93 results about "Platelets blood" patented technology

The utility model discloses a platelet-rich plasma preparation device, which belongs to the technical field of medical instruments and comprises an upper end jacket, one end of the upper end jacket is connected with an upper end cover, the other end of the upper end jacket is connected with a lower end jacket, the bottom of the lower end jacket is connected with a lower end cover capable of moving up and down, and the upper end jacket is provided with a first cavity. A second chamber, a third chamber and a connecting channel for communicating the second chamber with the third chamber are sleeved outside the lower end part, and the second chamber is communicated with the first chamber; the upper end cover is provided with an upper sealing rod for controlling the first cavity to be communicated with or disconnected from the second cavity, the lower end cover is provided with a lower sealing rod for controlling the second cavity to be communicated with or disconnected from the third cavity, the upper sealing rod is provided with a fourth cavity, and a limiting rod capable of moving up and down along the inner wall of the fourth cavity is arranged in the fourth cavity. The PRP preparation device is convenient to operate, high in preparation efficiency and capable of preparing PRP with different leukocyte contents according to requirements, and different treatment requirements are met.

A system and method of isolating extracellular vesicles. The method includes loading one or more of blood or bone marrow into an input port of a concentration system and centrifuging one or more of the blood or bone marrow to separate one or more of red blood cells, platelet poor plasma, or platelet rich plasma / bone marrow concentrate fractions via a centrifuge device. The method further includes pumping one or more of bone marrow / platelet rich plasma fractions and platelet poor plasma fractions into a receptacle of the concentration system and adding a concentrated aqueous two-phase solution to one or more of the bone marrow concentrate / platelet rich plasma fractions and platelet poor plasma fractions. The method also includes drawing the concentrated aqueous two-phase solution and one or more of the bone marrow concentrate / platelet rich plasma fractions or platelet poor plasma fractions back into the centrifuge device to isolate one or more of extracellular vesicles and platelet rich plasma / bone marrow concentrate fractions.

A platelet-rich plasma (PRP) activation system for activating PRP contained in a container includes a housing, a power supply, a piezoelectric transducer array, a support structure, and a coupling medium. The power supply is located in the housing. The piezoelectric transducer array is operably connected to the power supply and is located in the housing. The piezoelectric transducer array is configured to generate a focused shock wave using power from the power supply. The support structure is operably connected to the housing and is configured (i) to receive the container, and (ii) to position the container at least partially within the housing, such that at least a portion of the PRP is located at a focal volume formed by the focused shock wave. The coupling medium is located in the housing and is positioned between the piezoelectric transducer array and the container.

The invention relates to the technical field of hydrogel, in particular to bioactive bionic gradient hydrogel as well as a preparation method and application thereof.The preparation method comprises the following steps: dissolving quaternary ammonium salt chitosan, 3-acrylamidophenylboronic acid, platelet-rich plasma and phenyl (2, 4, 6-trimethylbenzoyl) lithium phosphate in deionized water, stirring until complete dissolution, centrifuging, and drying to obtain a hydrogel solution containing chitosan, 3-acrylamidophenylboronic acid, platelet-rich plasma and phenyl (2, 4, 6-trimethylbenzoyl) lithium phosphate; pre-polymerization liquid is obtained; preparing a tannic acid-iron ion (TA-Fe < 3 + >) mixed solution; adding the pre-polymerized liquid into a mold, and carrying out ultraviolet radiation crosslinking to obtain hydrogel; the hydrogel part is soaked in a tannic acid-iron ion mixed solution, and the gradient hydrogel is obtained. According to the biologically active bionic gradient hydrogel as well as the preparation method and the application thereof, the dual-network hydrogel is soaked in a TA-Fe < 3 + > solution to form a TA-Fe < 3 + > coordinated concentration gradient, so that the hard-to-soft mechanical gradient of tissues such as bone-cartilage, rotator cuff, achilles tendon and the like is accurately simulated.

The invention provides a freeze-dried universal platelet rich plasma (PRP) preparation and a preparation process thereof, aiming at solving the problems that the existing PRP is limited in blood type matching, difficult in biological activity preservation, short in validity period and the like. According to the preparation, 18-19% of the type A, 7-8% of the type B and 73-74% of the type AB are mixed with PRP of different blood types and freeze-dried. Tests show that agglutination does not exist in cross blood matching, the titers of the anti-A antibody and the anti-B antibody are both lower than 1: 2, and blood group reaction is effectively avoided. Freeze-drying causes little damage to growth factors, still has biological activity within 24 months, and has a good cell proliferation test effect. Animal experiments show that wound healing can be remarkably promoted. In addition, the culture medium is good in stability in the environment of 4 DEG C, and the moisture, growth factors and the sterile state all meet the requirements. The preparation disclosed by the invention has the advantages of obvious universal advantages, good biological activity preservation and strong stability, and provides a better choice for clinical treatment.

Disclosed herein is a composition and use of the composition in a method for penis enlargement; the composition comprises hyaluronic acid and platelet-rich plasma, and the hyaluronic acid and the platelet-rich plasma are present in the composition in a ratio of about 1: 2.

The present application belongs to the field of photochemical therapy, and particularly relates to a method for photochemical treatment of riboflavin based on multi-wavelength LED and application thereof in treatment of graft-versus-host disease. The present application selects different wavelength LED combinations as light sources to optimize the riboflavin photochemical method, controls the light intensity at 1000-25000 μW / cm², controls the irradiation time at 5-15 minutes, and controls the riboflavin concentration at 20-150 μM, thereby significantly improving the photochemical reaction effect. The method can efficiently induce PBMC apoptosis in a plasma environment, significantly reduces the levels of key pro-inflammatory cytokines such as TNF-α, IL-6 and IFN-γ, and has no significant influence on platelets, hemoglobin and blood clotting function, thereby providing a safe and controllable new cell preparation method for GVHD treatment.

This invention provides a platelet-rich plasma (PRP) sustained-release collagen sponge scaffold and its preparation method. The scaffold comprises: a first collagen sponge layer, a porous collagen sponge layer, and a second collagen sponge layer stacked sequentially; the porosity of the first and second collagen sponge layers is no more than 40%, and the pore size is no greater than 20 μm; the porosity of the porous collagen sponge layer is no less than 70%, and the pore size is no less than 50 μm; at least one sustained-release cavity is disposed in the porous collagen sponge layer for loading platelet-rich plasma gel. Through the structural design of the gradient pores of the collagen sponge and the internal sustained-release cavity, the outer layer forms a physical barrier to effectively prevent PRP loss; the inner layer efficiently stores PRP, ensuring the loading of effective components; the high porosity of the inner layer allows the PRP in the sustained-release cavity to slowly permeate outward, achieving long-term sustained release of PRP growth factors; at the same time, the pores of the inner layer can also provide sufficient space for cell migration and proliferation, promoting tissue regeneration.

The platelet-rich plasma centrifugal device comprises a centrifugal support body, a first positioning part and a second positioning part are movably arranged on the centrifugal support body, and a fixing part is arranged on the centrifugal support body; the fixing part fixes a needle head of a blood taking needle, the second positioning part fixes a needle body of the blood taking needle, and the first positioning part fixes a piston of the blood taking needle.

The invention discloses application of a mesenchymal stem cell combined platelet in preparation of a preparation for treating ankle arthritis, the mesenchymal stem cell combined platelet (platelet-rich plasma) is adopted to treat the ankle arthritis, the operation is simple, the side effect is small, the treatment effect is remarkable, the treatment period is short, and the application is safe and reliable. The platelet-rich plasma is rich in growth factors, can promote the regeneration of damaged tissue vessels, increase blood supply and nutrient substances, enhance the biological activity and migration ability of cartilage cells, accelerate the healing of damaged tissues around ankle joints, and effectively promote the growth and reproduction and osteogenic differentiation of bone marrow mesenchymal stem cells (BM-SCs). The method has the advantages that the cartilage differentiation of the mesenchymal stem cells at the joints is promoted, the cell repair and regeneration of the affected parts of the joints are realized, the functional recovery of the joints is promoted, and the ankle arthritis is treated more efficiently.

The invention belongs to the technical field of clinical medicine, and relates to a method for extracting autologous platelet-rich plasma, which comprises the following steps of: adding an anticoagulant into an injector I in advance, then extracting autoblood, uniformly mixing for 3-4 times back and forth by virtue of an injector II, then centrifuging for 10 minutes at the speed of 1,500 revolutions per minute, sucking the platelet-rich plasma in the injector I by virtue of an injector III, and extracting the platelet-rich plasma in the injector II by virtue of an injector III. And centrifuging again, and discarding the supernatant by using an injector IV to obtain a high-concentration PRP finished product. According to the extraction method, in the extraction process, blood is uniformly mixed for 3-4 times back and forth between an injector I and an injector II, and the blood is centrifuged for 10 minutes at the speed of 1500 r / min in the first centrifugation process, so that the platelet concentration in the finally extracted PRP is remarkably improved compared with the platelet concentration in peripheral blood; the red blood cells reach the quality control target, the white blood cells are obviously reduced, and a set of standardized autologous platelet-rich plasma clinical extraction method which is simple in extraction device, low in consumable cost, simple to operate and high in extraction concentration is formed.

The present invention addresses the problem of providing: a method for producing platelet-rich plasma capable of achieving a stable and high recovery rate when collecting platelets from a blood sample; and platelet-rich plasma produced by the method. The invention relates to a method for preparing platelet-rich plasma, which comprises the following steps of: A, carrying out centrifugal separation on a blood sample of a mammal for 1-30 minutes under the condition of 100-1000G, and recovering supernate; and step B, carrying out centrifugal separation on the residual blood sample after the supernatant is recovered in the step A under the condition of 100-1000G for 1-30 minutes, and recovering the supernatant, so as to obtain the platelet-rich plasma.

The objective is to provide a method that suppresses the formation of pseudopods of platelets during the preparation of PRP, thereby preventing the formation of aggregates. [Solution] The method for producing platelet-rich plasma (PRP) according to the present invention is characterized by adding a local anesthetic to the blood when producing PRP.

The platelet-rich plasma preparation device comprises a main body, a storage cavity, a separation cavity, a rotary handle, an upper cover and a lower cover, the storage cavity and the separation cavity are located in the main body, the upper end of the separation cavity is tightly covered and sealed through the upper cover, the lower cover is arranged below the storage cavity, the rotary handle is connected to the bottom end of the storage cavity in a matched mode and used for pushing layered blood in the storage cavity to move from bottom to top, and the separation cavity is internally provided with a plurality of cavities capable of being rotationally switched; when the separation cavity is communicated with the storage cavity, all layers of centrifuged whole blood are sequentially pushed into all cavities corresponding to the separation cavity through the rotating handle. The separator is reliable in structure and good in use performance, a platelet-rich layer, a plasma layer and a red blood cell layer can be completely and independently separated and stored in one separator efficiently, safely, stably and visually through simple rotating operation, red blood cells can be effectively prevented from being mixed into platelets, the recovery rate and efficiency of the platelets are improved, meanwhile, the sealing performance is good, and the service life is long. And mutual pollution between blood and the outside is prevented.

Described herein are methods and systems for isolating exosomes or extracellular vesicles from a blood sample. In one embodiment, the method includes separating and fractionating whole blood and cleaving platelets and leukocytes using beads, thereby releasing exosomes or extracellular vesicles. The obtained exosome-rich platelet rich plasma (ER-PRP) can be further refined through microfiltration.

The invention discloses a whole blood leukocyte-depleted multi-component double-filter blood united bag which comprises a main collection bag, a red blood cell storage bag, a plasma storage bag, a platelet-rich plasma storage bag, and a main collection pipeline, a first transfer pipeline and a second transfer pipeline which are communicated with one another. The core improvement lies in that the first transfer pipeline is provided with a first leukocyte-depleting filter special for red blood cells, and the second transfer pipeline is provided with a second leukocyte-depleting filter special for platelet-rich plasma. The double filters solve the problem that a single filter cannot give consideration to characteristics of different blood components, and synchronous and optimized leukocyte removal treatment of red blood cells and platelet-rich plasma in a closed system is realized. Besides, a sampling pipeline, an anti-needling protection device, a bypass pipeline, an SAG-M additive bag and the like are integrated in the linked bag, so that the blood component yield and activity are improved, and the operation safety and the flow flexibility are guaranteed at the same time.

Described herein are novel methods for fractionating and isolating platelets, platelet- and extracellular vesicle-derived growth factors, exosomes, globulins, fibrinogen and albumin, and methods of using the isolated platelets, platelet and extracellular vesicle-derived growth factors, exosomes, globulins, fibrinogen and albumin for regenerating tissue in a subject, treating fibrinogenemia or a clotting deficiency in a subject, treating ischemia and hypoxia, treating dry-eye syndrome, an orthopedic disorder, or a dental disorder in a subject. Also described herein are growth media for culturing mammalian (e.g., human) cells.

Syringes and syringe systems for use in centrifuge machines including coupling arms for engaging the centrifuge machines. The syringe including a housing with a first coupling arm extending from a first side of the housing and a second coupling arm extending from a second side of the housing. The first side is opposite the second side. The syringe also includes at least one plunger slidingly received within the housing. Methods of using the syringes and syringe system for extracting and injecting platelet-rich plasma are also disclosed.

The utility model relates to a device for preparing platelet-rich plasma with less blood volume. The device comprises a preparation tube unit, an upper tube cover unit, an isolation unit and a lower tube cover unit, the platelet-rich plasma preparation device has the advantages that by changing the structure of the preparation tube unit, the platelet-rich plasma preparation device can be suitable for treatment of small animals or pets; the volume of blood contained in the preparation tube unit is reduced, so that the centrifugation time is shortened, and the risks of infection and blood coagulation are reduced; besides, after the blood is centrifugally separated, a lower tube cover unit is arranged and is in threaded connection with a preparation tube unit, so that the lower tube cover unit can be rotated to adjust the volume of a fourth cavity of the preparation tube unit, the buffy yellow layer by layer is positioned in a third cavity, and the plasma is completely positioned in a second cavity of the preparation tube unit; therefore, subsequent extraction by medical staff is facilitated.

The utility model relates to the field of biomedical technology, concretely is a kind of platelet-rich plasma preparation device.The device includes storage tube, sealing cover and piston assembly.Storage tube is divided into storage part and injection part, and stop structure is arranged in storage cavity;Sealing cover is in interference fit with injection part and can be punctured, and closed blood sampling is realized in combination with vacuum negative pressure cavity.Piston assembly realizes locking and release by the cooperation of elastic movable head and stop structure, ensures that each blood layer is independently pushed after centrifugal stratification, and avoids cross contamination.Storage tube is made of transparent material and is provided with scale, so that stratification state and accurate separation can be observed.The device is integrated with blood sampling, centrifugation and extraction functions, and PRP preparation can be completed through single centrifugation and push-pull piston, which is convenient to operate and high in efficiency.The utility model solves the problems of complex operation, low separation efficiency and easy pollution of traditional method, and significantly improves the reliability and practicality of PRP preparation.

The invention discloses an osteochondral integrated sponge scaffold as well as a preparation method and application thereof, and belongs to the field of osteochondral repair materials. The preparation method comprises the following steps: (1) mixing a collagen dispersion liquid and a polyvinyl alcohol solution, then adding a phosphate buffer solution and beta-tricalcium phosphate, and then obtaining lower-layer hydrogel through a pouring method; (2) mixing the collagen dispersion liquid with a polyvinyl alcohol solution, then adding a phosphate buffer solution, and then obtaining upper-layer hydrogel through a pouring method; and (3) pouring the upper-layer hydrogel on the lower-layer hydrogel, constructing a sponge scaffold through a multi-time freeze-thawing method and a freeze-drying method, and finally soaking the sponge scaffold in thrombin to obtain the osteochondral integrated sponge scaffold. The scaffold can adsorb platelet-rich plasma, has certain adhesion capacity, can realize in-situ fixation and biological integration at a defect part, can well recover physical and biological dysfunctions of bone joints and osteochondral bones, and provides a potential application idea for clinical treatment of osteochondral defects.

The invention discloses a blood extraction and centrifugation integrated device in the field of medical instruments, which comprises a tank body, the top of the tank body is communicated with an inlet, the inlet is communicated with a blood transfusion tube, the bottom of the tank body is rotatably connected with a centrifugation structure, the side wall of the bottom of the tank body is communicated with a first outlet and a second outlet, the first outlet is communicated with a blood return tube, and the second outlet is communicated with a separation tube. The inner side wall of the tank body is slidably connected with a partition plate in the vertical direction, the partition plate divides the tank body into a centrifugal chamber and a plasma storage chamber, the centrifugal chamber is communicated with the plasma storage chamber through a siphon, the input end of the siphon is communicated with a valve, a piston of the valve is connected with an elastic rope, and the other end of the elastic rope is fixedly connected with the top of the partition plate. According to the scheme, the functions of centrifugation, separation and blood return are integrated in one tank body, and the whole process is not contacted with air, so that sealed centrifugation, sealed separation and sealed blood return are realized, the blood can be prevented from being polluted, and the safety in an autologous platelet-rich plasma process is ensured.

The utility model discloses a one-time centrifugation blood platelet-rich plasma preparation device, which comprises a device main body, an upper cover and a red blood cell storage cover, the device main body is in a shape of tightening towards the center, the tightening part forms a blood channel and is provided with a rotary switch, and a platelet plasma storage chamber is arranged above the rotary switch; a suction hole is formed in the platelet plasma storage chamber, an erythrocyte storage chamber is arranged below the rotary switch, and the connecting depth of the erythrocyte storage cover and the device body can be adjusted to change the capacity of the erythrocyte storage chamber. The operation process can be remarkably simplified through the single-time centrifugal technology, the personal error risk caused by multiple times of operation is reduced, and the method is particularly suitable for clinical rapid processing or medical scenes with limited resources. In addition, mechanical damage to platelets and growth factors can be effectively reduced by reducing the number of times of centrifugation, higher cell activity and functions can be reserved, and therefore the treatment effect of the PRP is further improved.

The utility model belongs to the technical field of centrifugal equipment, and particularly relates to an auxiliary PRP extraction device. Comprising a base, a connecting rod is connected to the top of the base, and a support is arranged at the top of the connecting rod; a lower mounting groove and an upper mounting groove which are coaxial in the vertical direction are respectively formed in the base and the support, and injectors with conventional sizes can be accommodated in the lower mounting groove and the upper mounting groove; the device further comprises a spiral push rod capable of moving up and down in the vertical direction relative to the upper mounting groove. The auxiliary PRP extraction device provided by the utility model can be directly matched and applied to a specific centrifugal machine, a spiral pushing means is adopted, the pushing pressure impact force can be reduced, and the damage of pressure to active cell components is reduced, so that the extraction dosage is effectively controlled, the extraction rate can be ensured, and the extraction efficiency is improved. The concentration of platelet-rich plasma is accurately controlled, and the treatment effect is finally guaranteed.

The utility model relates to medical equipment technical field discloses a kind of platelet-rich plasma separators, comprising: round plate, the outer surface of the round plate is fixedly installed with limit block.This kind of platelet-rich plasma separator, due to the design of movement mechanism, reach the flexible adjustment effect of centrifugal radius without replacing centrifugal cup, can be according to different patient blood viscosity, hematocrit and other indexes, accurately control centrifugal force, avoid platelet residue or excessive activation, significantly improve platelet-rich plasma preparation quality and biological activity.Discard the cumbersome operation of traditional replacement centrifugal cup, substantially improve operation efficiency, while reducing the mismatch risk caused by frequent replacement of spare parts, reduce the operation burden of medical staff, reduce equipment spare parts procurement cost and management difficulty, effectively improve the versatility and utilization of equipment, reduce hospital equipment procurement and maintenance cost.

This application relates to the field of medical devices, specifically to a platelet-rich plasma (PRP) separation and preparation stent, comprising: a support frame; a conduit horizontally mounted on the support frame; a first three-way valve mounted on the support frame; a first snap-fit ​​mounted on the support frame; and a first retaining ring fixedly mounted on the support frame. This PRP separation and preparation stent addresses the problem that hand movement during injection using a syringe containing stratified blood can cause mixing of different blood layers within the syringe, leading to a decrease in the purity of the extracted PRP. It improves the purity of the extracted PRP, and is simple and easy to use.

The utility model discloses a platelet rich plasma preparation device which comprises a vacuum tube, a permeation tube and a pipeline communicated with the vacuum tube and the permeation tube, the vacuum tube is a cylindrical hollow tube, the top of the vacuum tube is provided with a pressing plug capable of being pressed, and the pressing plug is provided with an area for inserting a blood drawing needle. A first membrane assembly with the aperture of 0.2-5 microns is arranged in the vacuum tube, and the first membrane assembly comprises a first membrane layer, a coating layer arranged on the bottom surface of the first membrane layer and a nylon net arranged at the bottom of the coating layer; the permeation tube is U-shaped, an inlet and an outlet are respectively arranged at two ends of the U-shaped permeation tube, a hollow fiber membrane is arranged in the permeation tube, the hollow fiber membrane is bent to have a radian consistent with the appearance of the permeation tube, two ends of the hollow fiber membrane are respectively connected with the inlet and the outlet, and the molecular weight cut-off of the hollow fiber membrane is 2-40,000 Daltons. A waste discharge port is formed in the permeation pipe. According to the utility model, the preparation efficiency is improved, and high-concentration PRP (platelet rich plasma) can be obtained under the condition of less blood consumption.

The application discloses a hydrogel for endometrial repair, which is prepared from platelet-rich plasma, benzene boronic acid modified hyaluronic acid methacrylate (HAMA-PBA), phenyl-2,4,6-trimethyl benzoyl phosphonic acid lithium (LAP), calcium chloride (CaCl2) and polyvinyl alcohol (PVA). The application further provides a preparation method of the hydrogel, which comprises the following steps: mixing HAMA-PBA aqueous solution, LAP aqueous solution and CaCl2 aqueous solution to obtain a precursor solution A, dissolving PVA in PRP to obtain a PVA-PRP mixed solution as a precursor solution B, mixing the precursor solution A and the precursor solution B at a volume ratio of 1:1 to obtain PRP@HAMA-PBA-PVA hydrogel, and irradiating the PRP@HAMA-PBA-PVA hydrogel under ultraviolet light to obtain the hydrogel for endometrial repair. The hydrogel can significantly promote endometrial cell proliferation, has strong pro-angiogenic characteristics, inhibits endometrial fibrosis, and thus comprehensively repairs the endometrial microenvironment, promotes endometrial regeneration and improves embryo implantation rate and live birth rate.

1. The name of the design product: blister box (platelet-rich plasma preparation set). 2. The use of the design product: used for integrated packaging of platelet-rich plasma set, anti-pressing and shock-proofing of platelet-rich plasma preparation set during transportation to prevent extrusion or damage during transportation. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: front view.