551 results about "Melatonin" patented technology

The invention belongs to the technical field of food microorganisms, and relates to lactobacillus plantarum and application thereof in preparation of products for relieving insomnia. The preservation number of the lactobacillus plantarum is GDMCC No: 65061. The lactobacillus plantarum can be applied to efficient fermentation of spina date seed juice, the content of GABA, polypeptide, total phenols and total flavonoids in the spina date seed juice is increased, and the content of spinosin, 6 ''-feruloyl spinosin, jujuboside A, jujuboside B and betulinic acid is increased; the reduction of whole brain coefficient and the increase of liver coefficient caused by long-term insomnia are relieved, the bad condition caused by long-term insomnia in a hippocampus DG region in the brain of the mouse is improved, the sleep time of the mouse is prolonged, and the insomnia symptom of the mouse is relieved; the content of inhibitory neurotransmitters 5-hydroxytryptamine, melatonin and gamma-aminobutyric acid in the hypothalamus of the mouse is increased, the content of excitatory neurotransmitter glutamic acid is reduced, and the reduction of organism immunity and oxidative stress of brain tissues caused by insomnia are relieved.

A foam earplug for reception at least partially within the ear canal and including a body portion formed of resilient foam plastic material having a multiple open cell structure and having a size and shape for at least partial reception within the ear canal. A transdermal material having a particular beneficial action, and with an outer layer of the foam earplug infused with the transdermal material so that the benefits of the transdermal material is in transdermal contact with the ear canal. One example is a humectant material and, specifically, a moisturizer / lubricating material, for providing an increase to the lubrication and moisturizing in the ear canal. A second example is where the beneficial action is provided by systemic distribution such as a sleep aid, i.e., Melatonin, so that when the earplugs are worn during sleep, the earplugs will provide a sleep aid to the wearer.

The invention discloses a magnetic biochar-based saline-alkali soil conditioner as well as a preparation method and application thereof, after a biochar material is loaded on ferrous iron, the biochar material is subjected to alkaline oxidation to obtain ferroferric oxide and a porous biochar material in a stable state, and finally activated humic acid, betaine, trehalose and melatonin are combined with magnetic biochar. The magnetic charcoal-based saline-alkali soil conditioner is low in price and easy to obtain and has a good improvement effect on physicochemical properties of saline-alkali soil and promotion of rice growth, and the preparation process is simple and easy to operate.

Sleep-aiding eyewear that prevents light which inhibits melatonin production from reaching the eye is described. The eyewear that is the object of the present disclosure has improved visibility over other methods of cutting off melatonin-inhibiting wavelengths of light, which enables the user to perform tasks, such as reading and typing, uninhibited. This is achieved in some embodiments using a Bragg grating comprising multiple layers of alternating reflective index material as a surface coating on the lens. This may be combined with lens-tinting, or antireflective coatings, in the eyewear. Furthermore, transition lenses which block melatonin-shifting light when there is a suitable external stimulus, such as blue light from a light emitting diode, are described. The presently described filters are also useful for electronic displays and functional light-transmitting materials.

The invention relates to the technical field of exosomes, in particular to an exosome compound preparation with a hair growing effect and a preparation method of the exosome compound preparation. Comprising 12 to 22 parts of ginsenoside Rb1, 15 to 12 parts of biotin tripeptide-15, 8 to 15 parts of diammonium glycyrrhizinate, 50 to 70 parts of fish collagen peptide, 10 to 20 parts of soybean peptide, 0.5 to 4.5 parts of SHH protein, 0.5 to 2.5 parts of SMO inhibitor, 0.5 to 1.5 parts of HhSP protein, 5 to 10 parts of N-acetyl tyrosine, 0.1 to 2 parts of hyaluronic acid, 0.1 to 1 part of melatonin, 0.1 to 1 part of quercetin, 0.1 to 1 part of kaempferol, 0.1 to 1 part of tanshinone, 0.1 to 1 part of biotin and 0.1 to 1 part of vitamin A. The compound preparation is combined with exosomes from various sources, so that the hair papilla cell function is synergistically enhanced, and the damaged hair follicle structure is repaired.

The invention discloses a compound composition for improving sleep disorders, which is characterized by comprising the following components: an inhibitory neurotransmitter component, melatonin and a nano-lipid carrier, the nano-lipid carrier comprises phospholipid and cholesterol, the weight ratio of phospholipid to cholesterol is (1-6): 1, the weight ratio of phospholipid to cholesterol is (1-6): 1, and the weight ratio of melatonin to cholesterol is (1-6): 1. The inhibitory neurotransmitter component is wrapped in the nano-lipid carrier to form lipid nanoparticles, and the weight ratio of the inhibitory neurotransmitter component to the nano-lipid carrier is 1: (1-15). The compound composition can be prepared into a compound oral mucosa preparation, can be rapidly disintegrated when encountering saliva in the oral cavity, is good in patient compliance and small in side effect, all the components play a synergistic effect from different mechanisms through multiple target points, and the compound oral mucosa preparation can improve the sleep quality, prolong the sleep time and shorten the sleep latency; and patients with sleep disorders are helped to improve insomnia from the pathologic root.

The invention discloses nano-composite hydrogel based on active oxygen removal as well as a preparation method and application of the nano-composite hydrogel, and relates to the technical field of biomedicine. The nano composite hydrogel based on active oxygen removal comprises a three-dimensional network skeleton structure formed by cross-linking oxidized chondroitin sulfate and carboxymethyl chitosan, and melatonin-loaded mesoporous polydopamine nanoparticles are encapsulated in the three-dimensional network skeleton structure. According to the invention, a nano material is combined with a three-dimensional network skeleton structure of the hydrogel, a novel nano composite hydrogel is developed, and the nano composite hydrogel has self-healing ability, good biocompatibility, good mechanical properties and long-acting drug release ability; meanwhile, the melatonin is loaded into the nano-composite hydrogel to develop a medicine compound, the medicine activity and the treatment effect are better than those of the melatonin, inflammation and oxidative stress can be relieved more effectively, and symptoms can be improved more effectively.

The invention provides a method for improving germination of rice seeds under salt stress and application, and relates to the field of crop seed germination, in particular to application of melatonin and vitamin C. The use concentration of the melatonin is 10 micromol / L, 50 micromol / L, 100 micromol / L, 200 micromol / L and 300 micromol / L, and the use concentration of the vitamin C is 5 micromol / L, 10 micromol / L, 20 micromol / L, 30 micromol / L and 50 micromol / L. The combined concentration of the melatonin and the vitamin C is 200 [mu] mol / L and 30 [mu] mol / L, the germination rate and the germination potential are significantly improved after the melatonin and the vitamin C are applied for treatment under salt stress, the germination effect of the rice seeds is optimal after the rice seeds are treated for 7 days by the 200 [mu] mol / L melatonin and the 30 [mu] mol / L vitamin C, the inhibition effect of salt stress on the rice seeds can be significantly relieved, and the salt stress resistance of the rice is improved. Therefore, the invention provides a theoretical basis for researching the mechanism that melatonin and vitamin C improve rice seed germination under salt stress, and provides practical guidance for research on promotion of plant salt tolerance at the same time.

The invention provides a feed domesticated siniperca chuatsi sperm low-temperature preservation and ultralow-temperature preservation method, which comprises the following steps: firstly, providing a siniperca chuatsi sperm low-temperature preservation diluent which comprises Hanks liquid, sodium chloride, soya bean lecithin, vitamin E, curcumin, melatonin and SOD (superoxide dismutase); and adding an anti-freezing protective agent into the low-temperature preservation diluent to obtain the ultralow-temperature preservation anti-freezing liquid for the siniperca chuatsi sperms. The siniperca chuatsi semen and the low-temperature preservation diluent are uniformly mixed, so that low-temperature preservation can be realized; the siniperca chuatsi semen is uniformly mixed with the cryopreservation anti-freezing liquid, so that cryopreservation can be realized. The siniperca chuatsi sperm low-temperature preservation diluent is preferably selected, low-temperature preservation of the siniperca chuatsi sperms can be achieved, ultralow-temperature preservation can be conducted after an anti-freezing protective agent is added, the persistence of the preservation time of the siniperca chuatsi sperms can be guaranteed, the protection effect on the sperms is good, and the activation rate and activity of the unfrozen sperms are high.

The invention discloses a self-adaptive display definition optimization system and method based on time variation, and the system comprises a time detection module which is used for obtaining the information of a current time period; the ambient light sensor is used for detecting ambient light intensity and color temperature; the display parameter adjusting module is used for dynamically adjusting the resolution, sharpness, color temperature, brightness and blue light proportion of the display according to the time period and the ambient light data; the user habit learning module is used for analyzing user manual adjustment records and optimizing default parameters; the eye protection mode module is used for triggering protective adjustment of display parameters according to the continuous use duration; according to the invention, the resolution and sharpness can be automatically adjusted according to different time periods, and the definition and the visual comfort are balanced; blue light radiation is intelligently reduced at night, and interference on melatonin secretion is reduced; unnecessary display parameters are automatically reduced according to ambient light and time, and the endurance of equipment is prolonged; and a customized display mode is provided by learning user habits.

The invention belongs to the technical field of biological medicine, and particularly relates to a stem cell preparation based on immunoregulation and a preparation method and application thereof. The stem cell preparation based on immunoregulation is prepared from the following raw materials in parts by weight: 5 to 40 parts of freeze-dried powder, 40 to 120 parts of gastrodia elata polysaccharide and 2 to 6 parts of melatonin, the freeze-dried powder is obtained by mixing and freeze-drying a stem cell culture solution and curcumin lipidosome; the volume ratio of the stem cell culture solution to the curcumin liposome is 1: (10-32); the protein concentration in the stem cell culture solution is 1.2 mg / mL to 1.6 mg / mL. The stem cell preparation provided by the invention can utilize the immune regulation effect of the mesenchymal stem cells to realize the immune repair of adipose tissues.

The invention discloses a two-dimensional organic / inorganic heterojunction photoelectric nerve component and a preparation method thereof, and belongs to the technical field of photoelectric devices. According to the invention, a TMDC material MoS2 is transferred to a substrate through a mechanical stripping method, then a film electrode is plated on a MoS2 layer through an electron beam lithography method, and finally a single-layer Me-PTCDI crystal is epitaxially grown on the MoS2 layer through a back-off method to form the heterojunction. Due to the formation of the MoS2 / Me-PTCDI heterojunction, the photoelectric nerve device has excellent photosensitivity and light adaptability, and can simulate reactions of retinal essential photosensitive ganglion cells under different illumination; the device can make current change under the stimulation of light pulses so as to simulate the generation and action of human melatonin, and can realize visual adaptation in bright and dark environments under the regulation and control of grid voltage, and the device has a wide application prospect in the field of automatic driving due to the corresponding characteristics.

The invention discloses a sleep-aiding traditional Chinese medicine composition and a preparation method and application thereof. The sleep-aiding traditional Chinese medicine composition is prepared from 50-80 parts of spina date seeds, 50-80 parts of polygala tenuifolia, 50-80 parts of lilies, 50-80 parts of poria cocos, 30-50 parts of red dates, 30-50 parts of lotus seeds, 30-50 parts of longan aril, 30-50 parts of mulberries, 20-30 parts of seaweed polyphenols and 20-30 parts of gamma-aminobutyric acid. Pharmacodynamic tests show that the composition can remarkably shorten the sleep latency time of mice, prolong the total sleep time and remarkably improve the levels of 5-hydroxytryptamine, melatonin and gamma-aminobutyric acid in serum of the mice, so that the composition has an obvious sleep aiding effect through scientific proportioning and synergistic interaction of the raw materials.

The invention belongs to the technical field of sleep-aiding gels, and discloses a herbal sleep-aiding gel and a preparation process thereof, the gel comprises the following components: deionized water, propylene glycol, glycerol, carbomer 980 and triethanolamine; the mask is prepared from, by weight, 1.8% of lavender essential oil, chamomile extract, bitter orange flower essential oil, gamma-aminobutyric acid, melatonin, spina date seed extract, xylaria nigripes extract and phospholipid wall materials. Sodium chloride and azone; camphor and borneol; eDTA (Ethylene Diamine Tetraacetic Acid) disodium, phenoxyethanol and ethylhexylglycerin. The traditional Chinese medicine composition is suitable for people with sleep disorders such as long-term insomnia, difficulty in falling asleep, insomnia and dreaminess, easy awakening due to light sleep, restlessness and depression and the like; through the high transdermal absorption rate of a nano micro-capsule technology, in combination with a colloid double-electrode-layer structure, capacitance-like micro-current is generated, in cooperation with lavender-chamomile-bitter orange flower and other herbal essence and GABA and melatonin active ingredients, the sleep center is adjusted in a multi-channel mode, and the physical sleep-aiding scheme is a new physical sleep-aiding scheme for safely replacing a sleeping drug.

The method comprises the following steps: fixing with a pre-fixing solution, wrapping and forming with a solid agent, fixing with a fixing solution, embedding with an embedding agent, decomposing melanin with a melanin decomposing solution, pre-decoloring with a pre-decoloring solution, decoloring with a decoloring solution, and eluting with a rinsing solution. The invention provides a melanin removing method capable of effectively avoiding retina RPE layer cell exfoliation, protein denaturation and nucleic acid degradation. The method mainly comprises the following steps: keeping the original state of tissue cells by adopting a pre-fixing solution, keeping the complete tissue structure by adopting a solid agent, decomposing melanin particles by adopting a melanin decomposing solution composed of various extracts, separating melanin by adopting a pre-decoloring solution and a decoloring solution, eluting the melanin decomposed on the surface of the tissue by adopting a rinsing solution, and the like. On the premise that the retina tissue structure is intact and protein, nucleic acid and cell structures on the surface of the tissue section are not damaged, melanin particles in the tissue section are effectively decomposed, the biological activity of various organelles on the surface of the section is kept, and the requirements of subsequent chemical staining of various tissues are met.

The invention discloses a method for creating melatonin-rich soybeans by utilizing synthetic regulatory elements and application of the melatonin-rich soybeans. According to the invention, a soybean standard line Williams 82 (W82) is used as a receptor, melatonin pathway genes COMT and SNAT are optimized through codons to synthesize regulation elements to efficiently and accurately activate expression of COMT and SNAT, and specific accumulation in soybean seeds is realized, so that a new soybean material rich in melatonin in the seeds is created. Compared with a control receptor, the melatonin content of the modified soybean is increased by more than 30 times. The method for efficiently producing the melatonin by synthesizing the regulatory element has an important value for increasing the content of the melatonin, provides a new thought and tool for improving the quality of soybeans and crops, is beneficial for supplementing high-value active substances in daily diet, and has a wide application prospect. The method has strong application potential in the aspect of assisting biosynthesis of plant-derived natural products.

The invention is suitable for the technical field of health care, and provides a health-care food formula for improving sleep. Comprising the following raw materials in parts by weight: 150-250 parts of whole-grain oatmeal, 100-200 parts of germinated brown rice powder, 60-80 parts of pumpkin seeds, 50-60 parts of black sesame, 40-50 parts of flaxseeds, 62-70 parts of dried sour cherries, 55-60 parts of dried figs, 45-52 parts of dried bananas, 20-25 parts of chamomile flower powder, 18-20 parts of ground cinnamon, 50-60 parts of fructus cannabis protein powder, 70-80 parts of millet powder, 35-40 parts of hericium erinaceus powder and 18-20 parts of ganoderma lucidum spore powder. The beverage is prepared from 48-50 parts of fructo-oligosaccharide, 35-40 parts of date palm paste, 25-30 parts of beer yeast powder, 28-30 parts of walnut oil, 15-20 parts of semen ziziphi spinosae and 10-15 parts of semen boitae. Various nutritional foods are adopted, so that the problem of difficulty in falling asleep caused by insufficient secretion of melatonin can be solved, and anxiety, light sleep caused by excessive activity of brain waves and the like can be relieved.

The invention discloses an oral sleep-aiding composition containing ergothioneine and a preparation method of the oral sleep-aiding composition, and relates to the technical field of sleep-aiding compositions. The oral sleep-aiding composition containing the ergothioneine is prepared from the following components in parts by mass: 0.01 to 3 parts of the ergothioneine, 0.1 to 10 parts of melatonin, 10 to 40 parts of glycine, 2 to 15 parts of magnesium salt, 30 to 70 parts of a diluent, 1 to 10 parts of an adhesive, 0.5 to 5 parts of a lubricant, 2 to 10 parts of a disintegrating agent and 0.1 to 0.5 part of an antioxidant preservative. Through the synergistic compatibility of ergothioneine, melatonin, glycine and magnesium salt, a multi-dimensional action network of oxidation resistance, neuromodulation and receptor inhibition is formed, the limitation of single-component sleep aiding is broken through, and the sleeping efficiency and the sleep maintenance capability are remarkably improved. Based on the scientific proportion of endogenous substances (ergothioneine and glycine), the metabolic burden of traditional sleep-aiding components is avoided, and in combination with the design of a sustained-release preparation, the dose-dependent side effects are reduced, and the safety of long-term taking is improved.

The invention discloses a melatonin rapidly disintegrating oral tablet and a preparation method thereof, and the melatonin rapidly disintegrating oral tablet is prepared from the following raw materials in parts by weight: 40 to 55 parts of mannitol, 3 to 6 parts of cross-linked povidone, 0.5 to 2.0 parts of povidone, 2 to 6 parts of melatonin, 30 to 45 parts of filler and 0.3 to 1.5 parts of lubricant. Wherein the mannitol is beta-crystal form mannitol. According to the invention, mannitol of a limited type is matched with raw materials such as polyvinylpolypyrrolidone, and meanwhile, a specific mixing and tabletting process is adopted, so that the tablet can be rapidly disintegrated in an oral cavity while the hardness of the tablet is maintained, the bioavailability is high, and the requirements of the health food field on taking convenience, effectiveness and industrial production can be met.

The invention belongs to the technical field of functional preparations, and discloses a composite emulsion for improving insomnia and a preparation method thereof. The composite emulsion is prepared from the following components in percentage by volume: 70 to 95 percent of modified fucoidin solution with the concentration of 0.5 to 3 mg / mL, and the balance of melatonin-containing medium chain triglyceride, the content of the melatonin in the composite emulsion is 0.02 wt%. The compound emulsion is strong in stability and high in bioavailability, and can effectively resist an acid environment in a stomach and protect melatonin from being delivered to intestinal tracts. Through verification, the average core sleep duration of a subject taking the compound emulsion is increased by 21.05% compared with that of the subject taking melatonin only; the average waking time is shortened by 40% compared with that of melatonin, and the effect is remarkable.

The invention discloses a caffeic acid-O-methyltransferase 2 function acquired mutant, a recombinant expression vector and application thereof, and belongs to the technical field of crop biological breeding. It is found for the first time that after the 344 amino acid of tomato caffeic acid-O-methyltransferase 2 is changed into tryptophan (W) from cysteine (C), the melatonin content of tomatoes can be increased, the tomatoes can be endowed with broad-spectrum resistance to abiotic and biological stress, and the tomato caffeic acid-O-methyltransferase 2 can serve as a key target for tomato resistance breeding.

The invention relates to responsive hydrogel loaded with bionic nano-vesicles as well as a preparation method and application of the responsive hydrogel, and belongs to the technical field of medical hydrogel. The method comprises the steps that odontogenic mesenchymal stem cells are stimulated by a chemical vesicle stimulant and then mixed with melatonin mother liquor for extrusion, the bionic nano vesicles are obtained, the chemical vesicle stimulant contains a buffer solution, a dithiothreitol solution and a paraformaldehyde solution, and the buffer solution contains 8.5-9 g / L of sodium chloride, 0.2-0.24 g / L of calcium dichloride and 2.3-2.4 g / L of 4-hydroxyethyl piperazine ethanesulfonic acid; 6-arm-PEG-pp-NH2 coupled with polypeptide capable of being cut by matrix metalloproteinase, six-arm polyethylene glycol succinimide glutarate and the bionic nano-vesicle are prepared into a first precursor solution and a second precursor solution respectively, the first precursor solution and the second precursor solution are mixed and subjected to standing to form gel, and the responsive hydrogel is prepared. According to the invention, the responsive hydrogel capable of obviously enhancing bone regeneration capacity is obtained.

The invention relates to the technical field of plant stress regulation and medicinal plant cultivation. A salt stress damage repair method for cutting seedlings of aromatic medicinal plants comprises the following steps: after asexual cutting seedlings of the aromatic medicinal plants suffer from salt stress, spraying a melatonin aqueous solution with the concentration of 50-200 [mu] mol.L to the leaves of the seedlings in a repair window period of 3-6 days after the salt stress occurs, spraying 1-2 times a day, continuously spraying for 2-3 days, and continuously spraying the melatonin aqueous solution with the concentration of 50-200 [mu] mol.L to the leaves of the seedlings in the repair window period of 3-6 days after the salt stress occurs. Melatonin is used for repairing salt damage of the asexual cuttage seedlings of the aromatic medicinal plants. The melatonin can be accurately applied before the salt damage critical point to repair the physiological damage of the cutting seedlings of the aromatic medicinal plants, and the salt damage influence of the cutting seedlings of the aromatic medicinal plants is greatly reduced; the problems that cutting seedlings of the aromatic medicinal plants are prone to salt damage under salt stress, the survival rate is low, and the content of main medicinal components is reduced are solved, and the dual effects of seedling physiological restoration and medicinal component content stabilization can be achieved.

The invention discloses a melatonin detection method which comprises the following steps: S1, coupling an anti-melatonin antibody with a PE fluorescent label with carboxyl fluorescent magnetic beads to construct antibody coupled magnetic beads MBs (at) Ab; s2, preparing a melatonin analogue FITC-MT by using the 5-MT and the FITC-NHS (N-hydroxysuccinimide); s3, preparing MBs (at) Ab and FITC-MT into a reaction system, reacting with melatonin standard substances with different concentrations or a to-be-detected sample, detecting by using a flow cytometer, processing a detection result of the flow cytometer by using a machine learning algorithm, and calculating the melatonin concentration in the to-be-detected sample. MT in a to-be-detected sample can compete for FITC-MT combined with MBs (at) Ab in a short time to form a stable reaction system, the multi-dimensional features of MBs (at) Ab are subjected to flow detection to directly remove impurities, agglomeration and the like in MBs (at) Ab, different fluorescence features of an optimal magnetic bead group in the system are searched and analyzed in combination with a machine learning algorithm, and the content of MT can be accurately detected; the reaction system constructed by the invention is simple to operate and extremely low in cost, and meanwhile, the result accuracy can be ensured.

The invention discloses a method for promoting biomass accumulation of haematococcus pluvialis, which comprises the following steps: adding salicylic acid, melatonin and alpha-ketoglutaric acid into a basic culture medium, and replacing ferric trichloride with ferric citrate as an iron source to obtain an improved BG-11 culture medium; the method comprises the following steps: inoculating a haematococcus pluvialis seed solution into a culture bottle containing an improved culture medium, carrying out illumination treatment on the first day to the second day by adopting pulsed red light with the wavelength of 620 nm in the early stage of a green stage, carrying out illumination treatment on the third day to the fifth day by adopting red light with the wavelength of 660 nm in the middle stage of the green stage, and carrying out illumination treatment on the sixth day to the seventh day by adopting pulsed red light with the wavelength of 680 nm in the later stage of the green stage. Through a synergistic scheme of the improved BG-11 culture medium, staged red light treatment and dynamic environment regulation and control, the problems of low light energy utilization rate and insufficient stress resistance in the existing haematococcus pluvialis culture process are solved, high efficiency and stability of haematococcus pluvialis biomass accumulation are realized, the biomass can reach 7.0 * 10 < 5 > cells / mL in the later period of the green stage, and the yield of the haematococcus pluvialis can reach 7.0 * 10 < 5 > cells / mL in the later period of the green stage. And the survival rate of haematococcus pluvialis cells reaches 95%.

The invention provides a microbial marker and a prediction model for schizophrenia with social anxiety disorder. The microbial marker is a Bacteroides microorganism in saliva. The relevance between the relative abundance of Bacteroides microorganisms in saliva and social anxiety disorder is found for the first time. A Nomogram clinical prediction mode is formed by taking the specific microbial marker as a microbial marker in combination with a children bad experience scale and 6-hydroxymelatonin and 5-hydroxyindoleacetic acid in serum, so that objective biological indexes can be provided for early diagnosis and monitoring of whether schizophrenia is accompanied by social anxiety disorder or not; errors of subjective evaluation are reduced; moreover, the oral saliva sample has the obvious advantage of being easy to collect for psychiatric patients, the working difficulty of medical staff can be greatly reduced, and revolutionary changes of prediction, diagnosis and treatment of schizophrenia accompanied with social anxiety disorder are expected to be brought.

The invention belongs to the technical field of animal gene engineering, and particularly relates to a down producing goat lncRNA, an interference vector and application of the interference vector to regulation and control of down producing goat hair follicle development, the down producing goat lncRNA is lncRNA TCONS-00237214 and is used for regulating and controlling down producing goat hair follicle development; the nucleotide sequence of the lncRNA TCONS-00237214 is as shown in SEQ ID NO. 1 (sequence identifier number 1). According to the invention, the hair papilla cells are selected for subsequent experimental verification, and the cell cycle, cell proliferation and cell migration of the hair papilla cells are detected by constructing an interference lncRNA TCONS-00237214 stable transformation hair papilla cell line. Results of the invention show that melatonin can regulate and control expression of lncRNA TCONS-00237214, participate in cell cycle transformation, promote proliferation and migration of hair papilla cells and inhibit apoptosis of the hair papilla cells, thereby affecting generation and development of hair follicles.

The invention relates to the technical field of plant genetic engineering, in particular to a medicago sativa MsSNAT1 gene and application of the medicago sativa MsSNAT1 gene in regulation and control of plant salt tolerance. The nucleotide sequence of the medicago sativa MsSNAT1 gene is as shown in SEQ ID NO. 1. Experimental results show that overexpression of the MsSNAT1 gene enhances the stress resistance of medicago sativa. The overexpression of the MsSNAT1 gene promotes the increase of the content of endogenous melatonin and increases the expression of the melatonin synthetase gene under salt stress. Compared with a wild type, the antioxidant enzyme activity and osmoregulation substance increase trend of the transgenic line under salt and drought stress is more obvious, the MDA content is lower, and the negative influence is smaller. In addition, the content of endogenous melatonin in a transgenic line is increased, and the endogenous melatonin and exogenous ABA act together, so that the oxidation resistance and osmotic regulation capacity of the plant are enhanced, and the stress resistance of the plant is remarkably improved.

The invention relates to the technical field of veterinary drug preparation production, and discloses a melatonin implant, the melatonin implant comprises an embedded core and a coating, the coating is sprayed on the surface layer of the embedded core, the embedded core comprises the following components by mass: 65-75% of melatonin, 8-12% of a carrier, 10-15% of a lubricant, and 7-9% of a first plasticizer; the coating comprises the following components: a film-forming agent, a second plasticizer and a solvent, the mass ratio of the film-forming agent to the second plasticizer is (1.5-2.5): 1, and the ratio of the mass of the film-forming agent to the volume of the solvent is (2.5-3.5): 100 (g / mL). The implant has large drug loading capacity and can be slowly released for a long time, and only 13% of the implant can be released in vitro for 28 days. The melatonin implant can be prepared by adopting a hot melt extrusion and coating method, and the preparation method has the advantages of simple process, continuous production, short and stable production period, less material loss, better drug content uniformity and high technical yield, and is beneficial to expanded production.

The invention relates to a composition for improving levels of endogenous gamma-aminobutyric acid and melatonin and application of the composition. The composition comprises a pseudo-ginseng stem and leaf compound, gamma-aminobutyric acid, L-theanine and a liquorice extract, the pseudo-ginseng stem and leaf compound is a product obtained by alcohol extraction of pseudo-ginseng stems and leaves, gastrodia elata, poria cocos and spina date seeds as raw materials. The pseudo-ginseng stem and leaf compound is developed firstly, the pseudo-ginseng stem and leaf compound is obtained by taking four specific medicinal and edible materials including pseudo-ginseng stems and leaves, gastrodia elata, poria cocos and spina date seeds as raw materials through alcohol extraction, and the four raw materials are matched with one another and have a potential mutual promotion effect in the aspect of improving endogenous gamma-aminobutyric acid and melatonin. The pseudo-ginseng stem and leaf compound is creatively combined with gamma-aminobutyric acid, L-theanine and a licorice extract for use, and the four components cooperate with each other, so that the effect of improving the levels of endogenous gamma-aminobutyric acid and endogenous melatonin is further improved.