112 results about "Collagen type" patented technology

The present invention relates to the field of biomedical materials, particularly to a novel composite collagen material for wound repair, and more specifically to a method for preparing a novel composite collagen material comprising recombinant humanized collagen type 21 (ColpepA1 21) and its use in wound repair. The present invention utilizes ColpepA1 21 to significantly promote key biological reactions in the wound healing process, including accelerating cell migration, enhancing skin hydration and barrier function, and improving the stability and elasticity of skin structure. The resulting novel composite collagen material effectively promotes wound healing and cell regeneration, alleviating oxidative damage.

The invention provides recombinant IV type collagen and application thereof, and relates to the technical field of protein engineering. In the invention, an amino acid residue repetitive unit with good biological activity and stability in the human-derived IV-type collagen is found, and the amino acid residue repetitive unit is used as a core for repeating and constructing the recombinant IV-type collagen, so that the novel human-derived recombinant IV-type collagen with biological activity is obtained. The human-derived recombinant IV-type collagen provided by the invention is a small-molecular-weight protein, is easy to absorb by a human body, is good in safety, can remarkably promote secretion of collagen, also has the effects of promoting cell adhesion and cell migration, and has a good application prospect in the aspects of basement membrane repair and remodeling. A new functional component is provided for products for repairing and tightening the skin, resisting aging, resisting wrinkles, preserving moisture and the like.

The invention relates to a recombinant I-type humanized collagen with crosslinking property as well as a preparation method and application thereof, and belongs to the technical field of recombinant proteins. The invention relates to recombinant I-type humanized collagen with crosslinking property. The amino acid sequence of the recombinant I-type humanized collagen comprises an amino acid sequence as shown in SEQ ID No.1 or a derivative amino acid sequence with the sequence identity of more than 90% with the sequence as shown in SEQ ID No.1; the recombinant I-type humanized collagen has a triple helix structure. A complete triple helix structure can be efficiently expressed, and the hydrogel has efficient cross-linking capability, so that the hydrogel can be efficiently and stably formed through cross-linking.

The invention provides a recombinant III-type humanized collagen gel based on a macromolecular crowding effect as well as a preparation method and application of the recombinant III-type humanized collagen gel. The method comprises the following steps: firstly, preparing an rhCol III solution system; then preparing a macromolecular crowding bath containing dextran 70, polyethylene glycol 8000 and ascorbic acid; then adding an rhCol III solution into the macromolecular crowding bath to form an rhCol III microgel; and finally, constructing a 3D printing molecular exclusion mold system which takes agarose as a substrate and contains a functional additive and a temperature-sensitive polymer as a temperature-sensitive release and spatial constraint component, so as to realize directional construction of the rhCol III microgel. According to the method disclosed by the invention, second-level physical gelling (lt; 15s) of rhCol is realized, and a physiological microenvironment supporting cell proliferation is constructed. The recombinant III-type humanized collagen gel prepared by the method can be used for preparing collagen microgel balls, collagen bundles, 3D (three-dimensional) bio-printing products and the like, and is wide in application range.

The present invention relates to the field of biomaterials, specifically a collagen material comprising recombinant humanized collagen type 21 (ColpepA1 21) and its application in facial augmentation. This invention utilizes collagen to not only provide an effective solution to skin aging but also promotes the natural synthesis of collagen within the skin, optimizing skin hydration and barrier function, and enhancing the skin's self-repair capacity. The resulting collagen material is a facial augmentation material. Through local injection or external application, it rapidly smoothes and firms the facial skin, while also improving overall skin health with long-term use.

The invention relates to the technical field of synthetic biology, in particular to polypeptide with a triple helix structure, recombinant XII type humanized collagen and application of the recombinant XII type humanized collagen. The recombinant XII type humanized collagen is successfully expressed and prepared, has a triple-helix structure and good biological activity including promotion of cell proliferation activity, cell adhesion activity and inhibition of MMP-1, is used as a biological material derived from a human body, does not generate immunological rejection and anaphylactic reaction when applied to the human body, and has a good application prospect. The composition can be used for medical or non-medical application of a plurality of tissues and organs of a human body, filling, compatibilizing or repairing, and promotion of skin compactness or wrinkle resistance and other scenes.

A method for preparing nondenatured collagen type II for alleviating joint pain includes pulverizing a cartilage raw material and defatting to obtain a total protein crude extract; adding anhydrous ethanol to the total protein crude extract, soaking, stirring, and centrifuging to obtain an anhydrous ethanol insoluble material; dissolving the anhydrous ethanol insoluble material in water, adjusting pH to acidic, stirring and centrifuging to obtain an acid-insoluble material; dissolving the acid-insoluble material in water, performing enzymatic hydrolysis and centrifuging to obtain an enzymatic hydrolysis precipitate; dissolving the enzymatic hydrolysis precipitate in water, followed by performing co-fermentation, and centrifuging to obtain a fermentation precipitate; and adding a water activity regulator to the fermentation precipitate, and then drying to obtain the nondenatured collagen type II. The content of protein, hydroxyproline and nondenatured collagen type II in the prepared nondenatured collagen type II is high, and the shelf life is prolonged.

The invention relates to the technical field of recombinant proteins, and relates to a recombinant XII type collagen as well as a preparation method and application thereof. The recombinant XII type collagen is formed by connecting 5-30 repetitive units in series, and the amino acid sequence of each repetitive unit is as shown in SEQ ID NO: 1. The nucleic acid molecule is used for coding the recombinant XII type collagen; a vector comprises the nucleic acid molecule. According to the invention, a nucleotide sequence for coding the amino acid sequence is inserted into a vector to obtain a recombinant plasmid vector, then the recombinant plasmid vector is introduced into a host cell for culture, and then separation and purification are carried out to obtain the recombinant XII type collagen. The recombinant XII type collagen provided by the invention is easy to obtain, can realize efficient secretory expression, and meets the requirements of industrial production; the biological activity is good, and the proliferation and migration of human immortalized cartilage cells can be promoted.

The invention relates to the technical field of biology, and particularly discloses polypeptide, recombinant XIX type humanized collagen and application of the recombinant XIX type humanized collagen. The amino acid sequence of the polypeptide comprises n repetitive units; the repetitive unit comprises any one of the following amino acid sequences or variant sequences thereof: (1) an amino acid sequence as shown in any one of SEQ ID NO.1-2; (2) an amino acid sequence having at least 50%, 60%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% sequence identity with the amino acid sequence as shown in any one of SEQ ID NO.1-2; (3) a variant sequence obtained by performing mutation of one or more amino acid residues on the amino acid sequence as shown in any one of SEQ ID NO.1-2; wherein n is an integer greater than or equal to 1. The polypeptide provided by the invention has a collagen triple helix structure, has good cell adhesion activity, can carry out functions of human collagen, is free of immunological rejection and anaphylactic reaction, and can be used in various fields of cartilage repair, biological dressing, tissue filling and the like.

The present disclosure relates to amino acid and plant extract compositions and uses thereof, and also to joint care compositions comprising the amino acid and plant extract. The composition disclosed by the invention can increase bone hardness, prevent inflammatory response, relieve arthralgia and / or improve joint flexibility, so that systematic support is provided for bone and joint health.

The invention discloses a recombinant II-type collagen with a cartilage filling effect and application of the recombinant II-type collagen. The amino acid sequence of the recombinant II type collagen is as shown in SEQ ID NO: 1. By intercepting amino acids from the 722nd site to the 955th site of the natural II-type collagen, the recombinant II-type collagen which is easy to produce and has a cartilage filling effect superior to that of the full-length II-type collagen can be obtained.

The present application provides gastrodia elata (Blume) alkaloids PFT201A, PFT201B and PFT203 and a preparation method thereof, and application thereof in the fields of cosmetics and medicine. Gastrodia elata Gastrodia elata (Blume) alkaloids PFT201A, PFT201B and PFT203 significantly promote the secretion of collagen type II in human dermal fibroblasts-adult (HDFa) at an extremely low concentration (0.1 μ M), and have no cytotoxicity at the concentration. These compounds can be used for preparing anti-aging drugs or skin care products.

This patent application discloses a recombinant human type II collagen, its preparation method, and its uses. The recombinant human type II collagen of this patent application is a truncated protein of natural human type II collagen, and its amino acid sequence is shown in SEQ ID No: 1. The recombinant human type II collagen of this invention can be expressed in commercial expression systems such as the commercial Pichia pastoris expression system with high expression yields. Furthermore, its tested biological activities (promoting cell proliferation and wound healing) are significantly superior to those of natural human type II collagen, and also significantly superior to other stages of natural human type II collagen.

The invention relates to the technical field of biological agents, and provides a recombinant III-type collagen / platycladus orientalis essential oil nanoemulsion which is prepared from the following raw materials in percentage by weight: 15-35% of recombinant III-type collagen, 1-10% of platycladus orientalis essential oil and the balance of pure water. The invention also provides a preparation method of the nanoemulsion, which comprises the following steps: weighing the recombinant III collagen and the pure water according to the weight percentage, mixing and dissolving, then adding the platycladus orientalis essential oil, stirring and uniformly mixing to form primary emulsion, and carrying out high-speed shearing for 3-9 minutes by using a high-speed shearing emulsifying machine at the rotating speed of 8000r / min, so as to obtain the recombinant III collagen nanoemulsion. The recombinant III type collagen / platycladus orientalis essential oil nanoemulsion is obtained. The prepared oriental arborvitae essential oil nanoemulsion is uniform in size, high in stability and easy to store. The recombinant III type collagen has good biological activity and degradability, and can effectively reduce the irritation. The prepared nanoemulsion can significantly improve the antibacterial property of the platycladus orientalis essential oil nanoemulsion, and has a good prospect in application to bacterial infectious wounds.

This invention provides the application of type IV collagen and its encoding gene in the prognostic assessment and treatment of nasopharyngeal carcinoma (NPC). Research has shown that high expression of type IV collagen and its encoding gene COL4A1 is significantly associated with lower T-cell infiltration in patients with recurrent NPC; compared to NPC patients with low expression of type IV collagen, those with high expression have worse LRRFS and OS; and the expression level of the type IV collagen encoding gene COL4A1 is significantly associated with worse PFS in NPC patients. Therefore, type IV collagen and its encoding gene COL4A1 can serve as specific prognostic biomarkers for assessing the prognosis of NPC patients. Furthermore, inhibiting the expression of type IV collagen can effectively inhibit the growth of NPC; therefore, agents that inhibit type IV collagen expression can be used as drugs to inhibit immune escape in NPC and for the treatment of NPC.

The present invention discloses the use of Avn Anthramides C (Avn C) as an inducer of chondrocytic differentiation in Mesenchymal Stem Cells (MSCs), providing a promising approach for regenerative cartilage therapy. By employing Avn C treatment, MSCs exhibit enhanced chondrocytic differentiation and elevated expression of key specific chondrogenic extracellular matrix (ECM) factors, notably Collagen Type II (COL2A1). Furthermore, when Avn C is used in combination with Chondroitin Sulfate (CS), a synergistic effect is observed, leading to upregulation of the transcriptional activity state of SOX9, a key regulator of chondrogenesis. The disclosed invention shows significant improvements in mRNA gene expression (ACAN, LRP1, SOX9), cellular protein levels (COL2A1, SOX9, LRP1), and functional outcomes, as evidenced by the secretion of extracellular matrix proteins (FSTL-1, BGH3, CO1A1, CO1A2, and CO3A1).

The application discloses a kind of antibody for detecting collagen type IV alpha 3, detection kit and its application, belong to immunodetection technical field.Wherein, the antibody for detecting collagen type IV alpha 3 is COL4A3 monoclonal antibody, the COL4A3 monoclonal antibody can specifically recognize collagen type IV alpha 3, such as the protein fragment shown in SEQ ID NO:9.The application further discloses an antibody combination comprising the COL4A3 monoclonal antibody.The application further discloses the application of the COL4A3 monoclonal antibody or antibody combination in the preparation of collagen type IV detection kit and the kit prepared by the same.The kit of the application has very great clinical application value for detecting Alport syndrome with high specificity and strong sensitivity.

The application provides a composition containing curcuma and type II collagen-containing cartilage powder and application thereof in joint maintenance, and relates to the field of food technology. The application provides a composition containing milk mineral salt, curcuma, type II collagen-containing cartilage powder, enzymatic bone powder, N-acetyl glucosamine and black pepper extract, which can significantly repair joint damage, significantly reduce cartilage damage indexes such as CTX-II and COMP, and can be used for preparing a joint maintenance medicine.

This invention relates to the field of biotechnology, specifically disclosing a novel polypeptide, recombinant XIX type humanized collagen, and their applications. This invention provides a novel polypeptide with good cell adhesion activity and the ability to promote cell proliferation. The amino acid sequence of the polypeptide comprises n repeating units; each repeating unit comprises any one of the following (1)-(3) amino acid sequences or their variants: (1) the amino acid sequence shown in SEQ ID NO.1; (2) an amino acid sequence having at least 50%, 60%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% sequence identity with the amino acid sequence shown in SEQ ID NO.1; (3) a variant sequence obtained by mutating one or more amino acid residues of the amino acid sequence shown in SEQ ID NO.1; wherein n ≥ 1 and is an integer. The polypeptide of this invention can be used in various fields such as promoting skin barrier repair, wound healing, and epidermal renewal.

The present application relates to the field of cosmetics, in particular to an endogenous antioxidant anti-wrinkle composition, a preparation method and application thereof, the composition comprises acetyl tetrapeptide-5, CycloRetin, collagen type 21, acetyl hexapeptide-8 and vitisins, the composition can promote the synthesis of collagen in the extracellular matrix of the dermis of the skin, and can improve the tightening and anti-wrinkle effect, human test proves that the tightening and anti-wrinkle effect of the composition is the best.

The invention discloses haliotis discus hannai I-type collagen peptide as well as a screening method and application thereof, and belongs to the technical field of bioactive peptides. The peptide sequences of the haliotis discus hannai I type collagen peptide are GAAGDK and DSQSAR; the screening method specifically comprises the following steps: (1) virtual enzymolysis of the haliotis discus hannai I-type collagen; (2) screening the bioactive peptide; and (3) molecular docking. According to the invention, through rational design and a virtual screening strategy, a human gastrointestinal tract digestion process is simulated to carry out targeted virtual enzymolysis, a multi-target molecular docking technology is combined, brand-new bifunctional peptides GAAGDK and DSQSAR are accurately predicted and screened from a specific collagen sequence, and aiming at a dual-action mechanism of AGE-RAGE axis and oxidative stress, the application has the advantages of high sensitivity, high sensitivity and high stability. The composition has remarkable synergistic interaction potential in the aspects of preventing diabetic complications and delaying skin and body senescence, and a brand new core raw material is provided for developing next-generation anti-saccharification functional food and skin care products.

The application relates to a recombinant type I collagen and application thereof, which utilizes the characteristics of Pichia pastoris capable of secreting and expressing recombinant proteins, secretes the recombinant type I collagen on the culture medium supernatant, and reduces the difficulty of protein purification. In addition, the integrin site RGD is inserted into the collagen to form a recombinant type I collagen with high stability and high expression. According to the relative proliferation of cells and the relative adhesion experiment of cells, it can be seen that the recombinant type I collagen has the effects of promoting the proliferation of skin fibroblasts, improving the skin, promoting wound healing, promoting the adhesion of fibroblasts in the skin layer and the like. Therefore, the recombinant type I collagen has great potential applications in the fields of skin care and beauty, medical treatment and the like.

The present disclosure provides a recombinant humanized collagen VIII, which is a triple helix structure, has good cell adhesion activity, and is applied to human body without immune rejection and allergic reaction.

The invention belongs to the technical field of protein engineering, and discloses a humanized collagen XVII type polypeptide as well as a preparation method and application thereof. The amino acid sequence of the humanized collagen XVII type polypeptide is as shown in SEQ ID NO.1, and the polynucleotide sequence for coding the humanized collagen XVII type polypeptide is as shown in SEQ ID NO.2. The invention also provides application of the polypeptide in preparation of a protein product with functions of preventing hair loss and growing hair and / or repairing, resisting wrinkles and tightening skin. The application proves that the humanized collagen XVII type polypeptide can promote proliferation of hair papilla cells, keratinocytes and fibroblasts and promote up-regulation of expression of beta-Catenin signal channel protein closely related to hair growth in the hair papilla cells; the up-regulation expression of fibroblast type III collagen, type IV collagen, type VII collagen, fibronectin and elastin is promoted, so that the polypeptide is proved to have the effects of preventing hair loss and growing hair and / or repairing, resisting wrinkles and tightening skin.

The present invention relates to methods of immunoassay for detecting a biomarker which is an MMP-cleaved fragment of collagen type-I and its use in detecting and / or monitoring diseases such as kidney disease and diseases of the cardiovascular system including the heart.

The invention discloses recombinant III-type humanized collagen gel and a preparation process thereof, and belongs to the field of biological medicines and high polymer materials. The gel aims at solving the problems that in the prior art, collagen gel is weak in mechanical property, short in maintaining time and single in biological activity. The key point of the technical scheme is that the gel has a double-network interpenetrating structure, and the structure comprises a first network formed by recombinant III-type humanized collagen cross-linked by an enzyme method and a second network which is physically interpenetrated with the first network and is formed by thermo-sensitive macromolecules (such as poloxamer). Preferably, slow-release nanoparticles loaded with growth factors (such as bFGF) are also loaded in the gel so as to actively induce tissue regeneration. According to the preparation process, the components are mixed at low temperature, and a first network and a second network are sequentially formed through an enzymatic reaction and temperature response two-step method. The gel disclosed by the invention has excellent mechanical supporting property, long-acting stability and active biological activity, and is suitable for the fields of medical beauty filling and tissue repair.

This invention relates to the fields of molecular cloning and protein expression, and particularly to a Pichia pastoris engineered strain expressing full-length human type III collagen and its applications. The engineered Pichia pastoris strain is obtained by cloning the enzymatically digested human type III collagen gene sequence into an expression vector to obtain a recombinant expression vector, which is then transformed into Pichia pastoris. This engineered Pichia pastoris strain was deposited at the China General Microbiological Culture Collection Center (CGMCC) on November 6, 2024, with accession number CGMCC NO.32510. The Pichia pastoris engineered strain obtained by this invention can efficiently produce the desired human type III collagen through fermentation, exhibiting excellent characteristics such as high yield and absence of endotoxins.

The application discloses a silk material with a cell proliferation function and application thereof, the functional material is expressed by a human type I collagen truncated fragment in a middle silk gland of a domestic silkworm, a sericin promoter is used as a tissue-specific promoter to drive specific expression of an exogenous gene in the middle silk gland, and finally the exogenous gene is combined with sericin protein and is secreted to outside of the body, the exogenous protein can be separated after a certain treatment of a cocoon, and the expression product can keep biological activity. The silk material is used to prepare a hydrogel, has a function of promoting wound healing, and can be applied to aspects of promoting skin regeneration and repair, bone graft substitutes, cartilage reconstruction, artificial skin and the like.

The invention discloses a water-based skin care product containing type IV collagen and a preparation method of the water-based skin care product. The water-based skin care product comprises the recombinant collagen skin care composition and a water-based matrix. The recombinant collagen skin care composition comprises a recombinant IV type collagen, a GLP tripeptide, a PGD tripeptide, a recombinant III type collagen, a recombinant I type collagen and a recombinant human-like collagen according to a specific ratio. According to the invention, the recombinant collagen composition is creatively applied to a water-based skin care product to maintain the collagen of the whole skin layer, so that the effect of comprehensively promoting skin barrier repair is achieved.

Provided is a method for preparing a recombinant human collagen. The method comprises: constructing a genetically engineered bacterium which expresses a prolyl hydroxylase and a recombinant human collagen; culturing the genetically engineered bacterium and performing digestion treatment by means of pepsin to obtain a recombinant human collagen type I or type III. The prolyl hydroxylase comprises P4Ha2, P4Hb and vP4H, and by means of controlling the ratio of the copy numbers of the genes thereof, the recombinant human collagen and a natural human collagen are consistent in hydroxylation modification. Also provided is a large-scale production method for the recombinant human collagen. The prepared recombinant human collagen has completely the same sequence composition and length and substantially the same hydroxyproline content as the natural human collagen, and also has a stable full-length triple helix structure and biological activity without immunogenicity and potential risk of viruses.