25 results about "Ligase Gene" patented technology

The invention discloses a wheat E3 ubiquitin ligase gene RFEL1 and application of the wheat E3 ubiquitin ligase gene RFEL1 in wheat disease resistance, and belongs to the technical field of plant genetic engineering. The invention discloses a nucleotide sequence of a wheat E3 ubiquitin ligase gene RFEL1 as shown in SEQ ID NO. 3. The invention further provides application of the wheat E3 ubiquitin ligase gene RFEL1 or related biological materials thereof. The application comprises any one of the following applications: A1, improving the resistance of wheat to stripe rust; a2, the resistance of wheat to powdery mildew is improved; and A3, the resistance of wheat to leaf rust is improved. The E3 ubiquitin ligase RFEL1 gene provided by the invention not only has broad-spectrum resistance to different stripe rust, but also has resistance to wheat leaf rust and powdery mildew. The E3 ubiquitin ligase RFEL1 gene disclosed by the invention is applied to broad-spectrum resistance breeding of wheat and has important application value.

The invention discloses an E3 ubiquitin ligase gene ulA, the nucleotide sequence of which is shown as SEQ ID NO: 1. The gene is knocked out from fusarium oxysporum by using a homologous recombination method to obtain a mutant strain ulA; compared with a wild strain, the mutant hulA has the advantages that the spore yield is reduced, the spore germination is delayed, the response capability to various abiotic stresses is reduced, the pathogenicity to panax notoginseng plants is reduced, and the sensitivity to six bactericides is increased, and experimental results show that the gene plays an important role in pathogenicity, drug resistance and the like of fusarium oxysporum. The gene can be used as a target gene for developing an anti-fusarium oxysporum preparation.

The invention discloses a new application of human YTHDF1, namely an application of a human YTHDF1 gene expression quantity detection reagent in preparation of a prostatic cancer clinical diagnosis reagent. The expression of the human YTHDF1 gene in prostatic cancer tissues and cell lines is increased; after the expression of the YTHDF1 gene is knocked down, the growth of a prostate cancer cell line, the culture of organoids and the growth of transplanted tumors in nude mice are obviously inhibited; according to the present invention, with the application of the RNF7 in the tumor cells, the proliferation of the normal prostate epithelial cells can be promoted by the overexpression, the protein level of the RNF7 in the YTHDF1 knocked-down cells is significantly inhibited, the phenotype of the YTHDF1 knocked-down cells can be rescued by the overexpression of the RNF7, and the sensitivity of the tumor cells to the chemotherapeutic drugs can be promoted by the YTHDF1 knocked-down cells so as to promote the apoptosis; the prostatic cancer can be treated by inhibiting expression drugs of the human YTHDF1 gene, inhibiting expression of the E3 ubiquitin ligase RNF7 gene or screening drugs for degrading the E3 ubiquitin ligase RNF7.

The invention discloses an escherichia coli engineering strain with high benzyl acetate yield and application thereof. The escherichia coli strain efficiently expresses a brand new biosynthetic pathway related gene of benzyl acetate; comprising a phenylalanine lyase gene, a cinnamic acid coenzyme A ligase gene, a 3-hydroxyacyl coenzyme A dehydrogenase gene, a 3-oxoacyl coenzyme A ketohydrolase gene, an enoyl coenzyme A hydratase gene, a carboxylic acid reductase gene, a phosphoric acid pantetheinyl transferase gene and an acyltransferase gene. The obtained escherichia coli engineering strain can utilize a sustainable and cheap raw material glucose as a carbon source to realize efficient synthesis of benzyl acetate. The yield of benzyl acetate synthesized by the escherichia coli benzyl acetate engineering bacteria is the highest yield of benzyl acetate synthesized by a microbial fermentation method reported at home and abroad from beginning. The benzyl acetate produced by the engineering strain has the advantages of reliable quality, economic product, green production and the like, and has important economic value and social benefit.

The invention provides a genetically engineered bacterium, a preparation method of the genetically engineered bacterium and application of the genetically engineered bacterium in synthesis of resveratrol. The genetically engineered bacterium contains a p-coumaroyl coenzyme A ligase gene At4CL and a stilbene synthase gene PcSTS, wherein the p-coumaroyl coenzyme A ligase gene At4CL is derived from arabidopsis thaliana, and the stilbene synthase gene PcSTS is derived from Polygonum cuspidatum, and the p-coumaroyl coenzyme A ligase gene At4CL is derived from Polygonum cuspidatum. Wherein the genetically engineered bacteria are selected from escherichia coli. The method for catalytically synthesizing resveratrol by using the genetically engineered bacterium is short in fermentation period, high in yield and high in production intensity, has a relatively good application prospect, and lays a foundation for industrial production of resveratrol by using an escherichia coli microbial fermentation method.

The invention belongs to the technical field of biology, and discloses a saccharomyces cerevisiae engineering strain for producing naringenin, a construction method and application, the saccharomyces cerevisiae engineering strain contains tyrosine ammonia lyase gene TAL, 4-cumaric acid: coenzyme A ligase gene 4CL, chalcone synthase gene CHS, chalcone isomerase gene CHI, oligopeptide gene RIADn-RGGm and oligopeptide gene RIDD; the ARO10 gene, the FDC1 gene, the PAD1 gene and the PDC5 gene are knocked out. The engineering strain disclosed by the invention can be used for synthesizing naringenin by taking L-tyrosine as a substrate, and the engineering strain is stable in hereditary character and excellent in naringenin yield which can reach 27.45 mg / L; and the fermentation production of the product can be carried out through relatively simple method conditions, so that the method has a very wide application prospect.

The invention belongs to the technical field of plant heredity and genetic engineering, and particularly relates to a soybean E3 ubiquitin ligase gene GmSAL17 and application thereof. Specifically, an E3 ubiquitin ligase gene GmSAL17 is separated from soybean Williams 82, and the GmSAL17 gene is expressed in Arabidopsis thaliana and soybean hairy roots, so that the germination rate of transgenic Arabidopsis thaliana seeds under salt stress is higher than that of a control, and the salt tolerance of soybean hairy root overexpression transgenic plants is higher than that of the control plants. Meanwhile, three haplotypes of GmSAL17 in a natural soybean population are identified, and the three haplotypes are found to have significant difference in salt tolerance, which is mainly manifested by significant difference of fresh weight and root length of seedlings under salt stress. It is fully proved that the GmSAL17 gene plays an important role in plant salt tolerance regulation and control and can be used for plant salt tolerance regulation and control, and therefore the GmSAL17 gene has good practical application value.

The application discloses application of a Pyrus betulaefolia E3 ubiquitin ligase gene PbrATL18 in genetic improvement of plant drought resistance and anthracnose resistance and belongs to the technical field of genetic engineering. The nucleotide sequence of the PbrATL18 gene is shown as SEQ ID NO. 1, and the amino acid sequence of the encoded protein is shown as SEQ ID NO. 2. The plant drought resistance and / or anthracnose resistance performance is improved by overexpressing the PbrATL18 gene in the plant or improving the protein activity. The application constructs a gene overexpression Arabidopsis transformation vector and a gene silencing P. betulaefolia seedling transformation vector respectively, obtains positive plant seedlings, and carries out drought and anthracnose treatment. The results show that overexpression of the E3 ubiquitin ligase gene PbrATL18 can effectively maintain the balance of active oxygen in cells, keep the cell osmotic potential stable, and significantly improve the plant drought resistance and anthracnose resistance performance, thereby providing a new idea for high drought tolerance and disease resistance breeding of pear trees.

The invention belongs to the technical field of plant bioengineering, and particularly relates to a wheat grain weight related gene TaAPC11 and application of a KASP molecular marker of the wheat grain weight related gene TaAPC11. The KASP molecular marker is KASP-TaAPC11, and the marker detects an SNP (Single Nucleotide Polymorphism) site (C / A) at 364138181bp according to a 2B chromosome, namely, at the 845bp position of a 3'non-coding region of an E3 ubiquitin ligase gene TaAPC11. Association analysis finds that the genotype C / C is excellent allele variation for improving grain traits, and the grain weight and grain shape of TaAPC11 transgenic rice are increased. The functional KASP molecular marker of the wheat E3 ubiquitin ligase gene TaAPC11 provided by the invention can be used for identifying whether TaAPC11 excellent alleles exist in wheat varieties / strains or not, and can be applied to assisted selective breeding and pyramiding breeding with other known grain weight related genes.

The present application relates to the field of genetic engineering and bioengineering, and particularly relates to a genetically engineered surfactin-producing bacterium and application thereof.The genome of the genetically engineered bacterium comprises the following: an expression frame of T7 RNA polymerase; an expression frame of a surfactin synthesis gene cluster srfAABCD; and an expression frame of a long-chain fatty acid-coenzyme A ligase gene lcfA; the expression frame of the surfactin synthesis gene cluster srfAABCD and the expression frame of the long-chain fatty acid-coenzyme A ligase gene lcfA further comprise a T7 promoter.The present application realizes efficient synthesis of surfactin by Bacillus subtilis, and the final shake flask yield reaches more than 19.3 g / L, which has great industrial application potential.

The present invention discloses an E3 ubiquitin ligase gene SlRZFP34 , which is derived from tomato, and its nucleotide sequence is shown in SEQ ID NO:1. The expression level of this gene in tomato root tissue is higher than that in stems and leaves; its expression is induced by infection with Fusarium oxysporum; this gene is expressed in tobacco leaves, increasing tobacco's resistance to infection with Fusarium oxysporum; this gene has application potential in tomatoes to resist infection with Fusarium oxysporum.

The invention relates to the field of bioengineering, in particular to recombinant yarrowia lipolytica with high yield of denosine as well as a construction method and application of the recombinant yarrowia lipolytica. According to the invention, Yarrowia lipolytica is taken as a chassis cell, a phenylalanine ammonialyase gene PtrPAL3, a coumaroyl-coenzyme A ligase gene Pc4CL1 and a stilbene synthetase gene PtSTS are inserted into a genome, and 3-deoxy-D-arabinohepturonate-7-phosphate synthetase gene ARO3 and an acetyl-coenzyme A carboxylase gene ACC1 are overexpressed, so that the Yarrowia lipolytica is obtained. The 4-hydroxyphenylpyruvate dioxygenase gene HPD and the phenylpyruvate decarboxylase gene ARO10 are knocked out, and the recombinant yarrowia lipolytica is constructed and obtained. After genetic modification, the strain has the capability of synthesizing the pinosine from the beginning, the fermentation yield reaches 260mg / L by using a glucose shake flask, and the yield of the pinosine fermented in a 5L fermentation tank reaches 1200mg / L. According to the method, high-efficiency production of the pinosine is realized by utilizing the yarrowia lipolytica for the first time, and the pinosine yield of the constructed recombinant yarrowia lipolytica is the highest level of microbial fermentation production at present.

This invention belongs to the field of genetic engineering, and specifically relates to a 4-coumaric acid-coenzyme A ligase gene Gl4CL from *Gynostemma pentaphyllum*, its encoded product, and its applications. This invention is the first to clone and prepare the 4-coumaric acid-coenzyme A ligase gene Gl4CL from the medicinal plant *Gynostemma pentaphyllum*. The nucleotide sequence of this gene is shown in SEQ ID NO.1, and the amino acid sequence of the product encoded by this nucleotide sequence is shown in SEQ ID NO.2. The 4-coumaric acid-coenzyme A ligase gene Gl4CL is a key regulatory gene in the flavonoid biosynthesis pathway of *Gynostemma pentaphyllum*. This enzyme can be used in the preparation of p-coumaryl-CoA using p-coumaric acid as a substrate, further promoting the biosynthesis of flavonoid compounds. This invention can provide an effective method to meet the huge market demand for flavonoid compounds.

The present invention introduces a heterologous formate-tetrahydrofolate ligase gene into a microorganism having a D-pantoic acid synthetic metabolic pathway, and assimilates one molecule of formate to generate one molecule of 5,10-methylenetetrahydrofolate, and uses the microorganism to produce a new process of ketopantoate and / or D-pantoic acid from formate and glucose.

Task of the present disclosure is to provide a solanaceous plant and cells thereof and a method for producing the solanaceous plant where the plant has inhibitory properties against infection by a virus of genus Begomovirus causing tomato yellow leaf curl symptoms, propagation of the infected virus, and / or expression of infection symptoms, and the plant is resistant to the virus. The task is solved by providing a solanaceous plant having a mutation in at least one gene selected from a group consisting of DCL3 gene which is silencing associated dicer gene, 4CL06 gene which is 4-coumarate-CoA ligase gene, RLK2 gene which is receptor-like kinase gene, and genes homologous thereto. The mutation either inhibits the expression of the mutated gene or makes a protein encoded by the mutated gene to be non-functional for the virus, and the plant has virus resistance against the above-mentioned virus.

The invention discloses a method for improving the self-luminous intensity of a plant based on energy optimization, which is characterized in that a lipoic acid protein ligase A gene LplA is expressed in a self-luminous plant containing a fungus bioluminescence system FBP, so that a transgenic plant of which the luminous intensity is remarkably improved is obtained. The LplA gene is introduced to optimize and modify an energy metabolism network and enhance the efficiency of energy regeneration in organisms, the strategy basically has no influence on the substrate content of a fungus bioluminescence system, and bioluminescence enhancement is finally realized by increasing the energy and cofactor content required in a fungus bioluminescence pathway. The energy bottleneck in a fungus bioluminescence system is broken through, and an innovative solution is provided for creating efficient self-luminous plants.

This invention provides a horse chestnut tiglyl-CoA ligase gene. AcCCL3 This invention relates to the AcCCL3 protein and its applications, belonging to the field of gene technology. For the first time, this invention has identified and verified the key enzyme gene responsible for the biosynthesis of tigrazol-CoA in horse chestnut. AcCCL3 This invention provides a complete analysis of its biosynthetic pathway, filling a knowledge gap in this field. It can be based on... AcCCL3 This gene, through synthetic biology strategies and an engineered system, has achieved the synthesis of tiglyl-CoA, completely eliminating reliance on traditional, complex, and demanding chemical synthesis routes. This significantly reduces production costs from the source, enabling large-scale production and successfully solving the core challenge of large-scale, stable preparation and supply of tiglyl-CoA. It also breaks through the key precursor constraints that have hindered the biosynthetic research, drug development, and industrial production of a series of high-value aescin compounds, represented by aescin A.

When employed, a recombinant expression vector carrying a daptomycin production gene including a foreign fatty acyl AMP ligase (FAAL) gene, a thioesterase (TE) gene, and a fatty acid synthase (FAS) gene according to the present invention can enhance specificity for decanoic acid, and produce daptomycin without supplementation of lipopeptides, such as decanoic acid, from the outside of the strain. In addition, no by-products are generated during the production process, thereby enabling production of daptomycin with high purity and high yield.

A wheat E3 ubiquitin ligase gene RING-finger E3 ligase 1 (RFEL1) and its application in wheat disease resistance. The nucleotide sequence of the wheat E3 ubiquitin ligase gene RFEL1 is as shown in SEQ ID NO. 3. The present disclosure further provides an application of the wheat E3 ubiquitin ligase gene RFEL1 or related biological materials thereof, including any one of the following applications: A1, enhancing wheat resistance to stripe rust; A2, enhancing wheat resistance to powdery mildew; and A3, enhancing wheat resistance to leaf rust.

The application discloses a wheat E3 ubiquitin ligase gene RFEL1 and application thereof in wheat disease resistance, and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the wheat E3 ubiquitin ligase gene RFEL1 is shown as SEQ ID NO. 3. The application also provides application of the wheat E3 ubiquitin ligase gene RFEL1 or related biological materials thereof, including application of any one of the following: A1, improving the resistance of wheat to stripe rust; A2, improving the resistance of wheat to powdery mildew; and A3, improving the resistance of wheat to leaf rust. The E3 ubiquitin ligase RFEL1 gene provided by the application not only has broad-spectrum resistance to different stripe rusts, but also has resistance to wheat leaf rust and powdery mildew. The E3 ubiquitin ligase RFEL1 gene of the application has important application value when used in wheat broad-spectrum resistance breeding.

The invention belongs to the field of gene engineering, and particularly relates to a glechoma longituba 4-coumaric acid-coenzyme A ligase gene Gl4CL as well as an encoded product and application thereof. The glechoma longituba 4-coumaric acid coenzyme A ligase gene Gl4CL is cloned, prepared and obtained from glechoma longituba medicinal plants for the first time, the nucleotide sequence of the gene is shown as SEQ ID NO.1, and the amino acid sequence of a product coded by the nucleotide sequence is shown as SEQ ID NO.2. The glechoma longituba 4-coumaric acid coenzyme A ligase gene Gl4CL is a key regulatory gene in a glechoma longituba flavonoid biosynthetic pathway, and the ligase can be applied to the process of preparing p-coumaryl coenzyme A by taking p-coumaric acid as a substrate, so that the biosynthesis of a flavonoid compound is further promoted. The invention can provide an effective method for meeting the huge market demand faced by the flavonoid compound.

The invention relates to the technical field of genetic engineering and bioengineering, in particular to a surfactin-producing genetic engineering bacterium and application thereof. A genome of the genetically engineered bacterium comprises the following components; an expression cassette encoding a T7 RNA polymerase; an expression cassette of the surfactin synthesis gene cluster srfAABCD; and an expression cassette of the long-chain fatty acid-coenzyme A ligase gene lcfA; the expression cassette of the surfactin synthetic gene cluster srfAABCD and the expression cassette of the long-chain fatty acid-coenzyme A ligase gene lcfA also comprise a T7 promoter. According to the method, the surfactin is efficiently synthesized by the bacillus subtilis, the final shake flask yield reaches 19.3 g / L or above, and the method has huge industrial application potential.

Provided is the use of a Citrus psyllid ubiquitin ligase UFD4 gene and an inhibitor thereof in the prevention and control of Citrus Huanglongbing. The amino acid sequence of a protein encoded by the Citrus psyllid ubiquitin ligase UFD4 gene is shown as SEQ ID NO. 1. By regulating the ubiquitin ligase UFD4 gene, the Citrus psyllid infection can be reduced, thereby effectively preventing and controlling the invasion and spread of Citrus Huanglongbing. The ubiquitin ligase UFD4 gene is also silenced by means of an E3-HECT type inhibitor, so as to study the ubiquitination function thereof during huanglongbing infection.

The invention belongs to the technical field of fungal gene engineering, and discloses an application of an E3 ubiquitin ligase gene LeDsc1 in regulation and control of the number of lentinula edodes (Lentinula edodes). By constructing a LeDsc1 gene overexpression and silence vector, a transgenic strain is obtained by using an agrobacterium-mediated genetic transformation method for gene function research. Results show that the LeDsc1 gene has a negative regulation effect on the number of the shiitake mushrooms, the number of the shiitake mushrooms can be remarkably increased by silencing the gene, the yield of the shiitake mushrooms is further effectively increased, and yield increase mainly originates from remarkable increase of the number of the shiitake mushrooms of the first tide.

The present invention relates to a microorganism and a method for the fermentative production of guanidinoacetic acid (GAA) using such a microorganism, the present invention relates to a microorganism comprising at least one heterologous gene encoding L-arginine: glycine amidino transferase (AGAT) and at least one heterologous L-threonine 3-dehydrogenase gene (tdh) and at least one heterologous glycine C-acetyltransferase (2-amino-3-oxobutyrate CoA ligase) gene (kbl). The invention also relates to a method for the fermentative production of creatine.