4 results about "Hydroxylase gene" patented technology

The disclosure related to water-miscible forms of astaxanthin produced by transgenic red algae strains transformed to express β-carotene ketolase and hydroxylase genes from a green algae. These water-miscible forms of astaxanthin are aggregations of astaxanthin secreted by the transformed red algae. The particles accumulate in the extracellular space and are enriched in stationary phase of the algae culture, which enables simple and efficient isolation and purification of astaxanthin from algae. Thus, also disclosed herein are methods of isolating and / or purifying astaxanthin from transgenic algae comprising separating algae cells from its culture solution to collect the supernatant and then centrifuging the supernatant to pellet astaxanthin for collection and optionally further purification.

This invention discloses a method for controlling the biotransformation of canlione by monitoring the expression level of hydroxylase gene. The steps are as follows: using *Aspergillus ochraceus* as the inoculum and canlione as the substrate, the expression level of the hydroxylase gene is monitored using real-time quantitative PCR during cell culture and transformation to determine the mid-logarithmic growth phase of the cell culture; canlione is then biotransformed again using *Aspergillus ochraceus* as the inoculum and canlione as the substrate, with an exogenous inducer added during the mid-logarithmic growth phase of the cell culture to improve the transformation rate. This method uses the hydroxylase gene expression level, a molecular biological indicator, as a key process parameter to guide the decision-making of substrate addition timing and the optimization of transformation conditions. It enables precise control of process optimization driven by gene expression data, achieving a significant increase in transformation rate and a significant reduction in transformation cycle, showing promising application prospects.

This invention relates to the field of biotechnology, and discloses an engineered *Yarrowia lipolytica* strain that produces p-coumaric acid using glucose as a substrate, its construction method, and its applications. Construction method: A tyrosine ammonia-lyase gene is integrated using a CRISPR / Cas9 localization and integration method. TAL In *Yarrowia lipophila* strains, the DAHP synthase gene was enhanced. ARO4 , DHS1 and AROG Overexpression of tyrosine synthase TYR Genes and histidine phosphotransferases HIS5 The protease gene was further integrated to synthesize exogenous phenylalanine deamination and hydroxylation pathway genes for p-coumaric acid, including phenylalanine ammonia-lyase gene, cinnamate hydroxylase gene, and P450 reductase gene. The p-coumaric acid produced by the engineered *Yarrowia lipolytica* strain of this invention can reach a maximum yield of 1.7 g / L in shake flasks, and a yield of 30 g / L in a 5 L fed-batch fermentation tank, demonstrating significant industrial application value.

The present application relates to a kind of fritillaria cirrhosa cholesterol 22 (R) -hydroxylase FcirCYP90B27 Gene and application, belong to the field of biotechnology.Fritillaria cirrhosa cholesterol 22 (R) -hydroxylase FcirCYP90B27 Gene nucleotide sequence as shown in SEQ ID NO.1, the amino acid sequence of encoding protein as shown in SEQ ID NO.2.The fritillaria cirrhosa cholesterol 22 (R) -hydroxylase FcirCYP90B27 Gene of the present application can be used as 22 (R) -hydroxylase biosynthesis control gene, and be applied to the preparation of 22 (R) -hydroxylase cholesterol, application prospect is remarkable, easy to popularization and application.