46 results about "Decellularized matrix" patented technology

Decellularized tissue-derived extracellular matrices (ECM) and methods of generating and using same are provided. The method of generating a decellularized matrix includes the steps of: (a) subjecting the tissue to washes and a hypertonic buffer; (b) subjecting the tissue to an enzymatic proteolytic digestion with an enzyme such as trypsin; and (c) removing all cellular components from the tissue using a detergent solution which includes Triton-X-100 and ammonium hydroxide. Specifically, there is provided a decellularized myocardium-derived matrix which is completely devoid of all cellular components and hence non-immunogenic in a subject, exhibits suitable structural and mechanical properties for cardiac tissue engineering or replacement therapy of damaged cardiac tissue, and is capable of remodeling upon seeding of cells.

The invention relates to a chitosan nano-material, particularly a nano-material formed with heparin. The invention further relates to a cell matrix-removed biomaterial. The invention aims at providing chitosan-heparin nano-particles suitable for specific binding of vascular endothelial growth factor (VEGF) and heparin and capable of binding with the cell matrix-removed biomaterial. The biomaterial is prepared by using an ion cross-linking method. The biological valve-possessed duct for pulmonary artery vessel restoration or reconstruction provided by the invention has good endothelialization performance, antithrombotic performance, anti-bacteria performance, and long-term persistent curative effect, and is beneficial for self-replacement of the biological valve-possessed duct.

The invention discloses a preparation method of a natural soft tissue decellularization matrix. The method includes material taking, pretreatment, disinfection, degreasing, enzyme treatment, decellularization, rinsing and sterilization. The method has the advantages of being high in decellularization efficiency, small in damage of matrix components, low in immunogenicity of the decellularization matrix, and good in biocompatibility.

A method for preparing the decellularized matrix using the phospholipase includes the following steps: pretreating the standby tissue and organ; putting the standby tissue and organ into the solution containing the phospholipase; preparing the decellularized matrix in the control condition; washing the prepared decellularized matrix.

The invention discloses an injectable hydrogel and a preparation method thereof. The preparation method comprises the following steps: sequentially performing cell crushing, primary proteolysis, cleaning, degreasing treatment and decellularization treatment on biological tissue so as to obtain a decellularized matrix, and performing freeze-drying on the decellularized matrix for later use; performing secondary proteolysis on the freeze-dried decellularized matrix so as to obtain a 6-40mg/mL water-soluble extracellular matrix protein solution; and crosslinking the extracellular matrix protein solution with 10-100mg/ml multi-arm modified polyethylene glycol containing a succinimide group, and/or a sulfydryl group, and/or a maleimide group and/or an amino group, so as to obtain the njectablehydrogel. The njectable hydrogel is prepared by using the method. The preparation method disclosed by the invention is simple, and the prepared hydrogel not only has the biological inductivity of collagen, but also has the property of controllable degradation rates of high polymer materials.

The invention discloses an artificial blood vessel and a preparation method thereof. Cell components of an autologous-tissue vessel are removed by adopting a 3-[(3-cholamidopropyl)-dimethylammonio]-1-propane sulfonate reagent chemical decellularization method; then heparin is combined to the surface of an autologous decellularized matrix vessel in a covalent binding manner, so as to obtain the artificial blood vessel. The artificial blood vessel prepared by the method has no immunogenicity; a preparation period is short and the cost is low; the artificial blood vessel can be prepared into different inner diameters and has a good anticoagulation function; a complicated in-vitro preparation process is not needed and the patency rate is high; cells can effectively enter a vessel wall to be reconstructed and the artificial blood vessel is completely better than previous allogenic or heterogeneous decellularized matrix vessels and has a very good clinical application prospect.

Decellularized matrix microspheres comprising a polymeric material and a donor tissue are provided. Also disclosed are structures containing a plurality of decellularized matrix microspheres incorporating a first polymer and a donor tissue; and a second polymer, wherein the decellularized matrix microspheres and the second polymer are in the form of a filament. Methods of treating a tissue injury employing the matrix microspheres and structures described as well as their methods of manufacture are also provided.

The present invention provides a 3D tissue material, which adopts porcine small intestine mucosal decellularized matrix as a scaffold material, and is characterized by comprising cells, riboflavin or a derivative thereof. According to the present invention, the chemical components contained in the prepared 3D tissue material are highly close to the human extracellular matrix components, the prepared 3D tissue material is rich in growth factors, has good biocompatibility, and is favorable for cell growth; and the production process is simple, only the incandescent lamp is required to be adopted as the production equipment, the complex equipment is not required, the component harmful to the cell does not exist during the whole operation process, and the whole operation process cannot cause the environmental pollution.

The invention discloses a piezoelectric stent as well as a preparation method and application thereof, and the preparation method of the piezoelectric stent comprises the following steps: performing decellularization treatment on bone tissue organs, and then performing supercritical CO2 extraction to prepare a decellularized matrix with a three-dimensional porous structure; preparing drug-loaded microspheres; then mixing the drug-loaded microspheres with the decellularized matrix so as to enable the drug-loaded microspheres to cover the surface of the decellularized matrix and fill pores of the decellularized matrix, and carrying out heat preservation at 37-65 DEG C until the drug-loaded microspheres are bonded on the decellularized matrix so as to prepare a stent containing the drug-loaded microspheres; and then soaking the stent containing drug-loaded microspheres in a degradable piezoelectric polymer solution, taking out the stent for draining, and carrying out polarization treatment. The stent prepared by the preparation method of the piezoelectric stent can effectively delay the release rate of a drug, has osteogenic differentiation capacity and a piezoelectric response function, and can effectively promote repair and reconstruction of bone tissue.

The invention provides an acellular matrix particle product for soft tissue filling and repairing, and a preparation method of the acellular matrix particle product. The acellular matrix particle product comprises the following components in percentage by mass: 2.0-10% of acellular matrix particles, 0.1-5.0% of suspending aids and the balance of water for injection, wherein the acellular matrix particles are formed by mixing one or more of non-crosslinked acellular matrix particles, low-crosslinked acellular matrix particles and high-crosslinked acellular matrix particles. The decellularized matrix particle product provided by the invention is good in elasticity, soft in texture and high in resilience force, can form decellularized matrix particle products with different degradation gradients, different degradation time and different space structures according to the preparation technologies of the decellularized matrix particle product, is suitable for filling and repairing soft tissues, including dermal superficial tissues, dermal deep tissues, subcutaneous tissues and the like, and has a good application prospect.

The invention provides a low-immunogenic bone defect part filling material. The filling material is composed of aminated aloe polysaccharide-glyceraldehyde gel, heparin high affinity cytokine-entrapped heparin-poloxamer gel and a bone acellular matrix, and the mechanical strength and the degradation speed of the bone defect part filling material are controlled by changing the ratio of the components. The low-immunogenic bone defect part filling material overcomes the shortcomings and deficiencies of high immunogenicity in the prior art, provides a novel bone defect part filling material with low immunogenicity and multiple functions of bone conduction, bone induction, bone formation and mechanics support, provides the best physicochemical and biological micro-environments for bone regeneration, effectively guides the directional arraying of cells, has good mechanical properties, and can quickly repair bone defect parts.

The invention discloses a biological cornea prepared from swimming bladders as well as a preparation method and application of the biological cornea. Fresh swimming bladders are subjected to impurity removal, degreasing, desugaring and freeze thawing treatment, and then a decellularized solution is added to obtain a decellularized matrix of the swimming bladders; and carrying out collagenous fiber reconstruction treatment on the acellular matrix by using an alcohol-water mixed solution containing a light transmission regulator to obtain the artificial biological cornea. The swim bladder is used as a material for preparing the biological cornea, the obtained biological cornea is very close to a natural cornea in component, has excellent optical properties and excellent mechanical properties, shows excellent biocompatibility and is free of cytotoxicity through in-vitro cell culture, and cornea cells can be normally adhered and grow on the surface of the material, so that the biological cornea can be used for preparing the biological cornea. And a compact cell layer is formed and can be used as a medical material in corneal transplantation and corneal lamella repair. The carrier stent is wide in raw material source, low in production cost, convenient to store and use and capable of meeting the requirements of a large number of carrier stents of tissue engineering corneas.

The invention belongs to the technical field of tissue engineering, and particularly relates to injectable bioactive composite conductive hydrogel as well as a preparation method and application thereof, and the composite conductive hydrogel is prepared through respective physical crosslinking of acellular matrix hydrogel and PEDOT: PSS. The composite conductive hydrogel provided by the invention has extremely high electron transmission efficiency and mechanical strength, can well reduce a microenvironment for cell growth, has excellent biocompatibility, can promote tissue regeneration, has good fluidity and injectability, is not limited by shape and size when in use, can well adhere to tissues after gelatinization, and can be used for preparing the hydrogel. Extra surgical suture is not needed; the conductivity of the hydrogel can be adjusted by adjusting the adding amount of PEDOT: PSS, electrical stimulation is provided for cells by externally adding weak current, cell proliferation, migration and functionalization can be effectively promoted, and the hydrogel is expected to be prepared into a biomedical conductive material for application.

The invention relates to the field of tissue engineering and biomedical materials, in particular to a preparation method of biogel and the biogel prepared by the preparation method. The method comprises the following steps: soaking an omentum by using a buffer solution containing lauryl sodium sulfate and DNA enzyme for decellularization treatment; and digesting decellularized omentum tissue by using the combined digestive enzyme liquid in combination with a digestion bottle with a rotor, and preparing the omentum decellularized matrix biogel. According to the invention, the decellularized matrix biogel is prepared by adopting the combined digestive enzyme liquid and a special digestion method thereof. Compared with the existing method, the method disclosed by the invention is milder in condition, and the obtained omentum decellularized matrix biogel can well maintain the content of various active cell factors, has good temperature sensitivity, and can be converted into the gel state from liquid state under the condition of 37 DEG C within 5-10 minutes, so that the hydrogel can be used as an injectable carrier material to be applied to cell therapy of different diseases.

The invention discloses injectable and photo-crosslinked acellular matrix composite hydrogel as well as a preparation method and application thereof. The preparation method comprises the following steps: (1) mixing acellular matrix powder with an acidic pepsin solution, stirring, and removing undigested matrix particles to obtain an acellular matrix pre-gelatinizing solution; (2) carrying out precooling treatment on the decellularized matrix pregelatinizing solution, adding an alkaline solution, and adjusting the pH value to 7.0-7.4; and (3) dissolving polyethylene glycol diacrylate and a photoinitiator in a pre-cooled buffer solution, uniformly stirring with the pre-gelatinizing solution in the step (2) at 0-8 DEG C, and carrying out photo-crosslinking curing to obtain the acellular matrix composite hydrogel. The preparation method is simple and high in operability, and the prepared composite hydrogel is high in mechanical strength, long in degradation time and good in biocompatibility; the hydrogel can be used for loading growth factors, and illumination crosslinking is performed after the hydrogel is injected into a living body, so that sustainable release of the growth factors in the living body is realized, and tissue repair and regeneration are promoted.