1878results about "Biological material testing procedures" patented technology

A tool for movement sequence analysis utilizing printed circuits. The tool determines a workflow sequence based, at least in part, on one or more printed circuits within a printed organic component. The tool activates the workflow sequence utilizing applied external stimuli on the one or more printed circuits. Responsive to activating the workflow sequence, the tool analyzes a signal response from the one or more printed circuits. The tool generates one or more printing recommendations based, at least in part, on the signal response analysis.

According to the device and the reagent for extracting the pathogen, the pathogen product separated from the uncultured sample extracted by using the device and the reagent can be subjected to a drug sensitivity test after simple culture and bacteria collection treatment, so that the culture process is shortened compared with pathogen diagnosis and identification of a clinical infected sample, and a drug sensitivity result can be obtained more quickly; and a more timely diagnosis and treatment reference is provided for clinicians.

The invention discloses an intelligent liquid supplementing and sampling system and method of a microorganism sampler and an unmanned flight system. A control module controls a sampling liquid pump to inject sampling liquid in a sampling liquid bottle into a wet-wall cyclone type sampler by a preset liquid injection amount and suspends the sampling liquid pump; the control module controls the fan to inject air into the wet-wall cyclone sampler for sampling, and adjusts the rotating speed of the fan in real time according to the flow detected by the sampling flow sensor, so that the sampling flow is maintained in a preset flow range; in the sampling process, the temperature sensor is used for detecting the temperature of the current air environment in real time per second, the humidity sensor is used for synchronously detecting the humidity of the current air environment in real time per second, and the control module is used for substituting the current temperature and the current humidity into a one-second sampling liquid evaporation capacity fitting function. And calculating the evaporation capacity of the sampling liquid in one second in the current temperature and humidity environment, controlling the working liquid supplementing time of the sampling liquid pump, and injecting the sampling liquid into the wet-wall cyclone type sampler to realize intelligent real-time liquid supplementing.

The present invention relates to a microfluidic device (1) for forming a cell aggregate comprising at least one first cell (C1) and one second cell (C2), and for individually processing selected cells of the cell aggregate: - Microfluidic channel (10); - At least one main inlet (11) for fluids containing a first cell, a second cell, and a third cell, respectively, located in the first portion (101) of the microfluidic channel; - An outlet (12) located in the second portion (102) of the microfluidic channel for controlling the flow rate of fluid within the microfluidic channel; - A first auxiliary inlet (131) for at least one first auxiliary fluid, located in the first portion (101) of the microfluidic channel upstream or downstream of the main inlet (11); - At least one cell trapping section (14) positioned between the first and second parts within the microfluidic channel; - At least one first valve to control the flow rate of the first auxiliary fluid, causing the fluid containing the first cells and the second cells to flow at a predetermined height within the microfluidic channel, thereby guiding the first cells and the second cells to the first and second capture units, respectively. Equipped with, The microfluidic device relates to a microfluidic device in which each cell capture section (14) comprises at least one first capture section (141) and one second capture section (142), each first and second capture section being sized to accommodate a first or second cell, the first and second capture sections being adjacent to each other in a direction perpendicular to the bottom (100) of the microfluidic channel, and forming a cell aggregate containing the captured first and second cells, each cell being at a different height relative to the bottom of the microfluidic channel.

Provided are a method and system for enriching natural killer (NK) cells in a blood sample.

The invention discloses an air microorganism aerosol alarm sampling system and method and an unmanned aerial vehicle system. A main controller receives a current position positioned by a GPS positioning module, and when the current position is in a to-be-detected area, an air pump and an alarm sampling optical module are started to pump air into the alarm sampling optical module; the alarm sampling optical module samples microbial particles in the air to form a fluorescence signal, the main controller analyzes the aerosol concentration according to the fluorescence signal and identifies whether the aerosol is a biological aerosol, when the aerosol concentration exceeds a set threshold value or the aerosol is identified as the biological aerosol, the alarm is controlled, and meanwhile, the sampling module is controlled to be started; the main controller controls the liquid supplementing peristaltic pump to start, the sampling liquid in the sampling liquid bottle is pumped into the sampler, then the motor driver is controlled to drive the fan to start to enable air to enter the sampler for sampling, only the sampling peristaltic pump is controlled to start after sampling is completed, and the sample liquid in the sampler is conveyed into the sample bottle through the three-way pipe. The collection of the microbial aerosol particles is completed.

Modular mucosal tissue bioreactors combined with bioartificial mucus provide a high-fidelity, medium-throughput platform for evaluating CF treatments and modeling host-pathogen biology, a stepping stone towards better options for HEMT-nonresponsive patients. Whole lung ex vivo Cystic Fibrosis model system allows for investigation of candidate CF therapies.

The present invention provides a long-period high-pressure enrichment culture and online monitoring apparatus and method for deep-sea microorganisms. The apparatus can simulate extreme deep-sea environments such as high pressure, low temperature, and oligotrophic conditions. Through pre-culture and graded serial culture, stable long-period growth and reproduction of the deep-sea microorganisms are ensured. An online system is used for monitoring the abundance, activity, and culture conditions of the microorganisms in real time to determine an appropriate timing for strain subculturing, and timely interactive subculturing and culture are performed, thereby achieving stable supply of high-abundance, high-activity microorganism enrichment culture solution. The present invention achieves precise pressure-retaining abundance counting of the microorganisms under in-situ pressure conditions and accurate control of a microbial growth cycle during enrichment culture, effectively improving timeliness and culture efficiency of the microorganism enrichment culture solution, and enabling long-period acquisition of high-abundance, high-activity microbial strain liquid.

The invention discloses a modified microfiltration membrane for enriching biological particles and a preparation method and application thereof, and belongs to the technical field of biological separation.The preparation method of the modified microfiltration membrane comprises the following steps that a mixed cellulose ester microfiltration membrane is provided and subjected to alkali liquor activating treatment; placing the activated membrane in a weakly alkaline buffer solution for balancing; the preparation method comprises the following steps: in a weakly alkaline buffer solution, firstly contacting the surface of the membrane with a tannic acid solution to carry out a first reaction, and then directly adding a polyethyleneimine solution to carry out a second reaction under the condition of not replacing a system, so as to form a phenol-amine cross-linked network on the surface of the membrane in situ; sodium cyanoborohydride is continuously added for reduction stabilization treatment, and the modified microporous filter membrane is obtained. The modified microporous filter membrane prepared by modifying the microporous filter membrane with tannic acid and polyethyleneimine can realize one-step synchronous enrichment of all negatively charged targets, and the problems of target loss, efficiency loss and the like caused by a multi-step process are avoided from the source.

An integrated modular microphysiological system is provided which includes a two or more chambers and a vascular network which includes at least one channel. The chamber can be configured for culturing a tissue and includes a layer of endothelial cells which forms an endothelial barrier within the well. The endothelial barrier can be in fluid contact with at least one of the at least one channels in the vascular network. The endothelial barrier can also be in fluid contact with a fluid in the chamber.

The invention discloses a method suitable for extracting extracellular small vesicles in fresh water pearl shell tissue waste liquid, which comprises the following operation steps: (1) solid-liquid separation: slaughtering pearl shells, and filling slaughtering waste liquid into a waste liquid crude extraction device; (2) waste liquid crude extraction: performing multi-stage gradient filtration and gravity settling on the slaughtering waste liquid by using a waste liquid crude extraction device to obtain clarified waste liquid; (3) removing impurities: removing impurities for the first time and removing impurities for the second time; (4) concentration of the extracellular vesicles: concentrating the extracellular vesicles by using an ultra-speed centrifuge to obtain a high-concentration extracellular vesicle solution; and (5) preservation of extracellular vesicles: according to the final volume of the high-concentration extracellular vesicle solution obtained in the step (4), carrying out sample standardization through protein concentration, sub-packaging the sample, and carrying out long-term preservation in a low-temperature environment. The particle sizes of the extracted vesicles are concentrated, high-purity and complete extracellular small vesicles can be obtained, and the method is suitable for biomedical research and product development.

A method includes introducing a suspension including cells suspended in a cell culture medium through a feed port or a drain port into a cavity of a cell culture vessel, the suspension being in an amount sufficient to cover a gas permeable, liquid impermeable membrane positioned at a bottom of the cell culture vessel, the feed port being disposed through a surface of the cell culture vessel and configured to permit additional cell culture medium into the cavity, and the drain port being disposed through the surface of the cell culture vessel and configured to permit removal of the cells, cell culture medium, and used cell culture medium from the cavity, allowing the cells to settle on the gas permeable, liquid impermeable membrane by gravity, removing the used cell culture medium through the drain port and introducing the additional cell culture medium through the feed port such that a constant volume is maintained in the cell culture vessel until the cells expand to a desired cell density, wherein the removing and introducing are performed subsequent to allowing the cells to settle on the gas permeable, liquid impermeable membrane, resuspending the cells in the cell culture medium in the cell culture vessel, wherein the resuspending is performed after the desired cell density is attained, and removing the resuspended cells and the cell culture medium through the drain port.

The invention discloses a bioreactor multi-parameter adaptive control system and method based on machine learning, and belongs to the technical field of bioreactor intelligent control, and the bioreactor multi-parameter adaptive control system comprises a bioreactor hepatocyte culture unit, a data acquisition and control unit and a machine learning intelligent decision-making unit. And the data acquisition and control lower computer controls operation variables such as gas supply, sampling and perfusion in the culture unit execution mechanism, collects culture process data returned by the parameter detection module, constructs a data-driven cell state prediction model by utilizing machine learning, and optimizes model parameters and operation variables based on a prediction result to realize closed-loop control. The method can overcome the defect that there is no precise mechanism model for hepatic cell culture, and multi-parameter collaborative optimization can be realized only depending on process data, so that the state of the hepatic cells is sensed in real time, full-cycle optimization of cell viability and functions is realized, and the activity and density of hepatic cell culture are effectively improved.

Systems and methods of clarifying a liquid suspension at high speeds contained in a flexible bag is provided. The method includes nesting at least one flexible bag containing the liquid suspension within at least one bag adapter with the at least one inlet line and / or outlet line extending from the flexible bag to an exterior of the bag adapter. The method further includes at least partially enclosing the at least one inlet line and / or outlet line within a crown cavity defined by a crown assembly. The method further includes centrifuging the at least one bag adapter and the at least one flexible bag containing the liquid suspension in a centrifuge to produce a super-natant fluid and a pellet in the at least one flexible bag.

Provided herein are systems, devices, and methods for processing, analyzing, and classifying biological data sets and generation of cell profiles. The data sets may include multi-omic data. Some embodiments may include the use of machine learning in training a classifier of raw multi-omic data and incorporating system biology knowledge to understand cellular behavior and cell status at the biomolecular level.

The present invention provides an online purification and sampling and visualized automatic enrichment apparatus and method for deep-sea microorganisms. Through online sample pretreatment during microorganism sampling, automatic biological concentration and purification, online monitoring of environmental indicators during biological enrichment, online observation of biomass, and automatic enrichment and transfer of high-purity enriched materials, the present invention overcomes the shortcomings of the prior art, such as difficulties in purifying deep-sea microorganism samples under high-pressure environments, complex manual operations for enrichment and observation, low timeliness of biomass observation, and difficulties in real-time cell counting of the enriched materials. This effectively increases the enrichment rate and acquisition efficiency of difficult-to-culture deep-sea microorganisms, providing a fundamental way for enhancing deep-sea biological research and bioresource development efficiency.

The disclosed invention provides a cartridge, method and cartridge processing systems for molecular tests that include extracting one or more biological materials from a tissue. The cartridge includes a base having a receptacle inside the base, a lid placed over the receptacle, and a gasket in the base. The base includes a transparent window placed under the receptacle, and the receptacle contains a slide on which the tissue is disposed. A film is disposed on an inner surface of the lid, and the film is suitable to extract the one or more biological materials from the tissue. The gasket surrounds the slide, and the lid creates a sealed chamber around the gasket that encloses the slide, and vacuum suction is applied through a port of the base to press the film against the tissue on the slide.

The utility model relates to a full-automatic all-in-one machine for NGS library building hybridization sequencing interpretation, which is used for sample adding and sequencing of a sequencing chip, and comprises a rack, a manipulator, a library preparation instrument and a sequencing device, the sequencing device comprises a gene sequencer, a sample adding clamping mechanism and a turnover mechanism, the library preparation instrument is used for sample adding of the sequencing chip, and the sample adding clamping mechanism is used for sample adding of the sequencing chip. The sample adding and clamping mechanism comprises a clamping tool and a clamping base, the clamping base is provided with a second positioning groove, the clamping tool is placed in the second positioning groove, the clamping tool clamps the sequencing chip, the overturning mechanism overturns the clamping tool to drive the sequencing chip for sample adding to overturn, the manipulator comprises an execution end and a clamping piece, the clamping piece is arranged at the execution end, and the clamping piece is arranged at the execution end. The clamping piece clamps the clamping tool, and the execution end moves among the library preparation instrument, the turnover mechanism and the gene sequencer, so that the sequencing chip is inserted into a preset position of the slot after being subjected to sample adding and turnover. The clamping tool clamps the sequencing chip, so that the sequencing chip is prevented from being overturned and falling off, automatic insertion of the sequencing chip is realized, and the sequencing efficiency is improved.

A lysis capsule for performing a cell lysis procedure includes a hollow body having an open first end and an open second end, a first porous membrane covering the open first end, and a second porous membrane covering the open second end, and the hollow body defines a lysis chamber between the first and second porous membranes. A plurality of non-magnetic beads and at least one magnetic element are disposed within the lysis chamber, and the pores of the first and second porous membranes are sized to retain the non-magnetic beads and the magnetic element within the lysis chamber. An internal control may be contained within the lysis chamber to validate an assay result and / or to validate the effectiveness of the cell lysis procedure. The lysis capsule may be disposed within a sample chamber of a fluidic cartridge.

The invention relates to the technical field of bacterial detection, in particular to a bacterial drug sensitivity detection assembly based on an ATP luciferase method and a using method.The bacterial drug sensitivity detection assembly comprises a supporting assembly, a sampling assembly, an ATP fluorescence detector and a 96-hole drug sensitivity plate, and a sealing block is arranged at the top of the inner wall of the ATP fluorescence detector; the supporting assembly comprises a base, a controller is arranged on one side of the base, and an electric push rod is arranged in the middle of the upper surface of the base. The device effectively realizes detection of bacterial drug sensitivity of a sample, is high in detection precision and good in detection effect, can also mix and stir the sample in the 96-hole drug sensitive plate, and avoids precipitation of bacteria in the 96-hole drug sensitive plate due to long-time standing; the sampling quantity of samples can be accurately detected, and the accuracy and the stability of subsequent detection are ensured; when the sample in the sampling tube is mixed with the reaction liquid, the flowing efficiency of the sample can be ensured, and the problems of high liquid feeding difficulty, poor mixing effect and the like caused by negative pressure are avoided.

According to the trace pathogen enrichment device and the detection method provided by the invention, pathogen products separated from uncultured samples extracted by using the device can be subjected to drug sensitivity test after simple culture and bacteria collection treatment, so that the culture process is shortened compared with pathogen diagnosis and identification of clinical infected samples, a drug sensitivity result can be obtained more quickly, and the detection efficiency is improved. And a more timely diagnosis and treatment reference is provided for clinicians.

The invention relates to the technical field of biological experiments, in particular to a strain sampling body, a transfer device and a method. The strain sampling body comprises a connecting part and a sampling part, the connecting part drives the strain sampling body to move through a mechanical arm, and the sampling part is used for dipping strains on the surface from a culture medium and moving into a nutrient solution to sink into the bottom to form a growth channel; the strain transfer device comprises a culture dish, a deep hole plate and a mechanical arm. The invention also provides a strain sampling and transferring method. According to the invention, a surface contact mode is adopted, so that full contact with strains can be realized; the density is higher than the concentration of a nutrient solution, and a growth channel can be formed when sinking, so that a starting point is provided for strain growth, complete transfer of sampled strains is ensured, residual waste of the strains is avoided, and efficient and accurate sampling is realized; the strain sampling body has various forms, materials, surface states and the like; sampling and transferring tasks of strains are indirectly completed through the strain sampling body, and the requirements of different experiments for strain treatment are met.

The invention relates to the technical field of bioengineering, in particular to an exosome separation and concentration method with marine animal source tissue as a raw material. Comprising the following steps: freeze-drying and crushing a marine animal source tissue sample; performing sample enzymolysis; and removing impurities and concentrating. According to the method disclosed by the invention, the exosome in the cells is fully dissociated through freeze-dried powder crushing and composite enzymolysis processes, and the filtering speed of the sample is accelerated by virtue of ultrasonic oscillation, so that the rapid and high-purity separation and concentration of the exosome in the marine animal source tissue are realized.

The utility model discloses a small-scale test device for research on anaerobic fermentation of granular sludge. The small-scale test device comprises a fermentation bottle, a granular sludge feeding component, a granular sludge sampling component, an exhaust component and a stirring and mixing device, the granular sludge feeding and sampling component comprises a sample feeding pipe and a sampling pipe which penetrate through the sealing rubber plug of the fermentation bottle, the sample feeding pipe and the sampling pipe enter the fermentation liquid in the fermentation bottle, the feeding pipe is provided with a manual ball valve, and the sampling pipe is provided with a manual ball valve and a check valve. The exhaust component comprises an exhaust pipe, a manual ball valve and an air collecting bag, the exhaust port is connected with the air collecting bag, and a closed environment is kept. The stirring device comprises a stirring rotor and a magnetic stirrer which are arranged in the fermentation bottle, and fermentation liquor is kept uniform in the sampling process. According to the small-scale test device for the granular sludge anaerobic fermentation research, the difficulty in a granular sludge fermentation test can be solved, the device is simple to assemble, the sampling is uniform and sufficient, and the anaerobic environment is stable.

The invention relates to the technical field of cell manipulation and analysis, and relates to an automatic single cell continuous capturing and screening system and a use method thereof. Comprising a bias liquid storage module, a cell injection module, a micro-fluidic chip, a cell capturing module, a cell screening module and an imaging system, the bias liquid storage module, the cell injection module, the cell screening module and the cell capturing module are connected with the micro-fluidic chip; the bias liquid storage module is used for generating bias flow; the cell injection module is used for providing cells; the micro-fluidic chip is used for capturing and screening cells; the cell capturing module is used for capturing cells; the cell screening module is used for screening cells; and the imaging system is used for acquiring captured cell images and performing visual analysis. Micro-fluidic pipeline double-bias-flow stable cell flow sample introduction is designed, a high-speed electromagnetic valve is matched to realize rapid control of a gas path on cell capturing and screening channel fluid, and a high-speed camera is combined to analyze cell positions in different areas in real time, so that automatic single cell continuous capturing and screening are realized.

The utility model belongs to the technical field of cell culture, and particularly relates to an automatic cell collecting device which comprises a rack and a sealing cover arranged on the rack, a normally closed butt joint window is arranged on one side of the sealing cover, and the butt joint window can be connected with a culture box; a culture dish can be placed in the culture box; the first mechanical arm is arranged on the operation platform; the liquid collecting mechanism is arranged on the operation platform, the liquid input mechanism is arranged on the operation platform, a first cover opening assembly is further arranged beside the liquid input mechanism, and the centrifugal collecting mechanism is arranged on the operation platform. By arranging the butt joint window, the first mechanical arm, the liquid collection mechanism, the liquid input mechanism and the centrifugal collection mechanism, the cell collection process can be fully automatically completed, the automation degree is high, the risk of artificial secondary pollution is reduced, meanwhile, a production line can be synthesized with a cell inoculation device, the first mechanical arm is shared, the size of the machine is reduced, and the production cost is reduced. The occupied area is small, the cost is low, and the structure is simpler.

A treatment facility (1) for the treatment of fermentable solid products by fermentation has a reactor (2) supported by a frame and a cartridge (11) having an apertured region (110) for storing the product. The reactor (2) is provided with an inlet (3) for products to be treated, an outlet (4) for discharging the treated products, a biogas outlet (5), an opening (8) for inserting an inoculum, and an opening (9) for discharging the leachate. The facility also has a tubular body (6) and a liquid / solid separator (7). The body (6) is closed at both ends by a closing element (100) movable between an open position and a closed position. One of the ends (3) receives the products to be treated and the other of the ends (4) discharges the treated products, each cartridge (11) being slidably movable inside the body (6) and the liquid / solid separator (7) having a filter (12).

A cell culture device configured to cultivate cells, the culture device includes a plate with at least one well configured to receive at least one cell to be cultivated, a permeable support sheet, a fixation layer, configured to connect the plate to the permeable support sheet, the fixation layer presenting a predefined shape including at least one wall extending between the permeable support sheet and the plate. The fixation layer is designed in accordance with a pre-defined growing path, in order to model and shape the growth of the cultivated cells outside the well in a controlled and predetermined fashion.

The utility model discloses a device capable of feeding fungicide at high temperature and pressure equalizing, and belongs to the field of fungicide preparation. The device comprises a base, a protection ring is arranged at the upper end of the outer portion of the base, and a machining ring is arranged in the protection ring in a surrounding mode, and the lower end of the machining ring is fixedly welded to the upper end of the base. The utility model solves the problems that the inner wall of the equipment can be kept clean through the rubbing of a brush when the existing automatic fed-batch microbial inoculum device is used, but the inner wall cannot be completely cleaned only through the rubbing of the brush, and only the rubbing position of the brush on the inner wall cannot generate the retention of foreign matters; in order to solve the problems that in the prior art, the device cannot be unfolded and completely clean the internal structure, rotation of a threaded rod is matched with limitation of a limiting rod on a protection ring, the longitudinal horizontal position of the protection ring can be adjusted, the protection ring can be unfolded and completely exposed out of the upper end of the outer portion of a machining ring when moving upwards in the longitudinal direction, and cleaning is convenient.