548 results about "Cell adhesion" patented technology

The invention provides humanized recombinant XVII collagen for promoting hair follicle stem cell repair and resisting aging as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The humanized recombinant XVII type collagen comprises polypeptide derived from the XVII type collagen or repeated protein formed by N times of repeated series connection of the polypeptide serving as a unit, n is an integer from 2 to 4; the polypeptide derived from the XVII type collagen comprises a polypeptide A and / or a polypeptide B; the amino acid sequence of the A polypeptide is as shown in SEQ ID NO: 1; and the amino acid sequence of the B polypeptide is as shown in SEQ ID NO: 2. The humanized recombinant XVII type collagen not only can be massively and stably recombined and expressed, but also has cell adhesion activity, cell proliferation promotion activity, cell migration promotion activity and excellent hair follicle repair and regeneration promotion activity, and has no sensitization side effect. The invention provides a new way for preparing collagen products with rich functions.

The invention relates to a recombinant A type humanized XVII type collagen as well as construction and application thereof, the protein is the humanized XVII type collagen which is composed of an intracellular region, a transmembrane region and an extracellular region, and the amino acid sequence is as shown in SEQ No. 1. The hair follicle repairing agent has the advantages of hair follicle repairing, hair regeneration, good biological activities of cell proliferation, cell adhesion and the like, and can be widely applied to the fields of medicines, medical instruments, biological materials, tissue engineering, cosmetics and the like. The gene sequence of the recombinant A type XVII collagen is 100% identical to the corresponding part of the gene sequence of the recombinant A type XVII collagen, so that the recombinant A type XVII collagen has good hydrophilicity and high activity, and has application potential in the fields of biomedical materials, beauty cosmetics, skin care products and the like.

The invention discloses humanized III-type collagen as well as a preparation method and application thereof, and belongs to the technical field of bioengineering. According to the method, firstly, on the basis of an original humanized III-type collagen gene, cell adhesion activity is taken as an evaluation index, a better cell adhesion sequence is screened out and spliced and integrated with the original collagen gene, and brand-new humanized III-type collagen is obtained. And constructing a recombinant expression vector of the humanized III type collagen containing the adhesion sequence by taking the probiotic EcN as a host cell, and introducing the recombinant expression vector into the EcN to obtain a strain of functional recombinant probiotic. Compared with a traditional escherichia coli engineering bacterium BL21 (DE3), the recombinant probiotics have good cell adhesion activity while efficiently expressing the humanized type III collagen, the bacterial endotoxin content of the recombinant humanized type III collagen obtained through purification meets the industrial standard, and the recombinant probiotics have good application prospects. The purification cost caused by industrial removal of endotoxin can be avoided, so that the production cost is reduced, and the method has a good application prospect.

The invention relates to a protein-based phenylboronic acid ester dynamic crosslinking hydrogel material as well as a preparation method and application thereof. The preparation method comprises the following steps: dissolving a component A, namely a phenylboronic acid modified protein polymer derivative, in a biocompatible medium to obtain a solution A; dissolving a component B, namely a macromolecular derivative containing a vicinal diol group, into a biocompatible medium to obtain a solution B; and mixing the solution A and the solution B with a certain concentration for a certain time to obtain the protein-based phenylboronic acid ester dynamic crosslinking hydrogel material. The boric acid ester dynamic cross-linking bond belongs to reversible chemical cross-linking reaction, and the prepared protein-based hydrogel material has enough mechanical strength and cheap operability. The protein-based hydrogel material disclosed by the invention can provide the effects of cell adhesion, proliferation and differentiation promotion, tissue regeneration and the like, can load and slowly release beneficial components for promoting tissue repair, is an ideal tissue engineering scaffold material, and can be applied to defect regeneration and repair of soft tissues, cartilages, bones and the like.

The present invention relates to a recombinant human type IV collagen and a preparation method therefor, which belong to the technical field of bioengineering. A recombinant human type IV collagen is designed on the basis of a partial sequence in an α2 chain of human type-IV collagen, and DNA encoding of the protein is ligated into an expression vector to construct a recombinant expression vector; then the recombinant expression vector is transformed into competent Escherichia coli cells, and a recombinant expression plasmid is extracted; after the plasmid is linearized, the linearized plasmid is transformed into an engineered strain HCPB-PPKEX2 to obtain a recombinant engineered strain; and the engineered strain is cultured, the expression of a target protein is induced, and the target protein is purified and identified. It is further demonstrated experimentally that the collagen can be completely cleaved by Kex2, Ste13 and CPB enzymes and efficiently secreted and expressed extracellularly, and the obtained sequence is completely consistent with a theoretical sequence. It is also verified experimentally that the recombinant collagen has a cell adhesion activity and a cell migration-promoting activity. The method avoids the risk of residual exogenous proteins, shortens the time and reduces the cost of the subsequent purification process, and thus has a relatively high safety and application value.

The invention relates to the technical field of bioengineering, in particular to a recombinant VII type collagen for inhibiting scars as well as a preparation method and application of the recombinant VII type collagen. The amino acid sequence of the recombinant VII type collagen is as shown in SEQ ID NO. 1. The recombinant VII type collagen provided by the invention has a better cell adhesion function, so that the recombinant VII type collagen can be applied to the fields of preparation of tissue materials, wound dressings and the like. Meanwhile, the VII type collagen does not contain any tag or exogenous amino acid sequence, is a completely humanized sequence, and does not generate immune response. The VII type collagen has a good adhesion effect, the adhesion effect is better than that of other recombinant collagen, the adhesion effect is equivalent to that of a common cell adhesive in the market, scars can be reduced by inhibiting activation of a TGF-beta pathway, and the VII type collagen can be developed into skin care products and medical instruments for promoting traceless wound repair and has a wide application prospect.

The invention relates to medical recombinant collagen repair gel and a preparation method thereof, and belongs to the technical field of recombinant collagen. Through the synergistic effect of the collagen, the chitosan, the polylactic acid, the nanocellulose, the vascular endothelial growth factor, the anti-inflammatory drug and the cell adhesion peptide, the tissue repair effect is remarkably improved. Through a gas foaming method, staged freezing and freeze drying, multistage pore size distribution is generated, gel with a porous structure is formed, and cell permeation and tissue regeneration are facilitated; in addition, the permeability and the drug loading capacity of the gel are improved, the cell adhesion peptides, the growth factors and the anti-inflammatory drugs are better adsorbed to the gel, functional slow release is achieved, and the repairing effect is further improved.

The invention discloses a recombinant human IV-type collagen as well as a preparation method and application thereof, and belongs to the field of bioengineering and protein engineering. According to the invention, three recombinant human IV type collagen new molecules are designed and screened on the basis of a part of sequences in a human IV type collagen alpha1 chain, soluble expression of a target protein is realized by using an escherichia coli system, and the recombinant collagen with the purity of more than 98% can be obtained through nickel column purification and acid regulation treatment. The recombinant collagen provided by the invention has good cell migration promotion activity and cell adhesion activity, and can be widely applied to the fields of tissue engineering, medical equipment, cosmetics, health care products and the like.

The invention provides recombinant IV type collagen and application thereof, and relates to the technical field of protein engineering. In the invention, an amino acid residue repetitive unit with good biological activity and stability in the human-derived IV-type collagen is found, and the amino acid residue repetitive unit is used as a core for repeating and constructing the recombinant IV-type collagen, so that the novel human-derived recombinant IV-type collagen with biological activity is obtained. The human-derived recombinant IV-type collagen provided by the invention is a small-molecular-weight protein, is easy to absorb by a human body, is good in safety, can remarkably promote secretion of collagen, also has the effects of promoting cell adhesion and cell migration, and has a good application prospect in the aspects of basement membrane repair and remodeling. A new functional component is provided for products for repairing and tightening the skin, resisting aging, resisting wrinkles, preserving moisture and the like.

The invention provides a methacrylic anhydride modified sclera extracellular matrix repair material and a curing method thereof, and belongs to the technical field of medical repair materials. The methacrylic anhydride modified sclera extracellular matrix repair material provided by the invention is prepared from the following raw materials: a methacrylic anhydride modified sclera extracellular matrix, methacrylated gelatin, a photoinitiator and a solvent. The sclera extracellular matrix modified by methacrylic anhydride is adopted, methacrylic anhydride contains methacrylic acid, double bonds can be introduced, the sclera extracellular matrix has the photo-initiation polymerization capacity, and in-situ gelling can be carried out at sclera and cornea damage positions under the action of a photoinitiator; the sclera extracellular matrix has good biocompatibility and histocompatibility, so that sclera matrix cells and corneal epithelial cells are adhered and proliferated, and the sutureless repair is realized; the methacrylated gelatin can provide a photo-crosslinking gelatin network under the action of a photoinitiator, so that cell adhesion and proliferation can be promoted, and repair is realized.

The invention discloses recombinant IV-type collagen as well as a preparation method and application thereof, and belongs to the technical field of bioengineering. The amino acid sequence of the recombinant IV type collagen comprises a plurality of repetitive units, and the repetitive units are shown as SEQ ID NO. 1. The recombinant IV type collagen fragment is derived from human IV type collagen, heterologous amino acid residues are not introduced, and rejection and sensitization risks are avoided; meanwhile, the hydrogel also has good biological activity, including cell proliferation promoting activity and cell adhesion promoting activity, and is good in biocompatibility. In addition, the preparation method of the recombinant IV-type collagen is simple and easy to operate, and the recombinant IV-type collagen with high purity and stability can be obtained through the fermentation and purification method. Therefore, the recombinant IV type collagen disclosed by the invention has a relatively good application prospect.

The invention discloses a preparation method of antibacterial and hemostatic medical sponge, and relates to the technical field of preparation of medical sponge. The preparation method of the antibacterial hemostatic medical sponge comprises the following steps: cleaning silkworm cocoons after removing impurities, and immersing the cleaned silkworm cocoons in a neutral protease solution to complete degumming so as to obtain fibroin fibers; the preparation method comprises the following steps: dissolving chitosan in an acetic acid solution, dissolving polyvinyl alcohol in deionized water, adding sodium alginate, adding a silk fibroin solution to form a composite solution, dropwise adding an AgNO3 solution, introducing CO2, maintaining the pressure, quickly releasing the pressure, foaming, injecting into a mold, carrying out gradient freezing to form a multistage-aperture sponge matrix, immersing in a genipin ethanol solution, and transferring into a 5% CaC 12 solution for soaking. The silk fibroin provides a biocompatible substrate and promotes cell adhesion, the sponge is constructed into a hierarchical pore structure with a compact surface and a loose interior, and liquid absorption and blood coagulation factor enrichment are accelerated.

The invention provides transdermal recombinant keratin as well as a preparation method and application thereof, and relates to the technical field of genetic engineering. The transdermal recombinant keratin comprises a recombinant keratin and a cell penetrating peptide; the transdermal recombinant keratin has cell adhesion activity and transdermal property. The transdermal recombinant keratin comprises any one amino acid sequence as shown in SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3 and SEQ ID NO.4 or consists of the amino acid sequence as shown in SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3 and SEQ ID NO.4. According to the transdermal recombinant keratin, the transdermal performance and the cell adhesion activity of keratin are remarkably improved, and the problem that traditional recombinant keratin is poor in skin permeability is solved.

The present invention belongs to the technical field of optimized coding gene, and discloses a recombinant human type I collagen, a preparation method thereof and an application thereof, and its amino acid sequence is shown as SEQ ID No.1. The recombinant type I collagen provided by the present invention has a high expression level, has better cell migration promotion, cell proliferation promotion and cell adhesion promotion properties, has high safety, and can be widely applied to fields such as medicine, medical devices, biological materials, tissue engineering, cosmetics and the like.

The invention discloses starch-collagen composite hydrogel as well as a preparation method and application thereof. Collagen is catalyzed by microbial transglutaminase to form a covalent cross-linked network, and meanwhile, hydroxypropyl starch is introduced to construct a physical interpenetrating network through molecular chain penetration and hydrogen-bond interaction; and chondroitin sulfate is further integrated to strengthen the network structure and biological activity through electrostatic interaction. The method is mild in condition and does not need a toxic chemical cross-linking agent, the obtained composite hydrogel has an interpenetrating double-network structure and has excellent mechanical properties, enzymatic degradation resistance and cell affinity, the compression modulus of the composite hydrogel is remarkably improved, the composite hydrogel can keep structural stability for a long time in a collagenase environment, cell adhesion and proliferation can be effectively promoted, and the composite hydrogel has a good application prospect. The hydrogel can be widely applied to tissue engineering scaffolds, wound dressings, drug sustained-release carriers and cartilage repair materials.

Disclosed are drug conjugates comprising a targeting moiety conjugated to a drug molecule, wherein the targeting moiety binds to a cell adhesion moiety and / or receptor expressed on a Schwann cell to mediate targeted delivery of the drug conjugate to the Schwann cell, and wherein the drug molecule modifies expression or activity of a disease-associated molecule, and / or confers a cytotoxic effect, in the Schwann cell. Further disclosed herein are methods of targeted delivery of a drug to the Schwann cells.

Provided is a method for biosynthesis of a human structural material type XVII collagen. The collagen comprises the amino acid sequence shown in SEQ ID NO: 2 or a variant amino acid sequence after mutation of the amino acid sequence. The variant amino acid sequence retains the function of the amino acid sequence as shown in SEQ ID NO: 2. The collagen can promote cell adhesion and has a triple helix structure.

The invention provides a recombinant XVII type collagen fragment, and the protein fragment sequence of the recombinant XVII type collagen fragment is shown as SEQ ID No.1. The recombinant XVII type collagen fragment is screened and designed based on a natural collagen sequence, has a triple helix structure, has good biological activity, can effectively exert functions, and has a good promotion effect on cell migration, cell proliferation and cell adhesion. The method for preparing the recombinant XVII type collagen fragment has the advantages that the process steps are simplified, the expression efficiency of the recombinant protein is improved, and the production cost is reduced. The recombinant XVII type collagen fragment provided by the invention has good mechanical properties and biological activity, and can be used for preparing therapeutic drugs and biological materials.

The invention relates to the technical field of biological materials, in particular to a pH response collagen microneedle patch loaded with antibacterial nanoparticles as well as a preparation method and application of the pH response collagen microneedle patch loaded with the antibacterial nanoparticles, and the microneedle patch is prepared by loading the antibacterial nanoparticles, aldehyde modified F127 and recombinant three-type collagen COL3 on a polymer matrix. When the microneedle patch is applied to a chronic wound of a diabetic patient, the microneedle penetrates through the skin in a minimally invasive mode and directly reaches a wound focus area. Under the irradiation of 808nm near-infrared light, the mesoporous polydopamine absorbs light energy, so that the loaded lyase LysAB2 is quickly released, bacterial cell walls are specifically recognized and cracked, and bacteria are efficiently killed; meanwhile, the collagen hydrogel constructs an appropriate microenvironment at the wound, cell adhesion, proliferation and migration are promoted, and the wound healing process is accelerated. Compared with a traditional diabetes mellitus chronic bacterial infection wound treatment means, the microneedle patch provided by the invention provides an efficient and safe new strategy for treating the chronic bacterial infection wound of a diabetes mellitus patient.

The invention discloses an active bone defect repair material and a preparation method thereof. The bone defect repair material is prepared by compounding collagen extracted from an animal source, a calcined bone matrix and bone morphogenetic protein composite microspheres and molding through a photocuring 3D printing technology. From the perspective of bionics, collagen is used as a framework material, a calcined bone matrix with low immunogenicity is used as a hard material to enhance mechanical support of the material, and bone morphogenetic protein composite microspheres have a synergistic effect, so that undifferentiated mesenchymal stem cells are guided to develop towards a specific direction in a long-acting manner. After the active bone defect repair material is implanted into a bone defect part, a microenvironment beneficial to cell adhesion and proliferation is provided for cells, and meanwhile, bone morphogenetic protein is slowly released and can act on the bone defect part for a long time, so that the differentiation and maturation process of osteoblasts is effectively promoted, and bone repair of the bone defect part is promoted.

The invention relates to the technical field of synthetic biology, in particular to polypeptide with a triple helix structure, recombinant XII type humanized collagen and application of the recombinant XII type humanized collagen. The recombinant XII type humanized collagen is successfully expressed and prepared, has a triple-helix structure and good biological activity including promotion of cell proliferation activity, cell adhesion activity and inhibition of MMP-1, is used as a biological material derived from a human body, does not generate immunological rejection and anaphylactic reaction when applied to the human body, and has a good application prospect. The composition can be used for medical or non-medical application of a plurality of tissues and organs of a human body, filling, compatibilizing or repairing, and promotion of skin compactness or wrinkle resistance and other scenes.

The invention belongs to the technical field of biomedical materials and regenerative medicine, and relates to collagen-based injectable fiber hydrogel as well as a preparation method and application thereof. According to the injectable fiber hydrogel, methacrylated collagen and dithiothreitol are subjected to a thiol-ene click reaction rapidly under the action of a photoinitiator through illumination, a primary cross-linked network is formed, and the injection and in-situ gel forming requirements are met; then under the conditions that the pH is 6.5-8.0 and the temperature is 30-39 DEG C, collagen molecules are self-assembled into a nanofiber structure, and a bionic network similar to a natural extracellular matrix is obtained. The hydrogel disclosed by the invention has the advantages of rapid gelation, structural biomimetic property and biological activity; residual sulfydryl endows the hydrogel with active oxygen scavenging capacity, so that oxidative stress can be relieved, and immune response can be regulated; the fibrotic structure promotes cell adhesion, migration and tissue regeneration.

The invention relates to the technical field of biomedicine, and provides an endometrial repair stent and a preparation method thereof.The endometrial repair stent is prepared by taking a flaky nanofiber composite membrane as a substrate, combining the flaky nanofiber composite membrane with modified hyaluronic acid, then conducting dynamic crosslinking with carboxymethyl chitosan and then conducting compounding with poly N-isopropylacrylamide; the mass ratio of the modified hyaluronic acid to the carboxymethyl chitosan to the poly (N-isopropylacrylamide) is (1-9): (1-9): (1-90); the thickness of the nanofiber composite membrane is 0.1-0.4 mm, and the nanofiber composite membrane can be applied to treatment of intrauterine adhesion. According to the endometrial repair stent, the nanofiber composite membrane and the temperature-sensitive hydrogel are combined, a uterine cavity is fully paved by utilizing the fluidity of the hydrogel, and the three-dimensional structure of the nanofiber can be used for orderly inducing a wound surface, so that the adhesion and proliferation of cells are facilitated, scar formation is reduced, the repair effect is ensured, and regeneration is promoted. The two parts are combined, so that the plasticity of the whole structure is enhanced, the supporting performance is improved, the indwelling time in the uterine cavity is long, and the double effects of repairing and isolating are fully achieved.

Provided herein is a method of biosynthesizing human body structural material type IV collagen. Provided herein is a recombinant collagen including the sequence represented by SEQ ID NO: 1, etc. The recombinant collagen of the present application exhibits excellent cell-adhesion activity. The method of the present application utilizes genetic engineering techniques to produce the recombinant collagen, thereby overcoming the drawbacks of the prior art.

To provide a shellac modified object which can be changed from a state where cell adhesiveness to a mammalian cell is high, to a state where the cell adhesiveness is low.SOLUTION: A shellac modified object is expressed by a formula (1). In the formula, Z is a part excluding a carboxy group modified by a functional group Y in the structure of the shellac, X1 to X4 are a dialkylamino group, alkylamino group, hydroxy group, halogen group, H or the like.SELECTED DRAWING: Figure 1

The invention provides a dual-network hyaluronic acid-silk fibroin gel sponge and a preparation method thereof, and belongs to the technical field of gel sponge materials. The preparation method comprises the following steps: mixing sulfhydrylation modified hyaluronic acid and a silk fibroin solution, and sequentially performing freezing and soft freezing treatment to form the hyaluronic acid-silk fibroin gel sponge with a good shape. In the freezing induction process, hyaluronic acid generates chemical crosslinking through disulfide bonds, silk fibroin forms physical crosslinking through freezing concentration, and finally the stable gel-sponge coexistence body with the double-network structure is formed. The gel sponge has double advantages of gel and sponge. Meanwhile, due to freeze concentration treatment, the gel sponge has a macroporous structure, which is beneficial to potentially promoting adhesion, proliferation and migration of cells; the prepared gel sponge does not introduce additional chemical reagents, the biological safety of the gel sponge is guaranteed, and the gel sponge is suitable for the fields of cartilage / skin repair, tissue filling, wound dressing and the like.

The invention relates to the technical field of biomedicine, in particular to hyaluronic acid hydrogel for simulating magnetic regulation and control of cell adhesion performance of tumor ECM (extracellular matrix). The hydrogel is prepared from the following components in percentage by weight: 0.5 to 10 percent of thiolated polysaccharide, 0.01 to 2.5 percent of 2-hydroxy-4 '-(2-hydroxyethoxy)-2-methyl propiophenone Irgacure2959, 0 to 100 [mu] g / mL of a superparamagnetic iron oxide nanorod SPIONR, 0 to 100 [mu] g / mL of a cell adhesion ligand and the balance of a solvent. According to the hyaluronic acid hydrogel capable of simulating the magnetic regulation and control of the cell adhesion performance of the tumor ECM, provided by the invention, the isotropic or anisotropic presentation of a cell adhesion ligand is regulated and controlled by magnetic arrangement through the magnetic nanorod coating soft hydrogel with a high aspect ratio; the hydrogel combines RGD modified superparamagnetic nanoparticles with a hyaluronic acid matrix, the arrangement of the nanoparticles is accurately regulated and controlled through an external magnetic field, and dynamic simulation of an ECM anisotropic topological structure is realized.

The invention specifically discloses recombinant humanized III-type collagen as well as a preparation method and application thereof, and relates to the technical field of collagen sequence optimization. The invention provides a type III collagen, wherein the type III collagen has an amino acid sequence as shown in SEQ ID No.2. The invention also provides a preparation method of the type III collagen. The III-type collagen provided by the invention not only has no obvious cytotoxicity to cells, but also has an extremely good cell proliferation promoting effect, can also improve cell adhesion, and has an obvious promoting effect on proliferation of human immortalized epidermal cell lines, namely wound healing.

The embodiment of the invention discloses a recombinant III-type humanized collagen as well as a preparation method and application thereof. The amino acid sequence of the recombinant III-type humanized collagen is shown as SEQ ID NO.1, SEQ ID NO.2 or SEQ ID NO.3. The recombinant III-type humanized collagen has the advantages that the recombinant III-type humanized collagen can be used for preparing the recombinant III-type humanized collagen; the recombinant III-type humanized collagen provided by the invention has the activity of promoting cell adhesion, the amino acid sequence of the recombinant III-type humanized collagen is selected from the amino acid sequence of natural collagen, and the recombinant III-type humanized collagen does not generate immune response when being applied to a human body. The characteristic that corynebacterium glutamicum does not secrete endotoxin is utilized, the preparation method is simple, the product can meet the requirements of food, cosmetics, medical instruments and drugs without complex separation, and reference is provided for low-cost industrial production.

The invention relates to the field of biological environmental protection, in particular to a heterotrophic nitrification immobilized engineering bacterium for efficient denitrification and a preparation method and application thereof.According to the heterotrophic nitrification immobilized engineering bacterium for efficient denitrification and the preparation method and application of the heterotrophic nitrification immobilized engineering bacterium, polyethylene glycol-glucan two-aqueous-phase concentration and trehalose protection liquid are adopted for synergistic effect, the bottleneck of cell damage caused by a traditional flocculation method can be avoided, trehalose molecules can wrap bacterial cells, and the bacterial cells can be effectively degraded; the immobilized particles can resist osmotic pressure impact and pollutant invasion, the survival rate of thalli is higher than 95%, so that the immobilized particles always keep ultrahigh denitrifying enzyme activity in various sewage environments, honeycomb-shaped pores are formed in an ethyl cellulose matrix, a microenvironment with a high specific surface area is provided for thalli attachment, and the built-in nano zeolite continuously and slowly releases Cu < 2 + > and Zn < 2 + > trace elements, so that the immobilized particles can be recycled. A thallus metabolism enzyme system is synergistically activated, and the denitrification pathway function is enhanced; the chitosan-polydopamine outer coating layer strongly captures the nitrogen-containing pollutants, so that the removal efficiency of the nitrogen-containing pollutants is improved; through triple protection, the density of unit granular bacteria is up to 109 cells / cm, the service life is prolonged, and the denitrification performance is improved by more than 40%.