71 results about "Patch clamp" patented technology

The invention discloses a patch clamp electrophysiological data processing method and a patch clamp electrophysiological data processing system. The method comprises the following steps: acquiring ion channel current data through a patch clamp microelectrode; baseline drift removal processing is carried out on the ion channel current data through an adaptive quadratic linear fitting function to obtain baseline correction current data; performing discretization processing and high-frequency filtering processing on the baseline correction current data based on a preset sampling frequency to obtain a filtering current signal sequence; performing calculation through a false nearest neighbor method to obtain an optimal embedding dimension, performing calculation based on an average displacement method to obtain optimal delay time, and performing second phase space reconstruction through the filtering current signal sequence, the optimal embedding dimension and the optimal delay time to obtain an optimal vector set and a phase space reconstruction matrix; calculating through a singular value decomposition algorithm and a Shannon formula to obtain a principal component vector set and an information entropy corresponding to each vector, judging through a preset information entropy threshold to obtain a principal vector sequence, and reconstructing through a singular matrix to obtain electrophysiological feature data.

The invention discloses a single vesicle particle electrochemical signal identification and classification method and identification system, and the method comprises the steps: S1, preparing a carbon fiber ultramicro electrode, applying a voltage in a patch clamp system constant potential mode, and collecting picoampere-level current response generated by oxidation of a single vesicle content on the surface of the electrode; s2, carrying out smooth filtering on the collected original current signal, and realizing characteristic peak identification and multi-dimensional physical / statistical characteristic extraction by using a multi-parameter peak identification method based on a threshold value; s3, carrying out preprocessing operations including missing value processing, standardization, principal component analysis dimension reduction and category equalization on the extracted peak value characteristic data; and S4, on the basis of the preprocessed feature data, constructing and training at least one machine learning model for automatically identifying the particle size category of the vesicle particles. The method combines the advantages of single-vesicle electrochemical high-sensitivity measurement and intelligent algorithm classification, and has the advantages of high recognition efficiency, good repeatability, wide applicability and the like.

The invention provides a biosensor based on heterostructure double holes and a preparation method and application thereof. The device comprises a silicon nitride chip fixed between two electrolyte-filled liquid storage tanks, a layer of graphene film is transferred on the silicon nitride chip in advance, one nanopore is etched on a graphene interface, and the other nanopore is etched on a silicon nitride interface. The two side liquid pools are respectively connected with the positive electrode and the negative electrode of the patch clamp amplifier. When a certain potential difference is applied to the two electrodes, the biological macromolecules in the liquid pool can be captured into the nanometer channel. When two ends of a molecule are captured by two different nanopores at the same time, due to different interaction between a silicon nitride-graphene heterogeneous interface and the molecule and the action of electric field force, the molecule can form a dragsaw phenomenon between the two pores, and the time of the molecule passing through the nanopores is prolonged. According to the invention, the rate of chain molecules such as DNA, protein and the like passing through the nanopore can be regulated and controlled, and accurate control of single molecules and even gene sequencing can be realized.

The present invention relates to the technical field of microbial pharmaceutical chemistry, specifically to a method for preparing a Penicillium fungus ( Penicillium daleae The present invention relates to the medical use of a polyketide compound with a unique cage-like structure isolated from the fermentation product of a strain of the bacterium Mycobacterium spp. Using patch-clamp techniques to measure the action potential discharge frequency and spontaneous excitatory post-synaptic currents (sEPSCs) of hippocampal basket cells, the present invention confirmed that the compound has significant inhibitory activity on neuronal excitability and is non-toxic to N2a neurons at active concentrations. The compound can be used to prepare new drugs for treating diseases associated with neuronal hyperexcitability.

The utility model discloses an ultrasonic probe combined patch clamp device which comprises a placing table and a patch clamp bearing base, the patch clamp bearing base is arranged at the top of the placing table, and the patch clamp bearing base is used for placing a culture dish; the ultrasonic probe is arranged above the patch clamp bearing base; the regulation and control mechanism is connected with the ultrasonic probe and the placement table, and the regulation and control mechanism is used for regulating the position and the angle of the ultrasonic probe. An ultrasonic technology and a patch clamp technology can be combined by utilizing the patch clamp bearing base and the ultrasonic probe, so that the ultrasonic technology and the patch clamp technology can be used at the same time, operation steps during an experiment are reduced, the difficulty of experiment operation is greatly reduced, the ultrasonic technology and the patch clamp technology can be used in a combined manner, and the experiment efficiency is improved. More comprehensive cell and molecular biology research is carried out, and the occurrence mechanism of diseases is deeply understood.

The utility model discloses a detection device for detecting the thickness of a patch, and relates to the technical field of patch detection. The patch conveying device comprises a base plate, a patch moving assembly is arranged on the base plate and comprises a conveying belt arranged above the base plate, a driving roller and a driven roller are in transmission connection with the two ends of the interior of the conveying belt, and the lower side of a circular gear connected with the end of the driving roller is in meshed connection with a missing gear. The end of a rotating shaft connected with the side face of the missing gear is connected with a motor. A patch clamping assembly is arranged on the outer side wall of the conveying belt, and a placement frame in the patch clamping assembly is connected to the outer side wall of the conveying belt. According to the patch thickness detection device, the patch moving assembly is arranged, the motor can be conveniently controlled to drive patch bodies to move at equal time intervals, then the patch bodies can be conveniently subjected to thickness detection one by one, the operation steps are simplified, the patch clamping assembly is arranged, the patch bodies to be detected are clamped and positioned, and the stability of the patch bodies during thickness detection is improved.

The present invention provides a patch clamp probe for testing material state density and its use method. The patch clamp probe integrates the electrolyte, electrolytic cell, counter electrode, and reference electrode used in conventional micro-nano energy storage on-chip devices for electrochemical testing. A three-dimensional motion device is used to move the patch clamp probe close to the surface site of the micro-nano energy storage on-chip device to be tested. This not only simplifies the preparation process of the micro-nano energy storage on-chip device but also enables characterization of the electrochemical state density of different active sites on the same micro-nano energy storage on-chip device.

A method and system for differentiating acute and chronic pain based on the functional state of specific neural circuits is proposed. This method utilizes in vivo two-photon / endoscopic calcium imaging and ex vivo brain slice patch-clamp techniques to longitudinally monitor the activity dynamics of neurons in the DRN and ZI brain regions during the acute and chronic phases of a neuropathic pain model. By extracting and analyzing calcium and electrophysiological signal parameter sets, distinguishing criteria are established: if the activity of 5-HTergic neurons in the DRN is specifically enhanced in the acute phase, the indication is acute pain; if the activity of GABAergic neurons in the ZI is specifically enhanced in the chronic phase, the indication is chronic pain. This invention achieves an objective and precise differentiation of the transition from acute to chronic pain at the level of specific neural circuits, providing a new strategy and tool for pain mechanism research and clinical diagnosis and treatment.

1. Name of the design product: Perfusion device with adjustable speed for patch clamp. 2. Use of the design product: The design product is used to control the perfusion speed during the preparation of patch clamp. 3. Design key points of the design product: Lies in the shape. 4. Picture or photograph that best shows the design key points: Perspective view 1.

The utility model discloses a novel SMT paster clamp, comprising a rack, the bottom surface of the rack is provided with a positive and negative tooth lead screw through a bearing pedestal, one end of the positive and negative tooth lead screw is provided with a driven gear, the top surface of the rack is fixedly provided with a servo motor, the output end of the servo motor is provided with a driving gear, and the driving gear is provided with a driven gear. The driving gear is meshed with the driven gear, a moving block is used on the positive and negative tooth lead screw in a matched mode, a sliding block is welded to the top face of the moving block, a sliding groove is formed in the surface of the rack, the sliding block is in sliding connection with the sliding groove, and a supporting seat is welded to the top end of the sliding block; a support and a supporting table are fixedly connected to the upper end and the lower end of the surface of one side of the supporting base correspondingly. According to the SMT patch clamping device, the clamping plate for elastically clamping the SMT patch is arranged, a buffering process can be achieved in the clamping process of the SMT patch, the SMT patch can be prevented from being damaged by impact force in cooperation with use of the protection pad, it is guaranteed that the SMT patch cannot be damaged, practicability is high, and the SMT patch clamping device is worthy of popularization.

The invention relates to the technical field of gene detection devices, in particular to a silicon nitride nanopore device and a preparation method and application thereof based on an angstrom hole, a helium ion beam is used for irradiating the surface of a silicon nitride film of a cleaned silicon nitride chip to obtain a silicon nitride chip with an angstrom hole, and the silicon nitride chip is used for detecting the gene detection device. And after cleaning and drying, carrying out dielectric breakdown to obtain a nanopore with a required size, measuring the obtained nanopore again by using the patch clamp, and drying by using nitrogen to obtain the silicon nitride nanopore device. According to the silicon nitride nanopore device and the preparation method and application thereof based on the angstrom pore, the generation position of the nanopore is controlled in a helium ion beam irradiation area through an angstrom pore confinement intrinsic carrier effect and an avalanche effect and breakdown critical defect density correlation mechanism; and then the formation, evolution and size regulation and control of the nanopore are tracked in real time through dielectric breakdown ion current, and controllable manufacturing of the nanopore with the extremely small size is achieved.

The utility model provides a patch clamp constant temperature device with good temperature control performance, which relates to the technical field of patch clamps and comprises a base, an adjusting mechanism is arranged at the top end of the base, a constant temperature heater body is arranged at the bottom of the base, and a square groove is formed in the top end of the base close to the center. A square groove is formed in the top end of the base, a first sliding groove is formed in the top end of the base and located on the front face of the square groove, an electric sliding block is arranged in the first sliding groove, a workbench is arranged at the top end of the electric sliding block, and limiting grooves are symmetrically formed in the top end of the base and located on the left side and the right side of the square groove and the first sliding groove. Limiting blocks are arranged at the positions, corresponding to the limiting grooves, of the bottom of the workbench, a groove is formed in the top end of the workbench in a penetrating mode, and the workbench can be driven to slide front and back through an electric sliding block, so that workers can place and take diaphragms conveniently, and the working efficiency is effectively improved; and the working table can be isolated from the outside through the heat insulation cover, so that the practicability is improved.

The invention discloses a method for detecting a retinal ganglion cell ion channel and a glaucoma rat model used by the method. The method comprises the following steps: detecting an ion channel of a retinal ganglion cell through a patch clamp; the retinal ganglion cells are prepared by the following steps: sequentially performing incubation, digestion and cell dispersion on the retina of a glaucoma rat with a complete optic nerve papilla under an oxygen introduction condition; the digestion refers to digestion in papain digestive juice, the papain digestive juice comprises Hank's liquid, papain with the concentration of 10-50 [mu] g / mL, L-cysteine with the concentration of 0.5-1 mg / mL and BSA with the concentration of 0.5-1 mg / mL, and the specific activity of the papain is larger than or equal to 10 U / mg. According to the method disclosed by the invention, an in-vitro manual patch clamp is used for carrying out retinal ganglion cell ion channel detection on a magnetic bead induced intraocular hypertension rat, and a good foundation is laid for subsequent glaucoma mechanism research.

This application provides a nanoprobe-based electrophysiological signal and biochemical biomarker detection system, comprising: a patch-clamp amplifier; a working electrode electrically connected to a signal acquisition module, the surface of which, except for the tip, is covered by an insulating protective layer, forming a GΩ-level impedance seal after implantation into the cell; a counter electrode connected to a BATH port, with its active end placed in the matrix environment of the target cell, forming a current loop; a reference electrode connected to a REF port, with its active end placed in the matrix environment of the target cell to provide a potential reference point; the patch-clamp amplifier is configured to operate in voltage-clamp mode, with the REF port connected to the reference electrode as a voltage sensing terminal, and the BATH port connected to the counter electrode as a current output terminal, simultaneously acquiring the electrophysiological signal of the target cell and the current signal corresponding to the concentration of the target biochemical biomarker. This application provides a powerful analytical tool for a deeper understanding of the electrochemical signal coupling mechanism of neurons.

The utility model discloses a patch clamp perfusion device, which comprises a control module, telescopic electric push rods and a fixed support, the control module comprises a multi-loop time controller and a continuously variable transmission, the fixed support simultaneously fixes a plurality of injectors, one telescopic electric push rod is arranged above a push rod of each injector, and the telescopic electric push rods are connected with the fixed support. A needle cylinder head of each injector corresponds to a needle cylinder head flow guide pipe, each needle cylinder head flow guide pipe is connected with a pipeline, and all the pipelines are converged to a needle head; the telescopic electric push rods are electrically connected with the control module, and under the control of the multi-loop time controller and the continuously variable transmission, the telescopic electric push rods mechanically compress the injector push rods, so that medicine in the injector needle cylinder reaches the needle head through a pipeline. According to the device, regular and constant-speed perfusion administration can be realized; the whole injector can be replaced, a pipeline is convenient to clean, and cross contamination is avoided; meanwhile, the whole device is high in integration degree, small in size, small in occupied space, simple in structure, short in pipeline and free of medicine waste.

The invention relates to a nucleic acid detection system based on glass nanopores and nanomanipulation as well as a preparation method and application of the nucleic acid detection system, and belongs to the technical field of biology. Aiming at the technical problems of difficulty in signal capture, incapability of realizing accurate space positioning and insufficient complex structure detection accuracy caused by too high nucleic acid translocation speed in the existing nanopore technology, a DNA chip is combined with a nanomanipulation technology. A modified glass chip of which the surface is fixed with double Gap double-stranded DNA is constructed, a glass nanopore is matched, an electric field is applied by utilizing a patch clamp system, and a piezoelectric ceramic nano displacement platform is combined to realize accurate control on a DNA structure. According to the method, fixed-point capture, multiple repeated detection and accurate distinguishing of sequences with different lengths of nucleic acid are realized, the detection limitation of a traditional nanopore technology on a complex nucleic acid structure is solved, and the method has important application value in the fields of gene diagnosis and nanopore sequencing.

The invention provides a screening method of a targeted Nedd4-2 intermediary hERG potassium channel degradation small-molecule inhibitor and the inhibitor thereof. According to the invention, through a molecular docking virtual screening technology, cell culture, plasmid transfection, an FLIPR ion channel screening technology, a Western Blot technology and a patch clamp technology, a method for high-throughput screening of a targeted Nedd4-2 intermediate hERG potassium channel degradation small molecule inhibitor is established. The screened compound LACCAIC ACID A (number 17) can reduce the degradation of hERG potassium channel on the basis of overexpression of Nedd4-2, increase Ikr current, obviously antagonize the extension of repolarization time history caused by ET-1, and reduce the occurrence rate of cell arrhythmia. The results show that the LACCAIC ACID A compound obtained by the screening method is competitively combined with the PY sequence of the hERG channel with Nedd4-2, so that the ubiquitination degradation of the hERG channel by the Nedd4-2 is reduced, and a research and development basis is provided for further developing hERG potassium channel degradation prevention and treatment drugs.

The invention discloses an application of a total alkaloid extract of herba aconiti in preparation of a Kv4.3 channel inhibitor, and belongs to the technical field of biological medicines. According to the method disclosed by the invention, the blocking of Kv4.3 current by the total alkaloid extract of the herba aconiti is represented in detail by utilizing a whole-cell patch clamp technology. It is found for the first time that the herba aconiti total alkaloid extract is an effective direct blocker of heart A-type channel Kv4.3 current, and ventricular repolarization of an in-vitro rat is remarkably delayed. So far, there is no detailed research on interaction between the total alkaloid extract of the herba aconiti and a heart Kv4.3 channel, a new insight is provided for a potential mechanism that the total alkaloid extract of the herba aconiti blocks A-type current, the possibility is provided for developing the total alkaloid extract of the herba aconiti into a novel Kv4.3 current inhibitor tool medicine, and the application prospect is wide.

The utility model discloses an SMT patch buckling jig, and relates to the technical field of SMT patch processing equipment, the SMT patch buckling jig comprises a first jig (1) and a second jig (2), the first jig (1) is arranged above the second jig (2), the first jig (1) is provided with a first fixing groove (4), the second jig (2) is provided with a second fixing groove (5), and the first fixing groove (4) and the second fixing groove (5) are arranged on the first jig (1). A first fixing groove (4) and a second fixing groove (5) are formed in the first jig body (1), the first fixing groove (4) and the second fixing groove (5) are communicated to form a concave shape, a circular groove (8) is formed in the first jig body (1), a fixing mechanism (6) is arranged in the circular groove (8), and a locking block (7) is arranged on the first jig body (1). And the problem that the jig cannot be used is solved.

The invention relates to the technical field of biosensors, and particularly discloses a C reactive protein composite nanopore sensor for slowing down the nucleic acid pore passing rate and a preparation method of the C reactive protein composite nanopore sensor. According to the method, a solid nanopore chip with a silicon nitride suspended film with the thickness of 30 nm is installed in a fluid pool, and nanopores with the diameter of 8-10 nm are formed in the silicon nitride suspended film; adding an electrolyte solution with the pH value of 7-9 into a Cis side chamber and a Trans side chamber of the fluid pool; two Ag / AgCl electrode wires are respectively inserted into a Cis side chamber and a Trans side chamber, and the other two ends of the electrode wires are respectively connected with patch clamp current amplifiers; positive and negative voltages of 200 mV-200 mV are applied to the Trans side of the fluid pool, and a current value is recorded as a negative tapping current; adding a C reactive protein solution with the final concentration of 10-100 nM to the Cis side of the fluid pool; meanwhile, negative voltage of-50 mV to-300 mV is applied to the Trans side, the C-reactive protein composite nanopore sensor is obtained when it is observed that long-time current is smaller than negative pore opening current, and the DNA pore passing speed reduction effect of nearly two orders of magnitude can be achieved.

The invention discloses a method for separating cochlea related cells in situ based on a patch clamp system. The method comprises the following steps: contacting an electrode with a target single cell under a microscope, and carrying out in-situ grabbing on the target single cell through negative pressure adsorption; the target single cell and surrounding cells are kept in a connection state before in-situ grabbing; before the electrode approaches the target single cell, positive pressure is continuously applied, and when the electrode is in contact with the surface of the target single cell, the positive pressure is instantly stopped and switched to negative pressure. According to the method provided by the invention, high-quality cochlea related cells can be obtained in situ for deep sequencing analysis, and the method can be used for basic research and accurate diagnosis and treatment of clinical hearing impairment, and has a good application prospect.

The utility model relates to the field of biological medicine experimental equipment, and discloses a precise perfusion dosing device for patch clamp experiments, which comprises an equipment seat, a fixing column is fixedly connected to the middle of the top end of the equipment seat, and a fixing plate is fixedly connected to the rear side of the top of the outer wall of the fixing column. Electric telescopic rods are fixedly connected to the left side and the right side of the rear portion of the top end of the fixing plate, a concave clamping plate is fixedly connected to one ends of the two electric telescopic rods, and a limiting clamping plate is fixedly connected to the front side of the top of the outer wall of the fixing column. According to the utility model, by opening the piston I, the liquid medicine flows into the gathering box due to gravity and is added to a tissue sample, the concave clamping plate and the plug rod of the electric telescopic rod are started to rise, the syringe generates suction force, the medicine in various medicine boxes enters the four-way pipe through the feeding pipe and the one-way valve I and reaches the syringe, and the piston I is closed, and the electric telescopic rod shrinks and recovers to the initial position; and medicines can be automatically supplemented and added.

The utility model relates to the technical field of clamping equipment, in particular to a storage device for a patch clamp glass electrode holder, which comprises a base, a fixing rod and an opening and closing assembly, the opening and closing assembly comprises a rotating plate rotationally connected with a placing groove, the bottom surface of the rotating plate is rotationally connected with a supporting plate, and the rotating plate can rotate relative to the supporting plate. A hole is formed in the middle of the supporting plate, a limiting block is fixedly installed on the supporting plate, a sliding groove is formed in the rotating plate, the limiting block is slidably arranged in the sliding groove, the rotating plate is rotationally connected with a connecting rod, and the connecting rod is rotationally connected with a baffle. According to the storage device for the patch clamp glass electrode holder, the rotating plate is rotated to drive the connecting rod to move and the baffle to move, so that the hole is opened and closed, the patch clamp glass electrode holder is isolated from air, the patch clamp glass electrode holder is prevented from being in contact with dust in the air, and the service life of the patch clamp glass electrode holder is prolonged. And the glass electrode holder of the patch clamp is prevented from getting dirty.

The application provides a content extraction method based on cell membrane capacitance change, and belongs to the field of cell-level micromanipulation technology, and comprises the following steps: S1, a change relationship between cell membrane capacitance and cell membrane surface area is established; S2, a change relationship between cell membrane surface area and volume is approximately established; S3, high-resistance sealing and membrane rupture of cells are completed, constant voltage cell content extraction operation is performed, and cell membrane capacitance values in the extraction process are obtained in real time through capacitance fitting; S4, content extraction amount is estimated; S5, actual cytoplasm extraction amount is calculated based on a rotary L-shaped electrode, the relationship model established in S2 is corrected, and finally, quantitative extraction of cell contents is realized. The application utilizes an electrical model of cell membrane capacitance, realizes real-time feedback of cell content extraction amount, realizes quantitative control of content extraction, can realize a cytoplasm extraction success rate of 62.5% in the case of a whole cell patch clamp structure, and the average cytoplasm extraction amount control error is less than 6% of the total cell volume.

The invention discloses a patch clamp-based regulation and control analysis method and a patch clamp-based regulation and control analysis system for electrical stimulation induced tunnel nanotube mediated cell electrical coupling, and relates to the technical field of cell biology. The patch clamp-based regulation and control analysis method for electrical stimulation induced tunnel nanotube mediated cell electrical coupling comprises the following steps: S1, sample preparation and drug intervention: adding at least one signal path inhibitor into a cell bath solution for intervention; s2, performing membrane potential imaging and data analysis; s3, applying electrical stimulation and coupling verification: applying electrical stimulation in a specific mode to one cell in the tunnel nanotube connection cell pair by using patch clamp equipment, and executing membrane potential imaging and data analysis; s4, measuring the traction force of the cells; s5, analyzing membrane potential change and traction force change; through accurate electrical stimulation and multi-parameter monitoring, the regulation and control mechanism of tunnel nanotube mediated cell electrical coupling is deeply analyzed, and an effective means is provided for research of intercellular signal transmission.

This invention discloses a split-type noise reduction and vibration damping optical platform for patch clamp experiments, comprising: a buoyancy tank, a base plate platform, and an operating platform; a movable base plate is provided at the inner bottom of the buoyancy tank, and the circumferential side of the movable base plate abuts against the inner wall of the buoyancy tank to form a seal; a damping spring is provided on the base plate platform, penetrating the bottom of the buoyancy tank; a bent rod is fixedly provided on the bottom side of the movable base plate, one end of the bent rod is hinged to a vertical rod, and one end of the vertical rod is hinged to a swing arm; the end of the swing arm is provided with an active tooth that rotates with it; a driven tooth is provided on a second support rod, and a linkage rod is provided on one side of the driven tooth; one end of the linkage rod is fixedly connected to the movable base plate, and the active tooth and rack respectively mesh with the driven tooth. This invention enables the movable base plate and the sealing plate to be in a linked state, allowing the fluid in the divided cavity to directly enter / exit the second hole, while the volume of fluid remaining in the first hole remains unaffected, ensuring the stability of the float position in the first hole and avoiding interference from signal noise during the experiment.

1. The name of the design product: sensor patch clamp (adjustable pressure combined type). 2. The use of the design product: used for the pressurization and curing process of the patch process in the manufacturing process of the weighing load sensor. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view.

The utility model provides an SMT patch clamp, and relates to the technical field of SMT patch clamps. The SMT patch clamp comprises a base, a driving motor is fixed to one side of the base, a two-way threaded rod is fixed to the driving end of the driving motor, threaded blocks are in threaded connection with the outer surfaces of the two sides of the two-way threaded rod, and fixing blocks are fixed to the upper ends of the two threaded blocks; a connecting structure is arranged on the close sides of the two fixing blocks, and two extrusion frames and two pressing frames are arranged on the connecting structure. The connecting structure can drive the two extrusion frames to move reversely in the length direction of the base and can drive the two pressing frames to move in the height direction of the base at the same time. According to the SMT clamp, the driving motor drives the bidirectional threaded rod to rotate, so that the effect of conveniently and simultaneously clamping in two directions is achieved, the effect of improving the use convenience of the SMT clamp is achieved, the effect of removing dust by blowing is also achieved, and the effect of improving the use effect of the SMT clamp is achieved.

The present invention relates a composition comprising an extract of Fomitopsis pinicola as an active ingredients for the treatment or prevention of ischemic brain diseases and the use thereof. The inventive extract of Fomitopsis pinicola showed potent treating effect on ischemic brain disease through various experiments, for example, (1) ASIC1 ion channel inhibition assay using a patch clamp (Experimental Example 1) on the extract of Fomitopsis pinicola of the present invention; and neuroprotective effect analysis experiment using a neuroblastoma cell line (sh-sy5y) to inhibit ASIC1 and inhibit apoptosis, and the results of these experiments confirm that the inventive extract of the present invention can be used as a treatment or adjuvant for ischemic brain disease, and confirm that the composition is useful as a pharmaceutical composition, health functional food and dietary supplement for the prevention or treatment of ischemic brain disease.