3170results about "Biological material testing procedures" patented technology

The invention discloses convenient-to-adjust shaking-up equipment for cell culture, and relates to the technical field of cell culture. The shaking-up device comprises a shaking-up plate which is obliquely arranged in a shell, a plurality of holes are formed in the surface of the shaking-up plate, a limiting assembly is fixedly connected to the inner side face of the shaking-up plate, a driving assembly is fixedly connected to the bottom of the inner side face of the shell, and the side, close to an end cover, of the driving assembly is in spline clamping connection with the surface of the limiting assembly. The clamping assembly is fixedly connected with the inner wall of the hole of the shaking plate, the shaking plate is obliquely arranged, the culture bottle not only generates horizontal circular motion in the rotating process, but also generates up-and-down displacement along with the inclined surface of the shaking plate, and the culture bottle is subjected to a complete ascending and descending process in the process of revolving around the driving assembly for a circle; a three-dimensional composite oscillation effect is formed, so that a culture medium and a culture bottle are promoted to generate complex relative movement, the contact between cells and nutrient substances can be more efficiently promoted, and the gas exchange efficiency is enhanced.

The invention relates to the technical field of tumor biomedical engineering and organ chips, in particular to a vascularized gastric cancer organ chip and a preparation method thereof.The chip is composed of an integrated micro-fluidic main body, an annular micro-column array and an optical sealing film, and a central culture cavity is divided into a tumor area and a blood vessel area by micro-columns; the preparation method comprises the following steps: injecting a fibrous protein solution containing human umbilical vein endothelial cells and cancer-related fibroblasts into a vascular region, and adding thrombin for in-situ gelation to form a pre-vascularized network; the method comprises the following steps: mixing a patient-derived gastric cancer organ with a methacrylic acid esterified gelatin pre-polymerized solution, injecting the mixture into a tumor area, and carrying out photo-crosslinking immobilization; culturing for 7-14 days under the dynamic perfusion condition of a mixed culture medium to obtain a three-dimensional gastric cancer organ model containing a capillary network; the method is expected to be used for rapid, low-cost and high-throughput screening of chemotherapy or anti-angiogenesis drugs and accurate prediction of individual curative effects of patients.

A tool for movement sequence analysis utilizing printed circuits. The tool determines a workflow sequence based, at least in part, on one or more printed circuits within a printed organic component. The tool activates the workflow sequence utilizing applied external stimuli on the one or more printed circuits. Responsive to activating the workflow sequence, the tool analyzes a signal response from the one or more printed circuits. The tool generates one or more printing recommendations based, at least in part, on the signal response analysis.

The invention discloses a soil microbial fermentation device and a fermentation method, and relates to the field of microbial fermentation. A soil microorganism fermentation device comprises a fermentation tank and further comprises a cover plate detachably connected to the upper end of the fermentation tank; the material conveying barrel is positioned in the fermentation tank and is rotationally connected to the cover plate; the rotating sleeve is connected to the material conveying barrel in a sleeving mode, and a shifting rod is fixedly connected to the rotating sleeve; the motor drives the bevel gear for transmission, the linkage conveying barrel and the rotating shaft to rotate, and the electric telescopic rod is matched to drive the rotating sleeve to reciprocate, so that the three-dimensional stirring of the driving lever and the circulating conveying of the spiral blade are realized, a multi-dimensional stirring mode is formed, the material mixing uniformity is improved, the full contact of microorganisms and nutrient substances is promoted, and the fermentation efficiency and quality are improved; the elastic deflector rod and the counterweight ball can adaptively adjust the stirring range along with the rotating speed, eliminate stirring dead angles, accelerate the fermentation process, shorten the period, reduce the cost, and further optimize the fermentation effect and the product quality.

According to the device and the reagent for extracting the pathogen, the pathogen product separated from the uncultured sample extracted by using the device and the reagent can be subjected to a drug sensitivity test after simple culture and bacteria collection treatment, so that the culture process is shortened compared with pathogen diagnosis and identification of a clinical infected sample, and a drug sensitivity result can be obtained more quickly; and a more timely diagnosis and treatment reference is provided for clinicians.

The invention discloses an intelligent liquid supplementing and sampling system and method of a microorganism sampler and an unmanned flight system. A control module controls a sampling liquid pump to inject sampling liquid in a sampling liquid bottle into a wet-wall cyclone type sampler by a preset liquid injection amount and suspends the sampling liquid pump; the control module controls the fan to inject air into the wet-wall cyclone sampler for sampling, and adjusts the rotating speed of the fan in real time according to the flow detected by the sampling flow sensor, so that the sampling flow is maintained in a preset flow range; in the sampling process, the temperature sensor is used for detecting the temperature of the current air environment in real time per second, the humidity sensor is used for synchronously detecting the humidity of the current air environment in real time per second, and the control module is used for substituting the current temperature and the current humidity into a one-second sampling liquid evaporation capacity fitting function. And calculating the evaporation capacity of the sampling liquid in one second in the current temperature and humidity environment, controlling the working liquid supplementing time of the sampling liquid pump, and injecting the sampling liquid into the wet-wall cyclone type sampler to realize intelligent real-time liquid supplementing.

The invention relates to the technical field of cell detection, and particularly discloses a physical detection and analysis device based on stem cell characteristics, the physical detection and analysis device comprises a detection mechanism arranged in an analyzer, the detection mechanism comprises mounting plates, the mounting plates are arranged in an annular array, and the ends, close to each other, of the mounting plates are fixedly connected; according to the stem cell detection liquid preparation device, a test tube is installed in an analyzer through the detection mechanism, so that preparation of the stem cell detection liquid can be completed, and compared with a traditional stem cell detection liquid preparation process, the stem cell detection liquid preparation device has the advantages that the detection efficiency is greatly improved; the test tube needs to be opened for multiple times to extract supernate or add a buffer solution and the like, the operation is simple, the detection and analysis efficiency of the stem cells is improved, stem cell suspension and the like cannot be exposed in an external environment, the damage of the stem cells is reduced, the supernate is conveniently and accurately extracted, and the influence on subsequent detection and analysis use is avoided.

The present invention relates to a microfluidic device (1) for forming a cell aggregate comprising at least one first cell (C1) and one second cell (C2), and for individually processing selected cells of the cell aggregate: - Microfluidic channel (10); - At least one main inlet (11) for fluids containing a first cell, a second cell, and a third cell, respectively, located in the first portion (101) of the microfluidic channel; - An outlet (12) located in the second portion (102) of the microfluidic channel for controlling the flow rate of fluid within the microfluidic channel; - A first auxiliary inlet (131) for at least one first auxiliary fluid, located in the first portion (101) of the microfluidic channel upstream or downstream of the main inlet (11); - At least one cell trapping section (14) positioned between the first and second parts within the microfluidic channel; - At least one first valve to control the flow rate of the first auxiliary fluid, causing the fluid containing the first cells and the second cells to flow at a predetermined height within the microfluidic channel, thereby guiding the first cells and the second cells to the first and second capture units, respectively. Equipped with, The microfluidic device relates to a microfluidic device in which each cell capture section (14) comprises at least one first capture section (141) and one second capture section (142), each first and second capture section being sized to accommodate a first or second cell, the first and second capture sections being adjacent to each other in a direction perpendicular to the bottom (100) of the microfluidic channel, and forming a cell aggregate containing the captured first and second cells, each cell being at a different height relative to the bottom of the microfluidic channel.

Provided are a method and system for enriching natural killer (NK) cells in a blood sample.

The invention discloses an air microorganism aerosol alarm sampling system and method and an unmanned aerial vehicle system. A main controller receives a current position positioned by a GPS positioning module, and when the current position is in a to-be-detected area, an air pump and an alarm sampling optical module are started to pump air into the alarm sampling optical module; the alarm sampling optical module samples microbial particles in the air to form a fluorescence signal, the main controller analyzes the aerosol concentration according to the fluorescence signal and identifies whether the aerosol is a biological aerosol, when the aerosol concentration exceeds a set threshold value or the aerosol is identified as the biological aerosol, the alarm is controlled, and meanwhile, the sampling module is controlled to be started; the main controller controls the liquid supplementing peristaltic pump to start, the sampling liquid in the sampling liquid bottle is pumped into the sampler, then the motor driver is controlled to drive the fan to start to enable air to enter the sampler for sampling, only the sampling peristaltic pump is controlled to start after sampling is completed, and the sample liquid in the sampler is conveyed into the sample bottle through the three-way pipe. The collection of the microbial aerosol particles is completed.

The invention relates to the technical field of egg inoculation, in particular to an egg inoculation machine capable of achieving safe inoculation and an operation method.The egg inoculation machine comprises a conveying unit, an optical detection unit, a mechanical arm assembly and an inoculation assembly; the conveying unit is used for conveying eggs, the position of an air chamber is recognized through the optical detection unit, an injection point is determined, the inoculation assembly is installed on the mechanical arm assembly, and the mechanical arm assembly drives the inoculation assembly to conduct inoculation along the injection point; according to the egg inoculation machine, after punching is completed, the injection needle tube is directly inserted into an egg along a punched hole for safe inoculation, the requirement for visual positioning of the hole can be reduced, a mechanical arm assembly low in precision can be used, and the device cost is reduced.

The invention provides a sampler and a sampling method for collecting biological molecules on the surface of tissue, the sampler is provided with a sampling direction, the sampler comprises a main body, the main body is provided with an arc-shaped track, the center of the arc-shaped track corresponds to the center of the main body, and the sampling direction of the main body is equal to that of the arc-shaped track in one length direction of the track. And the radius of the arc-shaped track is gradually increased or decreased. According to the sampler for collecting the biological molecules on the surface of the tissue, a plurality of sampling needles are arranged, a sampling liquid drop corresponding to a sampling center can be formed and maintained among the output ends of the plurality of sampling needles, and a non-embedded sampling mode is realized through contact between the liquid drop and the surface of the biological tissue; the damage to the integrity of key biomolecules possibly caused by a traditional method is avoided, the acquisition of high-fidelity biomolecules is facilitated, and the sampler can reduce physical disturbance and mechanical damage to fragile tissues to the maximum extent.

Disclosed herein are systems and devices for wound therapy and related methods of use. The systems and devices for wound therapy and related methods of use disclosed herein may comprise one or more cartridges for preparation of a regenerative epidermal suspension. One or more cartridges for preparation of a regenerative epidermal suspension may comprise a cover with a raised processing opening, a cup that can be received within the raised processing opening and has a screen, and a well plate that is positioned under the cover, has a well, and rotates relative to the cover such that the well aligns with the raised processing opening. One or more cartridges for preparation of a regenerative epidermal suspension comprises a cover with an opening that can receive a tissue sample.

The invention discloses a coarse feed full-automatic fermentation device capable of self-adapting to multiple materials and a use method thereof, and relates to the technical field of feed fermention.The coarse feed full-automatic fermentation device comprises a tank body, an upper cover is fixedly connected to the upper end of the tank body, a discharging port is formed in the center of the bottom of the tank body, a first motor is installed at the top end of the upper cover, and multiple sets of feeding ports are evenly formed in the outer wall of the upper cover; a feeding control module, a transmission assembly and a feeding opening blocking plate are arranged in each feeding opening, and the output end of the first motor is connected with the stirring assembly and the auxiliary discharging module through a driving shaft; through the arrangement of the feeding control module and the stirring assembly, the problem of non-uniform mixing caused by different proportions of raw materials can be solved, and meanwhile, the discharging speed of fermented feed can be increased through the structural change of the auxiliary discharging module during discharging.

The invention discloses a portable field screening device for environmental remediation microbial strains, and relates to the field of environmental remediation. The invention relates to a portable field screening device for environmental remediation microbial strains, which comprises a tank body which is a high-strength and light-weight aluminum alloy tank, and further comprises a filter cartridge arranged in the tank body, a filter element arranged in the tank body, and a filter element arranged in the tank body, the driving motor is fixedly connected to the upper end of the tank body; the driving shaft is fixedly connected to the output end of the driving motor; solid-liquid separation of the soil turbid liquid is efficiently completed through a centrifugal and ultrafiltration graded filtration mode, compared with a traditional method, the device has the advantages of being high in portability, high in separation efficiency and good in effect, impurities can be accurately intercepted, microbial losses are reduced, microbial strains with specific functions are rapidly screened for an environment restoration site, a high-quality bacterium-containing liquid sample is provided, and the method is suitable for popularization and application. The efficiency and accuracy of screening of microbial strains in environment restoration work are improved, and efficient development of the environment restoration work is promoted.

The invention discloses a microbial fermentation pretreatment system based on straw treatment, and particularly relates to the technical field of microbial diagnosis degradation, the microbial fermentation pretreatment system comprises a straw recovery pretreatment module used for straw recovery to obtain pretreated straw; the fermentation reaction module is used for performing microbial strain inoculation on the pretreated straws; the multi-parameter detection module is used for measuring characteristic parameters of the fermentation process in the tank in real time; the parameter processing optimization module is used for calculating fermentation efficiency scores of the characteristic parameters and generating pre-processing parameter optimization values; and the closed-loop control module is used for carrying out pretreatment regulation and control according to the parameter optimization value. The microbial fermentation pretreatment system based on straw treatment is constructed through the straw recovery pretreatment module, the fermentation reaction module, the multi-parameter detection module, the parameter treatment optimization module and the closed-loop control module, and the problems that fermentation parameter regulation is lagged, the degradation conversion efficiency is insufficient, the fermentation process is unstable and the operation cost is high are solved.

Modular mucosal tissue bioreactors combined with bioartificial mucus provide a high-fidelity, medium-throughput platform for evaluating CF treatments and modeling host-pathogen biology, a stepping stone towards better options for HEMT-nonresponsive patients. Whole lung ex vivo Cystic Fibrosis model system allows for investigation of candidate CF therapies.

The present invention provides a long-period high-pressure enrichment culture and online monitoring apparatus and method for deep-sea microorganisms. The apparatus can simulate extreme deep-sea environments such as high pressure, low temperature, and oligotrophic conditions. Through pre-culture and graded serial culture, stable long-period growth and reproduction of the deep-sea microorganisms are ensured. An online system is used for monitoring the abundance, activity, and culture conditions of the microorganisms in real time to determine an appropriate timing for strain subculturing, and timely interactive subculturing and culture are performed, thereby achieving stable supply of high-abundance, high-activity microorganism enrichment culture solution. The present invention achieves precise pressure-retaining abundance counting of the microorganisms under in-situ pressure conditions and accurate control of a microbial growth cycle during enrichment culture, effectively improving timeliness and culture efficiency of the microorganism enrichment culture solution, and enabling long-period acquisition of high-abundance, high-activity microbial strain liquid.

The invention discloses a modified microfiltration membrane for enriching biological particles and a preparation method and application thereof, and belongs to the technical field of biological separation.The preparation method of the modified microfiltration membrane comprises the following steps that a mixed cellulose ester microfiltration membrane is provided and subjected to alkali liquor activating treatment; placing the activated membrane in a weakly alkaline buffer solution for balancing; the preparation method comprises the following steps: in a weakly alkaline buffer solution, firstly contacting the surface of the membrane with a tannic acid solution to carry out a first reaction, and then directly adding a polyethyleneimine solution to carry out a second reaction under the condition of not replacing a system, so as to form a phenol-amine cross-linked network on the surface of the membrane in situ; sodium cyanoborohydride is continuously added for reduction stabilization treatment, and the modified microporous filter membrane is obtained. The modified microporous filter membrane prepared by modifying the microporous filter membrane with tannic acid and polyethyleneimine can realize one-step synchronous enrichment of all negatively charged targets, and the problems of target loss, efficiency loss and the like caused by a multi-step process are avoided from the source.

The invention discloses anhydrous alcohol production fermentation equipment, and relates to the technical field of anhydrous alcohol production, the anhydrous alcohol production fermentation equipment comprises an installation support, a fermentation tank body is fixedly installed on the installation support, the bottom end of the fermentation tank body is fixedly communicated with a blanking pipe, the fermentation equipment also comprises a fermentation tank cover, a fermentation tank cover and a fermentation tank, the top end of the fermentation tank cover is mounted at the top end of the fermentation tank body, the top of the fermentation tank cover is fixedly communicated with a feeding pipe, and a sealing cover is mounted at the top end of the feeding pipe; the stirring assembly comprises a driving motor, a spiral material conveying rod, a fixing plate, two stirring rods, two transmission gears, a transmission gear ring and two cleaning scraping plates, and the driving motor is fixedly installed at the top of the fermentation tank cover. The interior of the fermentation tank body can be conveniently cleaned, the operation is convenient and fast, the cleaning convenience and effect are improved, subsequent use is facilitated, and the practicability is high.

An integrated modular microphysiological system is provided which includes a two or more chambers and a vascular network which includes at least one channel. The chamber can be configured for culturing a tissue and includes a layer of endothelial cells which forms an endothelial barrier within the well. The endothelial barrier can be in fluid contact with at least one of the at least one channels in the vascular network. The endothelial barrier can also be in fluid contact with a fluid in the chamber.

A pipette tip extension having a proximal end, a distal end, and an exterior wall extending between the proximal end and the distal end is disclosed. The exterior wall has an outer side and an inner side and encloses an inner cavity which is delimited by the inner side of the exterior wall. The exterior wall forms at the proximal end a reception aperture. The pipette tip extension further has one or more distance elements arranged at the inner side of the exterior wall and protruding into the inner cavity.

The present disclosure provides bioreactors and methods of producing the same. Bioreactors of the present disclosure may include a macrostructure comprising a plurality of fluidically connected minimodules that form a plurality of channels. A channel may comprise a gas permeable layer configured to provide gas diffusion to or from the channel. The macrostructure may be manufactured using three-dimensional printing of a resin. The resin may comprise a polymerizable component, photoinitiator, thermocuring agent, or any combination thereof. One or more surfaces of the microfluidic macrostructure may comprise a biocompatible coating.

The invention discloses a method suitable for extracting extracellular small vesicles in fresh water pearl shell tissue waste liquid, which comprises the following operation steps: (1) solid-liquid separation: slaughtering pearl shells, and filling slaughtering waste liquid into a waste liquid crude extraction device; (2) waste liquid crude extraction: performing multi-stage gradient filtration and gravity settling on the slaughtering waste liquid by using a waste liquid crude extraction device to obtain clarified waste liquid; (3) removing impurities: removing impurities for the first time and removing impurities for the second time; (4) concentration of the extracellular vesicles: concentrating the extracellular vesicles by using an ultra-speed centrifuge to obtain a high-concentration extracellular vesicle solution; and (5) preservation of extracellular vesicles: according to the final volume of the high-concentration extracellular vesicle solution obtained in the step (4), carrying out sample standardization through protein concentration, sub-packaging the sample, and carrying out long-term preservation in a low-temperature environment. The particle sizes of the extracted vesicles are concentrated, high-purity and complete extracellular small vesicles can be obtained, and the method is suitable for biomedical research and product development.

A method includes introducing a suspension including cells suspended in a cell culture medium through a feed port or a drain port into a cavity of a cell culture vessel, the suspension being in an amount sufficient to cover a gas permeable, liquid impermeable membrane positioned at a bottom of the cell culture vessel, the feed port being disposed through a surface of the cell culture vessel and configured to permit additional cell culture medium into the cavity, and the drain port being disposed through the surface of the cell culture vessel and configured to permit removal of the cells, cell culture medium, and used cell culture medium from the cavity, allowing the cells to settle on the gas permeable, liquid impermeable membrane by gravity, removing the used cell culture medium through the drain port and introducing the additional cell culture medium through the feed port such that a constant volume is maintained in the cell culture vessel until the cells expand to a desired cell density, wherein the removing and introducing are performed subsequent to allowing the cells to settle on the gas permeable, liquid impermeable membrane, resuspending the cells in the cell culture medium in the cell culture vessel, wherein the resuspending is performed after the desired cell density is attained, and removing the resuspended cells and the cell culture medium through the drain port.

The utility model provides a bioreactor device for replacing the function of a renal tubule, the bioreactor device is placed in a cell culture incubator, the bioreactor device comprises a fixed frame and a chip, the chip is arranged on the fixed frame, and a vein-imitated renal tubule structure is formed in the chip; openings in the two ends of the flow channel are respectively a liquid inlet and a liquid outlet; the inner diameter of the main flow channel is larger than that of the micro flow channel; wherein the inner diameter of the main flow channel and the inner diameter of the micro flow channel of the chip are both within the pipe diameter range of the physiological renal tubule; a human renal tubular epithelial cell suspension enters the vein-imitating flow channel from the liquid inlet and is attached to the inner wall of the vein-imitating flow channel. Therefore, the structure of the renal tubule is reflected in a more bionic and more reasonable manner, the physiological environment of the renal tubule can be simulated more accurately, a new design thought and method are provided for a renal tubule bioreactor device in a bioartificial kidney, the difficulty in the field of kidney replacement therapy is expected to be solved, and the life quality of patients with end-stage renal failure due to nephropathy is improved.

The invention discloses a bioreactor multi-parameter adaptive control system and method based on machine learning, and belongs to the technical field of bioreactor intelligent control, and the bioreactor multi-parameter adaptive control system comprises a bioreactor hepatocyte culture unit, a data acquisition and control unit and a machine learning intelligent decision-making unit. And the data acquisition and control lower computer controls operation variables such as gas supply, sampling and perfusion in the culture unit execution mechanism, collects culture process data returned by the parameter detection module, constructs a data-driven cell state prediction model by utilizing machine learning, and optimizes model parameters and operation variables based on a prediction result to realize closed-loop control. The method can overcome the defect that there is no precise mechanism model for hepatic cell culture, and multi-parameter collaborative optimization can be realized only depending on process data, so that the state of the hepatic cells is sensed in real time, full-cycle optimization of cell viability and functions is realized, and the activity and density of hepatic cell culture are effectively improved.

Systems and methods of clarifying a liquid suspension at high speeds contained in a flexible bag is provided. The method includes nesting at least one flexible bag containing the liquid suspension within at least one bag adapter with the at least one inlet line and / or outlet line extending from the flexible bag to an exterior of the bag adapter. The method further includes at least partially enclosing the at least one inlet line and / or outlet line within a crown cavity defined by a crown assembly. The method further includes centrifuging the at least one bag adapter and the at least one flexible bag containing the liquid suspension in a centrifuge to produce a super-natant fluid and a pellet in the at least one flexible bag.