904results about "Microorganism lysis" patented technology

Described herein are systems and methods for analyzing biological samples. Including a method for processing an analyte, comprising providing a fluidic device comprising the analyte and one or more polymer precursors; selecting a discrete area within said fluidic device; providing an energy source in optical communication with fluidic device; and selectively supplying a unit of energy generated from the energy source to the fluidic device to generate a polymer matrix within the fluidic device, wherein the polymer matrix is within the discrete area or adjacent to the discrete area.

Described herein are systems and methods for analyzing biological samples. Including a method for processing an analyte, comprising providing a fluidic device comprising the analyte and one or more polymer precursors; selecting a discrete area within said fluidic device; providing an energy source in optical communication with fluidic device; and selectively supplying a unit of energy generated from the energy source to the fluidic device to generate a polymer matrix within the fluidic device, wherein the polymer matrix is within the discrete area or adjacent to the discrete area.

The invention relates to a preparation method of a chlorella exosome as well as a product and application of the chlorella exosome, the preparation method comprises the following steps: (1) mixing extremely micro chlorella with a separation buffer solution, and then carrying out wall breaking treatment to obtain a cell breaking solution; (2) centrifuging the cell disruption liquid, and reserving supernate; and (3) filtering the supernate to obtain the chlorella exosome. The preparation method of the chlorella exosome, provided by the invention, is simple in steps and easy to operate, the chlorella raw material and the specific separation buffer solution are subjected to wall breaking treatment, so that active ingredients in the prepared chlorella can be better extracted, the content of protein and polysaccharide in the obtained exosome is higher, and the purity of the exosome is improved. Meanwhile, gradient centrifugation, filter membrane filtration and the like are assisted in the preparation process, so that the prepared exosome is high in purity and less in impurities.

The invention relates to a universal nano biological vesicle preparation method. The preparation method comprises the following steps: carrying out crude extraction on cells from bacteria, fungi, plants or animals to obtain a crude extracting solution; the crude extracting solution is placed in a low-temperature and high-pressure homogenizer to be subjected to low-temperature and high-pressure homogenizing treatment, then the nano vesicles are obtained, the low-temperature and high-pressure homogenizer comprises a high-pressure cavity, a homogenizing valve, a working cavity, a conveying system and a temperature control system, a slit is formed in the homogenizing valve, and the crude extracting solution located in the high-pressure cavity enters the working cavity through the slit of the homogenizing valve, the pressure intensity of the high-pressure cavity is 300-3000 bar, and the working temperature of the high-pressure homogenizer is 0-10 DEG C. The activation energy is reduced by controlling high-pressure and low-temperature environments, so that the original cell membrane structure is broken, the cell wall structure is destroyed, and phospholipid bimolecules are driven to aggregate and self-assemble to form the vesicles. For biological membranes derived from bacteria, fungi, plants and animals, the vesicles can be prepared by the preparation method disclosed by the invention, and the method has universality.

The invention discloses a cordyceps militaris mycelium fermentation product as well as a preparation method and application thereof, and belongs to the technical field of daily cosmetics. A preparation method of a cordyceps militaris mycelium fermentation product comprises the following steps: taking cordyceps militaris mycelium as a strain, inoculating the strain into a liquid culture medium, and carrying out enlarged culture to obtain a seed solution; transferring the seed solution into a fermentation tank containing a liquid culture medium, introducing oxygen, stirring, and fermenting for a certain time to obtain a fermentation solution; adding an industrial compound enzyme into the fermentation liquid, and heating and stirring to obtain an enzymolysis treatment liquid; adding a certain amount of alcoholic solution into the enzymolysis treatment liquid, and fully and uniformly mixing to obtain alcohol precipitation liquid; and separating the alcohol precipitation liquid, filtering the supernatant, and collecting the clarified filtrate to obtain the cordyceps militaris mycelium fermentation product. The specific compound enzyme which is low in price and suitable for industrialization is adopted, active substances of cordyceps militaris are fully released, and the effect of the product in cosmetics is improved.

Described in several exemplary embodiments herein are serum replacement compositions, cell culture media containing said serum replacement compositions, and uses thereof.

The invention discloses a preparation method of microalgae nano-selenium and application of biotransformed nano-selenium in prevention and treatment of plant late blight. The novel preparation method of the microalgae nano-selenium comprises the following steps: firstly, extracting an intracellular extract of chlorella pyrenoidosa, and then adding sodium selenite into the intracellular extract to carry out nano-selenium conversion; compared with an existing reported method that after sodium selenite is added into a microalgae living body culture solution to be converted into nano-selenium, algae is taken to break cells, and the nano-selenium is collected, the method has the advantages that the microalgae living body does not need to be used when the nano-selenium is biologically converted, and the subsequent complicated nano-selenium separation and purification steps can be avoided; the nano-selenium obtained by converting the intracellular extract can be directly applied. Experiments prove that the effect of the nano-selenium obtained through biotransformation on inhibiting phytophthora infestans of potato late blight pathogenic bacteria is obviously better than that of chemically synthesized nano-selenium, and the nano-selenium obtained through biotransformation has an obvious effect on preventing and treating potato late blight and has an obvious effect on promoting plant growth.

The invention discloses an influenza virus maintenance fluid formula as well as a preparation method and application thereof, and particularly relates to the technical field of biological medicines. The virus maintenance liquid comprises a DMEM / F-12 basal culture medium and effective components, the effective components comprise amino acids, vitamins, salts, a virus proliferation accelerant and an additive, and the virus proliferation accelerant comprises a yeast extract. According to the invention, an influenza virus subunit ELISA detection kit is established, and an influenza virus maintenance solution formula is developed on the basis of a DMEM / F-12 basic culture medium by taking an ELISA detection result at a culture endpoint as a response value. The virus yield of the influenza virus on the MDCK-v cell matrix can be obviously improved, and the production cost is reduced.

The invention discloses a single-cell protein extraction and hydrolysis process. The invention discloses a single-cell protein extraction and hydrolysis process which comprises the following steps: S1, adding a chemical reagent into single-cell protein thalli to lyse cells to obtain a cell lysis solution; s2, adding a protein denaturation reagent into the cell lysis solution, and centrifuging to obtain a protein denaturation precipitate; s3, adding a protein dissolving reagent into the protein denaturation precipitate to obtain a protein solution; and S4, adding enzyme into the protein solution, and carrying out protein hydrolysis to obtain a hydrolysate. The method has a wide application prospect, and is expected to promote technical progress and industrial development in related fields.

The invention provides a chlorella pyrenoidosa calcium chelating peptide and a chlorella pyrenoidosa peptide calcium chelate prepared by using the chlorella pyrenoidosa calcium chelating peptide. The prepared chlorella pyrenoidosa peptide calcium chelate can enhance the maximum load and structural strength of the tibia of a mouse with osteoporosis. The chlorella pyrenoidosa calcium chelating peptide provided by the invention is combined with calcium ions to form a peptide-calcium chelate, and animal experiments prove that the peptide-calcium chelate can significantly improve the maximum load and structural strength of the tibia of a mouse with osteoporosis, and has a good effect of improving the bone biomechanical properties of the mouse with osteoporosis.

The invention provides an engineered nuclease with high salt tolerance. The polypeptide comprises one or more mutations, so that the three-dimensional structure of the polypeptide has more surface areas with positive charges. Compared with the nuclease with the SEQ ID NO: 1 sequence, the polypeptide still has at least 60% nuclease activity under the condition that the solution ion strength exceeds 200 mM.

The invention pertains to a method for reducing the viscosity of a fermentation broth, wherein the method comprises the step of mixing at least one compound selected from the group consisting of ascorbic acid, citric acid, lactic acid, formic acid, amino acids, and soluble salts of said acids into a fermentation broth comprising microbial cells, the fermentation broth having free microbial oil in an amount of 5.0 wt. % or less, calculated on the total weight of the fermentation broth, wherein the total lipid content of the dry matter content of the fermentation broth is at least 40 wt. %. The invention also pertains to a fermentation broth with a low viscosity, methods for processing the fermentation broth with a low viscosity, a dried biomass, and a use of the fermentation broth with a low viscosity in the preparation of a dried biomass, an extracted microbial oil, a feed additive, or of an animal feed.

A process for preparing a microbial cell extract for food use is described, comprising mechanically disrupting cells (eg, yeast cell), and separating the disrupted cells into fractions. The fractions can be recombined and further processed to provide texture or other qualities which resemble meat.

The invention relates to the technical field of biological agents, and particularly discloses a preparation method of a freeze-dried varicella attenuated live vaccine and the freeze-dried varicella attenuated live vaccine. The preparation method of the freeze-dried varicella attenuated live vaccine provided by the invention comprises the following steps: culturing cells, infecting the cells with viruses, and harvesting the cells. Wherein the specific steps of harvesting the cells are as follows: adding a stabilizer into the washed cells, then harvesting a cell culture by using an ultrasonic cell harvesting device, and filtering the cell culture to obtain a stock solution; the process parameters in the ultrasonic cell harvesting device are as follows: the ultrasonic power is 60-90%, the ultrasonic time is 1.0-2.5 s, and the ultrasonic frequency is 1-2; the invention also provides the freeze-dried varicella attenuated live vaccine obtained by the preparation method. The preparation method of the freeze-dried varicella attenuated live vaccine provided by the invention has the advantages of simple process steps, short preparation period, high process stability and the like, and the obtained freeze-dried varicella attenuated live vaccine is good in quality consistency and good in clinical medication safety.

The invention provides a Chinese herbal medicine composition for improving immunity as well as a preparation method and application thereof, and belongs to the technical field of medicines. The preparation method comprises the following steps: fermenting rhizoma anemarrhenae, poria cocos, rhizoma polygonati and fructus lycii to prepare Chinese herbal medicine selenium / germanium-rich polysaccharide, carrying out carboxylation modification on the Chinese herbal medicine selenium / germanium-rich polysaccharide and phycobiliprotein to prepare a Chinese herbal medicine selenium / germanium-rich polysaccharide-phycobiliprotein compound, and dissolving bacterial sludge in the fermentation process with helicase to prepare a bacteria-dissolving product; and uniformly stirring and mixing with the Chinese herbal medicine selenium / germanium-rich polysaccharide-phycobiliprotein compound, berberine and camptothecin to prepare the Chinese herbal medicine composition for improving immunity. The prepared Chinese herbal medicine composition capable of improving immunity contains rich glycoprotein and organic selenium / germanium compounds, has the effects of consolidating body resistance and strengthening yang, has the advantages of good sterilization, oxidation resistance, inflammation resistance, virus resistance and tumor resistance, no toxicity, high bioavailability, easiness in absorption and the like, is high in patient compliance and has a wide application prospect.

The invention discloses new application of Pseudomonas sp. F13, namely application of the Pseudomonas sp. F13 in splitting haematococcus pluvialis to extract astaxanthin, the Pseudomonas sp. F13 and the haematococcus pluvialis are jointly fermented and cultured, the Pseudomonas sp. F13 can split the haematococcus pluvialis, so that the astaxanthin in the haematococcus pluvialis is fully released, and the extraction rate reaches 76%. The method is simple and easy to operate, low in cost and suitable for industrial production. Solvents and wall breaking equipment are not needed in extraction, so that the negative influence on the environment is reduced, and the method is suitable for industrial production.

Different animal model tests prove that the bacteroides fragilis, especially viable bacteria, lysate, inactivated bacteria, extracts and the like of the bacteroides fragilis ZY-312 with the preservation number of CGMCC No.10685 and the bacteroides fragilis NCTC 9343 have the effects of improving and treating the functional gastrointestinal diseases, especially functional dyspepsia and irritable bowel syndrome. For functional dyspepsia, bacteroides fragilis can effectively improve weight loss, anorexia, visceral hypersensitivity and gastric motility disorder caused by functional dyspepsia. For irritable bowel syndrome, bacteroides fragilis can effectively improve diarrhea symptoms and reduce intestinal sensitivity.

The invention belongs to the technical field of biology, and relates to a viscosity reduction preparation of a cell disruption liquid and a method for reducing the viscosity of the cell disruption liquid. The viscosity reduction preparation of the cell disruption liquid comprises escherichia coli thalli for expressing nuclease of non-specific hydrolysis DNA and / or a nuclease crude product prepared from the escherichia coli thalli for expressing nuclease of non-specific hydrolysis DNA; the method for reducing the viscosity of the cell disruption liquid comprises the following steps: mixing the viscosity reduction preparation with the cell disruption liquid to form a cell disruption system, so as to reduce the viscosity of the cell disruption liquid in the cell disruption process; the method is simple, efficient and low in cost.

The invention discloses a method for purifying tetracycline and efficiently producing oil by using microalgae in a cross-temperature zone, and belongs to the field of microalgae bioengineering. The invention aims to solve the technical problem of tetracycline pollution in the current cold region water environment. The method comprises the following steps: preparing a seed solution for microalgae culture, inoculating the seed solution into a BG-11 culture medium containing tetracycline, culturing until the logarithmic growth later stage of microalgae, centrifugally collecting algae cells, freeze-drying and weighing, and extracting grease in dry algae powder by utilizing an ultrasonication combined organic solvent method. The method disclosed by the invention is simple to operate, can be used for producing biological energy and adsorbing and degrading tetracycline under the low-temperature treatment condition by utilizing the microalgae, meanwhile, the stress resistance of the microalgae under the low-temperature condition is improved, the permeability of algae cells is regulated and controlled, and the production of the microalgae biological energy is promoted. The method disclosed by the invention is used for purifying tetracycline in cross-temperature zones and efficiently producing oil.

The invention discloses a saccharomycetes lysate, a preparation method and application, and the main components of the saccharomycetes lysate comprise the following components accounting for the relative content of the saccharomycetes lysate: 93-98% of nucleoside, nucleotide and derivatives thereof; and 0.2 to 6% of amino acid; wherein the nucleoside, the nucleotide and the derivatives of the nucleoside and the nucleotide comprise one or more of adenosine, succinyladenosine, adenosine-3 '-phosphoric acid, inosine and S-adenosine homocysteine. Effect evaluation is carried out on the saccharomycetes lysate, and verification shows that the saccharomycetes lysate has the effects of whitening, repairing skin injury, preventing light and aging, resisting pollution and oxidation, resisting inflammation and relieving, protecting hair and preventing hair loss, replenishing water and preserving moisture, and enhancing skin vitality or removing dark circles.

Fluid analyzation devices, methods, and systems are disclosed including an analyzation device comprising a sample vessel having an outer surface, a microchannel within the confines of the outer surface, a first port extending through the outer surface to the microchannel, and a second port extending through the outer surface to the microchannel; and an piezo transducer bonded to the outer surface of the sample vessel to form a monolithic structure, the piezo transducer configured to emit ultrasonic acoustic waves having a first frequency, a second frequency, and a third frequency into and / or to a blood sample within the microchannel, the first frequency configured to begin separation of red blood cells and plasma in the blood sample, the second frequency configured to complete separation of the red blood cells and plasma, and the third frequency configured to rupture cell walls of the blood cells producing a lysed blood sample.

System and process are provided for fixation of carbon dioxide through fermentation. More specifically, the disclosure includes fermenting carbon dioxide into methane through methanogenic archaea and producing single cell protein nutrient supplement. The disclosure further provides the integration of methanogenic fermentation with additional processes to achieve improved carbon efficiency.

The invention discloses a genetic transformation method of PEG (Polyethylene Glycol) mediated penicillium sclerotiorum strain protoplast and application of the genetic transformation method. The method comprises the following steps: (1) culturing penicillium sclerotiorum to produce spores, germinating hyphae, and collecting and cleaning fresh penicillium sclerotiorum hyphae; (2) compounding Lysing Enzymes, Yatase, cellulase and pectinase to prepare a protoplast enzymatic hydrolysate, and then adding the protoplast enzymatic hydrolysate into the penicillium sclerotiorum hypha for enzymolysis to obtain a penicillium sclerotiorum protoplast; and (3) adding plasmids to be transformed into the penicillium sclerotiorum protoplast, and carrying out PEG (Polyethylene Glycol) mediated transformation and resistance screening to obtain a penicillium sclerotiorum protoplast transformant. According to the method, the number and regeneration rate of the protoplast can be remarkably increased, and the enzymolysis time can be effectively shortened.

The invention discloses a yeast hydrolysate as well as a preparation method and application thereof. The method comprises the following steps: preparing a saccharomyces cerevisiae HKB-145051 zymophyte solution and a geotrichum candidum zymophyte solution; inoculating the saccharomyces cerevisiae HKB-145051 zymophyte liquid to a brewer's grain fermentation medium for anaerobic fermentation to obtain saccharomyces cerevisiae zymophyte milk; inoculating the saccharomyces cerevisiae fermented bacterial milk with the geotrichum candidum fermented bacterial liquid, and carrying out aerobic fermentation to obtain bacterial milk; and adding a wall breaking agent into the bacterial milk to carry out wall breaking treatment, and then drying and crushing to obtain the yeast hydrolysate. The yeast hydrolysate provided by the invention is rich in protein content, low in flavoring substance content and relatively low in production cost, can replace part of soybean meal to be applied to livestock and poultry breeding, and has important practical significance in promoting cost reduction and efficiency improvement of the livestock and poultry breeding industry.

The present invention relates to the field of microbial technology, and discloses Rhizopus microsporus HR3-26 and its application in the extraction of boletus polysaccharide. The present invention provides a new microbial strain, Rhizopus microsporus HR3-26, and allows Rhizopus microsporus HR3-26 to grow moderately in boletus powder added with sucrose to produce various hydrolases. After that, the fermented boletus powder is heated with water, and substances such as cellulose in the cell wall are hydrolyzed, which helps the bound boletus polysaccharide to dissolve during ultrasonic water extraction, thereby significantly improving the extraction yield of polysaccharide. The present invention applies Rhizopus microsporus HR3-26 to the extraction of boletus polysaccharide. Compared with the conventional method of water extraction and alcohol precipitation, the extraction yield of polysaccharide can be increased by 41.3%.

The invention provides a post-treatment method of single-cell protein fermentation liquor and a fermentation method of single-cell protein thalli. Nuclease and cell wall lytic enzyme are respectively added into fermentation liquor containing single-cell protein thalli to treat the thalli, so that nucleic acid and cell wall residues can be reduced, and the problems that in a traditional fermentation process, the application of the thalli in food is limited due to the high nucleic acid content of the thalli, and the protein extraction efficiency is reduced due to the existence of the cell walls are solved. In addition, by optimizing fermentation conditions, the protein content of the single-cell protein is remarkably increased. The process is simple, low in cost, suitable for industrial production and capable of being widely applied to the fields of feed, food and medicine.

The invention belongs to the technical field of marine organisms, and particularly relates to a method for promoting rapid reproduction and attachment of porphyra yezoensis by using seawater bacterium culture waste liquid. Attached seawater bacteria are separated from the surface of porphyra yezoensis frond, a single colony is separated and then cultured, and a 16s rDNA sequence is cloned and identified. And after the required seawater bacteria Sulfibacterium sp. Or Tenacibacterium sp. Are obtained, the seawater bacteria are continuously cultured for 10 hours at the temperature of 28 DEG C by using a 2216E seawater bacteria liquid culture medium. And centrifuging the bacterial liquid, removing thalli in the bacterial liquid, collecting supernatant waste liquid, adding the supernatant waste liquid into a porphyra yezoensis PES culture medium in proportion, and culturing for 2-4 weeks, so that the release and attachment of a large amount of spores of the porphyra yezoensis can be remarkably promoted. According to the method, the waste liquid of seawater bacterium culture is recycled, and a new way is provided for promoting porphyra yezoensis adhesion and rapid and large-scale biomass amplification. The method can also be used for artificial construction of porphyra yezoensis algal reefs in marine ranches, achieves the purposes of rapid biomass amplification and large-scale algae attachment of the porphyra yezoensis algal reefs, and has wide application scenarios.

A method of pretreatment of a sample containing a cell for mass spectrometry, including contacting the cell with a first acidic solution containing an organic acid; and extracting a cytoplasmic component of the cell by heating the cell in contact with the first acidic solution.

A kind of high-purity yeast protein and preparation method thereof, comprising the steps of: connecting the activated yeast cell to a liquid seed culture medium, inoculating it in a fermentation medium after shaking culture, centrifugation and washing to obtain a yeast cell; configuring the yeast cell to a suspension solution, obtaining a precipitate after heat treatment; configuring the precipitate to a suspension, adding 5-50U / mL of glucanase and 5-50U / mL of cellulase at 5.0-7.0 and 30-60°C, and enzymolysis to obtain a first enzymolysis solution; adding a nonionic solubilizing agent to the first enzymolysis solution at a certain pH, and heat-treating the first enzymolysis solution at a certain temperature to obtain a second enzymolysis solution; obtaining a high-purity yeast protein after solid-liquid separation, washing and drying of the second enzymolysis solution. The preparation method of the present invention reduces the possibility of protein denaturation, and the final yeast protein product has low dietary fiber, high content of branched-chain amino acids, high protein content, and good nutritional value and processing characteristics.

The present invention relates to the field of biotechnology, and particularly to a preparation method of yeast extract rich in betaine. The present invention provides a preparation method of yeast extract rich in betaine. Among them, the yeast extract rich in betaine includes protein, amino acids, peptides, nucleotides, and betaine. The mass percentage content of protein is 50% - 75%, and the mass percentage content of betaine is 0.2% - 4%. In the present invention, yeast rich in betaine is obtained by the yeast absorbing and enriching betaine in the betaine-rich carbon source during the growth process, and then the yeast extract rich in betaine is obtained through enzymatic hydrolysis and refinement. This technology makes up for the blank of yeast extract of betaine, and is mainly involved in the fields of food, health food, feed, medicine, etc.