341 results about "Cell adhesion" patented technology

The invention provides recombinant IV type collagen and application thereof, and relates to the technical field of protein engineering. In the invention, an amino acid residue repetitive unit with good biological activity and stability in the human-derived IV-type collagen is found, and the amino acid residue repetitive unit is used as a core for repeating and constructing the recombinant IV-type collagen, so that the novel human-derived recombinant IV-type collagen with biological activity is obtained. The human-derived recombinant IV-type collagen provided by the invention is a small-molecular-weight protein, is easy to absorb by a human body, is good in safety, can remarkably promote secretion of collagen, also has the effects of promoting cell adhesion and cell migration, and has a good application prospect in the aspects of basement membrane repair and remodeling. A new functional component is provided for products for repairing and tightening the skin, resisting aging, resisting wrinkles, preserving moisture and the like.

The invention discloses recombinant IV-type collagen as well as a preparation method and application thereof, and belongs to the technical field of bioengineering. The amino acid sequence of the recombinant IV type collagen comprises a plurality of repetitive units, and the repetitive units are shown as SEQ ID NO. 1. The recombinant IV type collagen fragment is derived from human IV type collagen, heterologous amino acid residues are not introduced, and rejection and sensitization risks are avoided; meanwhile, the hydrogel also has good biological activity, including cell proliferation promoting activity and cell adhesion promoting activity, and is good in biocompatibility. In addition, the preparation method of the recombinant IV-type collagen is simple and easy to operate, and the recombinant IV-type collagen with high purity and stability can be obtained through the fermentation and purification method. Therefore, the recombinant IV type collagen disclosed by the invention has a relatively good application prospect.

The invention discloses starch-collagen composite hydrogel as well as a preparation method and application thereof. Collagen is catalyzed by microbial transglutaminase to form a covalent cross-linked network, and meanwhile, hydroxypropyl starch is introduced to construct a physical interpenetrating network through molecular chain penetration and hydrogen-bond interaction; and chondroitin sulfate is further integrated to strengthen the network structure and biological activity through electrostatic interaction. The method is mild in condition and does not need a toxic chemical cross-linking agent, the obtained composite hydrogel has an interpenetrating double-network structure and has excellent mechanical properties, enzymatic degradation resistance and cell affinity, the compression modulus of the composite hydrogel is remarkably improved, the composite hydrogel can keep structural stability for a long time in a collagenase environment, cell adhesion and proliferation can be effectively promoted, and the composite hydrogel has a good application prospect. The hydrogel can be widely applied to tissue engineering scaffolds, wound dressings, drug sustained-release carriers and cartilage repair materials.

The invention relates to the technical field of biological materials, in particular to a pH response collagen microneedle patch loaded with antibacterial nanoparticles as well as a preparation method and application of the pH response collagen microneedle patch loaded with the antibacterial nanoparticles, and the microneedle patch is prepared by loading the antibacterial nanoparticles, aldehyde modified F127 and recombinant three-type collagen COL3 on a polymer matrix. When the microneedle patch is applied to a chronic wound of a diabetic patient, the microneedle penetrates through the skin in a minimally invasive mode and directly reaches a wound focus area. Under the irradiation of 808nm near-infrared light, the mesoporous polydopamine absorbs light energy, so that the loaded lyase LysAB2 is quickly released, bacterial cell walls are specifically recognized and cracked, and bacteria are efficiently killed; meanwhile, the collagen hydrogel constructs an appropriate microenvironment at the wound, cell adhesion, proliferation and migration are promoted, and the wound healing process is accelerated. Compared with a traditional diabetes mellitus chronic bacterial infection wound treatment means, the microneedle patch provided by the invention provides an efficient and safe new strategy for treating the chronic bacterial infection wound of a diabetes mellitus patient.

The invention relates to the technical field of synthetic biology, in particular to polypeptide with a triple helix structure, recombinant XII type humanized collagen and application of the recombinant XII type humanized collagen. The recombinant XII type humanized collagen is successfully expressed and prepared, has a triple-helix structure and good biological activity including promotion of cell proliferation activity, cell adhesion activity and inhibition of MMP-1, is used as a biological material derived from a human body, does not generate immunological rejection and anaphylactic reaction when applied to the human body, and has a good application prospect. The composition can be used for medical or non-medical application of a plurality of tissues and organs of a human body, filling, compatibilizing or repairing, and promotion of skin compactness or wrinkle resistance and other scenes.

The invention belongs to the technical field of biomedical materials and regenerative medicine, and relates to collagen-based injectable fiber hydrogel as well as a preparation method and application thereof. According to the injectable fiber hydrogel, methacrylated collagen and dithiothreitol are subjected to a thiol-ene click reaction rapidly under the action of a photoinitiator through illumination, a primary cross-linked network is formed, and the injection and in-situ gel forming requirements are met; then under the conditions that the pH is 6.5-8.0 and the temperature is 30-39 DEG C, collagen molecules are self-assembled into a nanofiber structure, and a bionic network similar to a natural extracellular matrix is obtained. The hydrogel disclosed by the invention has the advantages of rapid gelation, structural biomimetic property and biological activity; residual sulfydryl endows the hydrogel with active oxygen scavenging capacity, so that oxidative stress can be relieved, and immune response can be regulated; the fibrotic structure promotes cell adhesion, migration and tissue regeneration.

The invention relates to the technical field of biomedicine, in particular to hyaluronic acid hydrogel for simulating magnetic regulation and control of cell adhesion performance of tumor ECM (extracellular matrix). The hydrogel is prepared from the following components in percentage by weight: 0.5 to 10 percent of thiolated polysaccharide, 0.01 to 2.5 percent of 2-hydroxy-4 '-(2-hydroxyethoxy)-2-methyl propiophenone Irgacure2959, 0 to 100 [mu] g / mL of a superparamagnetic iron oxide nanorod SPIONR, 0 to 100 [mu] g / mL of a cell adhesion ligand and the balance of a solvent. According to the hyaluronic acid hydrogel capable of simulating the magnetic regulation and control of the cell adhesion performance of the tumor ECM, provided by the invention, the isotropic or anisotropic presentation of a cell adhesion ligand is regulated and controlled by magnetic arrangement through the magnetic nanorod coating soft hydrogel with a high aspect ratio; the hydrogel combines RGD modified superparamagnetic nanoparticles with a hyaluronic acid matrix, the arrangement of the nanoparticles is accurately regulated and controlled through an external magnetic field, and dynamic simulation of an ECM anisotropic topological structure is realized.

The invention relates to the field of biological environmental protection, in particular to a heterotrophic nitrification immobilized engineering bacterium for efficient denitrification and a preparation method and application thereof.According to the heterotrophic nitrification immobilized engineering bacterium for efficient denitrification and the preparation method and application of the heterotrophic nitrification immobilized engineering bacterium, polyethylene glycol-glucan two-aqueous-phase concentration and trehalose protection liquid are adopted for synergistic effect, the bottleneck of cell damage caused by a traditional flocculation method can be avoided, trehalose molecules can wrap bacterial cells, and the bacterial cells can be effectively degraded; the immobilized particles can resist osmotic pressure impact and pollutant invasion, the survival rate of thalli is higher than 95%, so that the immobilized particles always keep ultrahigh denitrifying enzyme activity in various sewage environments, honeycomb-shaped pores are formed in an ethyl cellulose matrix, a microenvironment with a high specific surface area is provided for thalli attachment, and the built-in nano zeolite continuously and slowly releases Cu < 2 + > and Zn < 2 + > trace elements, so that the immobilized particles can be recycled. A thallus metabolism enzyme system is synergistically activated, and the denitrification pathway function is enhanced; the chitosan-polydopamine outer coating layer strongly captures the nitrogen-containing pollutants, so that the removal efficiency of the nitrogen-containing pollutants is improved; through triple protection, the density of unit granular bacteria is up to 109 cells / cm, the service life is prolonged, and the denitrification performance is improved by more than 40%.

The invention provides a serum-free stem cell culture medium and a preparation method thereof, and the culture medium realizes long-term stable culture of stem cells under a completely serum-free condition by optimizing basic culture medium components and adding a specific growth factor combination, an extracellular matrix simulant and a metabolism regulator. The culture medium comprises a basic culture medium, a recombinant human protein substitute, a cell adhesion promoting factor, a growth factor combination, an antioxidant and a metabolism regulator. Compared with the prior art, the invention has the following advantages: 1) animal-derived components are completely avoided, and the immunogenicity and the difference between batches are reduced; 2) maintaining the dryness of the stem cells through a specific growth factor combination; 3) adding a metabolism regulator to optimize cell energy metabolism; 4) the cost is obviously lower than that of commercially available like products; and 5) supporting long-term culture of stem cells without spontaneous differentiation. The invention also provides a preparation method of the culture medium and a method for culturing stem cells by using the culture medium.

The invention discloses a corrosion-resistant and collagen-functionalized magnesium-based metal coating and a preparation method of the corrosion-resistant and collagen-functionalized magnesium-based metal coating. Belongs to the technical field of biomedical material preparation. The preparation method comprises the following steps: firstly introducing hydroxyl into the surface of the magnesium alloy through alkali treatment, then sequentially dipping the magnesium alloy into a solution A and a solution B containing specific copolymers, drying to obtain an anti-corrosion coating, and finally coating with a recombinant humanized collagen solution. The copolymer A in the solution A and a magnesium substrate form a firm Si-O-Mg covalent bond through a silane coupling agent, and the fluorine-containing copolymer B in the solution B is tightly combined with a bottom layer through self-assembly, so that a super-hydrophobic anti-corrosion barrier with a stable structure and strong adhesion is constructed. The outermost collagen layer endows the magnesium alloy material with excellent endothelial cell adhesion and proliferation promoting activity. The multi-layer composite coating structure effectively solves the problems that magnesium alloy degradation is too fast, and an existing coating is weak in binding force and lacks biological functions, and has wide application prospects in implantable medical devices such as intravascular stents.

The invention provides an in-situ tissue engineering valve stent material as well as a preparation method and application thereof, and belongs to the technical field of medical materials. According to the invention, the surface of the acellular matrix is loaded with the super-particle coating formed by self-assembly of the polyphenol-mediated polypeptide aggregate and the Prussian blue nano-enzyme, so that the stent material is endowed with double ROS regulation and endothelial cell adhesion promoting functions, excessive deposition of collagenous fibers can be prevented, and fibrosis of valve tissues is avoided; the synergistic effect of the components of the super-particle coating promotes the endothelialization process of the stent and the remodeling of a functional extracellular matrix, so that the accelerated formation of the functional endodermis is finally realized, and a powerful guarantee is provided for the success of clinical transformation of an in-situ heart valve tissue engineering technology.

The invention relates to the technical field of biomedical materials, and discloses an active composite membrane with an asymmetric double-layer structure as well as a preparation method and application of the active composite membrane. The active composite membrane with the asymmetric double-layer structure is prepared from the following preparation raw materials in percentage by mass: 95 to 98 percent of chitosan; 0.5%-2% of a plant polyphenol cross-linking agent; and 1%-4% of magnesium-based degradable inorganic filler. The active composite membrane has an integrated double-layer structure without interface bonding, the compact layer can effectively block soft tissues, and the porous layer is beneficial to cell adhesion and nutrition delivery; in a wet state, the material is excellent in mechanical property, controllable in degradation and high in cell safety, can effectively inhibit bacteria and promote osteoblast proliferation and differentiation, and can meet the performance requirements of bone defect repair on the material; the preparation method adopts the steps of solvent volatilization, freeze drying and precooled alkaline alcohol solution washing for preparation, the whole preparation process is carried out in a water phase, the conditions are mild, the steps are simple, complex equipment is not needed, and extremely high clinical transformation and industrialization potential is shown.

The invention discloses an organic-inorganic multi-layer composite coating material with functions of promoting cell adhesion, resisting infection and promoting osteogenesis and a preparation method of the organic-inorganic multi-layer composite coating material. The method specifically comprises the following steps: 1) carrying out primary sand blasting treatment on a titanium / titanium alloy sample, then removing an oxide layer on the surface of the titanium / titanium alloy sample by using an ultrasonic oil removal and glow discharge cleaning process, and constructing a copper / zinc alloy coating on the surface of the sample by combining an electrochemical deposition process; 2) carrying out micro-nano processing on the coating by using a secondary sand blasting process to introduce a topological morphology; (3) depositing a polydopamine PDA coating on the surface by using an in-situ auto-polymerization process, and simultaneously dipping to complete the chelation of Fe < 3 + > and PDA; 4) further utilizing an ultrasonic impregnation process to realize conversion from PDA-Fe < 3 + > chelation to PDA-Fe < 3 + >-salvianolic acid b chelation so as to introduce a salvianolic acid b layer, and washing, air-drying and sterilizing to obtain the material. The material has multiple cell adhesion promotion, infection resistance and multiple osteogenesis promotion functions, and can realize innovation of coating technologies in the fields of orthopedics, dentistry and the like.

The invention relates to biomedical engineering, and discloses a biochip screening method of a therapeutic agent based on intestinal brain axis regulation and application thereof. The screening method comprises the following steps: performing intestinal wall cell culture or microglial cell culture by adopting a metasurface plasmon resonance biochip of which the surface is subjected to bionic treatment to obtain an intestinal wall cell adherent chip board or a microglial cell adherent chip board; the method comprises the following steps: adding a culture medium containing a chemotherapeutic drug or lipopolysaccharide into a cell-adherent chip board for culture I, then removing the culture medium, adding a therapeutic agent to be detected for culture II to obtain a culture solution, and detecting the cell repair effect of intestinal wall cells or microglial cells in the culture solution, and screening to obtain a therapeutic agent with a repairing effect on intestinal wall cells and / or microglial cells. The screening method can be used for efficiently and accurately screening the therapeutic agent with dual effects of intestinal regulation and neuroprotection from a plurality of therapeutic agents to be detected, and can be used for dynamically monitoring and exploring the interaction condition of the therapeutic agent and cells on line in an unmarked and real-time manner.

The invention relates to a polylactic acid microcarrier and a method for cell amplification by using the polylactic acid microcarrier. The preparation method disclosed by the invention is simple and convenient to operate, the prepared polylactic acid microcarrier is uniform in particle size and pore size distribution, the microcarrier is subjected to surface retreatment, more active sites are provided for cell adhesion, a three-dimensional matrix environment closer to a body is provided, cell amplification is facilitated, the dryness and differentiation potential of stem cells are maintained, and the preparation method is suitable for large-scale popularization and application. And the microcarrier can be repeatedly used, so that the use cost is reduced.

The invention relates to the technical field of preparation of polycaprolactone porous membranes, in particular to a polycaprolactone porous membrane and a preparation method and application thereof.The polycaprolactone porous membrane is of a porous structure with macropores and small pores coexisting, the pore walls of the macropores and the small pores are all composed of polycaprolactone particles, and the polycaprolactone porous membrane is provided with pores with the pore walls composed of micron-sized particles. The polycaprolactone porous membrane is prepared from polycaprolactone, a composite hierarchical pore structure containing small pores in macropores is formed, a three-dimensional pore network internally communicated with one another is formed, the specific surface area is increased by the porous structure, the number of adhered cells is further increased, the interconnected porous structure allows cells to migrate into the polycaprolactone porous membrane, and meanwhile, the porous structure is convenient for conveying substances.

The present invention relates to a polymer conjugate comprising a ULA polymer a) and one or more polypeptides b), wherein the polypeptides are conjugated to the ULA polymer, and the conjugate is coated on the surface of a device for culturing cells. The peptides can interact with receptors on the surface of the cells, while the polymer prevents non-specific binding of the cells to the surface of the device.

In some aspects, the present disclosure provides systems for forming hydrogels that comprise a cell-adhesive polyamino compound and a reactive multi-arm polymer that comprises a plurality of hydrophilic polymer arms having reactive end groups that are reactive with amino groups of the cell-adhesive polyamino compound. In other aspects, the present disclosure provides crosslinked reaction products of a cell-adhesive polyamino compound and a reactive multi-arm polymer that comprises a plurality of hydrophilic polymer arms having reactive end groups that are reactive with amino groups of the cell-adhesive polyamino compound. In other aspects, the present disclosure pertains to methods of treatment using such systems and crosslinked products.

The invention relates to application of golden larch bark acetic acid in preparation of methicillin-resistant staphylococcus aureus (MRSA) biological membrane and bacterium holding inhibitor. A biological membrane formation inhibition experiment shows that the pseudolaric acid can remarkably inhibit formation of an MRSA biological membrane, a fluorescent quantitative PCR experiment shows that the pseudolaric acid remarkably reduces transcription of related genes of the MRSA biological membrane, and a persistent bacterium killing experiment shows that the pseudolaric acid enhances the killing capability of gentamicin on MRSA persistent bacteria. Lung epithelial cell adhesion tests find that the golden larch bark acetic acid significantly reduces the adhesion effect of the MRSA strain on epithelial cells. A test for constructing an MRSA infected greater wax moth model shows that the golden larch bark acetic acid has a system protection effect on the MRSA infected greater wax moth model.

Methods of providing biologically synthesized human structural material Type VI collagen are provided. The collagen of the present application comprises an amino acid sequence set forth in SEQ ID NO: 2 or a variant amino acid sequence that is mutated from the amino acid sequence set forth in SEQ ID NO: 2, the variant amino acid sequence retaining the function of the amino acid sequence set forth in SEQ ID NO: 2. The collagen of the present application is capable of promoting cell adhesion and has a triple helix structure.

The invention relates to a preparation method of a plastic bone grafting material and the plastic bone grafting material.The preparation method comprises the following steps that a, phenyl phosphate lithium salt is dissolved in a phosphoric acid buffer salt solution to obtain a mixed solution, and Ge MA is dissolved in the mixed solution to obtain a Ge MA gel solution; b, bone meal and the Ge MA gel solution are subjected to a cross-linking effect, so that the bone meal and the Ge MA gel solution are fully mixed, then light source irradiation is conducted, the bone meal hydrogel bone grafting material is obtained, and the particle size of the bone meal is 0.25-0.5 mm. According to the preparation method of the plastic bone grafting material, provided by the invention, the bone meal and the Ge MA gel solution are subjected to cross-linking and then subjected to light source irradiation, so that the bone meal and the hydrogel are uniformly mixed, the compatibility of an organic phase and a bone meal particle interface is good, and the prepared plastic bone grafting material is relatively high in plasticity and good in osteoinductivity; and the cell adhesion rate is high.

This invention discloses an interlocking bone tissue repair scaffold, assembled from several notched links connected in an interlocking manner. The notches in one link allow passage through the notches in another link, but not through the remaining main body of the other link. The links are 3D printed from calcium phosphate ceramic powder. The porosity of the final assembled chain can be flexibly controlled by adjusting the shape, size, thickness, and combination of links of different sizes, allowing for precise customization according to specific application scenarios. This scaffold structure is flexible and adjustable, adaptable to different implantation areas, significantly expanding the product's applicability. After implantation, it creates a microenvironment more closely aligned with human tissue growth patterns, optimizing local tissue compatibility and providing excellent conditions for cell adhesion, retention, and proliferation, effectively improving the scaffold's biocompatibility and clinical application efficacy.

The invention provides a method for promoting differentiation of induced pluripotent stem cells to trigerm layers and application of the method in differentiation potential detection, and relates to the technical field of biologication.The method comprises the steps that the induced pluripotent stem cells are inoculated into a culture medium containing 5-20 [mu] M of E-cadherin activator 1, shaking culture is conducted to form embryoid bodies, the embryoid bodies are added into a differentiation culture medium for differentiation culture, and the embryoid bodies are obtained. Obtaining an embryoid body for activating three-germ-layer differentiation potential; inoculating the embryoid body which activates the differentiation potential of the three-germ layer into a container coated with a cell adhesion matrix, adding a differentiation culture medium, continuing differentiation culture, and performing induced differentiation to form the three-germ layer cells. The technical problems that in the prior art, when iPSC is induced to be differentiated to three germ layers through an embryoid body path, the embryoid body forming capacity is poor, the embryoid body state is poor, the long-term maintaining capacity is poor, and the endoderm differentiation and climbing-out capacity is poor due to the fact that the embryoid body is poor in wall attaching capacity after plate rotation are solved.

The application relates to the technical field of image processing, and discloses a urine formed element instance segmentation method, which comprises the following steps: constructing a urine formed element labeled image dataset, dividing the urine formed element labeled image dataset into a training set and a test set; taking a Mask Scoring R-CNN model as a basic model, training an instance segmentation model integrated with an SE attention mechanism by using the training set; inputting urine formed element labeled data in the test set into the trained instance segmentation model to perform instance segmentation, and obtaining a urine formed element segmentation result. On the basis of the Mask Scoring R-CNN model, the SE attention mechanism is integrated, the urine formed element image is subjected to instance segmentation, the problems of urine formed element missed detection and rough urine formed element segmentation caused by cell adhesion, overlapping and impurities can be effectively solved, and the precision and efficiency of urine formed element instance segmentation are improved.

The utility model discloses a differential adherent cell separation device which comprises a separation box body and an upper box cover, culture cavities and a one-way conducting assembly are arranged in the separation box body, the one-way conducting assembly is arranged between the culture cavities, the side face of the separation box body extends outwards to form a supporting part, the side edge of the supporting part protrudes upwards to form a baffle ring part, and the baffle ring part is connected with the upper box cover. A limiting slot is formed among the baffle ring part, the supporting part and the separating box body, and the four sides of the upper box cover extend downwards to form side ring parts. According to the utility model, the one-way conduction between the culture cavities is realized through the one-way conduction assembly, the communication state between the culture cavities can be controlled at a proper stage according to the actual condition of cell wall attachment, the cells are ensured to flow according to the expected direction and mode, and the accuracy and purity of cell separation are effectively improved; the upper box cover and the separation box body are tightly and stably connected, the upper box cover is prevented from loosening or displacing in the using process, and the overall sealing performance and stability of the device are guaranteed.

A biomimetic intervertebral fusion device with a piezoelectric multifunctional coating belongs to the field of implantable medical devices. It includes an upper piezoelectric multifunctional coating, a fusion device body, and a lower piezoelectric multifunctional coating. A bone graft window is located in the middle of the biomimetic intervertebral fusion device. The piezoelectric multifunctional coating is made of electrospun piezoelectric PVDF / HA fibers, which are conformally adhered to the upper and lower surfaces of the fusion device body through a polydopamine adhesive layer. The upper and lower surfaces of the fusion device body have a biomimetic curved surface configuration to match the morphology of the vertebral endplates, increasing the contact area and reducing stress concentration at the fusion interface. While maintaining an elastic modulus similar to natural bone and good radiation permeability, this fusion device, after implantation, can create a microenvironment conducive to cell adhesion and proliferation. Under the influence of cell traction, ultrasonic stimulation, and the physiological load of the human lumbar spine, it generates electrical stimulation signals, promoting bone fusion, improving the stability of the fusion interface, and reducing the risk of postoperative loosening and subsidence of the fusion device.

The invention discloses a hydrogel composition for three-dimensional culture of tumor cells and a preparation method of the hydrogel composition. The hydrogel composition is prepared from poly (lactic acid-glycolic acid) copolymer-polyethylene glycol-poly (lactic acid-glycolic acid) copolymer PLGA-PEG-PLGA and methylacryloyl gelatin GelMA, the mass ratio of the poly (lactic acid-glycolic acid) copolymer-polyethylene glycol-poly (lactic acid-glycolic acid) copolymer PLGA-PEG-PLGA to the methylacryloyl gelatin GelMA is (2-5): 10. According to the hydrogel composition, methacrylated gelatin GelMA is used as a main substrate, so that good biocompatibility and cell adhesion are provided; according to the invention, polylactic acid-glycolic acid copolymer-polyethylene glycol-polylactic acid-glycolic acid copolymer PLGA-PEG-PLGA is used as an auxiliary component, lactic acid is generated through biodegradation of the polylactic acid-glycolic acid copolymer-polyethylene glycol-polylactic acid-glycolic acid copolymer-PEG-PLGA, the pH of a microenvironment is dynamically reduced to 6.5-6.9, tumor acidic conditions are simulated, and pores formed after degradation can provide more growth and invasion spaces for tumor cells.

The invention relates to the technical field of medical biological materials, and discloses a mussel mucin-chondroitin sulfate composite hydrogel and a preparation method and application thereof, and the preparation method comprises the following steps: respectively preparing a mussel mucin aqueous solution and a chondroitin sulfate aqueous solution; the mass volume concentration of the mussel mucoprotein aqueous solution is 1%-10%, the mass volume concentration of the chondroitin sulfate aqueous solution is 1%-10%, the mass ratio of the mussel mucoprotein to the chondroitin sulfate is 3: 1-1: 3, the two solutions are mixed and stirred or oscillated under the conditions that the temperature is 4-37 DEG C and the pH value is 7.0-9.0, a cross-linking reaction is conducted, and hydrogel is formed; the cross-linking mode is physical cross-linking, chemical cross-linking or physical-chemical dual cross-linking; according to the invention, excellent tissue adhesion, injectability, self-healing property and biodegradability are realized, cell adhesion and proliferation can be effectively promoted, and the gel is suitable for various medical beauty scenes such as skin filling, wound repairing and anti-aging nursing.

The invention relates to the technical field of cell biology, and particularly discloses an isolated culture method of primary meibomian gland epithelial cells, the obtained primary meibomian gland epithelial cells and application of the primary meibomian gland epithelial cells. The method comprises the following steps: efficiently and mildly digesting meibomian gland tissues by adopting a compound digestive juice consisting of I-type collagenase, dispersing enzyme and trypsin in a specific ratio; stopping digestion by using a serum-containing neutralizing solution and collecting cells; the method comprises the following steps: promoting cell attachment by using a serum-containing initial culture medium, and carrying out selective amplification by using a serum-free proliferation culture medium to finally obtain high-purity and high-activity primary meibomian gland epithelial cells. The method solves the problems of long digestion time, low cell yield, poor purity and the like in the traditional method. The obtained cell can be used for constructing a meibomian gland dysfunction model, screening drugs and researching lipid metabolism, and an important technical platform and a cell model are provided for research and treatment of related eye diseases.

The present invention relates to a method for the preparation of an assembly of cells and microrobots comprising (a) preparing a suspension of cells and microrobots, wherein the microrobots comprises an alginate, collagen, fibrin, hyaluronic acid / collagen, polyacrylamide, or polyethylene glycol hydrogel capsule, wherein the alginate is conjugated to cell adhesive peptides, and (b) incubating the suspension of (a) to allow the microrobots to form an assembly with the cells via the binding of the cell adhesive peptides to the cells, wherein step (b) and preferably steps (a) and (b) are performed in a conic microcentrifuge tube with a rounded bottom without corners and edges.