The invention discloses a hormone hydrogel rooting film, which is prepared by the steps of: swelling polyvinyl alcohol at normal temperature for 30min, and heating for completely dissolving polyvinyl alcohol to obtain a transparent liquid; cooling, adjusting the pH value of the solution with concentrated hydrochloric acid, adding glycerol and reacting to prepare a hydrogel liquid; and adding hormone to the hydrogel liquid and stirring. When in use, the hormone hydrogel rooting film is spread on the surface of a hard branch cutting shoot, so that a gel film is formed on the surface of the hard branch cutting shoot above the ground so as to achieve the purpose of reducing the moisture of the cutting shoot from dissipation, and a gel film is formed on the surface of the hard branch cutting shoot under the ground so as to keep a moisture state in soil and play a role of slowly releasing hormone to stimulate the rooting of the cutting shoot, thus the rooting critical stage of the hard branch cutting shoot is prolonged and the rooting survival rate of the hard branch cutting shoot is improved. The technical scheme disclosed by the invention is simple and convenient, high in raising seedling survival rate and significant in economic and social benefits, and saves seedling-raising investment by more than 10%.