94 results about "Collagen i" patented technology

Polymerized type I and/or III collagen based compositions for medical use as adhesives and sealants and preparation thereof are described. Prior to polymerization, the collagen monomers are prepared recombinantly whereby chemical modifications of the collagen are not needed to form such monomers. The type I and/or III collagen compositions are useful as medical adhesives for bonding soft tissues or in a sealant film for a variety of medical uses. In a further aspect of the present invention, the polymerized type I and/or III collagen composition includes agents which induce wound healing or provide for additional beneficial characteristics desired in a tissue adhesive and sealant.

The invention discloses a tissue induction biomedical material, and particularly relates to a degradable active biological membrane/powder. The tissue induction biomedical material is prepared from, by weight, 0.1-99% of degradable medical polyurethane, 0-99.9% of polyhydroxyalkanoate, 0-99.9% of polylactic acid and 1-99.9% of collagen, wherein polyhydroxyalkanoate is one of PHA, PHB, PHBV, PHBHHx and P3HB4HB, polylactic acid is one of polyglycolic acid and polylactic acid-glycolic acid copolymer, and collagen is one or more of collagen I, collagen II, collagen VI, collagen IX, collagen X, collagen XI and other various in-vivo collagen. The ratio of all the components is adjusted according to the requirements of the implanting positions of different in-vivo tissue for material performance, degradation products and PH values, so better clinical service is provided.

The invention relates to a method for constructing a three-dimensional neural stem cell model in two steps by adopting the micro-fluidic technology. The method is characterized in that a rat tail collagen I is used as a three-dimensional support, a micro-pillar array type micro-fluidic chip is used as a culture platform, and a neural stem cell is cultured in two steps, wherein in the early culture stage, a culture medium for promoting the amplification of the neural stem cell is injected into a cell culture chamber, and in the later culture stage, a conditioned medium suitable for the growth of the neural stem cell and the daughter cells thereof is used, and a three-dimensional composite structure which is similar to a nerve tissue is formed by simulating the microenvironment of different neurogenesis stages in the body. The method provided by the invention is good in repeatability and can be used for construction a plurality of groups of samples. The adopted microfluidic culture system is in a microliter volume and can be regulated accurately, thus the amount of various high-cost cell growth factors, immunologic fluorescent antibodies and cell hormones used in the process of culturing the cell can be reduced greatly, and the cell culture cost can be lowered. The three-dimensional neural stem cell model is expected to be a nerve tissue substitute for screening a novel medicament or monitoring an environmental toxin.

The invention relates to a cartilage and bone repair composition comprising a group of human mesenchymal stem cells that are differentiated to the chondro-osteogenic lineage, by means of the amplification thereof with human serum and a transforming growth factor-beta 1 with a molecular domain for binding to collagen I (TGF-β1-DUC) obtained in eukaryotic expression systems, and a biocompatible material which is absorbed by the cells thus treated. The inventive composition can be employed using implants in the area to be repaired or it can be employed directly by injecting the cells in suspension either at the site of the injury or into the systemic circulation for the widespread distribution thereof.

A calcium phosphate bone cement setting to a calcium-deficient hydroxyl apatite is modified by an organic phosphate ester of orthophosphoric acid or a salt of an organic phosphate ester. The base cement contains preferably tricalcium phosphate, dicalcium phosphate (anhydrous), calcium carbonate and precipitated hydroxyl apatite. The organic phosphate ester is added to the base cement in an amount of 0.5 to 5 percent by weight. The bone cement can be modified further by adding mineralized collagen I.

Here the inventors provide a composition for topical application to the skin of animal comprising stearidonic acid in combination with one or octadecatrienoic acids (CODTAs). Preferably the composition comprises echium oil as a source of the stearidonic acid, and a plant lipid as a source of octadecatrienoic acid, such as borage oil (Borago officinalis), wheat germ oil (Triticum vulgare) rosehip oil (refined; Rosa mosqueta), jacaranda oil (Jacandra mimosi folia), and/or calendula oil (Calendula officinalis). The composition of the invention increases collagen I secretion and thus has particular utility for promoting collagen production in skin.

A laminate comprising a transparent collagen I type sheet and a human corneal endothelial cell culture layer provided on the sheet. An endothelial cell culture layer laminate usable in transplantation is provided.

The present disclosure relates to bioactive biomatrix compositions. In one embodiment, the biomatrix composition is derived from human amniotic membrane. This biomatrix is ​​called HuBiogelTM. The HuBiogel™ composition precisely mimics the composition of naturally occurring basement membranes and is capable of supporting a variety of cell types in vitro and in vivo. The disclosed HuBiogel™ biomatrix contains laminin, collagen I, and collagen IV in one embodiment, and may further contain any combination of the following: nestin, tenascin, fibronectin, and proteoglycans . The biomatrix composition is substantially free of endogenous growth factors and proteolytic enzymes. The present invention also describes two-dimensional and three-dimensional culture systems and physiological/pathological model systems using HuBiogel™ compositions. HuBiogel(TM) compositions may be modified to contain desired growth stimulators, such as growth factors, polypeptides and small organic molecules, and may also contain growth inhibitory and/or therapeutic agents.

The invention discloses a preparation method of an in-situ-crossly-linked electrospun fibrous membrane dressing made from collangen I. According to the preparation method, the collagen I is dissolved in a novel environment-friendly solvent system (i.e. a composite system of [EMIM][Ac] and at least one lithium sal), so that the triple-helical structure of the collagen I is reserved to the maximum extent; natural cross-linking agent with good biocompatibility (alginate dialdehyde) is adopted to participate in the electrostatic spinning process of the collagen I, and crossly linked with the collagen I in situ. Secondly, through salt removal by simple water washing, freeze drying, sterilization and packaging, the in-situ-crossly-linked electrospun fibrous membrane dressing is obtained. The preparation method is simple and fast. Through the adoption of the preparation method, industrial production can be realized easily, and potential biotoxicity risks, brought by glutaraldehyde crosslinking agent, which is adopted universally at present, are effectively avoided. The in-situ-crossly-linked electrospun fibrous membrane dressing prepared according to the preparation method has the advantages of high biocompatibility, no cytotoxicity and high biological activity.

The invention discloses a preparation method of a cell preparation for promoting the regeneration and the repair of traumas. The preparation method comprises the steps of shearing and digesting heel tendon tissues, carrying out centrifuging to remove supernate, adding an obtained tendon stem cell population into a culture solution, carrying out resuspension and filtering, inoculating the culture solution into a culture plate for culturing, mixing the culture solution with platelet rich plasma, and adding normal saline, so as to prepare a tendon stem cell population. Tendon stem cells are separated and cultured, tendon stem cells cultured in vitro are mixed with the platelet rich plasma to prepare the cell preparation, the platelet rich plasma contains a large number of growth factors such as PDGF, EGF, TGF-beta1 and IGF which have great promotion effects to the repair of tendons, the platelet rich plasma is capable of promoting the propagation of tendon cells and the expression of collagens I and III in vivo, and the tendon cells and the collagens I and III are key components of the tendons, so that an experimental result has a certain guiding effect to the repair of tendon injury of a human body.

The invention provides a biological root scaffold. The biological root scaffold is prepared from animal-origin collagen I and hydroxyapatite, has certain pore structure in microscopic scale, has porosity 70%-95%, pore diameter 50-500 MuMu and has a macroscopically cylindrical shape, cylinder height is 9-12 mm; the shape of the outer side of a cylinder truncated by an axial plane perpendicular to the cylinder is circular or approximately circular; the scaffold is 11-14 mm in perimeter. The mineralized collagen-based biological root scaffold material and a biological root scaffold can be obtained by implementing the invention. The scaffold material has chemical composition and microscopic structure consistent with dentin of a natural tooth root, the shape of the scaffold is close to that of the natural tooth root, the micrometer porous structure and macroscopic internal structure of the scaffold help carry cells, growth factors, pharmaceuticals and the like for the ingrowth of the cells, and therefore the biological root scaffold has ingredients and structure consistent with those of the natural tooth root, is good for the construction of biological tooth roots in root regeneration application and has a promising clinical application prospect.

The present invention relates to use of hydrogel based on RGD-conjugated alginate with and without addition of nanocellulose and/or fibrin as a novel bioink for 3D Bioprinting of human skin, particularly dermis. RGD-conjugated alginate provides adhesion sites for the human fibroblasts which result in cell adhesion and stretching which contribute to upregulation of genes producing Collagen I. In this invention, RGD-conjugated alginate is used as one of the components of the bioink for 3D bioprinting. Another innovation described herewith is use of coaxial needle when 3D bioprinting with alginate and RGD-modified alginate bioinks. A coaxial needle makes it possible to crosslink the bioink upon 3D bioprinting operation and thus achieve high printing fidelity which is required for high cell viability, proliferation and production of extracellular matrix. In this invention, the novel RGD-modified alginate bioink together with human fibroblasts is 3D bioprinted and the resulting construct shows high cell viability, high cell proliferation, high degree of stretching of fibroblasts and high productivity of Collagen I. The cell bioink construct biofabricated with this invention is ideal for testing cosmetics and active ingredients of skin care products particularly those used for skin regeneration. It is also ideal to be used as skin grafts for skin repair for patients with damaged or burned skin.

Differentiation and stability of neural stem cells can be enhanced by in vitro or in vivo culturing with one or more extracellular matrix (ECM) compositions, such as collagen I, IV, laminin and/or a heparan sulfate proteoglycan. In one aspect of the invention, adult mammalian enteric neuronal progenitor cells can be induced to differentiate on various substrates derived from components or combinations of neural ECM compositions. Collagen I and IV supported neuronal differentiation and extensive glial differentiation individually and in combination. Addition of laminin or heparan sulfate to collagen substrates unexpectedly improved neuronal differentiation, increasing neuron number, branching of neuronal processes, and initiation of neuronal network formation. In another aspect, neuronal subtype differentiation was affected by varying ECM compositions in hydrogels overlaid on intestinal smooth muscle sheets. The matrix compositions of the present invention can be used to tissue engineer transplantable innervated GI smooth muscle constructs to remedy aganglionic disorders.

The invention discloses construction and application for tissue-engineered bone with BMSCs transfected with genes in a combining manner. According to the invention, recombinant lentiviral vectors carrying human bFGF, BMP-2 and GFP genes are constructed, BMSCs are transfected with the genes, the osteogenesis function of BMSCs can be enhanced through human bFGF and BMP2 gene combined-transfection, the fact that bFGF/BMP2 can adjust OPN, collagen-I and other osteogenesis nonspecific gene expression mechanisms is proved, and a GFP-marked tissue-engineered bone is constructed by gene transfected BMSCs composite heterologous bone scaffold material, and a jaw defect animal model is replanted. According to the invention, defects of single gene transfection are overcome, blood vessel forming and osteogenesis functions for solving difficulties in tissue-engineered bone tissue construction and application thereof to jaw defect repairing are obtained, a bone grafting material which has a wide application prospect is provided for mouth rehabilitation and bone transplantation treatment, and a high practical value and practical significance are realized.

In one aspect, the present invention provides methods for determining susceptibility to bone damage in a subject. In some embodiments, the methods comprise screening for polymorphisms in the MTHFR and collagen Iα1 genes that are associated with susceptibility to bone damage. In some embodiments, the methods comprise screening for elevated levels of homocysteine in a subject, wherein elevated levels of homocysteine are associated with an increased risk of bone damage. The methods of the invention may be used in predicting the response of a patient to treatment. Also provided are methods for prevention or reducing the risk of bone damage in a subject.

The present invention provides a cyclic peptide comprising three colinear amino acids, arginine-lysine-lysine (RKK) for binding to integrin α2I domain. Potent inhibitor of laminin-1 interaction. The invention also provides methods of using these peptides to block integrin function and inhibit cell migration.

Methods for deriving and cultivating human embryonic stem (ES) cells and maintaining their pluripotency in culture is provided by utilizing secreted products obtained from the culture medium of human embryonic germ (EG) cell derivatives, such as embryoid body-derived cells. Substrates include compounds such as collagen I, fibronectin, or superfibronectin, or extracellular matrix, typically human derived.