270results about "Microorganism lysis" patented technology

A method of pretreatment of a sample containing a cell for mass spectrometry, including contacting the cell with a first acidic solution containing an organic acid; and extracting a cytoplasmic component of the cell by heating the cell in contact with the first acidic solution.

A lysis capsule for performing a cell lysis procedure includes a hollow body having an open first end and an open second end, a first porous membrane covering the open first end, and a second porous membrane covering the open second end, and the hollow body defines a lysis chamber between the first and second porous membranes. A plurality of non-magnetic beads and at least one magnetic element are disposed within the lysis chamber, and the pores of the first and second porous membranes are sized to retain the non-magnetic beads and the magnetic element within the lysis chamber. An internal control may be contained within the lysis chamber to validate an assay result and / or to validate the effectiveness of the cell lysis procedure. The lysis capsule may be disposed within a sample chamber of a fluidic cartridge.

The invention belongs to the technical field of food processing, and particularly relates to a product rich in soluble yeast dietary fibers and a preparation method and application thereof. Wherein in terms of dry matter mass of the product containing the soluble yeast dietary fiber, the product containing the soluble yeast dietary fiber comprises the following components in percentage by mass: more than 40% of soluble yeast dietary fiber, less than 0.5% of fat, more than 20% of yeast beta-glucan and more than 20% of mannan. The product containing the soluble yeast dietary fibers prepared by the preparation method disclosed by the invention contains the soluble dietary fibers with higher content, the solubility is obviously improved, the mouth feel is improved to a certain extent, and the product contains lower fat content and is more beneficial to storage; and meanwhile, the product is rich in beta-glucan and mannan, so that the product has a wide application prospect.

The invention relates to a method for extracting genome DNA of plants rich in secondary metabolites and a buffer solution, and belongs to the technical field of molecular biology and botany. The method solves the technical problems of low DNA extraction efficiency, poor purity, easy degradation and the like when a traditional DNA extraction method is used for treating plant tissues rich in secondary metabolites such as alkaloid, polyphenol and the like. Comprising the following steps: adding a complexing agent such as polyethylene glycol or polyvinylpyrrolidone when grinding plant tissues in a liquid nitrogen environment; splitting by using a cell wall splitting buffer solution containing dithiothreitol and a nonionic surfactant; carrying out DNA release and extraction at 60-70 DEG C by adopting a CTAB (Cetyltrimethyl Ammonium Bromide) extraction buffer solution containing polyethylene glycol and papain; and then purifying and precipitating to obtain high-purity genome DNA (Deoxyribose Nucleic Acid). The method can effectively remove alkaloid, protein and other impurities, significantly improves the DNA yield and purity, and is suitable for genome sequencing, genetic resource protection, medicinal plant molecular identification and the like of plants with high secondary metabolites such as Stephania kwangsiensis and the like.

The present disclosure provides an in-vitro method of universal microbial lysis compatible with hybridization-based nucleic acid enrichment and extraction methods, comprising the steps of: (a) subjecting a sample to a combination of lysis steps to obtain a lysed sample, wherein the combination of lysis steps is selected from the group consisting of: (i) an ultrasound sonication, (ii) a combination of multiple sonication steps,- (iii) a combination of enzymatic treatment and one or more sonication steps, and (iv) a combination of enzymatic treatment, one or more sonication steps, and thermal step; and (b) treating the lysed sample of step (a) with one or more enzymes, followed by sonication for microbial lysis.

Described herein are systems and methods for analyzing biological samples. The methods can include forming a plurality of compartments, wherein a compartment of the plurality of compartments may enclose a cell inside of a fluidic device. The methods can further include detecting an interaction between the cell and an additional cell within the fluidic device.

In order to control plant diseases caused by Xanthomonas bacteria, a novel bacteriophage exhibiting bacteriolytic activity specific to the same bacteria is isolated, and a plant disease control composition containing the same as an active ingredient is developed and provided. Provided are a lysozyme containing a bacteriophage having a novel genomic DNA sequence and exhibiting a lysolytic activity specific to Xanthomonas bacteria, and a plant disease control composition containing the lysozyme as an active ingredient.

The invention belongs to the technical field of microbial genetic transformation, and particularly relates to a method for high-yield production of pleurotus ostreatus transformants, a genetic transformation system and application of the genetic transformation system. Through optimization of protoplast preparation conditions, the number of the obtained protoplast is 4.95 * 10 < 8 > CFU.mL <-1 >, and the regeneration rate of the protoplast in an OM-YEPS regeneration culture medium is 12.7%. A method for producing high-yield pleurotus ostreatus transformants is researched by using a PEG-mediated protoplast transformation method, a set of efficient and stable genetic transformation and screening method is established, about 200-300 positive transformants with stably expressed exogenous genes are obtained, and the transformation efficiency is 65.38%. The genetic transformation system of the high-yield Pleurotus ostreatus transformant, constructed by the invention, lays a solid technical foundation for subsequent gene function verification, metabolic engineering transformation and molecular breeding with excellent characters, and further accelerates the biological research and industrial application process of Pleurotus ostreatus and even other macro fungi.

A method and system for increasing the purity of protein from a system that produces protein, such as a high protein meal, from dry milling of grains including, for example, corn and wheat. The purity of the high protein meal, for example, can be increased by back end enzyme treatment of yeast, which has been separated along with protein from whole stillage. The enzyme(s) can breakdown the yeast into its yeast components that can be removed from the protein to provide for an increased purity of a resulting high protein product.

A fluid sample is dispensed into a sample chamber of a fluidic cartridge, the sample chamber containing non-magnetic beads, a magnetic element, and an internal control reagent contained within an internal control pellet including an internal control for validating an assay result and / or to validate the effectiveness of a cell lysis procedure. The magnetic element is exposed to a magnetic field, thereby causing movement of the magnetic element, which causes movement of the non-magnetic beads. Movement of the non-magnetic beads causes cells contained within the fluid sample to lyse and release nucleic acids. The internal control reagent dissolves in the presence of the fluid sample or movement of the non-magnetic beads disintegrates the internal control pellet, thereby releasing the internal control into the fluid sample. Movement of the magnetic element and the non-magnetic beads causes the internal control to be distributed within the fluid sample.

Disclosed is an electroporator device for pretreating food matrices, which comprises: a sine-wave-generating system; a remote digital activation system; an actuator; a high-voltage-generating system; a treatment chamber; a continuous food transport system; a measuring and monitoring system; and a safety and protection system. Also disclosed is a process for pretreating food matrices using the electroporator device, for long-term storage of samples having a moisture content of 2-5%. The device and process reduce dehydration method processing times and, simultaneously, allow good-quality dehydrated products to be obtained.

Disclosed are cell culture media using microorganismal, e.g., bacterial, fungal, lysates as a replacement for serum that are effective for short-term and long-term cultivation of animal cells, e.g., generation of cultivated meat. Also disclosed are systems for culturing animal cells using the media, methods of making the media, methods of detecting suitable serum replacements, and kits.

A fluidic cartridge chamber contains non-magnetic beads and a magnetic element. Motion imparted to the magnetic element by a varying magnetic field imparts motion the non-magnetic beads, thereby lysing cells of a fluid sample within the chamber. An internal control is provided within the chamber to validate an assay result and / or to validate the effectiveness of cell lysis. The internal control may be in a non-liquid form deposited on a portion of the chamber, the non-magnetic beads, and / or the magnetic element and dissolves when contacted by a fluid sample. The internal control may be provided in a pellet form that dissolves when contacted by a fluid sample or is disrupted when exposed to lysing forces of the non-magnetic beads. A fluidic cartridge includes wells that are fluidly connected, and one well comprises an expansion well to which a chamber expander is attached to expand a volumetric capacity of the expansion well.

The present invention relates to a cellular component homogenate in liquid form, as well as to a cellular component homogenate in solid form, preferably as sprayed powder. Furthermore, the present invention relates to a homogenisation process for the preparation of said cellular component homogenate in liquid form and said cellular component homogenate in solid form, preferably as sprayed powder. Lastly, the present invention relates to a composition comprising said cellular component homogenate in solid form and, optionally, one or more pharmaceutical or food grade or cosmetic additives and excipients, for use in the pharmaceutical, nutraceutical, medical devices, foods for special medical purposes, dietary supplements and food industry both in the human and veterinarian field, as well as for use in the cosmetics industry.

A high-purity yeast protein and a preparation method therefor, wherein the method comprises the following steps: inoculating an activated yeast strain into a liquid seed culture medium, culturing same in a shaker, then inoculating same into a fermentation culture medium for culturing, and centrifuging and washing same to obtain a yeast strain; preparing the yeast strain into a suspension solution, and performing a heat treatment to collect a precipitate; preparing the precipitate into a suspension, adding 5-50 U / mL of dextranase and 5-50 U / mL of cellulase at a pH of 5.0-7.0 and 30-60°C, and performing enzymatic hydrolysis to obtain a first enzymatic hydrolysate; adding a non-ionic solubilizer to the first enzymatic hydrolysate at a certain pH, and incubating the resulting mixure at a certain temperature to obtain a second enzymatic hydrolysate; and subjecting the second enzymatic hydrolysate to solid-liquid separation, washing and drying to obtain a high-purity yeast protein. The method reduces the possibility of protein denaturation. The final yeast protein product has a low dietary fiber content, a high branched-chain amino acid content and a high protein content, and exhibits good nutritional value and processing characteristics.

The application belongs to the field of cosmetics, and particularly relates to a bacillus fermentation product for repairing and moisturizing, and a preparation method and application thereof. The preparation method of the bacillus fermentation product for repairing and moisturizing comprises the following steps: S1, culturing bacillus in a culture medium to obtain bacillus spores; S2, treating the bacillus spores obtained in step S1 with lysozyme and protease, and then treating the bacillus spores with an ultrahigh-pressure homogenizer to obtain a spore lysis product; and S3, centrifuging, concentrating and heating the spore lysis product obtained in step S2 to obtain the bacillus fermentation product for repairing and moisturizing. The spore lysis product is applied to the field of cosmetic raw material preparation, and the bacillus fermentation product obtained by the application has excellent repairing and moisturizing effects, can inhibit staphylococcus aureus, and has the effect of balancing skin microecology.

The invention discloses staphylococcus warneri CCSM005 separated from human skin and capable of improving skin health and a metagen thereof, and belongs to the technical field of microorganisms and medicines. The staphylococcus warneri CCSM005 provided by the invention can be fermented to produce the L-glycine-valine dipeptide, and a fermentation supernatant of the staphylococcus warneri CCSM005 has a good effect of repairing a skin barrier function, which is specifically shown in that the activity of damaged HaCaT cells is improved in vitro, and the gene level expression of FLG, ZO-1, CLDN and Occludin can be improved at the same time after the damage. Therefore, the staphylococcus warneri CCSM005 has a huge application prospect in preparation of external daily chemical products or medicines for repairing the skin barrier.

The application provides a yeast mannose oligosaccharide and a preparation method and application thereof. The preparation method comprises the following steps: S1, mixing yeast cell walls with water to obtain a mixed solution; S2, adjusting the pH of the mixed solution to 4-9, performing first enzymolysis on the mixed solution by using a protease to form a first enzymolysis product; secondly, performing second enzymolysis on the first enzymolysis product by using a polysaccharide enzyme to obtain a second enzymolysis product; the amount of the protease is greater than or equal to 0.2% of the weight of the yeast cell walls, and the amount of the polysaccharide enzyme is greater than or equal to 0.1% of the weight of the yeast cell walls; S3, centrifuging the second enzymolysis product to obtain a supernatant and a centrifugal heavy phase; filtering the supernatant by using an ultrafiltration membrane, and the retentate is the yeast mannose oligosaccharide, the permeate is a yeast extract, and the centrifugal heavy phase is a yeast glucan. The yeast mannose oligosaccharide obtained by the application has high purity and high yield, the mannose oligosaccharide is clean, can be fermented by beneficial bacteria, and can be applied as a prebiotic in food.

The present disclosure relates, in general terms, to directed evolution, and more specifically to compositions and methods for phage-assisted directed evolution in microbes. In one embodiment, there is provided a method for directed molecular evolution, the method comprising: a) introducing a propagation-defective phage vector into a first host cell (mutagenic host) competent to propagate the phage vector, wherein the first host cell comprises a gene construct of interest (GOI) to be evolved; and wherein the phage vector allows for (i) expression of an error-prone DNA polymerase in the first host cell for mutagenesis, and (ii) replication and packaging of the GOI into infectious phage particles; b) incubating the first host cell under conditions for replication and mutagenesis of the GOI and release of phage particles comprising mutated GOIs; c) infecting a second host cell (selection host) with phage particles from step b); and d) selecting for a desirable function of the GOI in the second host cell.

The present invention relates to a treatment method for the degradation of the glycogen present in a biomass of unicellular red algae (ARU), particularly of the genus Galdieria, and to the product thus obtained.

The present invention relates to selected Cutibacterium acnes strains and / or their cell walls or postbiotics, as well as medical or nutritional uses thereof. The invention also relates to pharmaceutical or nutritional compositions containing the strains, their cell walls or postbiotics for the prevention or treatment of inflammatory diseases such as dermatitis or psoriasis or infectious diseases, particularly fungal or bacterial infections of the skin or mucous membranes.

The invention provides alkali cracking equipment and a method for extracting plasmids by using the same. The alkali cracking equipment comprises a first pipeline, a second pipeline, a third pipeline, a cell lysis reactor and a neutralization standing tank, wherein the first pipeline is used for conveying a bacterial suspension, the second pipeline is used for conveying an alkali lysis solution, the third pipeline is used for conveying an acid solution, the cell lysis reactor is used as a reaction container of alkali lysis cells, and the neutralization standing tank is used for collecting a neutralized feed liquid and carrying out standing incubation; the first pipeline and the second pipeline are intersected and then connected with the inlet end of the cell dissolving reactor; the third pipeline is intersected with the outlet end of the cell dissolving reactor and then is connected with the inlet end of the neutralization standing tank. The alkali cracking equipment can realize fixed-proportion on-line mixing and continuous flow cracking of the bacterial suspension, the alkali cracking solution and the acid solution, and the method for extracting plasmids by using the alkali cracking equipment can obtain high-quality plasmids, and is simple and efficient.

The invention discloses an edible solid-state fermentation product and a preparation method thereof. The preparation method comprises the following steps: culturing microalgae to obtain a microalgae culture, preparing a granular edible substrate, carrying out first fungus culture to obtain a fungus culture, and mixing the microalgae culture and the edible substrate to form an edible solid-state culture medium. And then, adding the fungus culture into the edible solid culture medium, and carrying out second fungus culture to form an edible solid fermentation product. And finally, performing drying treatment to form edible solid-state fermentation powder containing rich fungi and metabolites thereof, and microalgae and metabolites thereof. The edible solid fermentation product provided by the invention maintains nutritional ingredients of fungi and microalgae to the greatest extent, and cell walls of the microalgae are decomposed through the fungi, so that more microalgae metabolites are generated.

The invention relates to a method of producing extracellular vesicles from microorganism producer cells, comprising the steps of: a) placing microorganism producer cells in a liquid medium in a vessel comprising a baffle structure inside the vessel; b) rotating the vessel so as to generate extracellular vesicles from the microorganism producer cells; and c) collecting the generated extracellular vesicles; wherein the step b) of rotating the vessel comprises repeatedly changing the rotational motion of the vessel.

To provide an efficient biopolymer production method for improving the purity of biopolymers when producing biopolymers using microorganisms capable of biopolymer production. [Solution] A method for producing a biopolymer, comprising a GH25 family lysozyme treatment step in which GH25 family lysozyme is reacted with cultured cells of a microorganism capable of producing biopolymers or a treated product thereof under conditions of pH greater than 7.0.

One of the purposes of the present disclosure is to: (i) provide a novel bacteriophage having a lysolytic activity against a Salmonella bacterium such as S.Enteridis, or a lysolytic agent comprising the same; (ii) providing a bacteriophage having a broad range of hosts for Salmonella bacteria or a lysozyme comprising the same; (iii) providing a host-specific bacteriophage or an effective bacterial lysozyme of the Salmonella genus comprising the same; or (iv) providing a bacteriophage capable of effectively controlling S.Typhimurium, in particular, S.Typhimurium having a multidrug resistance, or a bacteriolytic agent containing the bacteriophage, which is capable of effectively controlling S.Typhimurium, in particular, S.Typhimurium having a multidrug resistance. The present disclosure provides a bacteriophage having a specific genomic DNA sequence, a Salmonella bacterial lysozyme comprising the same, and a composition comprising the same.

The present invention relates to a treatment method for degrading glycogens present in biomass of unicellular red algae (URA), in particular of the genus Galdiella, and to the products thus obtained.

The present application provides a kind of diandong yam root endophytic fungus fragile fruit pringlea DHJ068 and its application, it is related to crop disease prevention and treatment technical field.The diandong yam root endophytic fungus fragile fruit pringlea DHJ068 is preserved in China typical culture collection, and the preservation number is CCTCC NO:M 2025748, and the fermentation broth extract of the bacteria can be applied to crop disease prevention and treatment.The present application overcomes the shortcomings of the prior art, the fermentation broth extract of diandong yam root endophytic fungus fragile fruit pringlea DHJ068 can effectively inhibit the growth of Chinese cabbage soft rot bacteria and cucumber bacterial angular spot bacteria, and achieve certain biological control purposes.

The invention relates to the technical field of bioengineering and food engineering, in particular to a method for improving directional conversion of perigord truffle fatty acid into EPA and application of the perigord truffle fatty acid into the EPA. According to the system, a natural enzyme system and active ingredients in a nannochloropsis oculata lysate are fully utilized as biological catalytic components, and the physical strengthening effect of multi-frequency ultrasound is combined; under mild conditions, the unsaturated efficiency of fatty acid and the generation rate of EPA in a reaction system are remarkably improved. According to the method, perigord truffle is subjected to ethanol extraction, lipid components of perigord truffle are fully released, and the perigord truffle and a microalgae lysate have a synergistic effect, so that a composite reaction system with biological catalysis and physical promotion functions is constructed. The method is simple and controllable in process, has the advantages of being short in reaction time, low in energy consumption, environmentally friendly and the like, and meanwhile achieves the dual purposes of high-value utilization of perigord truffle resources and sustainable production of EPA. According to the method, an innovative solution is provided for high-value utilization of perigord truffle resources.

Microalgae Detection Method The present invention relates to a method for detecting at least one microorganism, preferably a microalga, in a sample, preferably a seawater sample, comprising the following steps: filtering the sample, extracting, purifying, and amplifying the DNA, and determining the presence and / or quantifying the microorganism to be detected in the sample. The microorganism is preferably a microalga of the genus Ostreopsis, a microalga of the species Ostreopsis cf. siamensis, or a microalga of the species Ostreopsis cf. ovata. The present invention also relates to a mixture of antisense primers suitable for the detection of a microalga of the genus Ostreopsis and / or a microalga of the species Ostreopsis cf. ovata, a kit comprising said mixture of antisense primers, and their use for the detection of at least one microalga of the genus Ostreopsis and / or a microalga of the species Ostreopsis cf. ovata. ovata.Figure for the abridged version: none.