108 results about "Decellularized matrix" patented technology

The invention discloses a hydrogel-acellular matrix composite patch containing functionalized extracellular vesicles and a preparation method of the hydrogel-acellular matrix composite patch. The composite patch comprises a hydrogel layer and an acellular pericardium matrix material layer, and an extracellular vesicle-macrophage membrane fusion body (EV-MM) is entrapped in the cross-linked hydrogel. From two aspects of improving the targeting of EVs to the cardiac infarction part and enhancing the retention of the EVs at the cardiac infarction part, the distribution condition of the EVs at the cardiac infarction part is improved, so that the action efficiency of the EVs is improved, and the treatment effect is improved.

The invention discloses a preparation method of an acellular matrix containing a bioactive coating, and belongs to the field of medical beauty industry, the key point of the technical scheme is that the preparation method comprises the preparation steps of S1, preparation of the acellular matrix; s2, treating the acellular matrix; s3, rehydrating the decellularized matrix, sequentially immersing the rehydrated decellularized matrix into an activating solution, a collagen solution and an activating solution, reacting, washing the collagen matrix, and freeze-drying to obtain the collagen matrix; s4, adding the anti-inflammatory microcapsules and the growth factor nano-carrier into the hyaluronic acid solution to obtain a mixed solution; s5, the collagen matrix is immersed in an activating solution, after reaction and washing, the surface of the collagen matrix is coated with the mixed solution for reaction, after curing, washing and drying are conducted, and the acellular matrix containing the bioactive coating is obtained.The covalently-connected hyaluronic acid-collagen double-layer structure is constructed on the surface of the acellular matrix, so that biocompatibility and safety are improved; meanwhile, the problems that a traditional acellular matrix is unstable in structure and poor in mechanical strength are solved.

The invention discloses preparation and application of dual-network hydrogel. The dual-network hydrogel comprises the following components: (Z1) a spinal cord acellular matrix network DSM formed by self-assembly of a spinal cord acellular matrix dSECM; and (Z2) a metal-polyphenol network MPN formed by coordination of metal ions and polyphenols; wherein the DSM and the MPN are cross-linked through hydrogen bonds to jointly form a double-network structure. DSM provides a microenvironment suitable for cell survival and growth for the dual-network hydrogel and induces stem cells to differentiate into neurons, and MPN provides the dual-network hydrogel with biological effects of inhibiting inflammation and accumulation of reactive oxygen species (ROS). The dual-network hydrogel has the advantages of the two components, the active component neurotrophic factor and the inhibitor loaded on the dual-network hydrogel can regulate and control immune response after SCI, improve neuroinflammation microenvironment and promote nerve repair, and a new strategy is provided for in-situ tissue repair of SCI.

The invention relates to composite hydrogel for tissue repair as well as a preparation method and application of the composite hydrogel. The composite hydrogel is prepared from a methacrylated acellular matrix, nano clay (LAP), a photoinitiator, carboxymethyl chitosan (CMCS) and water. The acellular matrix is modified through methylacryloylation, and a polymer network is reinforced by combining nano-clay, so that the mechanical strength is improved; and carboxymethyl chitosan adjusts the degradation rate, constructs a pore structure and endows antibacterial and anti-inflammatory functions. The preparation method comprises the following steps: dissolving the methacrylated acellular matrix in the PBS containing the photoinitiator, dissolving the carboxymethyl chitosan in ultrapure water, mixing, and carrying out illumination cross-linking curing. When the composite hydrogel is applied to small intestine tissue engineering, a small intestine defect part can be filled with the composite hydrogel, photo-crosslinking curing is performed to form a stent, the degradation period is matched with the small intestine regeneration period, and the antibacterial rate is gt; the cell toxicity is 0 level, a good microenvironment can be provided for cells, tissue repair can be promoted, and the compound can also be used for preparing pharmaceutical compositions for treating small intestine related diseases.

The application belongs to the technical field of medical materials, and discloses a soft tissue repair patch and a preparation method thereof, which comprises the following steps: S1, treating and cleaning animal soft tissue to obtain a first intermediate product; S2, adding the first intermediate product into a reagent to perform freeze-thaw circulation to obtain a second intermediate product; S3, soaking and treating the second intermediate product with an organic solvent and cleaning to obtain a third intermediate product; S4, soaking and treating the third intermediate product with an alkali solution and cleaning to obtain a fourth intermediate product; and S5, soaking and treating the fourth intermediate product with an acid solution, cleaning, drying and sterilizing to obtain the soft tissue repair patch. The application provides a more gentle and friendly decellularization scheme, and the decellularized matrix prepared by using the scheme is more completely decellularized, has no chemical residue, and maintains the natural scaffold structure of collagen.

The invention belongs to the technical field of biomedical engineering and tissue engineering, and particularly relates to an acellular matrix modified bionic micro-fluidic organ chip constructed by 3D printing composite near-field direct writing as well as a preparation method and application of the bionic micro-fluidic organ chip. According to the bionic micro-fluidic organ chip prepared by the invention, the microstructure and physiological similarity of a model are remarkably improved, the problems of insufficient bionic property, single cell and the like at present are solved, and the preparation method is simple, is suitable for batch production and solves the problem of high production cost. The invention provides a new technical direction for the construction of the bionic organ model.

Provided is a use of a biological material in preparing a product for limiting gastric volume. The biological material is selected from a decellularized matrix, an artificial extracellular matrix and a high molecule compound. The wrapping of a remaining stomach after gastric volume reduction surgery by the biological material can effectively reduce the expansion of the gastric volume after the surgery for a long time. The gastric volume of the remaining stomach can be effectively maintained, avoiding the occurrence of weight regain caused by the expansion of the remaining stomach. The decellularized matrix and a biological material remolded tissue have the viscoelasticity equivalent to or close to natural tissue, avoiding gastric wall stiffness, and thus not affecting gastric wall peristalsis.

The invention relates to the technical field of biomedical engineering, in particular to a bile duct for screening and developing drugs or simulating bile duct cancer fibrosis progress in vitro. The bile duct is prepared by the following steps: (1) treating a liver by using a lauryl sodium sulfate solution and a Triton X-100 solution in sequence, freeze-drying and crushing; (2) carrying out enzymolysis on the crushed tissues by using pepsin, and filtering to obtain acellular matrix hydrogel; and (3) mixing the acellular matrix hydrogel with a sodium alginate solution to obtain the composite hydrogel. The structure of the sodium alginate-acellular matrix composite hydrogel can be accurately controlled by adopting a 3D printing technology. Therefore, the hydrogel can better simulate a tumor microenvironment, and particularly can realize more real biocompatibility and tissue structure when being used for culturing bile duct cancer organs.

By means of the method according to one aspect, in a process of enhancing biocompatibility of an acellular matrix by supercritical carbon dioxide treatment, immunogenicity of the acellular matrix can be reduced with effective efficiency while maintaining a content of a physiologically active substance and physiologically active ability. Accordingly, the acellular matrix prepared by the method according to one aspect, the composition including the same, and the biomaterial have low biotoxicity and excellent tissue regeneration energy, and thus can be usefully used in cosmetic or medical materials.

The present invention relates to the field of medical material technology, and in particular to a decellularized matrix tubular material and a preparation method thereof. The preparation method includes the preparation of a dense decellularized matrix tissue fiber aggregate; the preparation of a decellularized matrix tissue layer; and the preparation of a decellularized matrix tubular material. The decellularized matrix tissue layer is repeatedly rolled up using a mold through a rolling technique, so that the micro-nano fibers are interwoven, wrapped, and tightly bonded together to form a decellularized matrix tubular material composed of a single layer or several layers of single-layer decellularized matrix tissue layers. The decellularized tubular scaffold material prepared by the present invention has stable and tight bonding between the layers, is easy to operate, has controllable pore size and porosity of the tube wall, and can be prepared into a single-cavity, multi-cavity or multi-branched tubular scaffold, overcoming the shortcomings of existing tubular decellularized matrix materials, such as dense structure, the need for suturing or bonding, and difficulty in preparing branched structures.

The invention provides a tissue engineering heart valve stent with a sulfonated nano-enzyme coating as well as a preparation method and application of the tissue engineering heart valve stent, and belongs to the technical field of medical materials. The invention relates to a tissue engineering heart valve stent with a sulfonated nano-enzyme coating, which comprises an acellular matrix carrier, ruthenium / iridium-tannic acid nano-enzyme loaded on the acellular matrix carrier, and a sulfonated polymer coating fixed on the acellular matrix carrier and / or the surface of nano-particles through ultraviolet polymerization, the ruthenium / iridium-tannic acid nano-enzyme is formed by tannic acid TA, Ru < 3 + > and Ir < 3 + > through coordination. According to the invention, through double bionic activities of superoxide dismutase and nitric oxide synthase of ruthenium / iridium-tannic acid nano-enzyme and through the super-hydrophilicity and electrically neutral surface of the sulfonated polymer coating, collaborative regulation and control of O2-NO cascade catalysis and interface functions in a pathological microenvironment are realized; the structural and functional in-situ regeneration of the tissue engineering heart valve stent is promoted.

The invention provides acellular matrix hydrogel loaded with animal acellular omentum particles as well as a preparation method and application of the acellular matrix hydrogel loaded with the animal acellular omentum particles, and relates to the technical field of tissue engineering and regenerative medicine. Comprising a decellularization treatment agent, a decellularization matrix, functional particles, an enzymolysis agent, a cross-linking stabilizer, a functional additive and a solvent, the functional additive is prepared from PDA coated Fe3O4 magnetic nano particles, phosphorylated polyglycerol sebacate and liposome coated silver nano particles. The PDA-Fe3O4 magnetic nanoparticles of the functional additive generate mechanical signals under the action of an external magnetic field, a Piezo1 mechanical gating cation channel is activated to promote calcium ion internal flow, and the autonomous mineralization ability of phosphorylated polyglycerol sebacate is combined, so that the activity of the bone differentiation marker alkaline phosphatase is effectively improved, and the bone differentiation marker alkaline phosphatase is prepared. The combination of the PDA coated Fe3O4 magnetic nanoparticles and the phosphorylated polyglycerol sebacate integrates a magnetic response mineralization system.

The invention discloses an antibacterial composite periosteum for an oral cavity and a processing technology of the antibacterial composite periosteum, and relates to the technical field of periosteum. The method specifically comprises the following steps: step 1, treating defatted pig peritoneum with a decellularized solution, cleaning and sterilizing to obtain a pig peritoneum decellularized matrix; 2, treating the pig peritoneal decellularized matrix with a glyoxal solution, cleaning, and sterilizing to obtain a pretreated pig peritoneal decellularized matrix; step 3, uniformly coating the surface of the pretreated porcine peritoneal acellular matrix with an antibacterial solution, and performing sterilization, standing and freeze-drying to obtain the antibacterial composite periosteum. Wherein the antibacterial solution is prepared from the following raw material components in concentration: 1 to 2 weight percent of modified chitosan, 0.2 to 0.4 weight percent of amino-terminated polyethylene glycol and 0.01 to 0.05 weight percent of p-toluenesulfonic acid, and a solvent is a PBS (Phosphate Buffer Solution). The antibacterial composite periosteum is good in biocompatibility and has excellent clinical application value in oral surgery.

This invention relates to the field of biomedical materials technology, specifically to a decellularized matrix microparticle (dECM-MPs) 3D printing ink and its preparation method. The key technical points are: a heat-absorbing compound is temporarily dissolved on the surface of decellularized matrix microparticles (dECM-MPs) via recrystallization from a supersaturated solution; then, the microparticles are immersed in a temperature-sensitive gelatin solution. The heat absorbed during the dissolution of the heat-absorbing compound leads to a localized low temperature around the microspheres, causing the gelatin to solidify on the dECM-MPs surface, forming a physically cured gelatin hydrogel microlayer in situ. This microlayer is further stabilized by enzymatic cross-linking. Photosensitive modified hyaluronic acid molecules are introduced into the hydrogel microlayer to regulate its mechanical properties. Centrifugation yields a particulate ink suitable for extrusion 3D printing. After 3D printing, ultraviolet light irradiation is used to produce a 3D printed implant. This invention overcomes the problem that rigid dECM-MPs, such as decellularized cartilage microparticles or decellularized bone microparticles, cannot be extruded and printed. By regulating the mechanical properties of the hydrogel microlayer, particulate ink with excellent printing performance can be obtained. This method overcomes the limitation of rigid tissues being difficult to additively manufacture.

The application discloses a preparation method of a decellularized matrix and a product prepared by the method. The preparation method comprises the following steps: (1) washing a mammalian soft tissue to remove blood, lipids and impurities, and cutting the tissue into pieces with a thickness of 1-10 mm; (2) soaking the pieces in solution 1 for 6-48 h, and then soaking the pieces in solution 2 for 0.1-1 h; (3) treating the pieces in an ultrasonic water bath under the condition of a frequency of 20-40 kHz for 5-30 min; (4) water-bathing the pieces at 55-65 DEG C for 10-30 min; (5) sequentially washing the tissue with deionized water, a PBS buffer and ethanol, and washing the tissue with each solution for 1-3 times, each time for 10-30 min; and (6) freeze-drying the decellularized matrix material, and crushing the material at-80 DEG C to-20 DEG C to obtain a decellularized matrix powder. The preparation method significantly improves the cell component removal efficiency, effectively protects structural proteins such as collagen from being degraded in the freeze-drying and crushing processes, and ensures the integrity of the matrix in the aspects of morphology, structure and function, thereby providing a structural support and biological signal support for subsequent tissue repair.

The invention relates to an acellular matrix repair material as well as a preparation method and application thereof. The preparation method of the acellular matrix repair material comprises the following steps: providing an acellular matrix of a peritoneal tissue of a mammal, and cross-linking the acellular matrix with a cross-linking agent to prepare a cross-linked acellular matrix; preparing an antibacterial drug into nano drug-loaded particles, and connecting the nano drug-loaded particles with the cross-linked acellular matrix to prepare a drug-loaded acellular matrix; and carrying out freeze-drying treatment on the drug-loaded acellular matrix to prepare the acellular matrix repair material. The invention provides the preparation method of the acellular matrix repair material, the obtained repair material can be used for repairing urethral defects, the excellent degradation resistance of the repair material can prolong the degradation rate of the material in vivo, effectively prevent contracture and reduce the recurrence rate of urethrostenosis, and the good antibacterial ability can enhance the anti-infection ability, so that the repair material can be used for repairing urethral defects. And the risk of urethral infection of postoperative patients is reduced.

The present invention provides a method for preparing a decellularized adipose tissue matrix. Specifically, it provides a method for rapidly and efficiently preparing a decellularized adipose tissue matrix using only physical and chemical methods, without the introduction of bio-enzymes, to remove cell nuclei and lipids from allogeneic adipose tissue while preserving the matrix's natural structure. The resulting adipose tissue matrix exhibits high biosafety and contains a variety of natural bioactive components, such as collagen and proteins. It can be used in fields such as filling soft tissue volume loss caused by trauma, disease, and aging, as well as in tissue regeneration.

The invention relates to the technical field of artificial dermis, in particular to a preparation method and application of artificial dermis based on bionic acellular matrix. The artificial dermis comprises a bionic acellular matrix component A and an auxiliary material component B, wherein the component A comprises type I collagen, type VI collagen, elastin, fibronectin, laminin, mother cell protein and glucan; the component B comprises antibacterial peptide, antibiotics and a high-molecular polymer material; the mass ratio of the component A to the component B is (4-6): (1-4). The preparation method comprises the following steps: respectively preparing procedure mother liquor of the component A and the component B, preparing hydrogel with a double-layer gradient structure through step-by-step crosslinking, and then carrying out freeze drying and sterilization treatment. The artificial dermis disclosed by the invention has good biocompatibility and antibacterial property, can effectively promote wound healing, is suitable for repairing acute and chronic wounds of four limbs, faces and trunk parts, and is particularly suitable for repairing complex wounds with exposed bones, tendons, nerves and blood vessels.

The application discloses an artificial blood vessel stent with anticoagulation performance and a construction method thereof. After a pig heart aorta blood vessel is subjected to decellularization treatment, a pre-gel solution is prepared by compounding polyvinyl alcohol and methacrylated gelatin, a designed model is printed into a hollow tubular mold by using 3D printing, the pre-gel solution is injected into the mold to obtain an artificial blood vessel stent with controllable shape and size after cross-linking, and finally, the artificial blood vessel stent loaded with heparin is obtained by loading the heparin on the prepared stent. The material for preparing the pre-gel solution is selected, and the advantages of the material functions can be complemented. The decellularized matrix is beneficial to cell adhesion, proliferation and differentiation; the compounding of polyvinyl alcohol and methacrylated gelatin improves the mechanical properties of the stent and improves the cell adhesion. The heparin is loaded on the artificial blood vessel stent to improve the anticoagulation performance of the stent and prevent the formation of thrombus. The stent serves as an excellent biomedical material and has a wide application prospect in the fields of cardiovascular tissue engineering and biology.

The present invention provides a decellularized matrix microparticle freeze-dried implant, a preparation method and an application thereof, belonging to the technical field of soft tissue filling materials. The decellularized matrix microparticle freeze-dried implant is composed of a decellularized matrix, a suspending agent and a freeze-drying protectant, wherein the mass ratio of the decellularized matrix, the suspending agent and the freeze-drying protectant is 0.5-10:0.5-10:0.5-6; the particle size of the cell matrix freeze-dried implant is 10 μm-1000 μm. The decellularized matrix microparticle freeze-dried implant of the present invention retains the natural three-dimensional structure and natural active ingredients, reduces the immune rejection reaction caused by the raw material source after the injection product is clinically used, and is suitable for injection into irregular defects for filling and repair, injection into the deep subcutaneous dermis to the superficial subcutaneous layer to repair moderate to severe wrinkles or folds, and has a good repair effect.

The application discloses a composite hydrogel based on urethral stem cell membrane sheet ECM and exosomes and a preparation method and application thereof, and relates to the technical field of tissue engineering, and in particular relates to a soluble ECM protein solution obtained by dissolving and enzymatically hydrolyzing urethral stem cell membrane sheet decellularized matrix (ECM) powder and mixing the soluble ECM protein solution with exosomes in a mass ratio of 3:1-10:1, and performing temperature-sensitive crosslinking in DMEM at pH 7.0 and 36-38 DEG C to form a hydrogel. The composite hydrogel based on urethral stem cell membrane sheet decellularized matrix (ECM) and exosomes integrates the cell activity of the urethral stem cell membrane sheet, the structural support of the decellularized matrix (ECM) and the signal regulation of the exosomes, constructs a three-level functional system, forms a synergistic repair effect, and is helpful to promoting angiogenesis in the urethra. Through covalent coupling of the decellularized matrix (ECM) fiber network and the surface protein of the exosomes, the intelligent release of the exosomes in an inflammatory environment is realized, and the composite hydrogel is used for urethral repair and angiogenesis promotion.

The present application belongs to the field of biomedical materials and tissue engineering, and particularly relates to a kind of acellular matrix aerogel repair material and its preparation method and application.Further, the present application relates to the comprehensive preparation method of the material, especially the extremely low immunogenicity acellular process combined with physical, chemical and supercritical fluid technologies, and the unique aerogel forming technology based on supercritical drying.Further, the present application relates to the application of the material, mainly related to soft tissue repair, medical cosmetology, bone / cartilage injury repair field.

The invention discloses application of an adipose acellular matrix prepared on the basis of adipose tissue in the aspect of drug sustained release. The fat acellular matrix is prepared by performing cleaning, peroxyacetic acid virus inactivation, supercritical carbon dioxide decellularization and degreasing treatment on adipose tissues, and splitting the adipose tissues into particles with proper particle sizes. The material can be used as a drug sustained-release carrier material, has good biocompatibility, excellent drug loading and sustained-release performance and degradability, and can be widely applied to sustained release of various drugs including antibiotics, antitumor drugs and the like. The invention provides a novel and potential material source and a preparation method for the field of drug sustained release.

The application relates to the field of biomaterials, and particularly discloses a kind of acellular matrix material and a preparation method thereof.The preparation method of the material comprises the following steps: extracting tissue material of a mammal, performing acellular treatment on the tissue material to obtain acellular tissue material, freeze-drying the tissue material, and then crushing the freeze-dried tissue material to obtain animal-derived acellular matrix; the animal-derived acellular matrix is resuspended in water, mixed with a modified epoxidized chitosan solution, and then transglutaminase is added to perform a reaction; the product obtained is freeze-dried to obtain the acellular matrix material.The method can improve the biocompatibility of the acellular matrix and reduce the immune rejection reaction by cross-linking the animal-derived acellular matrix with modified epoxidized chitosan; at the same time, since the cross-linking reaction is a biological cross-linking reaction under the action of transglutaminase, the biological activity factors contained in the acellular matrix are not easily destroyed during the cross-linking process, and the biological activity and safety are better.

The application provides an injectable hydrogel for endometrial repair and / or prevention of adhesion of uterine cavity and a preparation method and application thereof, and belongs to the technical field of biomedical materials. The injectable hydrogel is composed of a base gel and a decellularized matrix distributed in the base gel; the base gel is composed of a cross-linked carboxymethyl chitosan gel and a carbomer gel; the base gel has an interpenetrating or semi-interpenetrating double network structure; and the cross-linked carboxymethyl chitosan gel is a chemical cross-linked carboxymethyl chitosan gel. In the application, the injectable hydrogel forms a base gel with an interpenetrating or semi-interpenetrating double network structure by using a cross-linked carboxymethyl chitosan gel and a carbomer gel, and is compounded with a decellularized matrix, so that the injectable hydrogel has high elasticity and high adhesion, can effectively prevent adhesion and repair damaged tissues, has a long action time, and has good biocompatibility.

This invention relates to a decellularized oral collagen plug and its preparation method. The plug comprises an upper decellularized matrix membrane and a lower decellularized sponge plug. The preparation method is as follows: pretreated fresh animal-derived membrane material is treated with trypsin solution and sodium hydroxide solution, washed with purified water to obtain a decellularized matrix membrane; a portion of the obtained decellularized matrix membrane is placed in a low-temperature tissue ball mill, pulverized, homogenized, filtered, and washed with purified water to obtain a decellularized collagen solution; the decellularized collagen solution is poured into a freeze-drying mold, defoamed under low-temperature vacuum, covered with the decellularized matrix membrane, and after vacuum defoaming, freeze-dried and vacuum thermally cross-linked to obtain the finished collagen plug. This invention's decellularized oral collagen plug is suitable for filling and covering wounds after tooth extraction. The upper decellularized matrix membrane effectively prevents fibroblasts and epithelial cells from invading bone defect areas, while the lower decellularized sponge plug stabilizes blood clots, provides a biological scaffold, reduces postoperative complications, and promotes wound healing.

This invention discloses a biomimetic decellularized matrix hydrogel and its applications. It relates to the field of biomaterials technology. It comprises the following components: decellularized liver matrix, gelatin, and sodium alginate, and its applications are provided. The therapeutic artificial liver provided by this invention can be mass-produced in vitro using 3D bioprinting technology, exhibiting mature liver functional phenotypes such as ICG uptake / release and drug metabolism in vitro. It was transplanted into a type I tyrosinemia model of liver failure (Fah). ‑ / ‑ It can alleviate liver damage in mice and has the potential for artificial liver transplantation.

The invention provides a preparation method of a collagen-based bone filling material, which comprises the following steps: S1) heating an acellular matrix biological membrane for thermal crosslinking treatment, and crushing to obtain matrix powder; and S2) mixing the matrix powder with a solvent, performing whipping treatment, then adding inorganic bone particles, performing mixing, and performing freeze drying and thermal crosslinking treatment to obtain the collagen-based bone filling material. Compared with the prior art, the collagen-based bone filling material provided by the invention is composed of the acellular matrix biological membrane and the natural inorganic bone particles, and the acellular matrix biological membrane is subjected to thermal crosslinking treatment, crushing, swelling and whipping treatment to form a stable three-dimensional network structure; the bone filling material prepared by the preparation method disclosed by the invention can obviously prolong the in-vitro degradation time after being physically crosslinked with inorganic bone particles, and the bone filling material has no chemical reagent residue, not only has good biocompatibility, but also has good rebound resilience and plasticity, and is convenient to operate.

This application relates to a decellularized scaffold for cartilage repair in the biomedical field, its preparation method, and application. The preparation method comprises: preparing a decellularized matrix from animal cartilage; chemically bonding the decellularized matrix with a lipid compound to obtain a double-bond-modified decellularized matrix; copolymerizing and cross-linking the double-bond-modified decellularized matrix to obtain a decellularized matrix hydrogel; and immersing the decellularized matrix hydrogel in a dipeptide solution for modification to obtain the decellularized scaffold. The decellularized scaffold of this application introduces both mechanical and electrical stimulation effects into the scaffold, addressing deficiencies in previous research and demonstrating good biocompatibility and repair effectiveness in both in vivo and in vitro experiments.

Methods and compositions of matter for the treatment of multiple organ dysfunction syndrome using products derived from autologous pluripotent derived stem cells. Autologous pluripotent stem cells can be induced to differentiate into therapeutic cell populations including mesenchymal stem cell and regulatory T cell populations in the presence of a decellularized matrix. Said cells can be transfected with therapeutic genes to enhance function and activity upon administration to a subject. Combinations of autologous cell types can be utilized. In such combinations, the cell populations are conditioned to regulate disease and modulate immune responses for the treatment of multiorgan failure.