24 results about "Collagen i" patented technology

The invention relates to a recombinant I-type humanized collagen with crosslinking property as well as a preparation method and application thereof, and belongs to the technical field of recombinant proteins. The invention relates to recombinant I-type humanized collagen with crosslinking property. The amino acid sequence of the recombinant I-type humanized collagen comprises an amino acid sequence as shown in SEQ ID No.1 or a derivative amino acid sequence with the sequence identity of more than 90% with the sequence as shown in SEQ ID No.1; the recombinant I-type humanized collagen has a triple helix structure. A complete triple helix structure can be efficiently expressed, and the hydrogel has efficient cross-linking capability, so that the hydrogel can be efficiently and stably formed through cross-linking.

A phosphodiesterase type 5 inhibitor is used in the preparation of a medicament for resisting fibrotic diseases. Experiments in animal models of ischemia-reperfusion (UIRI)-induced renal fibrosis, unilateral ureteral obstruction (UUO)-caused kidney fibrosis and idiopathic pulmonary fibrosis show that a PDE5 inhibitor such as tadalafil, sildenafil and vardenafil can significantly inhibit the expression of multiple fibrosis iconic proteins such as fibronectin, collagen I, renal injury molecule-1, and α-skeletal muscle actin in UIRI and UUO renal fibrosis lesions, improves glomerulopathy, degree of renal tubular distension, renal interstitial collagen fiber deposition and inflammatory cell infiltration, reduces the fibrotic area within the lesion, and significantly inhibits the progression of renal fibrosis; and the PDE5 inhibitor can significantly improve smooth muscle proliferation and inflammatory cell infiltration in bronchioles and pulmonary arterioles of idiopathic pulmonary fibrosis lesion, improve damage condition of alveolar tissue, and significantly inhibit the progression of pulmonary fibrosis.

The invention discloses an anti-aging composition as well as a preparation method and application thereof. The composition is prepared from the following components in parts by weight: 10 to 50 parts of ergothioneine; 5 to 25 parts of coenzyme Q10; 50 to 150 parts of 3-SL; 50 to 150 parts of collagen tripeptide; 100 to 500 parts of NAD (nicotinamide adenine dinucleotide); and 50 to 150 parts of glutathione. According to the anti-aging composition disclosed by the invention, various functional components such as the ergothioneine, the coenzyme Q10, the 3SL, the collagen tripeptide, the NAD < + > and the glutathione are reasonably proportioned, so that the anti-aging composition shows a remarkable synergistic anti-aging effect in in-vitro and in-vivo experiments. The anti-aging composition can enhance the cell antioxidant capacity, reduce the active oxygen level and promote the expression of collagen I and SIRT1, and compared with a single component or a non-optimized combination, the anti-aging effect is remarkably improved.

The present invention relates to methods of immunoassay for detecting a biomarker which is an MMP-cleaved fragment of collagen type-I and its use in detecting and / or monitoring diseases such as kidney disease and diseases of the cardiovascular system including the heart.

The invention discloses a method for scar-free healing in treatment of wounds / wounds by miR-185-5p, relates to the field of biological medicine and plastic surgery and beauty repair, and comprises application of scar-free healing in acute / subacute / chronic wounds related to any skin injury (including burn, laceration, bruise, operation, perforation and vaccination) of deep dermis. Wound healing is a series of complex and elaborately-planned overlapping stages arranged according to a time sequence, wherein the stages comprise four stages of hemostasis, inflammation, proliferation and remodeling. No matter at any stage, the skin stability after wound healing and whether scars are formed or not can be directly influenced. The miR-185-5p is used in an animal skin model caused by an acute wound, so that the wound healing time is integrally shortened, the reepithelization formation of the wound is promoted, the number of skin appendages is increased, and the regeneration of hair follicles is promoted. The secretion amount of the collagen I is reduced, and the formation of the collagen III is improved.

The invention discloses an oligopeptide composition, a recombinant polypeptide chain, a recombinant nucleic acid molecule and application. The oligopeptide composition comprises at least one oligopeptide, the amino acid sequence of the oligopeptide is the amino acid sequence of an active domain of HAPLN1 protein, and the oligopeptide composition is used for improving the expression quantity of fibroblasts on the HAPLN1 protein and collagen I.

The invention belongs to the technical field of medical chemistry, and particularly relates to a polycyclic polyketone derivative as well as a preparation method and application thereof in treating hepatic fibrosis. Six polycyclic polyketone compounds are obtained from a fungus M. arundinis HL1 separated from a marine sample in Huilaxian County, Guangdong Province, the series of compounds can significantly inhibit expression of FN, Collagen I, alpha-SMA and other fibrosis-related factors in hepatic stellate cells LX-2, and the compound 2 has a significant inhibition effect at a concentration of 40 [mu] mol / L and has no significant toxicity. And the compound 2 reduces the p-Smad2 / 3 level through dose dependence, does not influence the total Smad2 / 3 level, and plays an anti-fibrosis role by inhibiting a TGF-beta / Smad signal channel. The series of compounds, especially the compound 2, are expected to become anti-hepatic fibrosis candidate drugs, and provide a brand new chemical skeleton for research and development of low-toxicity and high-efficiency anti-hepatic fibrosis drugs.

The invention discloses a new application of benzbromarone or pharmaceutically acceptable salt thereof in preparation of a medicine for treating pulmonary fibrosis. The invention belongs to the technical field of medicine. Experimental results show that the benzbromarone can significantly reduce collagen deposition and inflammatory infiltration in lung tissues, reduce fibrosis area, improve lung functions of patients with pulmonary fibrosis, inhibit lung fibroblast activation and inhibit fibrosis progress. The action mechanism of the gene is related to inhibition of expression of BAG2, FN1, Collagen I and protein. The invention provides a brand new medicine choice for treating pulmonary fibrosis, has the unique advantage of new use of old medicine, and has the potential of short research and development period, known safety and definite curative effect. Meanwhile, the invention further expands the application of benzbromarone in preparation of the protein inhibitor and the in-vitro reagent.

The application belongs to the technical field of medical treatment and discloses a cancer prognosis marker combination and application thereof, which comprises the following markers: CD68, PD-L1, PAN-CK, HLA-DR, CD45, CD11c and Collagen I. The cancer prognosis evaluation model trained by using the 7-marker combination exhibits superior prognosis accuracy compared to a model relying on high-dimensional multi-omics data. The application proposes a multiple immunohistochemistry (mIHC) cancer prognosis evaluation detection product based on the above-mentioned cancer prognosis marker combination. Satisfactory prognosis results are achieved by using only two five-marker combinations. The method exhibits high accuracy in predicting the survival of gastric cancer patients, so that it becomes a low-cost, high-benefit and scalable solution for other cancers.

The application discloses application of extracellular vesicles from Eclipta prostrata L. The extracellular vesicles are extracted from Eclipta prostrata L. by using ultracentrifugation and ultrafiltration, and the extracted vesicles are complete in appearance and good in dispersibility, and have the characteristics of high yield, fast separation, low cost and easy scaling. The extracted vesicles can be successfully taken by animal cells. After being taken, the vesicles play the roles of antioxidation and anti-aging, can significantly reduce ultraviolet-induced matrix metalloproteinase 1 (MMP1) expression, promote collagen I (COL-I) generation, improve ultraviolet-induced skin photoaging, and the effect is stronger than that of Eclipta prostrata L. alcohol extract, so that the extracellular vesicles from Eclipta prostrata L. provide a new strategy for skin aging and anti-aging treatment.

The present invention relates to methods of immunoassay for detecting a biomarker which is an MMP-cleaved fragment of collagen type-I and its use in detecting and / or monitoring diseases such as kidney disease and diseases of the cardiovascular system including the heart.

The application discloses a new application of 3-HPPA in preparing a drug for treating myocardial infarction. By means of modern pharmacological research methods, it is first confirmed that 3-HPPA has a significant myocardial protective effect. Experimental research shows that 3-HPPA can significantly improve the heart function of a myocardial infarction mouse induced by coronary artery ligation, improve the left ventricular ejection fraction and the short axis shortening rate, and reduce the infarction area; meanwhile, 3-HPPA can significantly inhibit the gene expression of a heart failure marker BNP, and down-regulate the expression levels of inflammatory factors IL-6, TNF-alpha and fibrosis related factors TGF-beta and Collagen I. Further mechanism research shows that 3-HPPA also plays an anti-inflammatory, anti-fibrosis and anti-apoptosis role. The application discloses the action characteristics of 3-HPPA in multi-target intervention of the pathological process of myocardial infarction, provides an important candidate compound for development of a new type of anti-myocardial infarction drug, and has a wide clinical application prospect.

The invention relates to the technical field of biological medicines, and mainly relates to application of tenascin-C (Tenascinin-C, TNC) as a prevention and / or treatment target in silicosis pulmonary fibrosis. The technical problem to be solved by the invention is to provide a new target selection for prevention and / or treatment of silicosis and pulmonary fibrosis. According to the technical scheme for solving the technical problem, the invention provides the application of the TNC as a target spot for preventing and / or treating silicosis pulmonary fibrosis. It is found that TNC is highly expressed in silicosis pulmonary fibrosis lung tissue, and expression of extracellular matrix genes and protein of human lung fibroblasts can be increased; the invention further finds that the expression of Collagen I and Fibronectin in the fibroblast can be obviously reduced by knocking down the Tnc in the lung tissue fibroblast, so that the progress of pulmonary fibrosis is inhibited. The invention provides a new target selection for prevention and / or treatment of silicosis pulmonary fibrosis, and has a good application prospect.

The invention belongs to the technical field of polysaccharides, and particularly discloses application of sulfated seaweed polysaccharides in preparation of anti-hepatic fibrosis drugs. According to a structural unit of the seaweed sulfated polysaccharide SPW-05-S2, 1, 4-beta-glucuronic acid and 1, 2-alpha-mannose are alternately connected to form a main chain, mannose, xylose, galactose and fucose form branch chains, the branch chains are located on the C-6 site and the C-3 site of the 1, 2-alpha-mannose, and the branch chains are located on the C6 site and the C-3 site of the 1, 2-alpha-mannose. The branches consist of galactose residues, xylose residues, mannose residues and fucose residues. In-vitro experiments prove that the polysaccharide can be used for remarkably inhibiting the expression of fibrosis related proteins (Fibronectin, Collagen I and alpha-smooth muscle actin), and can be used for remarkably inhibiting the expression of the fibrosis related proteins (Fibronectin, Collagen I and alpha-smooth muscle actin). Meanwhile, the surface plasmon resonance technology finds that the polysaccharide can be combined with PI3K protein. Therefore, the polysaccharide has a potential anti-hepatic fibrosis effect and is expected to become a candidate saccharide drug for treating hepatic fibrosis.

The invention belongs to the technical field of medicines, and particularly relates to application of sophora alcohol O in preparation of anti-hepatic fibrosis medicines. The invention provides the application of the sophora alcohol O in preparing the anti-hepatic fibrosis medicine for the first time, for example, the sophora alcohol O can be applied to preparing the medicine for preventing or treating hepatic cell fibrosis and mouse hepatic tissue fibrosis. The compound also can be used for preparing medicines for inhibiting proliferation or activation of hepatic stellate cells, inhibiting hepatic fibrosis in a living body, inhibiting level rise of fibronectin (FN), inhibiting level rise of alpha-smooth muscle actin (alpha-SMA), inhibiting level rise of type I collagen (collagen I, collagen II, collagen III, collagen III, collagen IV and the like), and inhibiting liver fibrosis in a living body, level rise of fibronectin (FN, FN), level rise of alpha-smooth muscle actin (alpha-SMA) and level rise of alpha-SMA. The invention relates to a medicine for inhibiting the level rise of alanine aminotransferase (ALT) in mouse serum, inhibiting the level rise of aspartate aminotransferase (AST) in mouse serum and inhibiting the level rise of hydroxyproline (HYP) in mouse liver tissue, and the medicine has good patent medicine potential and wide development, transformation and clinical application values and prospects.

The invention discloses an anti-inflammatory anti-aging plant extract composition and a preparation method and application thereof.Plum blossom bud extract and rhodiola rosea extract are compounded, the synergistic inflammatory anti-aging effect is achieved by optimizing the proportion and the extraction technology, the anti-inflammatory activity of the compounded extract is remarkably superior to that of a single extract, and the anti-inflammatory anti-aging plant extract composition has the advantages that the anti-inflammatory anti-aging effect is achieved; expression of key inflammatory factors can be efficiently inhibited, and an'inflammatory aging 'pathway is blocked. Meanwhile, the composition can effectively promote skin fibroblasts to synthesize collagen I, enhance skin elasticity, and synergistically improve aging characterization from multiple dimensions such as repairing skin barriers and the like. And the two core components are derived from natural plants, so that the biocompatibility is good, and the irritation is low. The preparation method provided by the invention is simple and convenient in process, mild in condition, capable of efficiently enriching active ingredients and suitable for large-scale production, an innovative raw material solution is provided for developing efficient and safe anti-inflammatory and anti-aging cosmetics and skin care products, and the application prospect is wide.

The invention discloses a preparation method of Kushui rose fermentation liquor and application of the Kushui rose fermentation liquor in anti-aging cosmetics. According to the preparation method, Kushui rose is taken as a substrate, lactobacillus plantarum TS337821 is taken as zymophyte, and fermentation is carried out under optimal conditions (for example, the inoculation amount is 3%, and fermentation is carried out at 37 DEG C for 45 hours). According to the method, the content of active ingredients such as chlorogenic acid and cyanidin-3-O-glucoside in the fermentation liquor can be remarkably increased. Experiments prove that the obtained fermentation liquor has a definite anti-aging effect, and can effectively improve cell viability, promote synthesis of collagen I, inhibit matrix metalloproteinase-I and enhance activity of antioxidant enzymes in cells. The fermentation liquor can be directly used for preparing anti-aging cosmetics, and the recommended volume fraction in a formula is 0.75%. The invention provides a new technical scheme and a high-quality raw material for high-value development of the Kushui rose.

The invention relates to the field of traditional Chinese medicine, in particular to application of sophora flavescens external vesicles in preparation of a medicine for relieving myocardial fibrosis after myocardial infarction. Experiments discover that the sophora flavescens outer vesicles can significantly improve cardiac super EF and FS values of myocardial infarction mice, improve myocardial infarction areas of myocardial infarction group mice, and inhibit mRNA and protein expression levels of alpha-SMA, collagen I and collagen III, and show that the sophora flavescens outer vesicles can significantly improve cardiac dysfunction and myocardial injury caused by myocardial infarction of mice, and can significantly improve myocardial infarction of mice. Transdifferentiation and matrix collagen deposition of myocardial fibroblasts induced by TGF-beta1 are remarkably relieved, and the compound can be used for preparing drugs for relieving myocardial fibrosis after myocardial infarction and improving cardiac dysfunction and myocardial injury caused by myocardial infarction.

The invention belongs to the technical field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine composition, a preparation, a pharmaceutical composition and application. The traditional Chinese medicine composition is prepared from the following raw materials in mass ratio: 6-12 parts of radix aconiti lateralis preparata, 9-15 parts of cassia twig, 30-50 parts of poria cocos, 15-25 parts of polyporus umbellatus, 15-25 parts of rhodiola rosea and 25-35 parts of herba leonuri. Experimental research shows that the traditional Chinese medicine composition can remarkably reduce the levels of ALT, AST and ALP in Wilson disease model mouse serum and improve the liver function; hepatic tissue collagen deposition is reduced, and fibrosis markers such as HA and Col IV are down-regulated; the expression of alpha-SMA, Collagen I and TGF (Transforming Growth Factor)-beta is inhibited; vEGF (vascular endothelial growth factor) and Ang-1 expression of liver tissues are reduced. The action mechanism relates to multiple targets of improving microcirculation, regulating water metabolism, inhibiting hepatic stellate cell activation and the like.

The invention relates to the technical field of pet joint protection, and discloses an I-type and II-type collagen composite formula for pet joint health and a preparation method of the I-type and II-type collagen composite formula. The type I and type II collagen composite formula for pet joint health comprises the following components: 15 to 25 parts of type I collagen, 15 to 25 parts of type II collagen, 1 to 3 parts of green lip mussel powder, 35 to 45 parts of chicken hydrolysis powder, 5 to 15 parts of fruit and vegetable products and 5 to 10 parts of a carrier. According to the compound collagen pet food, the action disorder caused by joint degeneration or sport injury can be effectively relieved by utilizing a multi-target synergistic mechanism (such as ligament strengthening, cartilage load reduction and inflammation inhibition of the collagen I and the collagen II in pet joints.

A specific polypeptide interfering with the binding of Rac1 and PAK2, a pharmaceutical composition thereof and application, the amino acid sequence of the specific polypeptide is shown as SEQ ID NO:1; the polypeptide blocks the binding of Rac1 and the 81-85 amino acids (EHTIH) of PAK2 protein by competitive binding, inhibits the phosphorylation of PAK2 and the activation of downstream TGF-beta, alpha-SMA and collagen I fibrosis pathway. The application discloses a pharmaceutical composition comprising the polypeptide and application of the polypeptide in preparation of a medicine for treating diabetic kidney fibrosis. In vitro cell experiments show that the polypeptide inhibits the increase of p-PAK2 and the expression of fibrosis factors induced by high glucose in vitro; animal experiments show that the polypeptide can improve the pathological damage of the kidney of diabetic mice, reduce the expression of fibrosis-related factors, and has high specificity and long-acting property, thereby providing a new direction and method for the treatment of diabetic kidney fibrosis.

The invention discloses a preparation method of human umbilical artery stem cell induced vascular organoid, and belongs to the field of biomedical engineering. The method aims to solve the problems of limited cell source, low induction efficiency, incomplete structure function and the like in the existing vascular organ preparation. The method comprises the following steps: separating and culturing human umbilical artery adventitia stem cells, taking the third to fifth generation cells, inducing the third to fifth generation cells for 5 days through an induction culture medium containing VEGF-A, FGF-2, CHIR99021, BMP4, Y27632 and Frosklin, mixing the cell aggregate with Matrigel-Collagen I to construct a three-dimensional system, and culturing the three-dimensional system with a mature culture medium containing VEGF-A and FGF-2 for 10 days to form vascular organs. According to the method, the human umbilical artery adventitia stem cells which are rich in source and free of ethical dispute are adopted, the induction efficiency is remarkably improved through staged induction and optimized factor combination, formed vascular organs are complete in lumen, rich in branch, stable in function, easy and convenient to operate and easy to standardize, and the method can be applied to the fields of cardiovascular disease research, drug screening and regenerative medicine.

The invention discloses application of curculigoside in preparation of a medicine for preventing or treating renal fibrosis, and belongs to the technical field of biological medicine. In an in-vivo experiment, expression levels of Vimentin and alpha-SMA in kidney tissues of UUO model mice can be reduced through intraperitoneal injection of curculigoside, so that cell phenotypic transformation induced by TGF-beta1 is reduced, the collagenous fiber deposition area in Masson dyeing is reduced, the expression level of Collagen I protein in the kidney tissues of the UUO model mice is reduced, and the fibrosis process of the kidney tissues is reduced. Proved by signal generation analysis and cell thermal migration experiments, curculigoside can be combined with EZH2, which prompts that curculigoside can inhibit the fibrosis process through a targeted EZH2 pathway. It is proved for the first time that curculigoside has the effect of relieving renal fibrosis, and a new thought is provided for medicine development of curculigoside in the kidney disease direction.

To provide a method for producing retinal pigment epithelial cells.SOLUTION: A method of producing retinal pigment epithelium (RPE) cells, comprising the steps of: (a) culturing a cell population of undifferentiated human pluripotent stem cells on an adherent surface selected from the group consisting of laminin, fibronectin, vitronectin, collagen I and collagen IV in a medium comprising nicotinamide as a differentiation-inducing agent and lacking activin A under feeder cell-free conditions to obtain differentiated cells; (b) culturing the differentiated cells on the adherent surface in a medium comprising nicotinamide and one or more members of the TGF β superfamily selected from the group consisting of TGF β 1, TGF β 3, and activin A to obtain RPE cells, wherein at least 50% of the cells in the population of cells are Oct4 + TRA-1-60 +.SELECTED DRAWING: Figure 1