4717results about "Biological material testing procedures" patented technology

Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.

Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.

Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.

The present disclosure provides systems and methods for processing cells. The systems of the present disclosure may include a compartment, a three-dimensional (3D) matrix, a fluid flow path, an analysis unit, a preservation unit, or any combination thereof. The methods of the present disclosure may process cells via, for example, expansion, culturing, gene delivery, activation, or any combination thereof. The systems and methods described herein may be useful for producing cell products including, for example, cell therapy products.

The invention discloses a maltodextrin waste residue treatment device and method.The maltodextrin waste residue treatment device comprises a fermentation tank, a stirring shaft, a stirring paddle, a first driving mechanism and an air guide bin, the interior of the stirring shaft is hollow to form a first air channel, the interior of the stirring paddle is hollow to form a second air channel, the first air channel is communicated with the second air channel, and a plurality of aeration holes are formed in the stirring paddle; a connecting rod, a spring and a plurality of blocking blocks provided with air passing holes are arranged in the second air channel, the blocking blocks are connected in series through the connecting rod, the spring pulls the connecting rod to block the aeration holes through the blocking blocks, a pull rope is arranged in the first air channel, one end of the pull rope is fixedly connected with the connecting rod, the pull rope pulls the connecting rod to move horizontally, and the air guide bin is installed at the top of the stirring shaft. The air guide bin is rotationally connected with the stirring shaft, an air inlet pipe is arranged on the air guide bin, and an air inlet hole is formed in the stirring shaft. According to the method, the maltodextrin waste residues are subjected to pulse feed fermentation, the ineffective oxygen supply proportion is reduced, the problem of waste of a large amount of energy is solved, the environment is protected, and enterprise benefits are improved.

A reinforcing and scaling construction for a bioprinted tissue model, which comprises a casing and sealing bioink, the casing comprising an inner module comprising a casing base (1) and a perforated cover (2), and an outer module comprising a container (3) comprising technological valves (5), an outer cover (4) and plugs (6) for technological valves (5). The invention also relates to a method for assembling the reinforcing and sealing construction.

The invention belongs to the technical field of inspection and extraction, and particularly relates to an automatic T lymphocyte suction device which comprises an automatic suction device body, the automatic suction device body comprises a bearing table, a rotating disc with a circumferential rotating function is arranged on the bearing table, and a plurality of centrifugal assemblies are evenly distributed on the rotating disc. A centrifugal tube feeding assembly facilitating feeding of centrifugal tubes is arranged in front of one side of the bearing table, a tube carrying and conveying assembly is arranged on the portion, located on one side of the centrifugal tube feeding assembly, of the bearing table, a tube feeding mechanism is arranged at the end of the conveying belt mechanism, and a reaction reagent adding assembly is arranged on one side of the tube carrying and conveying assembly. A sample feeding assembly is arranged on one side of the upper portion of the workbench, and a first suction assembly is arranged above the multi-direction adjusting assembly. According to the invention, multiple samples can be processed in batches, the working efficiency and the cell extraction purity are improved, the influence of manual operation on the result is reduced, the accuracy of the detection effect is ensured, and the use requirements are met.

The invention discloses a probiotic fermentation device, and relates to the technical field of fermentation devices, the probiotic fermentation device comprises a fermentation tank, a tank cover is mounted on the fermentation tank, a fixed plate is fixedly connected to the inner wall of the fermentation tank, a gas collection plate is slidably connected to the inner wall of the fermentation tank close to the upper part of the fixed plate, and a ventilation shaft is rotatably connected to the middle part of the tank cover; the middle part of the gas collecting plate is slidably connected to the outer surface of the ventilation shaft, and the outer surface of the middle part of the ventilation shaft is rotatably connected to the fixed plate; compared with the prior art, the device has the advantages that the ventilation shaft rotates under the action of driving force, the ventilation stirring plate can be driven to move together, comprehensive stirring operation is carried out on mixed thalli in the fermentation container, uniform distribution of the thalli is promoted in the stirring process, the contact opportunity of the thalli and oxygen is greatly increased, and the fermentation efficiency is improved. Therefore, the fermentation rate and efficiency of the probiotics are obviously improved.

The invention discloses convenient-to-adjust shaking-up equipment for cell culture, and relates to the technical field of cell culture. The shaking-up device comprises a shaking-up plate which is obliquely arranged in a shell, a plurality of holes are formed in the surface of the shaking-up plate, a limiting assembly is fixedly connected to the inner side face of the shaking-up plate, a driving assembly is fixedly connected to the bottom of the inner side face of the shell, and the side, close to an end cover, of the driving assembly is in spline clamping connection with the surface of the limiting assembly. The clamping assembly is fixedly connected with the inner wall of the hole of the shaking plate, the shaking plate is obliquely arranged, the culture bottle not only generates horizontal circular motion in the rotating process, but also generates up-and-down displacement along with the inclined surface of the shaking plate, and the culture bottle is subjected to a complete ascending and descending process in the process of revolving around the driving assembly for a circle; a three-dimensional composite oscillation effect is formed, so that a culture medium and a culture bottle are promoted to generate complex relative movement, the contact between cells and nutrient substances can be more efficiently promoted, and the gas exchange efficiency is enhanced.

The invention relates to the technical field of deep-sea microorganism nucleic acid detection, in particular to a long-term experimental platform for deep-sea microorganism in-situ nucleic acid detection and a detection method. According to the invention, a water sample is subjected to tangential flow filtration and concentration through the filtration and sampling module, so that a concentrated microorganism sample is obtained. The method comprises the following steps of: transferring a sample into an amplification detection module under reduced pressure, transferring into a lysis purification module for cell lysis, injecting the cracked sample into a chromatographic column, filtering impurities and intercepting DNA by the chromatographic column, then washing by using an eluent to obtain a high-purity DNA sample, loading a suction head through a transfer washing liquid gun, mixing the high-purity DNA sample with an enzyme solution, adding into the amplification detection module for reaction, and detecting the DNA in the amplification detection module. And carrying out fluorescence labeling on the amplified gene, obtaining an amplification curve by using a fluorescence detector, and detecting the in-situ nucleic acid of the deep-sea microorganisms according to the change of the amplification curve. An automatic cleaning assembly is designed in the filtering and sampling module, and the cracking and purifying module is used for sampling and cleaning a cracking chamber and switching pipelines, so that cross contamination caused by deep sea in-situ multiple detection is prevented.

To provide a manufacturing system and method for creating multiple tissue constructs from cells.SOLUTION: A system can include a thaw subsystem (if the cells are provided in a frozen state), an expansion subsystem, a concentration subsystem, and a tissue maturation subsystem. Each of these subsystems is modular and can be reconfigured, and a process can be repeated depending on the specific tissue process being implemented. Multiple tissue types can be combined in multiple bioreactors. The activities of multiple bioreactors can be coordinated and controlled in an automated manner by a supervisor controller. The supervisor controller can receive user input at the start of the process, and can manage the process thereafter, alerting the user if user actions are required.SELECTED DRAWING: None

The invention discloses an efficient denitrification method and system for landfill leachate, and the method comprises the following steps: diluting waste molasses, and then adding a compound enzyme preparation containing cellulase, pectinase and alpha-amylase for enzymolysis pretreatment to obtain a pretreatment solution; inoculating the pretreatment liquid with a compound flora containing clostridium butyricum and succinic acid-producing anaerobic spirillum, carrying out anaerobic fermentation, and separating to obtain a liquid carbon source containing acetic acid and propionic acid and fermentation residues; mixing the fermentation residues with waste sugar residues, and performing pyrolysis to prepare biochar; and synchronously adding the liquid carbon source and the biochar into a denitrification reactor, and carrying out denitrification on the landfill leachate. The total nitrogen removal load of the landfill leachate denitrification system is increased by 60% compared with that of a single carbon source process, and the sugar manufacturing solid waste recycling rate gt is achieved; 95%. According to the method disclosed by the invention, the dual goals of efficient denitrification and solid waste recycling are achieved through cooperative treatment of the waste molasses and the waste sugar residues in the sugar manufacturing process.

Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.

The invention relates to the technical field of tumor biomedical engineering and organ chips, in particular to a vascularized gastric cancer organ chip and a preparation method thereof.The chip is composed of an integrated micro-fluidic main body, an annular micro-column array and an optical sealing film, and a central culture cavity is divided into a tumor area and a blood vessel area by micro-columns; the preparation method comprises the following steps: injecting a fibrous protein solution containing human umbilical vein endothelial cells and cancer-related fibroblasts into a vascular region, and adding thrombin for in-situ gelation to form a pre-vascularized network; the method comprises the following steps: mixing a patient-derived gastric cancer organ with a methacrylic acid esterified gelatin pre-polymerized solution, injecting the mixture into a tumor area, and carrying out photo-crosslinking immobilization; culturing for 7-14 days under the dynamic perfusion condition of a mixed culture medium to obtain a three-dimensional gastric cancer organ model containing a capillary network; the method is expected to be used for rapid, low-cost and high-throughput screening of chemotherapy or anti-angiogenesis drugs and accurate prediction of individual curative effects of patients.

A cell culture system includes: a plurality of cell culture channels each of which includes a fluidic device in which cells are cultured, a pump which makes a liquid flow into the fluidic device, a detection unit which detects a state of the cells that are cultured, and a controller which controls the pump and the detection unit; a measurement unit that measures culture environment of the cells which are cultured in the plurality of cell culture channels; and an information processing apparatus that adjusts the culture environment such that an absolute value of a difference between evaluation values for the states of the cells that are cultured in the plurality of cell culture channels is a threshold value or less, on the basis of the state of the cells detected by the detection unit and the culture environment of the cells measured by the measurement unit.

The invention discloses an inertial micro-fluidic chip integrating cell focusing and sorting and a preparation method thereof. The inertial micro-fluidic chip comprises a cover plate layer, a runner layer and a substrate layer, a chip inlet, a first chip outlet and a second chip outlet are formed in the cover plate layer; the flow channel layer is provided with a sample liquid flow channel inlet, a first flow channel outlet and a second flow channel outlet, the chip inlet is communicated with the sample liquid flow channel inlet, the first chip outlet is communicated with the first flow channel outlet, and the second chip outlet is communicated with the second flow channel outlet. The flow channel layer is provided with a spiral cell sorting flow channel and a cell focusing flow channel, the front end of the spiral cell sorting flow channel is communicated with a sample liquid flow channel inlet, the tail end of the spiral cell sorting flow channel is communicated with the cell focusing flow channel and a first flow channel outlet, and the tail end of the cell focusing flow channel is communicated with a second flow channel outlet. Through a microfluid vortex focusing technology and a spiral flow channel inertia sorting technology, accurate sorting and focusing of cells are realized, and the problems that the focusing state of the sorted cells is influenced and the cell counting detection precision is low are effectively solved.

An exosome extraction device, comprising: a base (4); a filter column placement disc (1), wherein the filter column placement disc (1) comprises a placement disc body (11), a plurality of extraction column placement holes (111) are formed in the placement disc body (11), the extraction column placement holes (111) are used for placement of exosome extraction columns (100), and the placement disc body (11) can be rotatably mounted on the base (4) relative to the base (4); an exosome collection disc (2), wherein the exosome collection disc (2) comprises a collection disc body (21), a plurality of collection tube placement holes (211) are formed in the collection disc body (21), the collection tube placement holes (211) are used for placement of exosome collection tubes (200), and the collection disc body (21) is rotatably mounted on the base (4) relative to the base (4); and a collection disc translation mechanism for driving the collection disc body (21) to move to a position below the placement disc body (11) or move away from the position below the placement disc body (11), wherein in the state where the collection disc body (21) is located below the collection disc body (11), the placement disc body (11) and the collection disc body (21) rotate, so that the collection tube placement holes (211) can be aligned with the extraction column placement holes (111) so as to collect exosomes dripping from the exosome extraction columns (100).

A tool for movement sequence analysis utilizing printed circuits. The tool determines a workflow sequence based, at least in part, on one or more printed circuits within a printed organic component. The tool activates the workflow sequence utilizing applied external stimuli on the one or more printed circuits. Responsive to activating the workflow sequence, the tool analyzes a signal response from the one or more printed circuits. The tool generates one or more printing recommendations based, at least in part, on the signal response analysis.

A sorting track for sorting living sperm includes a housing that defines at least two cubicles, an initial placement location, and a channel fluidly coupled to the at least two cubicles and the initial placement location such that the at least two cubicles each feature a unique set of cubicle characteristics, an extraction portal, and an entrance fluidly coupled to the channel. Cubicle characteristics may include one or more architectural characteristics selected from the group consisting of: a distance from the initial placement location, a cubicle shape, a cubicle volume, a cubicle position within the housing relative to the channel, an entrance size, and an entrance shape. Cubicle characteristics may also include stimulus characteristics selected from the group consisting of: a wavelength of light, a light intensity, a radioactive radiation intensity, an electromagnetic field, a chemical treatment, a pressure, a sound, and a temperature.

Apparatuses and methods for microscopic analysis of a sample by simultaneously characterizing infrared absorption characteristics of a plurality of spatially resolved locations are described herein. These apparatuses and methods improve sampling times while collecting microscopic data regarding composition of a sample across a wide field.

The invention discloses a soil microbial fermentation device and a fermentation method, and relates to the field of microbial fermentation. A soil microorganism fermentation device comprises a fermentation tank and further comprises a cover plate detachably connected to the upper end of the fermentation tank; the material conveying barrel is positioned in the fermentation tank and is rotationally connected to the cover plate; the rotating sleeve is connected to the material conveying barrel in a sleeving mode, and a shifting rod is fixedly connected to the rotating sleeve; the motor drives the bevel gear for transmission, the linkage conveying barrel and the rotating shaft to rotate, and the electric telescopic rod is matched to drive the rotating sleeve to reciprocate, so that the three-dimensional stirring of the driving lever and the circulating conveying of the spiral blade are realized, a multi-dimensional stirring mode is formed, the material mixing uniformity is improved, the full contact of microorganisms and nutrient substances is promoted, and the fermentation efficiency and quality are improved; the elastic deflector rod and the counterweight ball can adaptively adjust the stirring range along with the rotating speed, eliminate stirring dead angles, accelerate the fermentation process, shorten the period, reduce the cost, and further optimize the fermentation effect and the product quality.

The utility model provides an automatic filling device and a biological tissue transferring and filling device, and the automatic filling device comprises one or more negative pressure ports which are arranged on one wall of the automatic filling device, the one or more negative pressure ports are connected with liquid or air to be sucked through one or more sleeves; and the negative pressure device is used for filling liquid or air into the one or more sleeves through the one or more negative pressure ports. According to the casing pipe filling device, the casing pipe filling efficiency is effectively improved, and the casing pipe filling complexity is reduced.

The invention relates to the technical field of biological medicine preparation equipment, and discloses an intelligent CAR-T cell culture system, which comprises an intelligent temperature control and cleaning box body, a double sterilization system and a full-automatic preparation process, and the intelligent temperature control and cleaning box body adopts temperature and humidity control and cleanliness control. The double sterilization system comprises hydrogen peroxide fumigation and ultraviolet circulation disinfection, and the full-automatic preparation process comprises a precise pipeline, a modular reaction unit and an automatic control platform. According to the intelligent CAR-T cell culture system, full-process automatic culture of full-automatic CAR-T cells is realized in a C-level environment, a high-requirement clean environment is not needed, the cost can be greatly reduced, the economic benefit is remarkable, a full-closed pipeline system better meets the requirements of laws and regulations, and the system has greater advantages in the supervision level; the full-automatic operation reduces the intervention of personnel, avoids the potential pollution risk in the process, the full-process automatic cultivation can greatly save the cost, and the cost is saved by 40% compared with the traditional process through comprehensive calculation.

According to the device and the reagent for extracting the pathogen, the pathogen product separated from the uncultured sample extracted by using the device and the reagent can be subjected to a drug sensitivity test after simple culture and bacteria collection treatment, so that the culture process is shortened compared with pathogen diagnosis and identification of a clinical infected sample, and a drug sensitivity result can be obtained more quickly; and a more timely diagnosis and treatment reference is provided for clinicians.

The present invention provides a method and system for fabricating complex human tissues by integrating bio-electrospraying, cell electrospinning merged with 3D multi material microfluidic bioprinting, and future additions of magnetic and acoustic levitation technologies. The fabricated tissues serve as an alternative to animal testing in drug discovery processes, aiding in the prediction of human physiological responses to various drug compounds.

The invention discloses rapid extraction equipment for cell collection and a use method of the rapid extraction equipment, and belongs to the technical field of cell collection and extraction.The rapid extraction equipment is characterized in that a sample is sucked into a turnover cylinder, a sliding plate is supported through elasticity of a second spring, a sealing seat can be tightly attached to a conical groove, and a liquid inlet pipe is automatically blocked to prevent backflow of the sample; the piston column moves downwards so that samples can enter the guide pipes through the one-way valves, the one-way valves can prevent the samples from flowing back into the turnover cylinder, the multiple one-way valves communicate with the turnover cylinder, in the downward pressing process of the piston column, filling can be conducted on the multiple centrifugal tubes at the same time, and the samples in the turnover cylinder are divided through the multiple guide pipes; therefore, the quantitative sampling and extracting work can be conveniently and rapidly completed by collecting the total amount of the sample at a time and then distributing the sample into the multiple centrifugal tubes, the sample in the turnover cylinder does not flow back, and cross contamination is prevented.

An apparatus for separating cell suspension material into centrate and concentrate, includes a single use structure (178, 240, 250, 370, 414) releasably positioned in a cavity in a solid wall rotatable centrifuge bowl (172). The bowl and portions of single use structure rotate about an axis (174, 428). A stationary inlet feed tube (184, 430), a centrate discharge tube (212, 436) and a concentrate discharge tube (230,448) extend along the axis of the rotating single use structure. A centrate centripetal pump (208, 438) is in fluid connection with the centrate discharge tube. A concentrate centripetal pump (216, 450) is in fluid connection with the concentrate discharge tube. At least one concentrate channel (380, 454) and a concentrate centripetal pump chamber (376,452) have configurations in the structure that facilitate the flow of cell concentrate.

A holder apparatus, which is placed on a stage of an observation apparatus including the stage, an observation optical system which is arranged below the stage and on which light from a biological sample is incident, and a control unit, includes: a holder body which holds a culture vessel in which the biological sample is arranged; and an illumination optical system which is provided in the holder body and illuminates the biological sample, and the illumination optical system is arranged in at least one of a side surface portion or an upper portion of the holder body when a direction in which the biological sample is observed is set as a vertical direction.