215 results about "Fibrinogen" patented technology

The invention discloses composite gel for osteochondral repair as well as a preparation method and application of the composite gel. Main raw materials of the composite gel are regenerated silk fibroin (RSF) and recombinant humanized III-type collagen (rhCol-III), and auxiliary raw materials of the composite gel are sodium hyaluronate (HA), fibrinogen and thrombin. The preparation method comprises the following steps: firstly, mixing an RSF solution with an rhCol-III solution, obtaining RSF-rhCol particles through a freeze drying technology and mechanical pulverization, dispersing the particles in an HA solution to obtain composite gel, mixing the gel with a thrombin solution, catalyzing fibrinogen to be split and converted into fibrin by thrombin, and performing freeze drying to obtain the rhCol-RhCol-RhCol-RhCol-RhCol-RhCol-RhCol. And crosslinking the fibrous protein monomer to obtain the injectable composite gel preparation capable of immediately gelling. The prepared RSF-rhCol composite gel can adapt to a defect area, has a certain adhesion effect, has excellent biocompatibility, promotes migration of bone marrow mesenchymal stem cells, induces cell differentiation and promotes cartilage tissue regeneration.

The invention belongs to the technical field of biological medicine, and particularly discloses an artificial valve tissue calcification model and a preparation method and application thereof. The preparation method comprises the following steps: providing human valvular interstitial cells; the method comprises the following steps: providing a hydrogel system, wherein the hydrogel system comprises type I collagen, fibrinogen and Matrigel matrigel; constructing an engineered heart valve tissue strip by taking the hydrogel system as a skeleton structure between valve interstitial cells; and culturing the engineered heart valve tissue strip by using a calcification induction culture solution, so that the valve tissue generates a calcification phenotype. The model provided by the invention can generate a valve calcification phenotype which is similar to that after human heart valve calcification and is represented by valve interstitial cell activation, extracellular matrix remodeling such as collagen mass secretion and calcium deposition region generation. The model provided by the invention is low in cost, can be produced in batches, meets the research on the pathogenesis of extracorporeal valve calcification, and makes up for the dilemma that no artificial valve calcification model is available for drug research and development at present.

The invention relates to the technical field of biological medicine, in particular to a targeting nanoparticle and a preparation method and application thereof. The targeted nano-particle designed and synthesized by the invention comprises a nano-liposome and low-molecular heparin entrapped in the nano-liposome, wherein the surface of the nano-liposome is modified with CREKA peptide. Wherein the CREKA peptide can target fibrinogen in thrombus and specifically recognize FN1 at the thrombus part, so that LMWH is delivered to the thrombus part in a targeted manner, the local antithrombotic effect is improved, meanwhile, the risks of extra bleeding and toxicity are reduced to the maximum extent, and the limitation of traditional LMWH is solved.

The invention relates to the field of biological medicine, in particular to a water-soluble alginate microcarrier as well as a preparation method and application thereof. The alginate microcarrier comprises a carrier core structure and a shell surface modifier, the micro-carrier core layer is formed by carrying out plasma crosslinking on water-soluble alginate, ethylenediamine tetraacetic acid salt, nitrilotriacetic acid salt, diethylene triamine pentaacetic acid and water-soluble divalent metal salt, and light-sensitive alginate and a photoinitiator can also be added, so that the micro-carrier core layer has two crosslinking networks; the shell layer is composed of one of gelatin, collagen, fibrinogen, albumin and fibrin, or the gelatin, the collagen, the fibrinogen, the albumin and the fibrin and other biocompatible polymers; the obtained microcarrier has the characteristics of high transparency and rapid degradation, and adherent cells can be amplified in vitro; the microcarrier obtained in the invention can be used for mesenchymal stem cell culture, and can be rapidly degraded under a mild cell condition to harvest cells.

The invention relates to the technical field of high polymer materials, in particular to biological glue applied to fixation of an esophageal hiatus hernia patch. The biological glue comprises 40 to 60 wt% of fibrinogen, 30 to 50 wt% of a polyethylene glycol polymer, 2 to 8 wt% of an amino-containing small molecule compound, 0.2 to 0.8 wt% of a growth factor and 1 to 3 wt% of glutaraldehyde. The biological glue provided by the embodiment of the invention has excellent mechanical properties and good histocompatibility, and can realize a better patch fixing effect in a hiatus hernia operation, so that postoperative recovery of a patient is guaranteed.

The invention discloses a preparation method and application of autologous fibrin glue, and belongs to the field of biomedical materials. The preparation method comprises the following five steps: collecting autologous venous blood, adding a sodium citrate anticoagulant with the volume ratio of 1: (8-12), and storing at 4-6 DEG C for not more than 2 hours; performing step-by-step centrifugation at the speed of 1500 to 2000 r / min and the speed of 3500 to 4000 r / min to obtain a fibrinogen concentrate; the thrombin-calcium chloride activation solution A and the tranexamic acid activation solution B are mixed according to the ratio of 1: (2-3) to form a composite activation system; mixing the two components according to a mass ratio of 1: (0.8-1.2), and reacting at 25-37 DEG C for 3-5 minutes to form colloid; and pre-freezing at-40 to-60 DEG C, performing vacuum freeze-drying, and storing at 4-8 DEG C. During application, the colloid can be coated on an operation wound surface after being redissolved, or mixed with a growth factor to prepare a dressing for covering a chronic wound surface. The colloid is high in purity, stable in coagulation time, long in storage period, suitable for scenes such as surgical hemostasis and the like, and high in clinical value.

The invention discloses injectable hydrogel for cartilage injury repair and a preparation method of the injectable hydrogel. The gel is prepared by crosslinking 3 to 4 parts of recombinant III type humanized collagen-sodium hyaluronate gel particles, 0.5 to 1 part of thrombin solution and 4 to 6 parts of fibrinogen solution. The recombinant III type humanized collagen-sodium hyaluronate-thrombin-fibrinogen gel for injection, prepared by the preparation method disclosed by the invention, has the advantages of high elastic modulus, good biocompatibility, good needle passing performance and long in-vivo degradation period. Particularly, the recombinant III type humanized collagen-sodium hyaluronate-thrombin-fibrinogen gel for injection prepared by the invention can effectively promote regeneration of cartilage tissues, and is an innovative cartilage repair gel.

The invention provides autologous platelet-containing plasma gel and a preparation method and application thereof, and relates to the technical field of biological medicine and tissue engineering.The preparation method comprises the following steps that peripheral venous blood of a patient is extracted and added into a centrifugal tube mixed with a sodium citrate anticoagulant; putting the centrifugal tube into a centrifugal machine for centrifuging, and taking upper-layer plasma; wherein the upper-layer plasma refers to plasma between the upper-layer liquid level and the lower-layer red blood cell layer by 1mm; the centrifugal speed ranges from 1300 r / min to 1700 r / min; the centrifugation time is 3-5 min; under a sterile condition, preparing a calcium gluconate / thrombin mixed solution; and dropping the calcium gluconate / thrombin mixed solution into the upper plasma, mixing, and solidifying to obtain the autologous platelet-containing plasma gel. After one-time centrifugation, upper-layer full-layer plasma is extracted, the volume of the prepared gel is far larger than that of a traditional method, the utilization rate of whole blood platelets and fibrinogen is close to 100%, and regeneration and repair of large-area tissue wounds can be met.

The invention discloses a medicinal preparation for promoting healing of chronic diabetic wounds as well as a preparation method and application of the medicinal preparation, and relates to the technical field of medicinal preparations. According to the invention, a thrombin solution containing Se-CQDs and a fibrinogen solution containing GOX are mixed and reacted to prepare the pharmaceutical preparation for promoting healing of chronic wounds of diabetes. The pharmaceutical preparation for promoting healing of the chronic wound of diabetes can effectively remove excessive ROS at the chronic wound of diabetes and reduce the pH, glucose content and M1 / M2 macrophage proportion at the chronic wound, so that the high-glucose alkaline inflammation microenvironment of the chronic wound of diabetes is reversed, collagen deposition, revascularization and skin tissue reepithelization are promoted, and the healing of the chronic wound of diabetes is promoted. Therefore, healing of chronic diabetic wounds is promoted. Meanwhile, Se-CQDs and GOX are combined to prepare the pharmaceutical preparation, and the pharmaceutical preparation has a synergistic effect on promoting healing of chronic wounds of diabetes mellitus.

The invention discloses biological glue and a preparation method thereof.The preparation method comprises the steps that gel and activation liquid are prepared, and the gel is prepared by evenly mixing I-type collagen freeze-dried powder, chitosan and a fibrinogen solution; and mixing the freeze-dried thrombin with a calcium chloride solution to prepare an activation solution. The meninx suturing device has the advantages that sliding in the meninx suturing process is effectively reduced, an operation is easier to carry out, the risk that the meninx or a repairing material slides after the operation can be reduced, and a wound is evenly healed; most of the components are type I collagen which is beneficial to meninx injury repair, and the components contain chitosan which has antibacterial and hemostatic effects.

Disclosed is a method for filtering a fibrinogen composition, including the following steps: a) purifying the fibrinogen composition by chromatographic purification using an elution buffer comprising arginine; b) optionally, at least one step of filtering the fibrinogen composition obtained by chromatographic elution in step a), on a filter having a pore size of between 0.08 μm and 0.22 μm, c) filtering the fibrinogen composition obtained by chromatographic elution in step a), or optionally obtained in step b), on a symmetrical filter having a pore size of between 15 nm and 25 nm, and preferably between 18 nm and 22 nm, and d) recovering the resulting fibrinogen solution, the filtering method being carried out without adding arginine after step a), at a high capacity and without a prior freezing and / or thawing step.

A system for determining a functional fibrinogen concentration from a patient's coagulating blood or blood plasma sample is configured to obtain a signal representing physical or biochemical properties of the developing fibrin network during coagulation of the patient's blood or blood plasma sample over time, determine the fibrinogen concentration based on one or more values of one or more features of the signal with the help of a learned function which maps values of the feature(s) to functional fibrinogen concentrations, and output the fibrinogen concentration, e.g. on a display. The feature(s) include(s) multiple of features related to 1) a maximum value of the gradient of the signal, 2) the maximum of the second derivative of the signal, 3) a difference between the maximum and the minimum of the signal, and 4) the minimum of the second derivative of the signal, or at least feature 1) or 2).

The invention discloses a preparation method of a fibrous protein / bacterial cellulose efficient hemostatic patch. The preparation method comprises the following steps: (1) in-situ preparation of large-aperture bacterial cellulose; (2) infiltration of fibrinogen in the large-aperture bacterial cellulose; and (3) enzyme digestion and freeze-drying treatment of fibrinogen. The problems that fibrinogen is fast in degradation and poor in stability and mechanical property are solved, the prepared fibrous protein / bacterial cellulose efficient hemostatic patch can prevent secondary bleeding and avoid thrombus to a certain extent, the preparation process is simple and easy to implement, and large-scale preparation and storage can be achieved.

The present invention is applicable to the technical field of bone defect repair, and provides a preparation method and application of a collateral-modified Gelma hydrogel. The present invention performs collateral modification on the Gelma hydrogel by Fibrinogen, successfully improving the mechanical properties and cell adhesion of the hydrogel scaffold. At the same time, the loading of NELL‑1 protein significantly improves the scaffold's ability to repair bone defects. The Fibrinogen‑Gelma / NELL‑1 hydrogel scaffold constructed by the present invention has shown ideal mechanical properties in relevant characterizations, and has been shown to play a key role in the repair of bone tissue defects in in vitro and in vivo experiments, and has greatly promoted the expression of osteogenesis-related genes. Therefore, the present invention is expected to be applied to clinical treatments related to bone defect repair, such as maxillary sinus bone augmentation and guided bone regeneration (GBR), in the future.

Provided are a sheet-like tissue-reinforcing material and a method for producing the same, the sheet-like tissue-reinforcing material being described below in order to achieve a good tissue-reinforcing effect and workability without requiring the use of fibrinogen and blood clotting ability from a suitable patient. This sheet-like tissue adhesion reinforcing material is provided with: a base sheet having biodegradability and a porous communicating structure; and an adhesive resin layer fixed by ethanol having a water content of 1% or less or acetone having a water content of 1-3% and formed thereon. The adhesive resin layer includes: a first reactant containing an aldehyde dextran; and a second reactant containing a partially carboxylated polylysine. The adhesive resin layer has: a granular structure of a powder derived from the first reactant; and a connection layer which is derived from the second reactant, connects the granular structures to each other, and fixes the granular structures to the base sheet. In order to produce a sheet-like structure reinforcing material, a mixed powder of a two-reactant-type adhesive comprising a powder of a first reactant and a powder of a second reactant is used. For example, the mixed powder is dispersed in ethanol having a water content of less than 1%, and the dispersion is applied to a base sheet and dried.

The utility model discloses a hemostasis assist device for vascular surgery, which relates to the technical field of medical instruments, and comprises a fixing mechanism, a vascular surgery hemostasis mechanism is arranged in the fixing mechanism, the vascular surgery hemostasis mechanism comprises a knob, and the bottom of the knob is fixedly connected with a threaded column. According to the hemostasis mechanism for the vascular surgery department, during hemostasis, the periphery of a wound can be heated through the heating block across the replacement pad, the temperature of the periphery of the wound reaches 37-42 DEG C, activation of platelets can be accelerated to a certain extent at the temperature, energy metabolism of the platelets is accelerated within the temperature range, and the blood circulation is accelerated. The increase of the concentration of calcium ions in the blood platelets is an important signal of platelet activation, and the increased concentration of the calcium ions can promote the activation of glycoprotein receptors on platelet membranes, so that the binding capacity of the blood platelets and fibrinogen is enhanced, the aggregation of the blood platelets is accelerated, and the hemostasis speed is accelerated.

The invention relates to the technical field of electrochemical sensing, in particular to a multi-channel electrochemical aptamer sensor and a preparation method and application thereof.The multi-channel electrochemical aptamer sensor adopts a commercial silk-screen printing electrode and is combined with a polymer substrate material for amplifying electrochemical signals, and the electrochemical aptamer sensor is prepared. According to the present invention, the corresponding aptamer is fixed by using the gold nanoparticle through the chemical bond so as to specifically detect the three hemorrhage conversion biomarkers after acute cerebral infarction, such as human matrix metalloproteinase (MMP-9), fibrinogen (FN) and plasminogen activation inhibitor (PAI-1); wherein the FN and the PAI-1 are detected through an electrochemical aptamer sensor method for the first time, and simultaneous detection of the three biomarkers is realized through integration with commercial multi-channel micro electrochemical equipment.

The present application relates to a method for preparing and expanding a TSCM cell in vitro. Specifically, the method comprises: 1) obtaining an isolated CD8+ T cell; 2) activating the CD8+ T cell; 3) mixing the activated CD8+ T cell, a fibrinogen solution and a thrombin solution, and culturing same under conditions suitable for cell growth, so as to obtain a TSCM cell culture; and 4) bringing the culture into contact with a dispase so as to obtain a TSCM cell. The present application also relates to a kit for preparing and expanding a TSCM cell in vitro, the use of fibrinogen and thrombin in the preparation of a kit for preparing and expanding a TSCM cell in vitro, and a TSCM cell obtained by means of the method or kit of the present application.

To provide compositions containing RPE cells or RPE monolayers, as well as methods and materials for making RPE cells or RPE monolayers from, for example, stem cells (e.g., iPSCs).SOLUTION: Disclosed is a method for making a retinal pigment epithelium monolayer, the method comprising, or consisting essentially of, culturing stem cells in a container having a surface coated with fibrinogen, wherein the surface is coated with greater than 3 μg / mL of fibrinogen, wherein the cells are in contact with the fibrinogen, and wherein the cells form the retinal pigment epithelium monolayer.SELECTED DRAWING: None

The invention belongs to the technical field of biomedical material engineering, and discloses a hydrogel scaffold used for 3D tumor sphere culture and drug screening, the scaffold is formed by four-arm polyethylene glycol maleimide, fibrinogen and thrombin through Michael addition crosslinking and biopolymerization synergistically, the elastic modulus of 10-1000 Pa can be adjusted by regulating and controlling the proportion of all the components, and the hydrogel scaffold can be used for 3D tumor sphere culture and drug screening. Gel forming is completed within 5 min at the room temperature. The scaffold has the biological activity of fibrous protein and the structural stability of polyethylene glycol, can efficiently support HCT116 cells to form high-activity tumor balls, accurately simulate the in-vivo tumor microenvironment and remarkably improve the accuracy and repeatability of drug screening, is easy and convenient to operate, is compatible with a standard pore plate high-throughput platform and is suitable for drug sensitivity detection of personalized treatment of tumors.

The present invention provides for a novel method for the detection of the presence of myasthenia gravis in a subject, by means of detecting the presence of fibrinogen in serum generated from the blood of the patient. Further novel means for stabilizing the fibrinogen, for the purposes of interrogation of the sample, are provided.

Described herein are multi-specific antigen-binding proteins that bind human fibrin γC or fibrinogen γC domain and vascular endothelial growth factor, and methods of use thereof. In some embodiments, described herein are pharmaceutical compositions comprising the multi-specific antigen-binding proteins that bind fibrin γC or fibrinogen γC domain and vascular endothelial growth factor. In some embodiments, the multi-specific antigen-binding proteins and methods described herein are used for treatment of disorders or conditions of the eye.

The present invention relates to a non-dairy frozen confection comprising mycoprotein biomass in an amount of 0.1 to 2.5 wt%, wherein the mycoprotein biomass comprises mycoprotein fibrils having a fibril length of less than 50 µm.

Disclosed herein are methods of detecting citrullinated polypeptides and / or citrullinated peptides. Citrullinated polypeptides, such as citrullinated vimentin, fibrinogen, and filaggrin, are expressed in various body fluids and / or tissues of individuals with autoimmune diseases, for example the synovium of subjects with rheumatoid arthritis. Further disclosed are methods of diagnosing, treating, selecting an individual for treatment of, and monitoring the treatment of autoimmune diseases, such as rheumatoid arthritis or hidradenitis suppurativa. Kits for use with the methods described herein are also provided.

The invention discloses a special constant-temperature thawing and dissolving device for medical fibrinogen, and relates to the technical field of medical auxiliary instruments, the special constant-temperature thawing and dissolving device for medical fibrinogen comprises a heat preservation box body, and a circulating hot water supply device, a constant-temperature preheating tank, an infusion doser and a dissolving tank which are integrated in the heat preservation box body, the circulating hot water supply device is arranged on one side of an inner cavity of the heat preservation box body; a porous placing frame is placed in the constant-temperature preheating tank, a disposable pipetting mechanism is arranged on the infusion doser, a magnetic shaking table is installed in the center of an inner cavity of the dissolving tank, the preheating function and the dissolving function are integrated into one device, hot water is conveyed to the constant-temperature preheating tank through the circulating hot water supply device for preliminary preheating, and then medicine bottles are transferred to the dissolving tank for dissolving; the process is orderly linked, the tedious step-by-step operation is avoided, the pollution risk is reduced, the operation is simplified, medical fibrinogen thawing and dissolving can be more efficiently and stably completed, and the working efficiency and quality are improved.

A method of detecting inflammation in a subject is provided. The method comprises determining a quantity of amyloid present in fibrin(ogen) protein in a blood sample obtained from the subject, and assigning a level of inflammation in the subject based on the quantity of fibrin(ogen) amyloid determined. A method of determining an effect of a therapeutic treatment on a subject by comparing a pre-treatment level of inflammation with a post-treatment level is further provided.

The invention relates to a freeze drying method of instant fibrinogen, and belongs to the technical field of blood product preparation. The method comprises the steps of plasma pre-fusion, centrifugal separation, FI extraction, FI precipitation, dissolution and filtration, polyethylene glycol purification and separation, S / D inactivation, ethanol purification and separation, glycine purification and separation, ultrafiltration dialysis, preparation, degerming and subpackaging, freeze-drying and capping and the like. Wherein the freeze-drying process comprises four stages of pre-freezing, primary sublimation, desorption drying and plug pressing, and finally dry heat inactivation treatment is carried out. According to the method, efficient extraction and purification of fibrinogen are achieved by accurately controlling the temperature, the time, the pH value and the component proportion of various solutions in each step, and the obtained product has good instant dissolving performance and can be used in the medical fields of clinical hemostasis, tissue repair and the like.

The invention belongs to the technical field of polypeptide synthesis and biological medicine, and particularly relates to a polypeptide PM7 and application thereof in prevention and treatment of thrombus. An original polypeptide is obtained from a transcriptome of salivary glands of poecilobdella manillensis, a truncated polypeptide is intercepted from the original polypeptide, and the truncated polypeptide can be combined with a binding site of fibrinogen of thrombin to intervene the activity of thrombin, so that {d-Phe}-PRP is added at the N end of the truncated polypeptide, and the activity of the thrombin is further intervened. The {d-Phe}-PRP and the truncated polypeptide are connected through a connecting polypeptide, and the polypeptide PM7 is obtained; the polypeptide PM7 has the activity of inhibiting thrombin, the half-life period in vivo is shorter, the safety is high, and the effect of preventing and treating thrombus is achieved.

The invention discloses a preparation method and application of hemostatic peptide with a self-assembly function, and relates to the technical field of biomedical materials. The hemostatic peptide disclosed by the invention has the beneficial effects that the hemostatic peptide has the capability of forming long and uniform nanobelts through self-assembly. The hemostatic peptide is quickly gelated through self-assembly, adsorbs red blood cells and platelets, seals wounds and promotes fibrinogen to be converted into fibrin, so that efficient and quick hemostasis is realized. The haemostatic peptide has high stability, low toxicity and good biocompatibility, can be widely applied to haemostatic scenes of wounds, operations, burns and the like, and provides a novel and efficient haemostatic means for medical first aid and surgical operations.

The present disclosure relates to hemostatic compositions comprising a biocompatible polymer, thrombin, and hemofibrinogen, and methods for preparing such compositions.