84 results about "Decellularized matrix" patented technology

The invention discloses a hydrogel-acellular matrix composite patch containing functionalized extracellular vesicles and a preparation method of the hydrogel-acellular matrix composite patch. The composite patch comprises a hydrogel layer and an acellular pericardium matrix material layer, and an extracellular vesicle-macrophage membrane fusion body (EV-MM) is entrapped in the cross-linked hydrogel. From two aspects of improving the targeting of EVs to the cardiac infarction part and enhancing the retention of the EVs at the cardiac infarction part, the distribution condition of the EVs at the cardiac infarction part is improved, so that the action efficiency of the EVs is improved, and the treatment effect is improved.

The invention discloses a preparation method of an acellular matrix containing a bioactive coating, and belongs to the field of medical beauty industry, the key point of the technical scheme is that the preparation method comprises the preparation steps of S1, preparation of the acellular matrix; s2, treating the acellular matrix; s3, rehydrating the decellularized matrix, sequentially immersing the rehydrated decellularized matrix into an activating solution, a collagen solution and an activating solution, reacting, washing the collagen matrix, and freeze-drying to obtain the collagen matrix; s4, adding the anti-inflammatory microcapsules and the growth factor nano-carrier into the hyaluronic acid solution to obtain a mixed solution; s5, the collagen matrix is immersed in an activating solution, after reaction and washing, the surface of the collagen matrix is coated with the mixed solution for reaction, after curing, washing and drying are conducted, and the acellular matrix containing the bioactive coating is obtained.The covalently-connected hyaluronic acid-collagen double-layer structure is constructed on the surface of the acellular matrix, so that biocompatibility and safety are improved; meanwhile, the problems that a traditional acellular matrix is unstable in structure and poor in mechanical strength are solved.

The invention discloses preparation and application of dual-network hydrogel. The dual-network hydrogel comprises the following components: (Z1) a spinal cord acellular matrix network DSM formed by self-assembly of a spinal cord acellular matrix dSECM; and (Z2) a metal-polyphenol network MPN formed by coordination of metal ions and polyphenols; wherein the DSM and the MPN are cross-linked through hydrogen bonds to jointly form a double-network structure. DSM provides a microenvironment suitable for cell survival and growth for the dual-network hydrogel and induces stem cells to differentiate into neurons, and MPN provides the dual-network hydrogel with biological effects of inhibiting inflammation and accumulation of reactive oxygen species (ROS). The dual-network hydrogel has the advantages of the two components, the active component neurotrophic factor and the inhibitor loaded on the dual-network hydrogel can regulate and control immune response after SCI, improve neuroinflammation microenvironment and promote nerve repair, and a new strategy is provided for in-situ tissue repair of SCI.

The application belongs to the technical field of medical materials, and discloses a soft tissue repair patch and a preparation method thereof, which comprises the following steps: S1, treating and cleaning animal soft tissue to obtain a first intermediate product; S2, adding the first intermediate product into a reagent to perform freeze-thaw circulation to obtain a second intermediate product; S3, soaking and treating the second intermediate product with an organic solvent and cleaning to obtain a third intermediate product; S4, soaking and treating the third intermediate product with an alkali solution and cleaning to obtain a fourth intermediate product; and S5, soaking and treating the fourth intermediate product with an acid solution, cleaning, drying and sterilizing to obtain the soft tissue repair patch. The application provides a more gentle and friendly decellularization scheme, and the decellularized matrix prepared by using the scheme is more completely decellularized, has no chemical residue, and maintains the natural scaffold structure of collagen.

Provided is a use of a biological material in preparing a product for limiting gastric volume. The biological material is selected from a decellularized matrix, an artificial extracellular matrix and a high molecule compound. The wrapping of a remaining stomach after gastric volume reduction surgery by the biological material can effectively reduce the expansion of the gastric volume after the surgery for a long time. The gastric volume of the remaining stomach can be effectively maintained, avoiding the occurrence of weight regain caused by the expansion of the remaining stomach. The decellularized matrix and a biological material remolded tissue have the viscoelasticity equivalent to or close to natural tissue, avoiding gastric wall stiffness, and thus not affecting gastric wall peristalsis.

By means of the method according to one aspect, in a process of enhancing biocompatibility of an acellular matrix by supercritical carbon dioxide treatment, immunogenicity of the acellular matrix can be reduced with effective efficiency while maintaining a content of a physiologically active substance and physiologically active ability. Accordingly, the acellular matrix prepared by the method according to one aspect, the composition including the same, and the biomaterial have low biotoxicity and excellent tissue regeneration energy, and thus can be usefully used in cosmetic or medical materials.

The invention provides a tissue engineering heart valve stent with a sulfonated nano-enzyme coating as well as a preparation method and application of the tissue engineering heart valve stent, and belongs to the technical field of medical materials. The invention relates to a tissue engineering heart valve stent with a sulfonated nano-enzyme coating, which comprises an acellular matrix carrier, ruthenium / iridium-tannic acid nano-enzyme loaded on the acellular matrix carrier, and a sulfonated polymer coating fixed on the acellular matrix carrier and / or the surface of nano-particles through ultraviolet polymerization, the ruthenium / iridium-tannic acid nano-enzyme is formed by tannic acid TA, Ru < 3 + > and Ir < 3 + > through coordination. According to the invention, through double bionic activities of superoxide dismutase and nitric oxide synthase of ruthenium / iridium-tannic acid nano-enzyme and through the super-hydrophilicity and electrically neutral surface of the sulfonated polymer coating, collaborative regulation and control of O2-NO cascade catalysis and interface functions in a pathological microenvironment are realized; the structural and functional in-situ regeneration of the tissue engineering heart valve stent is promoted.

The invention provides acellular matrix hydrogel loaded with animal acellular omentum particles as well as a preparation method and application of the acellular matrix hydrogel loaded with the animal acellular omentum particles, and relates to the technical field of tissue engineering and regenerative medicine. Comprising a decellularization treatment agent, a decellularization matrix, functional particles, an enzymolysis agent, a cross-linking stabilizer, a functional additive and a solvent, the functional additive is prepared from PDA coated Fe3O4 magnetic nano particles, phosphorylated polyglycerol sebacate and liposome coated silver nano particles. The PDA-Fe3O4 magnetic nanoparticles of the functional additive generate mechanical signals under the action of an external magnetic field, a Piezo1 mechanical gating cation channel is activated to promote calcium ion internal flow, and the autonomous mineralization ability of phosphorylated polyglycerol sebacate is combined, so that the activity of the bone differentiation marker alkaline phosphatase is effectively improved, and the bone differentiation marker alkaline phosphatase is prepared. The combination of the PDA coated Fe3O4 magnetic nanoparticles and the phosphorylated polyglycerol sebacate integrates a magnetic response mineralization system.

The invention discloses an antibacterial composite periosteum for an oral cavity and a processing technology of the antibacterial composite periosteum, and relates to the technical field of periosteum. The method specifically comprises the following steps: step 1, treating defatted pig peritoneum with a decellularized solution, cleaning and sterilizing to obtain a pig peritoneum decellularized matrix; 2, treating the pig peritoneal decellularized matrix with a glyoxal solution, cleaning, and sterilizing to obtain a pretreated pig peritoneal decellularized matrix; step 3, uniformly coating the surface of the pretreated porcine peritoneal acellular matrix with an antibacterial solution, and performing sterilization, standing and freeze-drying to obtain the antibacterial composite periosteum. Wherein the antibacterial solution is prepared from the following raw material components in concentration: 1 to 2 weight percent of modified chitosan, 0.2 to 0.4 weight percent of amino-terminated polyethylene glycol and 0.01 to 0.05 weight percent of p-toluenesulfonic acid, and a solvent is a PBS (Phosphate Buffer Solution). The antibacterial composite periosteum is good in biocompatibility and has excellent clinical application value in oral surgery.

This invention relates to the field of biomedical materials technology, specifically to a decellularized matrix microparticle (dECM-MPs) 3D printing ink and its preparation method. The key technical points are: a heat-absorbing compound is temporarily dissolved on the surface of decellularized matrix microparticles (dECM-MPs) via recrystallization from a supersaturated solution; then, the microparticles are immersed in a temperature-sensitive gelatin solution. The heat absorbed during the dissolution of the heat-absorbing compound leads to a localized low temperature around the microspheres, causing the gelatin to solidify on the dECM-MPs surface, forming a physically cured gelatin hydrogel microlayer in situ. This microlayer is further stabilized by enzymatic cross-linking. Photosensitive modified hyaluronic acid molecules are introduced into the hydrogel microlayer to regulate its mechanical properties. Centrifugation yields a particulate ink suitable for extrusion 3D printing. After 3D printing, ultraviolet light irradiation is used to produce a 3D printed implant. This invention overcomes the problem that rigid dECM-MPs, such as decellularized cartilage microparticles or decellularized bone microparticles, cannot be extruded and printed. By regulating the mechanical properties of the hydrogel microlayer, particulate ink with excellent printing performance can be obtained. This method overcomes the limitation of rigid tissues being difficult to additively manufacture.

The application discloses a preparation method of a decellularized matrix and a product prepared by the method. The preparation method comprises the following steps: (1) washing a mammalian soft tissue to remove blood, lipids and impurities, and cutting the tissue into pieces with a thickness of 1-10 mm; (2) soaking the pieces in solution 1 for 6-48 h, and then soaking the pieces in solution 2 for 0.1-1 h; (3) treating the pieces in an ultrasonic water bath under the condition of a frequency of 20-40 kHz for 5-30 min; (4) water-bathing the pieces at 55-65 DEG C for 10-30 min; (5) sequentially washing the tissue with deionized water, a PBS buffer and ethanol, and washing the tissue with each solution for 1-3 times, each time for 10-30 min; and (6) freeze-drying the decellularized matrix material, and crushing the material at-80 DEG C to-20 DEG C to obtain a decellularized matrix powder. The preparation method significantly improves the cell component removal efficiency, effectively protects structural proteins such as collagen from being degraded in the freeze-drying and crushing processes, and ensures the integrity of the matrix in the aspects of morphology, structure and function, thereby providing a structural support and biological signal support for subsequent tissue repair.

The invention relates to the technical field of artificial dermis, in particular to a preparation method and application of artificial dermis based on bionic acellular matrix. The artificial dermis comprises a bionic acellular matrix component A and an auxiliary material component B, wherein the component A comprises type I collagen, type VI collagen, elastin, fibronectin, laminin, mother cell protein and glucan; the component B comprises antibacterial peptide, antibiotics and a high-molecular polymer material; the mass ratio of the component A to the component B is (4-6): (1-4). The preparation method comprises the following steps: respectively preparing procedure mother liquor of the component A and the component B, preparing hydrogel with a double-layer gradient structure through step-by-step crosslinking, and then carrying out freeze drying and sterilization treatment. The artificial dermis disclosed by the invention has good biocompatibility and antibacterial property, can effectively promote wound healing, is suitable for repairing acute and chronic wounds of four limbs, faces and trunk parts, and is particularly suitable for repairing complex wounds with exposed bones, tendons, nerves and blood vessels.

The application discloses an artificial blood vessel stent with anticoagulation performance and a construction method thereof. After a pig heart aorta blood vessel is subjected to decellularization treatment, a pre-gel solution is prepared by compounding polyvinyl alcohol and methacrylated gelatin, a designed model is printed into a hollow tubular mold by using 3D printing, the pre-gel solution is injected into the mold to obtain an artificial blood vessel stent with controllable shape and size after cross-linking, and finally, the artificial blood vessel stent loaded with heparin is obtained by loading the heparin on the prepared stent. The material for preparing the pre-gel solution is selected, and the advantages of the material functions can be complemented. The decellularized matrix is beneficial to cell adhesion, proliferation and differentiation; the compounding of polyvinyl alcohol and methacrylated gelatin improves the mechanical properties of the stent and improves the cell adhesion. The heparin is loaded on the artificial blood vessel stent to improve the anticoagulation performance of the stent and prevent the formation of thrombus. The stent serves as an excellent biomedical material and has a wide application prospect in the fields of cardiovascular tissue engineering and biology.

The application discloses a composite hydrogel based on urethral stem cell membrane sheet ECM and exosomes and a preparation method and application thereof, and relates to the technical field of tissue engineering, and in particular relates to a soluble ECM protein solution obtained by dissolving and enzymatically hydrolyzing urethral stem cell membrane sheet decellularized matrix (ECM) powder and mixing the soluble ECM protein solution with exosomes in a mass ratio of 3:1-10:1, and performing temperature-sensitive crosslinking in DMEM at pH 7.0 and 36-38 DEG C to form a hydrogel. The composite hydrogel based on urethral stem cell membrane sheet decellularized matrix (ECM) and exosomes integrates the cell activity of the urethral stem cell membrane sheet, the structural support of the decellularized matrix (ECM) and the signal regulation of the exosomes, constructs a three-level functional system, forms a synergistic repair effect, and is helpful to promoting angiogenesis in the urethra. Through covalent coupling of the decellularized matrix (ECM) fiber network and the surface protein of the exosomes, the intelligent release of the exosomes in an inflammatory environment is realized, and the composite hydrogel is used for urethral repair and angiogenesis promotion.

The present application belongs to the field of biomedical materials and tissue engineering, and particularly relates to a kind of acellular matrix aerogel repair material and its preparation method and application.Further, the present application relates to the comprehensive preparation method of the material, especially the extremely low immunogenicity acellular process combined with physical, chemical and supercritical fluid technologies, and the unique aerogel forming technology based on supercritical drying.Further, the present application relates to the application of the material, mainly related to soft tissue repair, medical cosmetology, bone / cartilage injury repair field.

The invention discloses application of an adipose acellular matrix prepared on the basis of adipose tissue in the aspect of drug sustained release. The fat acellular matrix is prepared by performing cleaning, peroxyacetic acid virus inactivation, supercritical carbon dioxide decellularization and degreasing treatment on adipose tissues, and splitting the adipose tissues into particles with proper particle sizes. The material can be used as a drug sustained-release carrier material, has good biocompatibility, excellent drug loading and sustained-release performance and degradability, and can be widely applied to sustained release of various drugs including antibiotics, antitumor drugs and the like. The invention provides a novel and potential material source and a preparation method for the field of drug sustained release.

The application relates to the field of biomaterials, and particularly discloses a kind of acellular matrix material and a preparation method thereof.The preparation method of the material comprises the following steps: extracting tissue material of a mammal, performing acellular treatment on the tissue material to obtain acellular tissue material, freeze-drying the tissue material, and then crushing the freeze-dried tissue material to obtain animal-derived acellular matrix; the animal-derived acellular matrix is resuspended in water, mixed with a modified epoxidized chitosan solution, and then transglutaminase is added to perform a reaction; the product obtained is freeze-dried to obtain the acellular matrix material.The method can improve the biocompatibility of the acellular matrix and reduce the immune rejection reaction by cross-linking the animal-derived acellular matrix with modified epoxidized chitosan; at the same time, since the cross-linking reaction is a biological cross-linking reaction under the action of transglutaminase, the biological activity factors contained in the acellular matrix are not easily destroyed during the cross-linking process, and the biological activity and safety are better.

The application provides an injectable hydrogel for endometrial repair and / or prevention of adhesion of uterine cavity and a preparation method and application thereof, and belongs to the technical field of biomedical materials. The injectable hydrogel is composed of a base gel and a decellularized matrix distributed in the base gel; the base gel is composed of a cross-linked carboxymethyl chitosan gel and a carbomer gel; the base gel has an interpenetrating or semi-interpenetrating double network structure; and the cross-linked carboxymethyl chitosan gel is a chemical cross-linked carboxymethyl chitosan gel. In the application, the injectable hydrogel forms a base gel with an interpenetrating or semi-interpenetrating double network structure by using a cross-linked carboxymethyl chitosan gel and a carbomer gel, and is compounded with a decellularized matrix, so that the injectable hydrogel has high elasticity and high adhesion, can effectively prevent adhesion and repair damaged tissues, has a long action time, and has good biocompatibility.

This invention relates to a decellularized oral collagen plug and its preparation method. The plug comprises an upper decellularized matrix membrane and a lower decellularized sponge plug. The preparation method is as follows: pretreated fresh animal-derived membrane material is treated with trypsin solution and sodium hydroxide solution, washed with purified water to obtain a decellularized matrix membrane; a portion of the obtained decellularized matrix membrane is placed in a low-temperature tissue ball mill, pulverized, homogenized, filtered, and washed with purified water to obtain a decellularized collagen solution; the decellularized collagen solution is poured into a freeze-drying mold, defoamed under low-temperature vacuum, covered with the decellularized matrix membrane, and after vacuum defoaming, freeze-dried and vacuum thermally cross-linked to obtain the finished collagen plug. This invention's decellularized oral collagen plug is suitable for filling and covering wounds after tooth extraction. The upper decellularized matrix membrane effectively prevents fibroblasts and epithelial cells from invading bone defect areas, while the lower decellularized sponge plug stabilizes blood clots, provides a biological scaffold, reduces postoperative complications, and promotes wound healing.

This invention discloses a biomimetic decellularized matrix hydrogel and its applications. It relates to the field of biomaterials technology. It comprises the following components: decellularized liver matrix, gelatin, and sodium alginate, and its applications are provided. The therapeutic artificial liver provided by this invention can be mass-produced in vitro using 3D bioprinting technology, exhibiting mature liver functional phenotypes such as ICG uptake / release and drug metabolism in vitro. It was transplanted into a type I tyrosinemia model of liver failure (Fah). ‑ / ‑ It can alleviate liver damage in mice and has the potential for artificial liver transplantation.

The invention provides a preparation method of a collagen-based bone filling material, which comprises the following steps: S1) heating an acellular matrix biological membrane for thermal crosslinking treatment, and crushing to obtain matrix powder; and S2) mixing the matrix powder with a solvent, performing whipping treatment, then adding inorganic bone particles, performing mixing, and performing freeze drying and thermal crosslinking treatment to obtain the collagen-based bone filling material. Compared with the prior art, the collagen-based bone filling material provided by the invention is composed of the acellular matrix biological membrane and the natural inorganic bone particles, and the acellular matrix biological membrane is subjected to thermal crosslinking treatment, crushing, swelling and whipping treatment to form a stable three-dimensional network structure; the bone filling material prepared by the preparation method disclosed by the invention can obviously prolong the in-vitro degradation time after being physically crosslinked with inorganic bone particles, and the bone filling material has no chemical reagent residue, not only has good biocompatibility, but also has good rebound resilience and plasticity, and is convenient to operate.

The application belongs to the technical field of nerve grafts, and discloses a multilayer composite capacitive film, a preparation method and application of the multilayer composite capacitive film in nerve repair scaffolds. The scaffold is composed of three layers, namely, an A layer, a B layer and an A layer. The A layer is a sandwiched layer on both sides, and comprises a mixture of chitosan (or silk fibroin or a decellularized matrix) and a conductive polymer PEDOT:PSS and a crosslinking agent polyethylene glycol diglycidyl ether (PEGDE). The B layer is a middle hydrogel sandwiched layer, and comprises methacrylated gelatin (Gel-MA). The A layers on both sides are bonded through the B layer to form a viscoelastic hydrogel after ultraviolet light irradiation, and the composite capacitive scaffold in a multilayer structure with a through middle cavity is obtained after curing and air drying. The formed scaffold has good electrical conductivity, mechanical properties and biological safety, and can effectively induce the proliferation, migration and differentiation of RSC96 Schwann cells and nerve system cells PC12, thereby providing a good physical stable support and a favorable microenvironment for peripheral nerve injury repair.

The invention discloses a functionalized acellular matrix material and a preparation method thereof, and relates to the technical field of biomedical treatment, the functionalized acellular matrix material comprises: a composite ECM skeleton, which is prepared by compounding squid sheaths and amniotic membranes and performing acellular treatment; the multi-stage response degradation switch comprises a matrix metalloproteinase sensitive peptide cross-linking bridge and a polythioether-polylactic acid block copolymer oxidation response chain segment; the natural biological conductive network is formed by compounding an in-situ polymerization product of a mussel byssus protein conductive fragment and amino-modified graphene quantum dots, the characteristics of squid sheaths and amniotic membranes are fused to construct a composite ECM skeleton, a multi-stage response degradation switch is integrated, and the comprehensive performance of the material is optimized; meanwhile, a natural biological conductive network is introduced, so that the electroactivity is improved, and a safe, efficient and powerful novel stent solution is provided for tissue engineering.

The invention relates to the technical field of biomedicine, in particular to a method for preparing an acellular matrix from novel oligopeptide MY-1 and application of the acellular matrix. The preparation method comprises the following steps: (1) separation and culture of mesenchymal stem cells; (2) culturing under the condition of an MY-1 stimulating factor to obtain an extracellular matrix; and (3) carrying out decellularization treatment to obtain the decellularized matrix. The preparation method is simple, the prepared specific dECM can remarkably promote in-vitro migration and directional differentiation of mesenchymal stem cells, osteoporotic femoral defect repair can be highly promoted, and the performance of the dECM is better than that of dECM cultured through conventional culture or addition of PTH or knock-down type III collagen.

The invention discloses an oral squamous cell carcinoma decellularized matrix and a preparation method of dECM hydrogel, and the preparation method of the oral squamous cell carcinoma decellularized matrix comprises the following steps: soaking the tissue in a 0.1% w / v SDS solution for 28 hours, magnetically stirring at room temperature, and replacing the SDS solution every 12 hours; deionized water is used for cleaning for at least one hour to remove SDS residual liquid; soaking and eluting residual nucleic acid fragments by using 40U / mL nuclease, and magnetically stirring at 37 DEG C for 3 hours; and washing with deionized water for at least 1 hour, and storing the obtained oral squamous cell carcinoma acellular matrix at-80 DEG C for later use. The invention also discloses application of the dECM hydrogel in preparation of a three-dimensional cell culture system and in-vitro biological models with different invasion abilities.

The present application relates to the technical field of regenerative medicine, in particular to a self-assembled stem cell material composite spheroid and a preparation method and application thereof.The preparation method comprises the following steps: preparing a primary mesenchymal stem cell suspension; taking animal tissue and preparing a decellularized matrix microparticle suspension through decellularization treatment; mixing a nanosheet solution and a TGF-beta solution and incubating to obtain a factor-loaded nanosheet solution; uniformly mixing the factor-loaded nanosheet solution and the decellularized matrix microparticle suspension, cross-linking and compounding to obtain cross-linked composite microparticles; and mixing the cross-linked composite microparticle suspension and the stem cell suspension and culturing to obtain a self-assembled stem cell material composite spheroid.The self-assembled stem cell material composite spheroid has a high survival rate when implanted into an intervertebral disc, controllable differentiation, and a good clinical application prospect, and provides a new idea for the treatment of degenerative intervertebral discs and other degenerative diseases.

The invention belongs to the technical field of biomedical hydrogel, and particularly relates to a wet adhesion tissue specific hydrogel patch and a preparation method thereof. The wet adhesion tissue specific hydrogel patch is prepared from methacrylated silk fibroin, a methacrylated acellular matrix, phenyl (2, 4, 6-trimethylbenzoyl) lithium phosphate and tannic acid, and the wet adhesion tissue specific hydrogel patch is prepared from the methacrylated silk fibroin, the methacrylated acellular matrix, the phenyl (2, 4, 6-trimethylbenzoyl) lithium phosphate and the tannic acid. The preparation method mainly comprises the steps of hydrogel solution preparation, illumination crosslinking and tannic acid soaking. The preparation method can be used for flexibly preparing a double-sided or single-sided adhesive patch. The hydrogel patch disclosed by the invention has excellent wet adhesion performance, tissue specificity, biocompatibility and mechanical property, and is remarkable in hemostatic effect; the hydrogel patch can avoid secondary injury and foreign matter inflammation risks caused by traditional suture, realizes rapid closing of wet wound surfaces, efficient hemostasis and tissue repair, is suitable for scenes such as surgical operations and wound first aid, and has important clinical application value.

The application discloses a three-layer time sequence regulation type inflammation targeting composite repair system for repairing endometrial injury and a preparation method and application thereof. The system is a three-layer composite structure, which comprises, from outside to inside, (1) an outer ROS response hydrogel layer loaded with anti-inflammatory drugs, which can respond to early high expression of active oxygen to rapidly degrade the drugs, so that acute inflammation is inhibited; (2) a middle layer of targeted delivery and metabolic repair, which is a M1 macrophage membrane pseudo-loaded drug liposome (NMN@M-Lipo), which is exposed after the degradation of the outer hydrogel, actively targets the inflammation lesion through the M1 membrane, and slowly releases nicotinamide mononucleotide (NMN) to restore cell energy metabolism; and (3) an inner layer of cell recruitment and regeneration, which is a decellularized matrix scaffold loaded with SDF-1 alpha, which continuously releases SDF-1 alpha to recruit endogenous stem cells in the later repair stage, and promotes functional regeneration of the endometrium. The application utilizes the time sequence microenvironment changes of endometrial repair, and realizes the cascade regulation of 'anti-inflammation first, metabolic repair second, and regeneration promotion third'. Animal experiments show that the system can significantly restore the reproductive function of the injured endometrium and improve the number of offspring.

The invention discloses application of an acellular matrix material as a tissue damage or tumor targeting probe or carrier. A preparation method of the acellular matrix material comprises the following steps: pretreating fish tissues; performing decellularization treatment on the pretreated fish tissue to obtain a decellularized fish tissue material; the preparation method comprises the following steps: freeze-drying, grinding and digesting the decellularized fish tissue material, then carrying out ultrafiltration and centrifugation to intercept filtrate with the molecular weight of less than 50KDa, and freeze-drying to obtain the decellularized matrix material. The acellular matrix material disclosed by the invention has good blood compatibility and has a targeting effect on tissue injury, especially myocardial injury parts, diabetic nephropathy tissues or tumors.

The application relates to the field of hydrogels, in particular to a decellularized matrix composite hydrogel dressing for acute and chronic wound healing and a preparation method thereof, which comprises the following steps: S1, taking a pig spleen decellularized matrix and configuring the same into a hydrogel solution, dropping methacrylic anhydride under room temperature conditions, dialyzing for 4 days under 4 DEG C, freeze-drying the solution, and obtaining PECM-MA; S2, dissolving hyaluronic acid, 1-ethyl-(3-dimethylaminopropyl) carbonyldiimidazole hydrochloride and N-hydroxysuccinimide in a MES buffer solution, stirring and dissolving to form a solution one; dissolving L-cysteine in the MES buffer solution, stirring to form a solution two, adding the solution two into the solution one, reacting for 12-24 hours under room temperature, dialyzing the reaction liquid for 3 days, and freeze-drying to obtain a product HA-SH; S3, taking the PECM-MA and the HA-SH, adding carboxymethyl chitosan into deionized water, dissolving and stirring uniformly to form a hydrogel. The natural biomaterials are combined with the high polymer materials, the advantages of both are combined, and the hydrogel has good viscoelasticity and rapid relaxation performance.