629 results about "Fibrin" patented technology

The invention relates to injectable hydrogel for rapid hemostasis and antibiosis of an oral cavity as well as a preparation method and application of the injectable hydrogel, and the injectable hydrogel comprises the following components in percentage by weight: 5-15% of aminated chondroitin sulfate, 5-15% of oxidized dextran, 1-3% of platelet-rich fibrin and 0.5-1% of quercetin modified zeolite imidazole acid framework nanoparticles. According to the invention, Chs-ADH and ODex are adopted as matrix materials, PRF and ZIF-8 (at) QCT are introduced, the hydrogel is endowed with antibacterial, anti-oxidation and immunoregulation functions, and by optimizing the proportion of the components, the obtained hydrogel has good injectability, stability and degradation resistance, so that the hydrogel can still maintain an effective blood coagulation function in a high-humidity and high-enzymatic-activity oral environment.

The invention relates to the technical field of tumor biomedical engineering and organ chips, in particular to a vascularized gastric cancer organ chip and a preparation method thereof.The chip is composed of an integrated micro-fluidic main body, an annular micro-column array and an optical sealing film, and a central culture cavity is divided into a tumor area and a blood vessel area by micro-columns; the preparation method comprises the following steps: injecting a fibrous protein solution containing human umbilical vein endothelial cells and cancer-related fibroblasts into a vascular region, and adding thrombin for in-situ gelation to form a pre-vascularized network; the method comprises the following steps: mixing a patient-derived gastric cancer organ with a methacrylic acid esterified gelatin pre-polymerized solution, injecting the mixture into a tumor area, and carrying out photo-crosslinking immobilization; culturing for 7-14 days under the dynamic perfusion condition of a mixed culture medium to obtain a three-dimensional gastric cancer organ model containing a capillary network; the method is expected to be used for rapid, low-cost and high-throughput screening of chemotherapy or anti-angiogenesis drugs and accurate prediction of individual curative effects of patients.

The invention relates to the field of environmental protection, and discloses a composite sand solidification agent and a preparation method thereof. The cellulose-chitosan composite material comprises the following components: 40-50 parts of cellulose, 80-100 parts of chitosan, 2-4 parts of a cross-linking agent, 1-2 parts of an initiator, 2-4 parts of a dispersant, 20-30 parts of fly ash, 20-30 parts of silicate clinker, 200-240 parts of a sodium hydroxide solution, 10-15 parts of sodium carbonate, 80-100 parts of an ethanol solution of chloroacetic acid, 250-300 parts of a sodium acetate buffer solution, 80-100 parts of ethanol, 240-320 parts of ethylene glycol and 30-40 parts of water. The cellulose is modified and then copolymerized with the chitosan, then the copolymer is loaded with the fly ash and the silicate clinker hydrate, the prepared composite sand solidification agent has high strength and excellent water absorption and retention capacity and is environmentally friendly, and the raw materials cellulose and chitosan are derived from biological raw materials, are wide in source, are easy to degrade and are free of toxicity and pollution; the fly ash is subjected to roasting pretreatment, and degradation of the sand stabilization material in the later period has no obvious negative effect on the ecological environment.

The invention relates to the technical field of nano materials and biological medicines, in particular to application of structurally reversible gel in preparation of a physical blood coagulation material, the structurally reversible gel comprises loosely assembled nanorod assemblies and a solution for dispersing the loosely assembled nanorod assemblies; the nanorod assembly comprises an inorganic one-dimensional nanorod and anions capable of participating in the assembly of the inorganic one-dimensional nanorod; the inorganic one-dimensional nanorod at least contains rare earth metal elements. The physical blood coagulation material simulates the assembly process of fibrin, shows excellent hemostasis capability independent of natural hemostasis, reduces the blood coagulation factor level, and effectively avoids the thrombus risk caused by excessive blood coagulation. In addition, the material is simple and stable in component, low in preparation cost, high in safety and capable of being stored and used for a long time.

The invention discloses composite gel for osteochondral repair as well as a preparation method and application of the composite gel. Main raw materials of the composite gel are regenerated silk fibroin (RSF) and recombinant humanized III-type collagen (rhCol-III), and auxiliary raw materials of the composite gel are sodium hyaluronate (HA), fibrinogen and thrombin. The preparation method comprises the following steps: firstly, mixing an RSF solution with an rhCol-III solution, obtaining RSF-rhCol particles through a freeze drying technology and mechanical pulverization, dispersing the particles in an HA solution to obtain composite gel, mixing the gel with a thrombin solution, catalyzing fibrinogen to be split and converted into fibrin by thrombin, and performing freeze drying to obtain the rhCol-RhCol-RhCol-RhCol-RhCol-RhCol-RhCol. And crosslinking the fibrous protein monomer to obtain the injectable composite gel preparation capable of immediately gelling. The prepared RSF-rhCol composite gel can adapt to a defect area, has a certain adhesion effect, has excellent biocompatibility, promotes migration of bone marrow mesenchymal stem cells, induces cell differentiation and promotes cartilage tissue regeneration.

The invention belongs to the technical field of biomedical materials, and particularly relates to a photothermal nano-drug containing a guanidyl structure as well as a preparation method and application of the photothermal nano-drug, and the photothermal nano-drug is CA-COFBT-TG (at) TS obtained by taking COFBT-TG containing guanidyl as a carrier, loading a photothermal agent TS and then modifying a fibrin targeting peptide CA on the surface. The photo-thermal nano-drug is a covalent organic framework material platform, can be enriched at a thrombus part, has excellent photo-thermal conversion performance, can effectively remove thrombus at a focus part, and can improve neuronal death after cerebral arterial thrombosis by inhibiting ferroptosis.

The invention belongs to the technical field of biological medicine, and particularly discloses an artificial valve tissue calcification model and a preparation method and application thereof. The preparation method comprises the following steps: providing human valvular interstitial cells; the method comprises the following steps: providing a hydrogel system, wherein the hydrogel system comprises type I collagen, fibrinogen and Matrigel matrigel; constructing an engineered heart valve tissue strip by taking the hydrogel system as a skeleton structure between valve interstitial cells; and culturing the engineered heart valve tissue strip by using a calcification induction culture solution, so that the valve tissue generates a calcification phenotype. The model provided by the invention can generate a valve calcification phenotype which is similar to that after human heart valve calcification and is represented by valve interstitial cell activation, extracellular matrix remodeling such as collagen mass secretion and calcium deposition region generation. The model provided by the invention is low in cost, can be produced in batches, meets the research on the pathogenesis of extracorporeal valve calcification, and makes up for the dilemma that no artificial valve calcification model is available for drug research and development at present.

The invention relates to the field of biological medicine, in particular to a water-soluble alginate microcarrier as well as a preparation method and application thereof. The alginate microcarrier comprises a carrier core structure and a shell surface modifier, the micro-carrier core layer is formed by carrying out plasma crosslinking on water-soluble alginate, ethylenediamine tetraacetic acid salt, nitrilotriacetic acid salt, diethylene triamine pentaacetic acid and water-soluble divalent metal salt, and light-sensitive alginate and a photoinitiator can also be added, so that the micro-carrier core layer has two crosslinking networks; the shell layer is composed of one of gelatin, collagen, fibrinogen, albumin and fibrin, or the gelatin, the collagen, the fibrinogen, the albumin and the fibrin and other biocompatible polymers; the obtained microcarrier has the characteristics of high transparency and rapid degradation, and adherent cells can be amplified in vitro; the microcarrier obtained in the invention can be used for mesenchymal stem cell culture, and can be rapidly degraded under a mild cell condition to harvest cells.

The invention relates to the technical field of high polymer materials, in particular to biological glue applied to fixation of an esophageal hiatus hernia patch. The biological glue comprises 40 to 60 wt% of fibrinogen, 30 to 50 wt% of a polyethylene glycol polymer, 2 to 8 wt% of an amino-containing small molecule compound, 0.2 to 0.8 wt% of a growth factor and 1 to 3 wt% of glutaraldehyde. The biological glue provided by the embodiment of the invention has excellent mechanical properties and good histocompatibility, and can realize a better patch fixing effect in a hiatus hernia operation, so that postoperative recovery of a patient is guaranteed.

The invention discloses a preparation method of a composite spray for improving the hemostatic ability of a wound part, the composite spray comprises a liquid A and a liquid B. The liquid B is a sodium alginate aqueous solution, a preparation method of the solution A comprises the following steps: 1, dissolving a lipopeptide conjugate, cholesterol, distearoyl phosphatidylcholine, distearoyl phosphatidylserine and dimethyl sulfoxide in a chloroform-methanol mixed solution to obtain a mixed system; 2, preparing the mixed system into a precursor solution by utilizing a film hydration method; 3, adding calcium chloride into the precursor solution, and reacting in a dark place to obtain a solution A; a precursor solution is designed according to the function of natural platelets, the function of the precursor solution is equivalent to that of nano platelets, the precursor solution is matched with sodium alginate, adverse effects on a blood coagulation system caused by fibrin degradation, platelet activation capability reduction and the like of a wound part due to blood loss can be relieved in a targeted manner, and the preparation has the advantages of rapid hemostasis, good biological activity, no toxic or side effects and the like. And the wound healing is rapid.

The invention belongs to the technical field of biological medicines, and particularly relates to efficient mineralized hydrogel for bone repair as well as a preparation method and application of the efficient mineralized hydrogel. According to the hydrogel for promoting hard bone repair provided by the invention, hydroxybutyl chitosan (HBC) and deferoxamine (DFO) are introduced into a fibrinogen-thrombin / bioglass system, so that the in-vitro mineralization ability of the material is remarkably improved. HBC provides a good adhesion scaffold for osteoblasts and promotes formation of a mineralized matrix; dFO is chelated with iron ions, a hypoxia environment is simulated, expression of osteogenesis related genes is activated, and deposition of mineralized matrixes is further promoted. And more and denser inorganic mineral substances are deposited on the surface of the material through the synergistic effect of the two.

The invention relates to the technical field of hydrogel, in particular to composite hydrogel capable of stopping bleeding, resisting bacteria and promoting alveolar bone repair, a preparation method and application. The composition comprises a polyacrylic acid solution, a calcium chloride solution, a disodium hydrogen phosphate solution, platelet-rich plasma (including plasma, platelet lysis buffer, platelet-rich fibrin and fibrin of high-concentration concentrated growth factors) and nano clay (hectorite). The preparation method has the advantages of simple process, easily available raw materials, environment-friendly production process, safety and short preparation period. The composite hydrogel is good in biocompatibility and injectable, and can be widely applied to the field of biomedical materials.

The invention discloses inonotus obliquus polysaccharide IOP-1 capable of resisting non-small cell lung cancer activity and application, and belongs to the field of biological medicine. The method for extracting the inonotus obliquus polysaccharide by boiling water and precipitating the inonotus obliquus polysaccharide by ethanol is combined with a DEAE (diethylaminoethanol) cellulose column purification technology, and the principle that macromolecular polysaccharide is insoluble in an organic solvent is utilized, so that the separation of the inonotus obliquus polysaccharide is successfully realized, and the inonotus obliquus polysaccharide IOP-1 with non-small cell lung cancer resisting activity is obtained. The monosaccharide of the inonotus obliquus polysaccharide IOP-1 comprises fucose, rhamnose, arabinose, galactose, glucose, xylose and mannose, wherein the molar mass ratio of the fucose to the rhamnose to the arabinose to the galactose to the glucose to the xylose to the mannose is 2.41: 0.58: 1.03: 2.17: 82.20: 4.33: 7.29. In-vivo and in-vitro experiments prove that the traditional Chinese medicine composition has a remarkable effect of resisting the non-small cell lung cancer, and a novel treatment method is provided for clinical treatment of the non-small cell lung cancer.

The invention belongs to the technical field of biological medicine, and particularly relates to application of corynebacterium casei in preparation of medicine for preventing and treating lung injury. By constructing a radon inhalation-induced lung injury mouse model, after exposure at different radon levels, the weight of a mouse is reduced, the lung coefficient is increased, the tidal volume of the lung, the ventilation volume per minute and the maximum inspiration flow are reduced, the respiratory function is inhibited, the alveolar cavity of the mouse is abnormally expanded, the pulmonary septum is thickened and fractured, and more fibrin exudation is accompanied; the traditional Chinese medicine composition can be used for effectively inhibiting pulmonary fibrosis and lung injury due to obvious inflammatory infiltration beside bronchial tubes, extravasated blood in small blood vessels and lung tissue collagen deposition, and can be used for gavage of corynebacterium casei to effectively inhibit the above phenomena. Therefore, the corynebacterium casei can be used for preventing and treating lung injury, especially lung injury caused by radon exposure.

A majority of military casualties occur during the pre-hospital period. The cause of death is largely associated with massive traumatic bleeding that leads to organ damage due to sustained hypoxia. Currently, therapeutics are lacking at the point of injury to mitigate hypoxic damage and maintain the survivability of severe hemorrhage prior to reaching medical facilities. This invention addresses that by introducing a method of co-administering prolyl hydroxylase domain inhibitor (PHDi, MK-8617), anti-fibrinolytic agent (tranexamic acid), and bradykinin receptor antagonist (icatibant) as anti-hemorrhage agents in combination with resuscitation fluid treatment with colloid solution of 25% human albumin that has an advantage of relatively small volume requirement to maintain blood volume. In addition, a kit comprising anti-hemorrhage agents that can be easily carried to the battlefield is also provided here. The therapeutic application of those agents on or near the point of injury can stabilize hypoxia inducible factor-1 alpha and enhance blood clot formation, which improves the patient's cellular adaptation to hypoxia and reduce hemorrhage, and thus can decrease organ failure and increase the patient's survivability.

The invention provides application of enhanced mesenchymal stem cells and exosomes in chronic nephritis and renal failure, and belongs to the technical field of biological medicine manufacturing. Through composite pretreatment of salidroside and astragalus polysaccharide, the treatment potential of exosomes secreted by the mesenchymal stem cells is remarkably enhanced, a new thought is provided for intervention of the chronic kidney diseases, and various pathologies of the chronic kidney diseases are covered. In addition, a 3D microenvironment constructed by fibrin gel is combined to simulate an in-vivo extracellular matrix, the natural growth state of the stem cells is better fitted, directional differentiation and function regulation of the stem cells are promoted, and therefore the biological activity and targeting performance of the exosome are improved.

The invention is applicable to the technical field of cell engineering, and provides a mitochondria-enhanced dental pulp mesenchymal stem cell and a preparation method and application thereof, and the preparation method comprises the following steps: co-culturing platelet-rich fibrin and dental pulp mesenchymal stem cells by using a Transwell chamber to obtain the mitochondria-enhanced dental pulp mesenchymal stem cell. The platelet-rich fibrin transmits mitochondria to the dental pulp mesenchymal stem cells to improve the regeneration capacity of the dental pulp mesenchymal stem cells. According to the invention, an autologous platelet enrichment, namely platelet-rich fibrin, is used as a mitochondria donor with a respiratory function, mitochondria is continuously transferred to the dental pulp-derived mesenchymal stem cells through a cell bioengineering technology, and the mitochondria-enhanced dental pulp mesenchymal stem cells are constructed and applied to tissue damage repair; the vascular regeneration regulation and control capability of the dental pulp mesenchymal stem cells is obviously improved, and the existing mesenchymal stem cell treatment technology is obviously improved.

The growth factor profile, connective tissue matrix constituents, and immunoprivileged status of urodele extracellular matrix (ECM) and accompanying cutaneous tissue, plus the presence of antimicrobial peptides there, render urodele-derived tissue an ideal source for biological scaffolds for xenotransplantation. In particular, a biological scaffold biomaterial can be obtained by a process that entails (A) obtaining a tissue sample from a urodele, where the tissue comprises ECM, inclusive of the basement membrane, and (B) subjecting the tissue sample to a decellularization process that maintains the structural and functional integrity of the extracellular matrix, by virtue of retaining its fibrous and on-fibrous proteins, glycoaminoglycans (GAGs) and proteoglycans, while removing sufficient cellular components of the sample to reduce or eliminate antigenicity and immunogenicity for xenograft purposes. The resultant urodele-derived biomaterial can be used to enhance restoration of skin homeostasis, to reduce the severity, durations and associated damage caused by post-surgical inflammation, and to promote progression of natural healing and regeneration processes. In addition, the biomaterial promotes the formation of remodeled tissue that is comparable in quality, function, and compliance to undamaged human tissue.

The invention discloses injectable hydrogel for cartilage injury repair and a preparation method of the injectable hydrogel. The gel is prepared by crosslinking 3 to 4 parts of recombinant III type humanized collagen-sodium hyaluronate gel particles, 0.5 to 1 part of thrombin solution and 4 to 6 parts of fibrinogen solution. The recombinant III type humanized collagen-sodium hyaluronate-thrombin-fibrinogen gel for injection, prepared by the preparation method disclosed by the invention, has the advantages of high elastic modulus, good biocompatibility, good needle passing performance and long in-vivo degradation period. Particularly, the recombinant III type humanized collagen-sodium hyaluronate-thrombin-fibrinogen gel for injection prepared by the invention can effectively promote regeneration of cartilage tissues, and is an innovative cartilage repair gel.

The invention discloses a plasmin lumbrukinase peptide dietary therapy formula suitable for rehabilitation of cardiovascular and cerebrovascular diseases, blood circulation promoting, blood stasis removing, thrombolysis, collateral dredging and microcirculation improving, and relates to the technical field of medicine and food homology dietary therapy. Soybean protein isolate and earthworm protein are used as core thrombolytic active raw materials, isomaltooligosacharide, xylitol, German Jilida collagen peptide, soybean peptide powder, Belgian imported inulin, red yeast rice and natto powder are compounded, microcrystalline cellulose and magnesium stearate are used as auxiliary materials, and probiotics / metabiotics strains such as rhamnolactobacilli JYLR-005 and the like can be selectively added. The raw materials have a synergistic effect through precise matching, plasmin and lumbrukinase peptide in earthworm protein can directly dissolve thrombus fibrin, natto powder and red yeast rice assist in lowering lipid and dissolving thrombus, multiple active peptides and prebiotics regulate intestinal microecology to improve microcirculation of the whole body, probiotics / metabiotics further strengthen the metabolic capacity of the body, no chemical medicine is added in the whole process, and the traditional Chinese medicine composition is free of toxic and side effects and free of toxic and side effects. The safety is high; the dietary therapy composition can achieve postoperative rehabilitation conditioning of cardiovascular and cerebrovascular diseases through daily consumption, effectively promote blood circulation to remove blood stasis, dissolve thrombus and dredge collaterals, improve microcirculation and relieve various discomfort caused by blood vessel clogging, raw materials are easy to obtain, compatibility is scientific, and the dietary therapy composition is suitable for large-scale production and popularization.

The invention belongs to the field of bioengineering, and discloses construction of anti-micro-nano bubble escherichia coli and application of the anti-micro-nano bubble escherichia coli in efficient carbon sequestration synthesis of succinic acid. Escherichia coli for producing succinic acid is used as a starting strain, amyloid fibrin (Curli) is amplified and constructed to form an overexpression plasmid of a key gene CsgA, and the starting strain is introduced to obtain an overexpression strain. According to the constructed micro-nano bubble resisting escherichia coli, CO2 micro-nano bubbles serve as the only CO2 donor, no carbonate or bicarbonate needs to be supplemented in the anaerobic fermentation process, cells have excellent resistance to the CO2 micro-nano bubbles, growth and metabolism of the cells are not remarkably inhibited, good succinic acid production performance is kept, and the method is suitable for industrial production. The introduction of CsgA greatly improves the actual utilization efficiency of CO2 gas by thalli, is beneficial to reducing the production cost of succinic acid and improving the environmental benefits of succinic acid, and has wide social and economic values.

In a composition, at least one of TQ and NADH is loaded in a gel. The composition disclosed by the invention is loaded on a gel carrier formed by mixing fibrinogen and thrombin, the gel carrier is smeared on a wound, and then the gel carrier, red light and blue light have a synergistic effect, so that the dual functions of resisting infection and accelerating wound healing are realized under the condition that the illumination time is remarkably shortened. In this way, ramp is used; the combination of bLED and the fibrin gel provides an effective scheme for managing chronic wounds, and is expected to be used for clinical intervention of family chronic diabetic wounds.

An oral care composition with retinoid compound, amino acid, and / or olive oil. An oral care composition with retinoid compound, a pentapeptide, such as palmitoyl KTTKS [SEQ ID NO. 2], and / or an olive oil. Methods of preventing gingival recession, stopping gingival recession, reversing gingival recession, decrease gum recession, increase gingival barrier protection, promoting collagen synthesis, promoting extracellular matrix synthesis, increasing gum resilience, increasing epidermal thickness and / or promoting fibrillin synthesis, in an oral cavity of an animal by contacting the oral care compositions with at least one surface of the oral cavity.

The present application provides a vaccine comprising: 1) a peptide oligomer consisting of 2-8 copies of a bacteriophage T4 fibrin C-terminal polypeptide and one or more antigen proteins; or 2) a coding nucleic acid of item 1) wherein 2-8 copies of the bacteriophage T4 fibrin C-terminal polypeptides in the peptide oligomer are directly linked in series through chemical bonds or each of the bacteriophage T4 fibrin C-terminal polypeptides is linked to each other through a peptide linker, and the peptide oligomer and the antigen protein are directly connected through a chemical bond or connected through a peptide linker. In the bi-T4-virus antigen vaccine platform provided by the invention, the antigen can be subjected to multi-component change, that is, the antigen can form a trimer, so that the use flexibility of the vaccine platform is enhanced, the application range of the vaccine platform is widened, and the bi-T4 structure can enhance the formation of trimer protein and enhance the immune aging.

The invention provides autologous platelet-containing plasma gel and a preparation method and application thereof, and relates to the technical field of biological medicine and tissue engineering.The preparation method comprises the following steps that peripheral venous blood of a patient is extracted and added into a centrifugal tube mixed with a sodium citrate anticoagulant; putting the centrifugal tube into a centrifugal machine for centrifuging, and taking upper-layer plasma; wherein the upper-layer plasma refers to plasma between the upper-layer liquid level and the lower-layer red blood cell layer by 1mm; the centrifugal speed ranges from 1300 r / min to 1700 r / min; the centrifugation time is 3-5 min; under a sterile condition, preparing a calcium gluconate / thrombin mixed solution; and dropping the calcium gluconate / thrombin mixed solution into the upper plasma, mixing, and solidifying to obtain the autologous platelet-containing plasma gel. After one-time centrifugation, upper-layer full-layer plasma is extracted, the volume of the prepared gel is far larger than that of a traditional method, the utilization rate of whole blood platelets and fibrinogen is close to 100%, and regeneration and repair of large-area tissue wounds can be met.

The invention provides a wound hemostasis device for the emergency department, and relates to the technical field of wound hemostasis. The wound hemostasis device for the emergency department comprises an inflatable hemostasis air bag, and a constant-temperature film bag is fixedly connected to the lower surface of the inflatable hemostasis air bag. During working, a second infusion pump is started and pumps water in a constant-temperature water storage tank through a second infusion pipeline, the water enters a constant-temperature film bag through a fourth infusion pipeline, the temperature of a wound is guaranteed, meanwhile, a first infusion pump is started, a first water valve on a first infusion pipeline is opened, and a second water valve on a second infusion pipeline is opened. Water in the constant-temperature film bag is pumped through the first liquid pumping pipeline, and finally the water enters the constant-temperature water storage tank, so that the water in the constant-temperature film bag is always in a constant-temperature state, the temperature is ensured to be close to the optimum point of thrombin reaction, platelet aggregation and fibrin formation can be accelerated, and the method is safe and efficient, can minimize tissue damage, and is suitable for clinical application. And the hemostasis speed is accelerated.

The invention discloses a medicinal preparation for promoting healing of chronic diabetic wounds as well as a preparation method and application of the medicinal preparation, and relates to the technical field of medicinal preparations. According to the invention, a thrombin solution containing Se-CQDs and a fibrinogen solution containing GOX are mixed and reacted to prepare the pharmaceutical preparation for promoting healing of chronic wounds of diabetes. The pharmaceutical preparation for promoting healing of the chronic wound of diabetes can effectively remove excessive ROS at the chronic wound of diabetes and reduce the pH, glucose content and M1 / M2 macrophage proportion at the chronic wound, so that the high-glucose alkaline inflammation microenvironment of the chronic wound of diabetes is reversed, collagen deposition, revascularization and skin tissue reepithelization are promoted, and the healing of the chronic wound of diabetes is promoted. Therefore, healing of chronic diabetic wounds is promoted. Meanwhile, Se-CQDs and GOX are combined to prepare the pharmaceutical preparation, and the pharmaceutical preparation has a synergistic effect on promoting healing of chronic wounds of diabetes mellitus.

The invention relates to the technical field of thrombus mining robots, in particular to a micron robot with an asymmetric cilia structure, a preparation method and application. The micro-robot is of an asymmetric composite structure and is composed of a smooth hemisphere and a cilia-shaped structure, sandwich type layered arrangement of a PLGE cilia-shaped nano structure, an MNPs interlayer and a PS sphere core is formed on the surface and the interior of the micro-robot from outside to inside, and cooperative regulation and control of morphology and functions are achieved by regulating and controlling the material proportion of PS and PLGE. The thrombus mining robot shows an efficient thrombolysis effect in a plurality of in-vitro and in-vivo models, and rapid fibrinolysis, deep thrombus penetration and efficient mining of single red blood cells are realized, so that the limitation of a traditional thrombolysis agent is overcome. In addition, the thrombus digging robot has excellent biocompatibility and biological safety, has no obvious cytotoxicity or damage to main organs, and supports the safety of the thrombus digging robot in clinical application.

The invention discloses combined probiotics for improving sarcopenia as well as a preparation method and application of the combined probiotics, and belongs to the field of probiotics. The invention relates to a combined probiotic for improving sarcopenia. The combined probiotic comprises a lactobacillus reuteri body, a lactobacillus johnsonii body, a lactobacillus fermentum body and a bifidobacterium adolescentis body. The combined probiotics disclosed by the invention can be used for remarkably improving the overall symptoms of sarcopenia model mice, including inhibiting weight loss, constipation symptoms and colonic mucosa destruction, prolonging load swimming time, improving muscle endurance, improving skeletal muscle atrophy, improving the expression of I-type slow muscle fiber protein MyHC I and promoting slow muscle fiber repair, and has the effects of preventing and treating sarcopenia. The invention discloses a probiotic intervention method for treating sarcopenia, which comprises the following steps of: preparing probiotics for treating sarcopenia, reducing the content of inflammatory cytokines in muscular tissues, increasing the content of hepatic glycogen, improving systematic metabolism of mice with sarcopenia and damaged serum markers, including lactic acid, lactic dehydrogenase, creatine kinase, creatinine, alanine transaminase ALT and aspartate transaminase AST, providing a new scheme for probiotic intervention for sarcopenia, and being used for preparing related functional products or intervention means.

The invention discloses biological glue and a preparation method thereof.The preparation method comprises the steps that gel and activation liquid are prepared, and the gel is prepared by evenly mixing I-type collagen freeze-dried powder, chitosan and a fibrinogen solution; and mixing the freeze-dried thrombin with a calcium chloride solution to prepare an activation solution. The meninx suturing device has the advantages that sliding in the meninx suturing process is effectively reduced, an operation is easier to carry out, the risk that the meninx or a repairing material slides after the operation can be reduced, and a wound is evenly healed; most of the components are type I collagen which is beneficial to meninx injury repair, and the components contain chitosan which has antibacterial and hemostatic effects.