67 results about "Artificial cornea" patented technology

The invention belongs to the field of tissue repair in biomedical materials, and relates to an artificial cornea with a heterogeneous fiber microstructure and a preparation method and application thereof. The preparation method of the collagen artificial cornea with the heterogeneous fiber microstructure comprises the following steps: dissolving type I collagen, and forming a collagen membrane assembled by electric field triggering dynamics through an electro-deposition reaction; covering a preset template on the collagen membrane assembled by the electric field triggering dynamics, and exposing the collagen membrane under an ultraviolet light source for crosslinking; placing the collagen membrane assembled by the electric field triggering dynamics in a constant temperature and humidity chamber, and inducing continuous thermodynamic assembly of the collagen; the temperature in the constant-temperature and constant-humidity box is 4-45 DEG C, the time is 3-30 hours, water or a water-containing solution is placed in the constant-temperature and constant-humidity box, and the relative humidity is controlled to be 40%-100%. According to the artificial cornea, the fiber diameter and the compact stacking state of the collagen assembly are considered, and the suture performance requirement is met while the good transparency is achieved.

The invention discloses an aspheric keratoprosthesis lens column, a keratoprosthesis and an application method of the aspheric keratoprosthesis lens column, the whole lens column is cylindrical, the front surface of the lens column is a convex surface, the rear surface of the lens column is a concave surface, the rear surface of the lens column is a surface close to the retina, the front surface of the lens column is a surface far away from the retina, and the front surface and the rear surface of the lens column are both curved surfaces. And the spherical surfaces are rotationally symmetrical aspheric surfaces. The artificial cornea comprises a lens column and a support, and the lens column is installed on the support. The fixed position of the lens column is that the distance between the vertex of the rear surface of the lens column and the retina is 10-120mm, the focal plane is perpendicular to the optical axis, and the inclination angle of the focal plane is 0 degree. According to the lens column, spherical aberration and coma can be effectively corrected, and the imaging quality is improved; light scattering is reduced, the visual contrast at night and in a low-light environment is improved, and the visual experience of a patient is improved; the deviation tolerance of the implantation position is higher, and the operation difficulty and the risk of postoperative complications are reduced.

The invention provides an ultralow-swelling artificial cornea imitating natural cornea fiber arrangement and a preparation method, and belongs to the technical field of biomedical materials.The preparation method comprises the steps that I-type collagen is dissolved in a PBS buffer solution, a photofunctionalization modified active reagent is added for chemical modification, stirring is conducted in a dark place, freeze drying is conducted, and photofunctionalization collagen is obtained; dissolving the photo-functionalized collagen, and then preparing a collagen fiber membrane through electrostatic spinning; and performing phase change DLP 3D biological printing on the biological ink and the collagenous fiber membrane to obtain the keratoprosthesis with the collagenous fiber sandwich structure. Multi-layer arrangement is achieved through DLP 3D biological printing, the fiber trend of the natural cornea is simulated, and the mechanical anisotropy is remarkably improved; through a composite phase change DLP 3D printing mode of GelMA / HA-DN and photo-crosslinking collagenous fibers, the composite phase change DLP 3D printing material has good manufacturability, maintains high light transmittance and maintains high mechanical strength.

The invention belongs to the technical field of biomedical materials, and relates to a high-adhesion Janus artificial cornea as well as a preparation method and application thereof. The invention provides an artificial cornea, which is characterized in that a coating containing epsilon-PLL and 4-arm-PEG-NHS is distributed on a collagen skeleton EB-Col, and the functional group ratio of-NHS to-NH2 in the coating is 1: (0.6-1.4), and the molar ratio is 1: (0.6-1.4). The invention also relates to a corresponding high-adhesion Janus artificial cornea, and a preparation method and application thereof. The artificial cornea has instant and stable adhesion capability, gives consideration to optical transparency and mechanical and adhesion properties, can well adapt to the shape of a wound, is convenient to use, high in practical operability and mild in reaction, and is particularly suitable for being applied to cornea repair materials in the ophthalmology department, and the strong wet tissue adhesion property of the artificial cornea can adapt to a dynamic ocular surface moist environment.

The present invention discloses a one-time implant artificial cornea, belonging to the technical field of in-vivo prosthesis implantation. It solves at least the problems existing in the prior art, such as the need for donor corneas, multiple surgeries, large trauma, and inapplicability to patients with severe corneal blindness. The one-time implant artificial cornea includes a stent and a lens column. The lens column includes a lens column body and a rear disc coaxially arranged at the rear end face of the lens column body. The lens column is detachably connected to the stent coaxially. In the use state, the rear end of the lens column body behind the rear disc protrudes into the eye, the rear disc fits the posterior surface of the cornea, and the stent fits the anterior surface of the cornea. The stent is fixed on the corneal surface by using a suture method. At the same time, the stent and the rear disc clamp the cornea from the front and the back. The present invention also provides a method for using the one-time implant artificial cornea. It has the advantages of one-time implantation, no need for donor corneas, small trauma, and applicability to patients with severe corneal blindness, and has broad application prospects.

The invention relates to a collagen-based artificial cornea with a double-layer structure and capable of rapidly epithelizing and a preparation method thereof, and the preparation method comprises the following steps: adding collagen into an acetic acid solution, stirring and dissolving, and adding a plasticizer to obtain a high-concentration collagen solution and a low-concentration collagen solution; carrying out electrochemical deposition on the high-concentration collagen solution to obtain a collagen membrane I; carrying out photo-crosslinking on the collagen membrane I through strong ultraviolet irradiation; performing chemical crosslinking on the first collagen membrane to obtain a compact layer; a low-concentration collagen solution is taken and poured into the deposition tank, the compact layer is immersed, secondary electrochemical deposition is carried out, and a second collagen film is obtained on the compact layer; the second collagen membrane is subjected to secondary photo-crosslinking through weak ultraviolet irradiation, a loose layer is obtained, and the loose layer and the compact layer are combined to form the collagen-based artificial cornea which is of the double-layer structure and can be rapidly epithelized. The collagen-based artificial cornea with the double-layer structure and capable of being epithelialized rapidly has high light transmittance and meets clinical mechanical properties, the light transmittance at 600 nm is higher than 90%, the tensile strength of the collagen-based artificial cornea is higher than 1 MPa, and the elastic modulus of the collagen-based artificial cornea is higher than 10 MPa. The swelling rate is low, the tissue compression of the product on the autologous cornea can be avoided, the mechanical stimulation on the eye tissue is reduced, and the comfort after implantation is improved.

The invention discloses a corneal graft configuration measuring device based on a grating projection method and a use method, and relates to the technical field of corneal graft configuration measurement, the corneal graft configuration measuring device comprises a grating optical measuring component and an eyeball environment simulating component, the eyeball environment simulating component is an intraocular pressure bubbling instrument, the intraocular pressure bubbling instrument comprises a hydraulic cavity and an eyeball-imitating rigid support, and the hydraulic cavity is communicated with the eyeball-imitating rigid support. The eyeball-imitating rigid support is arranged at the top of the hydraulic cavity; the grating optical measuring component comprises a laser light source, a precise optical grating, a condensing lens, a transparent artificial cornea graft, a narrow-band filter and an industrial camera. According to the corneal graft configuration measuring device based on the grating projection method and the using method, the defects in the prior art are overcome by adopting a transmission-type measuring light path, the configuration evolution of the corneal graft in a standard intraocular pressure environment can be measured in a non-contact, real-time, high-precision and low-cost mode, the operation effect of corneal transplantation is simulated, and the measurement accuracy of the corneal graft is improved. And effective guidance is provided for optimization of a medical clinical corneal transplantation operation scheme.

The present disclosure relates to a support element (10) for an artificial cornea, the support element being disc-shaped and concentric with a central axis (P) and comprising: a central region (101) and a perimetral (102) region adjacent to a perimeter edge of the support element, the central region comprising a convex face (1011), a concave face (1012) opposed to each other and one central through hole (103) concentric with the central axis; wherein the perimetral region is flared, the surface of the support element is coated with silver, and at least the concave face of the support element comprises a peptide or a pharmaceutical salt thereof attached therein. The present disclosure also relates to an artificial cornea comprising the support element described.

This invention discloses an artificial corneal endothelial graft based on natural degradable proteins, its preparation method, and its application, relating to the field of medical implant materials technology. Currently, artificial corneal endothelial grafts for corneal endothelial decompensation are all non-degradable synthetic polymer materials. This invention uses a natural degradable material as the substrate to prepare the graft. A material preparation stock solution is obtained by mixing a silk fibroin solution extracted from a high-salt solution with a certain proportion of glycerol. The mixed solution is then spin-coated onto a mold using a gradient method, and dried to obtain a silk fibroin film. The obtained silk fibroin film is then circumcised and placed in a mold for hot-press steam fumigation. The resulting silk fibroin film can serve as an artificial corneal endothelial graft, providing a donor source for artificial corneas and possessing potential application value in corneal transplantation.

The present invention discloses a collagen-based artificial cornea with a double-layer structure and the ability to rapidly epithelialize, and a preparation method thereof, comprising the following steps: adding collagen to an acetic acid solution, stirring and dissolving, and adding a plasticizer to obtain a high-concentration collagen solution and a low-concentration collagen solution; electrochemically depositing the high-concentration collagen solution to obtain a first collagen film; photocrosslinking the first collagen film by irradiating the first collagen film with strong ultraviolet light; chemically crosslinking the first collagen film to obtain a compacting layer; pouring the low-concentration collagen solution into a sedimentation tank, immersing the compacting layer, and performing a second crosslinking process. The second electrochemical deposition method obtains a second collagen membrane on the compacting layer; the second collagen membrane is subjected to secondary photocrosslinking by weak ultraviolet irradiation to obtain a loose layer, and the loose layer and the compacting layer are combined to form the double-layer structure and the collagen-based artificial cornea capable of rapid epithelialization. The double-layer structure and the collagen-based artificial cornea capable of rapid epithelialization of the present invention have high light transmittance and mechanical properties that meet clinical requirements: the transmittance at 600nm is greater than 90%, the tensile strength is greater than 1Mpa, and the elastic modulus is greater than 10MPa. The low swelling rate can avoid the product from pressuring the autologous corneal tissue, reduce mechanical stimulation to the eye tissue, and improve comfort after implantation.

The application discloses a composite artificial cornea and a preparation method thereof. The composite artificial cornea comprises a mirror column and a biological skirt. An annular gap is arranged on the inner side edge of the biological skirt. The outer side edge of the mirror column is embedded into the inside of the biological skirt along the gap, and the outer side edge of the mirror column is fixed in the gap through casting liquid. The application has the beneficial effects that: by arranging the annular gap on the inner side edge of the biological skirt, then embedding the outer side edge of the mirror column into the inside of the biological skirt along the gap, a interference fit structure is formed, preliminary fixation is formed between the mirror column and the gap, and the outer side edge of the mirror column is fixed in the gap through the casting liquid, so that the casting liquid can permeate and fill the gap, the mirror column and the biological skirt are connected into an integrated whole, secondary fixation is formed, the connection strength of the composite artificial cornea is improved, and the risk of the mirror column falling off is reduced.

The invention discloses an artificial cornea applying Catalase (at) nHOFs nanoparticles, and belongs to the technical field of in-vivo prosthesis implantation. Frequent administration is not needed, long-acting ROS resistance can be achieved, and in addition, an existing artificial cornea structure does not need to be adjusted or changed. The part in the artificial cornea contact eye is provided with a Catalase (at) nHOFs nano coating; the Catalase (at) nHOFs nano coating is formed by encapsulating catalase by a hydrogen bond organic framework to prepare Catalase (at) nHOFs nano particles, and then the Catalase (at) nHOFs nano particles are adhered to the surface of the artificial cornea to form the Catalase (at) nHOFs nano coating.

The clamping type artificial cornea comprises a lens column body, a curved surface disc used for being attached to the rear surface of a cornea body is coaxially arranged on the outer ring face, close to the rear end, of the lens column body, a guide hole is formed in the curved surface disc, and the circumferential thickness of the curved surface disc is gradually increased from the center to the edge; the lens column body is detachably connected with a curved surface support matched with the curved surface disc, and the curved surface support is fixed in a sewing mode so as to be attached to the front surface of a cornea body. The curved surface disc is used for clamping a curved sample of a cornea body on the curved surface bracket; through cooperation of the curved-surface support and the curved-surface disc and the guide holes in the curved-surface disc, the cornea is clamped in a non-closed mode, the cornea can be clamped more conveniently, abrasion of the cornea body is reduced, the installation stability of the artificial cornea can be improved, the dead weight of the artificial cornea is reduced, and therefore it is guaranteed that the cornea can not be damaged along with the increase of wearing time. The installation position of the artificial cornea is not prone to deviation, and normal use is guaranteed.

The invention relates to the technical field of keratoprosthesis preparation, in particular to a biomimetic structure keratoprosthesis carrying a PRP microsphere slow release system and a preparation method of the biomimetic structure keratoprosthesis. PRP is encapsulated through PLGA microspheres, and stepped release of growth factors is achieved through degradation controllability of PLGA; the PRP-PLGA microspheres still keep a stable slow-release effect within 14 days in vitro, the problem that growth factors are rapidly inactivated due to direct application of traditional PRP is effectively solved, the treatment window period is remarkably prolonged, and continuous biological activity stimulation needed by cornea repair is met; by utilizing the degradation controllability of the PLGA, the stepped release of the growth factor is realized, so that the stable release effect of the PRP can be maintained for a long time after the artificial cornea is transplanted, the treatment window period after the artificial cornea is transplanted is prolonged, and the continuous biological activity stimulation required by cornea repair is met.

The application discloses a composite artificial cornea skirt preparation device and method, which comprises a turnover plate and driving units installed on both sides of the turnover plate, eye ball fixing cups are arranged on the upper end face of the turnover plate in a matrix mode, the upper end of the eye ball fixing cup is open and used for fixing an eye ball, a decellularization liquid tank is arranged below the turnover plate, the driving units are used for driving the turnover plate to overturn by 180 DEG and sink downwards until the cornea of the eye ball is completely immersed below the liquid level of the decellularization liquid tank, and the device further comprises a stroma plate layer cutting knife, a central stroma ring cutter, a stroma bulking device, a stroma plate layer tunnel knife and a peripheral stroma ring cutter. The application has the beneficial effects that the cleaning, material taking, decellularization, bulking and fixing of the corneal stroma are completed at one time, and the whole process is automatically, standardly and normatively produced, so that the decellularized corneal stroma skirt or a biological cornea is obtained.

The present invention provides a device for measuring large-field imaging of an artificial eye. A medium tank is provided at the upper end of the posterior chamber of the artificial eye. A light-transmitting hole is provided on the side wall of the posterior chamber of the artificial eye, and an artificial cornea and an artificial lens are respectively and hermetically installed in the light-transmitting hole. The medium tank is filled with a liquid medium. The input end of the artificial retina detector is located in the liquid medium, and the periphery of the artificial retina detector is fixedly installed at one end of a support rod. The other end of the support rod is installed on a displacement platform, and the displacement platform can drive the artificial retina detector to displace through the support rod. The lower end of the displacement platform is fixedly installed on the upper end of a substrate, and a support and a light source are respectively arranged at the lower end of the substrate. The device for measuring large-field imaging of an artificial eye according to the present invention can achieve the detection of large-field imaging of the artificial eye. The detector of the artificial retina is waterproofed, so it can be placed in the waterproof liquid of the artificial human eye, thereby approaching the actual imaging of the human eye to the greatest extent.

The present invention provides a recombinant human type II collagen fragment, the amino acid sequence is shown in SEQ ID NO.2, and the coding gene sequence is shown in SEQ ID NO.1. The present invention also provides an expression vector and Pichia pastoris engineering bacteria comprising the above-mentioned recombinant human type II collagen fragment coding gene. At the same time, the present invention also provides a preparation and application of the above-mentioned recombinant human type II collagen fragment. The recombinant human type II collagen fragment of the present invention not only has a significant effect on promoting the proliferation and migration of human chondrocytes, but also shows excellent effects of promoting cell migration and promoting adhesion in human immortalized epidermal cells and mouse embryonic fibroblasts, and can be widely used in artificial bones, artificial cartilage, artificial corneas, vitreous bodies, wound repair materials, supporting biomaterials, medical cosmetic surgery, cosmetics and other fields.

The application discloses an umbrella-shaped mirror column-based artificial cornea, which comprises a support and a mirror column connected with the support, wherein the support comprises a main body structure in a spherical surface structure, a middle part of the main body structure is provided with an opening, the mirror column is arranged in the opening, a micro-porous gap is formed on the circumferential surface of the main body structure, the top end surface of the mirror column is formed in an arc-shaped umbrella structure, and the bottom end surface of the mirror column is formed in a concave spherical surface structure. The application further discloses a preparation process of the artificial cornea. The artificial cornea is provided with the micro-porous gap on the circumferential surface of the main body structure, so that the healing tissue can pass through the micro-porous gap, the combination force between the support and the cornea is increased, and the stability is improved. In addition, the arc-shaped umbrella structure formed on the top end surface of the mirror column can prevent the growing tissue from covering the mirror surface of the mirror column in the later use of the patient, the light can enter the mirror column, the optical function can be normally played, and the occurrence of the anterior membrane complication is reduced.

The invention provides a method for constructing a tissue engineered cornea based on human amniotic epithelial stem cells and application, and belongs to the technical field of biology. The method comprises the following steps: 1) culturing human amniotic epithelial stem cells in a corneal epithelial cell induced differentiation culture medium to obtain human corneal epithelial cell-like cells; culturing the human amniotic epithelial stem cells in a corneal stroma cell induced differentiation culture medium to obtain corneal stroma cells; and 2) respectively inoculating the human corneal epithelial cell-like cells and corneal stroma cells obtained in the step 1) on the surface and inside of an acellular corneal stroma scaffold, and co-culturing for 5 days to construct the tissue engineered cornea. According to the method, the two corneal cells derived from the human amniotic epithelial stem cells are simultaneously inoculated to the human decellularized corneal stroma for the first time, the tissue-engineered cornea is successfully constructed, in addition, the corneal cells obtained through differentiation can be inoculated to various types of biological materials such as hydrogel, and the tissue-engineered cornea is obtained. Wider application possibility is provided for developing various novel artificial cornea products.

The present invention discloses an artificial cornea utilizing Catalase@nHOFs nanoparticles, belonging to the field of in vivo prosthetic implant technology. This method eliminates the need for frequent dosing and provides long-lasting protection against ROS. Furthermore, it eliminates the need to adjust or alter the existing artificial cornea structure. The portion of the artificial cornea that contacts the eye is coated with a Catalase@nHOFs nanocoating. The coating is prepared by encapsulating catalase within a hydrogen-bonded organic framework to form Catalase@nHOFs nanoparticles, which adhere to the surface of the artificial cornea to form the Catalase@nHOFs nanocoating.

To provide an artificial cornea suitable for surgical implantation.SOLUTION: An embodiment of an artificial cornea includes: an optical element that includes a body having an anterior side and a posterior side and an annular flange extending about the body; and a tissue integration skirt coupled to the optical element, the tissue integration skirt coupled to the optical element. The anterior side of the body includes an anterior optical surface and the posterior side of the body includes a posterior optical surface. The tissue integration skirt is configured to promote tissue ingrowth, and the tissue integration skirt is coupled to the optical element such that at least a portion of a periphery of the annular flange defined between the anterior and posterior sides of the optical element is covered by the tissue integration skirt. Also described are methods for implanting an artificial cornea of the present disclosure, the methods including providing the artificial cornea, removing a section of corneal tissue from the patient's cornea to form a tissue bed of existing tissue to which the artificial cornea can be affixed, implanting the artificial cornea such that the posterior side of the artificial cornea is suspended above the interior of the eye, and mechanically affixing the implanted artificial cornea to the existing corneal tissue of the tissue bed.SELECTED DRAWING: Figure 4

The invention discloses an artificial cornea, comprising a mirror column and a bracket. The mirror column comprises an upper mirror column section and a lower mirror column section. The upper mirror column section and the lower mirror column section are separate components. Both the upper mirror column section and the lower mirror column section are mounted on the bracket, and the upper mirror column section and the bracket are detachably connected. The upper mirror column section is arranged on the upper part of the lower mirror column section, and the bottom of the upper mirror column section abuts against the top of the lower mirror column section. The top of the upper mirror column section is an outer convex surface, and the bottom of the lower mirror column section is an inner concave surface.

The application discloses a kind of methacrylic acid succinic acid double ester starch and its preparation method and application in corneal defect in situ filling agent, belong to the technical field of biomedical materials.The methacrylic acid succinic acid double ester starch is grafted carboxylation treatment to starch, then graft double bond is prepared.The corneal defect in situ filling agent of the application is composed of methacrylic acid succinic acid double ester starch (StacMA), methacrylated gelatin (GelMA), photo initiator and solvent.The natural biological macromolecule based corneal filling agent of starch and gelatin in the application has good biocompatibility, excellent light response adhesion characteristics and excellent mechanical properties, and the in-situ photocrosslinking of hydrogel is realized by ultraviolet irradiation, while excellent mechanical properties and strong adhesion capacity are generated, to realize the stable adhesion of in-situ forming artificial cornea on corneal implant bed, promote the regeneration of damaged corneal natural structure and the reconstruction of visual function.

The present invention discloses a humanoid eye-mimicking artificial cornea for single implantation, belonging to the technical field of in-vivo prosthesis implantation. It solves at least the problem that the existing artificial corneas cannot simulate the appearance of real eyes. The artificial cornea includes a lens column, a stent and a cosmetic disc; wherein, the lens column includes a lens column body and a rear disc coaxially arranged at a position close to the rear end face of the lens column body; the cosmetic disc is coaxially arranged at the front end of the lens column body; the stent is coaxially and movably arranged on the lens column body between the rear disc and the cosmetic disc; in the use state, the stent and the rear disc clamp the cornea one in front and the other behind; the cosmetic disc has a central opening and has an iris texture area and a sclera texture area; an iris texture layer is formed in the iris texture area, and a mimic conjunctival vascular texture layer is formed in the sclera texture area. The present invention also provides a method for using the humanoid eye-mimicking artificial cornea for single implantation.

The present invention relates to a method for manufacturing an artificial cornea exhibiting excellent physical properties by stably adhering an interface between an optical unit and a support unit by means of an artificial cornea mold and UV dose modulation. According to the present invention, an optical unit is manufactured for one to four hours using low-dose UV in an artificial cornea mold so as to stably adhere an interface between an optical unit and a support unit, thereby manufacturing an artificial cornea in which the optical unit and the support unit are integrated with each other. The artificial cornea manufactured by the manufacturing method has a consistent spherical shape, which is convenient for centering and increases surgical success rates during surgery, the central part of the optical unit exhibits uniform and excellent physical properties to further increase physical safety in the human body, and the boundary surface between the optical unit and the support unit exhibits about 100 times more excellent physical properties compared to existing intraocular pressure, thus allowing early prevention of the optical unit and the support unit separating due to intraocular pressure in the human body. In addition, the manufacturing method can improve the appearance of a patient implanted with an artificial cornea and reduce complications and side effects caused by adhesive material.

The utility model discloses a bracket for an artificial cornea, which is used for improving the bonding strength between the bracket and a chemical central area of the artificial cornea and simultaneously improving the biocompatibility between the bracket and a maternal cornea tissue. The support is a medical metal support and comprises a support body, a central mounting hole penetrating through the support body and a connecting ring coaxially clamped into the central mounting hole from top to bottom, and internal threads are formed in the inner wall of the connecting ring. A hydroxyapatite layer is formed on the surface of the stent body; the material of the connecting ring is the same as or similar to that of a lens column of the artificial cornea; the connecting ring is of a hollow stepped shaft structure, an annular clamping groove is formed in the corner of a stepped shaft, and in the assembled state, the stent body is clamped into the annular clamping groove, so that the connecting ring is prevented from shifting or falling off in the operation.

The invention provides an artificial cornea and a preparation method thereof, and relates to the field of ophthalmology medical instruments. The preparation method of the artificial cornea comprises the following steps that an acidic electrolyte is provided, the acidic electrolyte contains collagen, chitosan and hydrogen peroxide, the collagen in the acidic electrolyte is pre-treated collagen, the pre-treatment comprises first treatment and second treatment, the first treatment comprises freeze-drying treatment on collagen liquid, the second treatment comprises freeze-drying treatment on the collagen liquid, and the third treatment comprises freeze-drying treatment on the collagen liquid; the collagen solution contains a phosphate solution, and collagen is dissolved in the phosphate solution; the second treatment comprises the step of carrying out reverse osmosis treatment on the collagen subjected to the first treatment by utilizing an acid solution; the negative electrode is used as a working electrode, and the working electrode is provided with a working surface with a spherical convex surface or a spherical concave surface; the working electrode and the positive electrode are inserted into the acidic electrolyte and powered on to perform electrochemical reaction, and a gel film is formed on the working surface of the working electrode. The artificial cornea prepared by the preparation method is relatively high in light transmittance and relatively good in mechanical strength.

The present invention discloses a manufacturing method of an artificial cornea for one-time implantation, belonging to the technical field of in-vivo prosthesis implantation. It solves at least the problems existing in the prior art, such as the need for donor corneas, multiple surgeries, large trauma and inapplicability to patients with severe eye diseases. The manufacturing method includes: manufacturing a lens column, which sequentially includes a first threaded section, a second threaded section, an optical axis section, a rear disc and a rear convex section from the front end to the rear end; manufacturing a stent, which includes a stent body and a connecting ring; the stent body is made of metal and is formed by stamping; manufacturing a cosmetic disc, the material of the cosmetic disc is the same as that of the lens column and is formed by injection molding, the cosmetic disc has a central connection hole, and the inner wall of the central connection hole has an internal thread that is screwed with the first threaded section; assembling the lens column, the stent and the cosmetic disc, specifically including: snapping the connecting ring into the central mounting hole of the stent body from top to bottom, and making the stent body snap into the annular limiting groove of the connecting ring to complete the assembly of the stent.

The present invention provides a type II collar button-shaped artificial cornea, which includes a front plate with an optical lens cylinder. A skirt is annularly arranged on the outer side surface of the optical lens cylinder. A gasket is also annularly arranged on the outer side surface of the optical lens cylinder. The gasket is located between the front plate and the skirt, and the gasket is attached to the skirt. The type II collar button-shaped artificial cornea of the present invention can better clamp the eyelids, reduce the shedding rate of the artificial cornea, and can be better fixed in the eye.

The application provides a method for constructing a tissue-engineered cornea based on human amniotic epithelial stem cells and application thereof, and belongs to the technical field of biotechnology. The method comprises the following steps: 1) culturing human amniotic epithelial stem cells in a corneal epithelial cell induction and differentiation culture medium to obtain human corneal epithelial cell-like cells; culturing the human amniotic epithelial stem cells in a corneal stromal cell induction and differentiation culture medium to obtain corneal stromal cells; 2) inoculating the human corneal epithelial cell-like cells and the corneal stromal cells obtained in step 1) on the surface and inside of a decellularized corneal stromal scaffold respectively, and co-culturing for 5 days to construct a tissue-engineered cornea. The application successfully constructs a tissue-engineered cornea by inoculating two kinds of corneal cells derived from human amniotic epithelial stem cells on a human decellularized corneal stromal at the same time for the first time. In addition, the obtained corneal cells can be inoculated on various types of biomaterials such as hydrogels, thereby providing a wider application possibility for developing various types of artificial cornea products.