182 results about "Caffeic acid" patented technology

The invention provides a metal polyphenol nanoparticle, a preparation method thereof and an application of the metal polyphenol nanoparticle in preparation of a medicine for treating acute lung injury, the metal polyphenol nanoparticle is formed by self-assembly of metal ions and polyphenol, the metal ions are selected from Mg < 2 + >, Zn < 2 + >, Mn < 2 + > or Cu < 2 + >, and the polyphenol is selected from Mg < 2 + >, Zn < 2 + >, Mn < 2 + > or Cu < 2 + >. The polyphenol is selected from epigallocatechin gallate (EGCG), caffeic acid (CA), chlorogenic acid (CGA), gallic acid (GA) and luteolin (LUT). The metal polyphenol nanoparticles are used for preparing a medicine for treating acute lung injury induced by sepsis, pneumonia, serious trauma or inhalation injury, and the medicine further comprises a pharmaceutically acceptable carrier. The metal polyphenol nanoparticles provided by the invention are simple in preparation process and high in biological safety, polyphenol and metal ions synergistically enhance the anti-inflammatory and antioxidant activity, and the metal polyphenol nanoparticles are superior to the single use of polyphenol or metal ions.

Disclosed are modified Type 1 O-methyltransferases (OMTs) and methods of using such modified OMTs. In some forms of the modified OMTs, the amino acid corresponding to Phe337 of Sorghum bicolor stilbene OMT (SbSOMT) is substituted with a polar amino acid. In some forms of the modified OMTs, the amino acid corresponding to Asn323 of Sorghum bicolor caffeic acid OMT (SbCOMT) is substituted with a hydrophobic amino acid. In some forms of the modified OMTs, the amino acid corresponding to Ile144 of SbSOMT is substituted with a polar amino acid. In some forms of the modified OMTs, the amino acid corresponding to Asn128 of SbCOMT is substituted with a hydrophobic amino acid.

The invention discloses a nano-drug material based on self-assembly of berberine and caffeic acid and a preparation method of the nano-drug material. The preparation method comprises the following steps: respectively dissolving berberine and caffeic acid in an organic solvent to prepare a berberine-organic solvent solution and a caffeic acid-organic solvent solution; adjusting the pH value of the caffeic acid-organic solvent solution to 7.0-7.5; adding the caffeic acid-organic solvent solution while stirring the berberine-organic solvent solution, uniformly mixing, adding the mixed solution into deionized water with the preheating temperature of 30-70 DEG C, and stirring at a constant temperature, so that the berberine and the caffeic acid are self-assembled to obtain a mixed solution; dialyzing the mixed solution to obtain a berberine and caffeic acid self-assembled solution; and performing vacuum freeze drying to obtain a target product. The nano-drug material disclosed by the invention is small in particle size, better in solubility and dissolution rate in different pH environments than berberine hydrochloride and caffeic acid eutectic, good in stability in an aqueous solution and environment-friendly in method.

The invention relates to a preparation method of a mixture of sorghum starch and caffeic acid. The preparation method is characterized by comprising the following steps: mixing water, sorghum starch and caffeic acid to prepare a sorghum starch-caffeic acid mixed solution; then carrying out microblog treatment on the sorghum starch-caffeic acid mixed solution; after the treatment is finished, cleaning with an ethanol solution to remove free caffeic acid components in a reaction system, and centrifuging to take white precipitate at the lower layer; and freeze-drying to obtain a mixture of sorghum starch and caffeic acid. The SS-CA compound provided by the invention has a more stable gel continuous phase in the aging process. At the same aging time point, the moisture distribution of the SS-CA composite system has a lower free water peak area proportion, and the moisture retention capability is improved.

The invention discloses a method for improving the self-luminous intensity of a plant, which is characterized in that an AroGL175Q gene or an RgTAL gene and a PtC3H gene or the AroGL175Q gene, the RgTAL gene and the PtC3H gene are expressed in a plant containing a fungus bioluminescence system FBP, so that a transgenic plant of which the caffeic acid content, the milk tree alkali content and the luminous intensity are obviously increased is obtained. According to the invention, through metabolic network optimization and transformation, phenylpropane metabolites required by middle and upper streams of a fungus bioluminescence pathway are increased, and synthesis of caffeic acid and milk tree alkali in plants is increased, so that bioluminescence enhancement is realized. The precursor supply bottleneck of a fungus bioluminescence system is broken through, and an innovative solution is provided for creating efficient self-luminous plants.

Methods, compositions and uses for inhibiting the growth in blood cancer cells in a patient with one or more of a caffeic acid phenpropyl ester (GL8) analogue selected from the group consisting of As26, J229, J91, LL27, LL23, HM7, As25, MT26, and J205. The blood cancer cells can be myeloma, lymphoma and leukemia cells. The methods, compositions and uses can be in conjunction with the use of an IMiD to treat a patient. The compositions can include a pharmaceutically acceptable carrier, adjuvant or vehicle, a pharmaceutically acceptable salt or dietary supplement.

The invention discloses an application of caffeic acid-O-methyltransferase in regulation and control of salt tolerance of rice. The overexpressed OsCOMT gene disclosed by the invention can remarkably improve the melatonin content and antioxidant level of rice seedlings, so that the tolerance of plants to salt stress is enhanced, and the plants can maintain normal growth under the condition of salt stress; the deletion of the gene OsCOMT leads to the increase of the sensitivity of seedling-stage rice to salt stress, and exogenous melatonin can recover the salt-sensitive phenotype of a mutant-knocked-out plant and improve the photosynthetic rate of the mutant-knocked-out plant. The OsCOMT gene and the encoding protein thereof have important theoretical and practical significance on research of a plant stress tolerance mechanism and improvement of stress tolerance and related traits of plants, can play an important role in plant stress tolerance gene engineering improvement, and are wide in application prospect.

The invention discloses a pH response type multifunctional hydrogel dressing and a preparation method and application thereof, and belongs to the field of medical hydrogel dressings, the pH response type antibacterial hydrogel provided by the invention comprises the following components: a gel matrix, a cross-linking agent and a natural antibacterial active component; firstly, a bond is formed through a Schiff base reaction, so that the gel can be responded and released in a wound acid environment; and then connecting natural active antibacterial drugs such as caffeic acid, tannic acid or luteolin through boric acid ester bonds to obtain the pH-responsive antibacterial hydrogel. The pH-responsive antibacterial hydrogel prepared by the invention has good absorption capacity, a porous structure, adjustable mechanical strength and biocompatibility, also has good antibacterial, anti-inflammatory and anti-oxidation effects, provides a simple and effective method for repairing chronic wounds, and has good application prospects.

The invention provides a fingerprint spectrum and multi-component content determination method of a calamus-far nerve calming mixture, and relates to the field of quality control of traditional Chinese medicines. 31 common peaks are calibrated by establishing a fingerprint spectrum, and 24 common peaks can clearly belong to polygala tenuifolia. Meanwhile, synchronous content determination of seven components including chlorogenic acid, caffeic acid, ferulic acid, liquiritin, 2, 3, 5, 4 '-tetrahydroxy stilbene-2-O-beta-D-glucoside, 3, 6'-dimustard acyl sucrose and rosmarinic acid is realized. The method is good in precision, high in repeatability and stable and reliable in result, the medicine quality can be comprehensively and accurately reflected and controlled, and powerful support is provided for guaranteeing the safety and effectiveness of the calamus-far Ningmind mixture.

The invention discloses a recombinant bacterium for efficiently synthesizing caffeic acid as well as a construction method and application of the recombinant bacterium, and relates to the technical field of genetic engineering. It is found that fre, mdaB, hisP and gltL genes in tyrosine producing bacteria are specifically reduced or knocked out, then caffeic acid synthetic pathway enzyme expression vectors are transferred, the production capacity of caffeic acid of the strains can be remarkably improved, the production capacity can be improved by 11.5%-20.4% on the original basis, and when the tyrosine producing bacteria are applied to caffeic acid production, the yield of caffeic acid can be remarkably improved.

The invention discloses recombinant saccharomyces cerevisiae, a 11-seed oil fermentation product as well as a preparation method and application of the 11-seed oil fermentation product, and belongs to the technical field of synthetic biology and fermentation product preparation. According to the recombinant saccharomyces cerevisiae disclosed by the invention, a caffeic acid coenzyme A ligase 4CL coding gene, a chalcone isomerase CHI coding gene, a flavanone-3-hydroxylase F3H coding gene and a chalcone synthase CHS mutant coding gene are over-expressed, and the chalcone synthase CHS mutant is preferably Y69H / H71Y / Q161K. The preparation method of the 11-seed oil fermentation product comprises the following steps: fermenting a recombinant saccharomyces cerevisiae fermentation solution in the presence of a sophora flower bud extract and caffeic acid to obtain a first fermentation solution; fermenting the lactic acid bacteria fermentation liquor in the presence of 11 seed oil to obtain second fermentation liquor; and mixing the first fermentation liquid and the second fermentation liquid, continuing fermentation, and respectively collecting an oil phase and a water phase after fermentation is finished, so as to obtain a fermentation product. The product provided by the invention has multivitamin antioxidant activity.

The invention relates to the field of wastewater treatment, in particular to a water quality conditioner added with improved humic acid and a preparation method of the water quality conditioner. The water quality conditioner added with the improved humic acid is obtained by compounding the improved humic acid and interface functionalized hematite nanocrystals; the improved humic acid is methacrylic acid grafted phenol modified humic acid; the interface functionalized hematite nanocrystals are selected from one of caffeic acid interface functionalized hematite nanocrystals and benzimidazole interface functionalized hematite nanocrystals. No toxic reducing agent or precious metal is used; caffeic acid and benzimidazole are degradable or low-toxicity small molecules, and the residual quantity is low; the final product is Cr (III) hydroxide or organic complex, can be further precipitated or recycled, and accords with the concept of green water treatment. All reaction preparation conditions are mild, and equipment requirements are low. The raw materials are wide in source, low in cost and suitable for industrial production.

The invention belongs to the technical field of organic synthesis and cosmetics, and particularly relates to a synthesis method of caffeoyl tripeptide-1. According to the invention, caffeic acid and tripeptide-1 are used as raw materials, and a condensation reaction is carried out under mild conditions through a specific activation strategy and a protective group technology to generate a target product. The method has the advantages of simple steps, mild reaction conditions, high total yield, good product purity and the like.

The present invention relates to a nanoparticle system comprising a metal selected from silver and gold, coated with polyethylene glycol (PEG) and functionalised with a polyphenol organic acid selected from caffeic acid, gallic acid and ferulic acid. The present invention also relates to a method for manufacturing said nanoparticle system and to the therapeutic and cosmetic use of said nanoparticle system.

The present application relates to the technical field of medicine, and particularly relates to a drug composition with a sequential synergistic repair effect and a preparation method and application thereof. The drug composition comprises liposome I encapsulating caffeic acid and liposome II encapsulating BH4; the liposome I is a ROS-responsive liposome, can specifically respond in a high ROS microenvironment, and then realize on-demand release of caffeic acid; meanwhile, the liposome II protects BH4 from entering cells to play a role, can effectively overcome the defects of difficult dissolution of caffeic acid and easy oxidation of caffeic acid and BH4, can improve the bioavailability of the two drugs, can realize sequential release of caffeic acid and BH4, and can play a synergistic treatment effect on different mechanisms of occurrence of radioactive skin damage, and provides a sequential synergistic repair strategy for clinic. The liposome I and the liposome II are both prepared by a thin film hydration method, and the operation is simple, the conditions are mild, the lipid film is uniformly formed, and the encapsulation rates of caffeic acid and BH4 are high.

The invention discloses polymer-based anti-crack concrete and a preparation method thereof, and relates to the technical field of concrete. When the polymer-based anti-crack concrete is prepared, caffeic acid and glycidyl methacrylate react to prepare a chain extender; the preparation method comprises the following steps: reacting isophorone diisocyanate with polyethylene glycol and a chain extender in sequence to prepare polyurethane, and then reacting with mercaptopropyltrimethoxysilane to prepare modified polyurethane; the preparation method comprises the following steps: reacting carboxylated cellulose nanofibers with 4-amino-2, 2, 6, 6-tetramethylpiperidine to prepare pre-modified nanocellulose, and reacting the pre-modified nanocellulose with isocyanate propyl triethoxy silane to prepare modified nanocellulose; uniformly mixing the fine aggregate, the coarse aggregate, cement, fly ash, the modified polyurethane, the modified nano cellulose, water, a water reducing agent and a defoaming agent, pouring into a mold, vibrating, standing, demolding and curing to obtain the polymer-based anti-crack concrete. The polymer-based anti-crack concrete prepared by the preparation method disclosed by the invention has good anti-crack and anti-aging properties.

The present application relates to a method for introducing an exogenous caffeic acid synthesis pathway to improve the light intensity of plants, and introducing TAL genes and P450 genes into plants to obtain a significant up-regulation effect of caffeic acid expression amount. This may be because the introduction of TAL genes and P450 genes promotes the deamination of L-DOPA, increases the accumulation amount of L-DOPA, and further effectively increases the caffeic acid expression amount in plants. Based on this pathway, the synthesis of luciferin in plants is increased to enhance the light intensity of plants.

The invention belongs to the technical field of traditional Chinese medicine detection, and particularly relates to a construction method of a specific chromatogram of caulis clematidis armandii or derivative products thereof. The invention provides a construction method of a characteristic chromatogram of caulis clematidis armandii or a derivative product thereof, which comprises the following steps: by taking octadecylsilane chemically bonded silica as a filler and a mobile phase comprising a formic acid-containing aqueous solution and acetonitrile, carrying out a specific gradient elution procedure to obtain 22 common characteristic peaks, and realizing effective separation of the common characteristic peaks, so that the separation degree is good, and the accuracy is high. The obtained characteristic spectrum has more characteristic peaks and better baseline separation, and is easier to position and qualitatively analyze; the precision, the stability and the repeatability are relatively good; compared with the prior art, the method has the advantages that the characteristic peaks are more, six characteristic components including the straight clematis seed B, the lariciresinol, the secoisolariciresinol, the lariciresinol-4-O-beta-D-glucoside, the caffeic acid and the ferulic acid are identified, and a basis is provided for quality detection and content determination of the caulis clematidis armandii or derivative products of the caulis clematidis armandii.

A method for preparing silkworm pupa active peptide microcapsules and their applications are disclosed. In a microfluidic focusing droplet generation chip, an aqueous phase is injected into the chip through the main channel, and an oil phase is injected through two side channels. The resulting microdroplets are collected in the collecting phase to form microcapsules. The filter membrane has a pore size of 0.1-1 μm. The aqueous phase consists of sodium alginate and silkworm pupa protein peptides; the oil phase consists of one of mineral oil, soybean oil, silkworm pupa oil, corn oil, peanut oil, rapeseed oil, and lard; the collecting phase consists of caffeic acid-grafted chitosan and calcium chloride dissolved in acetic acid. By optimizing the formulation and process, droplets with good dispersibility in oil, uniform morphology and size, and good stability are prepared.

The present invention relates to tablet, capsule and ampoule formulations for oral and / or injectable use, said formulations comprising beta-D-mannuronic acid or alpha-L-guluronic acid or a mixture of beta-D-mannuronic acid and alpha-L-guluronic acid, their oligomers, or their pharmaceutically acceptable salts, the total amount of [beta]-D-mannuronic acid or [alpha]-L-guluronic acid or mixtures thereof, oligomers thereof, or pharmaceutically acceptable salts thereof is from 10% to 99.999% by weight, based on the total weight of the tablet or capsule formulation, or from 20% to 60% by weight, based on the total weight of the ampoule formulation; the tablet, capsule or ampoule formulation further comprises caffeic acid or a salt thereof, for example in powder form, in an amount preferably from 0.001 wt% to 1 wt%, more preferably from 0.01 wt% to 0.5 wt%, more preferably from 0.05 wt% to 0.3 wt%, based on the total weight of the tablet, capsule or ampoule formulation. Furthermore, the present invention relates to the oral and / or injectable use of tablet, capsule and ampoule formulations, preferably as supplements or medicaments, for use in methods for the treatment of neurodegenerative diseases, inflammatory responses, pain, cancer, aging, diabetes and / or heart diseases, as well as to methods of preparing such formulations.

This invention relates to the field of adhesive technology, and discloses an environmentally friendly biomass-based underwater adhesive, its preparation method, and its applications. The adhesive is prepared by one-step melt copolymerization of three biomass raw materials: lipoic acid, caffeic acid, and cysteine. This system utilizes the ring-opening polymerization of lipoic acid to form a dynamic disulfide bond network providing cohesive force, the catechol groups of caffeic acid to provide various interfacial interactions with the substrate, and the thiol groups of cysteine ​​to simultaneously participate in network construction and inhibit catechol oxidation, thereby synergistically achieving high-strength and durable underwater adhesion. The adhesive achieves an underwater adhesion strength of up to 5.5 MPa on iron sheets, remains stable in various acid, alkali, and salt environments, and can be repeatedly recycled through hot melting. This invention uses entirely bio-based raw materials, employs a simple and non-toxic process, and is suitable for underwater remediation, marine engineering, and other fields.

The invention belongs to the technical field of medicines, and particularly relates to application of an echinacea caffeic acid derivative to preparation of a medicine or a feed additive for treating animal periodontal diseases and a composition containing the echinacea caffeic acid derivative. The invention discloses application of an echinacea caffeic acid derivative in preparation of a medicine for treating animal periodontal diseases. Experiments prove that the echinacea caffeic acid derivative has the effects of improving feline gingivitis, gingival bleeding, gingival atrophy, alveolar bone loss and the like, can be used for treating and preventing animal periodontal diseases, and has smaller toxic and side effects while ensuring that a better periodontal disease treatment effect can be realized.

The invention discloses ROS (reactive oxygen species) responsive hydrogel loaded with hypoxia-induced exosome as well as a preparation method and application of the ROS responsive hydrogel. The ROS responsive hydrogel comprises an ROS responsive hydrogel matrix and a hypoxia-induced exosome dispersed in the ROS responsive hydrogel matrix, and the ROS responsive hydrogel matrix is prepared by uniformly mixing hyaluronic acid grafted with 3-aminophenylboronic acid and epsilon-polylysine grafted with caffeic acid. Under a high ROS environment, boric acid ester bonds in the hydrogel can be destroyed to release exosomes and caffeic acid, and the exosomes induced by hypoxia contain more factors for promoting tissue repair and regeneration and also have the competitive advantage of high uptake rate. The ROS responsive hydrogel loaded with the hypoxia-induced exosome can realize on-demand release of the exosome, delivers the exosome with endogenous competitive advantages to an ischemic infarction region and removes excessive active oxygen to play a role in efficiently repairing myocardial infarction.

The invention discloses a modified electrode, a preparation method of the modified electrode and application of the modified electrode in caffeic acid detection, and relates to the technical field of electrochemical detection.The preparation method of the electrode comprises the following steps that firstly, an Au nano solution is prepared; 2, preparing a polyaniline hollow nanotube suspension liquid; 3, pretreating the glassy carbon electrode; and 4, modifying the glassy carbon electrode by using gold nanoparticle-polyaniline. The modified electrode has the advantages of being simple in preparation process, high in detection stability and high in detection sensitivity, the application prospect is wide, and good research, development and application values are achieved.

The invention provides application of a phellinus polyketide synthase gene SvPKS1 in promoting bioluminescence of fungi or plants, the coding sequence of the SvPKS1 is shown as SEQ ID NO.1, and the fungi are fungi for producing caffeic acid. According to the application, the SvPKS1 and other genes (nnH3H, nnLuz and nnCPH) of an FBP fungus luminescence pathway are co-expressed in fungi or plants by utilizing a transgenic technology, so that the fungi or plants are promoted to emit light biologically. The invention discloses co-expression of the phellinus polyketide synthase gene SvPKS1 and fungus luminescence pathway genes nnH3H, nnLuz and nnCPH for the first time, promotes fungus or plant bioluminescence, and provides a key candidate enzyme gene for promoting fungus or plant sustainable luminescence.

The present invention provides a hair-coating composition that comprises: a first polyphenol containing at least two of tannic acid, gallic acid, and caffeic acid; and a dye, and thus can restore hair and improve mechanical strength through a Michael addition reaction with thiol groups contained in damaged hair, wherein hair-coating layers formed by the composition prevent separation of the dye and thus maintain dyeability of the dye.

The invention belongs to the technical field of molecular plant breeding, and particularly relates to a poplar drought stress resistance gene and application thereof, and the nucleotide sequence of the gene is shown as SEQ ID NO: 1. After the gene is over-expressed in the poplar, the drought resistance of the plant can be obviously improved, and the growth and development of the plant cannot be inhibited; according to the present invention, the Caffeic acid O-methylated transferase 2-direct evolution gene is the drought stress resistance key gene, such that the gene can be used for culturing the high-yield, high-quality and drought resistance new variety, has the great application value on the forest comprehensive stress resistance genetic improvement, and provides the new molecular tool for the forest drought resistance genetic improvement.

The invention provides a method for increasing the synthesis amount of lignin in arabidopsis thaliana by using an agropyron cristatum AmCOMT gene, and belongs to the field of plant genetic engineering. The method comprises the following steps: transferring a nucleic acid molecule which is derived from Mongolian agropyron cristatum and is used for coding caffeic acid oxygen methyl transferase into arabidopsis thaliana, and enabling the arabidopsis thaliana to over-express the caffeic acid oxygen methyl transferase. According to the application, the COMT gene of the agropyron mongolicum is successfully transferred into the arabidopsis thaliana and is efficiently expressed in the arabidopsis thaliana, so that the transgenic arabidopsis thaliana containing the AmCOMT gene is obtained, the content of lignin in the cell wall of the arabidopsis thaliana is remarkably increased, and the stress resistance of the arabidopsis thaliana to drought, high-salt and other environments is remarkably enhanced.

The application discloses a kind of Shenkun Yangxue granules fingerprint and its construction method, belong to traditional Chinese medicine detection technical field.Different batches of Shenkun Yangxue granules are prepared into test sample solution;Respectively with Danshensu, ephedrine hydrochloride, syringin, protocatechuic aldehyde, coffee acid, calycosin-7-O-β-D-glucoside, loganin, comphoric acid, shisonin, delphinidin and luteolin and kaempferol control preparation single control solution;Test sample solution and control solution are analyzed by high performance liquid chromatography and high resolution mass spectrometry respectively, 18 characteristic peaks are screened out, and 12 kinds of chemical components are identified by standard comparison and fragment information analysis, to obtain Shenkun Yangxue granules fingerprint.The Shenkun Yangxue granules fingerprint established by the method can be used for the quality control detection of Shenkun Yangxue granules, which is conducive to the comprehensive monitoring of product quality, and ensures the consistency, controllability and stability of product quality.