35564 results about "Microbiology" patented technology

The invention relates to the technical field of green plant image recognition, in particular to a green plant disease recognition and early warning management system and method. The method comprises the following steps: acquiring a high-definition image of a green plant; performing image preprocessing on the green plant high-definition image to generate a standard green plant high-definition image; performing green plant part image segmentation and integration on the standard green plant high-definition image to obtain a standard green plant part image; performing part multi-dimensional disease feature extraction on the standard green plant part image to obtain green plant part multi-dimensional disease features; performing scab area identification on the standard green plant part image based on the multi-dimensional disease features of the green plant part to generate scab area identification data of the green plant part; and performing green plant disease type discrimination on the standard green plant part image according to the green plant part scab area identification data to generate green plant disease type discrimination data. According to the method, high-definition image processing, multi-dimensional feature extraction, propagation trend analysis and dynamic early warning are introduced, so that the accuracy and reaction time sequence of green plant disease recognition early warning are improved.

The present disclosure provides water-soluble film packages composed of a water-soluble film, where the packages include: one or more compartment(s) including dehydrated microbes. The disclosure further provides methods of production of the water-soluble film packages, and methods of application thereof. The water-soluble packages of the disclosure enable convenient and accurate application of microbial dry powders to plants, promote uniform dispersion of powdered microbes, enhance microbial shelf life, and enhance the survival of microbes on seed, while being environmentally sustainable.

The present invention relates to the technical field of pesticides, and discloses a biological agent-based rice sheath blight disease efficient control agent, which comprises an active flora, a carrier, an auxiliary agent and a disease resistance enhancing factor, the active flora is used for combining humidity, pH and temperature response type materials so as to make the bacterial agent dynamically released, and the disease resistance enhancing factor is used for controlling the disease resistance of the rice sheath blight disease. The carrier is used for enabling the active ingredients to be slowly released in a field environment and keep stability, the auxiliary additive is used for improving the activity, adhesiveness and soil adaptability of the fungicide, and the disease-resistant enhancing factor is used for expanding and improving the adaptability of the control agent. The ratio of the active flora to the carrier to the auxiliary additive to the disease-resistant enhancing factor is 10: 50: 13: 27. Through the synergistic effect of various functional strains and the combination of the dynamic release carrier, the occurrence and propagation of the rice sheath blight disease are effectively inhibited, the prevention and treatment rate can reach 75-85%, and the prevention and treatment effect is obviously higher than that of a traditional chemical pesticide or a single fungicide.

The invention relates to the technical field of microbial fermentation control, in particular to an automatic microbial reaction fermentation control system and a control method. The method comprises the following steps: collecting real-time microbial environment parameters and identifying a data multi-axis structure, extracting master control environment interval data, eliminating redundant signals to generate standard microbial environment data, defining a fermentation degree contrast template through fermentation historical records, and constructing a microbial fermentation timeline framework. The method comprises the following steps: acquiring standard environmental data, identifying a fermentation state of the standard environmental data, constructing a simulated microbial environmental field by using the standard microbial environmental data, performing global microbial reaction simulation, determining fermentation reaction structure partitions based on the data, performing difference comparison to generate fermentation difference data, and performing compensation simulation by using the difference data. And automatic fermentation compensation control of microorganisms is realized. According to the invention, the intelligent automatic control and optimization of the microbial fermentation process are realized, the fermentation efficiency and stability are obviously improved, and the manual intervention is reduced.

The invention relates to a fluorescent protein selection marker staygold-levEr gene as well as a construction method and application thereof, and belongs to the technical field of biological engineering. The invention provides a fluorescent protein selection marker staygold-levEr gene for screening an L-valine production strain and a construction method of a gene fragment, compared with other similar gene selection markers, the selection marker provided by the invention has the advantages that the fluorescence intensity is positively correlated with the concentration of L-valine, and the screening marker has the advantages that the screening marker can be used for screening the L-valine production strain; according to the screening marker, the screening efficiency of the L-valine high-yield strain can be obviously improved, the screening time and cost can be saved, the screening efficiency can be improved, and the screening marker has a wide application prospect.

The invention provides a genetically engineered bacterium, a preparation method thereof and application of the genetically engineered bacterium in synthesis of sclareol. The genetically engineered bacterium comprises a CcLPPS gene derived from Cistanus crenatus, and a CcLPPS gene derived from Cistanus crenatus, the genetically engineered bacterium is selected from yarrowia lipolytica. According to the invention, the stably expressed sclareol efficient de novo synthesis strain is constructed, and no organic reagent or any path intermediate substance is added in the whole fermentation process, so that the production cost is greatly reduced, and the subsequent separation and purification steps are simplified. The engineering yarrowia lipolytica strain provided by the invention is high in production intensity and simple in fermentation process, has a relatively good application prospect, and lays a foundation for producing sclareol by a microbial fermentation method.

The invention relates to serratia marcescens XJ108 and application thereof, and belongs to the technical field of microorganisms. The serratia marcescens XJ108 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.29557, the preservation date is January 10, 2024, and the address of the preservation institution is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The invention also provides application of the serratia marcescens XJ108 in acid environment, alkaline environment, high salinity environment and high chemical fertilizer environment soil tolerance and plant growth promotion. The serratia marcescens XJ108 provided by the invention has certain biological safety; meanwhile, the plant growth promoting function is achieved; and various extreme soil environments can be tolerated.

The present invention relates to the technical field of water-based coatings, and specifically to a PHA water-based coating, a preparation method and application thereof. The PHA water-based coating comprises the following raw materials: a PHA water-based dispersion, an emulsifier, a thickener, a film-forming aid and a leveling agent; wherein the PHA water-based dispersion is obtained by a PHA fermentation broth through a wall-breaking treatment. The present invention directly prepares the PHA water-based coating by using the PHA water-based dispersion obtained by fermentation and wall-breaking treatment upstream, and can prepare a PHA water-based coating that is not easy to settle, has good storage stability, is uniformly dispersed and has a small particle size.

The invention relates to the technical field of biology, in particular to a verticillium dahliae virulence gene, a verticillium dahliae virulence protein and application. The invention discloses a verticillium dahliae virulence gene VdPHO23like. The verticillium dahliae virulence gene VdPHO23like comprises a polynucleotide sequence for coding an amino acid sequence of SEQ ID NO: 3. According to the invention, expression of the gene is inhibited in a targeted manner through an RNA interference mediated gene silencing technology so as to weaken virulence of pathogenic bacteria, and an efficient, specific and environment-friendly comprehensive prevention and control strategy is provided for verticillium wilt of crops such as cotton.

The invention provides a plant lactobacillus capable of promoting mineral absorption and transport and application of the plant lactobacillus. The invention provides a plant lactobacillus of which the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.32404. The invention also provides a culture, a sterilized solution, a supernatant, a filtrate, a diluent, a precipitate or freeze-dried powder, a composition and a probiotic preparation containing the plant lactobacillus, and related applications and methods thereof.

The invention relates to the technical field of animal feed preparation, in particular to a functional feed additive for ruminants and a preparation method of the functional feed additive for the ruminants, and the functional feed additive for the ruminants is characterized by consisting of the following components in percentage by mass: probiotics which are saccharomycetes, bacillus subtilis and enterococcus faecalis; an enzyme preparation, xylanase and protease; prebiotics are added; a microcapsule coating material; a binder; the balance is a carrier; the probiotics and the enzyme preparation are wrapped in the chitosan / sodium alginate composite film through a microcapsule coating technology, adverse factors such as temperature, humidity and illumination in the production, transportation and storage process can be effectively isolated, meanwhile, in the rumen low-pH, strong-protease and complex-microorganism environment, the coating layer can slowly release and protect active ingredients, and the effect of preventing and treating the probiotics and the enzyme preparation is achieved. Effective content and activity during final feeding are ensured, consistent growth promotion can be kept in different varieties, weights and feeding stages, and production benefits and feeding stability are improved.

The invention discloses a broad-spectrum biocontrol strain water bacillus barren ZG18 and application thereof, and belongs to the technical field of plant disease control, and the broad-spectrum biocontrol strain water bacillus barren ZG18 is characterized in that the water bacillus barren ZG18 is water bacillus barren and is preserved in the China General Microbiological Culture Collection Center, and the preservation number of the water bacillus barren ZG18 is CGMCC (China General Microbiological Culture Collection Center). The preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, China; the preservation date is September 6, 2024; the preservation number is CGMCC (China General Microbiological Culture Collection Center) The biocontrol bacterium strain disclosed by the invention has a relatively strong inhibition effect on pathogenic fusarium sporotrichum, fusarium graminearum, botrytis cinerea, rhizoctonia solani and corticosis cucurbitae, the prevention and treatment effect of the strain ZG18 on tobacco target leaf spot reaches up to 51.34%, the prevention and treatment effect of the strain ZG18 on tobacco damping-off in a pot experiment is 65.91%, and the strain ZG18 has a promoting effect on tobacco growth.

The invention provides a technical method for improving the anti-aging effect of eurotium cristatum tea compound fermentation liquor, and belongs to the technical field of microorganisms, the Latin name of eurotium cristatum is Eurotium cristatum, the eurotium cristatum is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC M 2024643. According to the method, the yeast seed solution is inoculated into the tea-sea buckthorn leaf culture solution fermented by the eurotium cristatum, so that the content of anti-aging active components such as procyanidine B2, quercitrin, cyanidin-3-glucoside, nicotinic acid, naringenin and rutin in the fermentation culture solution can be remarkably increased through co-fermentation, and the elastase inhibition rate can be remarkably increased; the anti-aging effect of a component system of the fermentation culture solution is enhanced. The prepared tea-hippophae rhamnoides leaf compound fermentation liquor is suitable for development of anti-aging cosmetics and has a wide market prospect.

The invention discloses a preparation method of a curing agent with biological self-healing capability, which comprises the following steps: preparing a bacillus pasteurii bacterial solution, preparing the bacillus pasteurii bacterial solution into an endophytic spore solution, immersing polyvinyl alcohol fibers into a sodium alginate / endophytic spore solution, taking out the polyvinyl alcohol fibers, immediately immersing the polyvinyl alcohol fibers into a calcium acetate solution, drying the polyvinyl alcohol fibers to obtain core fibers with a hydrogel coating, and curing the core fibers with the hydrogel coating to obtain the curing agent with biological self-healing capability. The preparation method comprises the following steps: taking magnesium phosphate cement as a raw material, immediately immersing the magnesium phosphate cement into a chloroform solution of a polystyrene and polylactic acid polymer blend, drying to obtain a coated microbial liquid, premixing urea and glucose, doping the premixed urea and glucose into the magnesium phosphate cement, uniformly stirring, adding the coated microbial liquid, and uniformly stirring to obtain the product. According to the invention, phosphate cement is adopted as the curing agent, has the advantages of high strength, short setting time, strong durability, low alkalinity, good biocompatibility and the like, and compared with the curing agent in the prior art, the curing agent not only has a good curing effect, but also has a crack self-healing function, and is especially suitable for reinforcing foundation soil.

A system, method, and article for delivering an antimicrobial agent into the lumen of a trans-dermal catheter are disclosed. In an embodiment, the system comprises an elongate member configured for insertion into a lumen of a catheter, and the elongate member containing an antimicrobial. An antimicrobial agent can be placed on an interior surface of a retaining ring.

The invention provides an application of a fermentation lactobacillus mucus strain A21196 in preparation of a product for resisting anxiety and improving memory and concentration, and belongs to the technical field of biological medicines. Animal experiments show that the fermented lactobacillus mucus strain A21196 has the effects of resisting anxiety, resisting depression, improving cognitive impairment function and improving memory concentration, and provides a new means for preventing and / or treating nerve diseases related to anxiety disorder, depression and cognitive impairment.

The invention discloses a system and method for identifying microbial contamination in food and cosmetics based on AI identification, and the method comprises the steps: collecting to-be-detected food or cosmetics as a detection sample, and obtaining the bacterial colony growth state of microorganisms in the detection sample; the method comprises the following steps: acquiring a microbial growth image through microscopic imaging equipment, and preprocessing the microbial growth image to obtain a target area of a microbial colony; constructing an AI recognition model by adopting a convolutional neural network, and obtaining the species and quantity of microorganisms in the detection sample; parameters of the AI recognition model are adjusted in real time according to the types and the number of the microorganisms, a preset microorganism limited standard threshold value is combined, a visual analysis report is generated, the AI recognition model is combined with a Faster R-CNN target detection method, precise classification and number statistics of the types of the microorganisms are achieved, a complete microorganism pollution analysis report is formed, and the method is suitable for popularization and application. And the accuracy and scientificity of the final identification result are improved.

The invention discloses a method for detecting probiotics in fermented rotten vegetable feed based on the Internet of Things, and relates to the technical field of microbiological detection, and the method comprises the following steps: obtaining complete echo signal time sequence data, and carrying out high-frequency sampling on an echo signal time sequence by a sliding time window with a fixed length, and constructing a three-dimensional optical signal transient feature vector. According to the method, the transient feature vector of the three-dimensional optical signal is constructed, and a disturbance residual error and time sensitive weight mechanism is introduced, so that the abnormal disturbance of the optical signal is accurately recognized; by combining the optical interference index and the dynamic sampling window adjustment strategy, the refractive index mutation area is automatically avoided, the sensing misjudgment and data distortion are avoided, the detection stability and accuracy of the probiotic activity state in the fermentation environment are remarkably improved, and the intelligent monitoring capability of the feed quality is enhanced.

The invention discloses low-temperature-resistant and high-salt-resistant aerobic pseudomonas kirschner kirschner K2 as well as a preparation method and application thereof. The preparation method comprises the following steps: carrying out low-temperature and high-salt enrichment culture; screening and domesticating, and monitoring the concentration of ammonia nitrogen; and after 3-5 times of enrichment culture, diluting the enrichment culture, further culturing until a macroscopic bacterial colony is obtained, and then picking and carrying out plate streaking to separate a pure strain. The invention also relates to application of the strain in preparation or prevention of nitrification and denitrification nitrogen removal drugs in treatment of high-salt nitrogen-containing wastewater. The strain is low-temperature-resistant and high-salt-resistant heterotrophic-aerobic denitrifying bacteria, has the characteristic of low temperature resistance, grows well at low temperature and high salt salinity, has higher heterotrophic nitrification-aerobic denitrification nitrogen removal capacity, is suitable for nitrogen removal treatment of high-salt wastewater such as food processing wastewater and landfill leachate, and has wide application prospects. The superiority is particularly obvious under a low-temperature condition.

The invention discloses a probiotic with the depression improving effect and a microecological preparation and application thereof, and is characterized in that the probiotic comprises lactobacillus mucus PC17 with the preservation number of CGMCC No.33866, the active ingredients of the lactobacillus microecological preparation comprise thalli of the lactobacillus mucus PC17, and a preparation method of the microecological preparation comprises the following steps: adding the lactobacillus mucus PC17 into the lactobacillus mucus PC17, uniformly mixing the lactobacillus mucus PC17, the lactobacillus mucus PC17, the lactobacillus mucus PC17, the lactobacillus mucus PC17, the lactobacillus mucus PC17, the lactobacillus mucus PC17 and the lactobacillus mucus PC17 The lactic acid bacteria are inoculated into a fermentation culture medium according to a volume ratio of 1-5% to prepare the lactic acid bacteria microecological preparation, can be used for preparing antidepressant drugs, and has the advantages of being capable of promoting RIN14B cells to secrete 5-hydroxytryptamine and 5-hydroxytryptophan, reducing the levels of interleukin-1beta, interleukin-6, tumor necrosis factor-alpha, corticosterone and corticotropin in serum, and improving the antidepressant activity of the antidepressant drugs. The levels of aminobutyric acid and brain-derived neurotrophic factors in serum are improved, and the depression-like behaviors of CUMS depression model mice are obviously improved.

The invention discloses thermophilic fungus cleavable polysaccharide monooxygenase and application thereof, and belongs to the technical field of enzyme engineering and microbial engineering. The thermophilic fungus lysing polysaccharide monooxygenase provided by the invention has excellent thermal stability; when the lysable polysaccharide monooxygenase is used for synergistically hydrolyzing phosphoric acid swelling cellulose, microcrystalline cellulose and bagasse, the enzymolysis rate is increased, and the problems of poor thermal stability of the lysable polysaccharide monooxygenase, low enzymolysis rate of synergic cellulase and high LPMOs enzyme dosage are solved. The method has high practicability and can be widely applied to the enzymolysis process of various cellulosic substrates, so that the enzymolysis efficiency of the cellulosic substrates is remarkably improved.

The invention discloses a calendula double-bacterium fermentation product as well as a preparation method and application thereof, and relates to the technical field of plant fermentation. The preparation method of the calendula double-bacterium fermentation product disclosed by the invention comprises the following steps: carrying out primary fermentation by using lactobacillus plantarum, then carrying out secondary fermentation by using saccharomyces cerevisiae, and carrying out sterilization and filtration treatment on the calendula fermentation liquid subjected to secondary fermentation, so as to obtain the calendula fermentation liquid. The calendula fermentation liquor is subjected to combined fermentation through lactobacillus plantarum and saccharomyces cerevisiae, and the content of polyphenol and flavone in the calendula double-bacterium fermentation product is remarkably increased. Meanwhile, the calendula double-bacterium fermentation product has the effects of preserving moisture, resisting oxidation, relieving and controlling oil, and has a wide application prospect in the field of cosmetics.

Provided are an environmentally-friendly biological liquid fertilizer and a preparation method therefor. The environmentally-friendly biological liquid fertilizer comprises the following components in parts by weight: 95-105 parts of a microcapsule microbial inoculant liquid fertilizer, 10-15 parts of an amino acid fertilizer, 8-12 parts of glucose, 7-10 parts of potassium humate, and 400-500 parts of water, wherein the microcapsule microbial inoculant liquid fertilizer is a suspension formed by a microbial inoculant microcapsule in an inorganic fertilizer solution, and the microbial inoculant microcapsule is a hydrophobic microcapsule prepared by coating a microbial inoculant with sodium lignin sulfonate-corn starch. By means of preparing the hydrophobic microcapsule, the technical effect of coating a microbial inoculant to avoid an environmental impact is achieved, a variety of microbial inoculants are separately encapsulated and are dispersed in the inorganic fertilizer solution, and an organic fertilizer and a carbon source are supplemented to prepare the composite biological liquid fertilizer, thereby achieving the technical effects of easy absorption of the fertilizer, prevention against microbial antagonism, an increased survival rate and water-fertilizer integration.

The invention discloses cured beef and a preparation method thereof, and belongs to the technical field of fermented food. Comprising the following steps: trimming and dicing beef, adding seasoning spices, and adding a microbial agent for fermentation, the microbial agent is prepared from lactobacillus plantarum NR1-7 and aroma-producing yeast. According to the invention, the cured beef fermented by adding the Chinese prickly ash extracting solution, the lactobacillus plantarum NR1-7 and the aroma-producing yeast is relatively high in viable count, good in color and luster, rich in free amino acid quantity, moderate in protease activity and appropriate in organic acid content, and the pH value is stabilized to be about 5.5. Compared with natural fermentation, the growth and reproduction of inoculated dominant bacteria can accelerate the degradation of beef components and generate rich flavor, the pepper extract can ensure the complete beef structure by weakening the activity of endogenous enzyme, and in addition, the sour taste can be alleviated, the excessive degradation of fat can be relieved, and the fermentation quality of the cured beef can be improved.

The invention belongs to the technical field of medicinal and edible Chinese herbal medicine alcoholic drink, and particularly relates to lactobacillus plantarum and application thereof in fermentation of ginsenoside. The lactobacillus plantarum is specifically lactobacillus plantarum L100, and the preservation number of the lactobacillus plantarum L100 is CGMCC (China General Microbiological Culture Collection Center) No. 34425. The lactobacillus plantarum L100 has the function of converting and preparing the rare ginsenoside, basic components of ginseng can be directionally converted into high-value rare ginsenoside (such as Rg3, Rg5 and Rh1), and particularly, the lactobacillus plantarum L100 has extremely high specificity on generation of F5 and Rh10. Ginseng fermentation liquor obtained by fermenting ginseng through lactobacillus plantarum L100 and extracting solutions of medicinal and edible components such as codonopsis pilosula, astragalus membranaceus, liquorice and cistanche deserticola are used for preparing the liqueur, and a liqueur product with potential effects in the aspects of oxidation resistance, inflammation resistance, immunoregulation and the like is obtained.

The invention discloses a method for denitrifying wastewater by using a low-temperature-resistant heterotrophic nitrification and aerobic denitrification bacterium, and relates to a method for denitrifying wastewater by using a biological bacterium, the bacterium is Pseudomonas putida, is named as Pseudomonas umsongensis BK17, belongs to Pseudomonas, and is preserved in the China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC M 20242924, and the preservation date is December 27, 2024. The strain is a heterotrophic nitrification-aerobic denitrification bacterium, can efficiently remove inorganic nitrogen pollution in a water body, still has good denitrification performance under low-temperature stress, and is high in low-temperature tolerance. The strain can efficiently treat nitrogen-containing wastewater under the condition of 10 DEG C, and the removal efficiency can reach 99% or above. The method has great potential in the application of inorganic nitrogen pollution treatment of domestic sewage and other water bodies in cold regions in China.

Provided herein are methods, compositions, and kits for determining the spatial location of target nucleic acids, including endogenous and exogenous target nucleic acids, in a biological sample using padlock probes and substrates with spatially barcoded capture probes. Also disclosed herein are methods for determining a presence and / or location of a microbe (e.g., archaea, fungi, bacteria) in a biological sample, e.g., by determining the presence and / or location of a microbial target nucleic acid in the biological sample.

The invention belongs to the technical field of microbiology and agricultural application thereof, and particularly relates to talaromyces sp., a microbial agent and application of the talaromyces sp. According to the invention, a fungus with antagonistic action on fusarium oxysporum is screened from the root of black nightshade, the fungus is identified as talaromyces, the inhibition rate of the strain on fusarium oxysporum is 68.4%, and pot experiment shows that the relative control effect of the strain on tobacco root rot is 61.1%; a field experiment result shows that the relative prevention effect of the strain on the tobacco root rot is 88.8%, the disease index of the tobacco root rot can be effectively reduced, large-area infection of pathogenic bacteria is inhibited to a certain extent, and meanwhile, the talaromyces sp. Strain screened by the invention can induce the activity of defense enzymes PPO, PAL and POD of tobacco seedlings, improve the disease resistance of the seedlings and protect the growth of tobacco plants. Therefore, talaromyces sp. Has great potential value in the aspect of preventing and treating tobacco root rot.

The present disclosure is drawn to methods of utilizing nucleic acid barcodes and corresponding amplifying sites in cells in which the barcodes naturally occur. These barcodes and amplifying sites are reconfigured into a single nucleic acid cassette that provides for ease of use in tagging particular species, strains, or variants of cells, each with a different barcode. These barcodes can be used to track the colonization capabilities of the barcoded cells. The present disclosure further provides for assays that utilize natural barcodes to measure relative microbial colonization ability of a plant root system.

The invention relates to the technical field of genetic engineering, and particularly discloses a genetic engineering bacterium for producing melanin as well as a construction method and application of the genetic engineering bacterium. According to the application, the yield of the melanin can be effectively increased by modifying a metabolic pathway of the genetically engineered bacterium, and the yield of the melanin reaches 6.2 g / L and 18.5 g / L respectively through shake-flask culture and 5L fermentation tank culture. Compared with the prior art, the yield of melanin produced by the genetically engineered bacterium is improved by 30% at the shake flask fermentation level, the fermentation yield of a fermentation tank is improved by 4.3 times, in addition, expensive tyrosine is changed into glucose as a fermentation precursor substance, the production cost is greatly reduced, and an efficient and feasible solution is provided for large-scale production of melanin.