318 results about "Astragalus polysaccharide" patented technology

The invention discloses astragalus polysaccharide processed by different processing technologies and application thereof, self-weight polysaccharides of RAPSs, SAPSs, WAPSs and HAPSs are extracted from crude products and processed products obtained by salt processing, wine processing and honey processing, and the influence of different processing methods on the structure and bidirectional immunomodulatory activity of the astragalus polysaccharide is discussed. Results show that different processing methods change the properties and structural characteristics of the astragalus polysaccharide so as to influence the bidirectional immunomodulatory activity of the astragalus polysaccharide; the extraction rate, chemical composition, molecular weight, monosaccharide composition, chain conformation and surface morphology, Zeta potential and particle size, immunity enhancement and anti-inflammatory activity and the like of the four polysaccharides are remarkably different, but the four polysaccharides have the same typical crystal interior, main glycoside residues and O-glucosidic bonds. In a test concentration range, the honey-processed polysaccharide has a relatively strong immunity enhancing capability, and the wine-processed polysaccharide has a relatively strong anti-inflammatory capability.

The invention relates to the technical field of astragalus polysaccharide, in particular to an extraction method of astragalus polysaccharide. An extraction method of astragalus polysaccharide comprises the following steps: primary water extraction, secondary water extraction, vacuum concentration, primary alcohol precipitation, secondary alcohol precipitation and purification, specifically, the primary water extraction comprises the following steps: soaking astragalus with a weakly alkaline solution, heating until boiling, extracting, and filtering to separate primary precipitate and primary extract; the weakly alkaline solution is used in the primary water extraction step, so that cell walls are damaged, the solubility of the polysaccharide is improved, the release of the polysaccharide is promoted, and the extraction rate of the astragalus polysaccharide is remarkably improved.

The invention provides a microecological preparation with an immunological enhancement function and application of the microecological preparation in antibiotic-free breeding of livestock and poultry, and relates to the technical field of agricultural biology. The microecological preparation with the immunological enhancement function comprises a probiotic component, a prebiotics component and an immunological enhancement component, and the probiotic component, the prebiotics component and the immunological enhancement component are respectively composed of lactic acid bacteria, bacillus, clostridium butyricum, fructo-oligosaccharide, yeast beta-glucan, astragalus polysaccharide and chitosan oligosaccharide. Wherein the lactic acid bacteria accounts for 30%-40%, the bacillus accounts for 20%-30%, the clostridium butyricum accounts for 10%-20%, the fructo-oligosaccharide accounts for 5%-10%, the yeast beta-glucan accounts for 3%-8%, the astragalus polysaccharide accounts for 2%-5%, and the chitosan The probiotics can enhance immunity and regulate intestinal tracts at the same time, the effect is improved by 40% or above compared with common probiotics, animals suffer less illness and grow fast, flora can resist high temperature and gastric acid through a special protection technology, the loss of viable bacteria is less than 5% after the probiotics are stored for 3 months, and the probiotics are stable by two times compared with conventional products.

The invention relates to bone paste for repairing bone wounds and a preparation method of the bone paste, and belongs to the technical field of biomedical material manufacturing. The bone paste is prepared from nanoscale calcium-phosphorus biphase ceramic, biomimetic mineralized collagen fiber, biodegradable polymer, modified hyaluronic acid, beta-cyclodextrin coated recombinant human bone morphogenetic protein-7, an angiogenesis accelerant and an immunoregulation additive according to a certain proportion. The nano-scale calcium-phosphorus dual-phase ceramic is hydroxyapatite and beta-tricalcium phosphate dual-phase ceramic; the biomimetic mineralized collagenous fiber is prepared by mixing type I collagen freeze-dried fiber with hydroxypropyl chitosan and performing mineralization; the modified hyaluronic acid is hyaluronic acid-sulfydryl and hyaluronic acid-hydrazide; the angiogenesis accelerant is a polylactic acid sustained release microsphere loaded with tanshinone and hydroxyl polyethylene glycol biotin; the immunoregulation additive is disodium glycyrrhizinate, lentinan and astragalus polysaccharide. The bone paste can effectively improve the bone induction capacity, promote vascularization generation and reduce the immunogenicity risk.

The invention belongs to the technical field of agriculture, and particularly relates to application of an astragalus extract containing astragalus polysaccharide in promoting plant growth. Purified astragalus polysaccharide is proved to have good growth regulation and stress resistance enhancement functions on plants, but the extraction and purification process of the polysaccharide is high in cost and low in efficiency, the requirement of large-scale production is difficult to meet, and the application of the astragalus polysaccharide in agriculture is limited. The invention provides an application of an astragalus extract containing astragalus polysaccharide, and particularly relates to an application of the astragalus extract as a plant growth promoter for promoting plant growth and improving stress resistance of plants, reducing the use concentration of the astragalus polysaccharide, promoting the application of the astragalus polysaccharide in agriculture, and providing a new choice for regulating and controlling plant growth in agriculture.

This application relates to the technical field of astragalus polysaccharides, and specifically relates to a method for extracting astragalus polysaccharides. A method for extracting astragalus polysaccharides includes the following steps: primary water extraction, secondary water extraction, vacuum concentration, primary alcohol precipitation, secondary alcohol precipitation, and purification. Among them, in the primary water extraction: soak astragalus with a weak alkaline solution, then heat to boiling and carry out extraction, and filter to separate the primary precipitate and the primary extract. By using a weak alkaline solution in the primary water extraction step, this application destroys cell walls, increases the solubility of polysaccharides, and promotes the release of polysaccharides, significantly improving the extraction rate of astragalus polysaccharides.

The invention discloses an efficient extraction method of large-molecular-weight astragalus polysaccharide, which belongs to the technical field of plant extraction, and comprises the following steps: cleaning astragalus, filtering, and crushing; the preparation method comprises the following steps: mixing a beta-1, 3-glucanase aqueous solution and trehalose ethylene diamine, and stirring for 10-30 minutes to obtain an enzyme protection system; soaking the radix astragali powder in a citric acid solution, adding an enzyme protection system, applying a bipolar pulsed electric field, and reacting at 40-60 DEG C for 30-40 minutes; adjusting the concentration of the ethanol to 40-50%, standing, centrifuging and collecting crude astragalus polysaccharide; purifying, decolorizing, concentrating, freeze-drying and crushing the crude astragalus polysaccharide to obtain refined astragalus polysaccharide; the mass ratio of the trehalose ethylene diamine to the beta-1, 3-glucanase is (15: 1) to (30: 1). The enzyme protection system maintains the activity of the enzyme, the enzyme and the pulsed electric field act synergistically, the extraction time is shortened, and the extraction rate is increased while the astragalus polysaccharide with large molecular weight is obtained.

The invention discloses a multifunctional coating with a compact-porous double-layer structure on the surface of magnesium alloy and a preparation method, and belongs to the technical field of biological materials. According to the multifunctional coating, magnesium alloy serves as a substrate, a compact layer and a porous layer are sequentially stacked from the substrate to the outside, and the compact layer is composed of two-dimensional transition metal carbide / nitride, calcium silicate nanowires and a positively charged polymer. The porous layer is composed of copper-containing complex / biopolymer composite fibers, mineralized silk fibers loaded with bioactive metal ions and astragalus polysaccharide on the surface, and natural polymers. According to the multifunctional coating with the compact-porous double-layer structure prepared on the surface of the magnesium alloy, the porous layer on the surface layer has a unique porous structure, growth and proliferation of osteoblasts can be well promoted, meanwhile, the multifunctional coating has the characteristics of pressure resistance, bacteria resistance and oxidation resistance, the compact layer on the bottom layer can improve the corrosion resistance of the magnesium alloy, and the service life of the magnesium alloy is prolonged. The coating is particularly suitable for surface coating of magnesium alloy implant materials and can be used in the field of medical instruments.

The invention discloses a holothurian culture feed additive and a preparation method thereof, and belongs to the technical field of aquaculture. According to the technical scheme, the holothurian culture feed additive comprises the following components in percentage by mass: 45%-55% of fish meal, 2%-3% of butyric acid bacteria, 25%-30% of compound probiotics, 2%-4% of a Chinese herbal medicine extract, 8%-12% of a fermentation substrate, 10%-15% of seaweed meal, 0.5%-1% of vitamin E, 0.5%-1% of selenium yeast and the balance of a carrier, the compound probiotics are composed of lactic acid bacteria, bacillus and photosynthetic bacteria according to the mass ratio of 2: 1: 1; the Chinese herbal medicine extract is a mixture of astragalus polysaccharide and an asarum extract in a mass ratio of 3: 1; through the synergistic effect of the fish meal, the compound probiotics, the Chinese herbal medicine extract and other components, nutrition balance and intestinal flora regulation and control are achieved, the problems of slow growth, intestinal diseases and water quality pollution caused by traditional feed are effectively solved, and the sea cucumber feed has the advantages that the survival rate of sea cucumbers is increased, and the culture benefits are increased.

The invention relates to an anti-fatigue refreshing functional chewing gum containing natural active ingredients, which relates to the technical field of food, and comprises a gum base, a natural sweetening agent and an active ingredient mixture, the mustard extract and coffee are innovatively combined to form a dual-channel nerve activation mechanism, the mustard extract realizes instant refreshing through a targeted oral trigeminal nerve TRPA1 ion channel, caffeine maintains continuous excitation through antagonism of a central adenosine receptor, and the space-time cooperation of the mustard extract and the coffee breaks through the limitation of a single path. Meanwhile, a rhodiola rosea extract, a sea-buckthorn extract, an American ginseng extract and astragalus polysaccharide are compounded, so that a triple anti-fatigue physiological barrier with functions of nerve regulation, oxidative stress inhibition and energy metabolism optimization is formed. In the preparation process, a microcapsule embedding technology is adopted to control slow release of mustard active ingredients, menthol flavor is combined to synergistically trigger TRP channel dual activation, and unification of efficacy and palatability is achieved.

The invention provides a feed additive capable of improving immunity of pet cats as well as a preparation method and application of the feed additive, and belongs to the technical field of animal feed additives. The feed additive comprises probiotics and a traditional Chinese medicine extract, the probiotics comprise bifidobacterium animalis, combined lactobacillus salivarius and saccharomyces cerevisiae boulardii, and the traditional Chinese medicine extract comprises astragalus polysaccharide, curcumin and resveratrol. The probiotics directly enhance intestinal immunity through colonization resistance and metabolites, the traditional Chinese medicine components form internal and external dual defense through system immune activation, immunity-flora dual regulation is achieved, the probiotics and the traditional Chinese medicine extract form a symbiotic system of increasing the drug effect through bacteria and protecting the bacteria through drugs, and 1 + 1gt is achieved; and 2, the immunity improving effect is achieved.

The invention discloses a feed containing astragalus polysaccharide and a preparation method thereof, and belongs to the field of feeds. The astragalus polysaccharide is added into the basic feed, so that the adverse effect of heat stress on the female rabbits can be effectively relieved, the conception rate of the female rabbits, the number of live litters and the average weight of the live litters can be increased, and the breeding economic benefits are improved while the reproductive performance is optimized and the growth of the young rabbits is promoted; the prepared feed can effectively improve the serum hormone level of animals and reduce the inflammatory factor level, meanwhile, the diversity of animal intestinal microorganisms under heat stress is effectively improved, a scientific basis is provided for precise feeding management of domestic rabbits in summer, and meanwhile, an important reference is provided for application of astragalus polysaccharide in the field of livestock and poultry anti-stress.

The invention discloses an IV-type collagen detection kit and a preparation method thereof, and relates to the technical field of in vitro diagnos.The kit comprises a reagent R1 and a reagent R2, and the volume ratio of the reagent R1 to the reagent R2 is 3: 1; through an elaborately designed reagent formula, especially the accurate proportion of all components in the reagent R1, such as ammonium chloride, sodium azide and Brij-35, the detection sensitivity and accuracy are improved, the stability and repeatability of the kit are also ensured, and particularly, the natural plant extract astragalus polysaccharide and the protease K inhibitor are introduced into the reagent R1 as key components, so that the detection sensitivity and accuracy are improved, and the detection sensitivity and repeatability are improved. According to the present invention, the unique preparation method and the activity promotion effect on the IV-type collagen hydrolase significantly enhance the biological activity of the kit, such that the detection result is reliable, and the two latex particles coated with the paired mouse monoclonal antibodies adopted in the reagent R2 have high specificity and affinity, such that the kit can be used for the detection of the IV-type collagen hydrolase. And the detection accuracy and sensitivity are further improved.

The invention provides application of enhanced mesenchymal stem cells and exosomes in chronic nephritis and renal failure, and belongs to the technical field of biological medicine manufacturing. Through composite pretreatment of salidroside and astragalus polysaccharide, the treatment potential of exosomes secreted by the mesenchymal stem cells is remarkably enhanced, a new thought is provided for intervention of the chronic kidney diseases, and various pathologies of the chronic kidney diseases are covered. In addition, a 3D microenvironment constructed by fibrin gel is combined to simulate an in-vivo extracellular matrix, the natural growth state of the stem cells is better fitted, directional differentiation and function regulation of the stem cells are promoted, and therefore the biological activity and targeting performance of the exosome are improved.

The invention relates to a beauty and skin care enzyme and a preparation method thereof. According to the invention, fruits and traditional Chinese medicines are used as main fermentation raw materials, the fruits and the traditional Chinese medicines are crushed and mixed with fermentation strains, and active substances in each raw material are effectively retained through two-stage fermentation, filtration separation and freeze-drying treatment, SOD and sea buckthorn flavone contained in sea buckthorn fruits have the skin care effects of resisting oxidation and promoting collagen synthesis, and the sea buckthorn fruits have the skin care effects of resisting oxidation and promoting collagen synthesis. Salidroside contained in the rhodiola rosea has the skin care effects of resisting light aging and fatigue, astragalus polysaccharide and astragaloside contained in the astragalus membranaceus can repair a barrier and enhance immunity, ophiopogonin and polysaccharide contained in the radix ophiopogonis have the effects of preserving moisture and resisting saccharification, and hesperidin and nobiletin contained in the pericarpium citri reticulatae have the effects of resisting oxidation and adjusting grease secretion. The obtained compound enzyme has the effects of resisting oxidation, resisting inflammation and preserving moisture, has activity, stability and transdermal efficiency, and can be used in skin care products such as anti-oxidation essence, repair masks, late-up cream and the like.

The present invention relates to a bone trauma repair bone paste and a preparation method thereof, belonging to the technical field of biomedical material manufacturing. The bone paste of the present invention is prepared by mixing nano calcium phosphate biphasic ceramics, biomimetic mineralized collagen fibers, biodegradable polymers, modified hyaluronic acid, β-cyclodextrin-coated recombinant human bone morphogenetic protein-7, angiogenesis promoters and immunomodulatory additives in a certain proportion; the nano calcium phosphate biphasic ceramics are hydroxyapatite and β-tricalcium phosphate biphasic ceramics; the biomimetic mineralized collagen fibers are prepared by mineralizing freeze-dried fibers of type I collagen admixed with hydroxypropyl chitosan; the modified hyaluronic acid is hyaluronic acid-thiol and hyaluronic acid-hydrazide; the angiogenesis promoter is a polylactic acid sustained-release microsphere loaded with tanshinone and hydroxy polyethylene glycol biotin; the immunomodulatory additive is disodium glycyrrhizinate, lentinan and astragalus polysaccharide. The bone paste can effectively improve the bone induction ability, promote angiogenesis and reduce the risk of immunogenicity.

The invention discloses a procyanidine compound hard capsule for a health food capable of maintaining beauty and keeping young. The procyanidine compound hard capsule is prepared from the following components: a procyanidine extract, chitosan, vitamins, collagen, hyaluronic acid, a lotus leaf extract, astragalus polysaccharide, casein phosphopeptides, D-mannitol, starch, magnesium stearate and gelatin. The invention relates to a production process and application of a procyanidine compound hard capsule for beauty and young keeping health food, and the production process comprises the following steps: step 1, adjusting a formula proportion by using a component proportion optimization model to obtain an optimal preparation effect; 2, solution preparation is conducted on all the components, and the prepared solutions are mixed together; step 3, drying the procyanidine compound solution by adopting low-temperature vacuum drying; and 4, metering the dried procyanidine compound powder by using a capsule filling machine, and sub-packaging the procyanidine compound powder into medicinal hard capsules. The beauty-maintaining, young-keeping and health-care effects are greatly improved.

The invention belongs to the technical field of animal immunopotentiators, and particularly relates to an oil-in-water type veterinary adjuvant and a preparation method thereof. The oil-in-water type veterinary adjuvant comprises white oil, span 80, Tween 80, PEG400, astragalus polysaccharide and a phosphate buffer solution, the astragalus polysaccharide cooperates with a surfactant to activate a TLR4 / NF-kappa B pathway, Th1 / Th2 / Th17 three-way immune response is achieved, the serum IgG and mucous membrane sIgA antibody level is remarkably improved, and the problems that a traditional O / W adjuvant is high in layering rate (smaller than 5%), and the stability of the traditional O / W adjuvant is poor are solved through compound optimization of the HLB value (HLB = 11.5-12.0) of the HLB value of span 80 and Tween 80. And storage at 4 DEG C for 90 days) and poor freeze-thaw stability are solved.

The invention discloses an agricultural synergist containing astragalus polysaccharide and application of the agricultural synergist, and belongs to the technical field of killing agents for biopesticides. The synergist comprises astragalus polysaccharide, a polysorbate / lecithin compound emulsifier, a carboxymethyl chitosan / siloxane penetrant and a modified accelerant synthesized by palladium catalysis. Polysaccharide is structurally modified through a blue light LED induced tetra (triphenylphosphine) palladium catalytic reaction, and the epidermal penetration efficiency of the biopesticide on tetranychus urticae is improved by 3.2 times in combination with the synergistic permeation effect of a siloxane derivative. The mite killing rate of the suspending agent prepared according to a preferable formula reaches 92.5% within 24 hours, and the lasting period is prolonged to 14 days. The system overcomes the defects of slow effectiveness and poor stability of the traditional biopesticide, by means of a targeted combination mechanism of natural components, the environment friendliness is kept while the original pesticide dosage is reduced by 50%, and the system is particularly suitable for the pest control requirement of organic agriculture.

The invention belongs to the technical field of biomedical materials, and particularly relates to a hydrogel dressing based on traditional Chinese medicine polysaccharide and a preparation method of the hydrogel dressing. The hydrogel dressing based on the traditional Chinese medicine polysaccharide is prepared from the following raw materials in parts by weight: 10 to 15 parts of gastrodia elata polysaccharide, 8 to 12 parts of rhizoma bletillae polysaccharide, 5 to 8 parts of astragalus polysaccharide, 1 to 3 parts of a nano-silver-baicalin compound, 2 to 3 parts of methacrylic anhydride, 0.02 to 0.05 part of VEGF, 0.4 to 1 part of PLGA, 0.1 to 0.3 part of Irgacure 2959, 20 to 30 parts of a 5% calcium chloride solution, 0.1 part of polyvinyl alcohol and 100 to 170 parts of PBS. A triple gel network structure is constructed through photocuring, ionic crosslinking and an emulsification-solvent evaporation method, so that the function integration of controlled release, antibiosis and vascular regeneration promotion is realized, and the clinical treatment requirements of complex wounds are effectively met.

The invention relates to an HA-APS (at) MSN-PDA-CS-mPEG nano particle and a preparation method of the nano particle. The invention relates to a preparation method of HA-APS (at) MSN-PDA-CS-mPEG nanoparticles. The preparation method comprises the following steps: (1) preparing mesoporous silica microspheres; (2) carrying out amination modification on the mesoporous silica microspheres, so as to obtain MSN-NH2; (3) coating the MSN-NH2 by using a PDA-CS-mPEG (Polyethylene Glycol) copolymer, so as to obtain MSN-PDA-CS-mPEG (Polyethylene Glycol); (4) the MSN-PDA-CS-mPEG is subjected to hyaluronic acid modification, and HA (at) MSN-PDA-CS-mPEG is obtained, and HA (at) MSN-PDA-CS-mPEG is obtained; and (5) loading the astragalus polysaccharide on the HA (at) MSN-PDA-CS-mPEG, so as to obtain the HA-APS (at) MSN-PDA-CS-mPEG nano particles. According to the HA-APS-coated MSN-PDA-CS-mPEG nano particle and the preparation method thereof, mesoporous silica is used as a nano carrier, copolymer micelles formed by PDA, CS and mPEG are coated on the surface of a silicon sphere, then HA modified by differentiated cluster protein C4DD is coated, finally astragalus polysaccharide is loaded, and a nano drug delivery system with a targeting effect is obtained by utilizing layer-by-layer structured modification. And the preparation method is high in universality.

The invention relates to the technical field of probiotic preparations, in particular to a process for preparing intestinal conditioning prebiotics from clostridium butyricum and radix astragali seu hedysari, which comprises the following steps: (1) multi-stage synergistic activation of clostridium butyricum; (2) carrying out composite enzymolysis on the astragalus extract; (3) symbiotic fermentation; (4) performing multi-layer embedding; the prepared prebiotics have a multi-dimensional intestine regulating mechanism, the bacteriostasis and mucous membrane repairing functions of the clostridium butyricum cooperate with the prebiotics effect and antioxidant activity of the astragalus polysaccharide to form a multi-protection system, and the intestinal flora imbalance is more effectively improved compared with single probiotics or prebiotics. The multi-layer embedding technology solves the problems of gastric acid inactivation and difficulty in intestinal colonization of probiotics, and spore induction of three-stage fermentation is matched, so that the viable count maintenance rate of the product is kept at a relatively high level after the product is stored for 6 months at normal temperature, and the shelf life is remarkably prolonged.

The invention discloses a formula composition which is prepared from the following components in parts by weight: 100 to 400 parts by weight of grape seed procyanidine, 100 to 300 parts by weight of pomegranate polyphenol, 10 to 100 parts by weight of ergothioneine, 50 to 200 parts by weight of catechin and 100 to 400 parts by weight of astragalus polysaccharide. According to the invention, cell-level anti-aging is taken as a core logic, five active ingredients of grape seed procyanidine, pomegranate polyphenol, ergothioneine, catechin and astragalus polysaccharide are scientifically compounded, a multi-dimensional anti-oxidation defense system is constructed, and multiple effects of scavenging free radicals, repairing cell damage, delaying an aging process and the like can be realized.

The invention relates to the technical field of veterinary biological products. The invention provides a poultry epidemic disease inactivated vaccine adjuvant as well as a preparation method and application thereof. The adjuvant is prepared from the following raw materials in dosage ratio: 70 to 80mL of white oil, 0.5 to 1.5 mL of Tween-80, 1 to 2g of chitosan, 0.5 to 1.5 g of astragalus polysaccharide and 0.5 to 1.5 g of vitamin E. According to the invention, multiple raw materials are utilized, and a triple synergistic mechanism of long-acting slow release, efficient activation and safety protection is formed through emulsion stabilization and targeted delivery at a physical level, multi-channel activation (including natural immunity, adaptive immunity, humoral immunity and mucosal immunity) at an immune level and synergistic oxidation resistance and inflammatory reaction regulation at a safety level; the immune response efficiency, the antibody duration and the safety of the vaccine can be remarkably improved, and the vaccine is particularly suitable for the special immune physiological characteristics of poultry.

The invention belongs to the technical field of agriculture, and particularly relates to an application of an astragalus extract containing astragalus polysaccharide in promoting plant growth. Purified astragalus polysaccharide is proved to have good growth regulation and stress resistance enhancement on plants, but the extraction and purification process of polysaccharide is high in cost, low in efficiency and difficult to meet the requirements of large-scale production, and the application in agriculture is limited. Provided in the present invention is the application of the astragalus extract containing astragalus polysaccharide, in particular the application of same as a plant growth promoter in promoting plant growth and enhancing plant stress resistance. The use concentration of astragalus polysaccharide is reduced, the application of astragalus polysaccharide in agriculture is promoted, and a new option for regulating plant growth in agriculture is provided.

The present invention relates to a composition of aliphatic emulsion preparation for malignant thoracicoabdominal hydrops and its preparation method. It is made up by adopting pure natural anticancer medicine elemene and at least one polysaccharide selected from ginseng polysaccharide, astragalus polysaccharide, acanthopanax polysaccharide and lyceum polysaccharide as raw material. Said invention can obviously raise solubility of medicine, increase stability of medicine, effectively remove and kill cancer cells and raise immunity of body.

The invention discloses a culture method for hypoxia culture of umbilical cord mesenchymal stem cells, which comprises the following steps: hypoxia pre-adaptive culture: inoculating umbilical cord mesenchymal stem cells in a culture medium containing bFGF (basic fibroblast growth factor), glutathione, astragalus polysaccharide and salidroside, and transferring the umbilical cord mesenchymal stem cells into a 5% O2 environment after the umbilical cord mesenchymal stem cells are preliminarily adhered to the wall in a normal oxygen environment; performing low-oxygen enrichment culture: replacing a second culture medium containing astragalus polysaccharide, salidroside, D-ribose, sodium pyruvate and D-glucose, and promoting efficient cell proliferation under 2% O2; performing function strengthening culture: adding tanshinone IIA and GSK-269962A, inducing cells to secrete VEGF under 1% O2, and maintaining high dryness. According to the method, through the synergistic effect of the three-stage gradient oxygen concentration and the specific serum-free culture medium, the cell proliferation multiple is remarkably increased, the total apoptosis rate is reduced, VEGF secretion is promoted, the multidirectional differentiation potential is reserved, the culture method is standardized in operation, the serum-free culture medium is definite in component, and a high-quality stem cell culture scheme is provided for the field of regenerative medicine.

The present invention discloses a nutritional paste and a preparation method thereof, and belongs to the technical field of animal feed preparation, the nutritional paste comprises a plurality of components, the eucommia ulmoides extract has good anti-inflammatory, analgesic and anti-stress effects, can relieve pain and inflammation after dogs and cats are sterilized, and can be used for preparing the nutritional paste for dogs and cats. And the astragalus extract (containing astragalus polysaccharide) has the effect of enhancing the immunity of the organism, so that the postoperative complications of dogs and cats after sterilization can be effectively prevented. The semen ziziphi spinosae, the gamma-aminobutyric acid and the tryptophan have the effects of calming and relieving restlessness, the lactoferrin can assist iron absorption and assist hematogenesis, meanwhile, the glutamine can promote wound healing, and the prebiotics can adjust intestinal flora imbalance caused by stress and use of antibiotics, so that postoperative gastrointestinal discomfort is avoided. The nutritional paste prepared by the invention can guarantee nutrient substances required by pet dogs and cats in the postoperative stage, promote wound healing of the pet dogs and cats after the operation, effectively relieve postoperative pain and restlessness, enhance the immunity of the organism and promote rapid recovery after the operation.

The invention relates to the technical field of pig feeds, and particularly discloses a special compound feed for weaning sows, the feed comprises a functional additive, the functional additive comprises the following raw materials in parts by weight: 1-3 parts of a plant extract compound, 2-5 parts of conjugated linoleic acid, 0.0003-0.0005 part of selenomethionine, 0.15-0.35 part of vitamin E, and 0.15-0.3 part of vitamin C; the plant extract compound is prepared from the following components: astragalus polysaccharide, icariin, epigallocatechin gallate and a carrier, and the mass ratio of the astragalus polysaccharide to the icariin to the epigallocatechin gallate is 1 to (1 to 4) to (0.2 to 0.8). The special compound feed for the weaned sows is used for feeding the weaned sows through a scientific and reasonable formula, can promote follicular development of the sows after weaning, improve the oestrus rate and reduce the proportion of non-oestrus sows, and can solve the problem of slow ovarian function recovery of the sows in summer high-temperature seasons.

The invention discloses an umbilical cord mesenchymal stem cell serum-free culture medium, which is composed of a basic culture medium and additive components, and the additive components comprise, by final concentration, 5-20 [mu] g / mL of astragalus polysaccharide, 0.5-2.0 [mu] g / mL of salidroside, 0.1-0.5 [mu] g / mL of tanshinone IIA, 0.1-0.3 mg / mL of sodium pyruvate, 5-15 mM of D-ribose, 3-6 mg / mL of recombinant human albumin, and 5-10 [mu] g / mL of recombinant transferrin; the invention also discloses a method for culturing umbilical cord mesenchymal stem cells under the hypoxia condition by using the serum-free culture medium. The method comprises the steps of cell inoculation, culture under the hypoxia condition, subculture, cell passage and the like. The astragalus polysaccharide, the salidroside, the tanshinone IIA, the sodium pyruvate, the recombinant human albumin and the recombinant transferrin are added into the serum-free culture medium, so that nutrition supply and function maintenance of cells under a low-oxygen condition are guaranteed, the proliferation rate and the differentiation capacity of the cultured cells are improved, the apoptosis rate of the cells is reduced, and the survival rate of the cells is increased. Wide application prospects are realized in the fields of cell culture, regenerative medicine and the like.