The invention discloses a self-sucking biological 3D printing nozzle device and a control method thereof, and belongs to the field of tissue engineering and biological 3D printing. The nozzle device is characterized by comprising a nozzle, a plunger cylinder, screws, an inclined disc cover, a motor base, a motor, a coupling, a transmission shaft, a flat key and a flow distribution cylinder. The motor drives the transmission shaft to rotate through the coupling to drive the plunger cylinder coaxially mounted in the flow distribution cylinder to rotate, so that a plunger body on the plunger cylinder moves up and down in a cylinder plunger hole under the combined effect of spring force and an inner inclined surface of the inclined disc cover to realize continuous sucking and pressing actions to finish continuous biological material printing work. The nozzle device effectively prevents the trouble of the need of stopping to replace the nozzle device when being applied to continuous printing of large-size tissue material organs. The nozzle device has the advantages of simple and skillful structure concept, easy realization of automatic control, improvement of the printing efficiency and effective solving of the continuous supply printing problem.