141 results about "Caffeic acid" patented technology

The invention discloses a nano-drug material based on self-assembly of berberine and caffeic acid and a preparation method of the nano-drug material. The preparation method comprises the following steps: respectively dissolving berberine and caffeic acid in an organic solvent to prepare a berberine-organic solvent solution and a caffeic acid-organic solvent solution; adjusting the pH value of the caffeic acid-organic solvent solution to 7.0-7.5; adding the caffeic acid-organic solvent solution while stirring the berberine-organic solvent solution, uniformly mixing, adding the mixed solution into deionized water with the preheating temperature of 30-70 DEG C, and stirring at a constant temperature, so that the berberine and the caffeic acid are self-assembled to obtain a mixed solution; dialyzing the mixed solution to obtain a berberine and caffeic acid self-assembled solution; and performing vacuum freeze drying to obtain a target product. The nano-drug material disclosed by the invention is small in particle size, better in solubility and dissolution rate in different pH environments than berberine hydrochloride and caffeic acid eutectic, good in stability in an aqueous solution and environment-friendly in method.

The invention discloses a method for improving the self-luminous intensity of a plant, which is characterized in that an AroGL175Q gene or an RgTAL gene and a PtC3H gene or the AroGL175Q gene, the RgTAL gene and the PtC3H gene are expressed in a plant containing a fungus bioluminescence system FBP, so that a transgenic plant of which the caffeic acid content, the milk tree alkali content and the luminous intensity are obviously increased is obtained. According to the invention, through metabolic network optimization and transformation, phenylpropane metabolites required by middle and upper streams of a fungus bioluminescence pathway are increased, and synthesis of caffeic acid and milk tree alkali in plants is increased, so that bioluminescence enhancement is realized. The precursor supply bottleneck of a fungus bioluminescence system is broken through, and an innovative solution is provided for creating efficient self-luminous plants.

Methods, compositions and uses for inhibiting the growth in blood cancer cells in a patient with one or more of a caffeic acid phenpropyl ester (GL8) analogue selected from the group consisting of As26, J229, J91, LL27, LL23, HM7, As25, MT26, and J205. The blood cancer cells can be myeloma, lymphoma and leukemia cells. The methods, compositions and uses can be in conjunction with the use of an IMiD to treat a patient. The compositions can include a pharmaceutically acceptable carrier, adjuvant or vehicle, a pharmaceutically acceptable salt or dietary supplement.

The invention provides a fingerprint spectrum and multi-component content determination method of a calamus-far nerve calming mixture, and relates to the field of quality control of traditional Chinese medicines. 31 common peaks are calibrated by establishing a fingerprint spectrum, and 24 common peaks can clearly belong to polygala tenuifolia. Meanwhile, synchronous content determination of seven components including chlorogenic acid, caffeic acid, ferulic acid, liquiritin, 2, 3, 5, 4 '-tetrahydroxy stilbene-2-O-beta-D-glucoside, 3, 6'-dimustard acyl sucrose and rosmarinic acid is realized. The method is good in precision, high in repeatability and stable and reliable in result, the medicine quality can be comprehensively and accurately reflected and controlled, and powerful support is provided for guaranteeing the safety and effectiveness of the calamus-far Ningmind mixture.

The invention discloses recombinant saccharomyces cerevisiae, a 11-seed oil fermentation product as well as a preparation method and application of the 11-seed oil fermentation product, and belongs to the technical field of synthetic biology and fermentation product preparation. According to the recombinant saccharomyces cerevisiae disclosed by the invention, a caffeic acid coenzyme A ligase 4CL coding gene, a chalcone isomerase CHI coding gene, a flavanone-3-hydroxylase F3H coding gene and a chalcone synthase CHS mutant coding gene are over-expressed, and the chalcone synthase CHS mutant is preferably Y69H / H71Y / Q161K. The preparation method of the 11-seed oil fermentation product comprises the following steps: fermenting a recombinant saccharomyces cerevisiae fermentation solution in the presence of a sophora flower bud extract and caffeic acid to obtain a first fermentation solution; fermenting the lactic acid bacteria fermentation liquor in the presence of 11 seed oil to obtain second fermentation liquor; and mixing the first fermentation liquid and the second fermentation liquid, continuing fermentation, and respectively collecting an oil phase and a water phase after fermentation is finished, so as to obtain a fermentation product. The product provided by the invention has multivitamin antioxidant activity.

The invention belongs to the technical field of organic synthesis and cosmetics, and particularly relates to a synthesis method of caffeoyl tripeptide-1. According to the invention, caffeic acid and tripeptide-1 are used as raw materials, and a condensation reaction is carried out under mild conditions through a specific activation strategy and a protective group technology to generate a target product. The method has the advantages of simple steps, mild reaction conditions, high total yield, good product purity and the like.

The present invention relates to a nanoparticle system comprising a metal selected from silver and gold, coated with polyethylene glycol (PEG) and functionalised with a polyphenol organic acid selected from caffeic acid, gallic acid and ferulic acid. The present invention also relates to a method for manufacturing said nanoparticle system and to the therapeutic and cosmetic use of said nanoparticle system.

The present application relates to the technical field of medicine, and particularly relates to a drug composition with a sequential synergistic repair effect and a preparation method and application thereof. The drug composition comprises liposome I encapsulating caffeic acid and liposome II encapsulating BH4; the liposome I is a ROS-responsive liposome, can specifically respond in a high ROS microenvironment, and then realize on-demand release of caffeic acid; meanwhile, the liposome II protects BH4 from entering cells to play a role, can effectively overcome the defects of difficult dissolution of caffeic acid and easy oxidation of caffeic acid and BH4, can improve the bioavailability of the two drugs, can realize sequential release of caffeic acid and BH4, and can play a synergistic treatment effect on different mechanisms of occurrence of radioactive skin damage, and provides a sequential synergistic repair strategy for clinic. The liposome I and the liposome II are both prepared by a thin film hydration method, and the operation is simple, the conditions are mild, the lipid film is uniformly formed, and the encapsulation rates of caffeic acid and BH4 are high.

The invention discloses polymer-based anti-crack concrete and a preparation method thereof, and relates to the technical field of concrete. When the polymer-based anti-crack concrete is prepared, caffeic acid and glycidyl methacrylate react to prepare a chain extender; the preparation method comprises the following steps: reacting isophorone diisocyanate with polyethylene glycol and a chain extender in sequence to prepare polyurethane, and then reacting with mercaptopropyltrimethoxysilane to prepare modified polyurethane; the preparation method comprises the following steps: reacting carboxylated cellulose nanofibers with 4-amino-2, 2, 6, 6-tetramethylpiperidine to prepare pre-modified nanocellulose, and reacting the pre-modified nanocellulose with isocyanate propyl triethoxy silane to prepare modified nanocellulose; uniformly mixing the fine aggregate, the coarse aggregate, cement, fly ash, the modified polyurethane, the modified nano cellulose, water, a water reducing agent and a defoaming agent, pouring into a mold, vibrating, standing, demolding and curing to obtain the polymer-based anti-crack concrete. The polymer-based anti-crack concrete prepared by the preparation method disclosed by the invention has good anti-crack and anti-aging properties.

The present application relates to a method for introducing an exogenous caffeic acid synthesis pathway to improve the light intensity of plants, and introducing TAL genes and P450 genes into plants to obtain a significant up-regulation effect of caffeic acid expression amount. This may be because the introduction of TAL genes and P450 genes promotes the deamination of L-DOPA, increases the accumulation amount of L-DOPA, and further effectively increases the caffeic acid expression amount in plants. Based on this pathway, the synthesis of luciferin in plants is increased to enhance the light intensity of plants.

The invention belongs to the technical field of traditional Chinese medicine detection, and particularly relates to a construction method of a specific chromatogram of caulis clematidis armandii or derivative products thereof. The invention provides a construction method of a characteristic chromatogram of caulis clematidis armandii or a derivative product thereof, which comprises the following steps: by taking octadecylsilane chemically bonded silica as a filler and a mobile phase comprising a formic acid-containing aqueous solution and acetonitrile, carrying out a specific gradient elution procedure to obtain 22 common characteristic peaks, and realizing effective separation of the common characteristic peaks, so that the separation degree is good, and the accuracy is high. The obtained characteristic spectrum has more characteristic peaks and better baseline separation, and is easier to position and qualitatively analyze; the precision, the stability and the repeatability are relatively good; compared with the prior art, the method has the advantages that the characteristic peaks are more, six characteristic components including the straight clematis seed B, the lariciresinol, the secoisolariciresinol, the lariciresinol-4-O-beta-D-glucoside, the caffeic acid and the ferulic acid are identified, and a basis is provided for quality detection and content determination of the caulis clematidis armandii or derivative products of the caulis clematidis armandii.

A method for preparing silkworm pupa active peptide microcapsules and their applications are disclosed. In a microfluidic focusing droplet generation chip, an aqueous phase is injected into the chip through the main channel, and an oil phase is injected through two side channels. The resulting microdroplets are collected in the collecting phase to form microcapsules. The filter membrane has a pore size of 0.1-1 μm. The aqueous phase consists of sodium alginate and silkworm pupa protein peptides; the oil phase consists of one of mineral oil, soybean oil, silkworm pupa oil, corn oil, peanut oil, rapeseed oil, and lard; the collecting phase consists of caffeic acid-grafted chitosan and calcium chloride dissolved in acetic acid. By optimizing the formulation and process, droplets with good dispersibility in oil, uniform morphology and size, and good stability are prepared.

This invention relates to the field of adhesive technology, and discloses an environmentally friendly biomass-based underwater adhesive, its preparation method, and its applications. The adhesive is prepared by one-step melt copolymerization of three biomass raw materials: lipoic acid, caffeic acid, and cysteine. This system utilizes the ring-opening polymerization of lipoic acid to form a dynamic disulfide bond network providing cohesive force, the catechol groups of caffeic acid to provide various interfacial interactions with the substrate, and the thiol groups of cysteine ​​to simultaneously participate in network construction and inhibit catechol oxidation, thereby synergistically achieving high-strength and durable underwater adhesion. The adhesive achieves an underwater adhesion strength of up to 5.5 MPa on iron sheets, remains stable in various acid, alkali, and salt environments, and can be repeatedly recycled through hot melting. This invention uses entirely bio-based raw materials, employs a simple and non-toxic process, and is suitable for underwater remediation, marine engineering, and other fields.

The invention belongs to the technical field of medicines, and particularly relates to application of an echinacea caffeic acid derivative to preparation of a medicine or a feed additive for treating animal periodontal diseases and a composition containing the echinacea caffeic acid derivative. The invention discloses application of an echinacea caffeic acid derivative in preparation of a medicine for treating animal periodontal diseases. Experiments prove that the echinacea caffeic acid derivative has the effects of improving feline gingivitis, gingival bleeding, gingival atrophy, alveolar bone loss and the like, can be used for treating and preventing animal periodontal diseases, and has smaller toxic and side effects while ensuring that a better periodontal disease treatment effect can be realized.

The invention discloses ROS (reactive oxygen species) responsive hydrogel loaded with hypoxia-induced exosome as well as a preparation method and application of the ROS responsive hydrogel. The ROS responsive hydrogel comprises an ROS responsive hydrogel matrix and a hypoxia-induced exosome dispersed in the ROS responsive hydrogel matrix, and the ROS responsive hydrogel matrix is prepared by uniformly mixing hyaluronic acid grafted with 3-aminophenylboronic acid and epsilon-polylysine grafted with caffeic acid. Under a high ROS environment, boric acid ester bonds in the hydrogel can be destroyed to release exosomes and caffeic acid, and the exosomes induced by hypoxia contain more factors for promoting tissue repair and regeneration and also have the competitive advantage of high uptake rate. The ROS responsive hydrogel loaded with the hypoxia-induced exosome can realize on-demand release of the exosome, delivers the exosome with endogenous competitive advantages to an ischemic infarction region and removes excessive active oxygen to play a role in efficiently repairing myocardial infarction.

The invention discloses a modified electrode, a preparation method of the modified electrode and application of the modified electrode in caffeic acid detection, and relates to the technical field of electrochemical detection.The preparation method of the electrode comprises the following steps that firstly, an Au nano solution is prepared; 2, preparing a polyaniline hollow nanotube suspension liquid; 3, pretreating the glassy carbon electrode; and 4, modifying the glassy carbon electrode by using gold nanoparticle-polyaniline. The modified electrode has the advantages of being simple in preparation process, high in detection stability and high in detection sensitivity, the application prospect is wide, and good research, development and application values are achieved.

The invention provides application of a phellinus polyketide synthase gene SvPKS1 in promoting bioluminescence of fungi or plants, the coding sequence of the SvPKS1 is shown as SEQ ID NO.1, and the fungi are fungi for producing caffeic acid. According to the application, the SvPKS1 and other genes (nnH3H, nnLuz and nnCPH) of an FBP fungus luminescence pathway are co-expressed in fungi or plants by utilizing a transgenic technology, so that the fungi or plants are promoted to emit light biologically. The invention discloses co-expression of the phellinus polyketide synthase gene SvPKS1 and fungus luminescence pathway genes nnH3H, nnLuz and nnCPH for the first time, promotes fungus or plant bioluminescence, and provides a key candidate enzyme gene for promoting fungus or plant sustainable luminescence.

The application discloses a kind of Shenkun Yangxue granules fingerprint and its construction method, belong to traditional Chinese medicine detection technical field.Different batches of Shenkun Yangxue granules are prepared into test sample solution;Respectively with Danshensu, ephedrine hydrochloride, syringin, protocatechuic aldehyde, coffee acid, calycosin-7-O-β-D-glucoside, loganin, comphoric acid, shisonin, delphinidin and luteolin and kaempferol control preparation single control solution;Test sample solution and control solution are analyzed by high performance liquid chromatography and high resolution mass spectrometry respectively, 18 characteristic peaks are screened out, and 12 kinds of chemical components are identified by standard comparison and fragment information analysis, to obtain Shenkun Yangxue granules fingerprint.The Shenkun Yangxue granules fingerprint established by the method can be used for the quality control detection of Shenkun Yangxue granules, which is conducive to the comprehensive monitoring of product quality, and ensures the consistency, controllability and stability of product quality.

The invention provides a herbicide for preventing and treating cyperus rotundus in cooperation with glyphosate. The herbicide is prepared from bacillus velezensis fermentation liquor, phenolic acid and glyphosate. The phenolic acid comprises any one or a combination of more of p-coumaric acid, cinnamic acid, p-hydroxybenzoic acid and caffeic acid. The bacillus velezensis fermentation liquor and phenolic acid components are introduced and form a synergistic effect with the glyphosate, so that the dosage of a single dosage of the glyphosate is reduced, the control effect is guaranteed, meanwhile, the potential pressure of chemical pesticide input on the ecological environment is reduced, and the environmental compatibility of a weeding system is improved.

The invention discloses a production method of a green coffee bean extract capable of improving alpha-glucosidase inhibitory activity. Cellulase and pectinase are used for improving the yield of chlorogenic acid and a derivative (CGAs) of chlorogenic acid, tannase and esterase are used for conducting structural modification on polyphenol and CGAs in green coffee bean extract clear liquid, and then lactobacillus rhamnosus, lactobacillus paracasei and bacillus subtillis form a composite strain for fermentation. According to the method, the proportion of CGAs components and the proportion of caffeic acid in the green coffee bean extract are improved by combining an enzymic method with the probiotics to ferment the green coffee bean extract, and the alpha-glucosidase inhibitory activity of CGAs and metabolites thereof is improved.

The invention discloses a synthesis method of limidoterol, and belongs to the technical field of medicine synthesis. The method comprises the following steps: coupling cyclobutanone oxime and a caffeic acid derivative under the catalysis of ferrous acetylacetonate, a chlorobenzene solvent and the protection of nitrogen to obtain a decarboxylated cyanogen alkylation intermediate; oxidizing the intermediate with m-chloroperoxybenzoic acid in dichloromethane in the presence of sodium carbonate to obtain a vicinal diol monoester intermediate; catalyzing by cobalt chloride in methanol, reducing by sodium borohydride, and protecting by Boc2O to obtain a cyclized precursor; cyclizing the precursor in tetrahydrofuran by using sodium hydride to obtain a cyclized intermediate; and performing deprotection to obtain the limiterol. According to the method, dangerous reagents and processes are not used, the safety is high, and no heavy metal residues exist; the cost is low, precious metals are not needed, the post-treatment operation is simple and convenient, a complex purification process is not needed, and the total yield reaches 21%; process conditions are mild, normal pressure and conventional heating modes are adopted, and industrial production is easy to realize.

The application discloses a construction method of a UPLC characteristic spectrum of a radix-lithospermi and application thereof, 1) preparation of a control medicinal material reference solution; 2) preparation of a control reference solution: a certain amount of protocatechuic acid, protocatechuic aldehyde, ferulic acid, rosmarinic acid, caffeic acid tetramer and danshensu are taken to prepare a mixed solution by adding methanol, serving as the control reference solution; 3) preparation of a test solution; 4) determination: the control medicinal material reference solution, the control reference solution and the test solution are respectively taken and injected into a liquid chromatograph to determine characteristic spectra, wherein the characteristic spectra include at least 12 characteristic peaks; 5) qualitative analysis of a sample to be determined is carried out by using the characteristic spectra obtained in the above steps. The characteristic spectrum includes 12 characteristic peaks, can effectively distinguish radix-lithospermi (Xinjiang radix-lithospermi) and its pseudo products of different origins, and embodies the integrity of quality control of radix-lithospermi.

The application provides a metal polyphenol nanoparticle, a preparation method thereof and application thereof in preparing a medicine for treating acute lung injury. The metal polyphenol nanoparticle is self-assembled from metal ions and polyphenols. The metal ions are selected from Mg 2+ , Zn 2+ , Mn 2+ or Cu 2+ . The polyphenols are selected from epigallocatechin gallate EGCG, caffeic acid CA, chlorogenic acid CGA, gallic acid GA and luteolin LUT. The metal polyphenol nanoparticle is used for preparing a medicine for treating acute lung injury induced by sepsis, pneumonia, severe trauma or inhalation injury. The medicine further comprises a pharmaceutically acceptable carrier. The metal polyphenol nanoparticle provided by the application has the advantages of simple preparation process, high biological safety, synergistically enhanced anti-inflammatory and antioxidant activities of polyphenols and metal ions, and is superior to polyphenols or metal ions alone.

This invention discloses a cultivation method for improving the drought resistance of sugar beet trees, belonging to the field of agricultural cultivation technology. Caffeic acid is sprayed onto both the front and back leaves of sugar beet trees in arid environments, and biochar is added to the cultivation soil. This invention achieves synergistic effects through the combined application of biochar and caffeic acid, using multi-target regulation. The improved water and nutrient supply provided by biochar provides a physiological basis for the antioxidant effect of caffeic acid, while the protection of photosynthetic structures by caffeic acid enhances the root system's utilization efficiency of the biochar-modified substrate, forming a positive feedback mechanism for multi-dimensional stress relief. The sugar beet cultivation method of this invention has proven to be highly effective in achieving high yield, high quality, and synergistic improvement in stress resistance.

The application discloses a caffeic acid-amino acid composite grafted starch and a preparation method and application thereof. The preparation method comprises the following steps: using a catalytic system comprising 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and 4-dimethylaminopyridine to catalyze caffeic acid to be selectively grafted to primary hydroxyl groups at C6 of high amylose starch, so as to prepare caffeic acid selectively grafted starch; then, amino acid is added to continue the reaction, so as to prepare caffeic acid-amino acid composite grafted starch; and the amino acid mainly reacts with secondary hydroxyl groups at C2 and C3 of the high amylose starch. The composite grafted starch in the application has excellent emulsification stability, gelation characteristics and biological activity by using the synergistic effect between functional groups, the prepared emulsion gel has an encapsulation rate of active substances of more than 93%, a storage stability of more than 90 days, and a significant enhancement of antioxidant capacity, and the inhibition rate of pathogenic bacteria is greater than or equal to 99.9%, so that the composite grafted starch can be widely applied to high-end skin care cosmetics such as face cream and sunscreen cream.

The invention provides self-healing hyaluronic acid-gelatin hydrogel as well as a preparation method and application thereof, and belongs to the technical field of hydrogel. According to the invention, modifiable sites of hyaluronic acid and gelatin are modified, and phenylboronic acid modified hyaluronic acid and caffeic acid modified gelatin are chemically crosslinked through boric acid ester to form the hydrogel, so that the problem of insufficient adhesive force of hyaluronic acid and gelatin is solved, and meanwhile, the hydrogel has self-healing ability to adapt to constantly changing wound tissues; the epigallocatechin gallate is introduced into a hydrogel system, so that the hydrogel has excellent biocompatibility and good oxidation resistance at the same time. In addition, the preparation method provided by the invention is mild in reaction condition and simple and convenient to operate, and can be widely applied to the fields of skin dressings, tissue processes and the like.

A method to synthesize a silver nanohybrid material. The method includes mixing a nitrate solution with a citrate solution to form silver nanoparticles (AgNPs). The method further includes esterifying a first mixture including octadecanoic acid, octadec-9-enoic acid, and octadeca-9,12-dienoic acid with caffeic acid in the presence of an acid catalyst and a solvent to form an unsaturated carboxylic acid mixture including first, second, and third acrylic acid derivatives. The method includes reacting the unsaturated carboxylic acid mixture with ethylene glycol to form a second mixture including first, second, and third ester derivatives. The method further includes mixing the AgNPs with the second mixture to form a third mixture. The method includes evaporating water from the third mixture to form the silver nanohybrid material. The silver nanohybrid material includes a AgNP core covered with the first, second, and third ester derivatives bonded to the AgNP core.

Nanoparticle system comprising a metal, coated with polyethylene glycol (PEG), which is functionalized with a polyphenol-type organic acid, manufacturing procedure and use of said system. The present invention relates to a nanoparticle system comprising a metal selected from silver and gold, coated with polyethylene glycol (PEG), which is functionalized with a polyphenol-type organic acid selected from caffeic acid, gallic acid, and ferulic acid. The present invention also relates to a process for manufacturing said nanoparticle system and to the therapeutic and cosmetic use of said nanoparticle system.

The present application relates to the technical field of pharmaceutical compositions, and provides a composition for improving immune function, a preparation and a preparation method and application thereof, comprising: 25-40% of chlorogenic acid, 5-12% of luteolin, 10-20% of isochlorogenic acid A, 3-8% of caffeic acid, 0.5-1% of a targeting ligand, 2-5% of a microecological component, and 5-8% of a nano-carrier, and application of the composition in preparation of medicines, health products and functional foods for improving immune function, and a preparation method of the above-mentioned medicine preparation, comprising the following steps: preparing a core particle; coating the core particle with a sustained-release enteric coating to obtain a microcapsule particle; filling the microcapsule particle into a capsule shell to obtain an enteric capsule; coating the enteric capsule with a stomach-dissolving coating liquid to obtain a capsule preparation; and achieving the effect of improving the whole gastrointestinal immune function by rapidly targeting anti-inflammation in the stomach and continuously targeting regulation in the intestinal tract, and greatly improving the action efficiency and immune regulation effect of the preparation.