36 results about "Patch clamp" patented technology

The invention discloses a single vesicle particle electrochemical signal identification and classification method and identification system, and the method comprises the steps: S1, preparing a carbon fiber ultramicro electrode, applying a voltage in a patch clamp system constant potential mode, and collecting picoampere-level current response generated by oxidation of a single vesicle content on the surface of the electrode; s2, carrying out smooth filtering on the collected original current signal, and realizing characteristic peak identification and multi-dimensional physical / statistical characteristic extraction by using a multi-parameter peak identification method based on a threshold value; s3, carrying out preprocessing operations including missing value processing, standardization, principal component analysis dimension reduction and category equalization on the extracted peak value characteristic data; and S4, on the basis of the preprocessed feature data, constructing and training at least one machine learning model for automatically identifying the particle size category of the vesicle particles. The method combines the advantages of single-vesicle electrochemical high-sensitivity measurement and intelligent algorithm classification, and has the advantages of high recognition efficiency, good repeatability, wide applicability and the like.

The invention provides a biosensor based on heterostructure double holes and a preparation method and application thereof. The device comprises a silicon nitride chip fixed between two electrolyte-filled liquid storage tanks, a layer of graphene film is transferred on the silicon nitride chip in advance, one nanopore is etched on a graphene interface, and the other nanopore is etched on a silicon nitride interface. The two side liquid pools are respectively connected with the positive electrode and the negative electrode of the patch clamp amplifier. When a certain potential difference is applied to the two electrodes, the biological macromolecules in the liquid pool can be captured into the nanometer channel. When two ends of a molecule are captured by two different nanopores at the same time, due to different interaction between a silicon nitride-graphene heterogeneous interface and the molecule and the action of electric field force, the molecule can form a dragsaw phenomenon between the two pores, and the time of the molecule passing through the nanopores is prolonged. According to the invention, the rate of chain molecules such as DNA, protein and the like passing through the nanopore can be regulated and controlled, and accurate control of single molecules and even gene sequencing can be realized.

A method and system for differentiating acute and chronic pain based on the functional state of specific neural circuits is proposed. This method utilizes in vivo two-photon / endoscopic calcium imaging and ex vivo brain slice patch-clamp techniques to longitudinally monitor the activity dynamics of neurons in the DRN and ZI brain regions during the acute and chronic phases of a neuropathic pain model. By extracting and analyzing calcium and electrophysiological signal parameter sets, distinguishing criteria are established: if the activity of 5-HTergic neurons in the DRN is specifically enhanced in the acute phase, the indication is acute pain; if the activity of GABAergic neurons in the ZI is specifically enhanced in the chronic phase, the indication is chronic pain. This invention achieves an objective and precise differentiation of the transition from acute to chronic pain at the level of specific neural circuits, providing a new strategy and tool for pain mechanism research and clinical diagnosis and treatment.

The utility model discloses a novel SMT paster clamp, comprising a rack, the bottom surface of the rack is provided with a positive and negative tooth lead screw through a bearing pedestal, one end of the positive and negative tooth lead screw is provided with a driven gear, the top surface of the rack is fixedly provided with a servo motor, the output end of the servo motor is provided with a driving gear, and the driving gear is provided with a driven gear. The driving gear is meshed with the driven gear, a moving block is used on the positive and negative tooth lead screw in a matched mode, a sliding block is welded to the top face of the moving block, a sliding groove is formed in the surface of the rack, the sliding block is in sliding connection with the sliding groove, and a supporting seat is welded to the top end of the sliding block; a support and a supporting table are fixedly connected to the upper end and the lower end of the surface of one side of the supporting base correspondingly. According to the SMT patch clamping device, the clamping plate for elastically clamping the SMT patch is arranged, a buffering process can be achieved in the clamping process of the SMT patch, the SMT patch can be prevented from being damaged by impact force in cooperation with use of the protection pad, it is guaranteed that the SMT patch cannot be damaged, practicability is high, and the SMT patch clamping device is worthy of popularization.

The invention relates to the technical field of gene detection devices, in particular to a silicon nitride nanopore device and a preparation method and application thereof based on an angstrom hole, a helium ion beam is used for irradiating the surface of a silicon nitride film of a cleaned silicon nitride chip to obtain a silicon nitride chip with an angstrom hole, and the silicon nitride chip is used for detecting the gene detection device. And after cleaning and drying, carrying out dielectric breakdown to obtain a nanopore with a required size, measuring the obtained nanopore again by using the patch clamp, and drying by using nitrogen to obtain the silicon nitride nanopore device. According to the silicon nitride nanopore device and the preparation method and application thereof based on the angstrom pore, the generation position of the nanopore is controlled in a helium ion beam irradiation area through an angstrom pore confinement intrinsic carrier effect and an avalanche effect and breakdown critical defect density correlation mechanism; and then the formation, evolution and size regulation and control of the nanopore are tracked in real time through dielectric breakdown ion current, and controllable manufacturing of the nanopore with the extremely small size is achieved.

This application provides a nanoprobe-based electrophysiological signal and biochemical biomarker detection system, comprising: a patch-clamp amplifier; a working electrode electrically connected to a signal acquisition module, the surface of which, except for the tip, is covered by an insulating protective layer, forming a GΩ-level impedance seal after implantation into the cell; a counter electrode connected to a BATH port, with its active end placed in the matrix environment of the target cell, forming a current loop; a reference electrode connected to a REF port, with its active end placed in the matrix environment of the target cell to provide a potential reference point; the patch-clamp amplifier is configured to operate in voltage-clamp mode, with the REF port connected to the reference electrode as a voltage sensing terminal, and the BATH port connected to the counter electrode as a current output terminal, simultaneously acquiring the electrophysiological signal of the target cell and the current signal corresponding to the concentration of the target biochemical biomarker. This application provides a powerful analytical tool for a deeper understanding of the electrochemical signal coupling mechanism of neurons.

The invention discloses an application of a total alkaloid extract of herba aconiti in preparation of a Kv4.3 channel inhibitor, and belongs to the technical field of biological medicines. According to the method disclosed by the invention, the blocking of Kv4.3 current by the total alkaloid extract of the herba aconiti is represented in detail by utilizing a whole-cell patch clamp technology. It is found for the first time that the herba aconiti total alkaloid extract is an effective direct blocker of heart A-type channel Kv4.3 current, and ventricular repolarization of an in-vitro rat is remarkably delayed. So far, there is no detailed research on interaction between the total alkaloid extract of the herba aconiti and a heart Kv4.3 channel, a new insight is provided for a potential mechanism that the total alkaloid extract of the herba aconiti blocks A-type current, the possibility is provided for developing the total alkaloid extract of the herba aconiti into a novel Kv4.3 current inhibitor tool medicine, and the application prospect is wide.

The invention relates to the technical field of biosensors, and particularly discloses a C reactive protein composite nanopore sensor for slowing down the nucleic acid pore passing rate and a preparation method of the C reactive protein composite nanopore sensor. According to the method, a solid nanopore chip with a silicon nitride suspended film with the thickness of 30 nm is installed in a fluid pool, and nanopores with the diameter of 8-10 nm are formed in the silicon nitride suspended film; adding an electrolyte solution with the pH value of 7-9 into a Cis side chamber and a Trans side chamber of the fluid pool; two Ag / AgCl electrode wires are respectively inserted into a Cis side chamber and a Trans side chamber, and the other two ends of the electrode wires are respectively connected with patch clamp current amplifiers; positive and negative voltages of 200 mV-200 mV are applied to the Trans side of the fluid pool, and a current value is recorded as a negative tapping current; adding a C reactive protein solution with the final concentration of 10-100 nM to the Cis side of the fluid pool; meanwhile, negative voltage of-50 mV to-300 mV is applied to the Trans side, the C-reactive protein composite nanopore sensor is obtained when it is observed that long-time current is smaller than negative pore opening current, and the DNA pore passing speed reduction effect of nearly two orders of magnitude can be achieved.

The invention discloses a method for separating cochlea related cells in situ based on a patch clamp system. The method comprises the following steps: contacting an electrode with a target single cell under a microscope, and carrying out in-situ grabbing on the target single cell through negative pressure adsorption; the target single cell and surrounding cells are kept in a connection state before in-situ grabbing; before the electrode approaches the target single cell, positive pressure is continuously applied, and when the electrode is in contact with the surface of the target single cell, the positive pressure is instantly stopped and switched to negative pressure. According to the method provided by the invention, high-quality cochlea related cells can be obtained in situ for deep sequencing analysis, and the method can be used for basic research and accurate diagnosis and treatment of clinical hearing impairment, and has a good application prospect.

The application provides a content extraction method based on cell membrane capacitance change, and belongs to the field of cell-level micromanipulation technology, and comprises the following steps: S1, a change relationship between cell membrane capacitance and cell membrane surface area is established; S2, a change relationship between cell membrane surface area and volume is approximately established; S3, high-resistance sealing and membrane rupture of cells are completed, constant voltage cell content extraction operation is performed, and cell membrane capacitance values in the extraction process are obtained in real time through capacitance fitting; S4, content extraction amount is estimated; S5, actual cytoplasm extraction amount is calculated based on a rotary L-shaped electrode, the relationship model established in S2 is corrected, and finally, quantitative extraction of cell contents is realized. The application utilizes an electrical model of cell membrane capacitance, realizes real-time feedback of cell content extraction amount, realizes quantitative control of content extraction, can realize a cytoplasm extraction success rate of 62.5% in the case of a whole cell patch clamp structure, and the average cytoplasm extraction amount control error is less than 6% of the total cell volume.

The invention discloses a patch clamp-based regulation and control analysis method and a patch clamp-based regulation and control analysis system for electrical stimulation induced tunnel nanotube mediated cell electrical coupling, and relates to the technical field of cell biology. The patch clamp-based regulation and control analysis method for electrical stimulation induced tunnel nanotube mediated cell electrical coupling comprises the following steps: S1, sample preparation and drug intervention: adding at least one signal path inhibitor into a cell bath solution for intervention; s2, performing membrane potential imaging and data analysis; s3, applying electrical stimulation and coupling verification: applying electrical stimulation in a specific mode to one cell in the tunnel nanotube connection cell pair by using patch clamp equipment, and executing membrane potential imaging and data analysis; s4, measuring the traction force of the cells; s5, analyzing membrane potential change and traction force change; through accurate electrical stimulation and multi-parameter monitoring, the regulation and control mechanism of tunnel nanotube mediated cell electrical coupling is deeply analyzed, and an effective means is provided for research of intercellular signal transmission.

This invention discloses a split-type noise reduction and vibration damping optical platform for patch clamp experiments, comprising: a buoyancy tank, a base plate platform, and an operating platform; a movable base plate is provided at the inner bottom of the buoyancy tank, and the circumferential side of the movable base plate abuts against the inner wall of the buoyancy tank to form a seal; a damping spring is provided on the base plate platform, penetrating the bottom of the buoyancy tank; a bent rod is fixedly provided on the bottom side of the movable base plate, one end of the bent rod is hinged to a vertical rod, and one end of the vertical rod is hinged to a swing arm; the end of the swing arm is provided with an active tooth that rotates with it; a driven tooth is provided on a second support rod, and a linkage rod is provided on one side of the driven tooth; one end of the linkage rod is fixedly connected to the movable base plate, and the active tooth and rack respectively mesh with the driven tooth. This invention enables the movable base plate and the sealing plate to be in a linked state, allowing the fluid in the divided cavity to directly enter / exit the second hole, while the volume of fluid remaining in the first hole remains unaffected, ensuring the stability of the float position in the first hole and avoiding interference from signal noise during the experiment.

1. The name of the design product: sensor patch clamp (adjustable pressure combined type). 2. The use of the design product: used for the pressurization and curing process of the patch process in the manufacturing process of the weighing load sensor. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view.

The utility model provides an SMT patch clamp, and relates to the technical field of SMT patch clamps. The SMT patch clamp comprises a base, a driving motor is fixed to one side of the base, a two-way threaded rod is fixed to the driving end of the driving motor, threaded blocks are in threaded connection with the outer surfaces of the two sides of the two-way threaded rod, and fixing blocks are fixed to the upper ends of the two threaded blocks; a connecting structure is arranged on the close sides of the two fixing blocks, and two extrusion frames and two pressing frames are arranged on the connecting structure. The connecting structure can drive the two extrusion frames to move reversely in the length direction of the base and can drive the two pressing frames to move in the height direction of the base at the same time. According to the SMT clamp, the driving motor drives the bidirectional threaded rod to rotate, so that the effect of conveniently and simultaneously clamping in two directions is achieved, the effect of improving the use convenience of the SMT clamp is achieved, the effect of removing dust by blowing is also achieved, and the effect of improving the use effect of the SMT clamp is achieved.

The invention discloses an in-vitro retina photostimulation device for retina patch clamp operation, and belongs to the field of optics and biomedical engineering. The device is mainly composed of four parts: a light source screen module can display a color programmable pattern; the screen mainboard is integrated with an accelerometer and a gyroscope, so that the screen posture can be measured; the convex lens is used for reducing a light source screen output pattern to a retina adaptive size; the dichroscope can reflect the shrunk pattern and transmit infrared light; the light path support is used for fixing the light source screen, the convex lens and the dichroscope, and enables the light source screen, the convex lens and the dichroscope to keep a preset distance so as to achieve an expected reduction magnification. Based on a convex lens imaging rule, the projection system can project a programmable image with 240 * 240 pixels and a single pixel side length of 10 microns on the retina, and can realize pattern correction according to a screen inclination angle.

The invention discloses a perforated patch clamp operation method based on particle diffusion control, which belongs to the field of micromanipulation and comprises the following steps: S1, establishing an initial geometric environment statistical model of perforated particles; s2, establishing a perforated particle diffusion model, and obtaining the number of perforated material particles diffused to a cell membrane at any time; s3, establishing a perforation reaction rate model to obtain the number of perforations in the cell membrane at any time; s4, designing a model prediction controller, and realizing perforation membrane rupture based on particle diffusion control by controlling the diffusion velocity of the perforation material; and S5, adjusting the particle diffusion speed according to a modeling result and a transmembrane current peak value in combination with an automatic operation process of the perforation patch clamp, so as to obtain an ideal perforation progress. By establishing the diffusion and reaction model of the perforation solution, the change relation of the perforation number corresponding to the perforation solutions with different concentrations along with the time is obtained, and the perforation process is controlled on the basis of the change relation.

The application discloses an automatic two-electrode patch clamp operation method, and relates to the technical field of cell-level micromanipulation, and specifically comprises the following steps: S1, calculating the working space of a two-electrode tip through patch clamp double-mechanical arm motion modeling; S2, acquiring the pixel point coordinates of the two needles in the field of view through a deep learning algorithm for two-dimensional positioning of the needle tip; S3, according to the double-electrode working space obtained in step S1, guiding the placement position of the brain slice and the method for positioning the needle tip in step S2, performing motion control on the double electrode in three stages of coarse-fine-precision by using a Qt Creator tool; and S4, moving and contacting the target cell surface of the double-electrode patch clamp through an automatic program control. The application automatically controls the double electrode to contact the target cell under the working space obtained based on double-arm kinematic modeling by using a deep learning target detection method, and realizes a contact success rate of 90% under an average operation time of 6 minutes.

The application discloses a single-vacuole particle electrochemical signal recognition classification method and a recognition system, and the method comprises the following steps: S1, a carbon fiber ultramicroelectrode is prepared, and a voltage is applied in a membrane patch clamp system constant potential mode, and a picoampere-level current response generated by oxidation of single-vacuole content on the surface of the electrode is collected; S2, the collected original current signal is subjected to smoothing filtering, and a multi-parameter peak recognition method based on a threshold value is used to realize feature peak recognition and multi-dimensional physical / statistical feature extraction; S3, the extracted peak feature data is subjected to a preprocessing operation including missing value processing, standardization, principal component analysis dimension reduction and class balancing; and S4, at least one machine learning model is constructed and trained based on the preprocessed feature data, and is used for automatically recognizing the particle size class of the vacuole particle. The application combines the advantages of single-vacuole electrochemical high-sensitivity measurement and intelligent algorithm classification, and has the advantages of high recognition efficiency, good repeatability and wide applicability.

The invention discloses a portable plant surface potential detection device and method, the device comprises a detection electrode assembly, a portable electrochemical workstation and a terminal computer, the detection electrode assembly is electrically connected with the electrochemical workstation through a shielding conductive wire, and the electrochemical workstation is in bidirectional communication connection with the terminal computer. Plant surface potential monitoring is carried out by creatively adopting an open circuit electrochemical method, traditional high-price patch clamp equipment is replaced with a low-cost portable electrochemical workstation, and all parts of the device are in portable modular design and can be quickly assembled and disassembled, so that the device is convenient to use and popularize. And the field real-time monitoring of the surface potential of plant stalks and leaves in a field natural growth state is realized. According to the device, under set detection and stimulation parameters, personal errors are effectively reduced, detection data are accurate, stability is high, potential response rules of plants under stimulation of fire, damage and the like can be captured, and the device has the advantages of being low in cost, portable, high in adaptability and wide in application prospect.

This invention discloses the application of a polypeptide toxin Mp3c derived from the spider *Gymnospermum tectorum*. Patch-clamp experiments verified that Mp3c effectively inhibits the voltage-gated potassium channel Kv1.3, with inhibitory effects approximately 3 times and 18 times greater than those of Kv1.4 and Kv1.5, respectively. This Kv1.3 polypeptide toxin inhibitor, as an immunosuppressant, specifically inhibits T cells involved in autoimmune diseases. EM Based on the strong inhibitory effect of Mp3c on Kv1.3, it is speculated that it may selectively inhibit T cells. EM The activation process plays a role, thus possessing therapeutic potential for autoimmune diseases. Furthermore, Mp3c has no significant inhibitory effect on other voltage-gated potassium channels, including Herg channels, which can, to some extent, avoid post-drug cardiotoxicity.

This invention provides a method for identifying compounds for treating delayed neurological injury in the chronic phase of cerebral hemorrhage. The method includes establishing a mouse model of cerebral hemorrhage, administering the identified drug to a treatment group and immunoglobulin IgG to a non-treatment group; assessing motor dysfunction through neurological function evaluation and a turning angle test; evaluating the excitability of thalamic neurons using whole-cell patch-clamp recording technology; comparing synaptic transmission function in the treatment and non-treatment groups; assessing spatial learning and memory abilities using the Morris water maze test; and comprehensively evaluating the improvement in neurological function, synaptic transmission, and cognitive function to determine the therapeutic effect of the drug. The method also includes collecting autopsy brain tissue from cerebral hemorrhage patients and healthy controls, performing spatial transcriptome sequencing to identify differentially expressed genes, and exploring the pathogenic mechanism in the animal model of cerebral hemorrhage. This method can accurately screen and identify potential therapeutic drugs for chronic neurological injury in cerebral hemorrhage.

The invention relates to an analysis method for mouse retinal ganglion cell morphological classification, which realizes single-cell-level accurate analysis and high-throughput integration: carrying out whole-cell patch post-clamp dendritic structure reconstruction on retinal ganglion cells, objectively quantifying parameters such as dendritic area, layering depth, dendritic asymmetry and the like, and analyzing the morphological classification of the mouse retinal ganglion cells. The subjective experience dependence of traditional Golgi staining and the resolution limitation of immunohistochemistry are thoroughly eliminated; a hierarchical classification rule is established through an automatic algorithm, manual interpretation deviation is eliminated, and the classification standardization degree is improved; the method breaks through the low-flux bottleneck of a genetic marker technology, does not need to depend on time-consuming gene model construction, synchronously analyzes the morphological diversity of cells through single cell sequencing and morphological reconstruction, and reveals that cells with similar dendritic layering positions can present functional differentiation due to transcriptome differences. The morphological analysis system established by the invention has single cell precision, high-throughput coverage and multi-dimensional relevance, and provides technical support for systematically analyzing the morphological development mechanism and subtype specificity of RGCs.

This invention discloses a method for efficient reconstruction of neuronal morphology based on a visual model. Addressing the problem of high noise and low contrast in patch-clamp stained neuronal images, which hinders automated reconstruction using existing methods, the method preprocesses the Z-axis tomographic images by grayscale conversion, frequency domain filtering, and multi-scale block segmentation. The SOLOv2 model is pre-trained using a wall seam dataset through transfer learning, and then fine-tuned using labeled neuronal data to identify slender branch structures. The segmentation results of each image block are concatenated to reconstruct a binary mask. The Z-axis masks are then superimposed, and isolated regions with fewer than three consecutive frames and a distance exceeding 70 pixels from the main skeleton are removed to obtain a three-dimensional spatially consistent mask. Finally, the width, height, maximum path distance of the cell body, and longest trunk length are automatically extracted using skeletonization and graph theory algorithms. On 207 mouse and macaque neurons, the correlation coefficient with manual reconstruction using ImageJ reached 0.88–0.98, reducing reconstruction time from several hours to seconds, achieving efficient and accurate automated reconstruction.

This invention provides a robotic quantitative perforation membrane disruption method, belonging to the field of micromanipulation, comprising the following steps: S1, establishing a perforation patch-clamp cellular circuit model based on cell electrophysiological characteristics; S2, deriving the relationship between the measured resistance and the number of micropores during perforation using the above model; S3, deriving the relationship between the transmembrane current under zero-state response and the measured resistance by applying periodic step voltage signals to the cells; S4, based on this modeling result, combining a robotic perforation patch-clamp system, using the peak value of the transmembrane current under zero-state response to input a step voltage for real-time feedback on the number of micropores, thereby achieving closed-loop control of the release of perforated material and realizing robotic quantitative perforation membrane disruption. This invention overcomes the problem of difficulty in quantifying the perforation degree of current perforation patch-clamp methods by deriving a perforation patch-clamp cellular circuit model, achieving a quantitative perforation membrane disruption success rate of 90%, and an average perforation speed nearly twice that of ordinary perforation membrane disruption methods.

The application discloses arylpiperazine salt compounds, a synthesis method thereof and application thereof in antidepressants, and belongs to the field of medicinal chemistry. The arylpiperazine salt compounds containing bioactivity are obtained through a plurality of continuous reactions such as N-alkylation, protection of a group, condensation and deprotection, etc. of arylpiperazine as raw material. Researches show that the compounds exhibit high receptor binding of 5-HT 2A R and GABA A Rs. The target points are confirmed through molecular docking, micro-thermal mobility instrument, surface plasmon resonance, inositol-phosphoric acid (IP1) accumulation and cell membrane patch clamp, etc.; and the anti-inflammatory activity of the compounds is evaluated by enzyme-linked immunosorbent assay, so as to provide a potential active structure skeleton for development of antidepressants.

The present application relates to the application of isoquercitrin in the preparation of a drug for treating post-traumatic stress disorder, and belongs to the technical field of natural chemical medicine development. The present application systematically clarifies the whole-chain mechanism of isoquercitrin in treating PTSD through the "GABA A R-PINK1 / Parkin-mitophagy" pathway by means of a variety of technical means such as molecular docking, microthermal swimming, patch clamp, proteomics, cell and animal models. Compared with the clinically commonly used drugs for treating post-traumatic stress disorder, isoquercitrin, as a natural flavonoid compound, can improve the symptoms of PTSD while not damaging the learning and memory function, and has better safety. Moreover, isoquercitrin is widely sourced and has a mature preparation process. The present application provides a theoretical basis and experimental support for the application of isoquercitrin in the development of drugs for treating post-traumatic stress disorder, and has important clinical application value and industrialization prospects.

This invention discloses a method for detecting viral or pseudoviral cell infection, comprising: applying a sinusoidal voltage to an attached membrane in patch-clamp mode and detecting the corresponding membrane current; inducing viral or pseudoviral cell infection by binding of viruses or pseudoviruses in the electrode fluid to receptors on the attached membrane; performing phase discrimination demodulation on the corresponding membrane current signal using the phase of the input sinusoidal voltage as a standard to obtain the imaginary and real parts of the membrane current and calibrating and standardizing them in real time; calculating the membrane capacitance of the attached membrane based on the real and imaginary parts of the current signal, based on the calibration and standardization; identifying viral or pseudoviral cell infection events and their modes based on the dynamic characteristics and polarity of the membrane capacitance changes; and estimating the size of the virus or pseudovirus based on the magnitude of the membrane capacitance changes. This invention enables real-time, high-precision detection of various viruses or pseudoviruses infecting host cells via envelope-cell membrane fusion or endocytosis in acutely isolated cells, cultured cells, and isolated tissue cells.

The present disclosure relates to methods, apparatuses, and systems to measure one or more electrical signals from a cultured cell layer. In some embodiments, such electrical signals can be indicative of transport or lack of transport of an agent through the cell layer.

The embodiment of the invention provides a multi-channel temperature control device of a patch clamp system, and relates to the technical field of patch clamp equipment, the multi-channel temperature control device comprises a base, a mounting table and an operating table, the mounting table and the operating table are arranged on the base, a test tube, an injection and a material tube are arranged on the mounting table, and a test vessel for bearing living cells is mounted on the operating table; a storage groove vertically penetrating through the operation table is formed in the operation table, the test vessel is located in the storage groove, two heating cylinders are rotationally connected to the base, a heat conduction belt is arranged on the heating cylinders in a surrounding mode, and the heat conduction belt is located under the test vessel. A servo motor for driving the heating cylinder to rotate is fixed to the base, a heating device for heating the heating cylinder is arranged in the base, a plurality of heat conduction wires surrounding the heat conduction belt are arranged on the heat conduction belt, and a plurality of first temperature sensors are arranged on the base. The first temperature sensors are distributed in the movement direction of the heat conduction belt. The device has the advantages that living cells can be conveniently heated, and the situation that the living cells die due to the fact that the temperature change is too large is prevented.