213 results about "Platelets blood" patented technology

The invention relates to the technical field of biological medicine, in particular to a wound repair material and a preparation method and application thereof.The wound repair material is in a gel shape and comprises hydrogel and platelet-rich plasma, the hydrogel is formed by dynamic crosslinking of collagen and oxidized hyaluronic acid, and the hydrogel physically encapsulates the platelet-rich plasma. The gelatinous wound repair material provides bionic mechanical support through a dynamic cross-linked network, synergistically regulates and controls an inflammation microenvironment and sequential release of PRP growth factors, remarkably promotes angiogenesis and wound healing, is suitable for the clinical treatment fields of diabetic ulcer, chronic difficult-to-heal wounds and the like, and has broad application prospects. The material has the functions of mechanical support, anti-inflammatory regulation and growth factor controlled release.

The present invention relates to the field of biomedicine and discloses a platelet-rich plasma for anti-aging and promoting tissue repair and regeneration, a preparation method, and an application thereof, comprising the following steps: obtaining concentrated platelet plasma, and treating the concentrated platelet plasma with low-intensity pulsed ultrasound; the treatment conditions are one of the following treatment methods: 1) the treatment power is 2 to 5 W / cm 2 , the pulse period is 50%, and the ultrasonic treatment time is 2~10 min; 2) First, the first ultrasonic treatment is performed: using 0.2~1.0 W / cm 2 , the pulse period is 50%, ultrasonic treatment is 1~5 min; then the second ultrasonic treatment is carried out: 1.5~5.0 W / cm 2 , the pulse period is 50%, and the ultrasonic treatment is 1 to 5 minutes; the present invention adopts a special ultrasonic treatment system to obtain a PRR product with special activation and lysis characteristics. The product has active substances that are beneficial to tissue repair and regeneration and anti-aging, and shows excellent effects in promoting cell proliferation, cell migration and wound healing.

The invention provides a composition for beautifying, which comprises a blood platelet concentrated solution prepared by differentiation of human pluripotent induced stem cells (iPSC), and an additive for preparing a blood platelet suspension or gel, and is characterized in that the composition for beautifying comprises the following components: a blood platelet concentrated solution prepared by differentiation of human pluripotent stem cells (iPSC), and an additive for preparing the blood platelet suspension or gel. The invention aims to provide a functional platelet-rich composition which can be prepared in a large scale, is stable in batch and can be produced in a standardized manner in the aspect of promoting wound healing. According to the composition, the platelet concentrate and platelet-rich plasma (PRP) separated from blood in the composition are completely different in component, the component is stable, and the slow-release growth factor is beneficial to tissue repair.

The invention relates to the technical field of hydrogel, in particular to composite hydrogel capable of stopping bleeding, resisting bacteria and promoting alveolar bone repair, a preparation method and application. The composition comprises a polyacrylic acid solution, a calcium chloride solution, a disodium hydrogen phosphate solution, platelet-rich plasma (including plasma, platelet lysis buffer, platelet-rich fibrin and fibrin of high-concentration concentrated growth factors) and nano clay (hectorite). The preparation method has the advantages of simple process, easily available raw materials, environment-friendly production process, safety and short preparation period. The composite hydrogel is good in biocompatibility and injectable, and can be widely applied to the field of biomedical materials.

The invention relates to the technical field of biological medicine, and discloses anti-aging and tissue repair and regeneration promoting platelet-rich plasma and a preparation method and application thereof.The preparation method comprises the following steps that concentrated platelet plasma is obtained, and low-intensity pulse ultrasound is adopted for treating the concentrated platelet plasma; the treatment conditions are one of the following treatment modes: 1) the treatment power is 2-5 W / cm < 2 >, the pulse period is 50%, and the ultrasonic treatment time is 2-10 min; (2) first ultrasonic treatment is carried out, specifically, 0.2-1.0 W / cm < 2 > is adopted, the pulse period is 50%, and ultrasonic treatment is carried out for 1-5 min; carrying out second ultrasonic treatment for 1-5 minutes by adopting 1.5-5.0 W / cm < 2 > and a pulse period of 50%; a special ultrasonic treatment system is adopted, the PRR product with special activation and lysis characteristics is obtained, and the product contains active substances beneficial to tissue repair and regeneration and anti-aging, and shows excellent effects in the aspects of promoting cell proliferation, cell migration and wound healing.

The invention relates to a therapeutic drug, in particular to a construction method and application of a cell factor sustained-release complex combined with stem cells, and the cell factor sustained-release complex can be used for preventing intrauterine adhesion and promoting endometrial repair. The invention aims to provide a construction method and application of a cell factor slow-release complex combined with stem cells, so that the cell factor slow-release complex is effectively applied to repair of intrauterine adhesion and endometrium. According to the technical scheme, the construction method of the cell factor slow-release complex combined with the stem cells comprises the following steps: (1) preparing platelet-rich plasma, activating and enriching platelet-rich plasma cell factors, and adsorbing the released cell factors through hydrogel; (2) preparing a vascular extracellular matrix by using a decellularization technology, wherein the vascular extracellular matrix is used for adsorbing platelet-rich plasma cytokines; (3) acquiring umbilical cord mesenchymal stem cells; constructing an umbilical cord mesenchymal stem cell / extracellular matrix slow-release complex; and (4) preparing the cell factor slow-release complex combined with the stem cells.

The invention discloses a platelet-rich plasma collection quantity evaluation method, and relates to the technical field of plasma collection quantity evaluation, and the method adopts real-time dynamic prediction and closed-loop control, and upgrades a traditional evaluation mode of statically detecting platelet concentration and leukocyte mixing quantity only after collection to real-time quality monitoring controllable in the whole process. The consistency and the accuracy of platelet rich plasma (PRP) collection are obviously improved; continuous collection data are smoothed and predicted through a Kalman filtering algorithm, the problem of over-collection or under-collection caused by manual judgment or a fixed threshold value in a traditional method is avoided, and the plasma quality and the stability of the curative effect are improved. A dynamic evaluation mechanism effectively reduces the synchronous enrichment risk of leukocytes in the PRP collection process; in an existing automatic collecting device, the leukocyte content and the platelet concentration are increased synchronously, so that pro-inflammatory factors in PRP are excessive, and tissue repair is inhibited.

The invention discloses a high-purity P-PRP preparation system and a preparation method thereof. The high-purity P-PRP preparation system comprises a sterile syringe for sampling, a centrifugal machine for providing a centrifugal effect, a centrifugal sleeve and a separator for purifying a sample of the centrifuged sterile syringe, the centrifugal sleeves are of two types and comprise an inverted centrifugal sleeve used for first-time centrifugation and an upright centrifugal sleeve used for second-time centrifugation. The preparation method comprises the steps of sample preparation, primary centrifugation, purification and re-sampling, secondary centrifugation, final sampling and the like, and finally the leukocyte-depleted platelet-rich plasma with high purity is obtained. According to the system and the method, a conventional medical general syringe can be directly used for the closed centrifugal sleeve, the problem that the syringe makes contact with the outside in the transferring and centrifuging process is effectively solved, the pollution risk in the PRP preparation process is reduced, meanwhile, P-PRP is conveniently extracted, the influence of liquid hanging on the wall on purity is avoided, the purity is improved, and residual pollution is rejected.

The utility model discloses a leukocyte-rich PRP (platelet rich plasma) extraction device. The centrifugal tube comprises a centrifugal tube body and a tube cap which are buckled with each other, the centrifugal tube body sequentially comprises a first cavity, a second cavity and a third cavity from top to bottom, the first cavity, the second cavity and the third cavity are communicated in sequence, and the tube inner diameter of the second cavity is smaller than that of the third cavity; the piston is embedded in the third cavity in a manner of moving up and down; the separation device is arranged in the third cavity in a penetrating manner; the separation device moves in the length direction of the second cavity so as to communicate or close the second cavity and the third cavity; according to the scheme, the leukocyte-rich platelet-rich plasma (LR-PRP) extraction device is efficient and easy and convenient to operate, high-concentration platelets and leukocytes can be effectively separated and extracted in single operation, the operation steps are simplified, the pollution risk is reduced, the extraction efficiency and the sample activity are improved, and therefore clinical requirements are better met.

The invention provides autologous platelet-containing plasma gel and a preparation method and application thereof, and relates to the technical field of biological medicine and tissue engineering.The preparation method comprises the following steps that peripheral venous blood of a patient is extracted and added into a centrifugal tube mixed with a sodium citrate anticoagulant; putting the centrifugal tube into a centrifugal machine for centrifuging, and taking upper-layer plasma; wherein the upper-layer plasma refers to plasma between the upper-layer liquid level and the lower-layer red blood cell layer by 1mm; the centrifugal speed ranges from 1300 r / min to 1700 r / min; the centrifugation time is 3-5 min; under a sterile condition, preparing a calcium gluconate / thrombin mixed solution; and dropping the calcium gluconate / thrombin mixed solution into the upper plasma, mixing, and solidifying to obtain the autologous platelet-containing plasma gel. After one-time centrifugation, upper-layer full-layer plasma is extracted, the volume of the prepared gel is far larger than that of a traditional method, the utilization rate of whole blood platelets and fibrinogen is close to 100%, and regeneration and repair of large-area tissue wounds can be met.

The invention relates to glucose and pH dual-response hydrogel capable of intelligently responding to release of platelet-rich plasma, preparation and application, and belongs to the field of cellulose materials.Chitosan is alkalized and then subjected to carboxymethylation treatment, and carboxymethyl chitosan is obtained; the preparation method comprises the following steps: oxidizing nano cellulose to obtain hydroformylation modified nano cellulose; carboxymethyl chitosan and hydroformylation modified nano cellulose are mixed, glucose oxidase, platelet-rich plasma and nano cerium oxide particles are added in the mixing process, and the glucose and pH dual-response hydrogel capable of intelligently responding to release of platelet-rich plasma is obtained. According to the invention, a cross-linking agent is not needed, the operation is simple, the cost is low, the hydrogel can be rapidly formed, and the hydrogel can be automatically and gradually dissolved to release micromolecular substances wrapped in the hydrogel after being stimulated by glucose and pH change, is environment-friendly, has good biocompatibility, and is beneficial to promoting the application of the hydrogel in the fields of diabetes wound dressings and the like.

The utility model discloses a platelet-rich plasma preparation device, which belongs to the technical field of medical instruments and comprises an upper end jacket, one end of the upper end jacket is connected with an upper end cover, the other end of the upper end jacket is connected with a lower end jacket, the bottom of the lower end jacket is connected with a lower end cover capable of moving up and down, and the upper end jacket is provided with a first cavity. A second chamber, a third chamber and a connecting channel for communicating the second chamber with the third chamber are sleeved outside the lower end part, and the second chamber is communicated with the first chamber; the upper end cover is provided with an upper sealing rod for controlling the first cavity to be communicated with or disconnected from the second cavity, the lower end cover is provided with a lower sealing rod for controlling the second cavity to be communicated with or disconnected from the third cavity, the upper sealing rod is provided with a fourth cavity, and a limiting rod capable of moving up and down along the inner wall of the fourth cavity is arranged in the fourth cavity. The PRP preparation device is convenient to operate, high in preparation efficiency and capable of preparing PRP with different leukocyte contents according to requirements, and different treatment requirements are met.

The utility model discloses a blood platelet rich plasma preparation device which comprises a middle sleeve, an upper storage body, an upper storage cover, a lower storage body and a lower storage cover, a hyperbolic PRP channel and a hyperbolic red blood cell channel are arranged in the upper storage body and the lower storage body respectively, an upper locking bolt and a lower locking bolt are fixed in the middle sleeve, and after the upper locking bolt and the PRP channel are locked in a matched mode, the upper locking bolt and the lower locking bolt are locked in a matched mode. The upper storage body and the upper storage cover form a PRP storage chamber, after the lower locking bolt and the red blood cell channel are matched and locked, the lower storage body and the lower storage cover form a red blood cell storage chamber, the middle sleeve is a plasma storage chamber, a PRP suction opening is formed in the upper storage cover, and the required PRP amount is accurately extracted through the suction opening through the needle head. According to the blood separation device, two times of centrifugal separation can be carried out under the condition that blood is not exposed to air, the blood separation concentration is high, and the PRP extraction amount is accurate.

The invention relates to the technical field of biology, and discloses a method for detecting the activity of a fibrinolytic inhibitor activated by blood thrombin, the detection method comprises reagents of an experimental group, a control group and a blank group, a sample to be detected is platelet-deficient plasma, the three groups of samples are added into the reagents of the experimental group, the control group and the blank group respectively, then absorbance detection is performed, and the activity of the fibrinolytic inhibitor activated by the blood thrombin is detected. The activity of the thrombin-activated fibrinolytic inhibitor can be obtained by calculating absorbance data obtained through absorbance detection. The method for detecting the activity of the thrombin-activated fibrinolytic inhibitor comprises the following steps: dynamically monitoring a curve of the absorbance of plasma along with time in real time, comparing the curve difference of whether an inhibitor of the thrombin-activated fibrinolytic inhibitor is added or not, and calculating related parameters to represent the activity of the thrombin-activated fibrinolytic inhibitor. The operation is convenient; the sample is easy to obtain and the like.

The utility model discloses a device for preparing platelet-rich plasma suspension and gel containing blood cells. Comprising a fixing frame, at least one rapid clamping pipe clamp installed on the fixing frame, a vacuum centrifugal pipe clamped in the rapid clamping pipe clamp, and two suction devices capable of being inserted into the centrifugal pipe from the top or the bottom of the centrifugal pipe. The pusher is used for pushing the aspirator to be inserted into the centrifugal tube; the rotating mechanism is used for driving the pusher to rotate so as to enable the aspirator to face the top or the bottom of the centrifugal tube; the utility model solves the problems of blood pollution, manual operation error and low extraction rate in the manual operation process in the prior art, retains all blood platelets and white blood cells in the blood, retains red blood cells in normal proportion and most plasma proteins, and improves the extraction efficiency. Moreover, the concentration of platelets and leukocytes is constantly about 4 times of the concentration of blood of common normal people, the functions of erythrocyte components are reserved, and the functional effects of various components in the blood can be better exerted.

The invention discloses a PRP (platelet-rich plasma) stem cell exosome chitosan composition as well as a preparation method and application thereof. The PRP stem cell exosome chitosan composition comprises the following components: activated platelet-rich plasma, stem cell exosome and chitosan. The preparation method of the composite gel comprises the following steps: adding chitosan into a suspension containing activated platelet-rich plasma and stem cell exosome, uniformly mixing, adding a povidone suspending aid, oscillating to form a stable suspension state, adjusting the pH value to 7.5-7.8, mixing with thrombin and a calcium agent, stirring in a dark place, and solidifying to form a gelatinous substance, thereby obtaining the composite gel. The platelet-rich plasma-stem cell exosome-chitosan composite gel is obtained. The composition and the composite gel can promote microvascular endothelial cell regeneration and recover normal vascular endothelial morphology and functions; skin regeneration is promoted; broad-spectrum bacteriostasis and lipid reduction are realized, and local inflammation and immune response are inhibited.

To provide a specimen measurement device and a specimen measurement method that readily confirms the suitability of a sample as platelet-rich plasma.SOLUTION: A specimen measurement device 1 comprises a test section 13 for measuring optical information of platelet-rich plasma and platelet-poor plasma prepared from a blood specimen, a control sections 210 and 213 for acquiring information regarding platelet aggregation of the blood specimen based on the optical information of the platelet-rich plasma and platelet-poor plasma, and a display 4 for displaying information regarding platelet aggregation. The control sections 210 and 213 acquire evaluation information regarding the suitability of the platelet-rich plasma as a sample based on the measurement results of the platelet-rich plasma by the test section 13, and make the display 4 display the information.SELECTED DRAWING: Figure 15

The multi-layer structure platelet-rich plasma preparation tube comprises a vacuum blood collection tube body, a rubber plug is installed at the top end of the vacuum blood collection tube body, and a calcium gluconate solution layer is arranged at the bottom in the vacuum blood collection tube body. A separation gel layer II is arranged on the upper side of the calcium gluconate solution layer in the vacuum blood collection tube body, and a vitamin C solution layer is arranged on the upper side of the separation gel layer II in the vacuum blood collection tube body. The invention has the beneficial effects that through the design of the separation gel layer I and the separation gel layer II, the effective isolation of the vitamin C solution layer and the calcium gluconate solution layer from the ACDA solution layer before centrifugal separation is realized, chemical reaction or degradation is avoided, and a mixture of vitamin C and PRP is conveniently and quickly obtained through centrifugal operation after blood drawing, so that the blood drawing efficiency is improved. The method is used for tissue repair, regeneration and whitening, the operation process is simplified, and the product quality and safety are improved.

The present invention belongs to the field of preparation of diabetes complication remission agents, and particularly relates to a combined preparation for improving microcirculation of diabetic foot and a preparation method thereof. Starting from platelet-rich plasma, the present invention obtains exosomes containing rich active substances such as growth factors through stimulation with calcium gluconate and the like, and loads protamine nanoparticles to obtain bioactive substances with strong antibacterial ability, which can resist the development process of diabetic foot ulcers. The present invention uses bioactive glass, hydroxyethyl cellulose and the like to stabilize the bioactive substances, prolong the in vitro lifespan of the bioactive substances, and modified carboxymethyl cellulose provides better biodegradation effect. The combined preparation prepared by the present invention has less cytotoxicity, better antibacterial effect, can promote wound healing quickly, and the microcirculation index shows that it can stimulate the change of foot blood flow and improve the condition of foot artery blockage, etc.

A sample measuring apparatus according to an embodiment may be a sample measuring apparatus for measuring a platelet aggregation function of a blood sample. The apparatus may include: a detection section configured to measure optical information of platelet rich plasma and platelet poor plasma prepared from a blood sample; a control section configured to acquire information on a platelet aggregation function of the blood sample based on optical information of platelet rich plasma and platelet poor plasma; and a display configured to display information on a platelet aggregation function. The controller is configured to obtain evaluation information on suitability of the platelet-rich plasma as a sample based on a measurement result of the platelet-rich plasma by the detection portion, and display the evaluation information on the display.

A system and method of isolating extracellular vesicles. The method includes loading one or more of blood or bone marrow into an input port of a concentration system and centrifuging one or more of the blood or bone marrow to separate one or more of red blood cells, platelet poor plasma, or platelet rich plasma / bone marrow concentrate fractions via a centrifuge device. The method further includes pumping one or more of bone marrow / platelet rich plasma fractions and platelet poor plasma fractions into a receptacle of the concentration system and adding a concentrated aqueous two-phase solution to one or more of the bone marrow concentrate / platelet rich plasma fractions and platelet poor plasma fractions. The method also includes drawing the concentrated aqueous two-phase solution and one or more of the bone marrow concentrate / platelet rich plasma fractions or platelet poor plasma fractions back into the centrifuge device to isolate one or more of extracellular vesicles and platelet rich plasma / bone marrow concentrate fractions.

The application belongs to the technical field of cell culture, and particularly relates to umbilical cord blood-derived endothelial cell culture additives, an endothelial cell culture method and application. The umbilical cord blood-derived endothelial cell culture additives comprise activated platelet-rich plasma and at least one of miR-132 and miR-193b-3p high expression and miR-155 low expression exosomes. The application further provides an endothelial cell culture medium comprising the umbilical cord blood-derived endothelial cell culture additives, and the culture medium can significantly improve the growth state of HUVEC cells and effectively reduce or even avoid the vacuolization phenomenon in the culture process.

A platelet-rich plasma (PRP) activation system for activating PRP contained in a container includes a housing, a power supply, a piezoelectric transducer array, a support structure, and a coupling medium. The power supply is located in the housing. The piezoelectric transducer array is operably connected to the power supply and is located in the housing. The piezoelectric transducer array is configured to generate a focused shock wave using power from the power supply. The support structure is operably connected to the housing and is configured (i) to receive the container, and (ii) to position the container at least partially within the housing, such that at least a portion of the PRP is located at a focal volume formed by the focused shock wave. The coupling medium is located in the housing and is positioned between the piezoelectric transducer array and the container.

The invention relates to the technical field of biological medicine, and discloses a platelet-rich plasma exosome gel preparation method, which comprises: carrying out exosome separation on a PRP sample by using a high-speed centrifuge; stuffing the exosome precipitate into the shell-sphere structure to prepare core-shell structure particles so as to coat the exosome and form a thermal insulation layer; mixing thrombin and calcium gluconate according to a mass ratio of 10: 1, and adding the mixture into PRP to prepare a gel matrix; adding a photoinitiator, the magnetic coloring particles and the core-shell structure particles into the gel matrix, and uniformly mixing to prepare exosome gel; the invention further discloses application of the composition in repairing diabetic foot wounds. According to the invention, the irreversible influence of temporary environment change on the system can be weakened, meanwhile, the double mechanisms of healing are disclosed, during application, secondary arrangement is carried out by using a magnetic field, the release efficiency of PRP-Exo is improved under the later photo-thermal cooperation effect, and accurate formulation of a long-term treatment strategy is facilitated.

The utility model discloses an instrument for negative pressure extraction and filtration of thrombus of an artery. Comprising a sheathing canal, a blocking balloon, a blood collection bag, a pipeline and a negative pressure providing structure, the blocking balloon is arranged on the outer side of the thrombus treatment sheathing canal, then the blood collection bag is connected to the outer side of the sheathing canal through the pipeline, the sheathing canal is connected with the blood collection bag through the pipeline, and a thrombus filter screen is arranged in the pipeline; a negative pressure providing structure is further arranged, the negative pressure providing structure is a structure with a cavity which is arranged on the outer side of the blood collection bag and used for constructing negative pressure by extracting gas in the blood collection bag, power is provided through the negative pressure to lead blood in the arterial blood vessel out into the blood collection bag, and thrombus is blocked at the position of the filter screen after the blood passes through the thrombus filter screen. The filtered safe blood flows into the blood collecting bag, the blood in the blood collecting bag can be transfused back to the patient again in a transfusing mode, and the blood in the blood collecting bag can also provide a blood source for substances for extracting platelet rich plasma (PRP) therapy.

The invention provides a triple therapy medicine composition for treating chronic knee joint injury and a preparation method and an injection product thereof, and belongs to the technical field of medical biology. The invention provides a triple therapy pharmaceutical composition for treating knee joint injury. The pharmaceutical composition consists of the following components in parts by volume: 2-5 parts of platelet-rich plasma, 2-5 parts of mesenchymal stem cells and 2-5 parts of exosomes. The platelet-rich plasma, the mesenchymal stem cells and the exosome promote each other, so that the effects of relieving pain and promoting cartilage repair are superimposed and strengthened, the symptoms and functions are improved, the disease progression is delayed or partially reversed, and a relatively good effect on treating knee joint injury is achieved.

The invention relates to the technical field of hydrogel, in particular to bioactive bionic gradient hydrogel as well as a preparation method and application thereof.The preparation method comprises the following steps: dissolving quaternary ammonium salt chitosan, 3-acrylamidophenylboronic acid, platelet-rich plasma and phenyl (2, 4, 6-trimethylbenzoyl) lithium phosphate in deionized water, stirring until complete dissolution, centrifuging, and drying to obtain a hydrogel solution containing chitosan, 3-acrylamidophenylboronic acid, platelet-rich plasma and phenyl (2, 4, 6-trimethylbenzoyl) lithium phosphate; pre-polymerization liquid is obtained; preparing a tannic acid-iron ion (TA-Fe < 3 + >) mixed solution; adding the pre-polymerized liquid into a mold, and carrying out ultraviolet radiation crosslinking to obtain hydrogel; the hydrogel part is soaked in a tannic acid-iron ion mixed solution, and the gradient hydrogel is obtained. According to the biologically active bionic gradient hydrogel as well as the preparation method and the application thereof, the dual-network hydrogel is soaked in a TA-Fe < 3 + > solution to form a TA-Fe < 3 + > coordinated concentration gradient, so that the hard-to-soft mechanical gradient of tissues such as bone-cartilage, rotator cuff, achilles tendon and the like is accurately simulated.

The utility model provides a blood sampling and centrifuging integrated platelet-rich plasma preparation device which comprises a centrifugal sleeve and a blood sampling assembly coaxially and detachably installed in the centrifugal sleeve, and the blood sampling assembly comprises a blood collecting tube and a vein puncture needle connected to the head end of the blood collecting tube; the outer wall of the blood collecting tube is provided with a needle head containing bin used for containing a needle head of the vein puncture needle. According to the blood collection and centrifugation integrated platelet-rich plasma preparation device, a container does not need to be replaced in the blood collection and centrifugation process, and contact between the blood and the outside is reduced; and the needle head of the vein puncture needle can be properly stored, so that bacterial contamination is really avoided. Moreover, the needle head is accommodated in the needle head accommodating bin, so that the requirement on the length of the centrifugal sleeve can be reduced, and the blood carrying amount of the centrifugal sleeve with the same size is more.

The invention relates to the technical field of biology, and discloses a method for detecting thrombin production promotion capability of extracellular vesicles in a blood sample, which comprises the following steps: centrifuging the blood sample collected within 2 hours at 2500g at room temperature for 15 minutes, sucking upper platelet-deficient plasma and placing in a centrifuge tube, centrifuging at 2500g at room temperature again for 15 minutes, and sucking upper platelet-free plasma; centrifuging the obtained platelet-free plasma at 4 DEG C at 20000g for 1 hour; and sucking the upper-layer extracellular vesicle-free plasma for later use, taking the obtained extracellular vesicle precipitate as a to-be-detected sample, and detecting the thrombin generation promotion capability of the extracellular vesicles by using a thrombin generation analyzer. The method provided by the invention can be used for accurately detecting the capability of the extracellular vesicles in the blood sample for promoting thrombin generation, has the advantages of convenience in operation, easiness in obtaining the sample, noninvasiveness, no damage, high detection speed, low cost and the like, has important significance on thrombus risk assessment and prediction, and has better clinical application value.

The present invention discloses a method for separating components after centrifugation of blood cells, which belongs to the field of medical technology. It is achieved by utilizing the photosensitivity of the sensor and the difference in relative light absorption of each component after blood cell separation; after centrifugation, the blood cells are separated into a red blood cell layer, a buffy coat, and platelet-poor plasma. When each phase passes through the sensor, the relative light absorption characteristics of each phase are different, thereby accurately monitoring the blood layer. When each phase of the present invention passes through the sensor, the light absorption characteristics of the blood components are different, and accurate blood layer monitoring is performed based on this principle. The composition of the prepared product phase and subphase can be changed by changing the preset hematocrit. The sensor pipeline part that matches the sensor is a flat pipeline with a cross-section that is close to a large and flat rounded rectangle. It is made of a material with good light transmittance such as polycarbonate, so that light of different wavelengths emitted by the LED light emitter can pass through the flat pipeline vertically and be received by the light receiver.