367 results about "Nerve cells" patented technology

The invention discloses a compound probiotic for improving cognitive function of old people and a preparation method and application thereof. Firstly, maltodextrin is dried and then sieved, metal impurities are removed after sieving, and maltodextrin freeze-dried powder, lactobacillus paracasei Lpc37 freeze-dried powder, phytobacterium plantarum Lp-115 freeze-dried powder and bifidobacterium animalis subsp. Lactis Bl-04 freeze-dried powder are weighed and sieved respectively; the preparation method comprises the following steps: preparing freeze-dried powder of three strains, then premixing the freeze-dried powder of the three strains with a part of maltodextrin, adding the residual maltodextrin after the strains are premixed, continuously mixing until the materials are uniformly distributed to obtain the compound probiotics, exerting a synergistic effect through a specific strain combination (Lp115 + Lpc37 + Bl-04), providing a new way for non-drug intervention, being beneficial to regulating intestinal flora metabolism (such as generating short-chain fatty acid SCFA), and improving the immunity of the human body. The synthesis and release of neurotrophic factors are promoted, the nerve cell function is improved, and the cognitive function improvement is indirectly assisted.

The invention belongs to the technical field of drug development, and particularly relates to application of 2-fluoro-N-(2-(4-methyl-2-(m-tolyl) thiazole-5-yl) ethyl) benzenesulfonamide in preparation of drugs for treating nervous system diseases. The invention aims to provide a medicine which has small toxic and side effects and can target SLC11A2 to treat or improve nerve cell injury. According to the technical scheme, the invention relates to application of 2-fluoro-N-(2-(4-methyl-2-(m-tolyl) thiazole-5-yl) ethyl) benzenesulfonamide in preparation of a medicine for treating nervous system diseases. It is proved that the compound 2-fluoro-N-(2-(4-methyl-2-(m-tolyl) thiazole-5-yl) ethyl) benzenesulfonamide is small in toxic and side effect, and nerve cell damage can be protected or improved by targeting SLC11A2; and a new choice is provided for treating or improving nerve cell injury.

The invention relates to the technical field of biological medicines, in particular to Hangzhou white chrysanthemum-derived exosome-like nano-vesicles as well as a preparation method and application thereof. Chrysanthemum morifolium ramat nano-vesicles are prepared from compositae plants through a high-speed centrifugation method, the average particle size of the nano-vesicles is 68 nm, and the Zeta potential is-19.50 + / -1.10 mV. The nano vesicles can effectively protect nerve cells and improve anxiety behaviors and cognitive impairment of SAE mice. The compound has a good application prospect in preparation of medicines for treating neuroinflammation and SAE.

The invention relates to the technical field of related drugs for nerve injury caused by heavy metal pollution, in particular to application of a compound for inhibiting phosphorylation of ARMC10 in preparation of drugs for antagonizing tin-induced nervous system injury. Exposure of trimethyltin chloride induces nerve cell mitochondrial dysfunction, and the key mechanism is ARMC10 serine 43 site abnormal phosphorylation. Phosphorylated protein causes excessive mitochondrial fission, membrane potential collapse and energy metabolism disorder, resulting in neuron damage. Based on the target spot, a compound for antagonizing tin-induced nervous system injury is obtained through screening, protein phosphorylation can be specifically inhibited, mitochondrial dynamic unbalance and dysfunction induced by trimethyltin chloride are effectively reversed, and neurotoxicity is relieved. According to the technical scheme, the technical problem that in the prior art, no medicine for effectively antagonizing tin-induced nervous system injury exists can be solved, and the compound has the application potential for treating the nervous system injury and cognitive impairment caused by tin exposure.

The invention belongs to the technical field of drug development, and particularly relates to application of (E)-N-benzyl-2-((5-(thiophenyl) furan-2-yl) methylene) hydrazine-1-thioformamide in preparation of drugs for treating nervous system diseases. The invention aims to provide a medicine which has small toxic and side effects and can target SLC11A2 to treat or improve nerve cell injury. According to the technical scheme, the invention relates to application of (E)-N-benzyl-2-((5-(thiophenyl) furan-2-yl) methylene) hydrazine-1-thioformamide in preparation of a medicine for treating nervous system diseases, in particular to application of (E)-N-benzyl-2-((5-(thiophenyl) furan-2-yl) methylene) hydrazine-1-thioformamide. It is proved that the compound (E)-N-benzyl-2-((5-(thiophenyl) furan-2-yl) methylene) hydrazine-1-thioformamide is small in toxic and side effect, and nerve cell damage can be protected or improved by targeting SLC11A2; and a new choice is provided for treating or improving nerve cell injury.

The invention relates to the technical field of coal-fired unit energy flow analysis and prediction, in particular to a coal-fired unit boiler energy flow analysis and prediction method under rapid variable load, which specifically comprises the following steps of: firstly, combining a thermodynamic physical framework with a recurrent neural network, and representing a function relationship between a time-varying parameter and a state variable in a mechanism model through a neural unit; rapid fluctuation of energy in the boiler is accurately captured; secondly, a multi-mode switching strategy based on an attention mechanism is introduced, flexible switching is carried out between a strong coupling state and a weak coupling state, and the prediction precision under high-speed load change is guaranteed; and finally, introducing an error compensation model, and dynamically correcting a hybrid model prediction residual error. According to the method, physical constraints of mechanism modeling, multi-mode switching of an attention mechanism and an error compensation model are integrated and fused, so that the problems that most of hybrid modeling studies in the prior art mainly focus on steady-state or finite transient prediction, and studies on energy flow dynamic characteristics under rapid load change are still relatively deficient are solved.

A Bifidobacterium animalis subsp. lactis for reducing Abeta42 deposition and an application, a name of the Bifidobacterium animalis subsp. lactis is Bifidobacterium animalis subsp. lactis IOB-LO7, and classified as Bifidobacterium animalis subsp. lactis, the Bifidobacterium animalis subsp. lactis IOB-LO7 is preserved in the General Microbiology Center of the China General Microbiological Culture Collection Center (CGMCC) on Dec. 23, 2021, with the collection number of CGMCC No. 24185. By reducing the levels of Aβ42 in the cerebral cortex and hippocampus, clearing Aβ amyloid plaques, improving communication between neurons, reducing brain neuroinflammation, and protecting nerve cells, it helps to restore cognitive function and improve Alzheimer's disease. Additionally, it can also improve gut microbiota imbalance caused by Alzheimer's disease.

The invention discloses a fusion polypeptide TGNL and application of neural stem cells modified by the fusion polypeptide TGNL in spinal cord injury repair. The fusion polypeptide TGNL is composed of a TAT penetrating domain, a GSTP targeting domain, an NICDi inhibition domain, an MMP-2 activation domain and a flexible Linker connecting the three domains, and the full-length amino acid sequence is as shown in SEQ ID NO. 1. The invention also provides a preparation method of the polypeptide, a neural stem cell (hNSCs-TGNL) for expressing the polypeptide and a construction method of the neural stem cell (hNSCs-TGNL). Experiments show that the fusion polypeptide TGNL can efficiently penetrate nerve cells, specifically target glial scars, is selectively activated by MMP-2, and promotes neural stem cells to differentiate into neurons by inhibiting Notch signals; the hNSCs-TGNL has high survival rate and strong penetrability in an in-vitro glial scar model, and can significantly promote motor function recovery, reduce injury volume, increase axon density and reduce glial scars in a rat spinal cord contusion model. The invention provides a novel candidate drug and a cell treatment strategy for clinical treatment of spinal cord injury, and has remarkable clinical application value.

The invention discloses a nasal delivery multistage targeting bionic nano drug delivery system as well as a preparation method and application of the nasal delivery multistage targeting bionic nano drug delivery system. According to the drug delivery system, a black phosphorus nanosheet with an active oxygen scavenging effect is used as an inner core carrier, a neuroprotective agent fingolimod hydrochloride FTY720 and polydimethyldiguanide PolyMet are loaded, and a microcolloid cell membrane and a mitochondrial membrane are modified on the surface of the preparation; the nano drug delivery system is applied to treatment of cerebral arterial thrombosis reperfusion injury, can overcome the blood brain barrier, can quickly enter the brain through nasal administration, realizes efficient delivery of drugs, remarkably reduces the cerebral infarction area, reduces the brain inflammation level, protects nerve cells, greatly relieves cerebral arterial thrombosis reperfusion injury, and has application prospects.

The present invention relates to a helmet-type magnetic stimulation device for providing non-invasive treatment by applying a magnetic field to cranial nerve cells or scalp cells, the helmet-type magnetic stimulation device comprising: a helmet-type case part; a magnetic field generation part for generating a magnetic field in order to apply a magnetic field to the head; and a fixing part installed on one inner side of the case part to fix and support the magnetic field generation part at a predetermined position on the head. Therefore, the present invention includes a magnetic field generation part for generating a magnetic field in order to apply a magnetic field to the head, inside the case part.

The invention provides isolated Ackermansiella muciniphila, a composition containing the same and an anti-aging and / or anti-oxidation application of the Ackermansiella muciniphila. The Akkermansia muciniphila has the advantages that the average life and the longest life of the Akkermansia muciniphila in a nematode model can be prolonged, the movement activity, the swallowing function and the reproductive capacity of the Akkermansia muciniphila can be improved, the expression of insulin / IGF-1 pathway genes (daf-16, daf-2 and age-1), the expression of antioxidant genes (gst-4 and sod-3) and the expression of longevity related genes (sir-2.1) of the Akkermansia muciniphila can be up-regulated, and the antioxidant capacity and the heat stress tolerance of the Akkermansia muciniphila can be enhanced. It is shown that the Akkermansia muciniphila provided by the invention can be used for preparing or developing the anti-oxidation and anti-aging products, and the anti-oxidation and anti-aging products can be prepared from the Akkermansia muciniphila. The invention provides an efficient and safe solution for anti-aging and / or anti-oxidation.

The invention discloses a neuroprotective peptide with anti-inflammatory and anti-oxidation effects and application of the neuroprotective peptide. The neuroprotective polypeptide is a polypeptide with any one of the following sequences: FPDFVYK and APDTRLF. The invention provides an antioxidant effect of the neuroprotective polypeptide in a PC12 cell injury model induced by hydrogen peroxide, and the neuroprotective polypeptide can be used for preparing antioxidant drugs. The invention provides an anti-inflammatory effect of the neuroprotective polypeptide in a PC12 cell injury model induced by hydrogen peroxide, and the neuroprotective polypeptide can be used for preparing anti-inflammatory drugs. The invention provides the influence of the neuroprotective polypeptide on the expression of pathways and neurotrophic factors in PC12 nerve cells. The neuroprotective polypeptide reduces the activity of acetylcholin esterase (AChE) and up-regulates the expression of neurotrophic factors so as to alleviate nerve cell damage and enhance memory, thereby achieving the purpose of preventing and treating neurodegenerative diseases.

The invention discloses a ketamine derivative and application thereof in treatment of mental diseases, and belongs to the technical field of medicines. The compound or the pharmaceutical composition thereof provided by the invention can obviously improve the expression quantity of nerve cells BDNF, promote the growth of neurite, inhibit NMDA receptor signals and treat acute and chronic depression, has improved oral bioavailability compared with ketamine, and can be used for treating mental diseases such as depression.

A method of promoting neuroprotection in a subject from axonal degeneration, neuronal cell death, and / or glia cell damage after injury, augmenting neuronal signaling underlying learning and memory, stimulating neuronal regeneration after injury, and / or treating a disease, disorder, and / or condition of the nervous system in a subject in need thereof includes administering to the subject a therapeutically effective amount of a 15-PGDH inhibitor.

The present invention provides a kind of composition containing bird's nest acid, phospholipid and polyunsaturated fatty acid and preparation method thereof, composition, when not adding any additives, bird's nest acid is dispersed in DHA grease in functional ingredients, while solving the problem that DHA is easily oxidized, promotes brain development, regulates immunity, promotes the absorption and utilization of minerals in the intestine and the effects of beauty and skin care. Phospholipids can prevent the sedimentation of bird's nest acid in ω 3 polyunsaturated fatty acid grease, and are phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol or phosphatidylserine. Not only can the sedimentation of bird's nest acid be prevented, but also the growth of nerve cells can be supported, thereby improving the absorption rate of DHA in the brain. Especially PS, itself can not only nourish the brain, enhance brain function, but also relieve mental stress and improve cognition, and is called "brain nutrient".

The application belongs to the field of medical biological materials, and relates to a preparation method of a hierarchical nanofiber composite film for nerve repair and application thereof. The method comprises the following steps: preparing multilayer nanofibers with different internal stresses through continuous multiple electrostatic spinning units, stacking the multilayer nanofibers with different internal stresses to form a composite nanofiber film with an internal stress gradient; chemically crosslinking the composite nanofiber film; performing adhesion molecule adhesion treatment on the upper surface of the composite nanofiber film; and preparing the hierarchical nanofiber composite film with an internal stress gradient and capable of self-deforming under the action of liquid to wrap nerves. The film provided by the application has excellent flexibility, biocompatibility and degradation performance, can efficiently and quickly adhere to the surface of damaged nerves, provides a good support microenvironment for nerve cell growth, promotes nerve regeneration and repair, and has a wide application prospect in the field of peripheral nerve injury repair.

The present application relates to a pharmaceutical composition comprising an NRF2 agonist and borneol, both of which synergize in a specific molar ratio range, enhance the overall anti-inflammatory and antioxidant biological activity of the drug, improve the oral bioavailability and target tissue distribution of the drug, inhibit the off-target side effects of the NRF2 agonist, and significantly improve the effectiveness and safety of the drug.

The invention discloses a brain nerve cell targeted gene protein co-loaded nano delivery system and a preparation method thereof, phenylboronic acid modified polyethyleneimine is synthesized through substitution reaction, then the polyethyleneimine is compounded with DNA through electrostatic interaction to form a PEI-PBA / DNA nano compound, bovine serum albumin is loaded through nitrogen-boron complexation, and the brain nerve cell targeted gene protein co-loaded nano delivery system is obtained. The preparation method comprises the following steps: preparing a gene-protein co-loaded nano delivery system PPDB, further endowing the nano delivery system with a function of crossing a blood brain barrier, and introducing glycerophosphorylcholine through the formation of a borate bond, so as to prepare the cerebral nerve cell targeted gene-protein co-loaded nano system PPDBG. The preparation method disclosed by the invention is simple, realizes simultaneous loading of genes and proteins with completely different physical structures, and has very important research significance on brain-related diseases such as epilepsy and the like. Meanwhile, the introduction of the boric acid ester bond endows the nano delivery system with the on-demand plug-and-play characteristic, so that the spectral applicability of the nano delivery system is realized.

The application discloses an active polypeptide and application thereof in treating diabetic peripheral neuropathy, and belongs to the technical field of biological polypeptide drugs. An amino acid sequence of the active polypeptide is QVWPDGFVGLAKLWI, which is obtained by step-by-step enzymatic extraction and purification from Morus alba L. which is a homology of medicine and food. The polypeptide can significantly inhibit oxidative stress, inflammatory infiltration and cell apoptosis of Schwann cells induced by a high-sugar environment, and repair the function of damaged nerve cells. Meanwhile, the polypeptide can effectively improve abnormal sciatic nerve conduction function of a diabetic model mouse. The active polypeptide can be used for preparing a drug for treating diabetic peripheral neuropathy, and provides a new candidate drug and research and development direction for the targeted treatment of diabetic peripheral neuropathy.

The application provides a toxicity-enhanced neurotoxin recombinant protein and application thereof, and belongs to the technical field of biological medicine. The recombinant protein comprises a botulinum toxin type A receptor binding domain and at least one GM1 binding peptide inserted into the botulinum toxin type A receptor binding domain, and the recombinin protein can recognize and bind to ganglioside GM1. The short peptide sequence capable of binding to ganglioside GM1 is introduced into the botulinum toxin type A receptor binding domain to obtain the recombinant protein, the binding capacity of the recombinant protein to ganglioside GM1 is enhanced, the target recognition and binding capacity of the recombinant protein to the nerve cell membrane are improved, the overall affinity and endocytosis efficiency of the recombinant protein to the nerve cell are improved, and the neurotoxicity of the botulinum toxin is enhanced. The application not only improves the binding efficiency of BoNT / A in the in-vitro nerve cell model, but also shows a higher toxicity level in the functional verification, and shows a good clinical conversion prospect.

The application provides a method for regulating nerve cells based on photo-induced micro-nanometer oscillators and application thereof, and belongs to the technical field of nerve cell regulation. The method comprises the following steps: adding photo-induced micro-nanometer oscillators in an extracellular system of nerve cells, and constructing a first optical potential well by using an optical tweezer system; the first optical potential well captures the photo-induced micro-nanometer oscillators, and positions the photo-induced micro-nanometer oscillators beside the nerve cells; the optical tweezer system is used to periodically switch the laser beam between two preset positions, so that the photo-induced micro-nanometer oscillators vibrate. The application utilizes optical force to drive the photo-induced micro-nanometer oscillators to vibrate and generate local ultrasonic waves, and does not need gene modification or implantation of devices, and the spatial resolution can reach a sub-micron level, thereby solving the contradiction between precision, damage and applicability in the prior art, and providing a new tool for nerve regulation.

The invention belongs to the technical field of molecular biology, and relates to an application of transferrin (TF) as an internal reference protein in preparation of a reagent for detecting exosome proteins of neurodegenerative diseases. The TF can maintain a stable expression level in nerve cells, brain tissues, blood plasma and serum-derived exosomes of a subject suffering from neurodegenerative diseases, including Alzheimer's disease, mild cognitive impairment, amyotrophic lateral sclerosis, Parkinson's disease or Huntington's disease, and also including neurodegenerative diseases such as Alzheimer's disease, mild cognitive impairment, amyotrophic lateral sclerosis, Parkinson's disease or Huntington's disease. The TF expression variation coefficient is obviously lower than that of a common exosome marker, and the expression level of TF has stronger correlation with the total protein amount of the exosome and is not influenced by external factors such as cell inflammation stress. The TF as the internal reference protein has more excellent performance, can help to more accurately reflect the total loading amount of the sample, and provides a more stable and reliable standardized tool for the field of exosome research.

The invention discloses application of a negative hydrogen ion combined stem cell preparation in preparation of antidepressant drugs, and belongs to the technical field of biological medicines. The negative hydrogen ion combined stem cell preparation is used for preparing the anti-depression medicine for treating depression-like rats, pleasant sensation deficiency and despair-like behaviors of the depression-like rats can be remarkably improved, nerve cell energy supply is improved, oxidative stress injury is strongly inhibited, and the curative effect of the anti-depression medicine is remarkably superior to that of single negative hydrogen ion treatment or single stem cell treatment. Through the synergistic effect of negative hydrogen ion anti-oxidative stress combined with stem cell preparation neural restoration, the oxidative stress-nerve injury core pathological pathway of depression is specifically blocked, a new scheme with better effect and more comprehensive mechanism is provided for depression treatment, and the pharmaceutical composition has important clinical transformation value.

The present application relates to a kind of water gel support prepared by self-assembled polypeptide and the use of support obtained therefrom, the preparation method includes: 1) self-assembled peptide sequence is linked nerve growth factor analog peptide by covalent bond to obtain bonded functional polypeptide;2) macrophage is separated and cultured, and is induced to be " alternative activation " anti-inflammatory M2 macrophage, obtain culture supernatant and carry out filtration, obtain filtered cell culture supernatant;3) filtered cell culture supernatant is mixed with bonded functional polypeptide, obtain mixed solution and adjust the concentration of the mixed solution, the bonded functional polypeptide self-assembles and forms water gel support.The present application uses M2 macrophage condition culture supernatant to construct regenerative microenvironment, combines polypeptide functional water gel support, realizes better overall interaction, and prepares the water gel support that can supplement and regulate nerve regeneration, the support can promote nerve cell regeneration behavior, provides new selection for tissue engineering biomaterials.

This invention relates to a juglansin and lipoic acid conjugate, its preparation method, and its applications. The juglansin and lipoic acid conjugate is named CL-1. CL-1 exhibits significant protective effects in models of oxidative and inflammatory damage in nerve cells, with a significantly greater reduction in TNF-α and IL-6 levels than the monomeric juglansin and lipoic acid, indicating that CL-1 has superior anti-inflammatory and anti-neural damage effects compared to the monomeric drugs, and possesses the potential to treat neurodegenerative diseases such as Alzheimer's disease. The provided preparation method is designed specifically for the structural characteristics of juglansin and lipoic acid, enabling efficient and large-scale synthesis of CL-1.

Disclosed in the present invention are a peripheral nerve catheter and a preparation method therefor. The peripheral nerve catheter comprises: an outer sheath, which is a hollow braided tube that has micron-sized pores and is non-wound; at least one inner core tube, which is placed in the outer sheath and is coaxially arranged with the outer sheath, each inner core tube being a hollow fiber tube that has micro / nano-sized pores and is non-wound; and filling gel, which is filled between the outer sheath and the inner core tube to fix the inner core tube. The bionic nerve catheter having a structure composed of the inner core and the outer sheath according to the present invention can achieve effective nerve regeneration, the inner core tube, which axially runs through the outer sheath, has a topological structure that is conducive to the adhesion and directional growth of nerve cells, and also has a micro / nano-sized pore structure that is conducive to the penetration of nutrients for growth and the formation of nerve connections and neurovascular network. In addition, the filling and curing of the filling gel effectively prevents the infection of damaged tissues, and is conducive to the penetration of nutrients and the transfer of cellular metabolic waste.

The present application relates to the compound Isarubrolone C and its application, specifically, the structure of Isarubrolone C is as shown in the following formula, the present application also relates to the application of the compound Isarubrolone C in the preparation of the drug for preventing and / or treating the liver cell or nerve cell damage caused by nitration stress.

The application belongs to the field of food processing and biomedical technology, and discloses a preparation method of a salty taste enhancing peptide-naringenin compound and application of the compound in improving cognitive decline. The compound is prepared by compounding salty taste enhancing peptides from soft-shelled turtle eggs and naringenin at a ratio of 20-150:1 (w:w). The preparation process comprises the following steps: soft-shelled turtle egg enzymolysis, ultrafiltration, freeze-drying to obtain salty taste enhancing peptides, mixing the salty taste enhancing peptides with a naringenin solution, concentration, and freeze-drying. The compound has good stability, salty taste enhancing and antioxidant activities, can reduce the use of salt and maintain salty taste. The compound can improve cognitive decline and anxiety caused by aging and diabetes, improve autonomous activity, memory and social skills, strengthen physical fitness, protect nerve cells and skeletal muscles, maintain brain tissue health, and can be applied to the preparation of related preparations for improving cognitive decline.

Disclosed herein are compounds and methods of use thereof effective for the treatment of neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS), a disease that affects nerve cells in the brain and spinal cord, eventually causing loss of muscle strength. The compounds of the disclosure include, cutamesine, a synthetic sigma receptor agonist selective for the 81 receptor, and also a chaperone protein of the central nervous system that plays a key role in the modulation of calcium ions and apoptosis, as well as a sapogenin, such as smilagenin, a non-peptide neurotrophic factor that aids in the reversal of free radical neurotoxicity.

The application of CpG oligonucleotides in drugs for diabetic cardiac autonomic neuropathy: Experiments have confirmed that CpG oligonucleotide 1826 can reduce the activation of satellite glial cells in the stellate ganglion and the expression of inflammatory cytokines, inhibit the expression of purine 2Y12 receptors, and reduce lipid peroxidation damage to alleviate ferroptosis in stellate ganglion neurons, thereby improving cardiac autonomic nerve function. Examples of CpG oligonucleotide 1826 demonstrate that CPG-ODN with anti-inflammatory effects or with anti-damage protective effects on the sympathetic nervous system has preventive and therapeutic effects in diabetic cardiac autonomic neuropathy and sympathetic nerve injury-related diseases.