1417results about "Unicellular algae" patented technology

The invention discloses a Trichotoma sp. ZSL-NS2 strain capable of producing protein and application of the Trichotoma sp. ZSL-NS2 strain in wastewater treatment of a dairy farm. The Trichotoma sp. ZSL-NS2 strain named as the Trichotoma sp. ZSL-NS2 strain is preserved in the China Center for Type Culture Collection on November 7, 2024, and the preservation number is CCTCC NO.M20242469. The Trichotoma sp. ZSL-NS2 strain is named as the Trichotoma sp. ZSL-NS2 strain. The algal strain is separated from soil beside a wastewater storage pool of a dairy farm, is purified and enriched under a high-salt condition (the salinity is 2.0-5.0%), has relatively strong wastewater tolerance and pollutant purification capacity, has the removal rates of more than 70% of COD, 85% of ammonia nitrogen, 75% of TN and 95% of conventional pollutants respectively, and particularly has the most obvious removal effect on ammonia nitrogen and total phosphorus. In addition, the protein content of the conneronea is as high as 52%, and the conneronea can be used as a protein raw material for high-value application.

The invention relates to a carbon quantum dot for strengthening and promoting growth of microalgae and application thereof. In the process of preparing the carbon quantum dots by a conventional hydrothermal method, a precursor mixture is subjected to acidification modification in advance, the pH value is adjusted to 2 by using nitric acid, and then a hydrothermal reaction is performed, so that the prepared carbon quantum dots have the capability of promoting the growth of microalgae. However, if other acids are adopted or pH adjustment is not in place, the effect cannot be achieved, and even the inhibition effect can be achieved. The obtained product is good in effect and stable in performance, no special reaction device needs to be additionally arranged in the acidification modification process, and the method has the advantages that operation is easy and convenient, raw materials are easy to obtain, and the process can be quantified; the method is simple and convenient in process operation, has universality and is easy for industrial production.

The invention discloses a bacterial strain for degrading aspartame, a bacterial-algae symbiotic system and application of the bacterial strain and the bacterial-algae symbiotic system, the bacterial strain is corn sphingomonas zeae NCU J1, and the preservation number of the bacterial strain is CCTCC (China Center for Type Culture Collection) NO: M 20251462. And an algal-bacterial symbiotic system is constructed by adopting an oriented symbiotic combination of the corn sphingomonas NCU J1 and the chlorella sp. The corn sphingomonas NCU J1 can metabolize an artificial sweetening agent aspartame and degradation products thereof, metabolism complementation can be achieved through an algal-bacterial symbiotic interface, and bacteria preferentially promote aspartame to be subjected to photohydrolysis to generate degradation products such as diketopiperazine derivatives. The microalgae synchronously start isomerism enzymatic reaction to convert degradation products such as diketopiperazine derivatives into low-toxicity products, the risk of secondary pollution is reduced by 40% or above compared with that of a traditional physical and chemical method, 150 mg / L aspartame can be efficiently degraded within 2 days, generation of derivatives of aspartame can be greatly reduced, secondary pollution is reduced, and the method is suitable for industrial production. And the method has a good application prospect in the aspect of rapidly treating aspartame-containing wastewater.

The invention discloses a method for constructing a multicellular living body material by adopting a droplet bioreactor, which belongs to the crossing field of biological materials and microbial engineering, and comprises the following steps: (1) resuspending different cells in different polymer solutions to prepare degradable or phase-separable hydrogel particles containing cells; (2) dispersing the hydrogel particles in a cross-linkable hydrogel matrix to form a composite hydrogel; and (3) curing the composite hydrogel, and then carrying out degradation treatment to obtain the liquid cavity structure multicellular system bioreactor with spatial partition and dynamic interaction characteristics. According to the invention, ordered distribution and metabolism synergy of different microorganisms in space can be realized, controllable degradability and excellent mass transfer performance are realized, bacterial slow release and metabolite exchange can be maintained, and the method is suitable for long-term stable operation of a continuous flow reactor. The method has wide application prospects in the fields of green biological manufacturing, renewable energy source production, environmental restoration, biomedical engineering and the like.

The invention relates to the technical field of shellfish breeding, in particular to a rapid microalgae breeding method for shellfish breeding based on a photobioreactor and a cement pool. The method comprises the following steps: firstly, optimizing a photobioreactor structure and an illumination layout, controlling a culture environment by matching with a multi-parameter regulation and control system, breeding microalgae seeds with high resistance and high carbon sequestration capacity, culturing to a preset density, and partially harvesting and inoculating into a cement pond, after partial harvest, the photobioreactor realizes continuous culture by supplementing disinfected seawater and nutritive salt; the harvested high-density microalgae are inoculated into a cement pond, nutritive salt is supplemented in a doubled mode, air and carbon dioxide mixed gas is used for continuous aerated culture, and the microalgae are promoted to rapidly reach the index growth period and used for being directly fed to shellfish larvae; according to the rapid microalgae breeding method for shellfish seedling culture, the yield and quality of microalgae are both considered, the success rate and productivity of aquatic seedling culture are effectively improved, the production cost and the labor intensity of workers are reduced, and the method is suitable for large-scale popularization.

Apparatuses and methods for microscopic analysis of a sample by simultaneously characterizing infrared absorption characteristics of a plurality of spatially resolved locations are described herein. These apparatuses and methods improve sampling times while collecting microscopic data regarding composition of a sample across a wide field.

The invention belongs to the technical field of biological sewage treatment, and relates to a bacteria-algae particle preparation method and system for promoting bacteria-algae system granulation based on pressure response. The preparation method comprises the following steps: co-culturing the mixed microalgae cultured to a logarithmic phase and aerobic activated sludge in a photobioreactor, in the culture process, jointly regulating and controlling aeration, a carbon source and inlet and outlet water to promote granulation of a bacteria-algae system according to the principle of'pressure-induced stress response ', and controlling dissolved oxygen levels in different periods according to the principle of'unbalanced air supply' during aeration regulation and control; the carbon source regulation follows the principle of unbalanced nutrition to control the COD concentration in different time periods; the water inlet and outlet regulation follows the principle of reducing the settling time, and the water change settling time is gradually shortened in the culture period. The bacteria-algae granular sludge which is good in settling performance, high in dirt-removing power and compact in structure is prepared, the prepared bacteria-algae granular sludge is used for treating sewage and wastewater, pollution of carbon, nitrogen, phosphorus and the like can be efficiently solved, the effluent quality is effectively improved, and carbon emission reduction in the sewage treatment field is promoted.

The invention relates to the technical field of microalgae heterotrophic culture and biomass proliferation, in particular to a chlorella pyrenoidosa heterotrophic high-density proliferation method. The specific technical scheme is as follows: the heterotrophic high-density proliferation method of chlorella pyrenoidosa comprises the following steps: pre-culture and heterotrophic propagation of chlorella pyrenoidosa: inoculating a chlorella pyrenoidosa solution into a BG11 culture medium, and culturing for 3-4 days in a constant-temperature shaking table with the temperature of 25 + / -1 DEG C, the rotating speed of 200rpm and the light-dark ratio of 0h: 24h to obtain a primary culture chlorella solution; inoculating the primary culture algae liquid into a pre-sterilized and optimized heterotrophic culture medium, and carrying out expanding culture under the same culture condition for 3-4 days to obtain a secondary expanding culture algae liquid; (2) a heterotrophic high-density culture stage: regularly supplementing a nutrient culture medium containing high-concentration glucose, nitrate and phosphate by adopting an index fed-batch feeding strategy; other conditions are kept consistent during culture; and (3) an in-situ protein accumulation induction stage: when the chlorella pyrenoidosa reaches an exponential growth end stage, adding paclobutrazol into the system, and keeping other conditions consistent while keeping exponential fed-batch culture. According to the method disclosed by the invention, the biomass of the chlorella pyrenoidosa is increased by about 128.6% compared with that of the traditional batch culture, the protein content can reach 49.14%, meanwhile, the configuration of essential amino acids is improved, and the requirements of high-end biomass resource application are met. The method has the advantages of simplicity and convenience in operation and high production efficiency, and is suitable for the fields of high-quality protein extraction and related biomass production.

The invention belongs to the field of microalgae-fungus bioengineering and the technical field of lignin wastewater treatment, and discloses a method for synthesizing grease by utilizing lignin wastewater through microalgae-fungus co-culture, which comprises the following steps: (1) inoculating microalgae into a first culture medium to obtain a microalgae seed solution; the microalgae is chlorella; inoculating the fungal spores into a second culture medium, and carrying out shake cultivation to obtain fungal pellets, so as to obtain a fungal seed solution; the fungi are cunningham echinophora; preparing lignin wastewater, adding culture medium inorganic salt into the lignin wastewater, and sterilizing to obtain a lignin wastewater culture medium; and (2) co-inoculating the fungus seed solution and the microalgae seed solution into a lignin wastewater culture medium for co-culture to obtain the microalgae-fungus symbiont synthesized with grease. According to the method, the lignin wastewater is treated through co-culture of microalgae and fungi, removal of organic pollutants in the lignin wastewater and enrichment of lignin can be synchronously and effectively achieved, and the yield of grease is increased.

The invention relates to the technical field of microorganisms, and provides haematococcus pluvialis KM2-1 and application thereof. The invention provides a haematococcus pluvialis KM2-1 which is preserved in the China Center for Type Culture Collection on April 3, 2025, and the preservation number of the haematococcus pluvialis KM2-1 is CCTCC (China Center for Type Culture Collection) NO: M 2025704. The invention also provides an application of the haematococcus pluvialis KM2-1 in enrichment of carotenoids. The invention also provides an application of the haematococcus pluvialis KM2-1 in preparation of products rich in carotenoids. The haematococcus pluvialis KM2-1 provided by the invention has better tolerance to light stress, and can promote the accumulation of carotenoids under the condition of high-intensity light stress, so that the carotenoids are better enriched.

According to the haematococcus pluvialis strain and the application thereof, cell wall breaking operation can be carried out more easily when astaxanthin is extracted, astaxanthin can be extracted more effectively, and the cost of natural astaxanthin is reduced. The haematococcus pluvialis RMC16 strain provided by the invention has the preservation number of CCTCC (China Center for Type Culture Collection) NO: M 20241404. The haematococcus pluvialis mutant strain obtained by screening has the characteristics of thin wall, no secondary cell wall, high astaxanthin content and the like, the haematococcus pluvialis mutant strain is high in growth speed and can quickly reach a plateau phase, the individual proportion of parental cells in a population bred by the haematococcus pluvialis mutant strain serving as a parent is higher, the cost required by extraction of natural astaxanthin can be remarkably reduced, and the haematococcus pluvialis mutant strain is suitable for industrial production. The method is suitable for large-scale popularization.

The invention discloses 3D printing high-light-transmittance bio-ink, an artificial 3D algal-bacterial symbiont and a preparation method and application of the artificial 3D algal-bacterial symbiont. The 3D printing high-light-transmittance bio-ink is prepared from polyether F127 diacrylate, polyethylene glycol diacrylate, pyridine degradation special-effect bacterium paracoccus sp. NJUST47, chlorella sorokiniana FACHB-275, a light absorbent and a liquid culture medium containing a photoinitiator, and the 3D printing high-light-transmittance bio-ink is prepared into an artificial 3D bacterium-algae symbiont through one-step 3D printing. The 3D printing high-light-transmittance bio-ink has good synergistic pyridine degradation performance, the constructed artificial 3D algal-bacterial symbiont has high algal-bacterial loading capacity and excellent light utilization rate, enhanced removal of volatile toxic organic pollutant pyridine can be achieved, and degradation stability is good.

The invention provides a phycomycete symbiotic system capable of degrading microplastics as well as a construction method and application of the phycomycete symbiotic system, and belongs to the technical field of biological treatment of wastewater. The method comprises the following steps: mixing an algae mixture, aerobic activated sludge and microplastics, and carrying out enrichment culture to obtain an enrichment culture; screening and separating a part of the enrichment culture to obtain at least two pure algae species, and extracting the other part of the enrichment culture to obtain a bacterial filtrate; and mixing the pure algae species, the bacterial filtrate and the microplastics for algae-bacteria co-culture to obtain the algae-bacteria symbiotic system capable of degrading the microplastics. The phycomycete symbiotic system capable of degrading the microplastics is constructed by adopting the method disclosed by the invention, so that the time cost and the economic cost for culturing and obtaining germplasm resources are greatly reduced; and the phycomycete symbiotic system can perform in-situ degradation on the micro-plastics at normal temperature, and is simple to operate and convenient to popularize and use.

Algae harvesting and cultivating systems and methods for producing high concentrations of algae product with minimal energy. In an embodiment, a dead-end filtration system and method includes at least one tank and a plurality hollow fiber membranes positioned in the at least one tank. An algae medium is pulled through the hollow fiber membranes such that a retentate and a permeate are produced.

The invention provides a haematococcus pluvialis culture medium and a culture method thereof. The culture medium comprises a nutrition proliferation culture medium, a stress induction culture medium and an accumulation strengthening culture medium which are used for segmented culture, the nutrition proliferation culture medium comprises sodium nitrate, monopotassium phosphate, potassium chloride, ferric ammonium citrate, sodium citrate and a trace element mixed solution; adding an osmotic stress agent, an oxidation inducer and plant hormones into a stress induction culture medium; and accumulating and strengthening the culture medium to remove sodium nitrate, and adding and supplementing a carbon source, a polymer penetrant and a zwitterionic stabilizer. The culture method comprises the following steps: a nutrition proliferation stage, a stress induction stage and an accumulation strengthening stage. According to the method, through a three-section culture medium and a fine regulation culture process, and through the synergistic effect of a composite stress factor and a dynamic environment signal, on the premise of ensuring relatively high activity of algae cells, bidirectional synergistic efficient improvement of biomass growth and astaxanthin accumulation is realized.

The invention relates to the technical field of microorganisms, and provides Haematococcus pluvialis RXXHp1 which is preserved in the China Center for Type Culture Collection on May 19, 2023, and the preservation number of the Haematococcus pluvialis RXXHp1 is CCTCC NO: M 2023791. The invention also provides an application of the haematococcus pluvialis RXXHp1. The application comprises the step of enriching at least one of organic selenium and astaxanthin in a selenium ion stress environment by using the haematococcus pluvialis RXXHp1. The invention further provides algae powder containing the haematococcus pluvialis RXXHp1 and application of the haematococcus pluvialis RXXHp1 or the algae powder in preparation of wine. The haematococcus pluvialis RXXHp1 provided by the invention has certain tolerance to a selenium ion stress environment, not only can be used for enriching organic selenium, but also can be used for promoting the accumulation of astaxanthin; besides, when the haematococcus pluvialis RXXHp1 is used for preparing the wine, the content of active substances such as astaxanthin and xanthophyll in the wine can be remarkably increased, so that the effects of improving the color and flavor of the wine and improving the oxidation resistance of the wine are achieved.

The invention discloses an intelligent cooperative regulation and control method and system for dissolved oxygen in a haematococcus culture solution, relates to the field of bioengineering, and controls a decision-making layer to dynamically switch between a growth promotion regulation and control mode and a stress induction regulation and control mode based on biomass data and physiological status data in a haematococcus culture stage so as to realize cooperative regulation and control of dissolved oxygen, illumination, pH and a carbon source. According to the method, a growth promotion mode and a stress induction mode are dynamically switched, biomass accumulation is promoted through dissolved oxygen sine wave pulse fluctuation in the vegetative period, astaxanthin synthesis is induced through high-oxygen and low-oxygen circulation and blue light regulation in the stress period, and compared with traditional static regulation, the biomass is increased, and the yield of astaxanthin is increased. A light-oxygen synergistic factor k is introduced to dynamically adjust the fluctuation range of dissolved oxygen, the problem of illumination-dissolved oxygen mismatching is solved, and the photosynthetic efficiency is improved; a stress intensity index SI is established, stress parameters are adaptively optimized based on the astaxanthin synthesis rate, cell damage caused by over-stress is avoided, and the stability of the astaxanthin synthesis rate in the stress period is improved.

The invention discloses engineering chlamydomonas reinhardtii for synthesizing high-value pigment and a construction method and application thereof.An engineering chlamydomonas reinhardtii strain Blup-BKT-CHYB-CHLC is preserved in the China General Microbiological Culture Collection Center on September 1, 2025, and the preservation number is CGMCC NO.46634. The content of astaxanthin in the constructed engineering chlamydomonas reinhardtii strain reaches 25.5 mg / L, and the content of astaxanthin in the engineering chlamydomonas reinhardtii strain reaches 9.5 mg / L; in addition, the light energy utilization efficiency is remarkably enhanced, the stress resistance is improved, very strong oxidation resistance is achieved, the accumulation of astaxanthin endows the engineering strain with highlight tolerance, the yield of high-value pigments is increased, and the biomass of the engineering strain is greatly superior to that of a wild strain under the highlight condition. In addition, the chlorophyll c synthase is applied to the reconstruction of a pigment system of the chlamydomonas reinhardtii for the first time, a frontier tool is quickly converted, and a molecular mechanism of'chlorophyll c-astaxanthin-blue pigment 'for synergistically regulating the photosynthetic efficiency of the chlamydomonas reinhardtii and product synthesis is clarified for the first time. The research blank of pigment system coupling regulation and control of photosynthesis and metabolism in the chlamydomonas reinhardtii is filled.

Photobioreactors are described herein. The photobioreactors include a vessel for cultivating microalgae. The vessel defines an interior chamber comprising a culture containing medium. The vessel has at least one transparent wall to permit passage of light of a frequency necessary to promote growth of the microalgae in the culture containing medium. The vessel also includes a plurality of sensors, each sensor being configured to monitor at least one environmental condition within the photobioreactor, and at least one flow generator positioned within the vessel. The photobioreactor also includes a controller communicatively coupled to each of the plurality of sensors and the at least one flow generator. The controller is configured to: receive sensor data from each of the plurality of sensors, apply a mathematical model to the sensor data to determine when to adjust at least one environmental condition within the photobioreactor and adjust one or more components of the photobioreactor.

A Schizochytrium algae strain rich in n-3 fatty acid and a screening and culture method therefor. Compared with a conventional strain, the content of EPA and the content of n-3 DPA in the provided strain are greatly improved, and the strain has good genetic stability, fast growth rate and good industrialization prospects. Also provided are a culture medium for mutagenesis of Schizochytrium sp., a culture medium for screening Schizochytrium sp., and a suitable mutagenesis method and screening method.

The invention discloses an algae species with both efficient denitrification and high free ammonia tolerance and application thereof. The invention provides an inflatedfruit senna herb which is preserved in China General Microbiological Culture Collection Center (CGMCC) on December 11, 2024, and the preservation number of the inflatedfruit senna herb is CGMCC No.46280. The inflatedfruit senna herb has the advantages that the inflatedfruit senna herb is preserved in the China General Microbiological Culture Collection Center (CGMCC); the inflatedfruit senna provided by the invention has efficient assimilation removal capability on NO3 <->-N, NO2 <->-N and NH4 < + >-N, and has relatively strong tolerance on high-concentration free ammonia (FA). A bacterial-algae symbiotic system is constructed by the inflator and activated sludge, and is coupled with a dynamic membrane process, so that the denitrification efficiency of the nitrogen-containing wastewater can be remarkably improved, the addition of exogenous organic matters, the aeration energy consumption and the N2O emission are reduced, meanwhile, high-value microalgae biomass is obtained, and efficient, stable and resourceful treatment of the nitrogen-containing wastewater is realized.

The present disclosure relates to the field of microbial technology and provides a Haematococcus pluvialis KM2-1 and its applications. The present disclosure provides a Haematococcus pluvialis ( Haematococcus lacustris ) KM2-1, which was deposited at the China Center for Type Culture Collection on April 3, 2025, and its deposit number is CCTCC NO: M 2025704; the present disclosure also provides an application of Haematococcus pluvialis KM2-1 in enriching carotenoids; the present disclosure also provides an application of Haematococcus pluvialis KM2-1 in preparing products rich in carotenoids. The Haematococcus pluvialis KM2-1 provided by the present disclosure has good tolerance to light stress and can promote the accumulation of carotenoids under high-intensity light stress conditions, thereby better enriching carotenoids.

The invention discloses application of a CMIET protein and a coding gene thereof in improving manganese resistance of plants. The invention belongs to the technical field of biology, and particularly relates to application of CMIET protein and a coding gene thereof to improvement of manganese resistance of plants. The CMIET protein can regulate and control biological manganese resistance, and excessive manganese in Chlamydomonas chloroplast can be discharged to cytoplasm by regulating and controlling expression of the protein CMIET or regulating and controlling gene or regulating and controlling activity or content of the protein CMIET, so that balance of manganese ion concentration in the chloroplast is maintained, and the chloroplast is prevented from being affected by manganese toxicity; the normal photosynthesis capability of the cells is maintained, and the normal photosynthesis is ensured.

The invention discloses chlorella for efficiently removing eutrophic elements in sewage and application of the chlorella, and relates to the technical field of eutrophic sewage treatment. According to the invention, the chlorella sp. 111-4 is obtained through screening, and can efficiently remove eutrophic elements such as nitrogen, phosphorus and TOC in sewage; the chlorella sp. 111-4 is preserved in the China Center for Type Culture Collection, and the preservation number of the chlorella sp. 111-4 is CCTCC NO: M 2025475. The chlorella sp. 111-4 is inoculated into the sewage to be treated, the sewage treatment effect is more remarkable than that of other reported chlorella, and the degradation rate of nitrogen and phosphorus in the wastewater within 7 days reaches up to 97% or above.

The invention discloses a flocculation-controllable genetic engineering alga and a construction method and application thereof, and is characterized in that the genetic engineering alga is phaeodactylum tricornutum into which an enabling plasmid pPha-J47869-EG01992-eGFP-FSP is introduced, the FSP is directionally expressed on the outer side of a phaeodactylum tricornutum cell, and the construction method comprises the following steps: constructing an induced expression plasmid pPha-J47869-eGFP and a directional expression plasmid pPha-J47869-EG01992-eGFP; artificially synthesizing a codon-optimized FSP coding sequence, inserting the codon-optimized FSP coding sequence into the downstream of the eGFP of the directional expression vector, and constructing an enabling plasmid; finally, the enabling plasmids are electrically transformed into phaeodactylum tricornutum, an f / 2 plate culture medium is coated with the phaeodactylum tricornutum for culture, positive algae strains are screened and identified, and the gene engineering algae with controllable flocculation are constructed and have the advantages that the gene engineering algae can be efficiently combined with white carbon black, and the enabling process of microalgae is efficiently coupled with the culture and collection process of the microalgae.

The invention discloses a construction method and application of a chlamydomonas reinhardtii-sourced high-sweetness protein variant engineering algal strain, the construction method comprises the following steps: carrying out gene modification on sweeteners Thaumatin and Brazzein, optimizing preference codons of chlamydomonas reinhardtii to synthesize CrThaumatin and CrBrazzein encoding genes, cloning the encoding genes into an expression vector pGM6, introducing the recombinant expression vector into a chlamydomonas reinhardtii wild-type chlamydomonas sp. Algal strain, and carrying out high-sweetness protein variant engineering algal strain of the chlamydomonas reinhardtii-sourced high-sweetness protein variant engineering algal strain of the chlamydomonas reinhardtii. And screening by using a paromomycin resistance marker carried by the expression vector to successfully obtain an engineering algal strain. According to the present invention, the engineering strain is subjected to fermentation production to obtain the dry and stable Chlamydomonas reinhardtii powder rich in the target sweet protein, and the protein purification oral test results show that the sweet taste of the Chlamydomonas reinhardtii powder is 3-5 times of the sweet taste of the natural Thaumatin and Brazzein protein;

The present disclosure relates to liquid (e.g., water-soluble) compositions comprising antimethanogenic compounds, and methods of administering the same to reduce enteric methane emissions from ruminant animals, and / or improve feed-efficiency. Certain disclosed compositions exhibit improved rapid effect over prior art compositions. The disclosure further relates to methods of concentrating antimethanogenic compounds, extracting antimethanogenic compounds, and methods of increasing the bioavailability of antimethanogenic compounds.

The present disclosure relates to methods and systems for extending metabolism of algal biomass or enzyme extracts, including the production and extraction of algal secondary metabolites with pharmaceutical, industrial, and agricultural uses. Aspects of this disclosure relate to production of antimethanogenic compounds from algae and other organisms. The disclosure further relates to compositions comprising antimethanogenic compounds and methods of using the same to reduce enteric methane emissions from ruminate animals. Methods of culturing algae in solutions with oil layers and triggering release of secondary metabolites through stress are also described.

The invention belongs to the technical field of microorganisms, and particularly relates to transgenic chlamydomonas capable of secreting antibacterial peptide outside cells as well as a construction method and application of the transgenic chlamydomonas. Specifically, the invention provides transgenic chlamydomonas capable of secreting antibacterial peptide outside cells. Tests prove that not only can the water quality be improved and the immunity of fishes be improved, but also the step of purifying the antibacterial peptide protein can be effectively omitted. The zebra fish experiment is carried out, and it is proved that the survival rate of the zebra fish can be increased. Meanwhile, compared with an escherichia coli and yeast expression system, the chlamydomonas reinhardtii can also be used as bait of aquatic organisms such as fishes and shrimps, can also be cultured in a closed culture pond, and has important application potential in the aquaculture industry.

The invention belongs to the technical field of microalgae culture, and particularly provides a haematococcus pluvialis culture medium and a preparation method thereof.The haematococcus pluvialis culture medium is prepared from 1-3 mg / L of sodium acetate, 0.01-1 mg / L of cobalt nitrate hexahydrate, 0.01-1.6 mg / L of copper sulfate, 0.1-1.2 mg / L of zinc sulfate heptahydrate, 1-3 mg / L of manganese chloride tetrahydrate, 1-10 mg / L of boric acid, 1-30 mg / L of sodium carbonate, 1100-1500 mg / L of sodium nitrate, 0.01-6 mg / L of EDTA, 20-50 mg / L of calcium chloride, 70-100 mg / L of magnesium sulfate heptahydrate, 40-100 mg / L of monopotassium phosphate trihydrate and 0.02 g of inorganic phosphorus; the method comprises the following specific steps: S1, culturing haematococcus pluvialis to a stable phase by a conventional method, inoculating haematococcus pluvialis species into a haematococcus pluvialis culture medium, and culturing; s2, shaking table; s3, culturing for 45 hours; s4, inoculating the seed solution into a haematococcus pluvialis culture medium, and continuously culturing in the shaking table for 6-15 days; the culture medium disclosed by the invention has a good promotion effect on the growth of haematococcus pluvialis and the accumulation of astaxanthin.