50 results about "Artificial cornea" patented technology

The invention discloses an aspheric keratoprosthesis lens column, a keratoprosthesis and an application method of the aspheric keratoprosthesis lens column, the whole lens column is cylindrical, the front surface of the lens column is a convex surface, the rear surface of the lens column is a concave surface, the rear surface of the lens column is a surface close to the retina, the front surface of the lens column is a surface far away from the retina, and the front surface and the rear surface of the lens column are both curved surfaces. And the spherical surfaces are rotationally symmetrical aspheric surfaces. The artificial cornea comprises a lens column and a support, and the lens column is installed on the support. The fixed position of the lens column is that the distance between the vertex of the rear surface of the lens column and the retina is 10-120mm, the focal plane is perpendicular to the optical axis, and the inclination angle of the focal plane is 0 degree. According to the lens column, spherical aberration and coma can be effectively corrected, and the imaging quality is improved; light scattering is reduced, the visual contrast at night and in a low-light environment is improved, and the visual experience of a patient is improved; the deviation tolerance of the implantation position is higher, and the operation difficulty and the risk of postoperative complications are reduced.

The invention provides an ultralow-swelling artificial cornea imitating natural cornea fiber arrangement and a preparation method, and belongs to the technical field of biomedical materials.The preparation method comprises the steps that I-type collagen is dissolved in a PBS buffer solution, a photofunctionalization modified active reagent is added for chemical modification, stirring is conducted in a dark place, freeze drying is conducted, and photofunctionalization collagen is obtained; dissolving the photo-functionalized collagen, and then preparing a collagen fiber membrane through electrostatic spinning; and performing phase change DLP 3D biological printing on the biological ink and the collagenous fiber membrane to obtain the keratoprosthesis with the collagenous fiber sandwich structure. Multi-layer arrangement is achieved through DLP 3D biological printing, the fiber trend of the natural cornea is simulated, and the mechanical anisotropy is remarkably improved; through a composite phase change DLP 3D printing mode of GelMA / HA-DN and photo-crosslinking collagenous fibers, the composite phase change DLP 3D printing material has good manufacturability, maintains high light transmittance and maintains high mechanical strength.

The present invention discloses a one-time implant artificial cornea, belonging to the technical field of in-vivo prosthesis implantation. It solves at least the problems existing in the prior art, such as the need for donor corneas, multiple surgeries, large trauma, and inapplicability to patients with severe corneal blindness. The one-time implant artificial cornea includes a stent and a lens column. The lens column includes a lens column body and a rear disc coaxially arranged at the rear end face of the lens column body. The lens column is detachably connected to the stent coaxially. In the use state, the rear end of the lens column body behind the rear disc protrudes into the eye, the rear disc fits the posterior surface of the cornea, and the stent fits the anterior surface of the cornea. The stent is fixed on the corneal surface by using a suture method. At the same time, the stent and the rear disc clamp the cornea from the front and the back. The present invention also provides a method for using the one-time implant artificial cornea. It has the advantages of one-time implantation, no need for donor corneas, small trauma, and applicability to patients with severe corneal blindness, and has broad application prospects.

The present disclosure relates to a support element (10) for an artificial cornea, the support element being disc-shaped and concentric with a central axis (P) and comprising: a central region (101) and a perimetral (102) region adjacent to a perimeter edge of the support element, the central region comprising a convex face (1011), a concave face (1012) opposed to each other and one central through hole (103) concentric with the central axis; wherein the perimetral region is flared, the surface of the support element is coated with silver, and at least the concave face of the support element comprises a peptide or a pharmaceutical salt thereof attached therein. The present disclosure also relates to an artificial cornea comprising the support element described.

This invention discloses an artificial corneal endothelial graft based on natural degradable proteins, its preparation method, and its application, relating to the field of medical implant materials technology. Currently, artificial corneal endothelial grafts for corneal endothelial decompensation are all non-degradable synthetic polymer materials. This invention uses a natural degradable material as the substrate to prepare the graft. A material preparation stock solution is obtained by mixing a silk fibroin solution extracted from a high-salt solution with a certain proportion of glycerol. The mixed solution is then spin-coated onto a mold using a gradient method, and dried to obtain a silk fibroin film. The obtained silk fibroin film is then circumcised and placed in a mold for hot-press steam fumigation. The resulting silk fibroin film can serve as an artificial corneal endothelial graft, providing a donor source for artificial corneas and possessing potential application value in corneal transplantation.

The present invention discloses a collagen-based artificial cornea with a double-layer structure and the ability to rapidly epithelialize, and a preparation method thereof, comprising the following steps: adding collagen to an acetic acid solution, stirring and dissolving, and adding a plasticizer to obtain a high-concentration collagen solution and a low-concentration collagen solution; electrochemically depositing the high-concentration collagen solution to obtain a first collagen film; photocrosslinking the first collagen film by irradiating the first collagen film with strong ultraviolet light; chemically crosslinking the first collagen film to obtain a compacting layer; pouring the low-concentration collagen solution into a sedimentation tank, immersing the compacting layer, and performing a second crosslinking process. The second electrochemical deposition method obtains a second collagen membrane on the compacting layer; the second collagen membrane is subjected to secondary photocrosslinking by weak ultraviolet irradiation to obtain a loose layer, and the loose layer and the compacting layer are combined to form the double-layer structure and the collagen-based artificial cornea capable of rapid epithelialization. The double-layer structure and the collagen-based artificial cornea capable of rapid epithelialization of the present invention have high light transmittance and mechanical properties that meet clinical requirements: the transmittance at 600nm is greater than 90%, the tensile strength is greater than 1Mpa, and the elastic modulus is greater than 10MPa. The low swelling rate can avoid the product from pressuring the autologous corneal tissue, reduce mechanical stimulation to the eye tissue, and improve comfort after implantation.

The application discloses a composite artificial cornea and a preparation method thereof. The composite artificial cornea comprises a mirror column and a biological skirt. An annular gap is arranged on the inner side edge of the biological skirt. The outer side edge of the mirror column is embedded into the inside of the biological skirt along the gap, and the outer side edge of the mirror column is fixed in the gap through casting liquid. The application has the beneficial effects that: by arranging the annular gap on the inner side edge of the biological skirt, then embedding the outer side edge of the mirror column into the inside of the biological skirt along the gap, a interference fit structure is formed, preliminary fixation is formed between the mirror column and the gap, and the outer side edge of the mirror column is fixed in the gap through the casting liquid, so that the casting liquid can permeate and fill the gap, the mirror column and the biological skirt are connected into an integrated whole, secondary fixation is formed, the connection strength of the composite artificial cornea is improved, and the risk of the mirror column falling off is reduced.

The clamping type artificial cornea comprises a lens column body, a curved surface disc used for being attached to the rear surface of a cornea body is coaxially arranged on the outer ring face, close to the rear end, of the lens column body, a guide hole is formed in the curved surface disc, and the circumferential thickness of the curved surface disc is gradually increased from the center to the edge; the lens column body is detachably connected with a curved surface support matched with the curved surface disc, and the curved surface support is fixed in a sewing mode so as to be attached to the front surface of a cornea body. The curved surface disc is used for clamping a curved sample of a cornea body on the curved surface bracket; through cooperation of the curved-surface support and the curved-surface disc and the guide holes in the curved-surface disc, the cornea is clamped in a non-closed mode, the cornea can be clamped more conveniently, abrasion of the cornea body is reduced, the installation stability of the artificial cornea can be improved, the dead weight of the artificial cornea is reduced, and therefore it is guaranteed that the cornea can not be damaged along with the increase of wearing time. The installation position of the artificial cornea is not prone to deviation, and normal use is guaranteed.

The invention relates to the technical field of keratoprosthesis preparation, in particular to a biomimetic structure keratoprosthesis carrying a PRP microsphere slow release system and a preparation method of the biomimetic structure keratoprosthesis. PRP is encapsulated through PLGA microspheres, and stepped release of growth factors is achieved through degradation controllability of PLGA; the PRP-PLGA microspheres still keep a stable slow-release effect within 14 days in vitro, the problem that growth factors are rapidly inactivated due to direct application of traditional PRP is effectively solved, the treatment window period is remarkably prolonged, and continuous biological activity stimulation needed by cornea repair is met; by utilizing the degradation controllability of the PLGA, the stepped release of the growth factor is realized, so that the stable release effect of the PRP can be maintained for a long time after the artificial cornea is transplanted, the treatment window period after the artificial cornea is transplanted is prolonged, and the continuous biological activity stimulation required by cornea repair is met.

The application discloses a composite artificial cornea skirt preparation device and method, which comprises a turnover plate and driving units installed on both sides of the turnover plate, eye ball fixing cups are arranged on the upper end face of the turnover plate in a matrix mode, the upper end of the eye ball fixing cup is open and used for fixing an eye ball, a decellularization liquid tank is arranged below the turnover plate, the driving units are used for driving the turnover plate to overturn by 180 DEG and sink downwards until the cornea of the eye ball is completely immersed below the liquid level of the decellularization liquid tank, and the device further comprises a stroma plate layer cutting knife, a central stroma ring cutter, a stroma bulking device, a stroma plate layer tunnel knife and a peripheral stroma ring cutter. The application has the beneficial effects that the cleaning, material taking, decellularization, bulking and fixing of the corneal stroma are completed at one time, and the whole process is automatically, standardly and normatively produced, so that the decellularized corneal stroma skirt or a biological cornea is obtained.

The present invention provides a device for measuring large-field imaging of an artificial eye. A medium tank is provided at the upper end of the posterior chamber of the artificial eye. A light-transmitting hole is provided on the side wall of the posterior chamber of the artificial eye, and an artificial cornea and an artificial lens are respectively and hermetically installed in the light-transmitting hole. The medium tank is filled with a liquid medium. The input end of the artificial retina detector is located in the liquid medium, and the periphery of the artificial retina detector is fixedly installed at one end of a support rod. The other end of the support rod is installed on a displacement platform, and the displacement platform can drive the artificial retina detector to displace through the support rod. The lower end of the displacement platform is fixedly installed on the upper end of a substrate, and a support and a light source are respectively arranged at the lower end of the substrate. The device for measuring large-field imaging of an artificial eye according to the present invention can achieve the detection of large-field imaging of the artificial eye. The detector of the artificial retina is waterproofed, so it can be placed in the waterproof liquid of the artificial human eye, thereby approaching the actual imaging of the human eye to the greatest extent.

The application discloses an umbrella-shaped mirror column-based artificial cornea, which comprises a support and a mirror column connected with the support, wherein the support comprises a main body structure in a spherical surface structure, a middle part of the main body structure is provided with an opening, the mirror column is arranged in the opening, a micro-porous gap is formed on the circumferential surface of the main body structure, the top end surface of the mirror column is formed in an arc-shaped umbrella structure, and the bottom end surface of the mirror column is formed in a concave spherical surface structure. The application further discloses a preparation process of the artificial cornea. The artificial cornea is provided with the micro-porous gap on the circumferential surface of the main body structure, so that the healing tissue can pass through the micro-porous gap, the combination force between the support and the cornea is increased, and the stability is improved. In addition, the arc-shaped umbrella structure formed on the top end surface of the mirror column can prevent the growing tissue from covering the mirror surface of the mirror column in the later use of the patient, the light can enter the mirror column, the optical function can be normally played, and the occurrence of the anterior membrane complication is reduced.

The invention provides a method for constructing a tissue engineered cornea based on human amniotic epithelial stem cells and application, and belongs to the technical field of biology. The method comprises the following steps: 1) culturing human amniotic epithelial stem cells in a corneal epithelial cell induced differentiation culture medium to obtain human corneal epithelial cell-like cells; culturing the human amniotic epithelial stem cells in a corneal stroma cell induced differentiation culture medium to obtain corneal stroma cells; and 2) respectively inoculating the human corneal epithelial cell-like cells and corneal stroma cells obtained in the step 1) on the surface and inside of an acellular corneal stroma scaffold, and co-culturing for 5 days to construct the tissue engineered cornea. According to the method, the two corneal cells derived from the human amniotic epithelial stem cells are simultaneously inoculated to the human decellularized corneal stroma for the first time, the tissue-engineered cornea is successfully constructed, in addition, the corneal cells obtained through differentiation can be inoculated to various types of biological materials such as hydrogel, and the tissue-engineered cornea is obtained. Wider application possibility is provided for developing various novel artificial cornea products.

The present invention discloses an artificial cornea utilizing Catalase@nHOFs nanoparticles, belonging to the field of in vivo prosthetic implant technology. This method eliminates the need for frequent dosing and provides long-lasting protection against ROS. Furthermore, it eliminates the need to adjust or alter the existing artificial cornea structure. The portion of the artificial cornea that contacts the eye is coated with a Catalase@nHOFs nanocoating. The coating is prepared by encapsulating catalase within a hydrogen-bonded organic framework to form Catalase@nHOFs nanoparticles, which adhere to the surface of the artificial cornea to form the Catalase@nHOFs nanocoating.

To provide an artificial cornea suitable for surgical implantation.SOLUTION: An embodiment of an artificial cornea includes: an optical element that includes a body having an anterior side and a posterior side and an annular flange extending about the body; and a tissue integration skirt coupled to the optical element, the tissue integration skirt coupled to the optical element. The anterior side of the body includes an anterior optical surface and the posterior side of the body includes a posterior optical surface. The tissue integration skirt is configured to promote tissue ingrowth, and the tissue integration skirt is coupled to the optical element such that at least a portion of a periphery of the annular flange defined between the anterior and posterior sides of the optical element is covered by the tissue integration skirt. Also described are methods for implanting an artificial cornea of the present disclosure, the methods including providing the artificial cornea, removing a section of corneal tissue from the patient's cornea to form a tissue bed of existing tissue to which the artificial cornea can be affixed, implanting the artificial cornea such that the posterior side of the artificial cornea is suspended above the interior of the eye, and mechanically affixing the implanted artificial cornea to the existing corneal tissue of the tissue bed.SELECTED DRAWING: Figure 4

The application discloses a kind of methacrylic acid succinic acid double ester starch and its preparation method and application in corneal defect in situ filling agent, belong to the technical field of biomedical materials.The methacrylic acid succinic acid double ester starch is grafted carboxylation treatment to starch, then graft double bond is prepared.The corneal defect in situ filling agent of the application is composed of methacrylic acid succinic acid double ester starch (StacMA), methacrylated gelatin (GelMA), photo initiator and solvent.The natural biological macromolecule based corneal filling agent of starch and gelatin in the application has good biocompatibility, excellent light response adhesion characteristics and excellent mechanical properties, and the in-situ photocrosslinking of hydrogel is realized by ultraviolet irradiation, while excellent mechanical properties and strong adhesion capacity are generated, to realize the stable adhesion of in-situ forming artificial cornea on corneal implant bed, promote the regeneration of damaged corneal natural structure and the reconstruction of visual function.

The present invention discloses a humanoid eye-mimicking artificial cornea for single implantation, belonging to the technical field of in-vivo prosthesis implantation. It solves at least the problem that the existing artificial corneas cannot simulate the appearance of real eyes. The artificial cornea includes a lens column, a stent and a cosmetic disc; wherein, the lens column includes a lens column body and a rear disc coaxially arranged at a position close to the rear end face of the lens column body; the cosmetic disc is coaxially arranged at the front end of the lens column body; the stent is coaxially and movably arranged on the lens column body between the rear disc and the cosmetic disc; in the use state, the stent and the rear disc clamp the cornea one in front and the other behind; the cosmetic disc has a central opening and has an iris texture area and a sclera texture area; an iris texture layer is formed in the iris texture area, and a mimic conjunctival vascular texture layer is formed in the sclera texture area. The present invention also provides a method for using the humanoid eye-mimicking artificial cornea for single implantation.

The utility model discloses a bracket for an artificial cornea, which is used for improving the bonding strength between the bracket and a chemical central area of the artificial cornea and simultaneously improving the biocompatibility between the bracket and a maternal cornea tissue. The support is a medical metal support and comprises a support body, a central mounting hole penetrating through the support body and a connecting ring coaxially clamped into the central mounting hole from top to bottom, and internal threads are formed in the inner wall of the connecting ring. A hydroxyapatite layer is formed on the surface of the stent body; the material of the connecting ring is the same as or similar to that of a lens column of the artificial cornea; the connecting ring is of a hollow stepped shaft structure, an annular clamping groove is formed in the corner of a stepped shaft, and in the assembled state, the stent body is clamped into the annular clamping groove, so that the connecting ring is prevented from shifting or falling off in the operation.

The present invention discloses a manufacturing method of an artificial cornea for one-time implantation, belonging to the technical field of in-vivo prosthesis implantation. It solves at least the problems existing in the prior art, such as the need for donor corneas, multiple surgeries, large trauma and inapplicability to patients with severe eye diseases. The manufacturing method includes: manufacturing a lens column, which sequentially includes a first threaded section, a second threaded section, an optical axis section, a rear disc and a rear convex section from the front end to the rear end; manufacturing a stent, which includes a stent body and a connecting ring; the stent body is made of metal and is formed by stamping; manufacturing a cosmetic disc, the material of the cosmetic disc is the same as that of the lens column and is formed by injection molding, the cosmetic disc has a central connection hole, and the inner wall of the central connection hole has an internal thread that is screwed with the first threaded section; assembling the lens column, the stent and the cosmetic disc, specifically including: snapping the connecting ring into the central mounting hole of the stent body from top to bottom, and making the stent body snap into the annular limiting groove of the connecting ring to complete the assembly of the stent.

The present invention provides a type II collar button-shaped artificial cornea, which includes a front plate with an optical lens cylinder. A skirt is annularly arranged on the outer side surface of the optical lens cylinder. A gasket is also annularly arranged on the outer side surface of the optical lens cylinder. The gasket is located between the front plate and the skirt, and the gasket is attached to the skirt. The type II collar button-shaped artificial cornea of the present invention can better clamp the eyelids, reduce the shedding rate of the artificial cornea, and can be better fixed in the eye.

The application provides a method for constructing a tissue-engineered cornea based on human amniotic epithelial stem cells and application thereof, and belongs to the technical field of biotechnology. The method comprises the following steps: 1) culturing human amniotic epithelial stem cells in a corneal epithelial cell induction and differentiation culture medium to obtain human corneal epithelial cell-like cells; culturing the human amniotic epithelial stem cells in a corneal stromal cell induction and differentiation culture medium to obtain corneal stromal cells; 2) inoculating the human corneal epithelial cell-like cells and the corneal stromal cells obtained in step 1) on the surface and inside of a decellularized corneal stromal scaffold respectively, and co-culturing for 5 days to construct a tissue-engineered cornea. The application successfully constructs a tissue-engineered cornea by inoculating two kinds of corneal cells derived from human amniotic epithelial stem cells on a human decellularized corneal stromal at the same time for the first time. In addition, the obtained corneal cells can be inoculated on various types of biomaterials such as hydrogels, thereby providing a wider application possibility for developing various types of artificial cornea products.

The invention relates to an artificial corneal endothelium sheet as well as a preparation method and application thereof. The artificial corneal endothelium sheet is prepared from the following raw materials: a hydrophilic monomer, a hydrophobic monomer, a cross-linking agent, an ultraviolet absorption monomer, a blue light absorption monomer and an initiator, the hydrophilic monomer comprises a combination of hydroxyethyl methylacrylate and polyethylene glycol methacrylate, and the hydrophobic monomer comprises a combination of methyl methacrylate and isobutyl methacrylate; in the preparation raw materials, the use amount of the hydrophilic monomer is 80-94 wt%, and the use amount of the hydrophobic monomer is 5-19 wt%. According to the artificial corneal endothelium sheet provided by the invention, the proportion and components of the hydrophilic monomer and the hydrophobic monomer are regulated and controlled, so that the obtained artificial corneal endothelium sheet is moderate in water content and has excellent mechanical properties, adhesion performance and swelling stability.

The invention relates to a polyurethane artificial cornea material with a porous skirt edge design and a preparation method thereof, and belongs to the technical field of elastomer production. Dihydric alcohol with a benzene ring structure is adopted as a chain extender for preparing polyurethane, a prepolymer is synthesized through a one-pot method, then chain extension, crosslinking and vulcanization reaction are conducted, and the polyurethane elastomer is obtained. The polyurethane artificial cornea material with the porous skirt edge design can be obtained by taking the central part and foaming around the central part, the porous skirt edge design provides a growth space for cells, so that the cornea is fixed more firmly, and the porous skirt edge and the central light-transmitting area are made of the same raw material, so that the combination of the combined material is tighter; and in the using process, breakage is not prone to occurring. In addition, the obtained polyurethane material is good in mechanical property, excellent in optical property, stable in chemical property and non-toxic to cells, and can be applied to a substitute material of an artificial cornea.

A keratoprosthesis (100), comprising a support (10) and a lens column (30), the lens column (30) comprising a body (31) and a collar (32). A middle part of the support (10) is provided with a mounting hole (10A), a hole wall of which being provided with an internal thread (111), and the outer peripheral wall of the collar (32) being provided with an external thread (321) fitting with the internal thread (111), so as to detachably assemble the support (10) and the collar (32). The material of the body (31) is polymethyl methacrylate, so as to provide a reliable light path structure, and the collar (32) is titanium metal. The body (31) and the collar (32) are integrally formed, such that the collar (32) is sleeved on the body (31) so as to form a lens column (30), and the outer peripheral wall of the body (31) is provided with a limiting slot (31D) for limiting the collar (32). The support (10) is titanium metal, such that the internal thread (111) and the external thread (321) are metal threads of the same material, and the heat shrinkage ratios of structures of the same material undergoing a temperature change are the same, so that a joint has a high assembly reliability and does not separate or fall off unless an external force is applied thereto. The service life of the artificial cornea is increased, surgical risks are reduced, a patient is protected from secondary injury or being blinded again, and use satisfaction is increased.

The present invention discloses an agarose-based artificial cornea and its preparation method, belonging to the field of biomedical materials. The method includes the following steps: grafting a hydrophobic chain onto the agarose backbone through an esterification reaction, adding the obtained hydrophobic agarose polymer into dimethyl sulfoxide to obtain a clear and transparent solution. Pouring the solution into a mold by casting, then soaking the mold in water. After partial solvent exchange, tearing off the film on the surface of the solution, and the remaining solution forms a soft and opaque hydrogel at the bottom of the mold. Placing the opaque hydrogel on a plastic petri dish, drying it to obtain a transparent agarose-based film. Swelling the film sufficiently in simulated tear fluid to obtain the agarose-based artificial cornea. This artificial cornea has appropriate transparency, mechanical properties, and excellent biocompatibility, and has broad application prospects in ophthalmic implant materials.

The present disclosure relates to a method of manufacturing a keratoprosthesis exhibiting excellent physical properties by stabilizing the interface of an adhesive optical unit and a support unit through a keratoprosthesis mold and UV dose adjustment. The present disclosure can stably adhere an interface between the optical unit and the support unit by manufacturing the optical unit for 1-4 hours using low dose UV in the keratoprosthesis mold, thereby manufacturing the keratoprosthesis in which the optical unit and the support unit are integrated. The artificial cornea manufactured by the manufacturing method has a constant spherical shape and is convenient for center positioning, so that the success rate of an operation can be improved during the operation; the central part of the optical unit shows uniform and excellent physical properties, so that the physical safety in a human body can be further improved; moreover, the interface of the optical unit and the support unit shows excellent physical properties which are equivalent to about 100 times of the existing intraocular pressure, so that the separation of the optical unit and the support unit caused by intraocular pressure in the human body can be prevented at an early stage. Furthermore, by means of the manufacturing method, the eye appearance of a patient to which the artificial cornea is transplanted can be improved, and complications and side effects caused by adhesive substances can be reduced.

The present invention discloses an aspheric artificial cornea lens column, an artificial cornea and an application method thereof. The lens column is cylindrical as a whole, the front surface of the lens column is convex, the rear surface of the lens column is concave, the rear surface of the lens column is the surface close to the retina, the front surface of the lens column is the surface away from the retina, the front surface and the rear surface of the lens column are both curved surfaces, and both are rotationally symmetric aspheric surfaces. The artificial cornea includes a lens column and a bracket, and the lens column is mounted on the bracket. The fixed position of the lens column is: the vertex of the rear surface of the lens column is 10 to 120 mm away from the retina, the focal plane is perpendicular to the optical axis, and the focal plane inclination angle is 0°. The lens column of the present invention can effectively correct spherical aberration and coma, improve imaging quality; help reduce light scattering, improve visual contrast at night and in low-light environments, and improve patients' visual experience; have a higher tolerance for implant position deviation, and reduce the difficulty of surgery and the risk of postoperative complications.

The present invention discloses a multifocal artificial cornea, which includes a stent and a lens cylinder cooperatively connected to the stent. The stent includes a main body structure, and an opening is provided in the middle of the main body structure. The lens cylinder is penetrated in the opening. The lens cylinder includes a light guiding structure with multiple focal points to form corresponding multiple diopters when the light is zoomed through the light guiding structure. The lens cylinder is made of PMMA material. The present invention also discloses a preparation process of the multifocal artificial cornea. The multifocal artificial cornea of the present invention can not only enable patients to achieve a near vision field but also achieve a distant vision field through the multifocal light guiding structure. When looking at a distance, the light energy allocated to the far focal point is increased, resulting in better distant vision. When looking at a close distance, due to reflection and light stimulation, the light energy allocated to the near focal point is increased, resulting in better near vision, achieving the vision fields at both far and near ends and having a true intermediate vision.

The invention discloses rotary fixing tweezers for assembling an artificial cornea and a using method of the rotary fixing tweezers, and belongs to the technical field of medical precision instruments. When the lens column of the artificial cornea and the support are assembled, the rotary fixing tweezers are used for clamping the lens column. When the cylinder is rotated, force is transmitted to the tweezers body through the spring, the tweezers body is driven to rotate, meanwhile, the length of the spring changes, and meanwhile the size of the force borne by the spring is visually displayed through scales. The rotary fixing tweezers for assembling the artificial cornea have the functions of clamping the lens column and rotating the lens column.

The invention discloses an artificial corneal endothelial graft based on natural degradable protein and a preparation method and application thereof, and relates to the technical field of medical implant materials. Currently, the artificial corneal endothelium graft for the corneal endothelium decompensation disease is a non-degradable artificially synthesized polymer material, the graft is prepared by taking a natural degradable material as a base material, a silk fibroin solution extracted from a high-salt solution is blended with glycerol in a certain proportion to obtain a material preparation stock solution, and the stock solution is prepared into the artificial corneal endothelium graft for the corneal endothelium decompensation disease. Coating a mold with the uniformly mixed blending solution in a gradient spin coating manner, and drying to obtain a silk fibroin film; the obtained silk fibroin film is subjected to girdling and then placed in a mold for hot-pressing steam fumigation, and the obtained silk fibroin film can serve as an artificial cornea endothelial graft, provides a donor source for an artificial cornea and has potential application value in cornea transplantation.