80 results about "Collagen type" patented technology

The invention provides recombinant IV type collagen and application thereof, and relates to the technical field of protein engineering. In the invention, an amino acid residue repetitive unit with good biological activity and stability in the human-derived IV-type collagen is found, and the amino acid residue repetitive unit is used as a core for repeating and constructing the recombinant IV-type collagen, so that the novel human-derived recombinant IV-type collagen with biological activity is obtained. The human-derived recombinant IV-type collagen provided by the invention is a small-molecular-weight protein, is easy to absorb by a human body, is good in safety, can remarkably promote secretion of collagen, also has the effects of promoting cell adhesion and cell migration, and has a good application prospect in the aspects of basement membrane repair and remodeling. A new functional component is provided for products for repairing and tightening the skin, resisting aging, resisting wrinkles, preserving moisture and the like.

The invention relates to a recombinant I-type humanized collagen with crosslinking property as well as a preparation method and application thereof, and belongs to the technical field of recombinant proteins. The invention relates to recombinant I-type humanized collagen with crosslinking property. The amino acid sequence of the recombinant I-type humanized collagen comprises an amino acid sequence as shown in SEQ ID No.1 or a derivative amino acid sequence with the sequence identity of more than 90% with the sequence as shown in SEQ ID No.1; the recombinant I-type humanized collagen has a triple helix structure. A complete triple helix structure can be efficiently expressed, and the hydrogel has efficient cross-linking capability, so that the hydrogel can be efficiently and stably formed through cross-linking.

The invention relates to the technical field of synthetic biology, in particular to polypeptide with a triple helix structure, recombinant XII type humanized collagen and application of the recombinant XII type humanized collagen. The recombinant XII type humanized collagen is successfully expressed and prepared, has a triple-helix structure and good biological activity including promotion of cell proliferation activity, cell adhesion activity and inhibition of MMP-1, is used as a biological material derived from a human body, does not generate immunological rejection and anaphylactic reaction when applied to the human body, and has a good application prospect. The composition can be used for medical or non-medical application of a plurality of tissues and organs of a human body, filling, compatibilizing or repairing, and promotion of skin compactness or wrinkle resistance and other scenes.

The invention discloses a recombinant II-type collagen with a cartilage filling effect and application of the recombinant II-type collagen. The amino acid sequence of the recombinant II type collagen is as shown in SEQ ID NO: 1. By intercepting amino acids from the 722nd site to the 955th site of the natural II-type collagen, the recombinant II-type collagen which is easy to produce and has a cartilage filling effect superior to that of the full-length II-type collagen can be obtained.

This patent application discloses a recombinant human type II collagen, its preparation method, and its uses. The recombinant human type II collagen of this patent application is a truncated protein of natural human type II collagen, and its amino acid sequence is shown in SEQ ID No: 1. The recombinant human type II collagen of this invention can be expressed in commercial expression systems such as the commercial Pichia pastoris expression system with high expression yields. Furthermore, its tested biological activities (promoting cell proliferation and wound healing) are significantly superior to those of natural human type II collagen, and also significantly superior to other stages of natural human type II collagen.

This invention provides the application of type IV collagen and its encoding gene in the prognostic assessment and treatment of nasopharyngeal carcinoma (NPC). Research has shown that high expression of type IV collagen and its encoding gene COL4A1 is significantly associated with lower T-cell infiltration in patients with recurrent NPC; compared to NPC patients with low expression of type IV collagen, those with high expression have worse LRRFS and OS; and the expression level of the type IV collagen encoding gene COL4A1 is significantly associated with worse PFS in NPC patients. Therefore, type IV collagen and its encoding gene COL4A1 can serve as specific prognostic biomarkers for assessing the prognosis of NPC patients. Furthermore, inhibiting the expression of type IV collagen can effectively inhibit the growth of NPC; therefore, agents that inhibit type IV collagen expression can be used as drugs to inhibit immune escape in NPC and for the treatment of NPC.

The present invention discloses the use of Avn Anthramides C (Avn C) as an inducer of chondrocytic differentiation in Mesenchymal Stem Cells (MSCs), providing a promising approach for regenerative cartilage therapy. By employing Avn C treatment, MSCs exhibit enhanced chondrocytic differentiation and elevated expression of key specific chondrogenic extracellular matrix (ECM) factors, notably Collagen Type II (COL2A1). Furthermore, when Avn C is used in combination with Chondroitin Sulfate (CS), a synergistic effect is observed, leading to upregulation of the transcriptional activity state of SOX9, a key regulator of chondrogenesis. The disclosed invention shows significant improvements in mRNA gene expression (ACAN, LRP1, SOX9), cellular protein levels (COL2A1, SOX9, LRP1), and functional outcomes, as evidenced by the secretion of extracellular matrix proteins (FSTL-1, BGH3, CO1A1, CO1A2, and CO3A1).

The application provides a composition containing curcuma and type II collagen-containing cartilage powder and application thereof in joint maintenance, and relates to the field of food technology. The application provides a composition containing milk mineral salt, curcuma, type II collagen-containing cartilage powder, enzymatic bone powder, N-acetyl glucosamine and black pepper extract, which can significantly repair joint damage, significantly reduce cartilage damage indexes such as CTX-II and COMP, and can be used for preparing a joint maintenance medicine.

This invention relates to the field of biotechnology, specifically disclosing a novel polypeptide, recombinant XIX type humanized collagen, and their applications. This invention provides a novel polypeptide with good cell adhesion activity and the ability to promote cell proliferation. The amino acid sequence of the polypeptide comprises n repeating units; each repeating unit comprises any one of the following (1)-(3) amino acid sequences or their variants: (1) the amino acid sequence shown in SEQ ID NO.1; (2) an amino acid sequence having at least 50%, 60%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% sequence identity with the amino acid sequence shown in SEQ ID NO.1; (3) a variant sequence obtained by mutating one or more amino acid residues of the amino acid sequence shown in SEQ ID NO.1; wherein n ≥ 1 and is an integer. The polypeptide of this invention can be used in various fields such as promoting skin barrier repair, wound healing, and epidermal renewal.

The present application relates to the field of cosmetics, in particular to an endogenous antioxidant anti-wrinkle composition, a preparation method and application thereof, the composition comprises acetyl tetrapeptide-5, CycloRetin, collagen type 21, acetyl hexapeptide-8 and vitisins, the composition can promote the synthesis of collagen in the extracellular matrix of the dermis of the skin, and can improve the tightening and anti-wrinkle effect, human test proves that the tightening and anti-wrinkle effect of the composition is the best.

The invention discloses haliotis discus hannai I-type collagen peptide as well as a screening method and application thereof, and belongs to the technical field of bioactive peptides. The peptide sequences of the haliotis discus hannai I type collagen peptide are GAAGDK and DSQSAR; the screening method specifically comprises the following steps: (1) virtual enzymolysis of the haliotis discus hannai I-type collagen; (2) screening the bioactive peptide; and (3) molecular docking. According to the invention, through rational design and a virtual screening strategy, a human gastrointestinal tract digestion process is simulated to carry out targeted virtual enzymolysis, a multi-target molecular docking technology is combined, brand-new bifunctional peptides GAAGDK and DSQSAR are accurately predicted and screened from a specific collagen sequence, and aiming at a dual-action mechanism of AGE-RAGE axis and oxidative stress, the application has the advantages of high sensitivity, high sensitivity and high stability. The composition has remarkable synergistic interaction potential in the aspects of preventing diabetic complications and delaying skin and body senescence, and a brand new core raw material is provided for developing next-generation anti-saccharification functional food and skin care products.

The application relates to a recombinant type I collagen and application thereof, which utilizes the characteristics of Pichia pastoris capable of secreting and expressing recombinant proteins, secretes the recombinant type I collagen on the culture medium supernatant, and reduces the difficulty of protein purification. In addition, the integrin site RGD is inserted into the collagen to form a recombinant type I collagen with high stability and high expression. According to the relative proliferation of cells and the relative adhesion experiment of cells, it can be seen that the recombinant type I collagen has the effects of promoting the proliferation of skin fibroblasts, improving the skin, promoting wound healing, promoting the adhesion of fibroblasts in the skin layer and the like. Therefore, the recombinant type I collagen has great potential applications in the fields of skin care and beauty, medical treatment and the like.

The present disclosure provides a recombinant humanized collagen VIII, which is a triple helix structure, has good cell adhesion activity, and is applied to human body without immune rejection and allergic reaction.

The present invention relates to methods of immunoassay for detecting a biomarker which is an MMP-cleaved fragment of collagen type-I and its use in detecting and / or monitoring diseases such as kidney disease and diseases of the cardiovascular system including the heart.

This invention relates to the fields of molecular cloning and protein expression, and particularly to a Pichia pastoris engineered strain expressing full-length human type III collagen and its applications. The engineered Pichia pastoris strain is obtained by cloning the enzymatically digested human type III collagen gene sequence into an expression vector to obtain a recombinant expression vector, which is then transformed into Pichia pastoris. This engineered Pichia pastoris strain was deposited at the China General Microbiological Culture Collection Center (CGMCC) on November 6, 2024, with accession number CGMCC NO.32510. The Pichia pastoris engineered strain obtained by this invention can efficiently produce the desired human type III collagen through fermentation, exhibiting excellent characteristics such as high yield and absence of endotoxins.

The invention discloses a water-based skin care product containing type IV collagen and a preparation method of the water-based skin care product. The water-based skin care product comprises the recombinant collagen skin care composition and a water-based matrix. The recombinant collagen skin care composition comprises a recombinant IV type collagen, a GLP tripeptide, a PGD tripeptide, a recombinant III type collagen, a recombinant I type collagen and a recombinant human-like collagen according to a specific ratio. According to the invention, the recombinant collagen composition is creatively applied to a water-based skin care product to maintain the collagen of the whole skin layer, so that the effect of comprehensively promoting skin barrier repair is achieved.

Provided is a method for preparing a recombinant human collagen. The method comprises: constructing a genetically engineered bacterium which expresses a prolyl hydroxylase and a recombinant human collagen; culturing the genetically engineered bacterium and performing digestion treatment by means of pepsin to obtain a recombinant human collagen type I or type III. The prolyl hydroxylase comprises P4Ha2, P4Hb and vP4H, and by means of controlling the ratio of the copy numbers of the genes thereof, the recombinant human collagen and a natural human collagen are consistent in hydroxylation modification. Also provided is a large-scale production method for the recombinant human collagen. The prepared recombinant human collagen has completely the same sequence composition and length and substantially the same hydroxyproline content as the natural human collagen, and also has a stable full-length triple helix structure and biological activity without immunogenicity and potential risk of viruses.

The application discloses citrullinated collagen type II polypeptide and application thereof. The amino acid sequence of the polypeptide is Ste-QYMCitADQAAGGLR (SEQ ID NO. 1), wherein the N terminal of glutamine Q in the sequence is connected with octadecanoic acid Ste, and the fourth position in the sequence is modified by citrullination. The citrullinated collagen type II polypeptide has a long drug half-life, can effectively treat rheumatoid arthritis, and has small side effects.

The present application relates to the technical field of biological medicine materials, and particularly relates to a collagen-based repair membrane material for oral cavity and a preparation method thereof. Specifically, the present application comprises the following components: collagen type I, bioactive glass nanoparticles, gamma-aminopropyl triethoxysilane, a crosslinking system, polyaspartic acid, CaCl2, K2HPO4 and Sr-Zn solution. In the present application, polyaspartic acid is used to guide in-situ mineralization of calcium and phosphorus to form an oriented compatible nanocrystalline template, chemical coupling and continuous ion release of silanized bioactive glass are combined to realize time sequence cooperation of mineral deposition and ion supply; a unidirectional freezing structure is used to build a structure gradient with a dense cavity surface and a porous bone side, so as to give the structure the functions of anti-permeation and cell guidance; mild crosslinking containing alcohol is combined to improve the wet strength and control swelling; further, the bone side is activated by Sr-Zn solution to form a directional microenvironment, so as to promote interface mineralization maturity and delay collagen degradation, and realize the oral cavity repair function of multi-module cooperation.

The application discloses a recombinant membrane-targeting type 17 collagen protein and application thereof. The application realizes gene fusion of a COL17A1 key function domain and an independent membrane-targeting structure domain for the first time, constructs a novel "activity-targeting" integrated recombinant membrane-targeting type 17 collagen protein (M-C17a1), effectively integrates function activity and cell targeting property through ingenious structure design, and exhibits excellent performance in resisting skin aging, tissue repair and promoting hair growth. The application also proposes a complete preparation method based on a Pichia pastoris expression system. The expression system has high-density fermentation capacity and secretory expression characteristics, significantly simplifies a purification process, and has relatively low production cost, so that the expression system is suitable for large-scale production of the recombinant protein and has good industrial application prospect.

The invention provides a composition for preventing and improving skin aging and a cosmetic containing the same. The composition comprises a red rice extract and type 21 collagen. The composition disclosed by the invention has a synergistic effect on promoting the generation of type I collagen, so that skin aging can be remarkably prevented and / or improved. Meanwhile, the composition and the product containing the composition play an important role in preventing and improving skin aging and treating and improving skin-related diseases.

This invention belongs to the field of renal pathology testing technology and discloses a method for detecting type IV collagen expression in renal biopsy tissue, comprising the following steps: dewaxing and hydrating paraffin sections of renal biopsy tissue to obtain processed tissue sections; adding the processed tissue sections to a repair solution, heating to boiling, and then cooling to room temperature to obtain repaired tissue sections; adding protease to the repaired tissue sections for enzymatic digestion to obtain enzymatically digested tissue sections; adding a primary antibody to the enzymatically digested tissue sections for preliminary incubation, followed by adding a fluorescein-labeled secondary antibody against the primary antibody for further incubation. This method for detecting type IV collagen expression in renal biopsy tissue is applicable to cases where renal biopsy tissue lacks glomeruli or has too few glomeruli to detect type IV collagen expression. It has high specificity and can provide a new detection method for Alport syndrome in cases where renal biopsy tissue lacks glomeruli.

The invention provides recombinant humanized XXIV type collagen as well as a production method and application thereof, and belongs to the technical field of genetic engineering. The recombinant XXIV type humanized collagen has excellent cell adhesion activity and good biocompatibility, can be used for tissue repair, and does not generate immunological rejection and anaphylactic reaction when applied to a human body.

The application belongs to the technical field of protein engineering, and discloses a humanized collagen type ⅩⅦ polypeptide and a preparation method and application thereof. The amino acid sequence of the humanized collagen type ⅩⅦ polypeptide is shown as SEQ ID NO. 1, and the polynucleotide sequence encoding the humanized collagen type ⅩⅦ polypeptide is shown as SEQ ID NO. 2. The application also provides application of the polypeptide in preparation of a protein product with the functions of preventing hair loss, repairing, anti-wrinkle and skin tightening. The efficacy detection at the cell level proves that the prepared humanized collagen type ⅩⅦ polypeptide can promote the proliferation of hair papilla cells, keratinocytes and fibroblasts, and promote the up-regulation of the expression of the β-Catenin signal pathway protein closely related to hair growth in the hair papilla cells, thereby proving that the humanized collagen type ⅩⅦ polypeptide has the efficacy of preventing hair loss and / or repairing, anti-wrinkle and skin tightening.

The application provides a recombinant type III humanized collagen gel based on macromolecular crowding effect and a preparation method and application thereof. First, an rhCol III solution system is prepared; then a macromolecular crowding bath containing dextran 70, polyethylene glycol 8000 and ascorbic acid is prepared; then the rhCol III solution is added into the macromolecular crowding bath to form rhCol III microgel; finally, a 3D printing molecular exclusion mold system with agarose as a base, containing functional additives and a temperature-sensitive polymer as a temperature-sensitive release and space-restricted component is constructed to realize directional construction of the rhCol III microgel. The application realizes physical gelation of the rhCol in seconds (<15 s) and constructs a physiological microenvironment supporting cell proliferation. The recombinant type III humanized collagen gel prepared by the application can be used for preparing collagen microgel balls, collagen bundles, 3D biological printing and other products, and has a wide range of applications.

The application discloses application of agarwood oil in preparation of a medicine for removing stretch marks. The application finds through experiments that the agarwood oil has the effects of promoting proliferation of fibroblasts, resisting oxidation, promoting generation of collagen type I and promoting generation of elastin, and therefore can be applied to preparation of the medicine for removing stretch marks.

The application discloses application of a recombinant collagen type I-antioxidative peptide composition with an antioxidative function, and belongs to the technical field of biotechnology.The application provides a recombinant collagen type I-antioxidative peptide composition prepared by compounding a recombinant collagen type I and a schisandra antioxidant peptide.The composition has high free radical scavenging activity, and has stability and biological safety;and the antioxidative effect is better than that of single recombinant collagen type I or the schisandra antioxidant peptide.The amino acid sequence of the schisandra antioxidant peptide with antioxidative activity is FYPF, FLPW, FYLPF, ANWLPF, IFSPWL, FPFTYAMMLM, GPSFPIFI or GISWGFL.The application provides a scientific basis and technical support for development of antioxidative products in the fields of medical dressings, functional foods, skin care cosmetics and the like.

The present application relates to the technical field of biological materials, in particular to a double-layer guided tissue regeneration membrane and a preparation method thereof, so as to improve the comprehensive performance of the double-layer guided tissue regeneration membrane. In the preparation, collagen type I is dissolved in acid to prepare a collagen type I solution; then, an alkali is added to adjust the pH value, so as to prepare collagen type I fibers; then, the collagen type I fibers are processed and molded, and gradient freeze-drying is performed, so as to prepare a collagen type I membrane; then, the collagen type I membrane is crosslinked with a crosslinking agent, so as to prepare the double-layer guided tissue regeneration membrane; wherein, the concentration of the collagen type I solution is 0.5-1.5%, and the pH value is 7-9. The double-layer guided tissue regeneration membrane prepared by the above preparation method is composed of a dense layer and a loose layer formed on the surface of the dense layer, the thickness of the dense layer is 0.2-0.8 mm, and the thickness of the loose layer is 0.4-0.8 mm. The tensile strength is greater than or equal to 28 N, the suture strength is greater than or equal to 4.7 N, and the porosity is greater than or equal to 65.8%, which effectively solves the problems of insufficient mechanical performance and low biological safety of the existing double-layer guided tissue regeneration membrane. The preparation process is simple, green and large-scale.

The invention discloses a bionic polypeptide for inducing mineralization in type I collagenous fibers and application of the bionic polypeptide. The amino acid sequence of the novel NCPs bionic polypeptide comprises a collagen binding motif, a calcium ion chelation motif and a cell adhesion sequence; the amino acid sequence of the collagen binding motif comprises sequences as shown in SEQ ID NO. 1 to SEQ ID NO. 3. The novel NCPs bionic polypeptide disclosed by the invention can stabilize an amorphous calcium phosphate precursor and specifically bind to type I collagen, so that ordered intra-fiber mineralization is realized. The polypeptide disclosed by the invention breaks through the limitation of single function of a traditional non-collagen simulant, and provides a core technology for constructing a tissue engineering scaffold of which the structure and the performance are highly bionic to those of natural bones.