628results about "Microorganism lysis" patented technology

Described herein are systems and methods for analyzing biological samples. Including a method for processing an analyte, comprising providing a fluidic device comprising the analyte and one or more polymer precursors; selecting a discrete area within said fluidic device; providing an energy source in optical communication with fluidic device; and selectively supplying a unit of energy generated from the energy source to the fluidic device to generate a polymer matrix within the fluidic device, wherein the polymer matrix is within the discrete area or adjacent to the discrete area.

Described herein are systems and methods for analyzing biological samples. Including a method for processing an analyte, comprising providing a fluidic device comprising the analyte and one or more polymer precursors; selecting a discrete area within said fluidic device; providing an energy source in optical communication with fluidic device; and selectively supplying a unit of energy generated from the energy source to the fluidic device to generate a polymer matrix within the fluidic device, wherein the polymer matrix is within the discrete area or adjacent to the discrete area.

The invention provides an engineered nuclease with high salt tolerance. The polypeptide comprises one or more mutations, so that the three-dimensional structure of the polypeptide has more surface areas with positive charges. Compared with the nuclease with the SEQ ID NO: 1 sequence, the polypeptide still has at least 60% nuclease activity under the condition that the solution ion strength exceeds 200 mM.

A process for preparing a microbial cell extract for food use is described, comprising mechanically disrupting cells (eg, yeast cell), and separating the disrupted cells into fractions. The fractions can be recombined and further processed to provide texture or other qualities which resemble meat.

The invention discloses new application of Pseudomonas sp. F13, namely application of the Pseudomonas sp. F13 in splitting haematococcus pluvialis to extract astaxanthin, the Pseudomonas sp. F13 and the haematococcus pluvialis are jointly fermented and cultured, the Pseudomonas sp. F13 can split the haematococcus pluvialis, so that the astaxanthin in the haematococcus pluvialis is fully released, and the extraction rate reaches 76%. The method is simple and easy to operate, low in cost and suitable for industrial production. Solvents and wall breaking equipment are not needed in extraction, so that the negative influence on the environment is reduced, and the method is suitable for industrial production.

The invention discloses a saccharomycetes lysate, a preparation method and application, and the main components of the saccharomycetes lysate comprise the following components accounting for the relative content of the saccharomycetes lysate: 93-98% of nucleoside, nucleotide and derivatives thereof; and 0.2 to 6% of amino acid; wherein the nucleoside, the nucleotide and the derivatives of the nucleoside and the nucleotide comprise one or more of adenosine, succinyladenosine, adenosine-3 '-phosphoric acid, inosine and S-adenosine homocysteine. Effect evaluation is carried out on the saccharomycetes lysate, and verification shows that the saccharomycetes lysate has the effects of whitening, repairing skin injury, preventing light and aging, resisting pollution and oxidation, resisting inflammation and relieving, protecting hair and preventing hair loss, replenishing water and preserving moisture, and enhancing skin vitality or removing dark circles.

Fluid analyzation devices, methods, and systems are disclosed including an analyzation device comprising a sample vessel having an outer surface, a microchannel within the confines of the outer surface, a first port extending through the outer surface to the microchannel, and a second port extending through the outer surface to the microchannel; and an piezo transducer bonded to the outer surface of the sample vessel to form a monolithic structure, the piezo transducer configured to emit ultrasonic acoustic waves having a first frequency, a second frequency, and a third frequency into and / or to a blood sample within the microchannel, the first frequency configured to begin separation of red blood cells and plasma in the blood sample, the second frequency configured to complete separation of the red blood cells and plasma, and the third frequency configured to rupture cell walls of the blood cells producing a lysed blood sample.

The invention discloses a genetic transformation method of PEG (Polyethylene Glycol) mediated penicillium sclerotiorum strain protoplast and application of the genetic transformation method. The method comprises the following steps: (1) culturing penicillium sclerotiorum to produce spores, germinating hyphae, and collecting and cleaning fresh penicillium sclerotiorum hyphae; (2) compounding Lysing Enzymes, Yatase, cellulase and pectinase to prepare a protoplast enzymatic hydrolysate, and then adding the protoplast enzymatic hydrolysate into the penicillium sclerotiorum hypha for enzymolysis to obtain a penicillium sclerotiorum protoplast; and (3) adding plasmids to be transformed into the penicillium sclerotiorum protoplast, and carrying out PEG (Polyethylene Glycol) mediated transformation and resistance screening to obtain a penicillium sclerotiorum protoplast transformant. According to the method, the number and regeneration rate of the protoplast can be remarkably increased, and the enzymolysis time can be effectively shortened.

The present invention relates to the field of microbial technology, and discloses Rhizopus microsporus HR3-26 and its application in the extraction of boletus polysaccharide. The present invention provides a new microbial strain, Rhizopus microsporus HR3-26, and allows Rhizopus microsporus HR3-26 to grow moderately in boletus powder added with sucrose to produce various hydrolases. After that, the fermented boletus powder is heated with water, and substances such as cellulose in the cell wall are hydrolyzed, which helps the bound boletus polysaccharide to dissolve during ultrasonic water extraction, thereby significantly improving the extraction yield of polysaccharide. The present invention applies Rhizopus microsporus HR3-26 to the extraction of boletus polysaccharide. Compared with the conventional method of water extraction and alcohol precipitation, the extraction yield of polysaccharide can be increased by 41.3%.

The invention provides a post-treatment method of single-cell protein fermentation liquor and a fermentation method of single-cell protein thalli. Nuclease and cell wall lytic enzyme are respectively added into fermentation liquor containing single-cell protein thalli to treat the thalli, so that nucleic acid and cell wall residues can be reduced, and the problems that in a traditional fermentation process, the application of the thalli in food is limited due to the high nucleic acid content of the thalli, and the protein extraction efficiency is reduced due to the existence of the cell walls are solved. In addition, by optimizing fermentation conditions, the protein content of the single-cell protein is remarkably increased. The process is simple, low in cost, suitable for industrial production and capable of being widely applied to the fields of feed, food and medicine.

A method of pretreatment of a sample containing a cell for mass spectrometry, including contacting the cell with a first acidic solution containing an organic acid; and extracting a cytoplasmic component of the cell by heating the cell in contact with the first acidic solution.

A kind of high-purity yeast protein and preparation method thereof, comprising the steps of: connecting the activated yeast cell to a liquid seed culture medium, inoculating it in a fermentation medium after shaking culture, centrifugation and washing to obtain a yeast cell; configuring the yeast cell to a suspension solution, obtaining a precipitate after heat treatment; configuring the precipitate to a suspension, adding 5-50U / mL of glucanase and 5-50U / mL of cellulase at 5.0-7.0 and 30-60°C, and enzymolysis to obtain a first enzymolysis solution; adding a nonionic solubilizing agent to the first enzymolysis solution at a certain pH, and heat-treating the first enzymolysis solution at a certain temperature to obtain a second enzymolysis solution; obtaining a high-purity yeast protein after solid-liquid separation, washing and drying of the second enzymolysis solution. The preparation method of the present invention reduces the possibility of protein denaturation, and the final yeast protein product has low dietary fiber, high content of branched-chain amino acids, high protein content, and good nutritional value and processing characteristics.

The present invention relates to the field of biotechnology, and particularly to a preparation method of yeast extract rich in betaine. The present invention provides a preparation method of yeast extract rich in betaine. Among them, the yeast extract rich in betaine includes protein, amino acids, peptides, nucleotides, and betaine. The mass percentage content of protein is 50% - 75%, and the mass percentage content of betaine is 0.2% - 4%. In the present invention, yeast rich in betaine is obtained by the yeast absorbing and enriching betaine in the betaine-rich carbon source during the growth process, and then the yeast extract rich in betaine is obtained through enzymatic hydrolysis and refinement. This technology makes up for the blank of yeast extract of betaine, and is mainly involved in the fields of food, health food, feed, medicine, etc.

The invention relates to the field of single cell analysis of droplet microfluidics, in particular to a controllable cell lysis method in an emulsion. The invention provides a method for cell lysis in a liquid drop, which comprises the following steps: 1) liquid drop single cell wrapping: wrapping a single cell into the liquid drop through a micro-fluidic chip in the presence of a first oil phase to obtain a first mixture containing the first oil phase and the liquid drop; and 2) cell lysis: replacing the first oil phase in the first mixture with a second oil phase containing a cell lysis solution, and incubating to realize cell lysis. For realizing cell lysis in the liquid drop, the second oil phase containing the cell lysis solution can realize cell lysis of the liquid drop outside the micro-fluidic chip, so that the operation difficulty is low, the cell lysis method is simple and efficient, low-cost, high-throughput and controllable cell lysis can be realized, and the applicability is wide.

A lysis capsule for performing a cell lysis procedure includes a hollow body having an open first end and an open second end, a first porous membrane covering the open first end, and a second porous membrane covering the open second end, and the hollow body defines a lysis chamber between the first and second porous membranes. A plurality of non-magnetic beads and at least one magnetic element are disposed within the lysis chamber, and the pores of the first and second porous membranes are sized to retain the non-magnetic beads and the magnetic element within the lysis chamber. An internal control may be contained within the lysis chamber to validate an assay result and / or to validate the effectiveness of the cell lysis procedure. The lysis capsule may be disposed within a sample chamber of a fluidic cartridge.

The invention belongs to the technical field of food processing, and particularly relates to a product rich in soluble yeast dietary fibers and a preparation method and application thereof. Wherein in terms of dry matter mass of the product containing the soluble yeast dietary fiber, the product containing the soluble yeast dietary fiber comprises the following components in percentage by mass: more than 40% of soluble yeast dietary fiber, less than 0.5% of fat, more than 20% of yeast beta-glucan and more than 20% of mannan. The product containing the soluble yeast dietary fibers prepared by the preparation method disclosed by the invention contains the soluble dietary fibers with higher content, the solubility is obviously improved, the mouth feel is improved to a certain extent, and the product contains lower fat content and is more beneficial to storage; and meanwhile, the product is rich in beta-glucan and mannan, so that the product has a wide application prospect.

The invention belongs to the technical field of biology, and particularly relates to perforin derived from staphylococcus warneri mild bacteriophage and application. Perforin has a nucleotide sequence as shown in SEQ ID NO: 1. The perforin is a key gene for bacteriophage splitting growth and plays an important role in the splitting process of host bacteria. Therefore, the research aims to verify whether the perforin of which the coding site is positioned on a staphylococcus warneri mild bacteriophage genome can be used as a cell lysis gene and can be used as a potential antibacterial substance or not, and a theoretical basis is provided for prevention and treatment of gram-negative pathogenic bacteria.

The present invention discloses a preparation method of a filtrate of fermentation products of Bifidobacterium longum and its lysate, which relates to the technical field of microorganisms. A strain of Bifidobacterium longum CGMCC No. 31133 with fast growth rate, high cell density, rich and high-content metabolites is obtained through strain mutagenesis and breeding. The strain is used for fermenting to prepare a filtrate of fermentation products and a lysate. The filtrate is rich in acetic acid, lactic acid, amino acids, proteins and Bifidobacterium longum polysaccharides, and has remarkable skin brightening and moisturizing effects. The alkali lysis method is used for cell wall breaking, which not only makes the release of intracellular active substances more complete, but also effectively utilizes the active components of cell walls and cell membranes. The prepared lysate has richer active components and is easy to absorb, further enhancing the antioxidant, anti-aging, skin brightening and repair performance of the lysate. The filtrate of fermentation products and the lysate prepared by the present invention not only have rich active components and excellent performance, but also have a simple process and a short production cycle, and have significant cost advantages in production.

Described are methods for producing recombinant Arginase, such as PEGylated, cobalt-substituted recombinant human Arginase 1. Also described are pharmaceutical compositions comprising such recombinant Arginase, as well as methods of treatment and uses of such recombinant Arginase.

The invention relates to a method for extracting genome DNA of plants rich in secondary metabolites and a buffer solution, and belongs to the technical field of molecular biology and botany. The method solves the technical problems of low DNA extraction efficiency, poor purity, easy degradation and the like when a traditional DNA extraction method is used for treating plant tissues rich in secondary metabolites such as alkaloid, polyphenol and the like. Comprising the following steps: adding a complexing agent such as polyethylene glycol or polyvinylpyrrolidone when grinding plant tissues in a liquid nitrogen environment; splitting by using a cell wall splitting buffer solution containing dithiothreitol and a nonionic surfactant; carrying out DNA release and extraction at 60-70 DEG C by adopting a CTAB (Cetyltrimethyl Ammonium Bromide) extraction buffer solution containing polyethylene glycol and papain; and then purifying and precipitating to obtain high-purity genome DNA (Deoxyribose Nucleic Acid). The method can effectively remove alkaloid, protein and other impurities, significantly improves the DNA yield and purity, and is suitable for genome sequencing, genetic resource protection, medicinal plant molecular identification and the like of plants with high secondary metabolites such as Stephania kwangsiensis and the like.

The invention belongs to the technical field of microbial protein separation and extraction, and particularly relates to soluble microbial protein and a preparation method thereof. The method comprises the following steps: fermenting and culturing microbial thalli to a logarithmic phase, and centrifuging to obtain mycelia; the mycelium is freeze-dried and then smashed, and mycelium powder is obtained; the mycelium powder is placed in water to be dispersed and homogenized, and homogenized homogenate is obtained; finally, alkaline protease is added into the homogenate for enzymolysis and enzyme deactivation, and the soluble microbial protein is obtained. On one hand, microbial cell walls are directly destroyed through mechanical shearing force and collision action generated by high-speed rotation of the wall breaking machine, and intracellular proteins and active ingredients are released; on the other hand, high-pressure homogenization is combined, mechanical shear force and cavitation effect are utilized to destroy a protein compact structure and expose restriction enzyme cutting sites, and alkaline protease is combined for directional hydrolysis, so that the enzymolysis time is greatly shortened, the efficiency is improved, and the extraction rate is greatly improved.

The present disclosure relates to liquid (e.g., water-soluble) compositions comprising antimethanogenic compounds, and methods of administering the same to reduce enteric methane emissions from ruminant animals, and / or improve feed-efficiency. Certain disclosed compositions exhibit improved rapid effect over prior art compositions. The disclosure further relates to methods of concentrating antimethanogenic compounds, extracting antimethanogenic compounds, and methods of increasing the bioavailability of antimethanogenic compounds.

The invention provides a polygonatum kingianum root endophytic fungus Princasa crispa DHJ068 and application thereof, and relates to the technical field of crop disease control. The polygonatum kingianum root endophytic fungus Princephala crispa DHJ068 is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC NO: M 2025748, and a fermentation liquor crude extract of the Princephala crispa DHJ068 can be applied to prevention and treatment of crop diseases. The invention overcomes the defects in the prior art, and the fermentation liquor crude extract of the polygonatum kingianum root endophytic fungus Prringtonia crispa DHJ068 can effectively inhibit the growth of Chinese cabbage soft rot bacteria and cucumber bacterial angular leaf spot bacteria, thereby achieving the purpose of certain biological control.

The present disclosure provides an in-vitro method of universal microbial lysis compatible with hybridization-based nucleic acid enrichment and extraction methods, comprising the steps of: (a) subjecting a sample to a combination of lysis steps to obtain a lysed sample, wherein the combination of lysis steps is selected from the group consisting of: (i) an ultrasound sonication, (ii) a combination of multiple sonication steps,- (iii) a combination of enzymatic treatment and one or more sonication steps, and (iv) a combination of enzymatic treatment, one or more sonication steps, and thermal step; and (b) treating the lysed sample of step (a) with one or more enzymes, followed by sonication for microbial lysis.

Described herein are systems and methods for analyzing biological samples. The methods can include forming a plurality of compartments, wherein a compartment of the plurality of compartments may enclose a cell inside of a fluidic device. The methods can further include detecting an interaction between the cell and an additional cell within the fluidic device.

In order to control plant diseases caused by Xanthomonas bacteria, a novel bacteriophage exhibiting bacteriolytic activity specific to the same bacteria is isolated, and a plant disease control composition containing the same as an active ingredient is developed and provided. Provided are a lysozyme containing a bacteriophage having a novel genomic DNA sequence and exhibiting a lysolytic activity specific to Xanthomonas bacteria, and a plant disease control composition containing the lysozyme as an active ingredient.

The present invention is directed to a filter assembly for capturing environmental DNA (eDNA), a kit comprising the filter assembly, a method of capturing eDNA using the filter assembly, a method of analysing eDNA captured in the filter assembly, and a method of providing biodiversity data by analysing eDNA collected in the filter assembly.

When bacteria with different bacteriolysis targets are detected, it was necessary to lyse the individual bacteria separately with lysis solutions containing lysis enzymes suitable for each bacteriolysis target, resulting in a complicated and delayed bacteriolysis process. The purpose of this invention is to provide a new bacteriolysis method for lysing bacteria belonging to two or more species in a sample, making the bacteriolysis process more efficient and rapid. Provided is method for lysing a group of bacteria in the sample, comprising the step of lysing the group of bacteria in the sample in a mixture solution obtained by mixing the sample, a bacteriolysis enzyme, and a bacteriolysis aid, wherein the bacteriolysis enzyme includes at least one bacteriolysis enzyme selected from the group consisting of lysostaphin, lysozyme, acetyl glucosaminidase, and endopeptidase, and wherein the mixture solution has a pH of from 6.0 to 7.0 and an electrical conductivity of from 2.5 to 8.5 mS / cm, whereby bacteriolysis of two or more species of bacteria becomes possible.

Provided for are methods of producing triacylglycerol-accumulated microalgae, methods for making bioplastics from triacylglycerol-accumulated microalgae, methods for making alga-mixed plastics, and products including these bioplastics. Methods of triacylglycerol accumulation using centrifugation are also provided. Products such as plastic beads and other consumer products can be made from the bioplastics described herein.

The invention belongs to the technical field of microbial genetic transformation, and particularly relates to a method for high-yield production of pleurotus ostreatus transformants, a genetic transformation system and application of the genetic transformation system. Through optimization of protoplast preparation conditions, the number of the obtained protoplast is 4.95 * 10 < 8 > CFU.mL <-1 >, and the regeneration rate of the protoplast in an OM-YEPS regeneration culture medium is 12.7%. A method for producing high-yield pleurotus ostreatus transformants is researched by using a PEG-mediated protoplast transformation method, a set of efficient and stable genetic transformation and screening method is established, about 200-300 positive transformants with stably expressed exogenous genes are obtained, and the transformation efficiency is 65.38%. The genetic transformation system of the high-yield Pleurotus ostreatus transformant, constructed by the invention, lays a solid technical foundation for subsequent gene function verification, metabolic engineering transformation and molecular breeding with excellent characters, and further accelerates the biological research and industrial application process of Pleurotus ostreatus and even other macro fungi.