44 results about "Decellularized matrix" patented technology

By means of the method according to one aspect, in a process of enhancing biocompatibility of an acellular matrix by supercritical carbon dioxide treatment, immunogenicity of the acellular matrix can be reduced with effective efficiency while maintaining a content of a physiologically active substance and physiologically active ability. Accordingly, the acellular matrix prepared by the method according to one aspect, the composition including the same, and the biomaterial have low biotoxicity and excellent tissue regeneration energy, and thus can be usefully used in cosmetic or medical materials.

The invention discloses an antibacterial composite periosteum for an oral cavity and a processing technology of the antibacterial composite periosteum, and relates to the technical field of periosteum. The method specifically comprises the following steps: step 1, treating defatted pig peritoneum with a decellularized solution, cleaning and sterilizing to obtain a pig peritoneum decellularized matrix; 2, treating the pig peritoneal decellularized matrix with a glyoxal solution, cleaning, and sterilizing to obtain a pretreated pig peritoneal decellularized matrix; step 3, uniformly coating the surface of the pretreated porcine peritoneal acellular matrix with an antibacterial solution, and performing sterilization, standing and freeze-drying to obtain the antibacterial composite periosteum. Wherein the antibacterial solution is prepared from the following raw material components in concentration: 1 to 2 weight percent of modified chitosan, 0.2 to 0.4 weight percent of amino-terminated polyethylene glycol and 0.01 to 0.05 weight percent of p-toluenesulfonic acid, and a solvent is a PBS (Phosphate Buffer Solution). The antibacterial composite periosteum is good in biocompatibility and has excellent clinical application value in oral surgery.

This invention relates to the field of biomedical materials technology, specifically to a decellularized matrix microparticle (dECM-MPs) 3D printing ink and its preparation method. The key technical points are: a heat-absorbing compound is temporarily dissolved on the surface of decellularized matrix microparticles (dECM-MPs) via recrystallization from a supersaturated solution; then, the microparticles are immersed in a temperature-sensitive gelatin solution. The heat absorbed during the dissolution of the heat-absorbing compound leads to a localized low temperature around the microspheres, causing the gelatin to solidify on the dECM-MPs surface, forming a physically cured gelatin hydrogel microlayer in situ. This microlayer is further stabilized by enzymatic cross-linking. Photosensitive modified hyaluronic acid molecules are introduced into the hydrogel microlayer to regulate its mechanical properties. Centrifugation yields a particulate ink suitable for extrusion 3D printing. After 3D printing, ultraviolet light irradiation is used to produce a 3D printed implant. This invention overcomes the problem that rigid dECM-MPs, such as decellularized cartilage microparticles or decellularized bone microparticles, cannot be extruded and printed. By regulating the mechanical properties of the hydrogel microlayer, particulate ink with excellent printing performance can be obtained. This method overcomes the limitation of rigid tissues being difficult to additively manufacture.

The application discloses a preparation method of a decellularized matrix and a product prepared by the method. The preparation method comprises the following steps: (1) washing a mammalian soft tissue to remove blood, lipids and impurities, and cutting the tissue into pieces with a thickness of 1-10 mm; (2) soaking the pieces in solution 1 for 6-48 h, and then soaking the pieces in solution 2 for 0.1-1 h; (3) treating the pieces in an ultrasonic water bath under the condition of a frequency of 20-40 kHz for 5-30 min; (4) water-bathing the pieces at 55-65 DEG C for 10-30 min; (5) sequentially washing the tissue with deionized water, a PBS buffer and ethanol, and washing the tissue with each solution for 1-3 times, each time for 10-30 min; and (6) freeze-drying the decellularized matrix material, and crushing the material at-80 DEG C to-20 DEG C to obtain a decellularized matrix powder. The preparation method significantly improves the cell component removal efficiency, effectively protects structural proteins such as collagen from being degraded in the freeze-drying and crushing processes, and ensures the integrity of the matrix in the aspects of morphology, structure and function, thereby providing a structural support and biological signal support for subsequent tissue repair.

The present application belongs to the field of biomedical materials and tissue engineering, and particularly relates to a kind of acellular matrix aerogel repair material and its preparation method and application.Further, the present application relates to the comprehensive preparation method of the material, especially the extremely low immunogenicity acellular process combined with physical, chemical and supercritical fluid technologies, and the unique aerogel forming technology based on supercritical drying.Further, the present application relates to the application of the material, mainly related to soft tissue repair, medical cosmetology, bone / cartilage injury repair field.

The application provides an injectable hydrogel for endometrial repair and / or prevention of adhesion of uterine cavity and a preparation method and application thereof, and belongs to the technical field of biomedical materials. The injectable hydrogel is composed of a base gel and a decellularized matrix distributed in the base gel; the base gel is composed of a cross-linked carboxymethyl chitosan gel and a carbomer gel; the base gel has an interpenetrating or semi-interpenetrating double network structure; and the cross-linked carboxymethyl chitosan gel is a chemical cross-linked carboxymethyl chitosan gel. In the application, the injectable hydrogel forms a base gel with an interpenetrating or semi-interpenetrating double network structure by using a cross-linked carboxymethyl chitosan gel and a carbomer gel, and is compounded with a decellularized matrix, so that the injectable hydrogel has high elasticity and high adhesion, can effectively prevent adhesion and repair damaged tissues, has a long action time, and has good biocompatibility.

The application belongs to the technical field of nerve grafts, and discloses a multilayer composite capacitive film, a preparation method and application of the multilayer composite capacitive film in nerve repair scaffolds. The scaffold is composed of three layers, namely, an A layer, a B layer and an A layer. The A layer is a sandwiched layer on both sides, and comprises a mixture of chitosan (or silk fibroin or a decellularized matrix) and a conductive polymer PEDOT:PSS and a crosslinking agent polyethylene glycol diglycidyl ether (PEGDE). The B layer is a middle hydrogel sandwiched layer, and comprises methacrylated gelatin (Gel-MA). The A layers on both sides are bonded through the B layer to form a viscoelastic hydrogel after ultraviolet light irradiation, and the composite capacitive scaffold in a multilayer structure with a through middle cavity is obtained after curing and air drying. The formed scaffold has good electrical conductivity, mechanical properties and biological safety, and can effectively induce the proliferation, migration and differentiation of RSC96 Schwann cells and nerve system cells PC12, thereby providing a good physical stable support and a favorable microenvironment for peripheral nerve injury repair.

The invention discloses a functionalized acellular matrix material and a preparation method thereof, and relates to the technical field of biomedical treatment, the functionalized acellular matrix material comprises: a composite ECM skeleton, which is prepared by compounding squid sheaths and amniotic membranes and performing acellular treatment; the multi-stage response degradation switch comprises a matrix metalloproteinase sensitive peptide cross-linking bridge and a polythioether-polylactic acid block copolymer oxidation response chain segment; the natural biological conductive network is formed by compounding an in-situ polymerization product of a mussel byssus protein conductive fragment and amino-modified graphene quantum dots, the characteristics of squid sheaths and amniotic membranes are fused to construct a composite ECM skeleton, a multi-stage response degradation switch is integrated, and the comprehensive performance of the material is optimized; meanwhile, a natural biological conductive network is introduced, so that the electroactivity is improved, and a safe, efficient and powerful novel stent solution is provided for tissue engineering.

The present application relates to the technical field of regenerative medicine, in particular to a self-assembled stem cell material composite spheroid and a preparation method and application thereof.The preparation method comprises the following steps: preparing a primary mesenchymal stem cell suspension; taking animal tissue and preparing a decellularized matrix microparticle suspension through decellularization treatment; mixing a nanosheet solution and a TGF-beta solution and incubating to obtain a factor-loaded nanosheet solution; uniformly mixing the factor-loaded nanosheet solution and the decellularized matrix microparticle suspension, cross-linking and compounding to obtain cross-linked composite microparticles; and mixing the cross-linked composite microparticle suspension and the stem cell suspension and culturing to obtain a self-assembled stem cell material composite spheroid.The self-assembled stem cell material composite spheroid has a high survival rate when implanted into an intervertebral disc, controllable differentiation, and a good clinical application prospect, and provides a new idea for the treatment of degenerative intervertebral discs and other degenerative diseases.

The invention belongs to the technical field of biomedical hydrogel, and particularly relates to a wet adhesion tissue specific hydrogel patch and a preparation method thereof. The wet adhesion tissue specific hydrogel patch is prepared from methacrylated silk fibroin, a methacrylated acellular matrix, phenyl (2, 4, 6-trimethylbenzoyl) lithium phosphate and tannic acid, and the wet adhesion tissue specific hydrogel patch is prepared from the methacrylated silk fibroin, the methacrylated acellular matrix, the phenyl (2, 4, 6-trimethylbenzoyl) lithium phosphate and the tannic acid. The preparation method mainly comprises the steps of hydrogel solution preparation, illumination crosslinking and tannic acid soaking. The preparation method can be used for flexibly preparing a double-sided or single-sided adhesive patch. The hydrogel patch disclosed by the invention has excellent wet adhesion performance, tissue specificity, biocompatibility and mechanical property, and is remarkable in hemostatic effect; the hydrogel patch can avoid secondary injury and foreign matter inflammation risks caused by traditional suture, realizes rapid closing of wet wound surfaces, efficient hemostasis and tissue repair, is suitable for scenes such as surgical operations and wound first aid, and has important clinical application value.

The application discloses a three-layer time sequence regulation type inflammation targeting composite repair system for repairing endometrial injury and a preparation method and application thereof. The system is a three-layer composite structure, which comprises, from outside to inside, (1) an outer ROS response hydrogel layer loaded with anti-inflammatory drugs, which can respond to early high expression of active oxygen to rapidly degrade the drugs, so that acute inflammation is inhibited; (2) a middle layer of targeted delivery and metabolic repair, which is a M1 macrophage membrane pseudo-loaded drug liposome (NMN@M-Lipo), which is exposed after the degradation of the outer hydrogel, actively targets the inflammation lesion through the M1 membrane, and slowly releases nicotinamide mononucleotide (NMN) to restore cell energy metabolism; and (3) an inner layer of cell recruitment and regeneration, which is a decellularized matrix scaffold loaded with SDF-1 alpha, which continuously releases SDF-1 alpha to recruit endogenous stem cells in the later repair stage, and promotes functional regeneration of the endometrium. The application utilizes the time sequence microenvironment changes of endometrial repair, and realizes the cascade regulation of 'anti-inflammation first, metabolic repair second, and regeneration promotion third'. Animal experiments show that the system can significantly restore the reproductive function of the injured endometrium and improve the number of offspring.

The invention discloses application of an acellular matrix material as a tissue damage or tumor targeting probe or carrier. A preparation method of the acellular matrix material comprises the following steps: pretreating fish tissues; performing decellularization treatment on the pretreated fish tissue to obtain a decellularized fish tissue material; the preparation method comprises the following steps: freeze-drying, grinding and digesting the decellularized fish tissue material, then carrying out ultrafiltration and centrifugation to intercept filtrate with the molecular weight of less than 50KDa, and freeze-drying to obtain the decellularized matrix material. The acellular matrix material disclosed by the invention has good blood compatibility and has a targeting effect on tissue injury, especially myocardial injury parts, diabetic nephropathy tissues or tumors.

The embodiment of the application discloses a preparation method of humanized extracellular matrix, and belongs to the technical field of biomedical materials and tissue engineering. The method comprises the following steps: preparing human decellularized dermal matrix; preparing a concentrated solution of a condition medium rich in human active factors; constructing RGD-PLGA / BMP-2 nanocomposites; loading the concentrated solution of the condition medium into the human decellularized dermal matrix through vacuum negative pressure perfusion, and then anchoring the RGD-PLGA / BMP-2 nanocomposites, and cross-linking them twice through genipin to obtain the humanized extracellular matrix. Through the four-element strategy of decellularized matrix-active factor-nano-sustained release-two-stage cross-linking, the unity of low immunogenicity, high biological activity and spatiotemporal controllable release function is realized, and the humanized extracellular matrix can be applied to the fields of tissue engineering scaffolds, wound repair and bone defect filling.

The invention provides a preparation method and application of dual-network gel for cartilage repair. The preparation method comprises the following steps: mixing an acellular matrix solution with an acrylamide monomer, adding a first cross-linking agent, and carrying out primary cross-linking to form a first network structure; then, a second cross-linking agent is added for secondary cross-linking, and the polyacrylamide / acellular matrix double-network gel is formed. According to the invention, the acellular matrix and polyacrylamide are subjected to dual-network combination, collaborative optimization of biological activity and mechanical properties is realized, and the composite strategy not only avoids the immune risk of a pure synthetic material, but also breaks through the mechanical bottleneck when the acellular matrix is independently used. Besides, by regulating and controlling the formula, the crosslinking strength can be accurately regulated, the degradation rate can be matched and is synchronized with the cartilage regeneration period of 6-12 months, and the defect that degradation of a traditional material is uncontrollable is overcome.

The application discloses a preparation method of umbilical cord decellularized matrix based on perfusion decellularization technology, which comprises decellularization treatment of a complete umbilical cord by using a programmed perfusion method, and cleaning, enzymatic hydrolysis, freeze-drying and other processes. The application further discloses the umbilical cord decellularized matrix prepared by the method and application thereof. The obtained umbilical cord decellularized matrix has complete structural integrity, retains not only umbilical cord Wharton's jelly but also rich low-immunogenic extracellular matrix components in umbilical cord tissues, and has wide application prospects in the development of soft tissue injury repair, medical beauty and life beauty related products.

The invention belongs to the technical field of biomedical materials, and particularly relates to a clinical ready-to-use autologous adipose tissue-derived matrix collagen membrane and application thereof. The clinical ready-to-use autologous adipose tissue-derived matrix collagen membrane provided by the invention is mainly composed of autologous adipose tissue-derived collagen, and contains a plurality of key adhesion proteins, glycosaminoglycans, growth factors and other bioactive components. The preparation method comprises the following steps: by taking autologous adipose tissue as a raw material, breaking cells by a mechanical method, repeatedly cleaning to remove cell components such as cell debris and oil drops, and mechanically pressing through a mold to form a diaphragm with uniform thickness, regular shape and rough surface. The membrane effectively overcomes the limitation of the existing acellular matrix biological membrane, can load various bioactive substances to enhance the function, can be stored at low temperature for a long time without influencing the repair effect, and greatly enhances the convenience of subsequent use.

The invention relates to the technical field of acellular matrix gel filler preparation, and discloses an acellular matrix gel filler raw material grinding device and a gel filler. The acellular matrix gel filler raw material grinding device comprises a grinding tank, the inner wall of the interior of the grinding tank is further provided with a cold storage cavity used for storing liquid nitrogen, and the cold storage cavity is internally provided with a cold storage cavity used for storing liquid nitrogen; a grinding cavity and two pushing parts are arranged in the grinding tank, the pushing parts are rotationally connected to the grinding cavity, a processing cavity for raw material circulation is formed between the two pushing parts, and the pushing parts are used for grinding newly added raw materials. Raw materials are thrown upwards to impact on water absorption materials, so that separation is achieved, the raw materials and the water absorption materials do not influence each other, then only when the raw materials which are completely dehydrated and frozen become hard, the raw materials impact the cylinder and then are ejected to the annular groove, all the raw materials are qualified to-be-ground raw materials, the overall front work is shortened, and the grinding efficiency is improved.

The application relates to the field of biological medicine, in particular to a composition with pH response release antibacterial polypeptide, a preparation method and application thereof. The composition comprises: a decellularized matrix material, an activator, a grafting agent and antibacterial polypeptide. The decellularized matrix and the antibacterial polypeptide are connected through an imine bond, the imine bond is hydrolyzed under acidic conditions, the antibacterial polypeptide is released, and the imine bond is reconnected under a neutral environment; the process is dynamically reversible, so that the antibacterial polypeptide is released in a responsive manner. The dressing can promote the repair and regeneration of a wound, and simultaneously inhibit the growth and proliferation of bacteria, and has great application potential.

The invention relates to a preparation method and application of a gentamicin sulfate acellular matrix bacteriostatic slice, and the preparation method comprises the following steps: S1, taking an animal skin material, degreasing, washing and cutting to obtain a tissue material; s2, immersing the tissue material in sterile water for multiple times of ultrasonic cleaning, and then adding the tissue material into a cleaning solution for multiple times of soaking and cleaning to obtain a decellularized tissue; s3, freeze-drying the decellularized tissue, and grinding at-20 to 0 DEG C to obtain decellularized matrix powder; s4, adding the acellular matrix powder, collagen powder and gentamicin sulfate into normal saline, and uniformly mixing to obtain composite gel; and S5, pouring the composite gel into a mold, spreading the composite gel, performing pre-compression molding, and performing freeze-drying to obtain the gentamicin sulfate acellular matrix antibacterial slice. The acellular matrix treated by combining a physical means and a chemical means is cooperated with the collagen and gentamicin sulfate, so that the antibacterial sheet has the effects of high biocompatibility, long-acting and stable antibacterial effect and excellent mechanical property.

The application provides a preparation method of a collagen-based bone filling material, comprising the following steps: S1) heating and performing heat crosslinking treatment on a decellularized matrix biomembrane, and then crushing the decellularized matrix biomembrane to obtain a matrix powder; S2) mixing the matrix powder with a solvent to perform whipping treatment, then adding inorganic bone particles to mix, and then performing freeze-drying and heat crosslinking treatment to obtain the collagen-based bone filling material. Compared with the prior art, the collagen-based bone filling material provided by the application is composed of a decellularized matrix biomembrane and natural inorganic bone particles, wherein the decellularized matrix biomembrane is subjected to heat crosslinking treatment, and then is subjected to crushing, swelling and whipping treatment, so that a stable three-dimensional network structure is formed; after physical crosslinking of the inorganic bone particles, the in-vitro degradation time is significantly prolonged; the bone filling material has no chemical reagent residue, has good biocompatibility, good resilience and plasticity, and is convenient to operate.

The invention belongs to the technical field of biological products, and particularly relates to a bionic biological 3D printing active stent, a preparation method and application. The method comprises the following steps: respectively preparing decellularized matrix hydrogel (DSCM-G) from spinal cord tissues and decellularized matrix hydrogel (DNM-G) from peripheral nerve tissues, preparing a partitioned spinal cord bionic scaffold or a partitioned mold through 3D printing, then respectively pouring DSCM-G containing or not containing cells into a central region, pouring DNM-G containing or not containing cells into peripheral regions, and finally, preparing the partitioned spinal cord bionic scaffold or the partitioned mold through 3D printing. And gelatinizing and curing to obtain the multi-bionic spinal cord active stent. Bionic construction of grey matter and white matter is achieved through different biological materials and cells, spatial distribution construction of the grey matter area and the white matter area is achieved through the 3D printing technology, the components, modulus, micro-network structure and function of the material are closer to those of spinal cord tissue, good biocompatibility is achieved, and the material can be applied to the field of spinal cord tissue engineering. And synchronous collaborative recovery of grey matter and white matter after spinal cord injury is more likely to be realized.

A preparation method for a decellularized matrix biomaterial, which combines specific pretreatment, degreasing, disinfection, decellularization, α-Gal antigen removal and sterilization, and reserves the integrity of bioactive components and structures in a material to the maximum extent while effectively removing an antigenic substance of an animal tissue, so that a decellularized matrix biomaterial having low immunogenicity and high biological activity is obtained. In addition, the decellularized matrix biomaterial prepared by the method effectively reduces immunogen components, has good biocompatibility and biological safety, is beneficial to cell adhesion, aggregation and proliferation on the surface, and can be used as a high-activity biological matrix material to be applied to skin soft tissue defect, gingival recession and periodontal soft tissue repair.

The invention provides an amnion decellularized matrix method. The amnion decellularized matrix method comprises the steps of amnion pretreatment, circulating freeze thawing treatment and purification treatment. According to the process, a physical method and a biological method are cooperated, cells are removed through low-temperature freezing and thawing of freezing at the temperature of-200 DEG C to-195 DEG C and water bath thawing at the temperature of 37 DEG C in a liquid nitrogen environment in cooperation with an enzymatic degradation method, shallow and deep cells are removed at the same time, the decellularization efficiency is extremely high, and the finally obtained amniotic membrane decellularization matrix is free of visible nuclear cells. Compared with the prior art, the method overcomes the defects of incomplete decellularization, long treatment time and easy fragmentation of tissues in a physical method, and shortens the preparation time; harmful chemical reagents are not needed, the problems of chemical method reagent residual toxicity and matrix structure damage are avoided, meanwhile, the inflammation risk in the biological method preparation process is reduced, the natural component retention rate is high, and reagent residues are avoided; the finally obtained sterile amnion acellular matrix has the advantages of good biocompatibility, guarantee of clinical safety, simple process and convenience in popularization and implementation.

The present application provides a degradable in-vivo pressure-sensitive adhesive taking a decellularized matrix material as a base material and a preparation method therefor. The degradable in-vivo pressure-sensitive adhesive comprises a biological base material layer and pressure-sensitive adhesive layers arranged on both sides of the biological base material layer. On the basis of the total mass of raw materials of the pressure-sensitive adhesive layer as 100%, the raw materials of the pressure-sensitive adhesive layer comprise, by mass percentage, 5%-18% of a tissue-active material, 5%-25% of a base material, 10%-35% of a thickener, 10%-50% of a plasticizer, and 10%-60% of deionized water. The degradable in-vivo pressure-sensitive adhesive provided by the present application not only has good compatibility with human tissues, but also has adjustable viscosity. Moreover, the degradable in-vivo pressure-sensitive adhesive can be absorbed or completely discharged out of the body after degradation in vivo. Therefore, it is suitable for in-vivo wound closure, prevention of fluid (gas) leakage at suture sites, tissue repair, drug delivery, wound healing, and other applications.

This invention belongs to the field of biomedical materials and tissue engineering technology, and provides a decellularized matrix composite hydrogel dressing, its preparation method, and its application. The decellularized matrix composite hydrogel dressing provided by this invention includes a hydrogel matrix and ginger exosome-like nanoparticles (GELNs) dispersed in the hydrogel matrix; the hydrogel matrix is ​​prepared from raw materials including decellularized dermal matrix and decellularized aortic adventitia matrix. The composite hydrogel dressing of this invention has a three-dimensional porous structure, enabling sustained release of GELNs. It also significantly accelerates the healing process of full-thickness skin defects by downregulating the TLR4 / MyD88 / NF-κB signaling pathway, synergistically regulating macrophage polarization towards the M2 type, and simultaneously promoting angiogenesis and collagen deposition. The dressing has widely available raw materials, good biocompatibility, and broad application prospects in the field of skin wound repair.

The invention provides an acellular matrix membrane, a preparation method and application, the acellular matrix membrane comprises at least one of collagen, laminin, glycosaminoglycan and keratin, the collagen comprises type I collagen, the type I collagen is of a triple helix structure, and the light transmittance of the acellular matrix membrane is 80%-99%. The transparent acellular matrix membrane is prepared from a conventional opaque animal-derived membrane material through a special transparent treatment process, the light transmittance is improved, the microstructure of collagen is effectively reserved, cell crawling and proliferation are facilitated, and the acellular matrix membrane has a good bioremediation function and also has a good application prospect. And the real-time visual observation of the wound regeneration process in clinic is also facilitated.

The present application relates to an injectable thermosensitive pre-gel solution, and a preparation method therefor and a use thereof. Raw materials for preparing the injectable thermosensitive pre-gel solution comprise a decellularized matrix and a thermosensitive material. The injectable thermosensitive pre-gel solution is prepared by a method comprising the following steps: freezing and pulverizing the decellularized matrix to obtain decellularized matrix powder; mixing the decellularized matrix powder with an acidic solution to obtain a mixed solution; and mixing the mixed solution with the thermosensitive material to obtain the injectable thermosensitive pre-gel solution. The preparation method for the injectable thermosensitive pre-gel solution is simple; when injected into the body, the injectable thermosensitive pre-gel solution can quickly form a gel; the formed hydrogel has high safety and strong applicability, and can specifically repair damaged tissues and organs in the human body and induce tissue regeneration and repair.

The invention discloses temperature-sensitive acellular matrix hydrogel and a preparation method and application thereof, and relates to the technical field of material preparation.The method comprises the following steps that human bone marrow mesenchymal stem cells are added into a serum-free culture medium containing ascorbic acid to be cultured, and a biomass matrix material is obtained; adding the biomass matrix material into a decellularization solution for decellularization treatment to obtain a decellularization matrix membrane; carrying out freeze drying and grinding treatment on the acellular matrix membrane to obtain acellular matrix powder; adding the acellular matrix powder into a pepsin hydrochloric acid solution, and uniformly mixing to obtain an acellular matrix solution; adjusting the pH value and osmotic pressure of the acellular matrix solution, placing the acellular matrix solution at 37 DEG C to form gel, and storing the gel at 4 DEG C to obtain the temperature-sensitive acellular matrix hydrogel; the hydrogel with natural extracellular matrix characteristics prepared by the invention can realize rapid liquid-solid phase transformation at body temperature, and has the advantages of high biocompatibility, excellent temperature response performance and capability of effectively promoting tissue repair.