751 results about "Fructose" patented technology

The invention discloses a biomarker for evaluating litchi quality and a detection method, and relates to the technical field of biology. The marker is an SNP (Single Nucleotide Polymorphism) molecular marker, and the nucleotide sequence of the marker is as shown in SEQ ID NO.1-3. The molecular marker disclosed by the invention can be used for evaluating the quality of litchis, particularly evaluating the fructose content. Meanwhile, when the gene is applied to molecular marker assisted selection (MAS) and genome selection (GS), the genetic improvement progress of the litchi variety can be accelerated.

The invention discloses a yarrowia lipolytica engineering strain with high yield of resveratrol as well as construction and application of the yarrowia lipolytica engineering strain, and belongs to the technical field of synthetic biology and metabolic engineering. The invention provides a yarrowia lipolytica engineering strain. A 4-coumaric acid-CoA ligase gene, a multi-copy resveratrol synthase gene, a transketolase 1 gene, a fusion gene of a histidine phosphate aminotransferase gene and a tyrosine ammonia lyase gene, a glycolytic pathway hexokinase gene, a 6-phosphofructokinase-1 gene and a phosphoglycerate kinase gene are integrated, so that the recombinant protein is obtained. According to the present invention, by using glucose as the substrate, the high yield of resveratrol can be achieved by using glucose as the substrate, the resveratrol yield during shake-flask culture fermentation can achieve 3.157 g / L, the resveratrol yield can be increased to 30.7 g / L during 5L-scale fed-batch fermentation, and the resveratrol yield can be increased to 30.7 g / L when the resveratrol yield is increased to 30.7 g / L when the resveratrol yield is increased to 30.7 g / L when the resveratrol yield is increased to 30.7 g / L; the maximum value is reported by the yarrowia lipolytica system at present.

The invention belongs to the technical field of health-care food, and particularly relates to a sacha inchi oil-based gel candy with functions of reducing hypertension, hyperglycemia and hyperlipidemia and a preparation method thereof, and the sacha inchi oil-based gel candy comprises the following components: 1) a main component: 30-70% of sacha inchi oil; 2) a blood fat reducing synergistic component which is one or a combination of more of 0.5%-5% of phytosterol, 0.1%-2% of a red yeast rice extract and 0.01%-0.5% of sugarcane octacosanol; 3) a hypoglycemic synergistic component, which comprises one or a combination of more of 5%-20% of inulin / fructo-oligosaccharide, 0.5%-5% of a bitter gourd extract, 0.5%-5% of a mulberry leaf extract and 10-200 [mu] g / grain of chromium; 4) an antihypertensive synergistic component, wherein the antihypertensive synergistic component is one or a combination of more of 500-5000FU / grain of nattokinase, 0.5-5% of a celery seed extract and 1-8% of a hawthorn extract; through scientific compounding, the sacha inchi oil and various synergistic components exert a combined effect, and meanwhile, auxiliary regulation is carried out on blood sugar, blood fat and blood pressure; the main components are derived from natural plant extracts or functional grease, so that the health-care product is high in safety and suitable for daily health care.

The invention discloses a method for impregnating coffee beans with fructose under the assistance of ultrasonic waves, and relates to the technical field of food processing, and the method is technically characterized by comprising the following steps: selecting Robursta coffee beans with the moisture content of 10-12%; mixing with a 5% fructose solution according to a solid-liquid ratio of 1: 4, treating for 5-20 minutes (preferably 5 minutes) under ultrasonic waves of 40 kHz and 120 W, and totally impregnating for 4 hours; performing forced air drying at 40 DEG C for 23h until the water content is 10- And baking at the temperature of 175 DEG C for 7-8 minutes. A honeycomb net structure is formed on the surface of the coffee beans through ultrasonic treatment, the total sugar content is remarkably increased to 54.65 mg / g, amino acid composition is affected, and bioactive substances such as polyphenol and flavone are increased. The score of the baked coffee beans is more than 72 (the score of the coffee beans is 74.92 after the coffee beans are subjected to ultrasonic treatment for 5 minutes) through an SCA cup test, and the coffee beans have caramel fragrance, nut fragrance and smoother mouth feel. The method is simple and convenient to operate, low in cost and capable of effectively improving the flavor quality of the Robursta coffee.

The invention relates to the technical field of functional food, in particular to immune regulation type prebiotic syrup with synergy of galactomannan. The active components of the composite solid beverage are prepared from the following raw materials in percentage by weight: 20%-35% of fructo-oligosaccharide, 10%-18% of galactooligosaccharide, 8%-15% of stachyose, 3%-7% of xylooligosaccharide, 5%-12% of lactitol, 5%-10% of galactomannan, 2%-5% of yeast beta-glucan and 1%-3% of N-acetylglucosamine. The composition further comprises 4-8% of inulin and 3-6% of resistant dextrin as a matrix filling and slow-release carrier, and the sum of the weight percentages of all the active ingredients and the carrier is 100%. By remodeling intestinal microecology and inhibiting inflammation pathways, the intestinal inflammation state can be remarkably improved, expression of intestinal mucosa tight junction protein is promoted, and an innovative solution is provided for people suffering from chronic bowel diseases and immune imbalance.

The invention relates to the technical field of prebiotics preparation, and discloses a preparation method of prebiotics applied to reproduced areca catechu, and the preparation method comprises the following steps: carrying out enzyme catalysis reaction on sucrose by adopting fructosyl transferase to prepare fructo-oligosaccharide; according to the method, beta-galactosidase is adopted to carry out enzyme catalysis reaction on lactose, so that galactooligosaccharide is prepared; respectively carrying out decoloration, ion exchange, nanofiltration concentration and spray drying on the candy oligosaccharide and the galactooligosaccharide to obtain powder; the method comprises the following steps: mixing fructo-oligosaccharide powder and galactooligosaccharide powder according to a ratio, and uniformly distributing the mixture in a reproduced areca-nut matrix by adopting a spray-drying blending or low-temperature vacuum mixing mode to form a functional reproduced areca-nut product; according to the invention, low-temperature drying is carried out on the product after blending or vacuum mixing, so that the prebiotic areca nuts are released along with saliva in the chewing process, and are selectively utilized by bifidobacteria and lactic acid bacteria after entering the stomach and intestine, thereby achieving the purposes of regulating intestinal flora and improving the health-care function.

The invention belongs to the technical field of biology, and particularly relates to bifidobacterium longum subsp.infantis FMBL B250020GXY for metabolizing breast milk oligosaccharide and application of the bifidobacterium longum subsp.infantis FMBL B250020GXY, the bifidobacterium longum subsp.infantis FMBL B250020GXY is preserved in China Center for Type Culture Collection on September 1, 2025, and the preservation number is CCTCC M 20251931; three kinds of breast milk oligosaccharides, namely 2 '-fucosyllactose (2'-FL), lactose-N-neotetraose (LNnT) and lactose-N-tetraose (LNT), can be metabolized; plant source carbohydrates such as fructo-oligosaccharide and synanthrin can be metabolized; the compound has a relatively strong capability of removing DPPH and ABTS free radicals; a good inhibition effect is achieved on common pathogenic bacteria; good tolerance to acid and cholate is achieved; the strain is sensitive to gentamicin, streptomycin, tetracycline, chloramphenicol and vancomycin; the compound can be used for preparing antioxidant products, medicines for inhibiting pathogenic bacteria, infant foods and fermented foods, and has a wide application prospect.

The invention relates to an instant composition with a long-acting oral health-care function for dogs and cats as well as a preparation method and application of the instant composition, and belongs to the technical field of pet food and pet oral care. The composition comprises 20%-30% of whey protein powder, 10%-20% of hydrolyzed gelatin, 1%-3% of low-molecular-weight chitin saccharide gum, 1%-2% of modified cassava starch, 2%-5% of sodium alginate, 1%-3% of fructo-oligosaccharide, 0.03%-0.08% of lysozyme, 0.03%-0.08% of tea polyphenol, 0.05%-0.15% of zinc gluconate, 0.05%-0.1% of persimmon tannin, 3%-5% of tuna hydrolysate, 4%-6% of whole goat milk powder, 2%-4% of skim yoghurt powder and the balance water. The added active ingredients have long-acting slow-release and continuous effects in the formula, oral pathogenic bacteria can be effectively inhibited, dental plaque and dental calculus formation can be reduced, halitosis can be lastingly relieved, and the toothpaste has good market competitiveness.

The invention belongs to the technical field of biological pharmacy, and provides an application of jonquil glucoside in preparation of a medicine for treating a metabolism-related fatty liver disease, and the application is that the jonquil glucoside is applied to preparation of the medicine for treating the metabolism-related fatty liver disease. The treatment effect of the jonquil glycoside on MASLD is evaluated by adopting a method for establishing a fatty liver model through induction of HFD high-fat, high-fructose and high-cholesterol feed. Results show that the jonquil glucoside can reduce the liver weight by inhibiting weight gain, reduce the content of TC and TG in serum, improve the conditions of liver inflammation and fat change, reduce the oxidative stress level of liver tissue and restore SIRT1 protein expression of the liver tissue to achieve the purpose of treating MASLD.

The invention is applicable to the technical field of biology, and provides a method for rapidly extracting plant rhizome extracellular vesicles from monosaccharide and compound enzyme. According to the method, the plant extracellular vesicles are extracted by an enzymolysis-monosaccharide solution extraction coupling method for the first time, and the defect that an extraction method is single is overcome. According to the method, the conditions are mild, the primary property, the integrity and the biological activity of the vesicle structure are kept, the extraction purity is high, and the particle size distribution is uniform and concentrated in the range of 20-300 nm. The total protein extraction amounts of glucose, galactose and fructose solutions are respectively increased by 36.09% + / -1.18%, 37.65% + / -2.83% and 36.83% + / -2.59% compared with those of PBS (Phosphate Buffer Solution) extraction treatment. In addition, the'single ultracentrifugation 'purification process overcomes the problem of time consumption of traditional gradient centrifugation, and the time is shortened by more than 3h compared with that of a traditional extraction method. The method breaks through the technical bottleneck of large-scale production of the plant extracellular vesicles, and has great application and popularization values.

The invention discloses a preparation method of a sulfonic acid type covalent organic framework material and a preparation method of 5-hydroxymethylfurfural. The material is prepared by carrying out covalent condensation reaction on a monomer containing a sulfonic acid group precursor and a connecting ligand of a skeleton through a hot solvent method, and the catalyst has the characteristics of high crystallinity, large specific surface area, ordered pore structure, high sulfonic acid group density and uniform distribution. The preparation method is simple, convenient, efficient, mild in condition and high in yield. Compared with a traditional lewis acid catalyst for preparing 5-hydroxymethylfurfural through fructose dehydration, the material has the advantages of being uniform and adjustable in porosity, higher in active site, capable of promoting fructose hydrolysis and the like, efficient conversion can be achieved, side reactions and product transition polymerization are inhibited, and better industrialization prospects are shown.

The invention belongs to the technical field of preparation of bio-based platform compounds, and discloses a method for preparing 5-hydroxymethylfurfural by photocatalysis of glucose with a composite membrane, which comprises the following steps: by taking the composite membrane as a catalyst, mixing a glucose aqueous solution and a solvent under illumination conditions, and carrying out catalytic reaction to obtain crude 5-hydroxymethylfurfural production liquid; the method is used for solving the problems of low conversion rate, more byproducts, difficulty in continuous preparation, poor water resistance, strong corrosion and the like of the synthesis of 5-hydroxymethylfurfural. According to the method, a glucose solution passes through a composite membrane assembled by a mesoporous high-entropy sulfide membrane and a microporous zeolite molecular sieve membrane in a continuous flow manner, is efficiently isomerized into fructose under the action of illumination, and is dehydrated and continuously converted into 5-hydroxymethylfurfural. The composite membrane used in the method is high in water resistance, clean and non-toxic, has double functions of reaction and separation, effectively prevents the 5-hydroxymethylfurfural from further reacting with substances such as water and the like to generate humus, and realizes high-selectivity and continuous preparation of the 5-hydroxymethylfurfural.

The invention discloses a composite plant polypeptide composition with the effects of repairing pancreas and reducing blood sugar. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 10 to 50 parts of ginsenoside, 5 to 30 parts of radix astragali seu hedysari extract, 3 to 20 parts of radix rehmanniae polysaccharide, 2 to 15 parts of radix trichosanthis alkaloid, 5 to 25 parts of rhizoma dioscoreae polysaccharide, 5 to 30 parts of bitter gourd polypeptide, 3 to 20 parts of folium mori alkaloid, 8 to 40 parts of rhizoma polygonati polysaccharide, 5 to 25 parts of fructus hippophae flavone, 10 to 35 parts of wheat germ peptide, 2 to 15 parts of coptis berberine, 10 to 45 parts of corn stigma polysaccharide and 3 to 20 parts of saussurea involucrata polyphenol. 1-10 parts of sophora flavescens total alkaloids, 5-25 parts of a dendrobium extract, 5-30 parts of a radix puerariae extract, 8-35 parts of L-arabinose and 10-40 parts of small molecule peptides; 20 to 100 parts of collagen peptide and 15 to 80 parts of albumin peptide; 5-50 parts of microcrystalline cellulose, 3-30 parts of fructo-oligosaccharide and 0.5-5 parts of magnesium stearate; the limitation of single sugar control or simple component mixing in the prior art is broken through, a synergistic system of plant active components and functional polypeptides is constructed, and the purity and retention rate of the plant active components are improved by optimizing the extraction process.

The invention relates to polygonatum cyrtonema polysaccharide and application thereof. The novel polysaccharide PRP is extracted and purified from polygonatum cyrtonema and is neutral inulin type polysaccharide. The PRP takes a node alpha-D-glucose-(22)-beta-D-fructose similar to cane sugar as a center. The C6 site of glucose has an additional beta-fructose (2-> 6) substituent, and two chains extend out of the core through a (2-> 1)-beta-fructose bond to form a Y-type moderate branch structure. Compared with other selenium nanoparticles with stable polysaccharides, the selenium nanoparticles coated with polygonatum polysaccharide provided by the invention have smaller initial particle size, more negative zeta potential, stable batch repeatability and excellent long-term stability.

The invention belongs to the technical field of biochemistry, and particularly relates to a probiotic composition for relieving depression as well as a preparation method and application of the probiotic composition. The probiotic composition is prepared from bacillus cecum, lactobacillus reuteri and an oligosaccharide composition; wherein the viable count of the bacillus cecum and the lactobacillus reuteri is 10 < 9 >-10 < 11 > CFU / g; the oligosaccharide composition is prepared by compounding polydextrose, fructo-oligosaccharide and galactooligosaccharide according to an optimized weight ratio. According to the invention, the oligosaccharide composition is cooperated with the probiotics to play a curative effect, so that the proliferation effect of the bacteroides cecum and the lactobacillus reuteri in the intestinal tract is improved, and the balance of the probiotics in the intestinal tract is regulated. Detection results of behavioristics and physiological indexes of mice show that the depression-like behaviors of the mice are remarkably reduced under the action of the composition, and various biochemical indexes are remarkably improved; the composition has a certain improvement effect on depressive mood.

The invention relates to the field of probiotic fermentation, and discloses a probiotic fermented beverage prepared from sea buckthorn fruit peptide and morinda citrifolia fruit peptide and a preparation method thereof, and the probiotic fermented beverage is prepared from the following components by weight: 10-15 parts of a sea buckthorn fruit peptide extract, 8-12 parts of a morinda citrifolia fruit peptide extract, 5-8 parts of a composite probiotic starter, 3-5 parts of fructo-oligosaccharide, and 1-2 parts of a natural sweetener. According to the present invention, the sea buckthorn fruit peptide and the morinda citrifolia fruit peptide are scientifically compounded, such that the synergistic interaction of the two plant active components is achieved, the double-fruit peptide matrix provides the differentiated nutrition supply for the composite probiotics, and the fermentation efficiency is improved through the nutrition complementation; according to the present invention, with the fermentation medium, the growth requirements of different strains are met, the proliferation efficiency and the metabolic activity of the probiotics are significantly improved, the industry problems of poor strain adaptability and low viable count in the traditional single-substrate fermentation are solved through the innovative compatibility, and the fermentation process is stable and efficient.

The invention belongs to the technical field of traditional Chinese medicines, mainly relates to a preparation method of herbal ointment, and particularly relates to a preparation method and application of herbal ointment for treating qi depression. The formula of the herbal paste comprises the following components in parts by weight: 70-80 parts of lily bulbs, 40-50 parts of lotus seeds, 70-80 parts of poria cocos, 40-50 parts of malt, 70-80 parts of wheat, 45-50 parts of cape jasmine fruits, 70-80 parts of rose flowers, 70-80 parts of dried orange peel, 40-50 parts of purple perilla, 30-40 parts of licorice roots, 10-15 parts of xylitol, 15-20 parts of fructo-oligosaccharide and 20-25 parts of isomaltooligosacharide. The lily bulbs, the lotus seeds, the poria cocos, the wheat and the malt are subjected to combined fermentation treatment through bifidobacterium animalis subsp. Lactis CICC-21715 and saccharomyces boulardii BR-288, and the fructus gardeniae is subjected to enzymolysis treatment. The herbal paste prepared by the invention has the effects of relieving depression and anxiety, eliminating nodules and cysts, promoting intestinal peristalsis and improving sleep.

The invention discloses a composition capable of quickly dispelling the effects of alcohol and relieving moderate and mild fatty liver and application of the composition, and particularly relates to the technical field of health care products. The composition comprises the silybum marianum extract, the schisandra chinensis extract, the globe artichoke extract and the 1, 6-fructose diphosphate and cyclodextrin-clathrated silybum marianum extract as raw materials, on one hand, the bioavailability of the product is improved, the taste of the product is better, and the taste of the clathrated product is improved due to the fact that silybin is very bitter and cyclodextrin has a shielding property; and on the other hand, the FDP and the three plant extracts have a synergistic effect, and the combined composition can achieve the effects of quickly dispelling the effects of alcohol and relieving moderate and mild fatty liver.

The invention provides a probiotic composition for inhibiting helicobacter pylori as well as a probiotic agent and application thereof, and belongs to the technical field of probiotic functional products. The probiotic composition is prepared from the following components in parts by weight: 0.05 to 2 parts of a pediococcus pentosaceus strain A21358, 0.01 to 1.5 parts of a plant lactobacillus strain A21136, 0.05 to 1.5 parts of a lactobacillus rhamnosus strain A21149 and 0.03 to 1.2 parts of a lactobacillus reuteri strain A21325. The probiotic agent comprises the probiotic composition, resistant dextrin, xylooligosaccharide, erythritol, inulin, fructo-oligosaccharide, hericium erinaceus powder, fucoidin and at least one fruit powder selected from grape powder, passion fruit powder and kiwi fruit powder, wherein the specific weight parts of the resistant dextrin, the xylooligosaccharide, the erythritol, the inulin, the fructo-oligosaccharide, the hericium erinaceus powder, the fucoidin and the at least one fruit powder are selected from grape powder, passion fruit powder and kiwi fruit powder. The probiotic agent has the effect of inhibiting helicobacter pylori, and a new thought is provided for clinical related auxiliary treatment of helicobacter pylori infection.

This invention relates to the field of chemical synthesis technology and discloses a method for preparing 2,5-furandicarboxylic acid from fructose, comprising the following steps: preparing reaction system A containing fructose, an organic bromide, a solid acid catalyst, and water; performing a dehydration reaction to separate the solid acid catalyst and obtain a fructose dehydration reaction solution; preparing reaction system B containing the fructose dehydration reaction solution, a co-oxidant, an oxidation catalyst, and acetic acid; performing an oxidation reaction under an oxidizing gas atmosphere; separating the product to obtain 2,5-furandicarboxylic acid. This invention utilizes a co-oxidant, which can reduce the requirement for fructose concentration in the one-pot preparation of 2,5-furandicarboxylic acid while ensuring the yield and purity of FDCA, thereby improving production efficiency. Furthermore, it reduces or even eliminates the use of hydrobromic acid in the oxidation reaction, thus reducing corrosion to equipment.

The invention discloses a macrobrachium rosenbergii probiotic biofloc technology (BFT) culture system construction method based on an SBOS-FOS composite carbon source, and belongs to the technical field of aquaculture. The total ratio of SBOS (soybean oligosaccharide) and FOS (fructo-oligosaccharide) in the composite carbon source is 2%-10%, and the balance is glucose; and adding the composite carbon source into a macrobrachium rosenbergii culture system according to the C / N ratio of (10: 1)-(20: 1) according to the feed protein content in the culture water body. Through a specific ratio of SBOS to FOS, the content of functional probiotics in a culture water body is specifically increased, stable control of ammonia nitrogen and nitrite nitrogen and directional optimization of a carbon source-flora-host three-level regulation and control network in a BFT system are realized, the weight gain rate (WG), the specific growth rate (SGR) and digestive enzyme activity (trypsin and amylase) of macrobrachium rosenbergii are increased, and the survival rate of the macrobrachium rosenbergii is increased. The abundance of probiotics in the water body and the immune gene expression of the macrobrachium rosenbergii are obviously improved.

The invention relates to the technical field of solid beverages, in particular to isomaltitol dietary fiber particles and a preparation method thereof, and the isomaltitol dietary fiber particles comprise the following components: isomaltitol, Chinese date mulberry extract, golden flower Tibetan tea powder, fructo-oligosaccharide, polydextrose, xylooligosaccharide, stachyose, insoluble dietary fiber, inulin, konjac glucomannan and lycium barbarum polysaccharide. By processing the raw materials and optimizing the process, the problems of poor taste, low stability, single function and the like of the existing dietary fiber product are solved. The application of the microencapsulated isomaltitol and the ultrahigh pressure treated inulin respectively realizes the slow-release control of a sweetening agent and the improvement of the solubility of polysaccharide fibers, and the flavor adjustment of the Chinese date mulberry extract and the decaffeinated golden flower Tibetan tea powder, so that the product has high dietary fiber content and also has fresh and sweet fruit fragrance and elegant tea fragrance; and the taste is fine and caking-free.

The application discloses a D-allulose 3-epimerase mutant, a preparation method and application thereof, and relates to the fields of genetic engineering and enzyme engineering. The amino acid sequence of the D-allulose 3-epimerase mutant is shown as SEQ ID NO. 1. The specific enzyme activity of the D-allulose 3-epimerase mutant provided by the application is increased from 10.21 U / mg of a control (before mutation) to 15.29 U / mg at 80 DEG C and pH 6.0; and the equilibrium conversion rate is increased from 29.98% of the control (before mutation) to 36.26% when the substrate is 700 g / L of fructose. The D-allulose 3-epimerase mutant provided by the application has higher catalytic activity, and has a very broad application prospect in the efficient production and preparation of D-allulose.

The invention relates to the technical field of gene engineering, in particular to application of a knockout SlABCB13 gene in increasing the soluble sugar content of tomato fruits. The function of the SlABCB13 gene is deleted by mediating gene editing, so that the content of soluble sugar (cane sugar, fructose and glucose) and the content of soluble solids in tomato fruits are remarkably increased, and the SlABCB13 participates in sugar transport of the tomato fruits. Meanwhile, the mutant does not influence related yield indexes such as plant height, stem diameter, fruit size, fruit setting number and single-plant fruit yield, and a new thought is provided for the problem that yield and quality are difficult to consider in existing tomato breeding.

The invention discloses selenium functionalized clostridium butyricum-bacillus subtilis composite probiotic freeze-dried powder as well as a preparation method and application thereof. The compound probiotic freeze-dried powder is prepared from the following components in parts by mass: 20 to 30 parts of compound probiotic bacterial sludge, 10 to 20 parts of trehalose, 20 to 30 parts of fructo-oligosaccharide, 20 to 30 parts of dried skim milk, 8 to 10 parts of sodium alginate and 1 to 2 parts of calcium chloride. The preparation method comprises the following steps: blending selenium-functionalized clostridium butyricum, bacillus subtilis and a freeze-drying protective agent in sodium alginate hydrogel, and further performing vacuum freeze-drying to obtain the composite probiotic freeze-dried powder. The freeze-dried powder prepared by the invention continuously releases hydrogen through metabolism of probiotics, shows excellent active oxygen scavenging ability, and shows outstanding application value in the field of intestinal injury treatment. The method disclosed by the invention is simple and efficient in process and has good potential of large-scale production.

The application discloses application of VvERF105 and VvSWEET15 promoter interaction in reducing sugar accumulation in fruits, and belongs to the technical field of biotechnology, and particularly relates to application of VvERF105 and VvSWEET15 promoter interaction in reducing sugar accumulation in fruits.The protein VvERF105 can interact with the VvSWEET15 promoter, the expression amount of the VvERF105 is regulated, the contents of grape glucose and fructose in grape fruits are negatively regulated, and the application prospect is good in the field of plant breeding.

The invention belongs to the technical field of gene engineering, and in particular relates to a D-psicose-3-epimerase mutant subjected to Spytag / SpyCatcher cyclization modification and an application of the D-psicose-3-epimerase mutant. The preparation method comprises the following steps: carrying out multi-site simultaneous mutation on 109th aspartic acid and 160th serine of wild type D-psicose-3-epimerase to obtain a mutation intermediate, respectively connecting a Spytag tag and a SpyCatcher tag to the N end and the C end of the mutation intermediate, and carrying out cyclization modification, so as to obtain the wild type D-psicose-3-epimerase, wherein the Spytag tag and the SpyCatcher tag are respectively connected to the N end and the C end of the mutation intermediate; when fructose is used as a raw material to produce D-psicose and host bacteria are escherichia coli, the reaction of catalyzing 600 g / L fructose to produce D-psicose is performed for 4 h, and the conversion rate can reach 35.7%; when the host bacteria are bacillus subtilis, the reaction for catalyzing 500 g / L fructose to produce D-psicose is performed for 4 h, and the conversion rate reaches 36.5%; compared with a wild type D-psicose-3-epimerase strain of bacillus subtilis, the conversion rate of the strain is improved by 13.5%.

1. Name of the designed product: packing box (milk fruit sugar oral solution). 2. Use of the designed product: for product packing. 3. Design points of the designed product: combination of shape and pattern. 4. Picture or photo showing the design points: perspective view 1.

The invention relates to the field of traditional Chinese medicine beverages, and discloses a winter traditional Chinese medicine beverage with a lung moistening function and a preparation method thereof.The winter traditional Chinese medicine beverage is prepared from, by weight, 8-12 parts of lily extract, 5-8 parts of almond peptide, 2-4 parts of mogroside, 3-5 parts of tremella polysaccharide, 0.5-1 part of pericarpium citri reticulatae volatile oil and 1-2 parts of bulbus fritillariae cirrhosae slow-release microcapsules. The invention relates to a low-temperature enzymolysis and supercritical CO2 extraction combined decoction, which is prepared from the following raw materials in parts by weight: 4-6 parts of a compound probiotic leavening agent, 3-5 parts of natural honey, 2-3 parts of fructo-oligosaccharide, 0.3-0.8 part of citric acid, 0.1-0.3 part of a natural flavor regulator and the balance of purified water, aims at the problem of extensive traditional decoction process, and realizes accurate treatment of different medicinal material characteristics through a low-temperature enzymolysis and supercritical CO2 extraction combined technology. According to the present invention, the high temperature is prevented from damaging the thermosensitive component, the extraction efficiency of the insoluble substance is improved through the pressure regulation, the retention rate of the active component is qualitatively improved, and the composite probiotic fermentation process is innovatively introduced in the systematicness of the compatibility.

The invention relates to a process for the production of a mixture of 5-hydroxymethylfurfural and glucose from a feedstock containing fructose in the presence of at least one polar aprotic solvent and of at least one dehydration catalyst, in which process the reaction temperature is less than 90° C.