205 results about "Melatonin" patented technology

The invention relates to the technical field of exosomes, in particular to an exosome compound preparation with a hair growing effect and a preparation method of the exosome compound preparation. Comprising 12 to 22 parts of ginsenoside Rb1, 15 to 12 parts of biotin tripeptide-15, 8 to 15 parts of diammonium glycyrrhizinate, 50 to 70 parts of fish collagen peptide, 10 to 20 parts of soybean peptide, 0.5 to 4.5 parts of SHH protein, 0.5 to 2.5 parts of SMO inhibitor, 0.5 to 1.5 parts of HhSP protein, 5 to 10 parts of N-acetyl tyrosine, 0.1 to 2 parts of hyaluronic acid, 0.1 to 1 part of melatonin, 0.1 to 1 part of quercetin, 0.1 to 1 part of kaempferol, 0.1 to 1 part of tanshinone, 0.1 to 1 part of biotin and 0.1 to 1 part of vitamin A. The compound preparation is combined with exosomes from various sources, so that the hair papilla cell function is synergistically enhanced, and the damaged hair follicle structure is repaired.

The invention discloses a compound composition for improving sleep disorders, which is characterized by comprising the following components: an inhibitory neurotransmitter component, melatonin and a nano-lipid carrier, the nano-lipid carrier comprises phospholipid and cholesterol, the weight ratio of phospholipid to cholesterol is (1-6): 1, the weight ratio of phospholipid to cholesterol is (1-6): 1, and the weight ratio of melatonin to cholesterol is (1-6): 1. The inhibitory neurotransmitter component is wrapped in the nano-lipid carrier to form lipid nanoparticles, and the weight ratio of the inhibitory neurotransmitter component to the nano-lipid carrier is 1: (1-15). The compound composition can be prepared into a compound oral mucosa preparation, can be rapidly disintegrated when encountering saliva in the oral cavity, is good in patient compliance and small in side effect, all the components play a synergistic effect from different mechanisms through multiple target points, and the compound oral mucosa preparation can improve the sleep quality, prolong the sleep time and shorten the sleep latency; and patients with sleep disorders are helped to improve insomnia from the pathologic root.

The invention discloses a melatonin rapidly disintegrating oral tablet and a preparation method thereof, and the melatonin rapidly disintegrating oral tablet is prepared from the following raw materials in parts by weight: 40 to 55 parts of mannitol, 3 to 6 parts of cross-linked povidone, 0.5 to 2.0 parts of povidone, 2 to 6 parts of melatonin, 30 to 45 parts of filler and 0.3 to 1.5 parts of lubricant. Wherein the mannitol is beta-crystal form mannitol. According to the invention, mannitol of a limited type is matched with raw materials such as polyvinylpolypyrrolidone, and meanwhile, a specific mixing and tabletting process is adopted, so that the tablet can be rapidly disintegrated in an oral cavity while the hardness of the tablet is maintained, the bioavailability is high, and the requirements of the health food field on taking convenience, effectiveness and industrial production can be met.

The invention relates to the technical field of plant stress regulation and medicinal plant cultivation. A salt stress damage repair method for cutting seedlings of aromatic medicinal plants comprises the following steps: after asexual cutting seedlings of the aromatic medicinal plants suffer from salt stress, spraying a melatonin aqueous solution with the concentration of 50-200 [mu] mol.L to the leaves of the seedlings in a repair window period of 3-6 days after the salt stress occurs, spraying 1-2 times a day, continuously spraying for 2-3 days, and continuously spraying the melatonin aqueous solution with the concentration of 50-200 [mu] mol.L to the leaves of the seedlings in the repair window period of 3-6 days after the salt stress occurs. Melatonin is used for repairing salt damage of the asexual cuttage seedlings of the aromatic medicinal plants. The melatonin can be accurately applied before the salt damage critical point to repair the physiological damage of the cutting seedlings of the aromatic medicinal plants, and the salt damage influence of the cutting seedlings of the aromatic medicinal plants is greatly reduced; the problems that cutting seedlings of the aromatic medicinal plants are prone to salt damage under salt stress, the survival rate is low, and the content of main medicinal components is reduced are solved, and the dual effects of seedling physiological restoration and medicinal component content stabilization can be achieved.

The invention discloses a method for promoting biomass accumulation of haematococcus pluvialis, which comprises the following steps: adding salicylic acid, melatonin and alpha-ketoglutaric acid into a basic culture medium, and replacing ferric trichloride with ferric citrate as an iron source to obtain an improved BG-11 culture medium; the method comprises the following steps: inoculating a haematococcus pluvialis seed solution into a culture bottle containing an improved culture medium, carrying out illumination treatment on the first day to the second day by adopting pulsed red light with the wavelength of 620 nm in the early stage of a green stage, carrying out illumination treatment on the third day to the fifth day by adopting red light with the wavelength of 660 nm in the middle stage of the green stage, and carrying out illumination treatment on the sixth day to the seventh day by adopting pulsed red light with the wavelength of 680 nm in the later stage of the green stage. Through a synergistic scheme of the improved BG-11 culture medium, staged red light treatment and dynamic environment regulation and control, the problems of low light energy utilization rate and insufficient stress resistance in the existing haematococcus pluvialis culture process are solved, high efficiency and stability of haematococcus pluvialis biomass accumulation are realized, the biomass can reach 7.0 * 10 < 5 > cells / mL in the later period of the green stage, and the yield of the haematococcus pluvialis can reach 7.0 * 10 < 5 > cells / mL in the later period of the green stage. And the survival rate of haematococcus pluvialis cells reaches 95%.

The invention relates to the technical field of plant conditioners, in particular to application of nano silicon dioxide in relieving stress of polystyrene microplastics on hibiscus mutabilis. The invention finds that the influence of polystyrene micro-plastic on the stress of hibiscus mutabilis can be relieved to a certain extent during treatment by nano-silica, the intervention of the nano-silica causes the increase of secretion of melatonin, and the combined action of the nano-silica and melatonin makes the effect more remarkable and shows a synergistic effect. The nano silicon dioxide directly acts on the surfaces of cells to form a protective layer, so that adsorption and damage of PS-MPs to the cells are reduced, synthesis of melatonin is promoted, and a melatonin signal channel is activated. The activated melatonin signaling pathway further regulates the activity of carbon and nitrogen metabolism key enzymes, promotes carbon and nitrogen metabolism, improves synthesis and accumulation of carbohydrates and proteins, enhances the activity of an antioxidant system, removes active oxygen and reduces oxidative damage.

The invention discloses a multi-mode light-supplementing sleep-aiding control circuit, device and system integrating sound, light and smell, and a control method, and relates to the technical field of electronic circuits. The circuit comprises a main control module, a light supplementing module, a sleep-aiding lamplight module, an environment sensing module, an aromatherapy module, a power amplifier loudspeaker module and the like, low-blue-light soft and brightness-adjustable illumination is output through the light supplementing module, and the problems of too strong blue light, screen stroboflash and reading fatigue when an intelligent terminal is used are solved. The sleep-aiding light module can be adjusted to warm color temperature, the mood is relaxed, and illumination secreted by melatonin is not damaged. The environment sensing module collects environment light and proximity distance information in real time, so that the equipment can intelligently adjust the light supplementing brightness and the sleep-aiding lamp mode; hot fumigation closed-loop control is adopted for the aromatherapy module, constant-temperature adjustment is achieved through temperature sampling, and volatilization of sleep-aiding fragrance is more stable and safer; the power amplifier loudspeaker module provides stable output for white noise, natural sound and rhythmic respiration guiding sound, and provides reliable acoustic assistance for sleep adjustment.

The application belongs to the field of biology and discloses a rice OsCSA gene and a method for improving the copper stress resistance of rice, and more specifically discloses OsCSA application of the gene in improving the copper stress resistance of rice, through transcriptome analysis of rice under copper stress and verification under different experimental conditions under copper stress, it is found that OsCSA the gene has a close relationship with accumulation of melatonin in rice, and it is proved that OsCSA the gene and the melatonin have the ability to improve the copper stress resistance level of rice. Meanwhile, the application also discloses OsCOMT9 application of the gene and / or OsCOMT12 application of the gene and a method for improving the copper stress resistance of rice.

The present application belongs to the technical field of plant tissue culture, and particularly relates to a high-efficiency rooting and seedling raising method for plant tissue culture seedlings, S1 trimming and disinfection of S2 induction liquid immersion, S3 composite substrate implantation and gradient environment regulation, S4 substrate transplanting and planting; the core is to use modified magnetic biochar composite substrate loaded with FeO nanoparticles, cooperate with the synergistic effect of microencapsulated rooting hormone and melatonin, realize in-situ integrated seedling raising through composite spectrum containing UV-A, CO pulse supply, gradient humidity and PEG osmotic pressure domestication. The present application effectively inhibits basal browning, shortens the seedling raising period, improves the rooting quality and transplanting survival rate, overcomes the pollution prejudice of magnetic nanoparticles, is suitable for woody and herbaceous plants, and can be applied to plant tissue culture seedling production on a large scale.

The invention provides application of platanus sp. Su100 in promotion of synthesis of poplar melatonin and disease resistance, and relates to the technical field of microbial resource development and forest cultivation. The soft disc fungus Su100 can activate the activity of a key enzyme for synthesizing melatonin in a poplar body, and the content of the melatonin is remarkably increased. As a plant endogenous antioxidant and a multifunctional signal molecule, the melatonin plays a core role in plant stress resistance, growth regulation and control, quality improvement and ecological restoration. The floppy disc fungi have high colonization efficiency in plants, and can form a symbiotic system with the poplar by utilizing a floppy disc fungi inoculation technology to play a role for a long time. The application of the invention can significantly reduce the production cost of agriculture and forestry, is especially suitable for crop cultivation under the adverse conditions of drought, salt and alkali, diseases and the like, reduces the application of chemical fertilizers and pesticides, and lays a foundation for the construction of an efficient, green and sustainable forestry planting and cultivation system.

Provided herein are compositions for the prevention and treatment of demodex blepharitis in a subject and processes of treatment or prevention of demodex blepharitis. The compositions may include at least one avermectin compound selected from 5-O-demethyl-22,23-dihydroavermectin A1a or 5-O-demethyl-25-de(1-methylpropyl)-22,23-dihydro-25-(1-methylethyl)avermectin A1a, or a combination thereof, and melatonin, wherein an aggregate concentration of the avermectin compound is optionally less than 1 wt %. Further devices are provided that include wipe including a woven or non-woven membrane and a treatment composition, the treatment composition including melatonin, one or more anti-microbial compounds, and optionally one or more pharmaceutically acceptable excipients and processes of treatment or prevention of demodex blepharitis in a subject using the wipe, alone or optionally further with the composition as provided herein.

The invention discloses application of spermidine in in-vitro culture of human unfrozen oocytes and embryos. By adding the combination of spermidine, melatonin and astaxanthin into the thawed oocyte resuscitation culture solution and the embryo in-vitro culture solution, the mitochondrial autophagy and mitochondrial functions of the thawed oocytes are improved, so that the in-vitro culture effect of the thawed oocytes and embryos is improved, and the success rate of an assisted reproduction technology is increased.

The invention discloses a multi-system function regeneration factor composition as well as a preparation method and application thereof. The multi-system function regeneration factor composition is prepared from the following components in parts by mass: 0.8 to 2.4 parts of melatonin, 84 to 126 parts of nicotinamide ribose and 42 to 84 parts of resveratrol. According to the multi-system functional regeneration factor composition disclosed by the invention, through scientific proportioning and synergistic effect of melatonin, nicotinamide ribose and resveratrol, the functions of stem cells are strongly activated, proliferation of the stem cells is remarkably promoted, the stemness maintaining capability of the stem cells is improved, osteogenesis and adipogenesis differentiation potential is enhanced, and the regeneration effect of the stem cells is improved. Meanwhile, an NAD < + > metabolic pathway is activated so as to strengthen cell energy and anti-aging capability. The capsule adopts the design of combining quick-release pellets and sustained-release pellets, the quick-release part can quickly release melatonin, the local drug concentration can be increased in a short time, and stem cell activation signals can be quickly started; and the sustained release part slowly releases nicotinamide ribose and resveratrol to maintain the long-acting stability of the drug concentration, so that the effect taking speed is ensured, the action time is prolonged, and the bioavailability is greatly improved.

This invention belongs to the field of biotechnology and discloses a dry film stabilizer, cytoplasmic antigen dry films, their preparation methods, and applications. The dry film stabilizer includes polyethylene glycol, trehalose, BSA, melatonin, and Tween-20. Cytoplasmic antigen dry films are prepared using this stabilizer and applied in CBA detection. This dry film stabilizer has good permeability, penetrating the cell membrane to enter the cell interior, providing protection both inside and outside the cell. It also possesses moisturizing and antioxidant properties, preventing cell deformation and shedding of the cell fixation layer, effectively maintaining the stability and reliability of intracellular antigen proteins. The prepared cytoplasmic antigen dry films can be stored long-term without damaging the antigenicity of cytoplasmic signal indicators. When detecting positive samples, strong and numerous green filamentous fluorescence signals are observed in the cytoplasm, with consistent green and red fluorescence intensities. Software synthesis reveals co-localization of green and red antigen fluorescence.

The invention relates to the field of genetic engineering and fermentation engineering, and discloses a genetically engineered bacterium for producing melatonin and application of the genetically engineered bacterium in fermentation production of melatonin. According to the invention, genes of mtrA, PTPS, SPR, PCD and DHPR of related enzymes synthesized by coding 2-amino-6-(1, 2-dihydroxypropyl)-5, 6, 7, 8-tetrahydro-4 (1H)-pterin diketone and genes of L-2-amino-3 (beta-indole) propionic acid monooxygenase, oxymethyltransferase, N-acetyltransferase and decarboxylase are co-expressed on the genetically engineered bacterium, so that the gene engineering bacterium is obtained. The strain can be used for fermentation production of melatonin, and experiments show that the yield of the strain can reach 6480 mg / L.

The application discloses a CKD and IgAN early detection method based on blood mass spectrum analysis, utilizes liquid chromatography-tandem mass spectrometry technology to detect 24 kinds of compounds of a plasma sample in a targeted manner, utilizes a logic regression to establish an early detection model of CKD and IgAN, and verifies the prediction accuracy of CKD and IgAN in a cohort. Through analysis of plasma samples of healthy controls and CKD patients (including IgAN and non-IgAN patients), it is proved that the melatonin or melatonin+tryptophan level in the plasma is an effective method for detecting early CKD, and the melatonin, tryptophan, indole-3-lactic acid, indole-3-carboxylic acid, kynurenic acid and indole-3-methyl aldehyde are effective methods for detecting early IgAN. The application provides effective blood markers for early detection of CKD and IgAN.

It is an object of the present invention to provide a composition in a solid form, preferably in the form of a multilayer gummy with differentiated release of active ingredients.

This invention discloses a sleep-aid composition that synergistically regulates sleep rhythm with 5-HTP and L-theanine, relating to the fields of biomedicine and health food technology. This invention effectively regulates sleep rhythm through the synergistic effect of 5-hydroxytryptophan, L-theanine, and γ-aminobutyric acid (GABA), helping users improve sleep quality. 5-HTP, as a precursor to serotonin, helps increase serotonin levels, which are then converted into melatonin, regulating the sleep cycle. L-theanine promotes the release of alpha brain waves, helping to relax the mind and body and reduce anxiety. GABA, as an inhibitory neurotransmitter, effectively relieves tension and promotes deep sleep. The synergistic effect of these three components provides users with more comprehensive and effective sleep support.

The invention belongs to the technical field of agricultural product preservation, and discloses a lettuce preservation method which comprises the following steps: raw material selection and pretreatment: harvesting fresh lettuce with proper maturity, removing outer leaves and impurities, washing surface floating dust with clear water, and draining for later use; preparation of a melatonin solution: dissolving melatonin in a small amount of methanol, and diluting with distilled water to a target concentration; spraying the picked lettuce with a melatonin aqueous solution, and draining the lettuce subjected to spraying treatment; and storing the drained lettuce in a normal temperature environment of 20-25 DEG C or a refrigeration environment of 4 + / -1 DEG C. The method can prolong the shelf life of the lettuce and maintain the freshness, nutrition and sensory quality of the lettuce.

The invention discloses sleep-aiding yoghurt and a preparation method thereof. Firstly, lactobacillus gasseri and lactobacillus rhamnosus are adopted to ferment poria cocos and lilies, sleep-aiding metabolites are enriched, and lactobacillus gasseri, lactobacillus rhamnosus and metabolites thereof can form metabolite, so that the bottleneck of traditional probiotic application is broken through; a lipidosome embedding technology is combined with fermentation liquid polysaccharide protection and pachymic acid receptor regulation effects, so that the sleep aiding effect of the GABA is enhanced, and the bioavailability and the blood-brain barrier penetrating power of the GABA are improved; and finally, compounding the sleep-aiding functional substances, the cherry pulp and the pectin, covering the bitter taste, constructing a stable system, enhancing the sleep-aiding effect by virtue of melatonin and tryptophan in the cherries, and taking both functionality and palatability into account. The finished product is prepared through the steps of sleep-aiding functional substance preparation, cherry mixed pulp preparation, yoghourt fermentation, blending and after-ripening and the like, the sleep-aiding active ingredients are stable, the flavor is coordinated, and wide application prospects are achieved.

The application discloses a composition containing saffron powder and application thereof in improving sleep, and components of the composition include saffron powder, chamomile powder and green tea extract, and the composition can be any two components compounded in a specific proportion or three components compounded in a specific proportion, and the composition has a significant improvement effect on sleep of mice; when the composition is the combination of the three components, the composition is composed of the saffron powder 0.1-11 parts, the chamomile powder 0.08-8 parts and the green tea extract 0.21-21 parts by weight, and the improvement effect of the three components compounded is more significant and is equivalent to the effect of melatonin. The components of the composition are all derived from food raw materials, are safe and reliable, the preparation process is simple and easy to implement, and the composition is easy to be industrialized and popularized.

Provided herein are methods and compositions for a melatonin-enriched composition of Pistacia vera (Pistachio).

The present disclosure relates to a stem cell-drug carrier including a melatonin-containing drug carrier and a stem cell, and more particularly, to a use of the stem cell for the treatment of intestinal epithelial damage disease and inflammatory bowel disease. According to the present disclosure, it was confirmed that the stem cell-drug carrier continuously released PGE2 and had excellent revival stem cell induction ability. This means that the stem cell-drug carrier of the present disclosure has an excellent intestinal epithelium regeneration effect, and the stem cell-drug carrier of the present disclosure can be used in various fields of treatment of intestinal damage disease and inflammatory bowel disease.

The invention discloses application of a tomato SlEIL2 gene as a negative regulatory factor in preparation of melatonin nutrition fortified tomato fruits and a method of the tomato SlEIL2 gene, the SlEIL2 gene is knocked out through a CRISPR (clustered regularly interspaced short palindromic repeats) technology, the melatonin content of the tomato fruits is remarkably increased, and it is indicated that the SlEIL2 inhibits synthesis of the melatonin of the tomato fruits. Therefore, knockout of the tomato fruit SlEIL2 gene is one of important means for obtaining tomato fruits with high melatonin content, and an effective thought and a reliable scheme are provided for biological research on synthesis of tomato fruit melatonin.

The present invention relates to a powder for reconstitution before use for preparations for injection containing melatonin, at least one soluble excipient and at least one surfactant for the treatment of neonatal cerebral infarction. The present invention also relates to a preparation for injection in the form of a solution obtained by dissolving a powder to be reconstituted comprising melatonin, at least one soluble excipient and at least one surfactant, in a mixture of water and polyalkylene glycol, in which the melatonin is present in quantities of from 3 to 30 mg / ml and the polyalkylene glycol is present in quantities from 5 to 40% of the total volume of the liquid used.

The invention discloses melatonin-chitosan-attapulgite composite gel beads and a preparation method thereof. The preparation method comprises the following steps: S1, treating attapulgite with organic acid to obtain activated attapulgite; s2, dispersing the activated attapulgite into the chitosan acid sol; s3, adding melatonin into the dispersed sol, and mixing in a dark place to obtain colloid; and S4, dropwise adding the colloid into a cross-linking agent, and standing to form gel beads. According to the melatonin-chitosan-attapulgite composite gel bead, the melatonin-chitosan-attapulgite composite gel bead is constructed, the water retention performance of the composite gel bead is excellent, chitosan and attapulgite synergistically improve the water absorption and holding capacity, soil moisture loss is reduced, the water saving effect is remarkable, and the melatonin-chitosan-attapulgite composite gel bead is suitable for agricultural production in water-deficient areas. The composite carrier can slowly release melatonin, prolong the action period of melatonin, regulate and control crop physiology, enhance stress resistance and achieve the dual effects of moisture guarantee and stress resistance regulation and control.

The present application relates to the field of aquaculture technology, and particularly relates to a culture method of bigmouth bass gill explants and application. The present application provides a culture method of bigmouth bass gill explants, which uses explant culture solution to culture the gill explants of bigmouth bass. The explant culture solution is formed by adding fetal bovine serum, fish serum, trinity, D-galactose, L-alanyl-L-glutamine, sodium pyruvate and melatonin into a basic culture medium. In combination with dynamic culture conditions, the natural three-dimensional structure and intercellular connection of the gill tissue can be preserved, and the metabolic activity and functional integrity thereof can be maintained. The present application can solve the technical problem that the prior art cannot provide an ideal research model which can both maintain the structural integrity of the gill tissue and accurately reflect the functional characteristics of the gill tissue. Through the culture method, research models such as ammonia nitrogen stress can be efficiently constructed, thereby providing technical support for the research of bigmouth bass gill tissue physiological mechanism, disease prevention and control, environmental risk assessment and the like.

The present invention relates to a non-therapeutic cosmetic composition for increasing the cellular mitochondrial respiration of keratinocytes underlying the stratum corneum of the epidermis, comprising an active complex: extracts of Melaleuca alternifolia, extracts of Lavandula hybrida, and melatonin. The invention also relates to the use of this non-therapeutic cosmetic composition for increasing the cellular mitochondrial respiration of keratinocytes underlying the stratum corneum of the epidermis.

The invention relates to the technical field of medicines, health care products and functional foods, and particularly discloses an anti-osteoporosis natural product composition based on sleep disorder symptom improvement. The composition comprises 20-40 parts of a component A, 10-50 parts of a component B and 30-40 parts of a component C, the component A comprises 0.1-1.0 part of melatonin, 5-9 parts of lyophilized powder of Yunnan elaeagnus elaphus, 5-10 parts of yam flour and 5-20 parts of millet flour; the component B is prepared from 5 to 25 parts of royal jelly freeze-dried powder, 3 to 24 parts of pollen aqueous extract and 0.001 to 0.05 part of vitamin K2; the component C is prepared from 5 to 10 parts of ganoderma lucidum spore powder, 10 to 25 parts of shrimp shell powder, 10 to 20 parts of blueberry freeze-dried powder and 0.001 to 0.005 part of vitamin D3. The composition is designed by taking improvement of sleep disorder as a key entry point for preventing and treating osteoporosis, and the limitation that only local or single factors of bone metabolism are concerned traditionally is broken through.

The invention discloses an intestinal bacterium-derived melatonin synthetase and an application thereof. Specifically, the melatonin synthetase is selected from one or more of a DDC enzyme, an AANAT enzyme and an AST enzyme, the DDC enzyme comprises an amino acid sequence as shown in SEQ ID NO.2 or an active fragment of the amino acid sequence, or an analogue of which the similarity with the amino acid sequence as shown in SEQ ID NO.2 is more than 80%, and the DDC enzyme comprises an amino acid sequence as shown in SEQ ID NO.2 or an active fragment of the amino acid sequence as shown in SEQ ID NO.2 or an analogue of which the similarity with the amino acid sequence as shown in SEQ ID NO.2 is more than 80%. The AANAT enzyme comprises an amino acid sequence as shown in SEQ ID NO.34 or an active fragment thereof, or an analogue of which the similarity with the amino acid sequence as shown in SEQ ID NO.34 is more than 80%, and the AST enzyme comprises an amino acid sequence as shown in SEQ ID NO.42 or an active fragment thereof, or an analogue of which the similarity with the amino acid sequence as shown in SEQ ID NO.42 is more than 80%. On one hand, the melatonin synthetase can be used for improving the melatonin level of a host and improving related diseases; on the other hand, the method can be used for biosynthesis of melatonin and has a good application prospect.