322 results about "Caffeic acid" patented technology

The invention provides a metal polyphenol nanoparticle, a preparation method thereof and an application of the metal polyphenol nanoparticle in preparation of a medicine for treating acute lung injury, the metal polyphenol nanoparticle is formed by self-assembly of metal ions and polyphenol, the metal ions are selected from Mg < 2 + >, Zn < 2 + >, Mn < 2 + > or Cu < 2 + >, and the polyphenol is selected from Mg < 2 + >, Zn < 2 + >, Mn < 2 + > or Cu < 2 + >. The polyphenol is selected from epigallocatechin gallate (EGCG), caffeic acid (CA), chlorogenic acid (CGA), gallic acid (GA) and luteolin (LUT). The metal polyphenol nanoparticles are used for preparing a medicine for treating acute lung injury induced by sepsis, pneumonia, serious trauma or inhalation injury, and the medicine further comprises a pharmaceutically acceptable carrier. The metal polyphenol nanoparticles provided by the invention are simple in preparation process and high in biological safety, polyphenol and metal ions synergistically enhance the anti-inflammatory and antioxidant activity, and the metal polyphenol nanoparticles are superior to the single use of polyphenol or metal ions.

The invention discloses a gynecological antibacterial gel containing a carbomer component, and belongs to the technical field of gynecological nursing materials. The gel is prepared from 0.5 to 5 percent of plant extract microcapsules, 0.5 to 3 percent of carbomer, 2 to 10 percent of glycerol, 0.1 to 1.5 percent of triethanol and the balance of purified water. The plant extract microcapsule is prepared by compounding methacrylated silk fibroin and a caffeic acid hyaluronic acid graft, has temperature responsiveness, can improve the adhesive force on the surface of vaginal mucosa, and realizes slow release of the plant extract; the caffeic acid catechol hydroxyl is chelated with silver ions, a nano-silver monodisperse layer is formed on the surface of the microcapsule, and the antibacterial activity is improved. The composite plant extract can inhibit bacteria, repair mucous membranes and maintain the acidic microenvironment of the vagina. When the gel is prepared, the problem that the swelling efficiency of carbomer is low is solved through glycerol pre-grinding, and triethanolamine is added in three times to form a gradient cross-linked network. The problems that traditional gel is unstable in viscosity, poor in mucous membrane adhesiveness and prone to damaging vagina micro-ecology are solved, the antibacterial effect is good, and preservation is stable.

The invention relates to a mussel-like polylipoic acid-based hydrogel adhesive as well as a preparation method and application thereof, and the preparation method comprises the following steps: (1) uniformly mixing a sericin solution and a caffeic acid solution, then carrying out grafting reaction to obtain a reaction solution, and carrying out dialysis treatment and drying on the reaction solution to obtain sericin grafted caffeic acid; (2) adding lipoic acid into the lithium hydroxide solution, stirring until the lipoic acid is completely dissolved, then adding sericin grafted caffeic acid, and heating for reaction to obtain a PTALi-CAS mixture; and (3) cooling the PTALi-CAS mixture, so as to obtain the mussel-like polylipoic acid-based hydrogel adhesive. The dual-network PTALi-CAS hydrogel adhesive obtained in the invention realizes wet adhesion and rapid hemostasis, has excellent comprehensive performance, and is beneficial to providing a good repair environment for wounds as a wound dressing.

The invention relates to a special cable sheath material for trucks and a preparation method thereof, and belongs to the technical field of cable sheath materials, the special cable sheath material comprises the following raw materials: 40-55 parts of polyvinyl chloride, 15-20 parts of high vinyl polybutadiene rubber, 12-16 parts of a modified reinforcing material, 0.5-1.5 parts of a stabilizer, 1-3 parts of an anti-aging agent, 2-4 parts of a compatilizer and 4-8 parts of a filler; according to the technical scheme, the plant-based compound is combined with caffeic acid, epoxidation is performed, and the composite plasticizer is obtained; the composite plasticizer is combined with the silanized graphene oxide to obtain a reinforcing material; combining the reinforcing material with an amino compound to obtain a modified reinforcing material; mixing polyvinyl chloride, high vinyl polybutadiene rubber, the modified reinforcing material, a stabilizer, an anti-aging agent, a compatilizer and a filler, and performing extrusion granulation to obtain the special cable protective sleeve material for the truck. The mechanical property, the corrosion resistance and the aging resistance of the protective sleeve are integrally improved, and the environmental protection property is good.

The invention relates to the technical field of fermentation of edible and medicinal fungi, and discloses a phellinus igniarius fermentation method capable of producing high-yield Hispidin and an application of the phellinus igniarius fermentation method. The phellinus igniarius fermentation method comprises the following steps: inoculating a phellinus igniarius strain into a culture medium containing a copper ion compound, and fermenting. The phellinus igniarius mycelium obtained by adopting the fermentation method is rich in Hispidin, and can be widely used for preparing related medicines and health foods for protecting liver, resisting tumors, resisting viruses, resisting oxidation, reducing uric acid and the like. In addition, the invention also discloses a high performance liquid chromatography detection method for analyzing the content of Hispidin, by adopting the method, the content of Hispidin and precursor substances (such as protocatechuic acid, protocatechualdehyde, caffeic acid, osmundone and the like) in the phellinus igniarius leavening and derivative products thereof as well as phellinus igniarius mycelia can be effectively analyzed, and the accuracy of Hispidin detection is favorably improved.

The invention discloses a construction method and an identification method of characteristic chromatograms of a senecio scandens medicinal material as well as decoction pieces, standard decoction and formula granules thereof, and relates to the technical field of traditional Chinese medicine identification. The construction method of the specific chromatogram comprises the following steps: extracting a to-be-detected sample with methanol to obtain a test solution; the method comprises the following steps: dissolving 5-caffeoylquinic acid, chlorogenic acid, caffeic acid, 4-caffeoylquinic acid, rutin, hyperoside, isoquercitrin and 4, 5-dicaffeoylquinic acid with methanol to obtain a reference solution; and a liquid chromatograph is adopted for determination. A chromatographic column of the liquid chromatograph takes phenyl-methyl polysiloxane as a stationary phase, acetonitrile as a mobile phase A and 0.05 vol%-0.3 vol% formic acid aqueous solution as a mobile phase B for gradient elution. The information of the characteristic chromatogram constructed by the method is rich, and the quality of the senecio scandens medicinal material, decoction pieces, standard decoction and formula granules can be quickly and comprehensively reflected.

The invention relates to the technical field of traditional Chinese medicine research, in particular to a discovery method of a cough relieving and phlegm eliminating quality marker of cough relieving granules and the quality marker. According to the application, the cough-relieving and phlegm-eliminating drug effects of the cough-relieving granules are evaluated by virtue of concentrated ammonia water cough inducing and phenol red excretion tests, plasma pharmaceutical chemistry research of the cough-relieving granules is developed by virtue of a UHPLC-Q-Exactive Plus Orbitrap HRMS technology, and 26 prototype blood-entering components of the cough-relieving granules are analyzed and identified; through network pharmacological analysis of components entering blood, 11 potential active components for relieving cough and eliminating phlegm of the cough-relieving granules are screened, relate to 40 common targets such as IL6, TLR4, STAT3 and the like, and mainly participate in signal channels such as PI3K-Akt, HIF-1, EGFR (epidermal growth factor receptor) and the like; the seven prototype blood-entering components are quantitatively analyzed by utilizing pharmacokinetics. The three compounds of azelaic acid, caffeic acid and vanillin are used as the cough relieving and phlegm eliminating quality markers of the cough relieving granules through effectiveness, transitivity and testability. The research result is of great significance in deeply defining the pharmacodynamic material basis of the cough-relieving granules, optimizing the quality standard and promoting the clinical application of the cough-relieving granules.

The invention discloses application of a plant extract in preparation of a drug for resisting canine coronavirus, and relates to the technical field of biological medicine. Research finds that plant extracts-shikimic acid, phloretin, caffeic acid, tribulus terrestris saponin, berberine hydrochloride and apple polyphenol have activity of resisting canine coronavirus, wherein the effect of phloretin is most remarkable. The phloretin has antiviral activity in three aspects of direct killing, inhibition of virus adsorption and inhibition of virus growth, wherein the effect of inhibiting virus growth is most significant. And the EC50 of the phloretin acting on the CCoV is 0.616 mg / L. The plant extract provided by the invention is derived from natural plants, and has the advantages of low toxicity and good biocompatibility compared with chemical drugs. The invention provides a technical support for developing a novel, effective, safe and side-effect-free medicinal preparation for preventing and treating canine coronavirus.

The invention discloses a preparation method and application of a response type NO release anticoagulant hydrogel material. The preparation method comprises the following steps: providing methylacryloylated recombinant collagen; providing methacrylated hyaluronic acid; providing caffeic acid arginine amide modified hyaluronic acid; the response type NO release anticoagulant hydrogel material is obtained by taking methylacryloylated recombinant collagen, methylacryloylated hyaluronic acid and caffeic acid arginine amide modified hyaluronic acid as raw materials. The prepared hydrogel material has the advantages of resisting bacteria, promoting proliferation of endothelial cells, inhibiting proliferation of smooth muscle cells, resisting inflammation, promoting angiogenesis, inhibiting thrombosis and the like, arginine can serve as an endogenous NO donor in blood to release NO molecules, and therefore the effect of promoting vascular endothelial repair is achieved, and the hydrogel material has the advantages of being capable of resisting bacteria, promoting proliferation of endothelial cells, inhibiting proliferation of smooth muscle cells, resisting inflammation, promoting angiogenesis, inhibiting thrombosis and the like. The introduced NO molecules enable the hydrogel material to have good anticoagulant performance, and the hydrogel material is expected to be used for preparing an artificial blood vessel anticoagulant coating.

Disclosed are modified Type 1 O-methyltransferases (OMTs) and methods of using such modified OMTs. In some forms of the modified OMTs, the amino acid corresponding to Phe337 of Sorghum bicolor stilbene OMT (SbSOMT) is substituted with a polar amino acid. In some forms of the modified OMTs, the amino acid corresponding to Asn323 of Sorghum bicolor caffeic acid OMT (SbCOMT) is substituted with a hydrophobic amino acid. In some forms of the modified OMTs, the amino acid corresponding to Ile144 of SbSOMT is substituted with a polar amino acid. In some forms of the modified OMTs, the amino acid corresponding to Asn128 of SbCOMT is substituted with a hydrophobic amino acid.

The present invention relates to the field of plant extraction, and in particular to an extraction process for skin care active ingredients in coffee peels. The present invention adopts a natural and pollution-free coffee peel extraction method, and extracts the natural active chemical components in the coffee peels through enzymatic biomimetic extraction, which can achieve the simultaneous extraction of chlorogenic acid, caffeic acid, and resveratrol in the coffee peels. The obtained extraction product has a high content of skin care active ingredients, which can be used as a cosmetic raw material to exert anti-inflammatory, anti-oxidation, whitening, soothing and other skin care effects; this technology makes full use of the production waste of coffee peels, reduces environmental pollution, recycles resources, and improves economic benefits.

The invention belongs to the technical field of polymer synthetic materials, and particularly relates to bio-based MS resin and a preparation method thereof. The bio-based MS resin is a free radical copolymer of a methyl methacrylate monomer and a styrene monomer; the methyl methacrylate monomers comprise bio-based methyl methacrylate, isobornyl methacrylate and 3-pinane methacrylate, and the methyl methacrylate monomers comprise methyl methacrylate, isobornyl methacrylate and 3-pinane methacrylate; the styrene monomers comprise styrene and caffeic acid derivatives; based on 100% of the mass of the bio-based MS resin, the methacrylate monomer accounts for 30%; the molar ratio of caffeic acid derivatives to styrene in the styrene monomers is 1: (1-9). The synthesized bio-based MS resin has the characteristics of greenness and reproducibility, compared with petroleum-based MS resin, the performance of the MS resin can be regulated and controlled by designing a protecting group of a bio-based caffeic acid derivative so as to expand the application range of the MS resin, a specific functional group can be introduced to prepare a functional, advanced and high-performance polymer material, and the MS resin has a good market prospect.

The invention discloses a hydrogel plugging umbrella for gastric perforation and a preparation method thereof. The hydrogel plugging umbrella comprises a plugging part, and fixing parts are integrally formed at the two ends of the plugging part. The excellent plugging effect is achieved through the triple synergistic effect, the space mechanical interlocking effect brought by the double-umbrella-shaped design provides the immediate plugging and continuous self-expansion compaction effect, and physical and chemical adhesion of a tissue-gel interface ensures that under the corrosive gastric juice environment and the dynamic mechanical load, the plugging effect is good, and the plugging effect is good. A safe and lasting gastric perforation sealing effect can still be provided; the caffeic acid modified chitosan component has unique proton adsorption and biomolecule capture characteristics and can effectively block permeation of digestive enzymes such as gastric acid and pepsase, the protection effect not only maintains the activity of endogenous growth factors in a wound microenvironment, but also creates conditions beneficial to tissue regeneration, and the wound healing effect is improved. Therefore, healing and repairing of gastric perforation are remarkably promoted.

The invention discloses a nano-drug material based on self-assembly of berberine and caffeic acid and a preparation method of the nano-drug material. The preparation method comprises the following steps: respectively dissolving berberine and caffeic acid in an organic solvent to prepare a berberine-organic solvent solution and a caffeic acid-organic solvent solution; adjusting the pH value of the caffeic acid-organic solvent solution to 7.0-7.5; adding the caffeic acid-organic solvent solution while stirring the berberine-organic solvent solution, uniformly mixing, adding the mixed solution into deionized water with the preheating temperature of 30-70 DEG C, and stirring at a constant temperature, so that the berberine and the caffeic acid are self-assembled to obtain a mixed solution; dialyzing the mixed solution to obtain a berberine and caffeic acid self-assembled solution; and performing vacuum freeze drying to obtain a target product. The nano-drug material disclosed by the invention is small in particle size, better in solubility and dissolution rate in different pH environments than berberine hydrochloride and caffeic acid eutectic, good in stability in an aqueous solution and environment-friendly in method.

The invention belongs to the field of quality analysis and control of traditional Chinese medicines, and discloses a method for determining the content of carpesium abrotanoides components, which comprises the following steps: by taking isochlorogenic acid A as an internal reference substance, calculating the content of chlorogenic acid, caffeic acid, galuteolin, 11 (13)-dehydroaxillary Ivalin, isochlorogenic acid C, 2, 3, 4, 5-tetramethyl-1, 3, 4, 5-tetramethyl-1, 3, 5-tetramethyl-1, 3, 5-tetramethyl-1, 3, 5-tetramethyl-1, 3, 5- According to the method, the content of 10 components of carabrone is rapidly and accurately detected according to the relative correction factors of 2, 3, 4, 5-tetracaffeoyl-D-glucaric acid, 2-deoxy-4-surface-gaillanin, carabrone and dellelactone, and the problem that the detection cost is high due to the fact that many reference substances are needed, are not easy to obtain and are high in price in carabrone content determination is solved.

The invention relates to a preparation method of a mixture of sorghum starch and caffeic acid. The preparation method is characterized by comprising the following steps: mixing water, sorghum starch and caffeic acid to prepare a sorghum starch-caffeic acid mixed solution; then carrying out microblog treatment on the sorghum starch-caffeic acid mixed solution; after the treatment is finished, cleaning with an ethanol solution to remove free caffeic acid components in a reaction system, and centrifuging to take white precipitate at the lower layer; and freeze-drying to obtain a mixture of sorghum starch and caffeic acid. The SS-CA compound provided by the invention has a more stable gel continuous phase in the aging process. At the same aging time point, the moisture distribution of the SS-CA composite system has a lower free water peak area proportion, and the moisture retention capability is improved.

The invention discloses a method for improving the self-luminous intensity of a plant, which is characterized in that an AroGL175Q gene or an RgTAL gene and a PtC3H gene or the AroGL175Q gene, the RgTAL gene and the PtC3H gene are expressed in a plant containing a fungus bioluminescence system FBP, so that a transgenic plant of which the caffeic acid content, the milk tree alkali content and the luminous intensity are obviously increased is obtained. According to the invention, through metabolic network optimization and transformation, phenylpropane metabolites required by middle and upper streams of a fungus bioluminescence pathway are increased, and synthesis of caffeic acid and milk tree alkali in plants is increased, so that bioluminescence enhancement is realized. The precursor supply bottleneck of a fungus bioluminescence system is broken through, and an innovative solution is provided for creating efficient self-luminous plants.

The invention relates to a dual-targeting fluorescent carbon dot for endoplasmic reticulum and lipid droplets and a preparation method and application thereof.The preparation method comprises the steps that o-phenylenediamine and phenylalanine are dissolved in pure water to obtain a first dissolved solution; transferring the first dissolving solution into a poly-tetrafluoroethylene lined reaction kettle, carrying out a first heating reaction to obtain a precipitate, and sequentially carrying out column chromatography purification, rotary evaporation and drying on the precipitate to obtain a target solid; dissolving caffeic acid in dichloromethane, adding CDI, and stirring to obtain a second dissolved solution; and adding the target solid into the second dissolving solution, and carrying out a second heating reaction to obtain the dual-targeting fluorescent carbon dots for the endoplasmic reticulum and the lipid droplets. The dual-targeting fluorescent carbon dots obtained by the preparation method can distinguish endoplasmic reticulum and lipid droplets, and solve the problem of lack of life-sensitive dual-targeting fluorescent carbon dots capable of simultaneously positioning the endoplasmic reticulum and lipid droplets and sensitively monitoring microenvironment changes of the endoplasmic reticulum and lipid droplets.

The invention discloses a caffeic acid-O-methyltransferase 2 function acquired mutant, a recombinant expression vector and application thereof, and belongs to the technical field of crop biological breeding. It is found for the first time that after the 344 amino acid of tomato caffeic acid-O-methyltransferase 2 is changed into tryptophan (W) from cysteine (C), the melatonin content of tomatoes can be increased, the tomatoes can be endowed with broad-spectrum resistance to abiotic and biological stress, and the tomato caffeic acid-O-methyltransferase 2 can serve as a key target for tomato resistance breeding.

The invention discloses a redness repairing and relieving composition as well as a preparation method and application thereof. The red repairing and relieving composition comprises a red repairing and relieving functional agent, wherein the components of the red repairing and relieving functional agent comprise a phenolic compound and a phenolic acid compound; the phenolic compound is selected from at least one of hydroxytyrosol and tyrosol; the phenolic acid compound is selected from at least one of caffeic acid, protocatechuic acid, plateau catechuic acid and ethyl protocatechuate. The composition can inhibit release of inflammatory factors, relieve itching, resist inflammation, calm skin and reduce angiogenesis, and meanwhile can enhance the skin water locking capacity, improve the skin water shortage condition and enable the skin to recover to be moisturized, tender and smooth.

The invention discloses a heterozygote of 3-hydroxylase and application of the heterozygote in production of levodopa, caffeic acid or hydroxytyrosol, and relates to the technical field of biology. The heterozygote of the 3-hydroxylase comprises HpaB and HpaC, and the 3-hydroxylase comprises HpaB and HpaC. The limitation of the catalytic efficiency of hydroxylase in the prior art is overcome, the production efficiency of levodopa, caffeic acid or hydroxytyrosol is remarkably improved, and the method has a very good application prospect. The expression levels of HpaB and HpaC are properly regulated and controlled by using connecting element sequences such as a connecting peptide sequence and a 5 '-UTR sequence, so that a better production effect can be obtained.

The invention provides a preparation method of a metal organic coordination polymer mediated silver nanowire. The preparation method comprises the following steps: respectively preparing a supramolecular assembly agent, a high-molecular polymer protective agent, halide salt and a soluble silver salt solution; the supramolecular assembly agent is at least one of cinnamic acid, p-coumaric acid, caffeic acid, tannin and the like; halide salt and a supramolecular assembly agent are sequentially added into the soluble silver salt solution to form a silver ion-supramolecular assembly agent coordination polymer solution; adding a high-molecular polymer protective agent into the silver ion-supramolecular assembly agent coordination polymer solution, stirring, and reacting in a dark place at-20 to 80 DEG C for 0.1 to 10 hours; and after the reaction is completed, centrifuging, washing, drying in vacuum and re-dispersing to obtain the silver nanowire. According to the technical scheme, the prepared silver nanowire is superfine in diameter, the end has a curled shape, the interior contains defects, the reaction can be carried out in water, the productivity is high and is larger than 80 g / L, and the technology is simple and more environmentally friendly.

The invention provides a water-based environmentally friendly anti-corrosion coating, comprising the following raw materials in parts by weight: 50-60 parts of water-based composite emulsion, 4-10 parts of ketimine; 4-6 parts of polyaniline intercalated graphene, 16-18 parts of composite nanoparticles loaded with corrosion inhibitors and 5-10 parts of auxiliary agents; the water-based composite emulsion consists of sodium alginate / acrylic acid / acrylamide copolymer emulsion and water-based epoxy resin emulsion; the auxiliary agent comprises hydroxyethyl cellulose and expanded vermiculite powder; the composite nanoparticles are porous silica / titanium dioxide composite nanoparticles; the corrosion inhibitor consists of histidine, anthocyanin and caffeic acid; the invention uses the water-based composite emulsion as a matrix, adds polyaniline intercalated graphene, composite nanoparticles loaded with corrosion inhibitors and auxiliary agents thereto; and uses ketimine as a curing agent to prepare an environmentally friendly water-based environmentally friendly anti-corrosion coating with long-term anti-corrosion performance.

A method for preparing a sustained-release antibacterial film includes: (1) mixing a cyclodextrin metal-organic framework (CD-MOF) material with a silver nitrate short-chain alcohol solution to prepare an intermediate material A; (2) mixing the intermediate material A with a caffeic acid short-chain alcohol solution to prepare an intermediate material B; (3) dispersing the intermediate material B in a solvent, adding a polydimethylsiloxane main agent and a PDMS auxiliary agent in sequence, and mixing well by stirring to obtain a casting solution; and (4) coating the casting solution on a film support material, conducting vacuum drying, and peeling off a film from the film support material to obtain the sustained-release antibacterial film. A mixed matrix-based film material in which the CD-MOF and the PDMS are physically blended is prepared based on CD-MOF and PDMS for the first time, and may be used in application researches of food, environment and other fields.

The invention belongs to the technical field of extraction of medicinal chemical components from plants, and discloses a thesium chinense extracting solution as well as a preparation method and application thereof, and the preparation method comprises the following steps: drying thesium chinense at low temperature, crushing and sieving to obtain thesium chinense powder; mixing the thesium chinense powder with an extracting agent, and extracting to obtain a crude extracting solution; and centrifuging and filtering the crude extracting solution to obtain the thesium chinense extracting solution. The extracting agent is a deep eutectic solvent or a deep eutectic solvent water solution. The deep eutectic solvent composed of betaine, caffeic acid and butanediol is used, the flavonoid glycoside compounds in thesium chinense can be selectively recognized, the flavonoid glycoside compounds in thesium chinense are effectively extracted, and the yield is increased. The betaine, the caffeic acid, the butanediol and the like are biocompatible reagents, so that the thesium chinense extracting solution can be directly prepared, the processes of concentration and redissolution are avoided, the sample preparation process and the use of organic solvents are simplified, and the thesium chinense extracting solution is more environment-friendly and has higher biological activity.

Methods, compositions and uses for inhibiting the growth in blood cancer cells with one or more of caffeic acid (3,4-dihydroxycinnamic acid) phenethyl ester (CAPE), CAPE analogue MT30, and CAPE analogue GL8. The blood cancer cells can be myeloma, lymphoma and leukemia cells. The methods, compositions and uses can be in conjunction with the use of an IMiD to treat a patient. The compositions can include a pharmaceutically acceptable carrier, adjuvant or vehicle, a pharmaceutically acceptable salt or dietary supplement.

The present invention relates to a recombinant microorganism comprising a heterologous nucleic acid sequence coding for a 4-coumaroyl-CoA ligase (4CL), a heterologous nucleic acid sequence coding for a coumaroyl-CoA 3-hydroxylase (CCoA3H) and a heterologous nucleic acid sequence coding for an acyl-coenzyme A thioesterase. The invention additionally relates to a recombinant microorganism comprising a heterologous nucleic acid sequence coding for a caffeic acid O-methyltransferase. It also relates to the use of these microorganisms for producing ferulic acid and / or caffeic acid and to methods for producing caffeic acid and / or ferulic acid using said microorganisms.

Methods, compositions and uses for inhibiting the growth in blood cancer cells in a patient with one or more of a caffeic acid phenpropyl ester (GL8) analogue selected from the group consisting of As26, J229, J91, LL27, LL23, HM7, As25, MT26, and J205. The blood cancer cells can be myeloma, lymphoma and leukemia cells. The methods, compositions and uses can be in conjunction with the use of an IMiD to treat a patient. The compositions can include a pharmaceutically acceptable carrier, adjuvant or vehicle, a pharmaceutically acceptable salt or dietary supplement.

The invention discloses an application of caffeic acid-O-methyltransferase in regulation and control of salt tolerance of rice. The overexpressed OsCOMT gene disclosed by the invention can remarkably improve the melatonin content and antioxidant level of rice seedlings, so that the tolerance of plants to salt stress is enhanced, and the plants can maintain normal growth under the condition of salt stress; the deletion of the gene OsCOMT leads to the increase of the sensitivity of seedling-stage rice to salt stress, and exogenous melatonin can recover the salt-sensitive phenotype of a mutant-knocked-out plant and improve the photosynthetic rate of the mutant-knocked-out plant. The OsCOMT gene and the encoding protein thereof have important theoretical and practical significance on research of a plant stress tolerance mechanism and improvement of stress tolerance and related traits of plants, can play an important role in plant stress tolerance gene engineering improvement, and are wide in application prospect.

The invention provides a method for preparing a polyvinyl alcohol flame-retardant film by using coffee shell carbon dots and application, and belongs to the technical field of composites.The method comprises the following steps that a coffee shell carbon dot solution and polyvinyl alcohol are mixed, heated in an oil bath and then cooled to the room temperature, the cooled coffee shell carbon dot-polyvinyl alcohol solution is subjected to tape casting to form a film, and the polyvinyl alcohol flame-retardant film is obtained. And drying to obtain the coffee shell carbon dot-based polyvinyl alcohol flame-retardant film. The coffee shells are used as the raw material to prepare the carbon dots, caffeic acid and chlorogenic acid in the coffee shells are mainly used, resource utilization of waste is achieved, the production cost of the flame-retardant film is reduced, and the prepared flame-retardant film is light, has certain mechanical strength and is suitable for various fields needing light flame-retardant materials. The flame retardant property is excellent, flame spreading can be effectively delayed or prevented, the safety of the material is improved, and the preparation method is simple and is more suitable for popularization and application.