18 results about "Isolate - microorganism" patented technology

The disclosure relates to methods for decreasing feed conversion ratios in fowl through administering isolated microorganisms to the fowl. In particular aspects, the disclosure provides methods of decreasing feed conversion ratios in fowl through administration of a Bacillus sp. to the fowl. The disclosure further relates to isolated microorganisms and compositions comprising the same.

The invention provides a separating medium, a kit and a method for separating microorganisms in positive blood. The method for separating the microorganisms in the positive blood comprises the following steps: firstly, centrifuging a positive blood culture by adopting a vacuum blood collection tube containing separation gel, and collecting a first separation sample containing the microorganisms to be separated; and then, carrying out centrifugal treatment on the first separation sample by using different separation liquids, and carrying out collection and subsequent identification analysis on the microorganisms in the positive blood according to different densities of the microorganisms to be separated. The traditional culture technology is long in time consumption; in the prior art, microorganisms in a positive blood culture bottle cannot be rapidly and efficiently enriched and purified by pretreatment methods reported in literatures, and especially for gram-positive bacteria, the identification rates of the multiple pretreatment methods combined with mass spectrometry are low, and universality does not exist. The invention provides a rapid purification method which is simple and convenient to operate and high in identification rate and does not influence the bacterial activity, so that the inspection turnover time is shortened, and the clinical diagnosis and treatment efficiency is improved.

Disclosed are methods for isolating an analyte from a sample. In some aspects, the methods are for selectively isolating a microbial cell analyte, such as a nucleic acid, from a sample containing or suspected of containing mammalian cells. The selective isolation method includes selective lysis of the mammalian cells and filtration of the resulting lysate through a filter that retains intact microbial cells, followed by on-filter lysis of the retained microbial cells to release the microbial cell analyte. The released analyte is then eluted from the filter. In other aspects, the methods utilize on-filter lysis of a sample containing intact cells (e.g., microbial cells) to release the analytes, followed by elution of the released analytes from the filter by passing an immiscible liquid through the filter. The isolated analytes may be analyzed using a suitable assay depending on the type of analyte molecule. Also disclosed are fluidic systems and lysis solutions that may be used for isolating an analyte according to the disclosed methods.

Methods for isolating analytes from a sample are disclosed. In some embodiments, the methods are for selectively isolating microbial cell analytes, such as nucleic acids, from a sample containing or suspected of containing mammalian cells. The selective isolation method involves selective lysis of mammalian cells and filtration of the resulting lysate through a filter that retains intact microbial cells, followed by on-filter lysis of the retained microbial cells to release the microbial cell analytes. The released analytes are then eluted from the filter. In other embodiments, the methods utilize on-filter lysis of a sample containing intact cells (e.g., microbial cells) to release the analytes, followed by elution of the released analytes from the filter by passing an immiscible liquid through the filter. The isolated analytes can be analyzed using an appropriate assay depending on the type of analyte molecule.

The invention discloses a resonant ultrasonic ultraviolet sterilizer, and relates to the technical field of ultrasonic ultraviolet sterilization, the sterilizer comprises: a cavity bearing module, which is provided with a sterilization cavity, a sensing detection module, a regulation and control center module, an ultrasonic action module and an ultraviolet action module; through cooperation of a detection unit, a micro-fluidic unit and a biological sensing unit in the sensing detection module, microorganisms and suspended impurities are separated when a water body flows through a detection channel, microorganism related characteristics and turbidity related signals are accurately captured, the signals are analyzed and converted into unified electric signals through the biological sensing unit, and the unified electric signals are transmitted to the regulation and control center module; the regulation and control central module integrates the electric signal and the parameters of the microorganism characteristic database, and generates an ultrasonic parameter regulation and control instruction and an ultraviolet parameter regulation and control instruction, so that the sterilization parameters are precisely matched with the characteristics of microorganisms in the current water body, and the microorganisms are effectively killed under the synergistic effect of ultrasound and ultraviolet; the pertinence and thoroughness of the sterilization process are ensured.

The method according to the invention provides to isolate the microbial fraction from a biological sample. For this purpose, it comprises a first step of sedimentation of the non-microbial cells contained in the biological sample and a second step of sedimentation of the microbial cells contained in the biological sample.

The present application relates to agriculturally beneficial microorganisms, microbial compositions and consortia. The present disclosure relates to isolated microorganisms, novel strains including microorganisms, microbial consortia, and agricultural compositions comprising the same. In addition, the present disclosure teaches methods of utilizing the microorganisms, microbial consortia, and agricultural compositions comprising the same in methods of conferring beneficial properties to target plant species. In particular aspects, the disclosure provides methods of enhancing desired plant traits in agronomically important crop species.

The invention discloses bacillus safensis with a function of inhibiting germination of weed seeds, compost prepared from the bacillus safensis and application of the bacillus safensis. According to the invention, microorganisms are separated from soil after straw returning at the edge of the desert, and a strain NG-50 which can efficiently degrade cellulose, has an excellent nitrogen fixation function and a good stress resistance function and can resist drought stress, saline-alkali stress, acid stress and high-temperature stress is screened out; according to molecular biology identification, the strain NG-50 is bacillus safensis, and the microbial preservation number of the strain NG-50 is CGMCC (China General Microbiological Culture Collection Center) No.35363; tests prove that the strain NG-50 can effectively inhibit germination of weed seeds and remarkably promote growth of crops, can remarkably shorten the fermentation period of compost and remarkably improve the compost quality when applied to preparation of agricultural and forestry waste compost, and has the potential of serving as an agricultural and forestry waste compost decomposition agent.

The invention relates to the technical field of biomedical engineering, and discloses a method for promoting root rot resistance by analyzing soybean core endophyte flora based on fluorescence labeling, and the method comprises the following steps: S1, respectively separating microbial communities from root systems of healthy soybean plants and susceptible soybean plants to obtain a healthy group microbial suspension and a susceptible group microbial suspension; according to the method for promoting root rot resistance by analyzing soybean core endophyte flora based on fluorescence labeling, phytophthora sojae and associated microorganisms are specifically recognized through an immunofluorescence labeling technology, fluorescence-negative flora in healthy soybean root systems is precisely enriched in combination with flow cytometry sorting, screening leakage of non-culturable microorganisms is avoided from the source, and the yield of root rot resistance is improved. Meanwhile, the invalid workload in the screening process is greatly reduced, the mining period of the core beneficial endophytic flora is shortened, and the pertinence and efficiency of the screening process are improved.

A method for selectively isolating microbial cell analytes (e.g., nucleic acids) from a sample containing or suspected of containing mammalian cells is disclosed. The selective isolation method comprises selective lysis of mammalian cells, filtration of the lysate obtained by passing it through a filter that retains intact microbial cells, and subsequent release of the retained microbial cells from the filter. The microbial cells are then collected from the filter using backflow elution or aspiration, and the collected microbial cells are lysed to release the analytes from the intact cells. The isolated analytes can be analyzed using appropriate assays depending on the type of analyte molecule.

The present invention provides improved methods and kits for isolating microbial nucleic acids, preferably microbial DNA, from a tissue sample comprising a eukaryotic tissue, said method comprising the steps of: a) treating the tissue sample with a collagenase and a neutral protease that is different from the collagenase, b) treating the sample of step a) with at least one nuclease, and c) processing the sample of step b) to isolate microbial nucleic acids, preferably microbial DNA. The method significantly improves depletion of host cell DNA.

Methods and devices for isolating microbial cells from a sample, extracting eukaryotic DNA from a sample, and identifying the microbial species in the sample are disclosed herein.

A method of recovering or producing precious metals, platinum group metals, and / or rare earth elements from a starting substrate, the method comprising: contacting the starting substrate with a composition comprising an isolated microbe for an amount of time sufficient for the biological transmutation process to occur, the isolated microbe expressing a first enzyme at least 95% identical to, or at least 98% identical to, or identical to a transmutase enzyme identified by SEQ. ID. NO. 1, and a second enzyme at least 95% identical to, at least 98% identical to, or identical to an amplificase enzyme identified by SEQ. ID. NO. 2. Biological transmutation of silicon to gold. Converting phosphorus in a starting material into phosphoric acid and / or phosphonic acid. Converting heavy oil in a starting material into octane. Methods of extracting, amplifying, or producing lithium from various substrates.

The present application relates to agriculturally beneficial microorganisms, microbial compositions and consortia. The present disclosure relates to isolated microorganisms, novel strains including microorganisms, microbial consortia, and agricultural compositions comprising the same. In addition, the present disclosure teaches methods of utilizing the microorganisms, microbial consortia, and agricultural compositions comprising the same in methods of conferring beneficial properties to target plant species. In particular aspects, the disclosure provides methods of enhancing desired plant traits in agronomically important crop species.

The present disclosure relates to isolated microorganisms, including novel strains of microorganisms, microbial consortia, and agricultural compositions comprising the same. Further, the present disclosure teaches methods of utilizing the microorganisms, microbial consortia, and agricultural compositions comprising the same in methods of imparting beneficial properties to target plant species. In particular aspects, the present disclosure provides methods of enhancing desirable plant traits in agronomically important crop species.

Provided herein are methods of making microorganisms modified for increased xylose consumption as compared to unmodified microorganisms. The methods include providing xylose-consuming microorganisms comprising two or more copies of a nucleic acid sequence encoding xylose isomerase and two or more copies of a nucleic acid sequence encoding a xylose kinase, culturing the microorganisms in medium containing xylose and harvesting a portion of the microorganisms. These steps are repeated multiple times. The microorganisms are then isolated. The isolated microorganisms have increased xylose consumption rates compared to control xylose-consuming microorganisms. Also provided are a population of microorganisms made by the provided methods. Methods of culturing the population of microorganisms and methods of reducing xylitol production in cultures comprising the population of microorganisms are provided.

The utility model relates to the technical field of ostomy bags, in particular to a urinary tract ostomy bag capable of effectively isolating microorganisms, which comprises a bag body, an ostomy port arranged on the upper side of the bag body, a discharge port arranged on the lower side of the bag body, a diaphragm arranged inside the bag body and dividing the inside of the bag body into an upper cavity and a lower cavity, and a connecting pipe arranged on the lower side of the diaphragm. The upper end of the connecting pipe penetrates through the diaphragm to be communicated with the upper cavity, the peripheral side of the upper end of the connecting pipe and the diaphragm are fixed and sealed, a trap is arranged at the lower end of the connecting pipe, and a balancing weight is arranged on the trap.

Provided is a microorganism detection system by which microorganisms contained in a sample can be accurately detected even when the sample is a biological component containing ATP. This pretreatment kit for sorting microorganisms from a sample in order to measure ATP derived from the microorganisms comprises a separation unit which includes separation particles for separating the microorganisms and components other than the microorganisms, which are contained in the sample.