13results about How to "Low antigenicity" patented technology

The invention discloses an ultrasonic molecular probe. The ultrasonic molecular probe comprises a targeted contrast agent and siRNA of fibronectin, wherein the targeted contrast agent and the siRNA are connected through G0-C14; the targeted contrast agent comprises gas vesicles covalently linked with annular polypeptide, and the annular polypeptide targets an atherosclerotic plaque region. The cyclic polypeptide can be combined with the outer structural domain B of fibronectin with high specificity, and the atherosclerotic plaque extracellular matrix is rich in the outer structural domain B of the fibronectin, so that enough targeted ultrasonic contrast agent can be quickly combined in the plaque region to generate strong signal enhancement in ultrasonic imaging, and on the basis, the targeted ultrasonic contrast agent can be quickly combined with the plaque region to generate strong signal enhancement in ultrasonic imaging. The ultrasonic molecular probe releases siRNA for atherosclerotic plaque treatment, and atherosclerotic plaque diagnosis and treatment integration is achieved. The invention further discloses a preparation method of the ultrasonic molecular probe and application of the ultrasonic molecular probe in preparation of atherosclerotic plaque diagnostic agents, drugs and drug delivery agents.

The invention provides a preparation method of a mineralized collagen scaffold material with a bionic structure, and belongs to the field of biomedical engineering.The preparation method comprises the steps that an amorphous calcium phosphate solution stabilized by polyacrylic acid is prepared, and the molar ratio of Ca to P in the amorphous calcium phosphate solution is 1.67: 1; preparing a collagen solution containing noradrenaline; carrying out isoelectric focusing treatment on the collagen solution under a constant voltage to form a dense membrane, and stretching the dense membrane to generate 120-200% deformation, so as to obtain a collagen membrane with a bionic structure; and soaking the collagen membrane in the amorphous calcium phosphate solution, and then performing freeze drying to obtain the noradrenaline-mineralized collagen scaffold material. The mineralized collagen scaffold material prepared by the method has excellent biocompatibility, osteogenesis inductivity and sufficient mechanical strength, can effectively promote bone tissue defect repair, and has a good application prospect in the field of bone tissue engineering. The invention further provides the mineralized collagen scaffold material with the bionic structure and application of the mineralized collagen scaffold material.

The invention discloses an enzyme-labeled secondary antibody polymer for immunohistochemical staining. The enzyme-labeled secondary antibody polymer is formed by combining a secondary antibody and a polymer skeleton, the polymer is labeled by a labeling enzyme, the polymer skeleton is polymethylacrylic acid-2-hydroxyethyl ester, and the labeling enzyme is connected to the polymethylacrylic acid-2-hydroxyethyl ester through a coupling agent. According to the invention, polymethylacrylic acid-2-hydroxyethyl ester is used for modifying protein, so that the immunogenicity and antigenicity are reduced, the hydrolysis of protease can be weakened, and the solubility of protein molecules is improved. The enzyme-labeled secondary antibody polymer is applied to immunohistochemical staining and has high specificity and sensitivity.

The invention belongs to the technical field of microorganisms, and particularly discloses a strain of lactobacillus plantarum and application thereof, and the preservation number of the lactobacillus plantarum SN21 is CCTCC (China Center For Type Culture Collection) NO: M20252076. The lactobacillus plantarum SN21 provided by the invention is applied to fermentation of the peanut protein, the antigenicity of the peanut protein can be effectively reduced by optimizing fermentation conditions, and the oxidation resistance of a product is improved; meanwhile, the lactobacillus plantarum SN21 provided by the invention is used for fermenting peanut protein, and the solubility and the emulsifying property of the lactobacillus plantarum SN21 are improved. The strain has a good application prospect in development and utilization of fermented foods, and particularly has a relatively good application value in development of low-sensitivity peanut products and improvement of functional characteristics.

The invention relates to the technical field of medical instruments, discloses application of VEGF (vascular endothelial growth factor) mimic peptide in a small-diameter intravascular stent, and particularly designs a fast endothelialization SDVG prototype stent adopting a QKCMP (quick K chemical mechanical polishing) technology. The QKCMP technology shows high affinity to the synthetic stent by negatively adjusting a Hippo-YAP / TAZ signal channel, so that the stability and removal resistance of the stent are ensured; in addition, the binding force of the stent is remarkably enhanced by using the VEGF mimic peptide QKCMP, and continuous and effective release of the VEGF is realized; the innovative technology is expected to obviously improve the effect of the small-diameter intravascular stent in clinical application.

The invention discloses a low-sensitivity soybean yogurt prepared by enzymolysis and synergistic bacterial fermentation and a preparation method thereof, belongs to the technical field of bean food processing, and provides a method for preparing low-sensitivity soybean yogurt by enzymolysis and synergistic bacterial fermentation, comprising: soaking soybeans in a sodium carbonate solution, then washing, preheating in a boiling water bath, mixing with water and grinding, ripening treatment, filtering, and obtaining cooked soybean milk; mixing with a flavor protease enzyme solution, enzymolysis, ice water bath to room temperature, and obtaining soybean milk enzymolysis solution; mixing with a glucose solution to obtain a fermentation substrate, inoculating plant lactobacillus, fermenting, and obtaining a fermentation product; post-ripening, and obtaining low-sensitivity soybean yogurt. The invention adopts flavor protease enzymolysis and synergistic bacterial fermentation to prepare soybean yogurt, and the obtained soybean yogurt has a reduced gel size, a delicate microstructure, a weakened syneresis shrinkage effect, and a significantly reduced allergenicity, and a scientific processing technology, a simple method, and easy operation.

The invention discloses a method for preparing low-sensitization protein peptide through microwave combined enzymolysis composite modification and application, and relates to the technical field of food processing. The treatment method comprises the following steps: preparing protein powder into a solution, adding tea polyphenol into the solution, and uniformly mixing to prepare a protein solution; carrying out microwave modification treatment on the protein solution; adding compound protease into the protein solution subjected to microwave treatment for enzymolysis and enzyme deactivation; and filtering the enzymolysis product, and drying the filtrate to obtain the low-sensitization protein peptide. According to the method, microwave and enzymolysis combined composite modification is adopted, so that the sensitization of protein can be reduced, the reaction time is shortened, and the method is simple in process, free of pollution and high in safety. In the enzymolysis process, active peptide substances which are easy to digest and absorb, can enhance immunity and delay senescence are synchronously generated, and tea polyphenol added in the formula can synergistically relieve anaphylactic reaction and play an anti-oxidation role, so that multi-element synergy of desensitization, function enhancement and flavor optimization is realized, and the added value and market application potential of the product are improved.

The invention provides medical-grade tissue-like natural collagen as well as a preparation method and application thereof, and relates to a biomedical material. The invention provides a preparation method of medical grade tissue-like natural collagen, which comprises the following steps: removing subcutaneous tissue of animal skin, soaking in normal saline, sequentially carrying out degreasing treatment, unhairing treatment, swelling removal treatment, acid swelling treatment, ball milling, pH value adjustment to 7-7.4, solid-liquid separation, washing the obtained solid component with water, and drying to obtain the medical grade tissue-like natural collagen. The medical-grade tissue-like natural collagen is obtained. The medical grade tissue-like natural collagen prepared by the invention has good biocompatibility, proper biodegradability, good mechanical properties and excellent thermal stability and structural stability, is low in antigenicity and easy to preserve, can quickly and effectively stop bleeding and promote wound healing and repair, and has a wide application prospect. The material is an ideal material for biomedical materials.

The invention provides a tissue-like natural collagen composite absorbable suture line as well as a preparation method and application thereof, and relates to a biomedical material. The invention provides a tissue-like natural collagen composite absorbable suture line. The tissue-like natural collagen composite absorbable suture line comprises medical-grade tissue-like natural collagen, epoxidized modified chitosan, molybdenum dioxide nanoparticles and sodium alginate. The tissue-like natural collagen composite absorbable suture line provided by the invention fully combines the advantages and functions of the used components, retains the excellent mechanical properties and biodegradability of the natural collagen composite material, is low in antigenicity and excellent in antibacterial property, also combines the photo-thermal effect of molybdenum disulfide, and has an obvious scar elimination effect; meanwhile, corrosion of bile, urine and pancreatic juice can be resisted, and cell growth is promoted; the reutilization of natural biological tissues is realized, and excellent use strength, antibacterial property and scar-removing property are achieved; the raw materials are wide in source and low in cost, and have great advantages in the field of medical suture materials.

This utility model relates to the field of food processing technology and discloses a magnetic field treatment device for improving protein emulsification properties and reducing protein allergenicity. The device includes: a sample bottle, a circulating water pump, and a magnetic field generating mechanism connected in a sequential cycle. The sample bottle has an inlet pipe and an outlet pipe. The circulating water pump is connected to the sample bottle through the inlet pipe, and the magnetic field generating mechanism is connected to the sample bottle through the outlet pipe. This utility model, by setting up a sample bottle, a circulating water pump, and a magnetic field generating mechanism connected in a sequential cycle, simultaneously achieves the circulating transport of protein liquid and uniformly treats the protein liquid with a magnetic field to generate Lorentz force. It is simple and practical, and can effectively improve protein emulsification properties and reduce protein allergenicity.

The invention belongs to the technical field of functional dressings, and discloses a liquid dressing for skin repair as well as a preparation method and application thereof, the liquid dressing for skin repair is prepared from the following raw materials in percentage by weight: 1-5% of zinc hyaluronate, 2-10% of giant salamander collagen, 1-8% of aloe polysaccharide, 0.5-6% of L-lactic acid, 0.5-2% of carbomer, 0.1-0.5% of preservative, 0.1-2% of pH regulator and the balance of purified water. The components of the dressing are reasonably proportioned and synergistically gained, and the dressing has the effects of resisting bacteria, resisting oxidation, promoting angiogenesis and the like, can effectively promote skin wound repair, and also has good safety and use comfort.

The purpose of the present invention is to provide a fiber sheet structure comprising a doubled-twisted tow comprising a gelatin fiber bundle and a thermoplastic resin fiber bundle, the gelatin fiber bundle having a better texture than conventional gelatin fibers and having a high tensile strength and a large elongation at break. Furthermore, the purpose of the present invention is to provide a fiber sheet structure which comprises a doubled-twisted tow comprising a gelatin fiber bundle and a thermoplastic resin fiber bundle, has excellent cell adhesion for fixing cells, and has sliding properties such that inflammation does not easily occur when the fiber sheet structure rubs against biological tissue. A fiber sheet structure having a cell placement surface and a back surface, the back surface having a smaller average pore diameter than the cell placement surface, the fiber sheet structure including a knitted structure including a doubled tow comprising gelatin fiber bundles and thermoplastic resin fiber bundles, the thickness of the fiber sheet structure is 30-5000 [mu] m, the number of gelatin fibers constituting the gelatin fiber bundles is 5-60, the fineness of the gelatin fibers is 0.5-10 dtex, the total number of the gelatin fiber bundles and the thermoplastic resin fiber bundles in the doubled tow is 2-20, and the ratio of the number of the gelatin fiber bundles and the number of the thermoplastic resin fiber bundles in the doubled tow is 5: 1-1: 5.

The invention discloses hypoallergenic soybean yoghurt prepared through enzymolysis synergistic bacterium fermentation and a preparation method of the hypoallergenic soybean yoghurt, belongs to the technical field of bean food processing, and provides a method for preparing the hypoallergenic soybean yoghurt through enzymolysis synergistic bacterium fermentation, which comprises the following steps: soaking soybeans in a sodium carbonate solution, then washing, preheating in a boiling water bath, mixing with water, grinding into thick liquid, curing, and filtering to obtain the hypoallergenic soybean yoghurt. Cooked soybean milk is obtained; mixing with flavourzyme liquid, performing enzymolysis, and performing ice-water bath to normal temperature to obtain soybean milk enzymatic hydrolysate; mixing with a glucose solution to obtain a fermentation substrate, inoculating plant lactobacillus, and fermenting to obtain a fermentation product; and after-ripening to obtain the hypoallergenic soybean yoghurt. The soybean yoghurt is prepared by adopting flavourzyme enzymolysis and bacterium fermentation, the gel size of the obtained soybean yoghurt is reduced, the microstructure is fine and smooth, the dehydration shrinkage effect is weakened, meanwhile, the sensitization is remarkably reduced, and the processing technology is scientific in process, simple in method and easy to operate.