192 results about "Platelets blood" patented technology

Methods and kits are disclosed to promote vascularization of tissue in mammals using an autologous platelet-rich plasma composition. The kit includes components for the preparation of platelet-rich plasma from the patient's blood and components for administration to an area in need of vascularization. The methods and kits are useful for the treatment of tendinosis, and in particular, lateral epicondylitis and plantar fasciitis.

The present invention discloses one kind of injected bone repairing material and its preparation and application. The injected bone repairing material consists of solution I and solution II, the solution I is the mixture of platelet rich plasma (PRP) and 80-100 mg/ml concentration fibrinogen solution in the ratio of 1 to 3-6; and the solution II is solution mixture of calcium chloride in 40 mmol/ml concentration and thrombin in 400-1000 U/ml concentration. When the injected bone repairing material is used, solution I and solution II in the volume ratio of 1-9 are sucked with two syringes and injected into body to form gel with high adhesive force, with PRP accounting for 10-30 vol%. The injected bone repairing material is used in preparing implant for treating bone defect and bone nonunion and promoting fracture healing.

The invention relates to an extraction method of platelet rich plasma (PRP) and extracted PRP and particularly relates to a method for extracting PRP from blood. The method comprises the following steps of: (a) putting the collected whole blood in a container containing an anticoagulant, and fully and uniformly mixing the blood and the anticoagulant; (b) putting the blood mixed with the anticoagulant in a centrifuge tube, carrying out primary centrifugation, and enabling the blood to be divided into three layers; (c) extracting the uppermost layer and most of the middle layer, transferring the uppermost layer and most of the middle layer into a new centrifuge tube, mixing uniformly and carrying out secondary centrifugation; and (d) discarding the plasma at the upper layer of the centrifuge tube, and resuspending the precipitated plasma by utilizing the remaining plasma, thus obtaining the PRP. The method provided by the invention has the advantages that the operation process is simple, the yield is high and the concentration of platelet in rich plasma is high.

The invention provides a method for building a hollow vascularized heart based on the 3D biological printing technology and the hollow vascularized heart and relates to the technical field of tissue engineering and biology. The method includes: performing reverse modeling to build a heart three-dimensional grid model, importing the data of the heart three-dimensional grid model into a double-nozzle biological printing machine, and driving the nozzles of the printing machine to move according to a predesigned CAD digital model and selected forming parameters, wherein the nozzle 1 of the printing machine is loaded with sacrificial materials and used for printing a support framework, the nozzle 2 of the printing machine sprays biological ink to obtain building bodies, and the effective components of the biological ink comprise hydrogel, platelet-rich plasma, third-generation human umbilical vein endothelial cells and SD rat primary cardiomyocytes; crosslinking and cleaning the building bodies, and performing three-dimensional culture to form the hollow vascularized heart. The method has the advantages that the problem that a large-size hollow vascularized heart is hard in integrated printing, and the hollow vascularized heart built by the method is high in cell activity and has certain functions.

The invention provides a preparation method for a novel biomimetic matrix type biological wound healing material. According to the method, small intestinal submucosa with a three-dimensional scaffold structure is compounded with platelet-rich blood plasma containing a specific proportion of bioactive factors, and a specific process is adopted to carry out modification to form the novel material containing biological induction factors, wherein the novel material has a three-dimensional biomimetic structure. With the biomimetic matrix type biological wound healing material prepared by the method of the present invention, the healing can be promoted, the physiological repair can be promoted, the pathological repair can be inhibited, and the regeneration repair efficiency and the repair quality of the damaged tissue can be improved. The novel biological wound healing material of the present invention can be used for repairs of various human soft tissues, membranous tissues or cavity walls, and for cavity filling, and can be used as the ideal extracellular matrix biomimetic material and the cell scaffold material for wound repairs of the tissues and the organs, promotion of wound healing, prevention of adhesion and the like.

The invention discloses a compound growth factor of blood platelet sources as well as a preparation method and application of the compound growth factor. According to the compound growth factor as well as the preparation method and the application of the compound growth factor, platelet-rich plasma as the raw material is firstly subjected to virus inactivation through an S/D (Solvent/Detergent) method to remove such reagents as organic solvents and eradicators in the platelet-rich plasma and then activated by adding calcium or/and thrombin, supernatant fluid after activation is extracted to obtain the compound growth factor fitting for the natural proportion of the human body; and the problems that single recombinant growth factors in clinic are high in price, and difficult to purify, is involved into gene recombination biological safety and on the like are overcome. The invention further discloses a compound growth factor gel obtained based on the compound growth factor by adding chitosan microspheres carriers, which can slowly release growth factors during use. The compound growth factor has remarkable treatment effects for acute wounds, burns, cosmetic treatment, and chronic hard-healing wounds, is convenient to use, and has high safety.

The invention belongs to the technical field of blood products and particularly relates to a preparation method for platelet rich plasma (PRP). The method comprises a blood collection step, a lymphocyte separation medium treatment step, a first-time centrifugation step, a second-time centrifugation step and the like. According to the preparation method disclosed by the invention, on the basis of the existing PRP preparation technology, firstly, collected blood is treated by adopting a lymphocyte separation medium, and then, by optimizing a centrifugal force and time of centrifugation, the PRP concentration effect of the prepared PRP is further promoted, and the number of red blood cells is greatly reduced; the preparation method has better practical application value.

The present invention relates to a method for separating whole blood into leukocyte-depleted erythrocyte concentrate and plasma. According to the method, in a first step, whole blood is separated into a red blood cell concentrate and plasma in a plasma filter; then, the whole blood is separated into a second red blood cell concentrate during the reverse flow of the whole blood through the same plasma filter and more plasma. The resulting erythrocyte concentrate was mixed with the addition solution. The entire separation system itself is airtight and sterile.

The invention relates to a platelet-rich plasma rapid separating device and a method, and belongs to the field of platelet-rich plasma extracting devices and methods. The device comprises a centrifugal tube; the centrifugal tube comprises an inner tube body, an outer tube body and a sealing cover, wherein the inner tube body is sleeved with the sealing cover; the inner tube body is provided with a shrinking channel, blood is centrifuged, then suction of cell components can be avoided in the mode that the channel is closed through a buckling device, the centrifugal operation procedure is simplified, and the concentration and the preparing efficiency of the platelet-rich plasma after centrifugation are increased; meanwhile, the outer tube body of the centrifugal tube is in a centrifugal-tube shape, the centrifugal tube can be in fit with a universal centrifugal device in the market, and a platelet-rich plasma enrichment device is easy to operate, low in cost and capable of providing a high-concentration and low-cost platelet-rich-plasma and platelet-rich-gel device and method for clinical research.

The invention belongs to the blood product technology field, and discloses a preparation method for leukocyte-depleted platelet rich plasma. The method comprises the following steps: firstly, anticoagulants and blood are drawn and mixed, and whole blood to be separated is obtained; secondly, first centrifugation is carried out, a centrifugate divided into three layers from bottom to top is obtained, and platelet and plasma components in the upper layer are obtained; thirdly, the obtained upper layer components are centrifuged again, and platelet deposition at the bottom and a plasma supernatant are formed; part of the plasma supernatant is drawn, the platelet deposition is subjected to resuspension by the left plasma supernatant, and leukocyte-depleted platelet rich plasma is prepared. The method achieves elimination of leukocytes and enrichment of platelets at the same time, operation is convenient, consumed time is short, the technical indexes of the prepared plasma product are better than technical indexes of present products with the same kind. Centrifugation for two times in the method can be carried out in two centrifuge tubes respectively, or be carried out in one centrifugal device. The invention provides a centrifugal device used for performing the preparation method.

The invention provides a stem cell injection for treating osteoarthritis cartilage defect and preparation and application thereof. The injection comprises fifth-generation umbilical cord mesenchymal stem cells and a liquid solvent, wherein the fifth generation umbilical cord mesenchymal stem cells are suspended in the liquid solvent, and the concentration is (2.0-3.0)x10<7> cells/ 4.5-5.0ml of the liquid solvent. The liquid solvent is normal saline, or autologous platelet-rich plasma, or a mixed solution of normal saline and human albumin, or a mixed solution of normal saline and autologous platelet-rich plasma. The human albumin accounts for 5-8% in volume by percentage. The normal saline accounts for 10% or below in volume by percentage. The injection is administered by intravenous injection or bone joint cavity local injection or the combination of the two, the cell injection amount of the intravenous injection is (1.0-1.5)x10<8> cells/ one time, and the cell injection amount of the bone joint cavity injection is (2.0-3.0)x10<7> cells/ one time/ one knee. The effect of the stem cell injection for treating the integrity degree of osteoarthritis cartilage is obviously superior to that of a traditional hyaluronic acid (HA) method.

The invention belongs to the technical field of preparation of platelet rich plasma, and in particular discloses a method for preparing PRP (Platelet Rich Plasma) through a manual two-step centrifugation method. The method comprises the following steps: centrifuging 25 ml of whole blood mixed with an anticoagulant for 6 minutes at centrifugal force of 800 g for the first time by adopting the two-step centrifugation method, absorbing plasma on the upper layer and on boundary layer in the layered whole blood, and arranging the plasma in an another centrifugal tube; centrifuging for the second time, namely centrifuging for 5 minutes at centrifugal force of 1400 g, and absorbing about 4 ml of the layered plasma on the middle layer to obtain the platelet rich plasma. According to the PRP prepared by the technology, the platelet concentration is 886.33*10<9>/L, the mean corpuscular hemoglobin in the PRP is 27.72 g/L, and the clinical treatment requirements are met.

The human skin filler for injection consists of cell components, blood plasma with rich thrombocyte, blood coagulation factor and calcium ion. Each milliliter of the filler for injection contains cell components in (10-50)x10<6>, thrombocyte in (100-700)x10<6>, blood coagulation factor in 1-10 U, and calcium ion in 10-40 mmol. The cell components are at least one of corium stem fibroblast, fibroblast precursor and fibroblast obtained through acquiring the skin from the patient, extracorporeal no-serum culturing and amplifying, and collecting; the thrombocyte is extracted from the autoblood of the patient; and the blood coagulation factor is autogenous or extraneous. The present invention also provides preparation process of human soft tissue filler for injection. The present invention has the advantages of quick obvious filling and repairing effect, last curative effect, high safety, etc.

This invention relates to a method of reducing residual white blood cells in an apheresed platelet product. The method includes the steps of adding to the platelet product a solution comprising sodium chloride and magnesium and inducing degradation of the residual white blood cells.

The invention discloses a hydrogel loaded with platelet-rich plasma and antibacterial peptide as well as a preparation method and application of the hydrogel. The preparation method comprises the following steps: grafting antibacterial peptide into sodium hyaluronate to form a compound of sodium hyaluronate and antibacterial peptide, then uniformly mixing the compound solution of the sodium hyaluronate and the antibacterial peptide with platelet-rich plasma, and then mixing the obtained mixture with a dextran oxide solution to form the hydrogel loaded with the platelet-rich plasma and the antibacterial peptide. The hydrogel prepared by the invention is biodegradable, has good mechanical properties and biocompatibility, can promote wound healing and tissue regeneration, has a long-acting drug-resistance-free antibacterial effect, and can be applied to wound repair of chronic skin defects.

The invention discloses a platelet-rich gel and a preparation method thereof. The preparation method comprises the following steps that an ethylenediamine tetracetic acid sodium solution is prepared,specifically, sterile water for injection is taken, sodium bicarbonate is added into the sterile water, the pH value of the sterile water for injection is adjusted to 7.8, then ethylenediamine tetracetic acid sodium is added into the mixture and mixed evenly, and the ethylenediamine tetracetic acid sodium solution with the molarity being 0.25 mol/L is prepared; peripheral venous blood of a patientis collected by a container containing the ethylenediamine tetracetic acid sodium solution, centrifugation is conducted, and platelet-rich plasma is prepared; thrombin lyophilized powder and calciumgluconate with the mass concentration being 10% are taken, and the thrombin lyophilized powder and the calcium gluconate are mixed evenly according to the ratio of 1ml of 10% calcium gluconate for every 1000 U of the thrombin lyophilized powder, and a thrombin solution is prepared. The platelet-rich plasma is prepared by mixing the platelet-rich plasma and the thrombin solution according to the volume ratio of 10:1; and the platelet-rich plasma and the thrombin solution are mixed evenly according to the volume ratio of 10:1, and the platelet-rich gel is prepared. According to the platelet-richgel, the therapeutic effect is good, and wound healing can be effectively promoted, and treatment time is shortened.

The invention belongs to the technical field of cosmetology, in particular to a formula of mini-needle nutrient solutions having an autogenous repairing function. The formula of the mini-needle nutrient solutions having the autogenous repairing function comprises the following components in parts by mass: 35 parts to 45 parts of autogenous collagen, and also comprises 35 parts to 45 parts of stemcell suspensions, 3 parts to 8 parts of hyaluromic acid solutions, 3 parts to 8 parts of epidermal growth factor solutions, 3 parts to 8 parts of vitamin E solutions and 3 parts to 8 parts of antioxidant molecules; the formula also comprises 20 parts of platelets-rich plasma, 5 parts of hydroxyapatites, 5 parts of polylactides, 5 parts of polynucleotide, and 10 parts of fibroblast. The formula ofthe mini-needle nutrient solutions having the autogenous repairing function disclosed by the invention has the advantages that the formula is reasonably configured, the functions are varied, the autogenous repairing capability of the skin can be effectively activated, the nutrient solutions are safe and effective, risks are avoided, and the nutrient solutions can be applied from face to body, andare fully coverage of referred items.

The invention provides a preparation reducing wrinkles. The preparation comprises, by weight, 1.5-2.5 parts of platelet rich plasma, 0.1-1 part of sodium hyaluronate and 2-4 parts of stem cell growth factors. According to the preparation reducing the wrinkles, the stem cell growth factors, the sodium hyaluronate and the platelet rich plasma are combined together, effects of promoting cell metabolism and delaying cell aging of the stem cell growth factors and the mechanism of promoting wound healing of the platelet rich plasma are utilized, by means of the mutual matching effect of the stem cell growth factors, the sodium hyaluronate and the platelet rich plasma, texture, collagen and elastic fibers of skin are comprehensively repaired and recombined, the facial wrinkles fade away and are relieved, and therefore the purposes of delaying skin aging, eliminating the wrinkles and resisting aging are achieved. Accordingly, the preparation reducing the wrinkles is high in wrinkle eliminating efficiency and good in wrinkle eliminating effect. The invention further provides a preparing method for the preparation reducing the wrinkles.

Systems, devices, compositions, and methods for treating bleeding, for containing blood that has been lost by a subject due to bleeding, and for treating surfaces contaminated with blood or expected to be contaminated with blood are disclosed. The systems, devices, compositions, and methods utilize platelets, platelet-derived materials, or both. The invention includes sprayers, nebulizers, and equivalents, to deliver platelets and/or platelet-derived materials to desired surfaces. Exemplary embodiments include the use of a pressurized sprayer configured to deliver a topical administration of a lyophilized platelet and/or platelet-derived material to a surface.

The invention relates to a composition for promoting hair growth. The composition includes lyophilized platelet-rich plasma, and further includes one or more of autologous serum, immune cells and vascular matrix components, and further includes one or more of a hair growth nutrient solution and normal saline. The composition is a non-surgical safe and effective hair growth composition, and is usedfor long term without obvious side effects, from the perspective of cytology and biotechnology, growth factors, nutritional components, immune cells and the like are provided, multi-angle, multi-level and multi-dimension improvement of scalp microenvironment can be improved, scalp local microcirculation is promoted, hair follicle stem cells and hair matrix cells are activated, the hair abnormal resting period is shortened, the secretion of sebaceous glands is effectively inhibited, the physiological function of hair follicles is improved, and quick-acting, intermediate-acting, and long-actingpromotion of hair continuous regeneration is achieved.