penicillin to carry out an emulsion treatment so as to obtain the target product; collecting the blood of the immunized rabbit, when the antibody titer in the rabbit blood sample reaches 1/10000; making the blood blocks fully contract to completely separate out the antiserum, then collecting the antiserum, and subjecting the antiserum to a centrifugation treatment so as to obtain a supernate.

The invention discloses a lifting silkworm cluster and a method for cocooning by adopting the silkworm cluster. Blow-pipe bamboo clusters or paper cocooning frames are uniformly arranged on a liftable framework side by side, the framework is formed by fixedly connecting at least two cross beams and two longitudinal beams, and the framework is artificially lifted by pulling a rope through a pulley block. When silkworms climb the cluster for cocooning, the rope is reeled off to lower the lower paper cocooning frames to be close to the silkworm bodies, and the matured silkworms naturally climb upwards for spinning and cocooning when meeting the paper cocooning frames or the blow-pipe bamboo clusters; and after the silkworms finish cocooning, the rope is pulled to make the silkworm cluster leave the ground for about 2 meters, and silkworm excrement in a silkworm room can be immediately cleared. By adopting the lifting silkworm cluster for cocooning, time and labor are saved, the space of the silkworm room is fully utilized, the quality of silkworm cocoons is greatly improved, the service life of the cluster tool is prolonged, and the labor efficiency of silkworm raisers is greatly improved.

The invention provides a method for efficiently preparing stachyose from stachys sieboldii. The method is characterized by comprising the following steps: taking Chinese traditional vegetable stachys sieboldii as a raw material; extracting the stachyose by adopting a pure water homogenate method; and spraying and drying after the processes of filtering a homogenate by a cloth bag; decolorizing the filtered homogenate with macroporous resin, performing ultrafiltration purification and reverse osmosis concentration, and the like, wherein a reverse osmosis permeate liquid is used as water for the homogenate and the resin decolonization elution in the production and the like, so not only resources can be used fully to meet the requirement on production water supply, but also the influence on the purity of the stachyose in the whole production system, caused by pollutants such as saline matters and the like in a natural water source, can be reduced to further improve the purity and the quality of the stachyose. By using the method of the invention, the extraction rate of the stachyose prepared can reach 75 and 85 percent, the purity of the stachyose in a product reaches over 90 percent, and the total recovery rate of the stachyose in the whole process reaches over 60 percent. The method combines a traditional extraction method and a modern separation method, has a simple process and a large handling capacity, is suitable for industrial production, and simultaneously has high product purity, saves resources and the production cost, and has good economic benefits and ecological benefits.

The invention discloses an organic leaf surface fertilizer rich in organic selenium and vitamins and a preparation method for the organic leaf surface fertilizer. The organic leaf surface fertilizer rich in the organic selenium and the vitamins comprises the raw materials in parts by weight: 40-60 parts of selenium-enriched bombyx mori, 40-60 parts of selenium-enriched plant waste, 40-60 parts of selenium-enriched edible mushroom waste, 40-60 parts of sodium selenite, 20-30 parts of vitamins and 1-10 parts of a composite fungicide. The selenium-enriched bombyx mori, the selenium-enriched plant waste and the selenium-enriched edible mushroom waste of the fertilizer can supply selenium elements and also supply animal proteins, plant proteins and microorganism proteins and are fermented by composite bacteria to polypeptide combined with the selenium elements. Therefore, the fertilizer can be used for supplying enough organic selenium and beneficial substances such as microorganisms and probiotics to the growth of crops; a large amount of waste is turned into treasures, so that social and natural resources are saved, the environment is protected, and cyclic economy is realized.

A method for expressing antigens of foot-and-mouth disease virus in insect in vivo by using re-combinant baculovirus is disclosed, which comprises: cloning different genes combination of foot-and-mouth disease virus into baculovirus carrying vector to construct transfer vector, transfecting baculovirus with the transfer vector to proceed DNA recombination, and thus to get recombinant baculovirus, infecting insect host with the recombinant baculovirus, culturing the infected insect host to express the antigens of foot-and-mouth disease virus, and then collecting and purifying the expressed antigens. The preferred genes, baculovirus carrying vector, baculovirus and insect host are selected from P1-2A3C, ORF and VP1 genes of foot-and-mouth disease virus as shown in SEQ ID NO 1., 3 or 5, pVL1393, Bombyx mori Bm-NPV-ZJ8 and larvae or pupa of Bombyx mori, respectively.

The invention discloses hundred-herb traumatic injury ointment and a preparation method thereof. The ointment is prepared from hundreds of medicinal materials including rhizoma polygoni cuspidati, sambucus javanica reinw, kusnezoff monkshood root, pinellia ternata, India madder root, cortex eucommiae, radix dipsaci, radix achyranthis bidentatae, rhizoma drynariae, saffron crocus, litharge, radix rehmanniae recens, semen momordicae, radix saposhnikoviae, rhubarb, fructus aurantii, peach seed, frankincense, centipede, Qi snake, cornu cervi pantotrichum, ground beetle, pangolin scales, long-noded pit viper, garter snake, snake slough, scorpion, Egeria najas, stiff silkworm and the like. The hundred-herb traumatic injury ointment has a better effect by combining the animal and plant materials; and when being applied to traumatic injuries, the ointment can quickly relieve swelling and pain, activate blood and remove fatigue, relax sinew and activate collaterals, treat fracture dislocation, prevent relapse and sequela, and realize radical treatment.

The invention relates to a panus giganteus culture medium. The panus giganteus culture medium is prepared by the following raw materials in parts by weight: 28-35 parts of broadleaf sawdust, 15-20 parts of wheat bran, 7-14 parts of soybean curd residue, 3-5 parts of monosodium glutamate mycoprotein, 5-10 parts of semen litchi particles, 12-15 parts of pineapple peels, 4-6 parts of silkworm excrement, 13-18 parts of dry poultry manure, 5-8 parts of pine needle meal, 1-2 parts of montmorillonite powder, 1-2 parts of calcined lime, 0.5-1 part of calcium superphosphate, 1-2 parts of kieselguhr and 5-10 parts of nutrition additives. According to the culture medium, sources of main materials are extensive, the cost is low, various nutrient elements are adopted, components are rich; the culture medium is synergetic with the nutrition additives and is easily absorbed by the thallus, so that the biotransformation efficiency is improved, and the nutrition is rich; compared with the normal culture medium, the panus giganteus culture medium has the advantages that the yield of the thallus in per kilogram of the culture medium is increased by 60-75%; the cultured thallus is thick and strong, and strong in stress resistance.

The invention relates to a method for producing big Clitocybe by using mulberry stems, sugarcane bagasse and silkworm excrement. The mulberry stems and sugarcane bagasse are common agricultural residue in Guangxi; the mulberry stems, the sugarcane bagasse and the silkworm excrement are prepared into a cultivation material; fermentation is performed on the cultivation material through mixed bacteria, degradation of organic matters of the raw materials can be accelerated, and conversion of the organic matters is fast; degradation of organic matters is promoted, crude fiber of the mulberry stems and the sugarcane bagasse can be fully utilized to improve the nutriment content of the cultivation material, and high yield of the big Clitocybe can be achieved; and the quality of the big Clitocybe can be improved through organic nutriments of the silkworm excrement, and the economic benefits can be increased.

The invention discloses a Chinese herbal medicine feed additive for improving disease resistance of wild Queshan chicken. The feed additive is mainly prepared from the following raw material medicines in part by weight: 5 to 15 parts of nux vomica, 3 to 10 parts of medicated leaven, 2 to 5 parts of southernwood, 2 to 5 parts of wild chrysanthemum, 2 to 5 parts of folium eucalypti, 5 to 10 parts of almond, 5 to 10 parts of phellodendron, 2 to 5 parts of portulaca oleracea, 2 to 5 parts of kuh-seng, 3 to 8 parts of common lophatherum herb, 3 to 8 parts of indigowoad leaf, 3 to 8 parts of foliumperillae, 2 to 5 parts of mint, 2 to 5 parts of liquorice, 2 to 5 parts of weeping forsythia, 3 to 8 parts of indigowoad root, 5 to 10 parts of honeysuckle, 2 to 5 parts of virgate wormwood herb, 2 to 5 parts of Chinese angelica, 2 to 5 parts of kudzu-vine root, 1 to 3 parts of baical skull cap root, 1 to 3 parts of goldthread, 3 to 8 parts of white paeony root, 3 to 8 parts of costustoot, 1 to 3parts of wrinkled gianthyssop herb, 3 to 8 parts of tangerine peel, 1 to 3 parts of Chinese thorowax root, 1 to 3 parts of stiff silkworm, 2 to 5 parts of figwort root and 1 to 3 parts of angelica dahurica.

The invention relates to a method for a silkworm diapause variety low temperature egg incubation transgene. Silkworm diapause variety next generation graine voltinism is changed in the special egg incubation condition, to ensure that present generation silkworm diapause variety moths obtained under the special egg incubation condition mate, to give birth to non-diapause next generation graine, finally referring to the microinjection transgene method of the non-diapause silkworm eggs embryo, the gene transferring is performed, therefore the obstruction of the silkworm diapause variety transgene technology is expertly broken, and a transgene silkworm is effectively manufactured. The method directly adopts the universal use utility silkworm diapause variety as original material in production, can avoid the long and troublesome breeding process, and directly utilizes the transgene technology to create the good new special silkworm variety with various characteristics, the breeding cycle can be greatly shortened, and massive manpower and physical resources are saved.

The invention discloses efficient organic fertilizer and a preparation method thereof. The efficient organic fertilizer comprises, by weight, 15-22 parts of mushroom dreg, 8-13 parts of brewer's grains, 18-20 parts of riverway sludge, 5-7 parts of ardealite, 22-25 parts of alternanthera philoxeroides, 20-23 parts of pueraria phaseoloides, 18-20 parts of azolla, 18-20 parts of enteromorpha, 18-20 parts of seaweed, 20-28 parts of biogas residues, 26-30 parts of silkworm feces, 28-33 parts of sheep manure, 30-32 parts of overdue milk, 5-8 parts of milk powder and 40-60 parts of peat. The organic fertilizer has the advantages of improving soil properties, improving soil fertility and increasing crop output.

The invention relates to the technical field of fertilizers, in particular to an ecological organic foliar fertilizer. The foliar fertilizer is mainly prepared from, by weight, 10 parts of oil tea cake powder, 25 parts of soybean meal, 20 parts of pond mud, 6 parts of Japanese cayratia herb, 5 parts of herba hydrocotylis, 7 parts of moss, 3 parts of herba portulacae, 2 parts of common threewingnut roots, 15 parts of silkworm excrement, 30 parts of chicken manure, 20 parts of pig manure, 5-10 parts of organic refuse, 250-400 parts of water, 200-250 parts of biogas slurry and 3-5 parts of an EM bacterium stock solution. The ecological organic foliar fertilizer prepared by carefully selecting the raw materials and the optimal proportion thereof is rich and comprehensive in nutrition, significant in fertilizer effect, good in absorption, capable of rapidly achieving the effects, improving the growth condition of crops, enhancing the pest and disease damage resistance of the crops and not causing environmental pollution and good in degradability, has a broad-spectrum fungicidal effect, an antifungal effect, a fungus inhibiting effect and the like and can be widely applied to planting of the crops, economic crops, woods, traditional Chinese medicinal materials and the like.

The invention discloses a method for synthetizing secretion lysozyme by middle silkgland cell of silkworm. The method comprises the following steps: building a pBSer1hLYZ (lysozyme)-A3EGFP (Enhanced Green Fluorescent Protein) plasmid for synthetizing secretion lysozyme by the silkworm; then, introducing the plasmid and an assistant plasmid capable of providing transposase into a silkworm germ cell according to the microinjection transgenosis silkworm technology; according to the transposition characteristics of a piggyBac transposon, introducing a green fluorescent protein gene and a lysozymegene into a silkworm gene group, to obtain stable heredity and expression so as to create the transgenosis silkworm capable of synthetizing secretion lysozyme by middle silkgland cell of silkworm by specificity; further, hybridizing the transgenosis silkworm with sericin silkworm; carrying out back crossing on the hybridized descendant with the sericin silkworm for 3-5 generations; and finally, carrying out selfing on the obtained product to carry out homozygosis on the lysozyme gene so as to obtain the new species of the transgenosis silkworm of the secretion lysozyme. According to the method, a basis for improving lysozyme production efficiency and lowering production cost is laid.

The invention relates to a silkworm diapause variety early pickling transgene method. The hydrochloric dipping treatment is performed to a silkworm diapause ovum, by utilizing a certain time before silkworm egg embryo is developed to a germ band formed, namely, before 2 hours and a half after egg casting, then the embryo microinjection is performed to the graine of diapause treatment canceling, the G0 generation silkworm obtained from the incubation breeding bred to eclosion, the G1 generation silkworms are produced through the idiogamy or the backcross of the silkworms, and labeled and sieved through fluorescence, and therefore the transgene silkworms are effectively manufactured. The method directly adopts the universal use utility silkworm diapause variety as original material in production, can avoid the long and troublesome breeding process, and directly utilizes the transgene technology to create the good new special silkworm variety with various characteristics, the breeding cycle can be greatly shortened, and massive manpower and physical resources are saved.

The invention provides a method for making transgenic silkworms of a silkworm diapause variety. The method comprises the steps of utilizing the relationship between the environmental conditions of a parental generation and the diapause property of filial-generation silkworm eggs and utilizing a generation before transgenic operation to control the environmental conditions of the diapause silkworm variety so as to change the diapause property of a present generation of transgenic operation, performing silkworm-egg embryo microinjection on non-diapause silkworm eggs, utilizing fluorescent protein labeling to screen and making the transgenic silkworms. The method has the advantages of ingeniously breaking through the obstacles of transgenic technology for the silkworm diapause variety and effectively overcoming the defects of the prior transgenic method, and can lay a solid technical foundation for the large-scale application of silkworm transgenic technology in functional gene research, bioreactor development and other practical research.

Transgenic silkworms comprising at least one nucleic acid encoding a chimeric silk polypeptide comprising one or more spider silk elasticity and strength motifs are disclosed. Expression cassettes comprising nucleic acids encoding a variety of chimeric spider silk polypeptides (Spider 2, Spider 4, Spider 6, Spider 8) are also disclosed. A piggyBac vector system is used to incorporate nucleic acids encoding chimeric spider silk polypeptides into the mutant silkworms to generate stable transgenic silkworms. Chimeric silk fibers having improved tensile strength and elasticity characteristics compared to native silkworm silk fibers are also provided. The transgenic silkworms greatly facilitate the commercial production of chimeric silk fibers suitable for use in a wide variety of medical and industrial applications.

The invention provides a method for selecting and breeding a novel silkworm breed capable of producing a great number of all natural pure sericin proteins, which introduces a special germ plasm resource from Japan, adopts genetic thremmatology to be combined with bioscience, and utilizes a distant hybridization method to breed a silkworm breed with sturdy, disease prevention, high temperature resistance, short breeding period and excellent economic character. The method leads excellent genes of the silkworm breed in the germ plasm resource to breed into an all natural pure sericin silkworm breed with high purity, and provides a novel approach for non spun silk industries to produce all natural pure siricin materials.

A method for preparing a sericin hydrogel, the method including: 1) weighing a cocoon of a fibroin-deficient mutant silkworm, Bombyx mori, extracting the cocoon by an aqueous solution of LiBr or LiCl, dialyzing an extracted solution to yield a sericin solution having a concentration of a non-degraded sericin of between 0.1 and 4 wt. %; and 2) concentrating the sericin solution to a concentration of between 1.5 and 10 wt. %, adding a crosslinking agent to the concentrated sericin solution at a ratio of between 2 and 500 μL of the crosslinking agent per each milliliter of the sericin solution, fully blending the crosslinking agent with the concentrated sericin solution, and keeping a resulting mixture at the temperature of between 4 and 45° C. for between 5 s and 36 hrs to yield a hydrogel.

The invention relates to a Chinese herbal medicine additive, especially a Chinese herbal medicine additive for fattening pig feed. The Chinese herbal medicine additive provided by the invention is made of the following components of: by weight, 14-16% of haw, 8-10% of Radix Astragali, 4-6% of honeysuckle flower, 9-11% of epimeddium, 8-10% of muscardine silkworm, 14-16% of black sesame, 14-16% of a pine needle powder, 8-10% of common fennel fruit and 8-10% of Mythic Fungus. The Chinese herbal medicine additive for fattening pig feed has functions of strengthening stomach, disinhibiting dampness, eliminating indigestion and stagnation, clearing heat and detoxifying and improving immunity. After fattening pigs eat the pig feed added with the Chinese herbal medicine additive, the lean meat percentage, eye muscle area and average daily weight gain of fattening pigs are all remarkably increased; in the meanwhile, the feed/gain ratio and average backfat thickness are both remarkably reduced.

The invention relates to a disinfectant for culturing silkworms, which uses bromochlorohydantoin as a main agent and an alkaline sodium salt as an auxiliary agent, wherein when the disinfectant is prepared into solution by using water as a solvent, the mass volume density of the bromochlorohydantoin is 120 to 960 mg/L, and the mass percentage concentration of the auxiliary agent is 0.25 to 0.75 percent. When the disinfectant is prepared into powder, the mass percentage of the bromochlorohydantoin is 5 to 20 percent, and the mass percentage of the corresponding auxiliary agent is 80 to 95 percent. The alkaline sodium salt is any one of trisodium phosphate, chlorinated trisodium phosphate or sodium bicarbonate. More preferably, the mass volume concentration of the bromochlorohydantoin is 240 to 480 mg/L, and the mass percentage concentration of the alkaline sodium salt is 0.45 to 0.5 percent. The disinfectant develops novel application of the existing medicament bromochlorohydantoin; the disinfectant prepared from the bromochlorohydantoin and an alkaline compound of the trisodium phosphate, the chlorinated trisodium phosphate or the sodium bicarbonate and the like is safe and reliable, has a good effect of killing main pathogens of NPV, CPV, N. b, beauveria bassiana, aspergillus flavus, bacillus thuringiensis and toxins, and is an effective broad-spectrum disinfectant for culturing the silkworms.

The invention discloses a method for the three-dimensional and artificial seedlings cultivation of the perinereis aibuhitensis, which mainly comprises the steps of: (1) fishing and disinfecting the heteronereis; (2) laying eggs, hatching and cultivating the planktonic larva; (3) three-dimensionally cultivating the benthic larvae; and (4) seedling emerging. The key measures comprise the steps of: putting four setiger intermediate-later stage nectochaete into a polyethylene water tank for the three-dimensional cultivation, cultivating by flowing water, and properly mixing the single-cell algae, the EM (effective microorganisms) bacterium with various artificial baits. After the measures are used, the density of the larvae is reduced, the mutual massacre among the individuals is reduced, the nutritional requirement of each-stage larva and the good and stable water quality environment are guaranteed, the survival rate of the larva is greatly improved, the breeding rate from the fertilized ovum to the sand silkworm seedling with the length of 1cm reaches at 42.3%, and the seedling yield per unit area is improved at 11.4*104 numbers/m<2>, so that the survival rate, the breeding rate and the seedling yield per unit area are greatly improved compared with the existing seedlings cultivation mode. The method is convenient to operate, can prevent the seedlings from being damaged caused by sand flushing and seedling driving, and is good for the long-distance transporting and the planting of the seedlings.

The invention provides a man health-care wine and a preparation method thereof. The health-care wine is prepared from the following components: cartialgenous, boschniakia rossica, cistanche, cynomorium songaricum, herba epimedii, hippocampi, testiset penis phocae, gecko, raw rehmannia, fructus corni, semen cuscutae, mulberry seeds, raspberry, schisandra chinensis, fruit of Chinese wolfberry, fructus cnidii, plantain seeds, lucid ganoderma, Jilin Ginseng, poria, cordyceps sinensis, rhodiola rosea, saussurea involucrate, deer penis, bullwhack, dog penis, bombyx mori, chilopod, angelica, radix paeoniae alba, radix glycyrrhizae preparata,black ant, red ant, fried Chinese date, prepared polygonum multiflorum thumb, white wine and yellow wine. The method comprises the following steps: taking white wine over 50 degrees, and pouring the wine until covering the medicinal materials, sealing, soaking for 36 days, stirring one time every three days; discarding the drug packaged by the gauze; adding the best-quality yellow wine the weight of which is two times of the white wine; adding a proper amount of honey according to the taste, sealing and soaking for 36 days, and stirring one time everythree days. The man health-care wine has the effects of tonifying kidney and cultivating yuan, strengthening du and softening blood vessel, replenishing qi and invigorating spleen, and nourishing blood and regulating liver after 50ml of prepared wine is taken every day.

The invention relates to a rapid detection kit and a detection method for bombyx mori nuclear polyhydrosis virus, specifically relates to a quick and convenient detection kit for detecting the bombyx mori nuclear polyhydrosis virus by combining the loop-mediated isothermal amplification technology and the nucleic acid detection lateral chromatography test paper, and a detection method of the detection kit, and belongs to the technical field of viral epidemic disease diagnosis. The detection kit is characterized by comprising a BmNPVLAMP pre-reaction liquid, wherein the desired primer and probe are designed according to the conserved region of the genomic sequence of the bombyx mori nuclear polyhydrosis virus published by GenBank; the kit is capable of effectively and quickly detecting the bombyx mori nuclear polyhydrosis virus; the steps of virus DNA extraction and BmNPVLAMP reaction system amplification are performed, and after the reaction ends, the result is judged by use of the quick nucleic acid detection test paper to obtain that the BmNPV virus DNA is efficiently and specifically amplified; the detection method is simple, convenient and quick, good in specificity, and high in sensitivity, and is suitable for farmers to use daily and advantageous for diagnosing and preventing the bombyx mori nuclear polyhydrosis virus disease.

The invention discloses a stiff silkworm culture method. The stiff silkworm culture method comprises the following steps of a. preparing a spore suspension: taking beauveria bassiana spores to prepare the spore suspension; b. infecting: feeding young silkworms; 12-24 hours after the young silkworms wake up from the fourth dormancy, spraying and inoculating the spore suspension obtained in the step a; feeding the young silkworms under the conditions that the temperature is 23-25 DEG C and the humidity is more than 95%; after 4 hours, repeatedly spraying and inoculating for one time; c. feeding the young silkworms which are infected in the step b for 48 hours under the conditions that the temperature is 23-25 DEG C and the humidity is more than 95%; feeding the young silkworms in a natural environment until the domestic silkworms grow up, harvesting and drying the silkworms. The stiff silkworm culture method disclosed by the invention can be used for culturing the stiff silkworms in a large scale and has high and stable yield; the quality of the cultured stiff silkworms is excellent and the industrial application prospect is good.