6258results about "Biological material testing procedures" patented technology

Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.

Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.

Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.

The invention discloses an intelligent pretreatment workstation for microbiological detection, and relates to the technical field of microbiological detection. The module comprises a culture dish turntable, a transmission part, a homogeneous slapping part, a vortex oscillation part, a material rack storage part, a culture medium feeding part and the like; the culture dish turntable module comprises an upper dish station, a liquid injection station, an inoculation / coating station, a lower dish station and the like, as well as a transplanting electric module and a shaking-up module; the liquid injection unit can be used for injecting three culture solutions; the culture dish conveying module is responsible for conveying culture dishes to corresponding stations and is connected with an external feeding and receiving unit; the material rack storage module is used for storing pipettor tips, test tubes and homogenizing bags; the homogeneous slapping and vortex oscillation module is used for sample dilution and homogenization; the clamping robot is responsible for conveying the homogeneous bag and the test tube to the corresponding module and pulling out the plug of the test tube; the pipetting robot is used for sucking a sample and filling the sample into the culture dish. The steps from sample to culture can be completed at high speed and high efficiency, and the steps can be adjusted according to actual detection conditions through modular combination.

The present disclosure provides systems and methods for processing cells. The systems of the present disclosure may include a compartment, a three-dimensional (3D) matrix, a fluid flow path, an analysis unit, a preservation unit, or any combination thereof. The methods of the present disclosure may process cells via, for example, expansion, culturing, gene delivery, activation, or any combination thereof. The systems and methods described herein may be useful for producing cell products including, for example, cell therapy products.

According to the online enrichment and automatic sorting device and method for deep-sea microorganisms in the high-pressure environment, multi-stage enrichment is carried out by simulating the high-pressure and special-temperature environment of deep sea, and meanwhile, based on environment index changes observed online in the enrichment device, the deep-sea microorganisms in the high-pressure environment can be automatically sorted; the optimal time point of microbial passage is automatically judged, and a culture is transferred to a next-stage enrichment device, so that the problems that in a high-pressure environment, microbial enrichment is difficult to transfer at the optimal time point, manual operation is complicated and the like are effectively solved, and the early-stage enrichment efficiency of obtaining a single species of deep-sea microorganisms is improved; besides, automatic streak separation and automatic secondary sorting of the enriched substances are carried out in a culture medium in a high-pressure environment, and specific single colonies are automatically transferred to a pure culture kettle, so that the problems of overlapping of single colonies, low separation degree, complicated manual operation, difficulty in automatic secondary sorting in the high-pressure environment and the like in an existing high-pressure sorting technology are solved; and the high-pressure environment deep-sea microorganism sorting efficiency is greatly improved.

The invention provides a wheat stripe rust early warning method, system and device, a storage medium and a program product, and relates to the technical field of agriculture, and the method comprises the steps: obtaining the spore capture amount, meteorological data and field morbidity of a to-be-monitored farmland; the to-be-monitored farmland is used for planting to-be-monitored wheat, the spore capture amount is the capture number of puccinia striiformis wheat specialized spores, and the field morbidity is the probability that the to-be-monitored wheat is infected with wheat stripe rust; based on the meteorological data, the spore capture amount and the field morbidity, constructing a wheat stripe rust prediction model; and based on the wheat stripe rust prediction model, performing wheat stripe rust monitoring and early warning on the to-be-monitored farmland. By means of the mode, efficient, accurate and real-time monitoring and early warning can be conducted on the wheat stripe rust without depending on manual investigation, the accuracy rate is high, the efficiency is high, and outbreak of the wheat stripe rust can be effectively prevented.

The invention discloses a maltodextrin waste residue treatment device and method.The maltodextrin waste residue treatment device comprises a fermentation tank, a stirring shaft, a stirring paddle, a first driving mechanism and an air guide bin, the interior of the stirring shaft is hollow to form a first air channel, the interior of the stirring paddle is hollow to form a second air channel, the first air channel is communicated with the second air channel, and a plurality of aeration holes are formed in the stirring paddle; a connecting rod, a spring and a plurality of blocking blocks provided with air passing holes are arranged in the second air channel, the blocking blocks are connected in series through the connecting rod, the spring pulls the connecting rod to block the aeration holes through the blocking blocks, a pull rope is arranged in the first air channel, one end of the pull rope is fixedly connected with the connecting rod, the pull rope pulls the connecting rod to move horizontally, and the air guide bin is installed at the top of the stirring shaft. The air guide bin is rotationally connected with the stirring shaft, an air inlet pipe is arranged on the air guide bin, and an air inlet hole is formed in the stirring shaft. According to the method, the maltodextrin waste residues are subjected to pulse feed fermentation, the ineffective oxygen supply proportion is reduced, the problem of waste of a large amount of energy is solved, the environment is protected, and enterprise benefits are improved.

A reinforcing and scaling construction for a bioprinted tissue model, which comprises a casing and sealing bioink, the casing comprising an inner module comprising a casing base (1) and a perforated cover (2), and an outer module comprising a container (3) comprising technological valves (5), an outer cover (4) and plugs (6) for technological valves (5). The invention also relates to a method for assembling the reinforcing and sealing construction.

The invention belongs to the technical field of inspection and extraction, and particularly relates to an automatic T lymphocyte suction device which comprises an automatic suction device body, the automatic suction device body comprises a bearing table, a rotating disc with a circumferential rotating function is arranged on the bearing table, and a plurality of centrifugal assemblies are evenly distributed on the rotating disc. A centrifugal tube feeding assembly facilitating feeding of centrifugal tubes is arranged in front of one side of the bearing table, a tube carrying and conveying assembly is arranged on the portion, located on one side of the centrifugal tube feeding assembly, of the bearing table, a tube feeding mechanism is arranged at the end of the conveying belt mechanism, and a reaction reagent adding assembly is arranged on one side of the tube carrying and conveying assembly. A sample feeding assembly is arranged on one side of the upper portion of the workbench, and a first suction assembly is arranged above the multi-direction adjusting assembly. According to the invention, multiple samples can be processed in batches, the working efficiency and the cell extraction purity are improved, the influence of manual operation on the result is reduced, the accuracy of the detection effect is ensured, and the use requirements are met.

The invention discloses a probiotic fermentation device, and relates to the technical field of fermentation devices, the probiotic fermentation device comprises a fermentation tank, a tank cover is mounted on the fermentation tank, a fixed plate is fixedly connected to the inner wall of the fermentation tank, a gas collection plate is slidably connected to the inner wall of the fermentation tank close to the upper part of the fixed plate, and a ventilation shaft is rotatably connected to the middle part of the tank cover; the middle part of the gas collecting plate is slidably connected to the outer surface of the ventilation shaft, and the outer surface of the middle part of the ventilation shaft is rotatably connected to the fixed plate; compared with the prior art, the device has the advantages that the ventilation shaft rotates under the action of driving force, the ventilation stirring plate can be driven to move together, comprehensive stirring operation is carried out on mixed thalli in the fermentation container, uniform distribution of the thalli is promoted in the stirring process, the contact opportunity of the thalli and oxygen is greatly increased, and the fermentation efficiency is improved. Therefore, the fermentation rate and efficiency of the probiotics are obviously improved.

The invention discloses convenient-to-adjust shaking-up equipment for cell culture, and relates to the technical field of cell culture. The shaking-up device comprises a shaking-up plate which is obliquely arranged in a shell, a plurality of holes are formed in the surface of the shaking-up plate, a limiting assembly is fixedly connected to the inner side face of the shaking-up plate, a driving assembly is fixedly connected to the bottom of the inner side face of the shell, and the side, close to an end cover, of the driving assembly is in spline clamping connection with the surface of the limiting assembly. The clamping assembly is fixedly connected with the inner wall of the hole of the shaking plate, the shaking plate is obliquely arranged, the culture bottle not only generates horizontal circular motion in the rotating process, but also generates up-and-down displacement along with the inclined surface of the shaking plate, and the culture bottle is subjected to a complete ascending and descending process in the process of revolving around the driving assembly for a circle; a three-dimensional composite oscillation effect is formed, so that a culture medium and a culture bottle are promoted to generate complex relative movement, the contact between cells and nutrient substances can be more efficiently promoted, and the gas exchange efficiency is enhanced.

The invention discloses tissue cell activity detection equipment, and relates to the technical field of cell activity detection.The tissue cell activity detection equipment comprises a detection box, a box door is hinged to the outer wall of one side of the detection box, and a culture plate is slidably connected to the bottom of an inner cavity of the detection box; at least four sliding grooves are symmetrically formed in the inner wall of the detection box, a detection person pulls an operation handle to promote a connecting base to slide along the surface of a limiting column, a gear is meshed with a plurality of racks to promote the gear to drive a liquid storage tank to rotate by 180 degrees in a reciprocating mode, and then cell suspension is continuously shunted through a plurality of stop levers. Therefore, the liquid storage tank can be continuously rotated when the cell suspension is dripped, so that the cell suspension can be prevented from being in a static state for a long time to generate a precipitation phenomenon, the cell density distribution of the cell suspension in the holes in the culture plate can be ensured to be uniform, the accuracy and the reliability of a detection result can be improved, and the detection efficiency is improved. The quality of detection data can be improved, and meanwhile, the time and the cost of repeated detection due to inaccurate detection results are reduced.

The invention belongs to the technical field of bacterial culture, and discloses a high-throughput automatic monoclonal screening and picking workstation, which comprises a protective box for protection and isolation, and a workbench is arranged in the protective box; the plate stack module comprises a rotating plate stack arranged at the bottom end of the workbench, and a plurality of columns of culture dishes are arranged on the rotating plate stack in a lifting manner; the transfer module is arranged on the workbench and used for transferring culture dishes; the pipetting module comprises a pipetting assembly and a material storage assembly, and the pipetting assembly is used for transferring strains in the culture dish into the material storage assembly; the identification module is used for identifying and positioning the strains in the culture dish and then controlling the pipetting assembly to accurately transfer the strains. The device is simple in structure and flexible and convenient to use, high-throughput screening and picking of strains can be fully automatically carried out in a clean closed environment, manual intervention is not needed, the screening efficiency is high, strain pollution caused by manual intervention is avoided, the screening precision is high, the screening cost is reduced, and experiments are greatly facilitated.

The invention relates to a multi-field coupling model test monitoring device and method for evaluating microbial mineralization homogeneity. The device comprises a test model box, a pressurization system, a grouting system, a sampling system, a sensor assembly and a computer monitoring system. Compared with the prior art, the device is focused on researching the process of influence of different overlying stresses and grouting rates on microorganism mineralized and solidified soil in multiple environments of a biological field, a chemical field, a stress field, a hydraulic field and the like. The device can effectively evaluate the uniformity and effect of solidification and restoration of microorganism mineralized and solidified soil and the distribution condition of mineral content, and provides an important basis for researching the long-term safety of the microorganism mineralized and solidified soil. The scheme is of great significance to remediation and utilization of polluted soil and energy conservation and carbon reduction of engineering application.

The invention discloses a bacteriophage reagent production filtering device facilitating impurity cleaning, and relates to the technical field of filtering devices.The bacteriophage reagent production filtering device comprises an impurity cleaning mechanism and a collecting frame installed at the bottom of the impurity cleaning mechanism, a mixing mechanism is arranged at the top of the impurity cleaning mechanism, and a feeding pipe is arranged at the top of the mixing mechanism; the impurity cleaning mechanism comprises a filter box, a centrifugal motor is fixedly installed on one side of the middle of the filter box, the output end of the centrifugal motor is fixedly connected with a rotating block, a first rotating support is fixedly installed on one side of the rotating block, and rotating discs are symmetrically installed at the two ends of the first rotating support; a plurality of connecting sliding grooves are formed in the rotating discs, a plurality of first filter screen plates are connected between the two rotating discs in a sliding mode, full filtration of a phage reagent can be achieved by arranging the multiple sets of first filter screen plates, and impurities subjected to preliminary filtration can be filtered for the second time through the centrifugal effect of the multiple sets of first filter screen plates.

The invention relates to the technical field of deep-sea microorganism nucleic acid detection, in particular to a long-term experimental platform for deep-sea microorganism in-situ nucleic acid detection and a detection method. According to the invention, a water sample is subjected to tangential flow filtration and concentration through the filtration and sampling module, so that a concentrated microorganism sample is obtained. The method comprises the following steps of: transferring a sample into an amplification detection module under reduced pressure, transferring into a lysis purification module for cell lysis, injecting the cracked sample into a chromatographic column, filtering impurities and intercepting DNA by the chromatographic column, then washing by using an eluent to obtain a high-purity DNA sample, loading a suction head through a transfer washing liquid gun, mixing the high-purity DNA sample with an enzyme solution, adding into the amplification detection module for reaction, and detecting the DNA in the amplification detection module. And carrying out fluorescence labeling on the amplified gene, obtaining an amplification curve by using a fluorescence detector, and detecting the in-situ nucleic acid of the deep-sea microorganisms according to the change of the amplification curve. An automatic cleaning assembly is designed in the filtering and sampling module, and the cracking and purifying module is used for sampling and cleaning a cracking chamber and switching pipelines, so that cross contamination caused by deep sea in-situ multiple detection is prevented.

To provide a waste liquid withdrawing system that can smoothly withdraw waste liquid without scattering the liquid, and a cell culture system.SOLUTION: A waste liquid withdrawing system comprises: a waste liquid withdrawing mechanism which withdraws culture solution not in use anymore from a plurality of flasks arrayed in a horizontal direction as waste liquid; and a recovery mechanism having a recovery container which recovers waste liquid withdrawn by the waste liquid withdrawing mechanism, where the waste liquid withdrawing mechanism comprises: a sucking mechanism which has a suction part that sucks waste liquid in the flasks, and leads waste liquid to the recovery mechanism; a support mechanism having an arm to which suction part is attached, which is provided movably in a vertical direction, and rotates about an axis line extending in a vertical direction to turn the suction part; a cleaning part which is arranged on a trajectory of the suction part viewed from a vertical direction, and reserves cleaning fluid; and a moving mechanism which linearly moves the plurality of flasks in a horizontal direction, and arranges one flask on a trajectory viewed from a vertical direction.SELECTED DRAWING: Figure 3

The invention discloses a fermentation device for traditional Chinese medicine decoction piece production, and belongs to the field of traditional Chinese medicine fermentation. A fermentation device for producing traditional Chinese medicine decoction pieces comprises a fermentation tank and further comprises a controller fixedly arranged at the bottom of the fermentation tank; the sealing cover is in threaded connection with the top of the fermentation tank; the rotating shaft is rotationally arranged on the sealing cover; the motor is fixedly arranged at the top of the fermentation tank, and the output end of the motor is fixedly connected with the rotating shaft; the hemispherical head is fixedly arranged at the bottom of the rotating shaft; the limiting groove is formed in the hemispherical head; the rotating ball is rotationally arranged in the hemispherical head, and a limiting block matched with the limiting groove is fixedly connected to the rotating ball; according to the device, the mutual contact efficiency of raw materials is improved, the situation that the temperature of the raw materials on the inner wall of the fermentation tank is high after long-time heating is effectively prevented, meanwhile, the heating uniformity of the raw materials in the fermentation tank is effectively improved, and then the fermentation efficiency of traditional Chinese medicine is effectively improved.

To provide a manufacturing system and method for creating multiple tissue constructs from cells.SOLUTION: A system can include a thaw subsystem (if the cells are provided in a frozen state), an expansion subsystem, a concentration subsystem, and a tissue maturation subsystem. Each of these subsystems is modular and can be reconfigured, and a process can be repeated depending on the specific tissue process being implemented. Multiple tissue types can be combined in multiple bioreactors. The activities of multiple bioreactors can be coordinated and controlled in an automated manner by a supervisor controller. The supervisor controller can receive user input at the start of the process, and can manage the process thereafter, alerting the user if user actions are required.SELECTED DRAWING: None

The invention relates to the field of biological devices, in particular to a blood culture bottle positive sample transfer device which comprises a connecting pipe and at least two puncture needles, the puncture needles are communicated with an opening of the connecting pipe, a protective cover is arranged on the outer wall of the connecting pipe, and the protective cover is arranged on the outer wall of the connecting pipe. And the puncture needle close to the protective cover is arranged in the protective cover. A connecting seat is arranged at one end of the connecting tube, the protective cover is arranged on the outer wall of the connecting seat, and the puncture needle is communicated with the connecting tube through the connecting seat. And the protective cover is in threaded connection with the connecting seat. The device has the advantages that the blood sample in the culture bottle can be conveniently transferred, and pollution and biological hazards to the blood sample are avoided as much as possible.

The invention discloses an efficient denitrification method and system for landfill leachate, and the method comprises the following steps: diluting waste molasses, and then adding a compound enzyme preparation containing cellulase, pectinase and alpha-amylase for enzymolysis pretreatment to obtain a pretreatment solution; inoculating the pretreatment liquid with a compound flora containing clostridium butyricum and succinic acid-producing anaerobic spirillum, carrying out anaerobic fermentation, and separating to obtain a liquid carbon source containing acetic acid and propionic acid and fermentation residues; mixing the fermentation residues with waste sugar residues, and performing pyrolysis to prepare biochar; and synchronously adding the liquid carbon source and the biochar into a denitrification reactor, and carrying out denitrification on the landfill leachate. The total nitrogen removal load of the landfill leachate denitrification system is increased by 60% compared with that of a single carbon source process, and the sugar manufacturing solid waste recycling rate gt is achieved; 95%. According to the method disclosed by the invention, the dual goals of efficient denitrification and solid waste recycling are achieved through cooperative treatment of the waste molasses and the waste sugar residues in the sugar manufacturing process.

Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.

The invention relates to the technical field of tumor biomedical engineering and organ chips, in particular to a vascularized gastric cancer organ chip and a preparation method thereof.The chip is composed of an integrated micro-fluidic main body, an annular micro-column array and an optical sealing film, and a central culture cavity is divided into a tumor area and a blood vessel area by micro-columns; the preparation method comprises the following steps: injecting a fibrous protein solution containing human umbilical vein endothelial cells and cancer-related fibroblasts into a vascular region, and adding thrombin for in-situ gelation to form a pre-vascularized network; the method comprises the following steps: mixing a patient-derived gastric cancer organ with a methacrylic acid esterified gelatin pre-polymerized solution, injecting the mixture into a tumor area, and carrying out photo-crosslinking immobilization; culturing for 7-14 days under the dynamic perfusion condition of a mixed culture medium to obtain a three-dimensional gastric cancer organ model containing a capillary network; the method is expected to be used for rapid, low-cost and high-throughput screening of chemotherapy or anti-angiogenesis drugs and accurate prediction of individual curative effects of patients.

The invention discloses a mine microalgae carbon sequestration emission reduction and resource recycling system. The system comprises eight modules including a microalgae culture and closed pipeline photobioreactor microalgae culture module, a CO2 capturing and conveying module and a microalgae after-harvesting and processing module. The prepared culture medium and CO2 captured by an air return way are injected into a closed pipeline photobioreactor through a conveying pipeline, and the closed pipeline photobioreactor is provided with various sensors. Monitoring data are uploaded to the intelligent data analysis and optimization module for analysis and processing, and solution filling and environment parameters are intelligently adjusted through the intelligent monitoring and automatic control module; and collecting combustible gas generated by anaerobic fermentation of the microalgae, processing the primarily screened microalgae, and filtering the mine wastewater. According to the method, efficient culture of mine microalgae, efficient emission reduction and resource utilization of CO2 in a mine return airway and cyclic utilization of wastewater can be achieved, the green and low-carbon sustainable development concept is met, and a new solution is provided for the technical field of mine carbon dioxide emission reduction.

The present disclosure provides a sample extraction device, which includes a plurality of chambers arranged on a pedestal. The chamber includes a plurality of first chambers and two mixing chambers. The pedestal is internally provided with a first passage for communicating the two mixing chambers, and a sample extraction chamber is arranged in the first passage. The pedestal is further internally provided with a second passage for transfer and export of a liquid, and connection or disconnection between the two mixing chambers and the second passage is controlled by a valve. Sample extraction processes of the above technical solutions are carried out in a fully enclosed device, avoiding contamination of samples and extraction reagents by external environments, and avoiding environmental contamination caused by aerosols generated during the sample extraction processes. The sample extraction device is particularly suitable for extraction of highly pathogenic samples.

Methods and systems for performing a bioproduction process are described, the bioproduction process comprising the production of a transformed cell population. The methods include the steps of culturing a cell population at least partially embedded in a hydrogel matrix forming a three-dimensional structure, the hydrogel matrix also comprising a transforming agent.

A cell culture system includes: a plurality of cell culture channels each of which includes a fluidic device in which cells are cultured, a pump which makes a liquid flow into the fluidic device, a detection unit which detects a state of the cells that are cultured, and a controller which controls the pump and the detection unit; a measurement unit that measures culture environment of the cells which are cultured in the plurality of cell culture channels; and an information processing apparatus that adjusts the culture environment such that an absolute value of a difference between evaluation values for the states of the cells that are cultured in the plurality of cell culture channels is a threshold value or less, on the basis of the state of the cells detected by the detection unit and the culture environment of the cells measured by the measurement unit.