10 results about "Nerve graft" patented technology

The present invention provides an automated method for analyzing and quantifying chondroitin sulfate proteoglycan (CSPG) depletion in processed nerve graft tissue samples.

The application discloses a preparation method of a decellularized tissue nerve graft scaffold with electric conductivity, and belongs to the field of biomaterials. The method comprises the following steps: separating embryonic period umbilical cord tissue, removing cells and lipids, and then performing freeze-drying; a titanium carbide monolayer nanosheet solution is prepared by using a chemical etching method, a GelMA solution is prepared by using a photo initiator phosphate buffer salt solution, and the GelMA solution is mixed with the titanium carbide monolayer nanosheet solution to obtain a conductive hydrogel; the embryonic period umbilical cord tissue is wrapped with the conductive hydrogel, and is cured by ultraviolet light to obtain the decellularized tissue nerve graft scaffold with electric conductivity. Compared with the prior art, the nerve graft scaffold has good electric conductivity, mechanical properties and degradability.

The application belongs to the technical field of nerve grafts, and discloses a multilayer composite capacitive film, a preparation method and application of the multilayer composite capacitive film in nerve repair scaffolds. The scaffold is composed of three layers, namely, an A layer, a B layer and an A layer. The A layer is a sandwiched layer on both sides, and comprises a mixture of chitosan (or silk fibroin or a decellularized matrix) and a conductive polymer PEDOT:PSS and a crosslinking agent polyethylene glycol diglycidyl ether (PEGDE). The B layer is a middle hydrogel sandwiched layer, and comprises methacrylated gelatin (Gel-MA). The A layers on both sides are bonded through the B layer to form a viscoelastic hydrogel after ultraviolet light irradiation, and the composite capacitive scaffold in a multilayer structure with a through middle cavity is obtained after curing and air drying. The formed scaffold has good electrical conductivity, mechanical properties and biological safety, and can effectively induce the proliferation, migration and differentiation of RSC96 Schwann cells and nerve system cells PC12, thereby providing a good physical stable support and a favorable microenvironment for peripheral nerve injury repair.

Systems, devices, and methods for treating a nerve injury in a patient are provided. Systems include a nerve cap graft including tissue harvested from a tissue source. Methods include deploying a nerve cap graft at an implant site in a patient to provide a therapeutic benefit. The devices include a matrix designed to fit on one or more nerve stumps of a patient and the devices are designed to provide a patient with a therapeutic effect.

The invention provides a nerve conduit for nerve injury repair and a preparation method thereof, and belongs to the technical field of medical materials. The nerve conduit provided by the invention is composed of three-dimensional conductive composite hydrogel loaded with human endometrial mesenchymal stem cells, and conductive polymer polypyrrole is grafted and crosslinked in a natural biological material network formed by chitosan and gelatin. The catheter has good biocompatibility, degradability and stable conductivity, can simulate an electrophysiological microenvironment for nerve regeneration, and can generate synergy with a paracrine function of stem cells, so that the directional regeneration of axons is effectively guided, and the formation of myelin sheaths is promoted. In-vivo and in-vitro experiments show that when the catheter is used for repairing peripheral nerve long segment defects, the function recovery effect of the catheter is equivalent to that of autologous nerve transplantation, and an ideal alternative scheme is provided for clinic.

The application discloses a nerve graft, a preparation method and application, and relates to a nerve conduit with an inner wall loaded with activin A-containing microspheres. The nerve graft provided by the application is a growth factor delivery system with excellent performance and controllable release kinetics, can realize efficient repair of a nerve defect of a rat, and the preparation method of the nerve graft is convenient and easy to operate, thereby laying a reliable technical foundation for development of a next-generation intelligent nerve graft.

A method of preparing a nerve graft includes submerging a nerve graft in a solution including FK506 and a solvent to promote incorporation of FK506 into the nerve graft. A tissue graft includes nerve tissue and FK506 incorporated within the nerve tissue. In the tissue graft, the FK506 is free of hydrogel and not encapsulated.

A method of preparing a nerve graft includes submerging a nerve graft in a solution including FK506 and a solvent to promote incorporation of FK506 into the nerve graft. A tissue graft includes nerve tissue and FK506 incorporated within the nerve tissue. In the tissue graft, the FK506 is free of hydrogel and not encapsulated.

The invention provides a magnetic-thermal double-control dynamic energy supply nano-motor nerve graft and a preparation method thereof, the nerve graft is composed of a degradable polymer fiber catheter, temperature-sensitive hydrogel filled in the nerve graft and a hydrogel-loaded nano-motor, and succinic acid and other energy molecules are loaded on the surface of the nano-motor. The nano-motor is prepared from a high magnetic response material, directional guidance is realized under the action of an external magnetic field, and heat generated under an alternating magnetic field can trigger the temperature-sensitive hydrogel to generate gel-sol conversion; energy molecules can be continuously released to improve local ATP level, maintain cell homeostasis and support axon regeneration and synaptic remodeling. The advantages of magnetic drive directional navigation and temperature-sensitive controlled release are fused, the defects that a traditional scheme is insufficient in orientation, lack of energy and the like are overcome, the graft is good in biocompatibility and biodegradable, the immune microenvironment of the injured nerve can be regulated and controlled, oxidative stress is avoided, and regeneration and functional recovery of the long-segment injured peripheral nerve are efficiently promoted.