717results about "Unicellular algae" patented technology

The invention discloses a method for constructing a multicellular living body material by adopting a droplet bioreactor, which belongs to the crossing field of biological materials and microbial engineering, and comprises the following steps: (1) resuspending different cells in different polymer solutions to prepare degradable or phase-separable hydrogel particles containing cells; (2) dispersing the hydrogel particles in a cross-linkable hydrogel matrix to form a composite hydrogel; and (3) curing the composite hydrogel, and then carrying out degradation treatment to obtain the liquid cavity structure multicellular system bioreactor with spatial partition and dynamic interaction characteristics. According to the invention, ordered distribution and metabolism synergy of different microorganisms in space can be realized, controllable degradability and excellent mass transfer performance are realized, bacterial slow release and metabolite exchange can be maintained, and the method is suitable for long-term stable operation of a continuous flow reactor. The method has wide application prospects in the fields of green biological manufacturing, renewable energy source production, environmental restoration, biomedical engineering and the like.

The invention relates to the technical field of shellfish breeding, in particular to a rapid microalgae breeding method for shellfish breeding based on a photobioreactor and a cement pool. The method comprises the following steps: firstly, optimizing a photobioreactor structure and an illumination layout, controlling a culture environment by matching with a multi-parameter regulation and control system, breeding microalgae seeds with high resistance and high carbon sequestration capacity, culturing to a preset density, and partially harvesting and inoculating into a cement pond, after partial harvest, the photobioreactor realizes continuous culture by supplementing disinfected seawater and nutritive salt; the harvested high-density microalgae are inoculated into a cement pond, nutritive salt is supplemented in a doubled mode, air and carbon dioxide mixed gas is used for continuous aerated culture, and the microalgae are promoted to rapidly reach the index growth period and used for being directly fed to shellfish larvae; according to the rapid microalgae breeding method for shellfish seedling culture, the yield and quality of microalgae are both considered, the success rate and productivity of aquatic seedling culture are effectively improved, the production cost and the labor intensity of workers are reduced, and the method is suitable for large-scale popularization.

The invention discloses engineering chlamydomonas reinhardtii for synthesizing high-value pigment and a construction method and application thereof.An engineering chlamydomonas reinhardtii strain Blup-BKT-CHYB-CHLC is preserved in the China General Microbiological Culture Collection Center on September 1, 2025, and the preservation number is CGMCC NO.46634. The content of astaxanthin in the constructed engineering chlamydomonas reinhardtii strain reaches 25.5 mg / L, and the content of astaxanthin in the engineering chlamydomonas reinhardtii strain reaches 9.5 mg / L; in addition, the light energy utilization efficiency is remarkably enhanced, the stress resistance is improved, very strong oxidation resistance is achieved, the accumulation of astaxanthin endows the engineering strain with highlight tolerance, the yield of high-value pigments is increased, and the biomass of the engineering strain is greatly superior to that of a wild strain under the highlight condition. In addition, the chlorophyll c synthase is applied to the reconstruction of a pigment system of the chlamydomonas reinhardtii for the first time, a frontier tool is quickly converted, and a molecular mechanism of'chlorophyll c-astaxanthin-blue pigment 'for synergistically regulating the photosynthetic efficiency of the chlamydomonas reinhardtii and product synthesis is clarified for the first time. The research blank of pigment system coupling regulation and control of photosynthesis and metabolism in the chlamydomonas reinhardtii is filled.

The invention discloses an algae species with both efficient denitrification and high free ammonia tolerance and application thereof. The invention provides an inflatedfruit senna herb which is preserved in China General Microbiological Culture Collection Center (CGMCC) on December 11, 2024, and the preservation number of the inflatedfruit senna herb is CGMCC No.46280. The inflatedfruit senna herb has the advantages that the inflatedfruit senna herb is preserved in the China General Microbiological Culture Collection Center (CGMCC); the inflatedfruit senna provided by the invention has efficient assimilation removal capability on NO3 <->-N, NO2 <->-N and NH4 < + >-N, and has relatively strong tolerance on high-concentration free ammonia (FA). A bacterial-algae symbiotic system is constructed by the inflator and activated sludge, and is coupled with a dynamic membrane process, so that the denitrification efficiency of the nitrogen-containing wastewater can be remarkably improved, the addition of exogenous organic matters, the aeration energy consumption and the N2O emission are reduced, meanwhile, high-value microalgae biomass is obtained, and efficient, stable and resourceful treatment of the nitrogen-containing wastewater is realized.

The invention discloses a construction method and application of a chlamydomonas reinhardtii-sourced high-sweetness protein variant engineering algal strain, the construction method comprises the following steps: carrying out gene modification on sweeteners Thaumatin and Brazzein, optimizing preference codons of chlamydomonas reinhardtii to synthesize CrThaumatin and CrBrazzein encoding genes, cloning the encoding genes into an expression vector pGM6, introducing the recombinant expression vector into a chlamydomonas reinhardtii wild-type chlamydomonas sp. Algal strain, and carrying out high-sweetness protein variant engineering algal strain of the chlamydomonas reinhardtii-sourced high-sweetness protein variant engineering algal strain of the chlamydomonas reinhardtii. And screening by using a paromomycin resistance marker carried by the expression vector to successfully obtain an engineering algal strain. According to the present invention, the engineering strain is subjected to fermentation production to obtain the dry and stable Chlamydomonas reinhardtii powder rich in the target sweet protein, and the protein purification oral test results show that the sweet taste of the Chlamydomonas reinhardtii powder is 3-5 times of the sweet taste of the natural Thaumatin and Brazzein protein;

The present disclosure relates to liquid (e.g., water-soluble) compositions comprising antimethanogenic compounds, and methods of administering the same to reduce enteric methane emissions from ruminant animals, and / or improve feed-efficiency. Certain disclosed compositions exhibit improved rapid effect over prior art compositions. The disclosure further relates to methods of concentrating antimethanogenic compounds, extracting antimethanogenic compounds, and methods of increasing the bioavailability of antimethanogenic compounds.

The invention discloses a method for improving the yield of microalgae SA-2 lipid. The invention relates to the technical field of microalgae biology, in particular to a method for increasing the yield of microalgae SA-2 lipid. The method comprises the following steps: 1, inoculating microalgae SA-2 into a BBM culture medium; 2, then inoculating to a BBM culture medium containing NaHCO3; and 3, inoculating into an-NBBM culture medium, and culturing. The microalgae SA-2 disclosed by the invention is an extreme saline-alkali-resistant algae strain separated from rhizosphere soil of salix mongolica in saline-alkali soil in Andam City, Heilongjiang Province, China, the NaHCOO tolerance concentration of the microalgae SA-2 is up to 800mM (mmol / L), and the microalgae SA-2 is a typical saline-alkali adaptive algae strain. In the prior art, a lipid enhanced culture method for the algal strains with saline-alkaline adaptability is not reported yet.

The present disclosure relates to a high purity PDRN extract derived from microalgae and a method for preparing the same. Specifically, the high-purity PDRN extract derived from microalgae according to the present disclosure uses microalgae, which can be massively cultivated at any time without being limited by the external environment or time, as a raw material, thereby facilitating production and having excellent sustainability, and is safe without the risk of human being infected by foreign viruses, and thus has a low molecular weight, and thus, when applied to products such as cosmetics, the PDRN extract can be easily produced. And the skin absorption rate is excellent.

An Akk11 strain-containing probiotic agent capable of relieving hyperuricemia, and the use thereof. Strains in the probiotic agent include an Akkermansia muciniphila Akk11 strain and a Lactobacillus johnsonii LJ09 strain. It is found in the present application that the two strains possess a potential interaction and can cooperate with each other, thereby synergizing the effect of relieving hyperuricemia, which is specifically manifested in: being capable of remarkably regulating uric acid metabolism and improving renal clearance functions; being capable of effectively inhibiting the activity of a key enzyme for uric acid synthesis so as to reduce the production of uric acid, and being capable of remarkably relieving renal damage and renal dysfunctions associated with high levels of uric acid.

To provide a novel free fatty acid-producing algae with excellent FFA productivity, and a method for producing free fatty acids using the same free fatty acid-producing algae. [Solution] Free fatty acid-producing algae in which either or both of the enzymes that convert acyl ACP to acyl aldehyde (AAR) and the enzyme that converts lipid aldehyde to alkane (ADO) are functionally impaired or functionally deficient, and further, free fatty acid-producing algae into which either or both of the katG gene and sodB gene have been introduced, and a method for producing fatty acids using these free fatty acid-producing algae.

The invention discloses a method for promoting biomass accumulation of haematococcus pluvialis, which comprises the following steps: adding salicylic acid, melatonin and alpha-ketoglutaric acid into a basic culture medium, and replacing ferric trichloride with ferric citrate as an iron source to obtain an improved BG-11 culture medium; the method comprises the following steps: inoculating a haematococcus pluvialis seed solution into a culture bottle containing an improved culture medium, carrying out illumination treatment on the first day to the second day by adopting pulsed red light with the wavelength of 620 nm in the early stage of a green stage, carrying out illumination treatment on the third day to the fifth day by adopting red light with the wavelength of 660 nm in the middle stage of the green stage, and carrying out illumination treatment on the sixth day to the seventh day by adopting pulsed red light with the wavelength of 680 nm in the later stage of the green stage. Through a synergistic scheme of the improved BG-11 culture medium, staged red light treatment and dynamic environment regulation and control, the problems of low light energy utilization rate and insufficient stress resistance in the existing haematococcus pluvialis culture process are solved, high efficiency and stability of haematococcus pluvialis biomass accumulation are realized, the biomass can reach 7.0 * 10 < 5 > cells / mL in the later period of the green stage, and the yield of the haematococcus pluvialis can reach 7.0 * 10 < 5 > cells / mL in the later period of the green stage. And the survival rate of haematococcus pluvialis cells reaches 95%.

The invention discloses a single-cell green alga and application thereof. The name of the single-cell green alga is Coelastrus sp. BS01, the preservation number is CCTCC NO: M 20251671, the preservation date is July 22, 2025, and the preservation unit is China Center for Type Culture Collection in Wuhan University, Wuhan, China. The single-cell green alga can grow in a selenium-polluted water body with relatively high concentration, can efficiently remove selenium in the water body and is enriched in alga cells, so that the single-cell green alga has the application potential of enriching and converting selenium.

The invention is applicable to the technical field of industrial wastewater treatment, and provides a construction method of a microalgae-in-situ bacteria strengthening system for degrading naphthenic acid, which comprises the following steps: separating and identifying two strains of in-situ bacteria from a discharge port of an oil-producing wastewater treatment plant, and constructing a three-strain typical stain-resistant green alga-in-situ bacteria co-culture system. The strengthening system can highly tolerate and efficiently degrade naphthenic acid, can effectively remove nitrogen and phosphorus, and has higher biomass and high-value compound yield. The microalgae-in-situ bacterium strengthening system can reduce pollutant discharge and improve the microalgae resource utilization rate, and the microalgae-in-situ bacterium strengthening system can be used as a biological strengthening degradation technology of naphthenic acid and can be suitable for purification treatment of oil production wastewater and petroleum polluted water areas.

The present disclosure relates to a high-purity PDRN extract derived from microalgae and a preparation method thereof. Specifically, the high-purity PDRN extract derived from microalgae according to the present disclosure is easy to produce and highly sustainable by using microalgae capable of being mass-cultivated at any time without external environmental or time constraints as a raw material, is safe due to no risk of human infection by exogenous viruses, and can also be excellent in skin absorption when applied to products such as cosmetics due to its low molecular weight.

The present invention relates to a method for decomposing peracetic acid using a metal compound and a method for culturing microorganisms using the decomposition method. The cultivation of microorganisms using the method of the present invention allows an effective removal of the peracetic acid used in a culture medium for sterilization.

An object of the present invention is to provide a formulation effective for activation of hair dermal papilla cells. Provided are a composition for activation of hair dermal papilla cells, a composition for hair regrowth promotion or hair growth, a composition for hair quality improvement, and a composition for FGF-2, FGF-7 or VEGF production promotion, containing a culture supernatant and / or extracellular vesicles of a microalga.

The present invention is related to the fields of bioplastics. In particular, a strain of photosynthetic cyanobacteria (i.e. blue-green algae) having a high growth rate was engineered to overexpress limonene synthase. These engineered cyanobacteria improve auto-sedimentation for biomass harvest and provide for plastic bioremediation capture. Further, machine-learning methods are provided for improving some of the aspects of cultivating cyanobacteria including, but not limited to, assessing real-time light availability and predicting real-time algae growth rates to increase a cyanobacteria biomass.

A living paint comprises a living photosynthetic microorganism, binder, growth medium, and water. The microorganism may comprise algae, cyanobacteria, or lichens. The binder may be composed of primary

The application discloses a kind of long-acting targeted cultivation alga high-density complex of water body and preparation method thereof, it is related to aquaculture field, the high-density complex includes the following weight parts components: small material premix 3-5 parts, sodium chloride 35-40 parts, gelatinized starch 1-3 parts, attapulgite 40-50 parts, cement 3-7 parts, titanate coupling agent 1-3 parts, molasses 2-4 parts and purified water 1-5 parts.The preparation method of the high-density complex includes the following steps: small material premix is subpackaged, is stirred uniformly with other components and adds molasses to carry out humidity control, then subpackaging and compression molding are formed, in turn plastic envelope, package and film winding.The long-acting targeted cultivation alga high-density complex of water body provided in the application can directional cultivation diatom and chlorella, and continuously release functional material, stabilize the abundance of beneficial algae, effectively solve the problem that directional cultivation beneficial organism cannot be carried out and functional material release speed is too fast.

The invention relates to the technical field of microbial culture, in particular to a heterotrophic culture method of benthic diatom, which comprises the following steps: S1, adding benthic diatom into an improved SE culture medium, and culturing for 5-7 days until OD540 is 0.8-1.2 to obtain a benthic diatom seed solution; according to the method disclosed by the invention, a nutrition system is constructed by taking waste glycerol as a carbon source and sodium alginate coated urea as a slow-release nitrogen source, and the benthic diatom, nitrogen-fixing bacteria and phosphate solubilizing bacteria are co-cultured on a porous ceramic carrier to be cultured under a dark condition, so that compared with the prior art, the method disclosed by the invention thoroughly gets rid of the dependence on illumination, and the yield is increased. The rapid, high-yield and stable heterotrophic culture of the benthic diatom is realized, meanwhile, the raw material and energy consumption cost is greatly reduced, and the resource recycling rate is improved; the obtained diatom biomass can be used for aquatic bait, biological energy, environmental restoration and development of high-added-value products, and has a wide industrial application prospect.

The present disclosure provides a Schizochytrium sp. Strain and its use thereof.

The present invention relates to the production of biocomposite materials through the co-cultivation of mycelium and algae. This innovative method integrates sustainable practices and utilizes waste materials to create carbon-negative, durable, and biodegradable composites suitable for various applications, including interior design, construction, automotive and packaging.

The invention discloses a method for evaluating the ecological risk of a perfluorinated compound based on the chlorophyll concentration of microalgae, which comprises the following steps: collecting a water body of a region to be evaluated, detecting the concentration of the perfluorinated compound in the water body, and obtaining a localized microalgae culture system at the same time; adding perfluorinated compound solutions with different concentrations into the localized microalgae culture system to form an experimental group, and setting a blank group without perfluorinated compounds; sampling and detecting the chlorophyll concentration of the microalgae in the experimental group and the blank group, and calculating the chlorophyll inhibition ratio of the microalgae; calculating the half effect concentration according to the function relationship between the concentration of the perfluorinated compound and the chlorophyll inhibition ratio of the microalgae; according to the concentration and the half effect concentration of the perfluorinated compounds in the water body, calculating to obtain a risk quotient for judging and evaluating the ecological risk of the perfluorinated compounds in the water body. According to the method, the half effect concentration, the mixed exposure effect and the risk quotient value are integrated to serve as ecological risk composite criteria, and the method has the advantages of being high in environmental correlation, high in sensitivity and high in indication performance.

It can be useful to regulate the growth of microbial cells. Some embodiments herein provide genetically engineered microbial cells that can produce bacteriocins to control the growth of microbial cells. In some embodiments, microbial cells are contained within a desired environment. In some embodiments, contaminating microbial cells are neutralized. In some embodiments, a first microbial cell type regulates the growth of a second microbial cell type so as to maintain a desired ratio of the two cell types.

Provided is a method for extracting a useful component from algae, the method being characterized by comprising the following steps (1a) and (2a) or the following steps (2b) and (1b). (1a) is a step for extracting a first useful component from dried algae by using a non-polar solvent. (2a) is a step for extracting a second useful component from the residue after the extraction in the step (1a) by using a solvent including a polar solvent. (2b) is a step for extracting a second useful component from dried algae by using a solvent including a polar solvent. (1b) is a step for extracting a first useful component from the residue after the extraction in the step (2b) by using a non-polar solvent.

A culture device by which microalgae can be satisfactorily cultured using a simple structure while suppressing an increase in energy consumption, and a culture method. A main body of this culture device comprises an accommodation part which accommodates contents and to which gas is supplied. The inner wall faces of the accommodation part are joined together to form a joined part which extends along the gas supply direction. A guide part and a circulation part, which are adjacent to each other across the joined part, are each along the extending direction of the joined part and communicated with each other via a guide part inlet and a guide part outlet. A gas supply port enables gas supply toward the guide part.

Methods of standardizing a fermentation process may include obtaining a fluidic sample, measuring one or more physical parameters of the sample, comparing the measurement of the physical parameter of the material to a baseline value of the physical parameter for the fermentation process, and responsive to a deviation of the measurement of the physical parameter from the baseline value, determining a remediation action based on a correlation between the physical parameter and regulatory genes of a fermentation organism.

The invention belongs to the technical field of biology, and particularly relates to a method for increasing the fucoxanthine accumulation amount by using microcystis subjected to depolymerization treatment to feed palmarthria formosana. According to the method, microcystis subjected to depolymerization treatment are added in the culture process of the palmitothecium for heterotrophic culture, meanwhile, continuous inflation is conducted, the microcystis subjected to depolymerization treatment are swallowed by the palmitothecium, and the higher fucoxanthine accumulation amount in the palmitothecium is obtained by means of the culture mode. The experiment proves that after the microcystis is subjected to pretreatment and depolymerization, the removal efficiency of the microcystis is higher by the brown algae, the limitation of treatment of harmful cyanobacteria and ingestion of protozoa is made up, and meanwhile, an integrated solution is provided for biological treatment of cyanobacterial bloom and resource utilization of the microalgae; meanwhile, a new way is provided for obtaining fucoxanthine with higher accumulation amount.

Provided herein are cells, enzymes, and methods for improved cannabinoid production.

Provided herein are vesicular nanocatalysts decorated on their surface with enzymes, such as for industrial use. More particularly, the nanocatalysts include outer membrane vesicles (OMVs) presenting on their surface carbonic anhydrase or a functional fragment or derivative thereof. Uses, methods of manufacture, and compositions of the nanocatalysts are also disclosed herein.