7 results about "Autologous platelet" patented technology

The invention provides a mesenchymal stem cell medicine for promoting knee joint cartilage regeneration as well as a preparation method and application thereof, and belongs to the technical field of medicines. The preparation method comprises the following steps: reacting polyethyleneimine with linoleoyl chloride to prepare linoleoyl chloride modified polyethyleneimine for preparing curcumin lipidosome, and reacting with multifunctional peptide to prepare multifunctional lipidosome; the mesenchymal stem cells are mixed with self-assembled RADA16 polypeptide embedded with TGF-beta1 and BMP-6 and subcultured mesenchymal stem cells, the mixture is added into autologous platelet-enriched plasma, and the mesenchymal stem cell medicine for promoting knee joint cartilage regeneration is prepared. According to the invention, curcumin is embedded by lipidosome, mesenchymal stem cells can promote osteogenesis differentiation by using provided nutrients, and interference of inflammatory microenvironment is effectively prevented. Meanwhile, the self-assembled RADA16 polypeptide wraps TGF-beta1 and BMP-6, the synergistic effect is beneficial to regeneration and repair of cartilage tissue, proliferation of bone cells is promoted, and cartilage repair and regeneration are facilitated.

The invention belongs to the technical field of clinical medicine, and relates to a method for extracting autologous platelet-rich plasma, which comprises the following steps of: adding an anticoagulant into an injector I in advance, then extracting autoblood, uniformly mixing for 3-4 times back and forth by virtue of an injector II, then centrifuging for 10 minutes at the speed of 1,500 revolutions per minute, sucking the platelet-rich plasma in the injector I by virtue of an injector III, and extracting the platelet-rich plasma in the injector II by virtue of an injector III. And centrifuging again, and discarding the supernatant by using an injector IV to obtain a high-concentration PRP finished product. According to the extraction method, in the extraction process, blood is uniformly mixed for 3-4 times back and forth between an injector I and an injector II, and the blood is centrifuged for 10 minutes at the speed of 1500 r / min in the first centrifugation process, so that the platelet concentration in the finally extracted PRP is remarkably improved compared with the platelet concentration in peripheral blood; the red blood cells reach the quality control target, the white blood cells are obviously reduced, and a set of standardized autologous platelet-rich plasma clinical extraction method which is simple in extraction device, low in consumable cost, simple to operate and high in extraction concentration is formed.

The invention discloses a blood extraction and centrifugation integrated device in the field of medical instruments, which comprises a tank body, the top of the tank body is communicated with an inlet, the inlet is communicated with a blood transfusion tube, the bottom of the tank body is rotatably connected with a centrifugation structure, the side wall of the bottom of the tank body is communicated with a first outlet and a second outlet, the first outlet is communicated with a blood return tube, and the second outlet is communicated with a separation tube. The inner side wall of the tank body is slidably connected with a partition plate in the vertical direction, the partition plate divides the tank body into a centrifugal chamber and a plasma storage chamber, the centrifugal chamber is communicated with the plasma storage chamber through a siphon, the input end of the siphon is communicated with a valve, a piston of the valve is connected with an elastic rope, and the other end of the elastic rope is fixedly connected with the top of the partition plate. According to the scheme, the functions of centrifugation, separation and blood return are integrated in one tank body, and the whole process is not contacted with air, so that sealed centrifugation, sealed separation and sealed blood return are realized, the blood can be prevented from being polluted, and the safety in an autologous platelet-rich plasma process is ensured.

The invention provides a preparation method of a canine autologous platelet-rich fibrous protein membrane, which comprises the following steps of: collecting canine autologous vein blood by using a vacuum blood collection tube with a preset anticoagulant, centrifuging, discarding supernatant, centrifuging again, discarding lower erythrocytes, and collecting the canine autologous platelet-rich fibrous protein membrane. And placing the obtained dog autologous platelet-rich fibrous protein gel between double stainless steel grids, and pressing to obtain the dog autologous platelet-rich fibrous protein membrane. The invention also provides an application of the prepared autologous platelet-rich fibrin membrane for dogs, wherein the autologous platelet-rich fibrin membrane is used for preparing drugs for wound repair. According to the invention, the autologous platelet-rich fibrin therapy is used for preparing the drug for repairing the dog wound for the first time, and through a triple mechanism of replacing antibiotics by autoimmune regulation, promoting healing by sequential release of growth factors and a zero-allergen biological scaffold, the healing period is shortened while the public health risk is avoided, and the healing effect is improved. The method is an innovative scheme with safety, effectiveness and sustainability.

The invention discloses a disposable handheld synchronous injection type autologous platelet-rich gel wound surface spraying device, and relates to the technical field of medical wound surface treatment equipment.The disposable handheld synchronous injection type autologous platelet-rich gel wound surface spraying device comprises a shell and an anti-skid grab handle which are integrally formed, and a first hose and a second hose which are matched with 10 ml / 1 ml materials are arranged in the shell; stable fixing is achieved through a splayed elastic clamp and an anti-slip strip, the synchronous injection assembly comprises a driving motor, a third synchronous wheel with the wheel ratio being 1: 10 and a matched synchronous wheel, the synchronous injection assembly is combined with an integrally-formed elastic piston piece, rotary motion is converted into precise linear propelling, manual operation is replaced by automatic driving, it is guaranteed that two materials are synchronously conveyed according to the ratio being 10: 1, and the production efficiency is improved. Real-time uniform mixed spraying is achieved through the connecting pipe and the mixed spraying piece, the structure is compact, handheld and portable, the forming stability of the autologous platelet-rich gel can be improved, the labor intensity of medical care and material waste are reduced, and the device is suitable for treating chronic wounds such as diabetic feet and pressure sores.

The described invention provides soft tissue grafts, hard tissue grafts, and composite soft / hard tissue grafts and methods of producing such grafts. The grafts comprise a three-dimensional carrier matrix, a growth factor composition comprising an autologous platelet-rich fibrin and a cell culture composition comprising a culture medium, a population of cells suspended in the culture medium, and cells impregnated on or in a surface of osteoconductive particles.

The implant processing method disclosed in this application includes the following steps: centrifuging blood from a blood collection tube to obtain liquid autologous platelet aggregate, extracting liquid autologous concentrated growth factor from it, and injecting the liquid autologous concentrated growth factor into a liquid autologous concentrated growth factor container; placing an untreated implant into the liquid autologous concentrated growth factor container to immerse it in the liquid autologous concentrated growth factor; and centrifuging the liquid autologous concentrated growth factor and the untreated implant to obtain an implant with its surface covered by the patient's autologous concentrated growth factor, which serves as a surface-treated implant to be implanted in the patient's oral cavity. This application can provide personalized implant processing solutions based on individual patient characteristics, effectively enhancing the integration ability of the implant with bone tissue, promoting new bone formation, and thus improving the implantation outcome.