Feed containing astragalus polysaccharide and preparation method thereof

Abstract

The invention discloses a feed containing astragalus polysaccharide and a preparation method thereof, and belongs to the field of feeds. The astragalus polysaccharide is added into the basic feed, so that the adverse effect of heat stress on the female rabbits can be effectively relieved, the conception rate of the female rabbits, the number of live litters and the average weight of the live litters can be increased, and the breeding economic benefits are improved while the reproductive performance is optimized and the growth of the young rabbits is promoted; the prepared feed can effectively improve the serum hormone level of animals and reduce the inflammatory factor level, meanwhile, the diversity of animal intestinal microorganisms under heat stress is effectively improved, a scientific basis is provided for precise feeding management of domestic rabbits in summer, and meanwhile, an important reference is provided for application of astragalus polysaccharide in the field of livestock and poultry anti-stress.

Description

Technical Field

[0001] The present invention belongs to the field of feeds, and particularly relates to a feed containing astragalus polysaccharide and a preparation method thereof. Background Art

[0002] In a high-temperature and high-humidity environment, when the heat metabolism balance of livestock and poultry bodies is disrupted, heat stress reactions are likely to occur. Compared with other livestock, rabbits are more sensitive to heat stress due to their physiological structure characteristics. The high-temperature environment will not only significantly inhibit the feed intake of rabbits, but also have a significant negative impact on their overall production performance. Heat stress can cause an increase in the body's oxidative stress level and inflammation level, etc., resulting in a decrease in the estrus rate and conception rate of female rabbits, abnormal placental function, and restricted fetal growth and development.

[0003] Improving the reproductive performance of female rabbits is crucial for the rabbit breeding industry. The heat accumulation in female rabbits in a high-temperature environment has become a key factor restricting their reproductive performance, posing a significant threat to rabbit production in summer. When the environmental temperature exceeds the critical threshold, rabbits will initiate a heat stress compensation mechanism, leading to problems such as disordered secretion of reproductive hormones and embryo implantation disorders. Heat stress will cause a decrease in the reproductive ability of female rabbits, the survival rate of embryos, and the number of offspring per litter, thus affecting the lactation performance of female rabbits. Research shows that high temperature will cause an increase in the content of ROS in follicles, resulting in the apoptosis of granulosa cells (GCs), and then damaging the structure of oocytes, thereby weakening the quality of oocytes and their in vitro maturation and fertilization ability. At the same time, the increase in ROS will also affect the growth and maturation of oocytes, causing chromosomal DNA damage, resulting in a large number of ovarian cell apoptosis, thus inhibiting follicle development, and ultimately affecting the reproductive performance of rabbits, leading to a 30%-50% decrease in the conception rate and a reduction of 2-3 offspring per litter. In extreme cases, the mortality rate of heat stroke can reach more than 15%, seriously restricting the economic benefits of the industry. At the same time, in a high-temperature environment, the animal body will redistribute blood to enhance heat dissipation, resulting in insufficient blood supply to the internal organs (such as mammary glands, ovaries, and uterus) of pregnant female rabbits. Due to hypoxia, fetal development is hindered, leading to an increase in early embryo mortality. In addition, heat stress will also reduce the blood flow of the mammary gland, inhibit mammary gland development, reduce lactation performance, and further affect the milk yield by reducing dry matter intake and nutrient absorption.

[0004] In intensive farming in high-temperature seasons, breaking through the inhibition of heat stress on the reproductive performance of female rabbits has become the key point of technological breakthrough in the industry. Traditional cooling management methods have defects such as large equipment investment (the cooling cost per square meter increases by 40-60 yuan) and poor maintenance effect (the shed temperature only drops by 2-3°C), and it is difficult to meet the needs of large-scale production. Therefore, it is of urgent practical significance to seek a new type of low-cost and high-benefit nutritional intervention plan. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to provide a feed containing astragalus polysaccharide and a preparation method thereof, so as to solve the technical problem of reduced reproductive ability of animals under heat stress.

[0006] To achieve the above object, the technical solution adopted by the present invention is to provide a feed containing astragalus polysaccharide, which is a mixture of a basal diet and astragalus polysaccharide in a mass ratio of 1 kg: 200 - 1000 mg. The raw materials used in the basal diet include, by weight: 10 - 18 parts of corn, 2 - 6 parts of wheat middlings, 6 - 9 parts of wheat bran, 4 - 8 parts of Chinese wildrye, 10 - 20 parts of distillers' grains, 2 - 8 parts of peanut shells, 8 - 12 parts of soybean meal, 0.3 - 0.7 parts of soybean oil, 2 - 8 parts of white pole, 7 - 10 parts of malt root, 2 - 8 parts of palm kernel meal, 6 - 9 parts of rice husk, 5 - 10 parts of peanut vine powder, and 4 - 6 parts of premix.

[0007] On the basis of the above technical solution, the present invention can also be improved as follows:

[0008] Further, the feed is a mixture of a basal diet and astragalus polysaccharide in a mass ratio of 1 kg: 400 mg.

[0009] Further, the raw materials used in the basal diet include, by weight: 14 parts of corn, 4 parts of wheat middlings, 7.6 parts of wheat bran, 6 parts of Chinese wildrye, 15 parts of distillers' grains, 5 parts of peanut shells, 10 parts of soybean meal, 0.5 parts of soybean oil, 5 parts of white pole, 8.5 parts of malt root, 5 parts of palm kernel meal, 7.3 parts of rice husk, 7 parts of peanut vine powder, and 5.1 parts of premix.

[0010] Further, the raw materials used in the basal diet include, by weight: 10 parts of corn, 2 parts of wheat middlings, 6 parts of wheat bran, 8 parts of Chinese wildrye, 20 parts of distillers' grains, 8 parts of peanut shells, 8 parts of soybean meal, 0.3 parts of soybean oil, 8 parts of white pole, 10 parts of malt root, 2 parts of palm kernel meal, 6 parts of rice husk, 10 parts of peanut vine powder, and 6 parts of premix.

[0011] Further, the raw materials used in the basal diet include, by weight: 18 parts of corn, 6 parts of wheat middlings, 9 parts of wheat bran, 4 parts of Chinese wildrye, 10 parts of distillers' grains, 2 parts of peanut shells, 12 parts of soybean meal, 0.7 parts of soybean oil, 2 parts of white pole, 7 parts of malt root, 8 parts of palm kernel meal, 9 parts of rice husk, 5 parts of peanut vine powder, and 4 parts of premix.

[0012] Furthermore, per kilogram of the premix includes: 50 - 100 mg of Fe, 10 - 30 mg of Cu, 50 - 100 mg of Zn, 5 - 15 mg of Mn, 0.1 - 0.2 mg of Co, 0.1 - 0.3 mg of I, 0.2 - 0.3 mg of Se, 9000 - 11000 IU of VA, 800 - 1000 IU of VD, 30 - 70 mg of VE, 1 - 3 mg of VK, 1 - 3 mg of VB1, 4 - 8 mg of VB2, 30 - 70 mg of VB5, 1 - 3 mg of VB6, VB 12 0.01 - 0.03 mg, 30 - 70 mg of VB3, 30 - 60 mg of VB9, 900 - 1100 mg of VB4, and 0.1 - 0.3 mg of VB7.

[0013] Furthermore, per kilogram of the premix includes: 70 mg of Fe, 20 mg of Cu, 70 mg of Zn, 10 mg of Mn, 0.15 mg of Co, 0.2 mg of I, 0.25 mg of Se, 10000 IU of VA, 900 IU of VD, 50 mg of VE, 2 mg of VK, 2 mg of VB1, 6 mg of VB2, 50 mg of VB5, 2 mg of VB6, VB 12 0.02 mg, 50 mg of VB3, 44 mg of VB9, 1000 mg of VB4, and 0.2 mg of VB7.

[0014] The present invention also discloses a preparation method of a feed containing astragalus polysaccharide, comprising the following steps: pulverizing Chinese wildrye, white spruce, and rice husk, and then uniformly mixing them with the remaining raw materials in proportion to obtain a feed containing astragalus polysaccharide.

[0015] The beneficial effects of the present invention are as follows:

[0016] 1. By adding astragalus polysaccharide (APS) to the basal diet, the present invention can effectively alleviate the adverse effects of heat stress on female rabbits, improve the conception rate, litter size of live offspring, and average weight of live offspring of female rabbits, and while optimizing reproductive performance and promoting the growth of young rabbits, it realizes the improvement of breeding economic benefits, providing an effective nutritional regulation strategy for meat rabbit breeding in summer.

[0017] 2. By adding astragalus polysaccharide (APS) to the basal diet, it can significantly increase the levels of progesterone (P), estradiol (E2), and IL - 10 in the serum of breeding female rabbits, and at the same time significantly reduce the level of IL - 6, thereby effectively alleviating the inflammatory response caused by heat stress and reducing the adverse effects of heat stress on female rabbits.

[0018] 3. Adding Astragalus polysaccharide (APS) to the basal diet can effectively improve the intestinal microbial diversity of female rabbits under heat stress, and has a good regulatory effect on the intestinal microbial community and SCFAs. It can increase the abundances of beneficial bacteria (such as Firmicutes, Bacteroides, Akkermansia), and optimize the intestinal microecological balance. By promoting the synthesis of SCFAs (especially acetic acid, propionic acid, butyric acid), APS enhances the intestinal mucosal barrier function, exhibits anti-inflammatory activity, alleviates the dysbiosis, barrier damage and metabolic disorders induced by heat stress, reduces the health risks, and provides a theoretical basis for the application of APS in improving animal intestinal health and production performance in animal husbandry. Description of the Drawings

[0019] Figure 1 are the data of the temperature-humidity index in the rabbit house;

[0020] Figure 2 is the statistical chart of the heat stress state;

[0021] Figure 3 is the concentration of progesterone (P) in the serum of pregnant female rabbits on the 15th day under heat stress;

[0022] Figure 4 is the concentration of estradiol (E2) in the serum of pregnant female rabbits on the 15th day under heat stress

[0023] Figure 5 is the concentration of IL-10 in the serum of pregnant female rabbits on the 15th day under heat stress;

[0024] Figure 6 is the concentration of IL-6 in the serum of pregnant female rabbits on the 15th day under heat stress;

[0025] Figure 7 is the Venn diagram of the intestinal flora;

[0026] Figure 8 is the PCoA cluster analysis of the intestinal samples;

[0027] Figure 9 is the effect of different doses of APS on the relative abundances of rabbit intestinal flora at the phylum level;

[0028] Figure 10 is the effect of heat stress and Astragalus polysaccharide on the relative abundance value of Bacteroides at the genus level;

[0029] Figure 11 is the effect of heat stress and Astragalus polysaccharide on the relative abundance value of Ruminococcus at the genus level;

[0030] Figure 12 is the effect of heat stress and Astragalus polysaccharide on the relative abundance value of Alistipes at the genus level;

[0031] Figure 13 Effects of heat stress and Astragalus polysaccharide on the relative abundance values of the V9D2013 flora (V9D2013_group) at the genus level;

[0032] Figure 14 Effects of heat stress and Astragalus polysaccharide on the relative abundance values of Candidatus_Saccharimonas at the genus level;

[0033] Figure 15 Effects of heat stress and Astragalus polysaccharide on the relative abundance values of Akkermansia at the genus level;

[0034] Figure 16 Effects of heat stress and Astragalus polysaccharide on the relative abundance values of the Lachnospiraceae_NK4A136_group at the genus level;

[0035] Figure 17 Effects of heat stress and Astragalus polysaccharide on the relative abundance values of the Rikenellaceae_RC9_gut_group at the genus level;

[0036] Figure 18 For the LEfEse analysis chart;

[0037] Figure 19 For the species evolutionary branching diagram;

[0038] Figure 20 For the Spearman correlation coefficient;

[0039] Figure 21 Results of the correlation analysis between gut microbiota and short-chain fatty acids. Detailed implementation manners

[0040] The following describes the detailed implementation manners of the present invention to facilitate those skilled in the art of the present technology to understand the present invention. For those not specified in the examples, they are carried out according to conventional conditions or conditions recommended by the manufacturer. For reagents or instruments not specified for the manufacturer, they are all conventional products that can be obtained through commercial purchase. However, it should be clear that the present invention is not limited to the scope of the detailed implementation manners. For those ordinary skilled in the art of the present technology, as long as various changes are within the spirit and scope of the present invention defined and determined by the appended claims, these changes are obvious, and all inventions and creations using the concept of the present invention are within the scope of protection.

[0041] Example 1

[0042] A feed containing astragalus polysaccharide, which is prepared by mixing a basal diet and astragalus polysaccharide at a mass ratio of 1 kg: 400 mg. The raw materials used in the basal diet, by weight, include: 12 parts of corn, 5 parts of wheat middlings, 5 parts of corn bran, 8.5 parts of wheat bran, 1.5 parts of soybean oil, 14 parts of soybean meal, 6 parts of rapeseed meal, 9 parts of spent brewer's grains, 6.5 parts of corn germ meal, 1.5 parts of artemisia annua powder, 6 parts of rice hull powder, 5 parts of peanut hull powder, 7 parts of white rod powder, 8 parts of alkali grass, and 5 parts of premix.

[0043] Per kilogram of the premix includes: 70 mg of Fe, 20 mg of Cu, 70 mg of Zn, 10 mg of Mn, 0.15 mg of Co, 0.2 mg of I, 0.25 mg of Se, 10000 IU of VA, 900 IU of VD, 50 mg of VE, 2 mg of VK, 2 mg of VB1, 6 mg of VB2, 50 mg of VB5, 2 mg of VB6, VB 12 0.02 mg, 50 mg of VB3, 44 mg of VB9, 1000 mg of VB4, and 0.2 mg of VB7.

[0044] Example 2

[0045] A feed containing astragalus polysaccharide, which is prepared by mixing a basal diet and astragalus polysaccharide at a mass ratio of 1 kg: 200 mg. The raw materials used in the basal diet, by weight, include: 10 parts of corn, 3 parts of wheat middlings, 3 parts of corn bran, 5 parts of wheat bran, 0.5 part of soybean oil, 20 parts of soybean meal, 9 parts of rapeseed meal, 12 parts of spent brewer's grains, 8 parts of corn germ meal, 2.5 parts of artemisia annua powder, 3 parts of rice hull powder, 3 parts of peanut hull powder, 10 parts of white rod powder, 10 parts of alkali grass, and 7 parts of premix.

[0046] Per kilogram of the premix includes: 50 mg of Fe, 10 mg of Cu, 50 mg of Zn, 15 mg of Mn, 0.2 mg of Co, 0.3 mg of I, 0.3 mg of Se, 9000 IU of VA, 800 IU of VD, 70 mg of VE, 1 mg of VK, 1 mg of VB1, 4 mg of VB2, 30 mg of VB5, 3 mg of VB6, VB 12 0.03 mg, 70 mg of VB3, 60 mg of VB9, 1100 mg of VB4, and 0.3 mg of VB7.

[0047] Example 3

[0048] A feed containing astragalus polysaccharide, which is prepared by mixing a basal diet and astragalus polysaccharide at a mass ratio of 1 kg: 1000 mg. The raw materials used in the basal diet include, by weight: 15 parts of corn, 7 parts of wheat middlings, 7 parts of corn bran, 10 parts of wheat bran, 2.5 parts of soybean oil, 10 parts of soybean meal, 3 parts of rapeseed meal, 6 parts of spent brewer's grains, 5 parts of corn germ meal, 0.5 part of artemisia annua powder, 9 parts of rice hull powder, 7 parts of peanut shell powder, 5 parts of white rod powder, 5 parts of alkali grass and 3 parts of premix.

[0049] Each kilogram of the premix contains: 100 mg of Fe, 30 mg of Cu, 100 mg of Zn, 5 mg of Mn, 0.1 mg of Co, 0.1 mg of I, 0.2 mg of Se, 11000 IU of VA, 1000 IU of VD, 30 mg of VE, 3 mg of VK, 3 mg of VB1, 8 mg of VB2, 70 mg of VB5, 1 mg of VB6, VB 12 0.01 mg, 30 mg of VB3, 30 mg of VB9, 900 mg of VB4 and 0.1 mg of VB7.

[0050] Control group

[0051] The difference between this control group and Example 1 is that astragalus polysaccharide (APS) is omitted, and the other components are the same as those in Example 1, and a basal diet is prepared.

[0052] Experimental Example 1

[0053] 460 Yila multiparous does with similar body weight and parity are selected as the test objects and randomly divided into 5 groups (each group contains 92), which are respectively:

[0054] (1) Control group (CON group): fed the basal diet prepared in the control group;

[0055] (2) APS200 group: fed the basal diet prepared in the control group + 200 mg / kg APS;

[0056] (3) APS400 group: fed the basal diet prepared in the control group + 400 mg / kg APS;

[0057] (4) APS800 group: fed the basal diet prepared in the control group + 800 mg / kg APS;

[0058] (5) APS1000 group: fed the basal diet prepared in the control group + 1000 mg / kg APS.

[0059] Starting from the day when light was increased in the rabbit house (i.e., 6 days before breeding), the corresponding feed was fed to the female rabbits in each experimental group until the 35th day after parturition, with a total cycle of 71 days. The nutritional levels of the basal diet used are shown in Table 1.

[0060] Table 1 Nutritional levels of the basal diet for female rabbits (air-dried basis)

[0061]

[0062]

[0063] In this experiment, a 42-day breeding model was adopted, that is, the female rabbits were bred 12 days after parturition. On the day of breeding, artificial insemination technology was used to perform the breeding operation on the female rabbits; on the 15th day after breeding, the pregnancy status of the female rabbits was confirmed by palpation examination, and the conception rate was calculated; when the female rabbits entered the parturition stage, multiple indicators including reproductive performance were recorded in detail, and the growth performance indicators of the offspring rabbits were statistically analyzed subsequently.

[0064] 1. Evaluation of heat stress status

[0065] (1) Measurement of temperature-humidity index

[0066] A temperature-humidity recorder was placed in the rabbit house to automatically collect the temperature and humidity data of the rabbit house. From the day of breeding until the end of parturition of the female rabbits, the data was recorded every 30 minutes, and the temperature and humidity values at 14:00, 16:00, and 18:00 every day were selected. The temperature-humidity index (THI) was calculated according to the values of dry-bulb temperature and relative humidity. The calculation formula is:

[0067] THI = db - [(0.31 - 0.31RH)(db - 14.4)];

[0068] Among them, THI is the temperature-humidity index, %; db is the dry-bulb temperature, °C; RH is the relative humidity, %.

[0069] (2) Test results

[0070] The data of the temperature-humidity index (THI) are as Figure 1 shown. The threshold THI of heat stress in rabbits is 27.8. According to the THI evaluation value, the degree of heat stress faced by rabbits is divided into the following categories:

[0071] When THI < 27.8, the rabbits are not in a heat stress state;

[0072] When 27.8 ≤ THI < 28.9, it indicates that the rabbits are in a mild heat stress state;

[0073] When 28.9 ≤ THI < 30.0, the rabbits are in a moderate heat stress state;

[0074] When THI ≥ 30.0, the rabbits are in a state of severe heat stress.

[0075] It is assumed that if more than 50% of the time reaches the heat stress state during the entire test period, the test environment is determined to meet the test conditions for heat stress.

[0076] From Figure 2 It can be seen that during the entire test process, the time proportion of the female rabbits in a state of mild heat stress is 3.33%, the time proportion in a state of moderate heat stress is 26.67%, and the time proportion in a state of severe heat stress reaches 50%. This shows that the time proportion of the female rabbits suffering from different degrees of heat stress is as high as 80%, far exceeding 50%. Therefore, it can be clearly determined that the test conditions of this test have reached the heat stress standard, laying a foundation for subsequent research on the effects of heat stress on female rabbits.

[0077] 2. Effects of Astragalus polysaccharide on the reproductive performance of female rabbits

[0078] (1) Determination of the reproductive performance of female rabbits

[0079] The female rabbits were induced to estrus on the first day of the pre-feeding period by light. After the female rabbits were in estrus, artificial insemination was used for breeding on the first day of the test period, and the number of female rabbits participating in breeding was recorded. On the 15th day after breeding, the abdomen was palpated to determine whether the female rabbits were pregnant, and the conception rate of each group was recorded. After the female rabbits gave birth, the litter-related situations of each female rabbit were recorded, including multiple reproductive performance indicators such as the littering rate, litter size, number of live-born offspring per litter, total weight of offspring per litter, total weight of live-born offspring per litter, and number of dead fetuses. Among them, the conception rate refers to the proportion of female rabbits that are actually successfully pregnant after breeding, and the littering rate is the proportion of female rabbits that actually give birth after pregnancy. The specific calculation formulas are as follows:

[0080] A = (a / m) × 100%;

[0081] B = (b / m) × 100%;

[0082] Among them, A is the conception rate, %; a is the number of pregnant female rabbits, only; m is the number of female rabbits participating in breeding, only; B is the littering rate, %; b is the number of female rabbits giving birth, only.

[0083] (2) Test results

[0084] The data of the reproductive performance of female rabbits are shown in Table 2.

[0085] Table 2 Effects of adding Astragalus polysaccharide during the whole pregnancy period on the reproductive performance of female rabbits under heat stress

[0086]

[0087] Note: Different lowercase letters after the same-row values in the table indicate significant differences, P<0.05; control group (basic diet), APS200 group (basic diet + 200 mg / kg APS), APS400 group (basic diet + 400 mg / kg APS), APS800 group (basic diet + 800 mg / kg APS), APS1000 group (basic diet + 1000 mg / kg APS).

[0088] As can be seen from the data in Table 2, compared with the control group, the conception rates of female rabbits with 200, 400, 800, and 1000 mg / kg APS added to the feed were significantly increased (P<0.05) by 15.53%, 16.03%, 13.97%, and 20.91% respectively. In addition, the litter sizes of the APS400 and APS800 groups were significantly increased (P<0.05), by 21.64% and 18.59% respectively, while there were no significant differences between the other groups and the control group. The number of live born per litter in each experimental group was significantly higher than that in the control group (P<0.05), increasing by 0.31, 0.85, 0.62, and 0.42 respectively; the number of live born per litter also increased, by 0.33, 0.83, 0.63, and 0.40 respectively; there were no significant differences in the number of stillborn per litter among the groups. At the same time, the total litter weight and the live litter weight in all experimental groups were significantly increased compared with the control group (P<0.05). In the APS400 and APS800 groups, the average weight of the newly born live rabbits was significantly increased (P<0.05).

[0089] Since the number of live born per litter and the average weight of live rabbits are important indicators to measure the production efficiency of rabbit farms, from the above results, it can be seen that adding 400 mg / kg APS to the diet can significantly improve the reproductive performance indicators of female rabbits under heat stress conditions (P<0.05), indicating that 400 mg / kg is the optimal amount to relieve heat stress.

[0090] 3. Effects of Astragalus polysaccharide on the growth of young rabbits

[0091] (1) Determination of the growth performance of young rabbits

[0092] The young rabbits were evenly distributed into each litter after birth. At 5 days after birth, the hair began to grow; the eyes of the young rabbits opened after 12 days; and they were weaned at 35 days. Record the number of young rabbits per litter and the total weight of the young rabbits per litter at 7 days, 14 days, 21 days, and 35 days, and calculate the average weight accordingly.

[0093] (2) Experimental results

[0094] The data on the growth performance of young rabbits are shown in Table 3.

[0095] Table 3 Effects of adding Astragalus polysaccharide during the whole pregnancy on the growth of young rabbits under heat stress

[0096]

[0097]

[0098] Note: Different lowercase letters after the same-row numerical values in the table indicate significant differences, P < 0.05.

[0099] As can be seen from Table 3, on the 7th day of the young rabbits, compared with the control group, the litter size and the litter weight of the young rabbits in each experimental group were significantly increased (P < 0.05), and the average weight of the young rabbits in the APS400 group and the APS800 group also increased significantly (P < 0.05). On the 14th day, the litter weight and the average weight of the young rabbits in the 4 experimental groups were significantly higher than those in the control group (P < 0.05). By the 21st day, the litter weight of the young rabbits in each experimental group increased significantly (P < 0.05). At the same time, the average weight of the young rabbits in the APS400, APS800, and APS1000 groups also increased significantly (P < 0.05). Until the weaning at the 35th day, compared with the control group, the litter weight and the average weight of the young rabbits in each experimental group were still significantly increased (P < 0.05), indicating that APS at a dose of 400 mg / kg had a significant promoting effect on the growth of young rabbits under heat stress conditions.

[0100] During the period from the 1st day of pregnancy to the weaning of the young rabbits, the addition of APS significantly improved the growth performance of the young rabbits. Especially at the 7th day, 14th day, 21st day, and the 35th day of weaning, the litter weight and the average weight of the young rabbits in the APS400 group were significantly increased. In addition, the feed intakes of the female rabbits in the 400 and 800 mg / kg APS groups were significantly increased during the early and middle pregnancy periods and lactation compared with the control group and other experimental groups. Combining the reproductive performance and the growth data of the young rabbits, the APS400 group performed the best. Therefore, adding 400 mg / kg APS to the diet can significantly improve its reproductive performance and the growth performance of the offspring.

[0101] 4. Effects of Astragalus polysaccharide on the feed intake of female rabbits

[0102] (1) Recording of the feed intake of female rabbits

[0103] The feed intakes of female rabbits were recorded separately during the early pregnancy period (i.e., from the 0th day to the 10th day of pregnancy), the middle pregnancy period (from the 11th day to the 20th day of pregnancy), the late pregnancy period (from the 21st day to the 30th day of pregnancy), and lactation. The daily feed intake was the sum of the weight of the experimental feed fed in the afternoon every day and the weight of the supplementary feed in the next morning.

[0104] (2) Test results

[0105] The feed intake data of female rabbits are shown in Table 4.

[0106] Table 4 Effects of adding Astragalus polysaccharide on the feed intake of female rabbits under heat stress

[0107]

[0108] Note: Different lowercase letters after the same-row numerical values in the table indicate significant differences, P < 0.05.

[0109] As can be seen from Table 4, after adding Astragalus polysaccharide, the feed intake of female rabbits in the early and mid-pregnancy periods and the average daily feed intake during pregnancy were significantly increased (P < 0.05); in the late pregnancy, the feed intake of the APS400 and APS800 groups was significantly increased (P < 0.05); during lactation, the feed intake of the APS400, APS800, and APS1000 groups was significantly higher than that of the control group (P < 0.05). The test results show that APS has a significant promoting effect on improving the feed intake of female rabbits.

[0110] Experimental Example 2

[0111] At 8:00 am on the 15th day of pregnancy of female rabbits, 6 female rabbits were randomly selected from each group to collect ear vein blood, and then centrifuged at 2500 rpm for 10 min to collect serum for detecting the indexes of P, E2, IL-6, and IL-10.

[0112] The information of the manufacturers of the reagents used in the detection is shown in Table 5.

[0113] Table 5 Information of the manufacturers of reagents

[0114] Serial Number Name Manufacturer 1 Progesterone (P) Detection Kit Northern Biotechnology Research Institute 2 Estradiol (E2) Detection Kit Northern Biotechnology Research Institute 3 Interleukin-6 (IL-6) Detection Kit Thermo Fisher Scientific 4 Interleukin-10 (IL-10) Detection Kit Thermo Fisher Scientific

[0115] 1. Effects of APS on serum sex hormones of female rabbits on the 15th day of pregnancy

[0116] During pregnancy, the secretion level of steroid hormones usually gradually increases to ensure the smooth progress of pregnancy and the coordination of various physiological functions. P plays a crucial role in the process of pregnancy, while E2 plays a key role in triggering the labor mechanism. As Figure 3 can be seen, in the serum of female rabbits on the 15th day of heat-stressed pregnancy, compared with the control group, adding 400 mg / kg and 800 mg / kg doses of APS in the diet significantly increased the concentration of progesterone (P) (P < 0.05); as Figure 4 shown, the concentration of estradiol (E2) in the 200 mg / kg APS group was also significantly increased compared with the control group (P < 0.05). The above results show that the 400 mg / kg dose of APS has a significant effect on increasing the levels of progesterone and estradiol in the serum of female rabbits in the mid-pregnancy, indicating that APS can effectively regulate the reproductive endocrine system of female rabbits in a heat-stressed environment.

[0117] 2. Effects of APS on serum inflammatory indexes of heat-stressed female rabbits on the 15th day of pregnancy

[0118] AsFigure 5 and Figure 6 As shown in Figure 6 , compared with the control group, the IL-10 levels in the serum of female rabbits in each experimental group were significantly increased (P<0.05). At the same time, the serum IL-6 concentrations in the APS400 and APS800 groups were significantly decreased (P<0.05). In summary, adding APS at a dose of 400 mg / kg has a positive effect on effectively reducing the inflammatory response of female rabbits. APS effectively alleviated the inflammatory response caused by heat stress by decreasing the IL-6 level and increasing the IL-10 content.

[0119] In summary, under heat stress conditions, 400 mg / kg of astragalus polysaccharide can significantly improve the reproductive ability of female rabbits, promote the growth and development of offspring rabbits, and effectively reduce the adverse effects of heat stress on female rabbits.

[0120] Experimental Example 3

[0121] To explore the effects of astragalus polysaccharide on the intestinal microbiota and volatile fatty acids of female rabbits under heat stress conditions, 460 Yila female rabbits with similar body weights and parities were selected in this experimental example and randomly divided into five groups, namely the control CON group (basic diet), the APS200 group (200 mg / kg APS added to the basic diet), the APS400 group (400 mg / kg APS added to the basic diet), the APS800 group (800 mg / kg APS added to the basic diet), and the APS1000 group (1000 mg / kg APS added to the basic diet). The effects of astragalus polysaccharide on the intestinal microbial community of female rabbits under heat stress were studied from multiple aspects such as the number of OTUs, diversity index, and flora composition.

[0122] Figure 7 As shown in the Venn diagram of the intestinal flora, after analyzing the fecal samples, a total of 26,712 operational taxonomic units (OTUs) were identified. Among them, the CON group contained 2,836 OTUs, the APS200 group contained 3,034 OTUs, the APS400 group contained 3,850 OTUs, the APS800 group contained 3,408 OTUs, and the APS1000 group contained 3,501 OTUs. There were 844 OTUs common to these five groups, accounting for 18.86% of the total number of OTUs. From the total number of OTUs, the order was APS400 group > APS1000 group > APS800 group > APS200 group > CON group. The results showed that compared with the CON group, the types of intestinal microorganisms in each experimental group increased, thereby enhancing the diversity level of their microbial flora.

[0123] 2. Alpha Diversity Analysis

[0124] The Chao1 index is a key indicator for measuring the abundance of the microbial community. An increase in its value means an increase in the abundance of the microbial community; while the Simpson index and Shannon index represent the microbial diversity. The Simpson index reflects diversity by showing the proportion of dominant species and the balance of species distribution. An increase in its value means a decrease in diversity; on the contrary, the Shannon index is directly proportional to the microbial diversity, and the higher the value, the richer the diversity.

[0125] The data on the effect of APS on the α-diversity of intestinal microbiota in heat-stressed female rabbits are shown in Table 6. As can be seen from Table 6, compared with the CON group, the Chao1 index and Shannon index in the APS400, APS800, and APS1000 groups showed an upward trend. The Simpson index in the APS200 group increased slightly compared with the CON group, and the Simpson index in other experimental groups showed a downward trend compared with the control group. This indicates that APS may help to improve the diversity and richness of the microbial community to some extent.

[0126] Table 6 Alpha diversity index

[0127]

[0128] 3. Beta diversity analysis

[0129] β-diversity analysis aims to evaluate the differences in the intestinal microbiota structure between different samples or groups. By using the weighted Unifrac method, the microbial distances between groups can be quantified and compared, so as to reveal the similarities and differences in the composition of the microbial communities of each group. The increase in the discrete distance between groups is positively correlated with the enhancement of the differences between groups, that is, the closer the sample points are clustered, the smaller the differences between groups.

[0130] As Figure 8 shown, in the results of the principal coordinate analysis (PCoA) for intestinal samples, the contribution of the first principal component (PCoA1) to the differentiation of rumen microorganisms reached 12.1%, while the second principal component (PCoA2) contributed 7.1% of the differentiation. Through PCoA analysis, it was observed that there was a certain discreteness in the sample distribution between the heat stress group and the control group, which reflected significant differences in the microbiota structure among individuals within the group. At the same time, the fecal samples of the CON group, APS200, APS400, and APS800 groups also showed obvious dispersion characteristics, indicating that different doses of APS had different effects on the population structure of rabbit intestinal microbiota.

[0131] 4. Effects of Astragalus polysaccharide on the composition of rabbit intestinal microbiota at the phylum level

[0132] In the intestinal microecosystem, Firmicutes and Bacteroidota, as the main core bacterial groups, play a crucial role in regulating the host's energy balance, nutrient absorption, and intestinal physiological functions. Firmicutes have rich gene clusters related to sugar metabolism, which can efficiently convert the energy in the diet and promote fat accumulation; while Bacteroidota produces SCFAs by degrading substances such as cellulose and oligosaccharides, which can not only enhance the peristaltic function of the intestine but also strengthen the intestinal epithelial barrier and improve the mucosal immune response ability.

[0133] By deeply analyzing the composition and relative abundance of intestinal bacteria at the phylum level, the results are shown in Table 7 and Figure 9 as follows. It can be seen that a total of 20 phyla of bacteria were detected in the rabbit intestine, and these phyla were distributed in all five groups. According to the abundance ranking from high to low, the main phyla were: Firmicutes, Bacteroidota, Cyanobacteria, Patescibacteria, Proteobacteria, Verrucomicrobiota, Desulfobacterota, Campilobacterota, Fibrobacterota, Actinobacteriota, and Euryarchaeota, etc. At the phylum classification level, the top six species in the total abundance of each group of bacteria remained the same, and both Firmicutes and Bacteroidota occupied a core position. In the CON group, the proportions of Firmicutes and Bacteroidota were 61.61% and 29.84% respectively; in the APS 200 group, they were 52.00% and 36.86%; in the APS400 group, they were 52.98% and 31.21%; in the APS800 group, they were 54.16% and 30.57%; in the APS1000 group, they were 53.35% and 26.66%. According to the data analysis results in Table 7, the abundance of Firmicutes in each experimental group was significantly increased compared with the control group (P<0.05). Among Bacteroidota, the abundance in the APS200 group was significantly higher than that in the control group (P<0.05), while the other experimental groups showed a decreasing trend. For Cyanobacteria, the abundance in the APS800 and 1000 groups was significantly higher than that in the control group (P<0.05), and the other experimental groups showed an increasing trend. The abundance of Patescibacteria increased in each experimental group compared with the control group. The abundance of Proteobacteria in the APS400 group was significantly higher than that in the control group (P<0.05), showing an upward trend in the APS200 and APS1000 groups, while showing a downward trend in the APS800 group. The abundance of Verrucomicrobiota increased in the APS200, APS400, and APS800 groups compared with the control group.

[0134] Table 7 Effects of APS at different doses on the relative abundances of rabbit intestinal flora at the phylum level

[0135]

[0136]

[0137] 5. Effects of Astragalus polysaccharide on the composition of rabbit intestinal flora at the genus level

[0138] By analyzing the composition and relative abundances of intestinal bacteria at the genus level, the results are shown in Table 8 and Figure 10 as follows. When classifying the intestinal flora to the genus level, a total of 308 genera were identified. Among them, 13 genera had a relative proportion exceeding 1% of the total sequence number, specifically: Bacteroides, Ruminococcus, Alistipes, V9D2013_group, Candidatus_Saccharimonas, Akkermansia, Lachnospiraceae_NK4A136_group, Rikenellaceae_RC9_gut_group, Monoglobus, Phascolarctobacterium, UCG-005, and Colidextribacter.

[0139] Figure 10 - Figure 17The results showed significant differences in the intestinal flora between the control group and the experimental group supplemented with astragalus polysaccharides. In each group, the nine dominant genera with the highest relative abundance and their proportions were: Bacteroides (22.94%), Ruminococcus (28.57%), Alternaria (8.03%), V9D2013 flora (7.70%), Saccharomonas (3.21%), Akkermansia (1.92%), Lachnospiraceae NK4A136 flora (2.67%), and Rikenbacteriaceae RC9 intestinal flora (6.92%). In the APS200 group, the proportions were Bacteroides (44.78%), Ruminococcus (15.30%), Alternaria (8.21%), V9D2013 flora (4.36%), Saccharomonas (4.28%), Akkermansia (2.99%), Lachnospiraceae NK4A136 flora (2.87%), and Rikenbacteriaceae RC9 intestinal flora (0.25%). In the APS400 group, the proportions were Bacteroides (20.46%), Ruminococcus (17.42%), Alternaria (10.74%), V9D2013 flora (4.89%), Saccharomonas (6.42%), Akkermansia (2.19%), Lachnospiraceae NK4A136 flora (1.57%), and Rikenbacteriaceae RC9 intestinal flora (1.49%). In the APS800 group, the proportions were Bacteroides (21.67%), Ruminococcus (26.50%), Alternaria (7.98%), V9D2013 flora (7.70%), Saccharomonas (7.00%), Akkermansia (4.26%), Lachnospiraceae NK4A136 flora (3.16%) and Rikenbacteriaceae RC9 intestinal flora (0.57%). In the AP S1000 group, the proportions were Bacteroides (18.93%), Ruminococcus (25.25%), Alternaria (11.27%), V9D2013 flora (8.43%), Saccharomonas (9.40%), Akkermansia (2.13%), Lachnospiraceae NK4A136 flora (1.83%) and Rikenbacteriaceae RC9 intestinal flora (0.10%), which further illustrates that different doses of astragalus polysaccharides have different effects on the structure of intestinal flora.

[0140] like Figure 10 - Figure 17As shown, compared with the control group, Bacteroides in the APS200 group increased significantly (P<0.05), and there was a downward trend in other experimental groups. The relative abundance of Ruminococcus in the APS200 group decreased significantly (P<0.05), and other experimental groups also showed a certain downward trend. Alistipes showed an upward trend in each experimental group. The content of V9D2013_group in the APS800 and APS1000 groups had an upward trend. The abundances of Candidatus_Saccharimonas and Akkermansia increased in the experimental groups. The Lachnospiraceae_NK4A136_group in the APS400 group had a downward trend. The abundances of Rikenellaceae_RC9_gut_group in the experimental groups decreased significantly (P<0.05).

[0141] Table 8 Effects of different doses of APS on the relative abundances of rabbit intestinal flora at the genus level

[0142]

[0143] The distribution of dominant flora from phylum to genus in each group is as Figure 18 and Figure 19 shown. In the CON group, the dominant flora included Bacteroidetes - Bacteroidia - Bacteroidales - Maribacteraceae ( p_Bacteroidota.c_Bacteroidia.o_ Bacteroidales.f_Marinifilaceae ), Bacteroidetes - Bacteroidia - Bacteroidales - Maribacteraceae - Odoribacter ( p_Bacteroidota.c_Bacteroidia.o_Bacteroidales.f_Marinifilaceae.g_ Odoribacter ), and Firmicutes - unclassified class - DTU014 order ( p_Firmicutes.c_Incertae_Sedis.o_ DTU014 ). In the APS200 group, the dominant flora included Bacteroidetes - Bacteroidia - Bacteroidales - Rikenellaceae - RC9 gut group - unclassified RC9 gut species ( p_Bacteroidota.c_Bacteroidia.o_Bacteroidales.f_ Rikenellaceae.g_Rikenellaceae_RC9_gut_group.s_unclassified_g_Rikenellaceae_ RC9_gut_group ), Firmicutes - Clostridia - Oscillospirales - UCG_011 family ( p_Firmicutes.c_ Clostridia.o_Oscillospirales.f_UCG_0 ), and Bacteroidetes - Bacteroidia - Bacteroidales - Bacteroidaceae ( p_ Bacteroidota.c_Bacteroidia.o_Bacteroidales.f_Bacteroidaceae ). In the APS400 group, the dominant flora included Cyanobacteria - Vampirovibrionia - Gastranaerophilales - unclassified Gastranaerophilaceae ( p_Cyanobacteria.c_ Vampirivibrionia.o_Gastranaerophilales.f_unclassified_o_Gastranaerophilales ), Firmicutes - Clostridia - Lachnospirales - Lachnospiraceae - Acetanaerobacterium - Firmicutes ( p_Firmicutes.c_ Clostridia.o_Lachnospirales.f_Lachnospiraceae.g_Acetitomaculum.s_Firmicutes_ bacterium_CAG_194_44_15), and *Eubacterium sp.* ( p_Firmicutes.c_Clostridia.o_Peptostreptococcales_ Tissierellales.f_Anaerovoracaceae.g__Eubacterium__nodatum_group.s_ unclassified_g__Eubacterium__nodatum_group ). In the APS800 group, the dominant flora included *Alistipes obesi* ( p_Bacteroidota.c_ Bacteroidia.o_Bacteroidales.f_Rikenellaceae.g_Alistipes.s_Alistipes_obesi ), unclassified bacteria in the phylum Proteobacteria ( p_Proteobacteria.c_unclassified_p_ Proteobacteria.o_unclassified_p_Proteobacteria.f_unclassified_p_ Proteobacteria ), and *Bacteroides* ( p_ Bacteroidota.c_Bacteroidia.o_Bacteroidales.f_Bacteroidaceae.g_Bacteroides ). In the APS1000 group, the dominant flora included *Acidaminococcus sp.* ( p_Firmicutes.c_Negativicutes.o_Acidaminococcales.f_ Acidaminococcaceae.g_unclassified_f_Acidaminococcaceae ), Cyanobacteria ( p_ Cyanobacteria ), and unclassified Bacteroidales ( p_Bacteroidota.c_ Bacteroidia.o_unclassified_c_Bacteroidia ).

[0144] The above results indicate that APS has a positive impact on intestinal health by regulating the structure of the intestinal flora, especially by increasing the abundance of beneficial bacteria and promoting the production of SCFAs. These findings provide important experimental evidence for a deeper understanding of the regulatory effect of APS on the intestinal microecology.

[0145] 6. Effect of Astragalus polysaccharide on volatile fatty acids in rabbits

[0146] Short-chain fatty acids (SCFAs) are small molecular organic carboxylic acids containing 1 to 6 carbon atoms, which are mainly produced by anaerobic fermentation of resistant starch, inulin, cellulose, pectin, etc. in the intestine. SCFAs are classified into formic acid, acetic acid, propionic acid, butyric acid, valeric acid and hexanoic acid according to the number of carbon atoms they contain. Acetic acid, propionic acid and butyric acid are the main forms of SCFAs, while the concentrations of formic acid, valeric acid and hexanoic acid are at relatively low levels.

[0147] Table 9 Effect of Astragalus polysaccharide on volatile fatty acids in the intestine of female rabbits

[0148]

[0149]

[0150] As shown in Table 9, compared with the control group, the contents of acetic acid, butyric acid, caproic acid, propionic acid and valeric acid showed an increasing trend in the APS200 group; the content of caproic acid showed an increasing trend in the APS400 group; the contents of heptanoic acid, capric acid and octanoic acid increased significantly in the APS800 group (P<0.05). The contents of caproic acid, capric acid, heptanoic acid and octanoic acid showed an increasing trend in the APS1000 group.

[0151] The contents of SCFAs such as acetic acid, butyric acid and propionic acid in the APS200 treatment group increased, indicating that APS promoted the energy supply of intestinal epithelial cells, enhanced the intestinal barrier, effectively resisted pathogenic bacteria and inhibited inflammation. In the high-concentration treatment groups of APS800 and APS1000, medium- and long-chain fatty acids such as caproic acid, capric acid and heptanoic acid increased significantly, which could supply energy for intestinal epithelial cells, be beneficial to enhancing the intestinal barrier, and thus improve intestinal health. The increase in capric acid and heptanoic acid might also inhibit the growth of pathogenic bacteria and maintain the balance of the intestinal flora. The increase in medium- and long-chain fatty acids was also related to the optimization of energy metabolism and the reduction of the risk of metabolic diseases. Isobutyric acid and isovaleric acid showed an upward trend in the APS200 and APS1000 treatment groups, which might reflect the enhanced protein metabolism activity of the intestinal flora, contribute to improving the energy metabolism state, regulating the immune system, reducing intestinal inflammation, and improving inflammatory bowel disease (IBD), etc. Octanoic acid increased in the APS800 and APS1000 treatment groups, which was due to the high-dose APS changing the metabolic pathway of the intestinal flora, resulting in the accumulation of octanoic acid precursors or the enhanced activity of producing bacteria. The increase in octanoic acid might enhance the antibacterial effect, provide energy, and further improve intestinal health and host metabolism. Valeric acid showed an upward trend in the APS200 and APS800 groups, having a positive impact on intestinal health.

[0152] 7. Correlation analysis of rabbit intestinal microbiota and short-chain fatty acids

[0153] It can be seen from Figure 20 and Figure 21 that UCG-005, Oscillibacter, V9D2013_group, Colidextribacter, and Monoglobus were significantly positively correlated with SCFAs. Among them, UCG-005 was significantly positively correlated with the contents of isovaleric acid and octanoic acid (P<0.05), Oscillibacter was significantly positively correlated with acetic acid and caproic acid (P<0.05), and V9D2013_group was significantly positively correlated with butyric acid, isobutyric acid (P<0.05), propionic acid, valeric acid, and isovaleric acid (P<0.01). Colidextribacter was significantly positively correlated with multiple SCFAs, including acetic acid, valeric acid, isovaleric acid and caproic acid (P<0.05), as well as butyric acid and propionic acid (P<0.01); Monoglobus was extremely significantly positively correlated with the contents of acetic acid, butyric acid and propionic acid (P<0.01).

[0154] In summary, under heat stress, APS has a good regulatory effect on the intestinal microbial community and SCFAs, can increase the abundances of beneficial bacteria (such as Firmicutes, Bacteroides, Akkermansia), and optimize the intestinal microecological balance. By promoting the synthesis of SCFAs (especially acetic acid, propionic acid, butyric acid), APS enhances the intestinal mucosal barrier function, exhibits anti-inflammatory activity, alleviates the microbiota imbalance, barrier damage, and metabolic disorders induced by heat stress, and reduces the health risks. Therefore, as a natural prebiotic, APS has important application potential in improving animal intestinal health and enhancing production performance.

Claims

1. A feed containing astragalus polysaccharide, characterized in that, The feed is prepared by mixing a basic diet and astragalus polysaccharide in a mass ratio of 1 kg: 200 - 1000 mg. The raw materials used in the basic diet include, by weight parts: 10 - 15 parts of corn, 3 - 7 parts of wheat middlings, 3 - 7 parts of corn bran, 5 - 10 parts of wheat bran, 0.5 - 2.5 parts of soybean oil, 10 - 20 parts of soybean meal, 3 - 9 parts of rapeseed meal, 6 - 12 parts of distillers grains, 5 - 8 parts of corn germ meal, 0.5 - 2.5 parts of artemisia annua powder, 3 - 9 parts of rice hull powder, 3 - 7 parts of peanut shell powder, 5 - 10 parts of white rod powder, 5 - 10 parts of alkali grass, and 3 - 7 parts of premix.

2. The feed containing astragalus polysaccharide according to claim 1, characterized in that, The feed is prepared by mixing a basic diet and astragalus polysaccharide in a mass ratio of 1 kg: 400 mg.

3. The feed containing astragalus polysaccharide according to claim 1, wherein The raw materials used in the basic diet include, by weight parts: 12 parts of corn, 5 parts of wheat middlings, 5 parts of corn bran, 8.5 parts of wheat bran, 1.5 parts of soybean oil, 14 parts of soybean meal, 6 parts of rapeseed meal, 9 parts of distillers grains, 6.5 parts of corn germ meal, 1.5 parts of artemisia annua powder, 6 parts of rice hull powder, 5 parts of peanut shell powder, 7 parts of white rod powder, 8 parts of alkali grass, and 5 parts of premix.

4. The feed containing astragalus polysaccharide according to claim 1, characterized in that, The raw materials used in the basic diet include, by weight parts: 10 parts of corn, 3 parts of wheat middlings, 3 parts of corn bran, 5 parts of wheat bran, 0.5 parts of soybean oil, 20 parts of soybean meal, 9 parts of rapeseed meal, 12 parts of distillers grains, 8 parts of corn germ meal, 2.5 parts of artemisia annua powder, 3 parts of rice hull powder, 3 parts of peanut shell powder, 10 parts of white rod powder, 10 parts of alkali grass, and 7 parts of premix.

5. The feed containing astragalus polysaccharide according to claim 1, wherein The raw materials used in the basic diet include, by weight parts: 15 parts of corn, 7 parts of wheat middlings, 7 parts of corn bran, 10 parts of wheat bran, 2.5 parts of soybean oil, 10 parts of soybean meal, 3 parts of rapeseed meal, 6 parts of distillers grains, 5 parts of corn germ meal, 0.5 parts of artemisia annua powder, 9 parts of rice hull powder, 7 parts of peanut shell powder, 5 parts of white rod powder, 5 parts of alkali grass, and 3 parts of premix.

6. The feed containing astragalus polysaccharide according to claim 1, wherein Per kilogram of the premix includes: Fe 50 - 100 mg, Cu 10 - 30 mg, Zn 50 - 100 mg, Mn 5 - 15 mg, Co 0.1 - 0.2 mg, I 0.1 - 0.3 mg, Se 0.2 - 0.3 mg, VA 9000 - 11000 IU, VD 800 - 1000 IU, VE 30 - 70 mg, VK 1 - 3 mg, VB1 1 - 3 mg, VB2 4 - 8 mg, VB5 30 - 70 mg, VB6 1 - 3 mg, VB 12 0.01 - 0.03 mg, VB3 30 - 70 mg, VB9 30 - 60 mg, VB4 900 - 1100 mg and VB7 0.1 - 0.3 mg.

7. The feed containing astragalus polysaccharide according to claim 6, characterized in that, Per kilogram of premix includes: Fe 70 mg, Cu 20 mg, Zn 70 mg, Mn 10 mg, Co 0.15 mg, I 0.2 mg, Se 0.25 mg, VA 10000 IU, VD 900 IU, VE 50 mg, VK 2 mg, VB1 2 mg, VB2 6 mg, VB5 50 mg, VB6 2 mg, VB 12 0.02 mg, VB3 50 mg, VB9 44 mg, VB4 1000 mg and VB7 0.2 mg.

8. The preparation method of the feed containing astragalus polysaccharide according to claim 1, characterized in that, It includes the following steps: Mix the raw materials evenly in proportion to obtain the feed containing astragalus polysaccharide.

Images