11 results about "Ligase Gene" patented technology

The invention belongs to the technical field of biology, and discloses a saccharomyces cerevisiae engineering strain for producing naringenin, a construction method and application, the saccharomyces cerevisiae engineering strain contains tyrosine ammonia lyase gene TAL, 4-cumaric acid: coenzyme A ligase gene 4CL, chalcone synthase gene CHS, chalcone isomerase gene CHI, oligopeptide gene RIADn-RGGm and oligopeptide gene RIDD; the ARO10 gene, the FDC1 gene, the PAD1 gene and the PDC5 gene are knocked out. The engineering strain disclosed by the invention can be used for synthesizing naringenin by taking L-tyrosine as a substrate, and the engineering strain is stable in hereditary character and excellent in naringenin yield which can reach 27.45 mg / L; and the fermentation production of the product can be carried out through relatively simple method conditions, so that the method has a very wide application prospect.

The application discloses application of a Pyrus betulaefolia E3 ubiquitin ligase gene PbrATL18 in genetic improvement of plant drought resistance and anthracnose resistance and belongs to the technical field of genetic engineering. The nucleotide sequence of the PbrATL18 gene is shown as SEQ ID NO. 1, and the amino acid sequence of the encoded protein is shown as SEQ ID NO. 2. The plant drought resistance and / or anthracnose resistance performance is improved by overexpressing the PbrATL18 gene in the plant or improving the protein activity. The application constructs a gene overexpression Arabidopsis transformation vector and a gene silencing P. betulaefolia seedling transformation vector respectively, obtains positive plant seedlings, and carries out drought and anthracnose treatment. The results show that overexpression of the E3 ubiquitin ligase gene PbrATL18 can effectively maintain the balance of active oxygen in cells, keep the cell osmotic potential stable, and significantly improve the plant drought resistance and anthracnose resistance performance, thereby providing a new idea for high drought tolerance and disease resistance breeding of pear trees.

The present application relates to the field of genetic engineering and bioengineering, and particularly relates to a genetically engineered surfactin-producing bacterium and application thereof.The genome of the genetically engineered bacterium comprises the following: an expression frame of T7 RNA polymerase; an expression frame of a surfactin synthesis gene cluster srfAABCD; and an expression frame of a long-chain fatty acid-coenzyme A ligase gene lcfA; the expression frame of the surfactin synthesis gene cluster srfAABCD and the expression frame of the long-chain fatty acid-coenzyme A ligase gene lcfA further comprise a T7 promoter.The present application realizes efficient synthesis of surfactin by Bacillus subtilis, and the final shake flask yield reaches more than 19.3 g / L, which has great industrial application potential.

The present invention introduces a heterologous formate-tetrahydrofolate ligase gene into a microorganism having a D-pantoic acid synthetic metabolic pathway, and assimilates one molecule of formate to generate one molecule of 5,10-methylenetetrahydrofolate, and uses the microorganism to produce a new process of ketopantoate and / or D-pantoic acid from formate and glucose.

The invention discloses a method for improving the self-luminous intensity of a plant based on energy optimization, which is characterized in that a lipoic acid protein ligase A gene LplA is expressed in a self-luminous plant containing a fungus bioluminescence system FBP, so that a transgenic plant of which the luminous intensity is remarkably improved is obtained. The LplA gene is introduced to optimize and modify an energy metabolism network and enhance the efficiency of energy regeneration in organisms, the strategy basically has no influence on the substrate content of a fungus bioluminescence system, and bioluminescence enhancement is finally realized by increasing the energy and cofactor content required in a fungus bioluminescence pathway. The energy bottleneck in a fungus bioluminescence system is broken through, and an innovative solution is provided for creating efficient self-luminous plants.

This invention provides a horse chestnut tiglyl-CoA ligase gene. AcCCL3 This invention relates to the AcCCL3 protein and its applications, belonging to the field of gene technology. For the first time, this invention has identified and verified the key enzyme gene responsible for the biosynthesis of tigrazol-CoA in horse chestnut. AcCCL3 This invention provides a complete analysis of its biosynthetic pathway, filling a knowledge gap in this field. It can be based on... AcCCL3 This gene, through synthetic biology strategies and an engineered system, has achieved the synthesis of tiglyl-CoA, completely eliminating reliance on traditional, complex, and demanding chemical synthesis routes. This significantly reduces production costs from the source, enabling large-scale production and successfully solving the core challenge of large-scale, stable preparation and supply of tiglyl-CoA. It also breaks through the key precursor constraints that have hindered the biosynthetic research, drug development, and industrial production of a series of high-value aescin compounds, represented by aescin A.

When employed, a recombinant expression vector carrying a daptomycin production gene including a foreign fatty acyl AMP ligase (FAAL) gene, a thioesterase (TE) gene, and a fatty acid synthase (FAS) gene according to the present invention can enhance specificity for decanoic acid, and produce daptomycin without supplementation of lipopeptides, such as decanoic acid, from the outside of the strain. In addition, no by-products are generated during the production process, thereby enabling production of daptomycin with high purity and high yield.

A wheat E3 ubiquitin ligase gene RING-finger E3 ligase 1 (RFEL1) and its application in wheat disease resistance. The nucleotide sequence of the wheat E3 ubiquitin ligase gene RFEL1 is as shown in SEQ ID NO. 3. The present disclosure further provides an application of the wheat E3 ubiquitin ligase gene RFEL1 or related biological materials thereof, including any one of the following applications: A1, enhancing wheat resistance to stripe rust; A2, enhancing wheat resistance to powdery mildew; and A3, enhancing wheat resistance to leaf rust.

The invention relates to the technical field of genetic engineering and bioengineering, in particular to a surfactin-producing genetic engineering bacterium and application thereof. A genome of the genetically engineered bacterium comprises the following components; an expression cassette encoding a T7 RNA polymerase; an expression cassette of the surfactin synthesis gene cluster srfAABCD; and an expression cassette of the long-chain fatty acid-coenzyme A ligase gene lcfA; the expression cassette of the surfactin synthetic gene cluster srfAABCD and the expression cassette of the long-chain fatty acid-coenzyme A ligase gene lcfA also comprise a T7 promoter. According to the method, the surfactin is efficiently synthesized by the bacillus subtilis, the final shake flask yield reaches 19.3 g / L or above, and the method has huge industrial application potential.

Provided is the use of a Citrus psyllid ubiquitin ligase UFD4 gene and an inhibitor thereof in the prevention and control of Citrus Huanglongbing. The amino acid sequence of a protein encoded by the Citrus psyllid ubiquitin ligase UFD4 gene is shown as SEQ ID NO. 1. By regulating the ubiquitin ligase UFD4 gene, the Citrus psyllid infection can be reduced, thereby effectively preventing and controlling the invasion and spread of Citrus Huanglongbing. The ubiquitin ligase UFD4 gene is also silenced by means of an E3-HECT type inhibitor, so as to study the ubiquitination function thereof during huanglongbing infection.

The invention belongs to the technical field of fungal gene engineering, and discloses an application of an E3 ubiquitin ligase gene LeDsc1 in regulation and control of the number of lentinula edodes (Lentinula edodes). By constructing a LeDsc1 gene overexpression and silence vector, a transgenic strain is obtained by using an agrobacterium-mediated genetic transformation method for gene function research. Results show that the LeDsc1 gene has a negative regulation effect on the number of the shiitake mushrooms, the number of the shiitake mushrooms can be remarkably increased by silencing the gene, the yield of the shiitake mushrooms is further effectively increased, and yield increase mainly originates from remarkable increase of the number of the shiitake mushrooms of the first tide.