The application discloses a transparent partially deacylated
gellan gum and a preparation method thereof, and belongs to the technical field of post-
processing of
gellan gum fermentation liquor. The method comprises the following steps: diluting high-acyl
gellan gum fermentation liquor to a
polysaccharide content of 0.8-1.1%; adding
sorbitol and buffer salt to adjust the pH to alkaline, adding
lysozyme and
protease to remove
bacteria, proteins and to perform mild partial deacylation; adjusting the pH to terminate the deacylation, then reducing the
viscosity at high temperature, and removing insoluble impurities through high-temperature and high-speed
centrifugation; adding low-level
alcohol to flocculate after the temperature is lowered and the pH is adjusted, and the finished product is obtained through
solid-liquid separation,
drying and crushing. The application solves the problem of clarification caused by high
viscosity and high gel temperature, the prepared product has good transparency, a gel temperature of 50-60 DEG C, excellent gel elasticity, stable performance, and can be widely applied to food, daily chemical and pharmaceutical fields such as
plant soft and hard capsules with high requirements on transparency, and fills the blank of commercial production of transparent partially deacylated gellan gum.